US20050009148A1 - Glycosylated, low antigenicity, low immunogenicity factor VIII - Google Patents
Glycosylated, low antigenicity, low immunogenicity factor VIII Download PDFInfo
- Publication number
- US20050009148A1 US20050009148A1 US10/848,821 US84882104A US2005009148A1 US 20050009148 A1 US20050009148 A1 US 20050009148A1 US 84882104 A US84882104 A US 84882104A US 2005009148 A1 US2005009148 A1 US 2005009148A1
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- US
- United States
- Prior art keywords
- fviii
- factor viii
- low
- antigenicity
- hemophilia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S930/00—Peptide or protein sequence
- Y10S930/01—Peptide or protein sequence
- Y10S930/10—Factor VIII, AHF; related peptides
Definitions
- Hemophilia A is defined as hereditary deficiency of blood coagulation fVIII.
- FVIII is synthesized as a ⁇ 300 kDa single chain protein with internal sequence homology that defines the “domain” sequence NH 2 -A1-A2-B-A3-C1-C2-COOH (FIG. 1) (Vehar et al. [1984] Nature 312:337-342). Domains are commonly delineated as A1 (Ala1-Arg372), A2 (Ser373-Arg740), B (Ser741-Arg1648), and A3-C1-C2 (Ser1690-Tyr2332) (Eaton et al. [1986] Biochem. 25:8343-8347). Despite its large size, the B domain of fVIII has no known function and can be deleted. FVIII is measured by its ability to correct the prolonged clotting time of plasma prepared from patients with hemophilia A.
- Hemophilia A which is due to fVIII deficiency, is an X-linked, recessive disorder that is the most common severe, hereditary bleeding disorder in man.
- the mainstay of management of hemophilia A is fVIII replacement therapy by intravenous infusion.
- Current products in the marketplace include recombinant fVIII, immunoaffinity-purified plasma-derived fVIII, and intermediate-purity plasma-derived fVIII.
- inhibitory antibodies to fVIII is a serious complication in the management of patients with hemophilia A. Alloantibodies develop in approximately 25% of patients with hemophilia A in response to therapeutic infusions of fVIII (Aledort, L. [1994] Am. J. Hematol. 47:208-217). In previously untreated patients with hemophilia A who develop inhibitors, the inhibitor usually develops within one year of treatment (Lusher et al. [1993] N. Engl. J. Med. 328:453-459), although it can occur at any time (McMillan et al. [1988] Blood 71:344-348).
- autoantibodies that inactivate fVIII can occur in non-hemophiliacs in a variety of clinical settings including the postpartum period, in systemic lupus erythematosus, in chronic lymphocytic leukemia, and in elderly females. This condition is called acquired hemophilia.
- FVIII inhibitors are measured clinically by the ability of the patient's plasma to inhibit fVIII in normal plasma.
- the standard test is the Bethesda assay (Kasper et al. [1975] Thromb. Diath. Haemorr. 34:869-872).
- One Bethesda unit is defined as the dilution of patient plasma required to reduce the fVIII level by 50%.
- a molecule is said to be antigenic when it binds to antibodies and immunogenic when it can induce an immune response.
- the immunogenicity of a molecule depends on the B cell repertoire, T cell help and suppression, and the major histocompatibility complex, which together determine the concentration and binding affinity of antibodies for an antigenic site. If a fVIII molecule could be constructed that did not bind to the inhibitory antibodies in a patient's plasma, it would be useful therapeutically. Additionally, if a fVIII molecule could be constructed that is less immunogenic than wild-type human fVIII, i.e., could significantly lower the 25% incidence of inhibitor development, it would be safer than wild-type human fVIII. This molecule would have general applicability in the hemophilia A population.
- Inhibitory antibodies to fVIII bind to either the A2, A3, or C2 domains of fVIII and disrupt specific functions associated with these domains (Fulcher et al. [1985] Proc. Natl. Acad. Sci. USA 82:7728-7732; Scandella, et al. [1988] Proc. Natl. Acad. Sci. USA 85-6152-6156; Scandella et al. [1993] Blood 82:1767-1775).
- the A2 epitope is located within a linear sequence bounded by residues Arg484-Ile508 (Healey et al. [1995] J. Biol. Chem. 270:14505-14509).
- the C2 epitope has been localized to a sequence bounded by residues Glu2181-Val2243 (Healey et al. [1998] Blood 92:3701-3709).
- the A3 epitope has not yet been mapped.
- fVIII epitopes are limited in number and can be mapped to the amino acid sequence level makes it possible to design strategies to produce low antigenicity and low immunogenicity fVIII molecules.
- We have already reduced the antigenicity of fVIII by replacing epitopes with non-human fVIII sequences (Lubin et al. [1994] J. Biol. Chem. 269:8639-8641; Healy et al.
- HIV human immunodeficiency virus
- gp120 an exterior envelope glycoprotein
- HIV reduces the immunogenicity of gp120 using a post-translational process in which a polysaccharide is linked to asparagine residues. This process is called N-linked glycosylation because N is the single letter code for the amino acid asparagine.
- the immune system makes antibodies to the existing glycosylated epitope
- HIV responds by mutation vary its N-linked glycosylation sites. This reduces the immunogenicity of the virus.
- the immunogenicity of fVIII could be reduced by altering the epitope by glycosylation.
- the structure recognized by existing antibodies would be altered, reducing the antigenicity of the molecule.
- the fVIII cDNA is modified to code for amino acids within known, existing epitopes to produce a recognition sequence for glysosylation at asparagine residues.
- the consensus amino acid sequence for N-linked glycosylation is N-X-S/T, where N is asparagine, X is any amino acid, S/T stands for serine and threonine.
- Modification of the cDNA is accomplished by site-directed mutagenesis using standard methods. Thus, any three residue sequence in fVIII can be altered to N-X-S/T to produce the desired recognition site. Alternatively, a sequence containing a serine or threonine can be altered by mutating a single site to asparagine to produce the desired N-X-S/T sequence.
- the fVIII cDNA is inserted into a mammalian expression vector, which then is stably integrated into the genome of a mammalian host cell in culture. FVIII is secreted into the cell culture medium and purified. It is tested for antigenicity by measuring whether it is inhibited by inhibitory antibodies to fVIII that are obtained from patients. It is tested for immunogenicity by infusing it into hemophilia A mice and determining whether inhibitory antibodies develop.
- This mutation was introduced by site-directed mutagenesis of the human B-domainless fVIII cDNA.
- the cDNA sequence corresponding to residues 484-508 is shown below.
- the DNA sequence is SEQ ID NO: 1; the translated, unmodified amino acid sequence is SEQ ID NO:2. 484 AAC CGT CCT TTG TAT TCA AGG AGA TTA CCA AAA R P L Y S R R L P K 508 GGT GTA AAA CAT TTG AAG GAT TTT CCA AAT CTG CCA GGA GAA ATA G V K H L K D F P I L P G E I
- the fVIII mutant cDNA contained in the mammalian expression vector ReNeo (Lubin et al. [1994] supra), was transfected into COS-7 monkey cells for initial characterization. It was then stably transfected into baby hamster kidney cells using geneticin selection as described previously (Lubin et al. [1994] supra; Healey et al. [1995] supra). The transformed cells expressed active fVIII.
- an N-linked glycosylation site was introduced into the C2 epitope.
- DNA encoding glutamine 2189 was mutated to encode asparagine, generating an asparagine-isoleucine-threonine amino acid sequence which is a recognition site for glycosylation at amino acid residue 2189.
Abstract
The development of inhibitory antibodies to blood coagulation factor VIII (fVIII) results in a severe bleeding tendency. These antibodies arise in patients with hemophilia A (hereditary fVIII deficiency) who have been transfused with fVIII. They also occur in non-hemophiliacs, which produces the condition acquired hemophilia. We describe a method to construct and express novel recombinant fVIII molecules which escape detection by existing inhibitory antibodies (low antigenicity fVIII) and which decrease the likelihood of developing inhibitory antibodies (low immunogenicity fVIII). In this method, fVIII is glycosylated at sites that are known to be antibody recognition sequences (epitopes). This produces the desired properties of low antigenicity fVIII and low immunogenicity fVIII. The mechanism is similar to one used by viruses such as the AIDS virus, which glycosylates its surface proteins to escape detection by the immune system.
Description
- The present application is a continuation application of U.S. patent application Ser. No. 09/435,403 filed Nov. 5, 1999, which claims benefit of U.S. Provisional Patent Application Ser. No. 60/107,402 filed Nov. 6, 1998, which is incorporated herein in its entirety, by reference.
- Hemophilia A is defined as hereditary deficiency of blood coagulation fVIII. FVIII is synthesized as a ˜300 kDa single chain protein with internal sequence homology that defines the “domain” sequence NH2-A1-A2-B-A3-C1-C2-COOH (FIG. 1) (Vehar et al. [1984] Nature 312:337-342). Domains are commonly delineated as A1 (Ala1-Arg372), A2 (Ser373-Arg740), B (Ser741-Arg1648), and A3-C1-C2 (Ser1690-Tyr2332) (Eaton et al. [1986] Biochem. 25:8343-8347). Despite its large size, the B domain of fVIII has no known function and can be deleted. FVIII is measured by its ability to correct the prolonged clotting time of plasma prepared from patients with hemophilia A.
- Hemophilia A, which is due to fVIII deficiency, is an X-linked, recessive disorder that is the most common severe, hereditary bleeding disorder in man. The mainstay of management of hemophilia A is fVIII replacement therapy by intravenous infusion. Current products in the marketplace include recombinant fVIII, immunoaffinity-purified plasma-derived fVIII, and intermediate-purity plasma-derived fVIII.
- The development of inhibitory antibodies (inhibitors) to fVIII is a serious complication in the management of patients with hemophilia A. Alloantibodies develop in approximately 25% of patients with hemophilia A in response to therapeutic infusions of fVIII (Aledort, L. [1994] Am. J. Hematol. 47:208-217). In previously untreated patients with hemophilia A who develop inhibitors, the inhibitor usually develops within one year of treatment (Lusher et al. [1993] N. Engl. J. Med. 328:453-459), although it can occur at any time (McMillan et al. [1988] Blood 71:344-348). Additionally, autoantibodies that inactivate fVIII can occur in non-hemophiliacs in a variety of clinical settings including the postpartum period, in systemic lupus erythematosus, in chronic lymphocytic leukemia, and in elderly females. This condition is called acquired hemophilia.
- FVIII inhibitors are measured clinically by the ability of the patient's plasma to inhibit fVIII in normal plasma. The standard test is the Bethesda assay (Kasper et al. [1975] Thromb. Diath. Haemorr. 34:869-872). One Bethesda unit is defined as the dilution of patient plasma required to reduce the fVIII level by 50%.
- A molecule is said to be antigenic when it binds to antibodies and immunogenic when it can induce an immune response. The immunogenicity of a molecule depends on the B cell repertoire, T cell help and suppression, and the major histocompatibility complex, which together determine the concentration and binding affinity of antibodies for an antigenic site. If a fVIII molecule could be constructed that did not bind to the inhibitory antibodies in a patient's plasma, it would be useful therapeutically. Additionally, if a fVIII molecule could be constructed that is less immunogenic than wild-type human fVIII, i.e., could significantly lower the 25% incidence of inhibitor development, it would be safer than wild-type human fVIII. This molecule would have general applicability in the hemophilia A population.
- Inhibitory antibodies to fVIII bind to either the A2, A3, or C2 domains of fVIII and disrupt specific functions associated with these domains (Fulcher et al. [1985] Proc. Natl. Acad. Sci. USA 82:7728-7732; Scandella, et al. [1988] Proc. Natl. Acad. Sci. USA 85-6152-6156; Scandella et al. [1993] Blood 82:1767-1775). The A2 epitope is located within a linear sequence bounded by residues Arg484-Ile508 (Healey et al. [1995] J. Biol. Chem. 270:14505-14509). The C2 epitope has been localized to a sequence bounded by residues Glu2181-Val2243 (Healey et al. [1998] Blood 92:3701-3709). The A3 epitope has not yet been mapped. The fact that fVIII epitopes are limited in number and can be mapped to the amino acid sequence level makes it possible to design strategies to produce low antigenicity and low immunogenicity fVIII molecules. We have already reduced the antigenicity of fVIII by replacing epitopes with non-human fVIII sequences (Lubin et al. [1994] J. Biol. Chem. 269:8639-8641; Healy et al. [1995] supra; Healey et al. [1998] supra) and by site-directed mutagenesis of amino acids within fVIII epitopes (Lubin et al. [1997] J. Biol. Chem. 272:30191-30195).
- Viruses, such as the human immunodeficiency virus (HIV), elude the immune system by varying epitopes that are recognized by antibodies (Wyatt et al. [1998] Nature 393:705-711). HIV contains an exterior envelope glycoprotein, gp120, which is targetted by the immune system in its attempts to rid the body of virus. HIV reduces the immunogenicity of gp120 using a post-translational process in which a polysaccharide is linked to asparagine residues. This process is called N-linked glycosylation because N is the single letter code for the amino acid asparagine. When the immune system makes antibodies to the existing glycosylated epitope, HIV responds by mutation vary its N-linked glycosylation sites. This reduces the immunogenicity of the virus. Similarly, the immunogenicity of fVIII could be reduced by altering the epitope by glycosylation. Additionally, the structure recognized by existing antibodies would be altered, reducing the antigenicity of the molecule.
- The fVIII cDNA is modified to code for amino acids within known, existing epitopes to produce a recognition sequence for glysosylation at asparagine residues. The consensus amino acid sequence for N-linked glycosylation is N-X-S/T, where N is asparagine, X is any amino acid, S/T stands for serine and threonine. Modification of the cDNA is accomplished by site-directed mutagenesis using standard methods. Thus, any three residue sequence in fVIII can be altered to N-X-S/T to produce the desired recognition site. Alternatively, a sequence containing a serine or threonine can be altered by mutating a single site to asparagine to produce the desired N-X-S/T sequence.
- The fVIII cDNA is inserted into a mammalian expression vector, which then is stably integrated into the genome of a mammalian host cell in culture. FVIII is secreted into the cell culture medium and purified. It is tested for antigenicity by measuring whether it is inhibited by inhibitory antibodies to fVIII that are obtained from patients. It is tested for immunogenicity by infusing it into hemophilia A mice and determining whether inhibitory antibodies develop.
- As an example of the method used to create glycosylated, low antigenicity, low immunogenicity fVIII, we describe the introduction of a recognition site for N-linked glycosylation at leucine 486 within the A2 epitope. FVIII contains a serine at position 488 within the A2 epitope. The 486-488 sequence is leu-tyr-ser. Therefore, mutation of leucine to asparagine produces a sequence N-Y-S (using the single letter code), which is a recognition site for N-linked glycosylation.
- This mutation was introduced by site-directed mutagenesis of the human B-domainless fVIII cDNA. The cDNA sequence corresponding to residues 484-508 is shown below. The DNA sequence is SEQ ID NO: 1; the translated, unmodified amino acid sequence is SEQ ID NO:2.
484 AAC CGT CCT TTG TAT TCA AGG AGA TTA CCA AAA R P L Y S R R L P K 508 GGT GTA AAA CAT TTG AAG GAT TTT CCA AAT CTG CCA GGA GAA ATA G V K H L K D F P I L P G E I
The nucleotide sequence TTG, coding for leucine, was changed to AAC, which codes for asparagine. - The fVIII mutant cDNA, contained in the mammalian expression vector ReNeo (Lubin et al. [1994] supra), was transfected into COS-7 monkey cells for initial characterization. It was then stably transfected into baby hamster kidney cells using geneticin selection as described previously (Lubin et al. [1994] supra; Healey et al. [1995] supra). The transformed cells expressed active fVIII.
- As a further example, an N-linked glycosylation site was introduced into the C2 epitope. DNA encoding glutamine 2189 was mutated to encode asparagine, generating an asparagine-isoleucine-threonine amino acid sequence which is a recognition site for glycosylation at amino acid residue 2189.
- It will be understood by those skilled in the art that other such modifications can be made within any of the domains giving rise to inhibitory analogs to provide N-linked glycosylation sites. Further, a plurality of such sites can be combined in a single fVIII molecule, so as to render the molecule unreactive (or less active than wild-type) to inhibitory antibodies. FVIII molecules modified according to this invention are also expected to have reduced immunogenicity.
Claims (4)
1. A method for preparing a biologically active factor VIII having modified glycosylation comprising the steps of
mutating a desired segment of factor VIII DNA to encode -N-X-S/T, where N is asparagine, X is any amino acid, and S/T is serine or threonine, thereby providing mutated factor VIII DNA encoding a post-translational glycosylation site at the desired locus of factor VIII protein, and
expressing the mutated DNA in a host cell capable of post-translational glycosylation, whereby biologically active factor VIII having modified glycosylation is prepared.
2. The method of claim 1 wherein said desired segment resides in the A2 domain.
3. The method of claim 1 wherein said desired segment resides in the C2 domain.
4. The method of claim 3 wherein said desired segment comprises the amino acid residue, glutamine, at position 2189 in the C2 domain.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/848,821 US20050009148A1 (en) | 1998-11-06 | 2004-05-19 | Glycosylated, low antigenicity, low immunogenicity factor VIII |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10740298P | 1998-11-06 | 1998-11-06 | |
US09/435,403 US6759216B1 (en) | 1998-11-06 | 1999-11-05 | Glycosylated, low antigenicity low immunogenicity factor VIII |
US10/848,821 US20050009148A1 (en) | 1998-11-06 | 2004-05-19 | Glycosylated, low antigenicity, low immunogenicity factor VIII |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
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US09/435,403 Continuation US6759216B1 (en) | 1998-11-06 | 1999-11-05 | Glycosylated, low antigenicity low immunogenicity factor VIII |
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US20050009148A1 true US20050009148A1 (en) | 2005-01-13 |
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Application Number | Title | Priority Date | Filing Date |
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US09/435,403 Expired - Fee Related US6759216B1 (en) | 1998-11-06 | 1999-11-05 | Glycosylated, low antigenicity low immunogenicity factor VIII |
US10/848,821 Abandoned US20050009148A1 (en) | 1998-11-06 | 2004-05-19 | Glycosylated, low antigenicity, low immunogenicity factor VIII |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
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US09/435,403 Expired - Fee Related US6759216B1 (en) | 1998-11-06 | 1999-11-05 | Glycosylated, low antigenicity low immunogenicity factor VIII |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20070173446A1 (en) * | 2004-05-03 | 2007-07-26 | Lollar John S | Method of administering porcine B-domainless fVIII |
US20090325881A1 (en) * | 1996-06-26 | 2009-12-31 | Emory University | Modified factor viii |
US9150637B2 (en) | 2010-11-05 | 2015-10-06 | Baxalta Inc. | Variant of antihemophilic factor VIII having increased specific activity |
JP2021052769A (en) * | 2015-02-06 | 2021-04-08 | ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル | Optimized human clotting factor viii gene expression cassettes and their use |
Families Citing this family (14)
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WO2003087161A1 (en) * | 2002-04-18 | 2003-10-23 | Merck Patent Gmbh | Modified factor viii |
AU2004296768B2 (en) * | 2003-12-03 | 2010-06-24 | University Of Rochester | Recombinant factor VIII having increased specific activity |
KR20180110192A (en) | 2004-11-12 | 2018-10-08 | 바이엘 헬스케어 엘엘씨 | Site-directed modification of fviii |
US20100256062A1 (en) | 2004-12-06 | 2010-10-07 | Howard Tommy E | Allelic Variants of Human Factor VIII |
WO2006103298A2 (en) * | 2005-04-01 | 2006-10-05 | Novo Nordisk Health Care Ag | Blood coagulation fviii analogues |
FR2913020B1 (en) * | 2007-02-23 | 2012-11-23 | Biomethodes | NEW VIII FACTORS FOR THE TREATMENT OF TYPE A HEMOPHILS |
EP1985631A1 (en) * | 2007-04-20 | 2008-10-29 | LFB Biotechnologies | Demannosylated recombinant factor VIII for the treatment of patients with hemophiila A |
AU2008319183B2 (en) | 2007-11-01 | 2014-09-04 | University Of Rochester | Recombinant factor VIII having increased stability |
CN102137935A (en) * | 2008-06-25 | 2011-07-27 | 拜耳医药保健有限公司 | Factor VIII muteins with reduced immunogenicity |
WO2011088391A2 (en) * | 2010-01-14 | 2011-07-21 | Haplomics, Inc. | Predicting and reducing alloimmunogenicity of protein therapeutics |
US20130040888A1 (en) * | 2010-02-16 | 2013-02-14 | Novo Nordisk A/S | Factor VIII Molecules With Reduced VWF Binding |
AU2011303916A1 (en) | 2010-09-15 | 2013-03-21 | Novo Nordisk A/S | Factor VIII variants having a decreased cellular uptake |
BR112015013311A2 (en) | 2012-12-07 | 2017-11-14 | Haplomics Inc | tolerance induction and factor 8 mutation repair |
ES2837475T3 (en) | 2013-06-24 | 2021-06-30 | Xiao Weidong | Mutant Factor VIII Compositions and Methods |
Citations (3)
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US5041376A (en) * | 1988-12-09 | 1991-08-20 | The Board Of Regents Of The University Of Texas System | Method for identifying or shielding functional sites or epitopes of proteins that enter the exocytotic pathway of eukaryotic cells, the mutant proteins so produced and genes encoding said mutant proteins |
US5585250A (en) * | 1993-08-20 | 1996-12-17 | The United States Of America As Represented By The Department Of Health & Human Services | Dampening of an immunodominant epitope of an antigen for use in plant, animal and human compositions and immunotherapies |
US5859204A (en) * | 1992-04-07 | 1999-01-12 | Emory University | Modified factor VIII |
-
1999
- 1999-11-05 US US09/435,403 patent/US6759216B1/en not_active Expired - Fee Related
-
2004
- 2004-05-19 US US10/848,821 patent/US20050009148A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5041376A (en) * | 1988-12-09 | 1991-08-20 | The Board Of Regents Of The University Of Texas System | Method for identifying or shielding functional sites or epitopes of proteins that enter the exocytotic pathway of eukaryotic cells, the mutant proteins so produced and genes encoding said mutant proteins |
US5859204A (en) * | 1992-04-07 | 1999-01-12 | Emory University | Modified factor VIII |
US5585250A (en) * | 1993-08-20 | 1996-12-17 | The United States Of America As Represented By The Department Of Health & Human Services | Dampening of an immunodominant epitope of an antigen for use in plant, animal and human compositions and immunotherapies |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090325881A1 (en) * | 1996-06-26 | 2009-12-31 | Emory University | Modified factor viii |
US8951515B2 (en) | 1996-06-26 | 2015-02-10 | Emory University | Modified factor VIII |
US20070173446A1 (en) * | 2004-05-03 | 2007-07-26 | Lollar John S | Method of administering porcine B-domainless fVIII |
US7576181B2 (en) | 2004-05-03 | 2009-08-18 | Ipsen Biopharm Limited | Method of administering porcine B-domainless fVIII |
US20090270329A1 (en) * | 2004-05-03 | 2009-10-29 | Emory University | Methods of administering porcine b-domainless fviii |
US8101718B2 (en) | 2004-05-03 | 2012-01-24 | Emory University | Methods of administering porcine B-domainless fVIII |
US8501694B2 (en) | 2004-05-03 | 2013-08-06 | Emory University | Method of administering porcine B-domainless fVIII |
US9150637B2 (en) | 2010-11-05 | 2015-10-06 | Baxalta Inc. | Variant of antihemophilic factor VIII having increased specific activity |
US10053500B2 (en) | 2010-11-05 | 2018-08-21 | Baxalta Incorporated | Variant of antihemophilic factor VIII having increased specific activity |
JP2021052769A (en) * | 2015-02-06 | 2021-04-08 | ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル | Optimized human clotting factor viii gene expression cassettes and their use |
JP7437035B2 (en) | 2015-02-06 | 2024-02-22 | ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル | Optimized human coagulation factor VIII gene expression cassette and its use |
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