US20040266989A1

US20040266989A1 - Methods and compositions for the treatment of gastrointestinal disorders

Info

Publication number: US20040266989A1
Application number: US10/766,735
Authority: US
Inventors: Mark Currie; Shalina Mahajan-Miklos
Original assignee: Microbia Inc
Current assignee: Microbia Inc
Priority date: 2003-01-28
Filing date: 2004-01-28
Publication date: 2004-12-30
Also published as: CY1113217T1; AU2009235993A1; BE2013C032I2; ES2285417T3; JP5748791B2; MXPA05008097A; SI1594517T1; HUS1300022I1; DK2246360T3; AU2009235993B2; EP1911763B1; JP2013116910A; BR122018074353B8; ATE477268T1; NZ570134A; DE602004007105D1; CY1111058T1; WO2004069165A3; DE602004007105T2; KR20050106404A

Abstract

The present invention features compositions and related methods for treating IBS and other gastrointestinal disorders and conditions (e.g., gastrointestinal motility disorders, functional gastrointestinal disorders, gastroesophageal reflux disease (GERD), Crohn's disease, ulcerative colitis, Inflammatory bowel disease, functional heartburn, dyspepsia (including functional dyspepsia or nonulcer dyspepsia), gastroparesis, chronic intestinal pseudo-obstruction (or colonic pseudo-obstruction), and disorders and conditions associated with constipation, e.g., constipation associated with use of opiate pain killers, post-surgical constipation, and constipation associated with neuropathic disorders as well as other conditions and disorders using peptides and other agents that activate the guanylate cyclase C (GC—C) receptor.

Description

CLAIM OF PRIORITY

This application claims priority under 35 USC § 119(e) to U.S. Provisional Patent Application Ser. No. 60/443,098, filed on Jan. 28, 2003; U.S. Provisional Patent Application Ser. No. 60/471,288, filed on May 15, 2003 and U.S. Provisional Patent Application Ser. No. 60/519,460, filed on Nov. 12, 2003, the entire contents of which are hereby incorporated by reference.[0001]

TECHNICAL FIELD

This invention relates to methods and compositions for treating various disorders, including gastrointestinal disorders, obesity, congestive heart failure and benign prostatic hyperplasia.

BACKGROUND

Irritable bowel syndrome (IBS) is a common chronic disorder of the intestine that affects 20 to 60 million individuals in the US alone (Lehman Brothers, Global Healthcare-Irritable bowel syndrome industry update, September 1999). IBS is the most common disorder diagnosed by gastroenterologists (28% of patients examined) and accounts for 12% of visits to primary care physicians (Camilleri 2001, Gastroenterology 120:652-668). In the US, the economic impact of IBS is estimated at $25 billion annually, through direct costs of health care use and indirect costs of absenteeism from work (Talley 1995, Gastroenterology 109:1736-1741). Patients with IBS have three times more absenteeism from work and report a reduced quality of life. Sufferers may be unable or unwilling to attend social events, maintain employment, or travel even short distances (Drossman 1993, Dig Dis Sci 38:1569-1580). There is a tremendous unmet medical need in this population since few prescription options exist to treat IBS.

Patients with IBS suffer from abdominal pain and a disturbed bowel pattern. Three subgroups of IBS patients have been defined based on the predominant bowel habit: constipation-predominant (c-IBS), diarrhea-predominant (d-IBS) or alternating between the two (a-IBS). Estimates of individuals who suffer from c-IBS range from 20-50% of the IBS patients with 30% frequently cited. In contrast to the other two subgroups that have a similar gender ratio, c-IBS is more common in women (ratio of 3:1) (Talley et al. 1995, Am J Epidemiol 142:76-83).

The definition and diagnostic criteria for IBS have been formalized in the “Rome Criteria” (Drossman et al. 1999, Gut 45:Suppl II: 1-81), which are well accepted in clinical practice. However, the complexity of symptoms has not been explained by anatomical abnormalities or metabolic changes. This has led to the classification of IBS as a functional GI disorder, which is diagnosed on the basis of the Rome criteria and limited evaluation to exclude organic disease.(Ringel et al. 2001, Annu Rev Med 52: 319-338). IBS is considered to be a “biopsychosocial” disorder resulting from a combination of three interacting mechanisms: altered bowel motility, an increased sensitivity of the intestine or colon to pain stimuli (visceral sensitivity) and psychosocial factors (Camilleri 2001, Gastroenterology 120:652-668). Recently, there has been increasing evidence for a role of inflammation in etiology of IBS. Reports indicate that subsets of IBS patients have small but significant increases in colonic inflammatory and mast cells, increased inducible nitric oxide (NO) and synthase (iNOS) and altered expression of inflammatory cytokines (reviewed by Talley 2000, Medscape Coverage of DDW week).

SUMMARY

The present invention features compositions and related methods for treating IBS and other gastrointestinal disorders and conditions (e.g., gastrointestinal motility disorders, functional gastrointestinal disorders, gastroesophageal reflux disease (GERD), Crohn's disease, ulcerative colitis, Inflammatory bowel disease, functional heartburn, dyspepsia (including functional dyspepsia or nonulcer dyspepsia), gastroparesis, chronic intestinal pseudo-obstruction (or colonic pseudo-obstruction), and disorders and conditions associated with constipation, e.g., constipation associated with use of opiate pain killers, post-surgical constipation, and constipation associated with neuropathic disorders as well as other conditions and disorders. The compositions feature peptides that activate the guanylate cyclase C (GC—C) receptor.

The present invention also features compositions and related methods for treating obesity, congestive heart failure and benign prostatic hyperplasia (BPH).

Without being bound by any particular theory, in the case of IBS and other gastrointestinal disorders the peptides are useful because they can increase gastrointestinal motility.

Without being bound by any particular theory, in the case of IBS and other gastrointestinal disorders the peptides are useful, in part, because they can decrease inflammation.

Without being bound by any particular theory, in the case of IBS and other gastrointestinal disorders the peptides are also useful because they can decrease gastrointestinal pain or visceral pain.

The invention features pharmaceutical compositions comprising certain peptides that are capable of activating the guanylate-cyclase C (GC—C) receptor. Also within the invention are pharmaceutical compositions comprising a peptide of the invention as well as combination compositions comprising a peptide of the invention and a second therapeutic agent, e.g., an agent for treating constipation (e.g., SPI-0211; Sucampo Pharmaceuticals, Inc.; Bethesda, Md.) or some other gastrointestinal disorder. Examples of a second therapeutic agent include: acid reducing agents such as proton pump inhibitors and H2 receptor blockers, pro-motility agents such as 5HT receptor agonists (e.g. Zelnorm®), anti-inflammatory agents, antispasmodics, antidepressants, centrally-acting analgesic agents such as opiod receptor agonists, opiod receptor antagonists, agents for the treatment of Inflammatory bowel disease, Crohn's disease and ulcerative colitis (e.g., Traficet-EN™ (ChemoCentryx, Inc.; San Carlos, Calif.) agents that treat gastrointestinal or visceral pain and cGMP phosphodiesterase inhibitors (motapizone, zaprinast, and suldinac sulfone). Thus, for example, the pharmaceutical compositions can include an analgesic agent selected from the group consisting of: Ca channel blockers (e.g., ziconotide), 5HT receptor antagonists (for example 5HT3, 5HT4 and 5HT1 receptor antagonists), opioid receptor agonists (e.g., loperamide, fedotozine, and fentanyl, naloxone, naltrexone, methyl nalozone, nalmefene, cypridime, beta funaltrexamine, naloxonazine, naltrindole, and nor-binaltorphimine, morphine, diphenyloxylate, enkephalin pentapeptide, and trimebutine), NK1 receptor antagonists (e.g., ezlopitant and SR-14033), CCK receptor agonists (e.g., loxiglumide), NK1 receptor antagonists, NK3 receptor antagonists (e.g., talnetant, osanetant (SR-142801)), norepinephrine-serotonin reuptake inhibitors (NSRI; e.g., milnacipran), vanilloid and cannabanoid receptor agonists (e.g., arvanil), sialorphin, sialorphin-related peptides comprising the amino acid sequence QHNPR (SEQ ID NO:111) for example, VQHNPR (SEQ ID NO:112); VRQHNPR (SEQ ID NO:113); VRGQHNPR (SEQ ID NO:114); VRGPQHNPR (SEQ ID NO:115); VRGPRQHNPR (SEQ ID NO:116); VRGPRRQHNPR (SEQ ID NO:117); and RQHNPR (SEQ ID NO:118), compounds or peptides that are inhibitors of neprilysin, frakefamide (H-Tyr-D-Ala-Phe(F)-Phe-NH ₂; WO 01/019849 A1), loperamide, Tyr-Arg (kyotorphin), CCK receptor agonists (caerulein), conotoxin peptides, peptide analogs of thymulin, loxiglumide, dexloxiglumide (the R-isomer of loxiglumide) (WO 88/05774) and other analgesic peptides or compounds can be used with or linked to the peptides of the invention.

The invention includes methods for treating various gastrointestinal disorders by administering a peptide that acts as a partial or complete agonist of the GC—C receptor. The peptide includes at least six cysteines that form three disulfide bonds. In certain embodiments the disulfide bonds are replaced by other covalent cross-links and in some cases the cysteines are substituted by other residues to provide for alternative covalent cross-links. The peptides may also include at least one trypsin or chymotrypsin cleavage site and/or a carboxy-terminal analgesic peptide or small molecule, e.g., AspPhe or some other analgesic peptide. When present within the peptide, the analgesic peptide or small molecule may be preceded by a chymotrypsin or trypsin cleavage site that allows release of the analgesic peptide or small molecule. The peptides and methods of the invention are also useful for treating pain and inflammation associated with various disorders, including gastrointestinal disorders. Certain peptides include a functional chymotrypsin or trypsin cleavage site located so as to allow inactivation of the peptide upon cleavage. Certain peptides having a functional cleavage site undergo cleavage and gradual inactivation in the digestive tract, and this is desirable in some circumstances. In certain peptides, a functional chymotrypsin site is altered, increasing the stability of the peptide in vivo.

The invention includes methods for treating other disorders such as congestive heart failure and benign prostatic hyperplasia by administering a peptide or small molecule (parenterally or orally) that acts as an agonist of the GC—C receptor. Such agents can be used in combination with natriuretic peptides (e.g., atrial natriuretic peptide, brain natriuretic peptide or C-type natriuretic peptide), a diuretic, or an inhibitor of angiotensin converting enzyme.

The invention features methods and compositions for increasing intestinal motility. Intestinal motility involves spontaneous coordinated dissentions and contractions of the stomach, intestines, colon and rectum to move food through the gastrointestinal tract during the digestive process.

In certain embodiments the peptides include either one or two or more contiguous negatively charged amino acids (e.g., Asp or Glu) or one or two or more contiguous positively charged residues (e.g., Lys or Arg) or one or two or more contiguous positively or negatively charged amino acids at the carboxy terminus. In these embodiments all of the flanking amino acids at the carboxy terminus are either positively or negatively charged. In other embodiments the carboxy terminal charged amino acids are preceded by a Leu. For example, the following amino acid sequences can be added to the carboxy terminus of the peptide: Asp; Asp Lys; Lys Lys Lys Lys Lys Lys (SEQ ID NO:123); Asp Lys Lys Lys Lys Lys Lys (SEQ ID NO:124); Leu Lys Lys; and Leu Asp. It is also possible to simply add Leu at the carboxy terminus.

In a first aspect, the invention features a peptide comprising, consisting of, or consisting essentially of the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:119) wherein: Xaa₁Xaa₂Xaa₃Xaa4 Xaa₅is Asn Ser Ser Asn Tyr (SEQ ID NO:121) or is missing or Xaa₁Xaa₂Xaa₃Xaa₄is missing. In certain embodiments Xaa₈, Xaa₉, Xaa₁₂, Xaa₁₃, Xaa₁₄, Xaa₁₇, and Xaa₁₉can be any amino acid. In certain embodiments Xaa₅is Asn, Trp, Tyr, Asp, or Phe. In other embodiments, Xaa₅can also be Thr or Ile. In other embodiments Xaa₅is Tyr, Asp or Trp. In some embodiments Xaa₈is Glu, Asp, Gln, Gly or Pro. In other embodiments Xaa₈is Glu; in some embodiments Xaa₉is Leu, Ile, Val, Ala, Lys, Arg, Trp, Tyr or Phe in some embodiments Xaa₉is Leu, Ile, Val, Lys, Arg, Trp, Tyr or Phe.

In certain embodiments, an amino acid can be replace by a non-naturally occurring amino acid or a naturally or non-naturally occurring amino acid analog. For example, an aromatic amino acid can be replaced by 3,4-dihydroxy-L-phenylalanine, 3-iodo-L-tyrosine, triiodothyronine, L-thyroxine, phenylglycine (Phg) or nor-tyrosine (norTyr). Phg and norTyr and other amino acids including Phe and Tyr can be substituted by, e.g., a halogen, —CH3, —OH, —CH ₂NH₃, —C(O)H, —CH₂CH₃, —CN, —CH₂CH₂CH₃, —SH, or another group.

In some embodiments Xaa ₁₂is Asn, Tyr, Asp or Ala. In other embodiments Xaa₁₂is Asn. In some embodiments Xaa₁₃is Ala, Pro or Gly, and in other embodiments it is Pro. In some embodiments Xaa₁₄is Ala, Leu, Ser, Gly, Val, Glu, Gln, Ile, Leu, Lys, Arg, or Asp, and in other embodiments it is Ala or Gly, and in still other embodiments it is Ala. In some embodiments Xaa₁₆is Thr, Ala, Asn, Lys, Arg, Trp; Xaa₁₇is Gly, Pro or Ala; Xaa₁₉is selected from Trp, Tyr, Phe, Asn and Leu or Xaa₁₉is selected from Tip, Tyr, and Phe or Xaa₁₉is selected from Leu, Ile and Val; or Xaa₁₉is His or Xaa₁₉is selected from Trp, Tyr, Phe, Asn, Ile, Val, His and Leu; and Xaa₂₀Xaa₂₁is AspPhe or is missing or Xaa₂₀is Asn or Glu and Xaa₂₁is missing or Xaa₁₉Xaa₂₀Xaa₂₁is missing. The invention also features methods for treating a gastrointestinal disorder (e.g., a gastrointestinal motility disorder, a functional gastrointestinal disorder, gastroesophageal reflux disease, functional heartburn, dyspepsia, functional dyspepsia, nonulcer dyspepsia, gastroparesis, chronic intestinal pseudo-obstruction, colonic pseudo-obstruction), obesity, congestive heart failure or benign prostatic hyperplasia by administering a composition comprising an aforementioned peptide

When Xaa ₉is Trp, Tyr or Phe or when Xaa₁₆is Trp the peptide has a potentially functional chymotrypsin cleavage site that is located at a position where cleavage will inactivate GC—C receptor binding by the peptide. When Xaa₉is Lys or Arg or when Xaa₁₆is Lys or Arg, the peptide has a potentially functional trypsin cleavage site that is located at a position where cleavage will inactivate GC—C receptor binding by the peptide.

When Xaa ₁₉is Trp, Tyr or Phe, the peptide has a chymotrypsin cleavage site that is located at a position where cleavage will liberate the portion of the peptide carboxy-terminal to Xaa₁₉. When Xaa₁₉is Leu, Ile or Val, the peptide can have a chymotrypsin cleavage site that is located at a position where cleavage will liberate the portion of the peptide amino-terminal to Xaa₁₉. At relatively high pH the same effect is seen when Xaa₁₉is His. When Xaa₁₉is Lys or Arg, the peptide has a trypsin cleavage site that is located at a position where cleavage will liberate portion of the peptide carboxy-terminal to Xaa₁₉. Thus, if the peptide includes an analgesic peptide carboxy-terminal to Xaa₁₉, the peptide will be liberated in the digestive tract upon exposure to the appropriate protease. Among the analgesic peptides which can be included in the peptide are: AspPhe (as Xaa₂₀Xaa₂₁), endomorphin-1, endomorphin-2, nocistatin, dalargin, lupron, and substance P and other analgesic peptides described herein. These peptides can, for example, be used to replace Xaa₂₀Xaa₂₁.

When Xaa ₁or the amino-terminal amino acid of the peptide of the invention (e.g., Xaa₂or Xaa₃) is Trp, Tyr or Phe, the peptide has a chymotrypsin cleavage site that is located at a position where cleavage will liberate the portion of the peptide amino-terminal to Xaa₁(or Xaa₂or Xaa₃) along with Xaa₁, Xaa₂or Xaa₃. When Xaa₁or the amino-terminal amino acid of the peptide of the invention (e.g., Xaa₂or Xaa₃) is Lys or Arg, the peptide has a trypsin cleavage site that is located at a position where cleavage will liberate portion of the peptide amino-terminal to Xaa₁along with Xaa₁, Xaa₂or Xaa₃). When Xaa₁or the amino-terminal amino acid of the peptide of the invention is Leu, Ile or Val, the peptide can have a chymotrypsin cleavage site that is located at a position where cleavage will liberate the portion of the peptide amino-terminal to Xaa₁. At relatively high pH the same effect is seen when Xaa₁is His. Thus, for example, if the peptide includes an analgesic peptide amino-terminal to Xaa₁, the peptide will be liberated in the digestive tract upon exposure to the, appropriate protease. Among the analgesic peptides which can be included in the peptide are: AspPhe, endomorphin-1, endomorphin-2, nocistatin, dalargin, lupron, and substance p and other analgesic peptides described herein.

When fully folded, disulfide bonds are present between: Cys ₆and Cys₁₁; Cys₇and Cys₁₅; and Cys₁₀and Cys₁₈. The peptides of the invention bear some sequence similarity to ST peptides. However, they include amino acid changes and/or additions that improve functionality. These changes can, for example, increase or decrease activity (e.g., increase or decrease the ability of the peptide to stimulate intestinal motility), alter the ability of the peptide to fold correctly, the stability of the peptide, the ability of the peptide to bind the GC—C receptor and/or decrease toxicity. In some cases the peptides may function more desirably than wild-type ST peptide. For example, they may limit undesirable side effects such as diarrhea and dehydration.

In some embodiments one or both members of one or more pairs of Cys residues which normally form a disulfide bond can be replaced by homocysteine, 3-mercaptoproline (Kolodziej et al. 1996 Int J Pept Protein Res 48:274); β, β dimethylcysteine (Hunt et al. 1993 Int J Pept Protein Res 42:249) or diaminopropionic acid (Smith et al. 1978 J Med Chem 21:117) to form alternative internal cross-links at the positions of the normal disulfide bonds.

In addition, one or more disulfide bonds can be replaced by alternative covalent cross-links, e.g., an amide bond, an ester linkage, an alkyl linkage, a thio ester linkage, a lactam bridge, a carbamoyl linkage, a urea linkage, a thiourea linkage, a phosphonate ester linkage, an alkyl linkage, and alkenyl linkage, an ether, a thioether linkage, or an amino linkage. For example, Ledu et al. (Proceedings Nat'l Acad. Sci. 100:11263-78, 2003) described methods for preparing lactam and amide cross-links. Schafmneister et al. (J. Am. Chem. Soc. 122:5891, 2000) describes stable, all carbon cross-links. In some cases, the generation of such alternative cross-links requires replacing the Cys residues with other residues such as Lys or Glu or non-naturally occurring amino acids.

In the case of a peptide comprising the sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:119)wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is missing and/or the sequence Xaa₁₉Xaa₂₀Xaa₂₁is missing, the peptide can still contain additional carboxyterminal or amino terminal amino acids or both. For example, the peptide can include an amino terminal sequence that facilitates recombinant production of the peptide and is cleaved prior to administration of the peptide to a patient. The peptide can also include other amino terminal or carboxyterminal amino acids. In some cases the additional amino acids protect the peptide, stabilize the peptide or alter the activity of the peptide. In some cases some or all of these additional amino acids are removed prior to administration of the peptide to a patient. The peptide can include 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 40, 50, 60, 70 80, 90, 100 or more amino acids at its amino terminus or carboxy terminus or both. The number of flanking amino acids need not be the same. For example, there can be 10 additional amino acids at the amino terminus of the peptide and none at the carboxy terminus.

In one embodiment the peptide comprises the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is missing; Xaa₈is Glu; Xaa₉is Leu, Ile, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn; Xaa₁₃is Pro; Xaa₁₄is Ala; Xaa₁₆is Thr, Ala, Lys, Arg, Trp; Xaa₁₇is Gly; Xaa₁₉is Tyr or Leu; and Xaa₂₀Xaa₂₁is AspPhe or is missing. Where Xaa₂₀Xaa₂₁and/or Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅are missing, there may be additional flanking amino acids in some embodiments.

In a second aspect, the invention also features a therapeutic or prophylactic method comprising administering a peptide comprising the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is Asn Ser Ser Asn Tyr (SEQ ID NO:121) or is missing or Xaa₁Xaa₂Xaa₃Xaa₄is missing and Xaa₅is Asn, Trp, Tyr, Asp, Ile, Thr, or Phe; Xaa₈is Glu, Asp, Gln, Gly or Pro; Xaa₉is Leu, Ile, Val, Ala, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn, Tyr, Asp or Ala; Xaa₁₃is Pro or Gly; Xaa₁₄is Ala, Leu, Ser, Gly, Val, Glu, Gln, Ile, Leu, Lys, Arg, and Asp; Xaa₁₆is Thr, Ala, Asn, Lys, Arg, Trp; Xaa₁₇is Gly, Pro or Ala; Xaa₁₉is Trp, Tyr, Phe or Leu; and Xaa₂₀Xaa₂₁is AspPhe or is missing or Xaa₂₀is Asn or Glu and Xaa₂₁is missing or Xaa₁₉Xaa₂₀Xaa₂₁is missing.

In certain embodiments of the therapeutic or prophylactic methods: the peptide comprises the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is missing; Xaa₈is Glu; Xaa₉is Leu, Ile, Lys, Arg, Trp, Tyr, or Phe; Xaa₁₂is Asn; Xaa₁₃is Pro; Xaa₁₄is Ala; Xaa₁₆is Thr, Ala, Lys, Arg, Trp or Xaa₁₆is any amino acid or Xaa₁₆is Thr, Ala, Lys, Arg, Trp or Xaa₁₆is any non-aromatic amino acid; Xaa₁₇is Gly; Xaa₁₉is Tyr or Leu; and Xaa₂₀Xaa₂₁is AspPhe or is missing.

In certain embodiments, the invention features, a purified polypeptide comprising the amino acid sequence (II):

Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Asn₁₂Pro₁₃Ala₁₄Cys₁₅Xaa₁₆Gly₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:120) wherein

Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅is Asn Ser Ser Asn Tyr (SEQ ID NO:121) or is missing or Xaa₁Xaa₂Xaa₃Xaa₄is missing and Xaa₅is Asn;

Xaa ₈is Glu or Asp;

Xaa ₉is Leu, Ile, Val, Trp, Tyr or Phe;

Xaa ₁₆is Thr, Ala, Trp;

Xaa ₁₉is Trp, Tyr, Phe or Leu or is missing; and Xaa₂₀Xaa₂₁is AspPhe.

In various preferred embodiments the invention features a purified polypeptide comprising the amino acid sequence (II): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Asn₁₂Pro₁₃Ala₁₄Cys₁₅Xaa₁₆Gly₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:120) wherein, Xaa₉is Leu, Ile or Val and Xaa₁₆is Trp, Tyr or Phe; Xaa₉is Trp, Tyr or Phe, and Xaa₁₆is Thr or Ala; Xaa₁₉is Trp, Tyr, Phe and Xaa₂₀Xaa₂₁is AspPhe; and Xaa₁Xaa₂Xaa₃Xaa₄is missing and Xaa₅is Asn; the peptide comprises fewer than 50, 40, 30 or 25 amino acids; fewer than five amino acid precede Cys₆.

The peptides can be co-administered with or linked, e.g., covalently linked to any of a variety of other peptides including analgesic peptides or analgesic compounds. For example, a therapeutic peptide of the invention can be linked to an analgesic agent selected from the group consisting of: Ca channel blockers (e.g., ziconotide), complete or partial 5HT receptor antagonists (for example 5HT3, 5HT4 and 5HT1 receptor antagonists), complete or partial 5HT receptor agonists including 5HT3, 5HT4 (for example tegaserod, mosapride and renzapride) and 5HT1 receptor agonists, CRF receptor agonists (NBI-34041), β-3 adrenoreceptor agonists, opioid receptor agonists (e.g., loperamide, fedotozine, and fentanyl, naloxone, naltrexone, methyl nalozone, nalmefene, cypridime, beta funaltrexamine, naloxonazine, naltrindole, and nor-binaltorphimine, morphine, diphenyloxylate, enkephalin pentapeptide, asimadoline, and trimebutine), NK1 receptor antagonists (e.g., ezlopitant and SR-14033), CCK receptor agonists (e.g., loxiglumide), NK1 receptor antagonists, NK3 receptor antagonists (e.g., talnetant, osanetant (SR-142801)), norepinephrine-serotonin reuptake inhibitors (NSRI; e.g., milnacipran), vanilloid and cannabanoid receptor agonists (e.g., arvanil), sialorphin, sialorphin-related peptides comprising the amino acid sequence QHNPR (SEQ ID NO:111) for example, VQHNPR (SEQ ID NO:112); VRQHNPR (SEQ ID NO:113); VRGQHNPR (SEQ ID NO:114); VRGPQHNPR (SEQ ID NO:115); VRGPRQHNPR (SEQ ID NO:116); VRGPRRQHNPR (SEQ ID NO:117); and RQHNPR (SEQ ID NO:118), compounds or peptides that are inhibitors of neprilysin, frakefamide (H-Tyr-D-Ala-Phe(F)-Phe-NH ₂; WO 01/019849 A1), loperamide, Tyr-Arg (kyotorphin), CCK receptor agonists (caerulein), conotoxin peptides, pepetide analogs of thymulin, loxiglumide, dexloxiglumide (the R-isomer of loxiglumide) (WO 88/05774) and other analgesic peptides or compounds can be used with or linked to the peptides of the invention.

Amino acid, non-amino acid, peptide and non-peptide spacers can be interposed between a peptide that is a GC—C receptor agonsit and a peptide that has some other biological function, e.g., an analgesic peptide or a peptide used to treat obesity. The linker can be one that is cleaved from the flanking peptides in vivo or one that remains linked to the flanking peptides in vivo. For example, glycine, beta-alanine, glycyl-glycine, glycyl-beta-alanine, gamma-aminobutyric acid, 6-aminocaproic acid, L-phenylalanine, L-tryptophan and glycil-L-valil-L-phenylalanine can be used as a spacer (Chaltin et al. 2003 Helvetica Chimica Acta 86:533-547; Caliceti et al. 1993 FARMCO 48:919-32) as can polyethylene glycols (Butterworth et al. 1987 J. Med. Chem 30:1295-302) and maleimide derivatives (King et al. 2002 Tetrahedron Lett. 43:1987-1990). Various other linkers are described in the literature (Nestler 1996 Molecular Diversity 2:35-42; Finn et al. 1984 Biochemistry 23:2554-8; Cook et al. 1994 Tetrahedron Lett. 35:6777-80; Brokx et al. 2002 Journal of Controlled Release 78:115-123; Griffin et al. 2003 J. Am. Chem. Soc. 125:6517-6531; Robinson et al. 1998 Proc. Natl. Acad. Sci. USA 95:5929-5934.

The peptides can include the amino acid sequence of a peptide that occurs naturally in a vertebrate (e.g., mammalian) species or in a bacterial species. In addition, the peptides can be partially or completely non-naturally occurring peptides. Also within the invention are peptidomimetics corresponding to the peptides of the invention. In various embodiments, the patient is suffering from a gastrointestinal disorder; the patient is suffering from a disorder selected from the group consisting of: a gastrointestinal motility disorder, irritable bowel syndrome, chronic constipation, a functional gastrointestinal disorder, gastroesophageal reflux disease, functional heartburn, dyspepsia, functional dyspepsia, nonulcer dyspepsia, gastroparesis, chronic intestinal pseudo-obstruction, Crohn's disease, ulcerative colitis, Irritable bowel syndrome, colonic pseudo-obstruction, obesity, congestive heart failure, or benign prostatic hyperplasia; the composition is administered orally; the peptide comprises 30 or fewer amino acids, the peptide comprises 20 or fewer amino acids, and the peptide comprises no more than 5 amino acids prior to Cys ₆; the peptide comprises 150, 140, 130, 120, 110, 100, 90, 80, 70, 60, 50, 40, or 30 or fewer amino acids. In other embodiments, the peptide comprises 20 or fewer amino acids. In other embodiments the peptide comprises no more than 20, 15, 10, or 5 peptides subsequent to Cys₁₈. In certain embodiments Xaa₁₉is a chymotrypsin or trypsin cleavage site and an analgesic peptide is present immediately following Xaa₁₉.

In a third aspect, the invention features a method for treating a patient suffering from constipation. Clinically accepted criteria that define constipation range from the frequency of bowel movements, the consistency of feces and the ease of bowel movement. One common definition of constipation is less than three bowel movements per week. Other definitions include abnormally hard stools or defecation that requires excessive straining (Schiller 2001, Aliment Pharmacol Ther 15:749-763). Constipation may be idiopathic (functional constipation or slow transit constipation) or secondary to other causes including neurologic, metabolic or endocrine disorders. These disorders include diabetes mellitus, hypothyroidism, hyperthyroidism, hypocalcaemia, Multiple Sclerosis, Parkinson's disease, spinal cord lesions, Neurofibromatosis, autonomic neuropathy, Chagas disease, Hirschsprung's disease and Cystic fibrosis. Constipation may also be the result of surgery (postoperative ileus) or due to the use of drugs such as analgesics (like opiods), antihypertensives, anticonvulsants, antidepressants, antispasmodics and antipsychotics.

The method comprising administering a composition comprising a purified polypeptide comprising the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is Asn Ser Ser Asn Tyr (SEQ ID NO:121) or is missing or Xaa₁Xaa₂Xaa₃Xaa₄is missing and Xaa₅is Asn, Trp, Tyr, Asp, Ile, Thr, or Phe; Xaa₈is Glu, Asp, Gln, Gly or Pro; Xaa₉is Leu, Ile, Val, Ala, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn, Tyr, Asp or Ala; Xaa₁₃is Pro or Gly; Xaa₁₄is Ala, Leu, Ser, Gly, Val, Glu, Gln, Ile, Leu, Lys, Arg, and Asp; Xaa₁₆is Thr, Ala, Asn, Lys, Arg, Trp; Xaa₁₇is Gly, Pro or Ala; Xaa₁₉is Trp, Tyr, Phe or Leu; Xaa₁₉is Lys or Arg; Xaa₂₀Xaa₂₁is AspPhe or is missing or Xaa₂₀is Asn or Glu and Xaa₂₁is missing or Xaa₁₉Xaa₂₀Xaa₂₁is missing.

In one embodiment of the method, the peptide comprises the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is missing; Xaa₈is Glu; Xaa₉is Leu, Ile, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn; Xaa₁₃is Pro; Xaa₁₄is Ala; Xaa₁₆is Thr, Ala, Lys, Arg, Trp; Xaa₁₇is Gly; Xaa₁₉is Tyr or Leu; Xaa₁₉is Lys or Arg; Xaa₂₀Xaa₂₁is AspPhe or is missing.

In various preferred embodiments, the constipation is associated with use of a therapeutic agent; the constipation is associated with a neuropathic disorder; the constipation is post-surgical constipation (postoperative ileus); and the constipation associated with a gastrointestinal disorder; the constipation is idiopathic (functional constipation or slow transit constipation); the constipation is associated with neuropathic, metabolic or endocrine disorder (e.g., diabetes mellitus, hypothyroidism, hyperthyroidism, hypocalcaemia, Multiple Sclerosis, Parkinson's disease, spinal cord lesions, neurofibromatosis, autonomic neuropathy, Chagas disease, Hirschsprung's disease or cystic fibrosis). Constipation may also be the result of surgery (postoperative ileus) or due the use of drugs such as analgesics (e.g., opiods), antihypertensives, anticonvulsants, antidepressants, antispasmodics and antipsychotics.

In a fourth aspect, the invention features a method for treating a patient suffering a gastrointestinal disorder, the method comprising administering to the patient a composition comprising a purified polypeptide comprising the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is Asn Ser Ser Asn Tyr (SEQ ID NO:121) or is missing or Xaa₁Xaa₂Xaa₃Xaa₄is missing and Xaa₅is Asn, Trp, Tyr, Asp, Ile, Thr, or Phe; Xaa₈is Glu, Asp, Gln, Gly or Pro; Xaa₉is Leu, Ile, Val, Ala, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn, Tyr, Asp or Ala; Xaa₁₃is Pro or Gly; Xaa₁₄is Ala, Leu, Ser, Gly, Val, Glu, Gln, Ile, Leu, Lys, Arg, and Asp; Xaa₁₆is Thr, Ala, Asn, Lys, Arg, Trp; Xaa₁₇is Gly, Pro or Ala; Xaa₁₉is Trp, Tyr, Phe or Leu; Xaa₁₉is Lys or Arg;Xaa₂₀Xaa₂₁is AspPhe or is missing or Xaa₂₀is Asn or Glu and Xaa₂₁is missing or Xaa₁₉Xaa₂₀Xaa₂₁is missing.

In various embodiments, the patient is suffering from a gastrointestinal disorder; the patient is suffering from a disorder selected from the group consisting of: a gastrointestinal motility disorder, irritable bowel syndrome, chronic constipation, a functional gastrointestinal disorder, gastroesophageal reflux disease, functional heartburn, dyspepsia, functional dyspepsia, nonulcer dyspepsia, gastroparesis, chronic intestinal pseudo-obstruction, Crohn's disease, ulcerative colitis, Inflammatory bowel disease, colonic pseudo-obstruction, obesity, congestive heart failure, or benign prostatic hyperplasia.

In various preferred embodiments, Xaa ₉is Leu, Ile or Val and Xaa₁₆is Trp, Tyr or Phe; Xaa₉is Trp, Tyr or Phe and Xaa₁₆is Thr or Ala; Xaa₁₉is Trp, Tyr, Phe; Xaa₁₉is Lys or Arg;Xaa₂₀Xaa₂₁is AspPhe; Xaa₁Xaa₂Xaa₃Xaa₄is missing and Xaa₅is Asn.

In a fifth aspect, the invention features a method for increasing gastrointestinal motility in a patient, the method comprising: administering to the patient a composition comprising a purified polypeptide comprising the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is Asn Ser Ser Asn Tyr (SEQ ID NO:121) or is missing or Xaa₁Xaa₂Xaa₃Xaa₄is missing and Xaa₅is Asn, Trp, Tyr, Asp, Ile, Thr, or Phe; Xaa₈is Glu, Asp, Gln, Gly or Pro; Xaa₉is Leu, Ile, Val, Ala, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn, Tyr, Asp or Ala; Xaa₁₃is Pro or Gly; Xaa₁₄is Ala, Leu, Ser, Gly, Val, Glu, Gln, Ile, Leu, Lys, Arg, and Asp; Xaa₁₆is Thr, Ala, Asn, Lys, Arg, Trp; Xaa₁₇is Gly, Pro or Ala; Xaa₁₉is Trp, Tyr, Phe or Leu; Xaa₁₉is Lys or Arg; Xaa₂₀Xaa₂₁is AspPhe or is missing or Xaa₂₀is Asn or Glu and Xaa₂₁is missing or Xaa₁₉Xaa₂₀Xaa₂₁is missing.

In one embodiment the peptide comprises the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is missing; Xaa₈is Glu; Xaa₉is Leu, Ile, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn; Xaa₁₃is Pro; Xaa₁₄is Ala; Xaa₁₆is Thr, Ala, Lys, Arg, Trp; Xaa₁₇is Gly; Xaa₁₉is Tyr or Leu; Xaa₁₉is Lys or Arg; Xaa₂₀Xaa₂₁is AspPhe or is missing.

In a sixth aspect, the invention features a method for increasing the activity of an intestinal guanylate cyclase (GC—C) receptor in a patient, the method comprising: administering to the patient a composition comprising a purified polypeptide comprising the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is Asn Ser Ser Asn Tyr (SEQ ID NO:121) or is missing or Xaa₁Xaa₂Xaa₃Xaa₄is missing and Xaa₅is Asn, Trp, Tyr, Asp, Ile, Thr, or Phe; Xaa₈is Glu, Asp, Gln, Gly or Pro; Xaa₉is Leu, Ile, Val, Ala, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn, Tyr, Asp or Ala; Xaa₁₃is Pro or Gly; Xaa₁₄is Ala, Leu, Ser, Gly, Val, Glu, Gln, Ile, Leu, Lys, Arg, and Asp; Xaa₁₆is Thr, Ala, Asn, Lys, Arg, Trp; Xaa₁₇is Gly, Pro or Ala; Xaa₁₉is Trp, Tyr, Phe or Leu; Xaa₁₉is Lys or Arg; Xaa₂₀Xaa₂₁is AspPhe or is missing or Xaa₂₀is Asn or Glu and Xaa₂₁is missing or Xaa₁₉Xaa₂₀Xaa₂₁is missing.

In a seventh aspect, the invention features an isolated nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide comprising the amino acid sequence: (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is Asn Ser Ser Asn Tyr or is missing or Xaa₁Xaa₂Xaa₃Xaa₄is missing and Xaa₅is Asn, Trp, Tyr, Asp, Ile, Thr, or Phe; Xaa₈is Glu, Asp, Gln, Gly or Pro; Xaa₉is Leu, Ile, Val, Ala, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn, Tyr, Asp or Ala; Xaa₁₃is Pro or Gly; Xaa₁₄is Ala, Leu, Ser, Gly, Val, Glu, Gln, Ile, Leu, Lys, Arg, and Asp; Xaa₁₆is Thr, Ala, Asn, Lys, Arg, Trp; Xaa₁₇is Gly, Pro or Ala; Xaa₁₉is Trp, Tyr, Phe or Leu; Xaa₁₉is Lys or Arg; Xaa₂₀Xaa₂₁is AspPhe or is missing or Xaa₂₀is Asn or Glu and Xaa₂₁is missing or Xaa₁₉Xaa₂₀Xaa₂₁is missing.

In an eighth aspect the invention features a method for treating constipation, the method comprising administering an agonist of the intestinal guanylate cyclase (GC—C) receptor. In various embodiments: the agonist is a peptide, the peptide includes four Cys that form two disulfide bonds, and the peptide includes six Cys that form three disulfide bonds.

In a ninth aspect, the invention features a method for treating a gastrointestinal disorder, a gastrointestinal motility disorder, irritable bowel syndrome, chronic constipation, a functional gastrointestinal disorder, gastroesophageal reflux disease, functional heartburn, dyspepsia, functional dyspepsia, nonulcer dyspepsia, gastroparesis, chronic intestinal pseudo-obstruction, colonic pseudo-obstruction, Crohn's disease, ulcerative colitis, Inflammatory bowel disease, obesity, congestive heart failure, or benign prostatic hyperplasia, the method comprising administering an agonist of the intestinal guanylate cyclase (GC—C) receptor either orally, by rectal suppository, or parenterally. In various embodiments: the agonist is a peptide, the peptide includes four Cys that form two disulfide bonds, and the peptide includes six Cys that form three disulfide bonds.

In a tenth aspect, the invention features a method for treating a gastrointestinal disorder selected from the group consisting of: a gastrointestinal motility disorder, irritable bowel syndrome, chronic constipation, a functional gastrointestinal disorder, gastroesophageal reflux disease, functional heartburn, dyspepsia, functional dyspepsia, nonulcer dyspepsia, gastroparesis, chronic intestinal pseudo-obstruction, colonic pseudo-obstruction, Crohn's disease, ulcerative colitis, Inflammatory bowel disease, the method comprising administering an agonist of the intestinal guanylate cyclase (GC—C) receptor. In various embodiments the composition is administered orally; the peptide comprises 30 or fewer amino acids, the peptide comprises 20 or fewer amino acids, and the peptide comprises no more than 5 amino acids prior to Cys ₅.

In various embodiments: the agonist is a peptide, the peptide includes four Cys that form two disulfide bonds, and the peptide includes six Cys that form three disulfide bonds.

In an eleventh aspect, the invention features a method for treating obesity, the method comprising administering an agonist of the intestinal guanylate cyclase (GC—C) receptor. In various embodiments: the agonist is a peptide, the peptide includes four Cys that form two disulfide bonds, and the peptide includes six Cys that form three disulfide bonds.

In a twelfth aspect, the invention features a method for treating obesity, the method comprising administering a polypeptide comprising the amino acid sequence: (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is Asn Ser Ser Asn Tyr (SEQ ID NO:121) or is missing or Xaa₁Xaa₂Xaa₃Xaa₄is missing and Xaa₅is Asn, Trp, Tyr, Asp, Ile, Thr, or Phe; Xaa₈is Glu, Asp, Gln, Gly or Pro; Xaa₉is Leu, Ile, Val, Ala, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn, Tyr, Asp or Ala; Xaa₁₃is Pro or Gly; Xaa₄is Ala, Leu, Ser, Gly, Val, Glu, Gln, Ile, Leu, Lys, Arg, and Asp; Xaa₁₆is Thr, Ala, Asn, Lys, Arg, Trp; Xaa₁₇is Gly, Pro or Ala; Xaa₁₉is Trp, Tyr, Phe or Leu; and Xaa₂₀Xaa₂₁is AspPhe or is missing or Xaa₂₀is Asn or Glu and Xaa₂₁is missing or Xaa₁₉Xaa₂₀Xaa₂₁is missing. The peptide can be administered alone or in combination with another agent for the treatment of obesity, e.g., sibutramine or another agent, e.g., an agent described herein.

In one embodiment the peptide comprises the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is missing; Xaa₈is Glu; Xaa₉is Leu, Ile, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn; Xaa₁₃is Pro; Xaa₁₄is Ala; Xaa₁₆is Thr, Ala, Lys, Arg, Trp; Xaa₁₇is Gly; Xaa₁₉is Tyr or Leu; and Xaa₂₀Xaa₂₁is AspPhe or is missing.

In a thirteenth aspect, the invention features a pharmaceutical composition comprising a polypeptide described herein.

In a fourteenth aspect, the invention features a method for treating congestive heart failure, the method comprising: administering to the patient a composition comprising a purified polypeptide comprising the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID NO:119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is Asn Ser Ser Asn Tyr (SEQ ID NO:121) or is missing or Xaa₁Xaa₂Xaa₃Xaa₄is missing and Xaa₅is Asn, Trp, Tyr, Asp, Ile, Thr, or Phe; Xaa₈is Glu, Asp, Gln, Gly or Pro; Xaa₉is Leu, Ile, Val, Ala, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn, Tyr, Asp or Ala; Xaa₁₃is Pro or Gly; Xaa₁₄is Ala, Leu, Ser, Gly, Val, Glu, Gln, Ile, Leu, Lys, Arg, and Asp; Xaa₁₆is Thr, Ala, Asn, Lys, Arg, Trp; Xaa₁₇is Gly, Pro or Ala; Xaa₁₉is Trp, Tyr, Phe or Leu; and Xaa₂₀Xaa₂₁is AspPhe or is missing or Xaa₂₀is Asn or Glu and Xaa₂₁is missing or Xaa₁₉Xaa₂₀Xaa₂₁is missing. The peptide can be administered in combination with another agent for treatment of congestive heart failure, for example, a natriuretic peptide such as atrial natriuretic peptide, brain natriuretic peptide or C-type natriuretic peptide), a diuretic, or an inhibitor of angiotensin converting enzyme.

In one embodiment the peptide comprises the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID No: 119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is missing; Xaa₈is Glu; Xaa₉is Leu, Ile, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn; Xaa₁₃is Pro; Xaa₁₄is Ala; Xaa₁₆is Thr, Ala, Lys, Arg, Trp; Xaa₁₇is Gly; Xaa₁₉is Tyr or Leu; Xaa₁₉is Lys or Arg; Xaa₂₀Xaa₂₁is AspPhe or is missing.

In a fifteenth aspect, the invention features a method for treating benign prostatic hyperplasia, the method comprising: administering to the patient a composition comprising a purified polypeptide comprising the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID No:119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is Asn Ser Ser Asn Tyr (SEQ ID NO:121) or is missing or Xaa₁Xaa₂Xaa₃Xaa₄is missing and Xaa₅is Asn, Trp, Tyr, Asp, Ile, Thr, or Phe; Xaa₈is Glu, Asp, Gln, Gly or Pro; Xaa₉is Leu, Ile, Val, Ala, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn, Tyr, Asp or Ala; Xaa₁₃is Pro or Gly; Xaa₁₄is Ala, Leu, Ser, Gly, Val, Glu, Gln, Ile, Leu, Lys, Arg, and Asp; Xaa₁₆is Thr, Ala, Asn, Lys, Arg, Trp; Xaa₁₇is Gly, Pro or Ala; Xaa₁₉is Trp, Tyr, Phe or Leu; Xaa₁₉is Lys or Arg; Xaa₂₀Xaa₂₁is AspPhe or is missing or Xaa₂₀is Asn or Glu and Xaa₂₁is missing or Xaa₁₉Xaa₂₀Xaa₂₁is missing.

The peptide can be administered in combination with another agent for treatment of BPH, for example, a 5-alpha reductase inhibitor (e.g., finasteride) or an alpha adrenergic inhibitor (e.g., doxazosine).

In one embodiment the peptide comprises the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID No: 119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is missing; Xaa₈is Glu; Xaa₉is Leu, Ile, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn; Xaa₁₃is Pro; Xaa₁₄is Ala; Xaa₁₆is Thr, Ala, Lys, Arg, Trp; Xaa₁₇is Gly; Xaa₁₉is Tyr or Leu; and Xaa₂₀Xaa₂₁is AspPhe or is missing.

In a sixteenth aspect, the invention features a method for treating or reducing pain, including visceral pain, pain associated with a gastrointestinal disorder or pain associated with some other disorder, the method comprising: administering to a patient a composition comprising a is purified polypeptide comprising the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁, (SEQ ID No: 119) e.g., a purified polypeptide comprising an amino acid sequence disclosed herein.

In a seventeenth aspect, the invention features a method for treating inflammation, including inflammation of the gastrointestinal tract, e.g., inflammation associated with a gastrointestinal disorder or infection or some other disorder, the method comprising: administering to a patient a composition comprising a purified polypeptide comprising the amino acid sequence (I): Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁, (SEQ ID No: 119) e.g., a purified polypeptide comprising an amino acid sequence disclosed herein.

In certain embodiments the peptide includes a peptide comprising or consisting of the amino acid sequence Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys Cys Glu Xaa₉Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Xaa₂₀Xaa₂₁(II) (SEQ ID NO:66) wherein Xaa₉is any amino acid, wherein Xaa₉is any amino acid other than Leu, wherein Xaa₉is selected from Phe, Trp and Tyr; wherein Xaa₉is selected from any other natural or non-natural aromatic amino acid, wherein Xaa₉is Tyr; wherein Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is Asn Ser Ser Asn Tyr (SEQ ID NO:121); wherein Xaa₁, Xaa₂, Xaa₃, Xaa₄, and Xaa₅are missing; wherein Xaa₁, Xaa₂, Xaa₃and Xaa₄are missing; wherein Xaa₁, Xaa₂and Xaa₃are missing; wherein Xaa₁and Xaa₂are missing; wherein Xaa₁is missing; wherein Xaa₂₀Xaa₂₁is AspPhe or is missing or Xaa₂₀is Asn or Glu and Xaa₂₁is missing or Xaa₁₉Xaa₂₀Xaa₂₁is missing. In the case of a peptide comprising the sequence (I): Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁₉Xaa₂₀Xaa₂₁(SEQ ID No: 119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is missing and/or the sequence Xaa₁₉Xaa₂₀Xaa₂₁is missing peptide can still contain additional carboxyterminal or amino terminal amino acids or both

Among the useful peptides are peptides comprising, consisting of or consisting essentially of the amino acid sequence Xaa ₁Xaa₂Xaa₃Xaa₄Xaa₅Cys Cys Glu Xaa₉Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Xaa₂₀Xaa₂₁(II) (SEQ ID NO:66) are the following peptides:


Gln Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:67)

Tyr Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Thr Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:68)

Tyr Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Leu Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:69)

Tyr Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ile Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:70)

Tyr Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Gln Tyr Cys Cys Glu	(SEQ ID NO:71)

Tyr Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Ser Ser Asn Tyr Cys Cys Glu Tyr	(SEQ ID NO:72)

Cys Cys Asn Pro Ala Cys Thr Gly

Cys Tyr

Gln Ser Ser Gln Tyr Cys Cys Glu	(SEQ ID NO:73)

Tyr Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Ser Ser Gln Tyr Cys Cys Glu Tyr	(SEQ ID NO:74)

Cys Cys Asn Pro Ala Cys Thr Gly

Cys Tyr.

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:75)

Ala Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:76)

Arg Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:77)

Asn Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:78)

Asp Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:79)

Cys Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:80)

Gln Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:81)

Glu Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:82)

Gly Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:83)

His Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:84)

Ile Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:85)

Lys Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:86)

Met Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:87)

Phe Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:88)

Pro Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:89)

Ser Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:90)

Thr Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:91

Trp Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:92)

Val Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr

Cys Cys Glu Ala Cys Cys Asn Pro	(SEQ ID NO:93)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Arg Cys Cys Asn Pro	(SEQ ID NO:94)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Asn Cys Cys Asn Pro	(SEQ ID NO:95)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Asp Cys Cys Asn Pro	(SEQ ID NO:96)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Cys Cys Cys Asn Pro	(SEQ ID NO:97)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Gln Cys Cys Asn Pro	(SEQ ID NO:98)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Glu Cys Cys Asn Pro	(SEQ ID NO:99)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Gly Cys Cys Asn Pro	(SEQ ID NO:100)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu His Cys Cys Asn Pro	(SEQ ID NO:101)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Ile Cys Cys Asn Pro	(SEQ ID NO:102)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Lys Cys Cys Asn Pro	(SEQ ID NO:103)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Met Cys Cys Asn Pro	(SEQ ID NO:104)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Phe Cys Cys Asn Pro	(SEQ ID NO:105)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Pro Cys Cys Asn Pro	(SEQ ID NO:106)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Ser Cys Cys Asn Pro	(SEQ ID NO:107)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Thr Cys Cys Asn Pro	(SEQ ID NO:108)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Trp Cys Cys Asn Pro	(SEQ ID NO:109)

Ala Cys Thr Gly Cys Tyr

Cys Cys Glu Val Cys Cys Asn Pro	(SEQ ID NO:110)

Ala Cys Thr Gly Cys Tyr

In an eighteenth aspect, the invention features a method for treating congestive heart failure, the method comprising administering a complete or partial agonist of the intestinal guanylate cyclase (GC—C) receptor. The agonist can be administered in combination with another agent for treatment of congestive heart failure, for example, a natriuretic peptide such as atrial natriuretic peptide, brain natriuretic peptide or C-type natriuretic peptide), a diuretic, or an inhibitor of angiotensin converting enzyme.

In a nineteenth aspect, the invention features a method for treating BPH, the method comprising administering a complete or partial agonist of the intestinal guanylate cyclase (GC—C) receptor. The agonist can be administered in combination with another agent for treatment of BPH, for example, a 5-alpha reductase inhibitor (e.g., finasteride) or an alpha adrenergic inhibitor (e.g., doxazosine).

In a twentieth aspect, the invention features a method for treating obesity, the method comprising administering a complete or partial agonist of the intestinal guanylate cyclase (GC—C) receptor. The agonist can be administered in combination with another agent for treatment of obesity, for example, gut hormone fragment peptide YY _3-36(PYY_3-36)(N. Engl. J. Med. 349:941, 2003; ikpeapge daspeelnry yaslrhylnl vtrqry) glp-1 (glucagon-like peptide-1), exendin-4 (an inhibitor of glp-1), sibutramine, phentermine, phendimetrazine, benzphetamine hydrochloride (Didrex), orlistat (Xenical), diethylpropion hydrochloride (Tenuate), fluoxetine (Prozac), bupropion, ephedra, chromium, garcinia cambogia, benzocaine, bladderwrack (focus vesiculosus), chitosan, nomame herba, galega (Goat's Rue, French Lilac), conjugated linoleic acid, L-carnitine, fiber (psyllium, plantago, guar fiber), caffeine, dehydroepiandrosterone, germander (teucrium chamaedrys), B-hydroxy-β-methylbutyrate, and pyruvate. A peptide useful for treating obesity can be administered as a co-therapy with a peptide of the invention either as a distinct molecule or as part of a fusion protein with a peptide of the invention. Thus, for example, PYY_3-36can be fused to the carboxy or amino terminus of a peptide of the invention. Such a fusion protein can include a chymostrypsin or trypsin cleavage site that can permit cleavage to separate the two peptides.

The peptides and agonist of the intestinal guanylate cyclase (GC—C) receptor can be used to treat constipation or decreased intestinal motility, slow digestion or slow stomach emptying. The peptides can be used to relieve one or more symptoms of IBS (bloating, pain, constipation), GERD (acid reflux into the esophagus), functional dyspepsia, or gastroparesis (nausea, vomiting, bloating, delayed gastric emptying) and other disorders described herein.

The details of one or more embodiments of the invention are set forth in the accompanying description. All of the publications, patents and patnet applications are hereby incorporated by reference.

FIGURES

FIG. 1[0076] a depicts the results of LCMS analysis of recombinant MM-416776 peptide and MD-915 peptide.
FIGS. 1[0077] b and c depict the results of LCMS analysis of synthetic MD-1100 peptide and the blank.
FIG. 2 depicts the results of the intestinal GC—C receptor activity assay of synthetic MM-416776 peptide, MD-915 peptide and two different MD-1100 peptides. [0078]
FIG. 3[0079] a depicts the effect of recombinant MM-416776 peptide and Zelnorm® in a murine gastrointestinal transit model.
FIG. 3[0080] b depicts the effect of synthetic MD-1100 peptide and Zelnorm® in an acute murine gastrointestinal transit model.
FIG. 3[0081] b depicts the effect of synthetic MD-1100 peptide and Zelnorm® in an chronic murine gastrointestinal transit model.
FIGS. 4[0082] a and 4 b depict the effect of peptides MD-915, MD-1100, and MM-416776 in an acute murine gastrointestinal transit model.
FIG. 4[0083] c depicts the effect of MD-1100 peptide in a chronic murine gastrointestinal transit model.
FIG. 5[0084] a depicts the effect of MM-416776 peptide and Zelnorm® in a suckling mouse intestinal secretion model.
FIG. 5[0085] b depicts the effects of MD-1100 and Zelnorm® in a mouse intestinal secretion model.
FIGS. 6[0086] a and 6 b depict the effects of MM 416776, MD-1100 and MD-915 peptides in a mouse intestinal secretion model.
FIG. 7 shows the results of experiment in which MD-1100 activity was analyzed in the TNBS colonic distention model. [0087]
FIGS. 8[0088] a and 8 b show the effects of differing doses of MD-915 and MD-1100 in the PBQ writhing assay.
FIG. 9 shows the results of Kd determination analysis using MD-1100 in a competitive radioligand binding assay. [0089]
FIGS. 10[0090] a and 10 b show bioavailability data for IV and orally administered MD-1100 as detected by an ELISA assay and LCMS.

DETAILED DESCRIPTION

The peptides of the invention bind to the intestinal guanylate cyclase (GC—C) receptor, a key regulator of fluid and electrolyte balance in the intestine. When stimulated, this receptor, which is located on the apical membrane of the intestinal epithelial surface, causes an increase in intestinal epithelial cyclic GMP (cGMP). This increase in cGMP is believed to cause a decrease in water and sodium absorption and an increase in chloride and potassium ion secretion, leading to changes in intestinal fluid and electrolyte transport and increased intestinal motility. The intestinal GC—C receptor possesses an extracellular ligand binding region, a transmembrane region, an intracellular protein kinase-like region and a cyclase catalytic domain. Proposed functions for the GC—C receptor are fluid and electrolyte homeostasis, the regulation of epithelial cell proliferation and the induction of apoptosis (Shalubhai 2002 Curr Opin Drug Dis Devel 5:261-268). [0091]
In addition to being expressed in the intestine by gastrointestinal epithelial cells, GC—C is expressed in extra-intestinal tissues including kidney, lung, pancreas, pituitary, adrenal, developing liver (reviewed in Vaandrager 2002, Mol Cell Biochem 230:73-83) and male and female reproductive tissues (reviewed in Vaandrager 2002 [0092] Mol Cell Biochem 230:73-83)) This suggests that the GC—C receptor agonists can be used in the treatment of disorders outside the GI tract, for example, congestive heart failure and benign prostatic hyperplasia.
Ghrelin, a peptide hormone secreted by the stomach, is a key regulator of appetite in humans. Ghrelin expression levels are regulated by fasting and by gastric emptying (Kim et al., 2003, Neuroreprt 14:1317-20; Gualillo et al., 2003, FEBS Letts 552: 105-9). Thus, by increasing gastrointestinal motility, GC—C receptor agonists may also be used to regulate obesity. [0093]
In humans, the GC—C receptor is activated by guanylin (Gn) (U.S. Pat. No. 5,96,097), uroguanylin (Ugn) (U.S. Pat. No. 5,140,102) and lymphoguanylin (Forte et al., 1999, [0094] Endocrinology 140:1800-1806). Interestingly, these agents are 10-100 fold less potent than a class of bacterially derived peptides, termed ST (reviewed in Gianella 1995 J Lab Clin Med 125:173-181). ST peptides are considered super agonists of GC—C and are very resistant to proteolytic degradation.
ST peptide is capable of stimulating the enteric nervous system (Rolfe et al., 1994, J Physiolo 475: 531-537; Rolfe et al., 1999, Gut 44: 615-619; Nzegwu et al., 1996, Exp Physiol 81: 313-315). Also, cGMP has been reported to have anitnociceptive effects in multiple animal models of pain (Lazaro Ibanez et al., 2001, Eur J Pharmacol 426: 39-44; Soares et al., 2001, British J Pharmacol 134: 127-131; Jain et al., 2001, Brain Res 909:170-178; Amarante et al., 2002, Eur J Pharmacol 454:19-23). Thus, GC—C agonists may have both an analgesic as well an anti-inflammatory effect. [0095]
In bacteria, ST peptides are derived from a preproprotein that generally has at least 70 amino acids. The pre and pro regions are cleaved as part of the secretion process, and the resulting mature protein, which generally includes fewer than 20 amino acids, is biologically active. [0096]

Among the known bacterial ST peptides are: E. coli ST Ib (Moseley et al. (1983) Infect. Immun. 39:1167) having the mature amino acid sequence Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr (SEQ ID NO:1); E. coli ST Ia (So and McCarthy (1980) Proc. Natl. Acad. Sci. USA 77:4011) having the mature amino acid sequence Asn Thr Phe Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Ala Gly Cys Tyr (SEQ ID NO:2); E. coli ST I* (Chan and Giannella (1981) J. Biol. Chem. 256:7744) having the mature amino acid sequence Asn Thr Phe Tyr Cys Cys Glu Leu Cys Cys Tyr Pro Ala Cys Ala Gly Cys Asn (SEQ ID NO:3); C.freundii ST peptide (Guarino et al. (1989) Infect. Immun. 57:649) having the mature amino acid sequence Asn Thr Phe Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Ala Gly Cys Tyr (SEQ ID NO:4); Y. enterocolitica ST peptides, Y-ST(Y-STa), Y-STb, and Y-STc (reviewed in Huang et al. (1997) Microb. Pathog. 22:89) having the following pro-form amino acid sequences: Gln Ala Cys Asp Pro Pro Ser Pro Pro Ala Glu Val Ser Ser Asp Trp Asp Cys Cys Asp Val Cys Cys Asn Pro Ala Cys Ala Gly Cys (SEQ ID NO:5) (as well as a Ser-7 to Leu-7 variant of Y-STa (SEQ ID NO:122), (Takao et al. (1985) Eur. J. Biochem. 152:199)); Lys Ala Cys Asp Thr Gln Thr Pro Ser Pro Ser Glu Glu Asn Asp Asp Trp Cys Cys Glu Val Cys Cys Asn Pro Ala Cys Ala Gly Cys (SEQ ID NO:6); Gln Glu Thr Ala Ser Gly Gln Val Gly Asp Val Ser Ser Ser Thr Ile Ala Thr Glu Val Ser Glu Ala Glu Cys Gly Thr Gln Ser Ala Thr Thr Gln Gly Glu Asn Asp Trp Asp Trp Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Phe Gly Cys (SEQ ID NO:7), respectively; Y. kristensenii ST peptide having the mature amino acid sequence Ser Asp Trp Cys Cys Glu Val Cys Cys Asn Pro Ala Cys Ala Gly Cys (SEQ ID NO:8); V. cholerae non-01 ST peptide (Takao et al. (1985) FEBS lett. 193:250) having the mature amino acid sequence Ile Asp Cys Cys Glu Ile Cys Cys Asn Pro Ala Cys Phe Gly Cys Leu Asn (SEQ ID NO:9); and V. mimicus ST peptide (Arita (1991) et al. FEMS Microbiol. Lett. 79:105) having the mature amino acid sequence Ile Asp Cys Cys Glu Ile Cys Cys Asn Pro Ala Cys Phe Gly Cys Leu Asn (SEQ ID NO:10). The Table below provides sequences of all or a portion of a number of mature ST peptides.



Gen-
Bank ®	Gen-
Acces-	Bank ®
sion	GI	Sequence

QHECIB	69638	NSSNYCCELCCNPACTGCY	(SEQ ID NO:1)
P01559	123711	NTFYCCELCCNPACAGCY	(SEQ ID NO:2)

AAA24653	147878	NTFYCCELCCNPACAPCY	(SEQ ID NO:11)

P01560	123707	NTFYCCELCCYPACAGCN	(SEQ ID NO:3)

AAA27561	295439	IDCCEICCNPACFGCLN	(SEQ ID NO:9)

P04429	123712	IDCCEICCNPACFGCLN	(SEQ ID NO:10)

S34671	421286	IDCCEICCNPACF	(SEQ ID NO:12)

CAA52209	395161	IDCCEICCNPACFG	(SEQ ID NO:13)

A54534	628844	IDCCEICCNPACFGCLN	(SEQ ID NO:14)

AAL02159	15592919	IDRCEICCNPACFGCLN	(SEQ ID NO:15)

AAA18472	487395	DWDCCDVCCNPACAGC	(SEQ ID NO:16)

S25659	282047	DWDCCDVCCNPACAGC	(SEQ ID NO:17)

P74977	3913874	NDDWCCEVCCNPACAGC	(SEQ ID NO:18)

BAA23656	2662339	WDWCCELCCNPACFGC	(SEQ ID NO:19)

P31518	399947	SDWCCEVCCNPACAGC	(SEQ ID NO:8)

The immature (including pre and pro regions) form of [0098] E. coli ST-1A (ST-P) protein has the sequence: mkklmlaifisvlsfpsfsqstesldsskekitletkkcdvvknnsekksenmnntfyccelccnpacagcy (SEQ ID NO:20; see GenBank® Accession No. P01559 (gi:123711). The pre sequence extends from aa 1-19. The pro sequence extends from aa 20-54. The mature protein extends from 55-72. The immature (including pre and pro regions) form of E. coli ST-1B (ST-H) protein has the sequence: mkksilfiflsvlsfspfaqdakpvesskekitleskkcniakksnksgpesmnssnyccelccnpactgcy (SEQ ID NO: 21; see GenBank® Accession No. P07965 (gi:3915589). The immature (including pre and pro regions) form of Y. enterocolitica ST protein has the sequence: mkkivfvlvlmlssfgafgqetvsgqfsdalstpitaevykqacdpplppaevssdwdccdvccnpacagc (SEQ ID NO:22) see GenBank® Accession No. S25659 (gi:282047).
The peptides of the invention, like the bacterial ST peptides, have six Cys residues. These six Cys residues form three disulfide bonds in the mature and active form of the peptide. If the six Cys residues are identified, from the amino to carboxy terminus of the peptide, as A, B, C, D, E, and F, then the disulfide bonds form as follows: A-D, B-E, and C—F. The formation of these bonds is thought to be important for GC—C receptor binding. Certain of the peptides of the invention include a potentially functional chymotrypsin cleavage site, e.g., a Trp, Tyr or Phe located between either Cys B and Cys D or between Cys E and Cys F. Cleavage at either chymotrypsin cleavage site reduces or eliminates the ability of the peptide to bind to the GC—C receptor. [0099]
In the human body an inactive form of chymotrypsin, chymotrypsinogen is produced in the pancreas. When this inactive enzyme reaches the small intestine it is converted to active chymotrypsin by the excision of two di-peptides. Active chymotrypsin can potentially cleave peptides at the peptide bond on the carboxy-terminal side of Trp, Tyr or Phe. The presence of active chymotrypsin in the intestinal tract can potentially lead to cleavage of certain of the peptides of the invention having an appropriately positioned functional chymotrypsin cleavage site. It is expected that chymotrypsin cleavage will moderate the action of a peptide of the invention having an appropriately positioned chymotrypsin cleavage site as the peptide passes through the intestinal tract. [0100]
Trypsinogen, like chymotrypsin, is a serine protease that is produced in the pancreas and is present in the digestive tract. The active form, trypsin, will cleave peptides having a Lys or Arg. The presence of active trypsin in the intestinal tract can lead to cleavage of certain of the peptides of the invention having an appropriately positioned functional trypsin cleavage site. It is expected that chymotrypsin cleavage will moderate the action of a peptide of the invention having an appropriately positioned trypsin cleavage site as the peptide passes through the intestinal tract. [0101]
Many gastrointestinal disorders, including IBS, are associated with abdominal or visceral pain. Certain of the peptides of the invention include analgesic or antinociceptive tags such as the carboxy-terminal sequence AspPhe immediately following a Trp, Tyr or Phe that creates a functional chymotrypsin cleavage site or following Lys or Arg that creates a functional trypsin cleavage site. Chymotrypsin in the intestinal tract can potentially cleave such peptides immediately carboxy terminal to the Trp, Phe or Tyr residue, releasing the dipeptide, AspPhe. This dipeptide has been shown to have analgesic activity in animal models (Abdikkahi et al. 2001, Fundam Clin Pharmacol 15:117-23; Nikfar et al 1997, 29:583-6; Edmundson et al 1998, Clin Pharmacol Ther 63:580-93). In this manner such peptides can treat both pain and inflammation. Other analgesic peptides can be present at the carboxy terminus of the peptide (following a functional cleavage site) including: endomorphin-1, endomorphin-2, nocistatin, dalargin, lupron, and substance P. A number of the useful peptides are based on the core sequence: Cys Cys Glu [0102] Leu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr (SEQ ID NO:29). To create a variant having a potentially functional chymotrypsin cleavage site capable of inactivating the peptide, either the Leu (underlined) or the Thr (underlined) can be replaced by Trp, Phe or Tyr or both the Leu and the Thr can be replaced by (independently) Trp, Phe or Tyr. To create a variant having an analgesic di-peptide, the core sequence is followed by Asp Phe. The carboxy terminal Tyr in the core sequence can allow the Asp Phe dipeptide to be released by chymotrypsin in the digestive tract. The core sequence can be optionally be preceded by Asn Ser Ser Asn Tyr or Asn.

Thus, useful variants based on the core sequence include:


Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:26;

Leu Cys Cys Asn Pro Ala Cys Thr	MM-416776)

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:27)

Leu Cys Cys Asn Pro Ala Cys Trp

Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:28;

Tyr Cys Cys Asn Pro Ala Cys Thr	MD-915)

Gly Cys Tyr

Cys Cys Glu Leu Cys Cys Asn Pro	(SEQ ID NO:29;

Ala Cys Thr Gly Cys Tyr	MM416774)

Cys Cys Glu Leu Cys Cys Asn Pro	(SEQ ID NO:30)

Ala Cys Trp Gly Cys Tyr

Cys Cys Glu Tyr Cys Cys Asn Pro	(SEQ ID NO:31;

Ala Cys Thr Gly Cys Tyr	MD-1100)

Asn Cys Cys Glu Leu Cys Cys Asn	(SEQ ID NO:32)

Pro Ala Cys Thr Gly Cys Tyr

Asn Cys Cys Glu Leu Cys Cys Asn	(SEQ ID NO:33)

Pro Ala Cys Trp Gly Cys Tyr

Asn Cys Cys Glu Phe Cys Cys Asn	(SEQ ID NO:34)

Pro Ala Cys Thr Gly Cys Tyr

Asn Cys Cys Glu Tyr Cys Cys Asn	(SEQ ID NO:35)

Pro Ala Cys Thr Gly Cys Tyr

Asn Cys Cys Glu Trp Cys Cys Asn	(SEQ ID NO:36)

Pro Ala Cys Thr Gly Cys Tyr

Asn Cys Cys Glu Arg Cys Cys Asn	(SEQ ID NO:37)

Pro Ala Cys Thr Gly Cys Tyr

Asn Cys Cys Glu Lys Cys Cys Asn	(SEQ ID NO:38)

Pro Ala Cys Thr Gly Cys Tyr

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:39)

Leu Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr Asp Phe

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:40)

Leu Cys Cys Asn Pro Ala Cys Trp

Gly Cys Tyr Asp Phe

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:41)

Phe Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr Asp Phe

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:42)

Tyr Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr Asp Phe

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:43)

Trp Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr Asp Phe

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:44)

Arg Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr Asp Phe

Asn Ser Ser Asn Tyr Cys Cys Glu	(SEQ ID NO:45)

Lys Cys Cys Asn Pro Ala Cys Thr

Gly Cys Tyr Asp Phe

Cys Cys Glu Leu Cys Cys Asn Pro	(SEQ ID NO:46)

Ala Cys Thr Gly Cys Tyr Asp Phe

Cys Cys Glu Leu Cys Cys Asn Pro	(SEQ ID NO:47)

Ala Cys Trp Gly Cys Tyr Asp Phe

Cys Cys Glu Phe Cys Cys Asn Pro	(SEQ ID NO:48)

Ala Cys Thr Gly Cys Tyr Asp Phe

Cys Cys Glu Tyr Cys Cys Asn Pro	(SEQ ID NO:49)

Ala Cys Thr Gly Cys Tyr Asp Phe

Cys Cys Glu Trp Cys Cys Asn Pro	(SEQ ID NO:50)

Ala Cys Thr Gly Cys Tyr Asp Phe

Cys Cys Glu Arg Cys Cys Asn Pro	(SEQ ID NO:51)

Ala Cys Thr Gly Cys Tyr Asp Phe

Cys Cys Glu Lys Cys Cys Asn Pro	(SEQ ID NO:52)

Ala Cys Thr Gly Cys Tyr Asp Phe

Asn Cys Cys Glu Leu Cys Cys Asn	(SEQ ID NO:53)

Pro Ala Cys Thr Gly Cys Tyr Asp

Phe

Asn Cys Cys Glu Leu Cys Cys Asn	(SEQ ID NO:54)

Pro Ala Cys Trp Gly Cys Tyr Asp

Phe

Asn Cys Cys Glu Phe Cys Cys Asn	(SEQ ID NO:55)

Pro Ala Cys Thr Gly Cys Tyr Asp

Phe

Asn Cys Cys Glu Tyr Cys Cys Asn	(SEQ ID NO:56)

Pro Ala Cys Thr Gly Cys Tyr Asp

Phe

Asn Cys Cys Glu Trp Cys Cys Asn	(SEQ ID NO:57)

Pro Ala Cys Thr Gly Cys Tyr Asp

Phe

Asn Cys Cys Glu Arg Cys Cys Asn	(SEQ ID NO:58)

Pro Ala Cys Thr Gly Cys Tyr Asp

Phe

Asn Cys Cys Glu Lys Cys Cys Asn	(SEQ ID NO:59)

Pro Ala Cys Thr Gly Cys Tyr Asp

Phe

In some cases, the peptides of the invention are produced as a prepro protein that includes the amino terminal leader sequence: mkksilfiflsvlsfspfaqdakpvesskekitleskkcniakksnksgpesmn (SEQ ID NO:23). Where the peptide is produced by a bacterial cell, e.g., [0104] E. coli, the forgoing leader sequence will be cleaved and the mature peptide will be efficiently secreted from the bacterial cell. U.S. Pat. No. 5,395,490 describes vectors, expression systems and methods for the efficient production of ST peptides in bacterial cells and methods for achieving efficient secretion of mature ST peptides. The vectors, expression systems and methods described in U.S. Pat. No. 5,395,490 can be used to produce the ST peptides and variant ST peptides of the present invention
Variant Peptides [0105]

The invention includes variant peptides which can include one, two, three, four, five, six, seven, eight, nine, or ten (in some embodiments fewer than 5 or fewer than 3 or 2 or fewer) amino acid substitutions compared to SEQ ID NOs:25 to 59 and 66 to 110. The substitution(s) can be conservative or non-conservative. The naturally-occurring amino acids can be substituted by D-isomers of any amino acid, non-natural amino acids, and other groups. A conservative amino acid substitution results in the alteration of an amino acid for a similar acting amino acid, or amino acid of like charge, polarity, or hydrophobicity. At some positions, even conservative amino acid substitutions can reduce the activity of the peptide. Among the naturally occurring amino acid substitutions generally considered conservative are:



For Amino Acid	Code	Replace with any of

Alanine	Ala	Gly, Cys, Ser
Arginine	Arg	Lys, His
Asparagine	Asn	Asp, Glu, Gln,
Aspartic Acid	Asp	Asn, Glu, Gln
Cysteine	Cys	Met, Thr, Ser
Glutamine	Gln	Asn, Glu, Asp
Glutamic Acid	Glu	Asp, Asn, Gln
Glycine	Gly	Ala
Histidine	His	Lys, Arg
Isoleucine	Ile	Val, Leu, Met
Leucine	Leu	Val, Ile, Met
Lysine	Lys	Arg, His
Methionine	Met	Ile, Leu, Val
Phenylalanine	Phe	Tyr, His, Trp
Proline	Pro
Serine	Ser	Thr, Cys, Ala
Threonine	Thr	Ser, Met, Val
Tryptophan	Trp	Phe, Tyr
Tyrosine	Tyr	Phe, His
Valine	Val	Leu, Ile, Met

In some circumstances it can be desirable to treat patients with a variant peptide that binds to and activates intestinal GC—C receptor, but is less active than the non-variant form the peptide. This reduced activity can arise from reduced affinity for the receptor or a reduced ability to activate the receptor once bound or reduced stability of the peptide. [0107]
In some peptides pairs of Cys residues which normally form a disulfide bond one or both members of the pair can be replaced by homocysteine, 3-mercaptoproline (Kolodziej et al. 1996 Int J Pept Protein Res 48:274); β, β dimethylcysteine (Hunt et al. 1993 Int J Pept Protein Res 42:249) or diaminopropionic acid (Smith et al. 1978 J Med Chem 21:117) to form alternative internal cross-links at the positions of the normal disulfide bonds. [0108]
Production of Peptides [0109]
Useful peptides can be produced either in bacteria including, without limitation, [0110] E. coli, or in other existing systems for peptide or protein production (e.g., Bacillus subtilis, baculovirus expression systems using Drosophila Sf9 cells, yeast or filamentous fungal expression systems, mammalian cell expression systems), or they can be chemically synthesized.
If the peptide or variant peptide is to be produced in bacteria, e.g., [0111] E. coli, the nucleic acid molecule encoding the peptide will preferably also encode a leader sequence that permits the secretion of the mature peptide from the cell. Thus, the sequence encoding the peptide can include the pre sequence and the pro sequence of, for example, a naturally-occurring bacterial ST peptide. The secreted, mature peptide can be purified from the culture medium.
The sequence encoding a peptide of the invention is preferably inserted into a vector capable of delivering and maintaining the nucleic acid molecule in a bacterial cell. The DNA molecule may be inserted into an autonomously replicating vector (suitable vectors include, for example, pGEM3Z and pcDNA3, and derivatives thereof). The vector nucleic acid may be a bacterial or bacteriophage DNA such as bacteriophage lambda or M13 and derivatives thereof. Construction of a vector containing a nucleic acid described herein can be followed by transformation of a host cell such as a bacterium. Suitable bacterial hosts include but are not limited to, [0112] E. coli, B. subtilis, Pseudomonas, Salmonella. The genetic construct also includes, in addition to the encoding nucleic acid molecule, elements that allow expression, such as a promoter and regulatory sequences. The expression vectors may contain transcriptional control sequences that control transcriptional initiation, such as promoter, enhancer, operator, and repressor sequences. A variety of transcriptional control sequences are well known to those in the art. The expression vector can also include a translation regulatory sequence (e.g., an untranslated 5′ sequence, an untranslated 3′ sequence, or an internal ribosome entry site). The vector can be capable of autonomous replication or it can integrate into host DNA to ensure stability during peptide production.
The protein coding sequence that includes a peptide of the invention can also be fused to a nucleic acid encoding a polypeptide affinity tag, e.g., glutathione S-transferase (GST), maltose E binding protein, protein A, FLAG tag, hexa-histidine, myc tag or the influenza HA tag, in order to facilitate purification. The affinity tag or reporter fusion joins the reading frame of the peptide of interest to the reading frame of the gene encoding the affinity tag such that a translational fusion is generated. Expression of the fusion gene results in translation of a single polypeptide that includes both the peptide of interest and the affinity tag. In some instances where affinity tags are utilized, DNA sequence encoding a protease recognition site will be fused between the reading frames for the affinity tag and the peptide of interest. [0113]
Genetic constructs and methods suitable for production of immature and mature forms of the peptides and variants of the invention in protein expression systems other than bacteria, and well known to those skilled in the art, can also be used to produce peptides in a biological system. [0114]
Mature peptides and variants thereof can be synthesized by the solid-phase method using an automated peptide synthesizer. For example, the peptide can be synthesized on Cyc(4-CH[0115] ₂Bxl)-OCH₂-4-(oxymethyl)-phenylacetamidomethyl resin using a double coupling program. Protecting groups must be used appropriately to create the correct disulfide bond pattern. For example, the following protecting groups can be used: t-butyloxycarbonyl (alpha-amino groups); acetamidomethyl (thiol groups of Cys residues B and E); 4-methylbenyl (thiol groups of Cys residues C and F); benzyl (y-carboxyl of glutamic acid and the hydroxyl group of threonine, if present); and bromobenzyl (phenolic group of tyrosine, if present). Coupling is effected with symmetrical anhydride of t-butoxylcarbonylamino acids or hydroxybenzotriazole ester (for asparagine or glutamine residues), and the peptide is deprotected and cleaved from the solid support in hydrogen fluoride, dimethyl sulfide, anisole, and p-thiocresol using 8/1/1/0.5 ratio (v/v/v/w) at 0° C. for 60 min. After removal of hydrogen fluoride and dimethyl sulfide by reduced pressure and anisole and p-thiocresol by extraction with ethyl ether and ethyl acetate sequentially, crude peptides are extracted with a mixture of 0.5M sodium phosphate buffer, pH 8.0 and N,N-dimethylformamide using 1/1 ratio, v/v. The disulfide bond for Cys residues B and E is the formed using dimethyl sulfoxide (Tam et al. (1991) J. Am. Chem. Soc. 113:6657-62). The resulting peptide is the purified by reverse-phase chromatography. The disulfide bond between Cys residues C and F is formed by first dissolving the peptide in 50% acetic acid in water. Saturated iodine solution in glacial acetic acid is added (1 ml iodine solution per 100 ml solution). After incubation at room temperature for 2 days in an enclosed glass container, the solution is diluted five-fold with deionized water and extracted with ethyl ether four times for removal of unreacted iodine. After removal of the residual amount of ethyl ether by rotary evaporation the solution of crude product is lyophilized and purified by successive reverse-phase chromatography.
Intestinal GC—C Receptor Binding Assay [0116]
The ability of peptides and other agents to bind to the intestinal GC—C receptor can be tested as follows. Cells of the T84 human colon carcinoma cell line (American Type Culture Collection (Bethesda, Md.) are grown to confluence in 24-well culture plates with a 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium (DMEM), supplemented with 5% fetal calf serum. Cells used in the assay are typically between passages 54-60. Briefly, T84 cell monolayers in 24-well plates are washed twice with 1 ml of binding buffer (DMEM containing 0.05% bovine serum albumin and 25 mM HEPES, pH 7.2), then incubated for 30 min at 37° C. in the presence of mature radioactively labeled [0117] E. coli ST peptide and the test material at various concentrations. The cells are then washed four times with 1 ml of DMEM and solubilized with 0.5 ml/well 1N NaOH. The level of radioactivity in the solubilized material is then determined using standard methods.

EXAMPLE 1

Preparation of Variant ST Peptides and Wild-Type ST Peptide

1a: Preparation of Recombinant Variant ST Peptides and Wild-Type ST Peptide

A variant ST peptide, referred to as MD-915, was reproduced recombinantly and tested in an animal model. MD-915 has the sequence: Asn Ser Ser Asn Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr (SEQ ID NO:28). A peptide having the sequence of the wild-type ST peptide was also created (MM-416776). MD-915 and MM-416776 peptides were produced as preproproteins using vectors produced as follows. A sequence encoding a heat-stable enterotoxin pre-pro sequence was amplified from pGK51/pGSK51 (ATCC 67728) using oligonucleotide MO3514 (5′[0118] CACACCATATGAAGAAATCAATATTATTTATTTTTCTTTCTG 3′ (SEG ID NO:60)) and oligonucelotide M03515 (5′CACACCTCGAGTTAGGTCTCCATGCTTTCAGGACCACTTTTATTAC 3′ (SEQ ID NO: 61)). The amplification product fragment was digested with NdeI/XhoI and ligated to the T7 expression vector, pET26b(+) (Novagen) digested with NdeI/XhoI thereby creating plasmid MB3976. The region encoding the pre-pro protein was sequenced and found to encode the amino acid sequence: mkksilfiflsvlsfspfaqdakpagsskekitleskkcnivkksnksgpesm (SEQ ID NO: 24) which differs from the amino acid sequence of heat-stable enterotoxin a2 precursor (sta2; mkksilfiflsvlsfspfaqdakpagsskekitleskkcnivkknnesspesm (SEQ ID NO:25); GenBank® Accession No. Q47185, GI: 3913876) at three positions (indicated by underlining and bold text) near the C-terminus. To create expression vectors with the pre-pro sequence, complementary oligos encoding each ST peptide variant or wild-type ST peptide were annealed and cloned into the MB3976 expression vector. To create MB3984 (encoding MM-416776 peptide full length wild-type ST peptide as a prepro protein), containing the amino acid sequence, NSSNYCCELCCNPACTGCY (SEQ ID NO:26) fused downstream of the pre-pro sequence, MB 3976 was digested with BsaI/XhoI and ligated to annealed oligos MO3621 (5′GCATGAATAGTAGCAATTACTGCTGTGAATTGTGTTGTAATCCTGCTTGTACCGGGT GCTATTAATAAC 3′ (SEQ ID NO:62)) and MO3622 (5′TCGAGTTATTAATAGCACCCGGTACAAGCAGGATTACAACACAATTCACAGCAGTA ATTGCTACTATTC 3′ (SEQ ID NO:63)). To create MB3985 (encoding MD-915 as a prepro protein) containing the following amino acid sequence, NSSNYCCEYCCNPACTGCY (SEQ ID NO:28) fused downstream of the pre-pro sequence, MB 3976 was digested with BsaI/XhoI and ligated to annealed oligos M03529 (5′GCATGAATAGTAGCAATTACTGCTGTGAATATTGTTGTAATCCTGCTTGTACCGGGT GCTATTAATAAC 3′ (SEQ ID NO:64)) and MO3530 (5′TCGAGTTATTAATAGCACCCGGTACAAGCAGGATTACAACAATATTCACAGCAGTA ATTGCTACTATTC 3′ (SEQ ID NO:65)).
The MD-915 peptide and the MM-416776 peptide were produced as follows. The expression vectors were transformed into [0119] E. coli bacterial host BL21 λ DE3 (Invitrogen). A single colony was innoculated and grown shaking overnight at 30° C. in L broth+25 mg/l kanamycin. The overnight culture was added to 3.2 L of batch medium (Glucose 25 g/l, Caseamino Acids 5 g/l, Yeast Extract 5 g/l, KH₂PO₄13.3 g/l, (NH₄)₂HPO₄4 g/l, MgSO₄-7H ₂0 1.2 g/l, Citric Acid 1.7 g/l, EDTA 8.4 mg/l, CoCl₂-6H₂O 2.5 mg/l, MnCl₂-4H₂O 15 mg/l, CuCl₂-4H ₂0 1.5 mg/l, H₃BO₃3 mg/l, Na₂MoO₄-2H ₂0 2.5 mg/l, Zn Acetate-2H ₂0 13 mg/l, Ferric Citrate 100 mg/l, Kanamycin 25 mg/l, Antifoam DF ₂0₄1 ml/l) and fermented using the following process parameters: pH 6.7—control with base only (28% NH₄OH), 30° C., aeration: 5 liters per minute. After the initial consumption of batch glucose (based on monitoring dissolved oxygen (DO) levels), 1.5 L of feed medium (Glucose 700 g/l, Caseamino Acids 10 g/l, Yeast Extract 10 g/l, MgSO₄-7H ₂0 4 g/l, EDTA 13 mg/l, CoCl₂-6H₂O 4 mg/l, MnCl₂-4H₂O 23.5 mg/l, CuCl₂-4H ₂0 2.5 mg/l, H₃BO₃5 mg/l, Na₂MoO₄-2H ₂0 4 mg/l, Zn Acetate-2H ₂0 16 mg/l, Ferric Citrate 40 mg/l, Antifoam DF ₂0₄1 ml/l) was added at a feed rate controlled to maintain 20% DO. IPTG was added to 0.2 mM 2 hours post feed start. The total run time was approximately 40-45 hours (until feed exhaustion).
Cells were collected by centrifugation at 5,000 g for 10 minutes. The cell pellet was discarded and the supernatant was passed through a 50 Kd ultrafiltration unit. The 50 Kd filtrate (0.6 liters) was loaded onto a 110 ml Q-Sepharose fast Flow column (Amersham Pharmacia, equilibrated with 20 mM Tris-HCl pH 7.5) at a flow rate of 400 ml/hour. The column was washed with six volumes of 20 mM Tris-HCl pH 7.5 and proteins were eluted with 50 mM acetic acid collecting 50 ml fractions. Fractions containing ST peptide variant or wild-type ST peptide were pooled and the solvent was removed by rotary evaporation. The dried proteins were resuspended in 10 ml of 8% acetic acid, 0.1% trifluoroacetic acid (TFA) and loaded onto a Varian Polaris C18-A column (250×21.2 [0120] mm 10 μm, equilibrated in the same buffer) at a flow rate of 20 ml/min. The column was washed with 100 ml of 8% methanol, 0.1% TFA and developed with a gradient (300 ml) of 24 to 48% methanol, 0.1% TFA, collecting 5-ml fractions. Fractions containing peptide were pooled and the solvent was removed by rotary evaporation. The peptides were dissolved in 0.1%TFA and lyophilized.
The MD-915 peptide and MM-416776 peptide fractions were analyzed by standard LCMS and HPLC. LCMS analysis revealed that MD-915 is more homogeneous than MM-416776 (see FIG. 1[0121] a; note that MD-915 peptide exhibits fewer peaks (Panel B) than MM-416776 (Panel A)).

1b: Preparation of Synthetic Variant ST Peptides and Wild-Type ST Peptide

Peptides were chemically synthesized by a commercial peptide synthesis company. Varying yields of peptides were obtained depending on the efficiency of chemical synthesis. Thus, the four peptides, in decreasing order of yield were: Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr (SEQ ID NO:31; MD-1100), 10-20% yield; Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr (SEQ ID NO:29; MM416774); Asn Ser Ser Asn Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr (SEQ ID NO:28; MD-915); Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr (SEQ ID NO:26 MM-416776), <5% yield. Thus the specific amino acid changes introduced into the peptides can create improved manufacturing properties. [0122]
FIG. 1[0123] b shows the total ion chromatograph profile of synthetically manufactured MD-1100. FIG. 1c shows the total ion chromatograph profile of the control blank sample. There is one major peak present in the MD-1100 sample that is not also present in the control sample. Quantitative analysis suggests the MD-1100 is >98% pure.

EXAMPLE 2

Activation of the Intestinal GC—C Receptor by a Variant ST Peptide and ST Peptide

The ability of MD-915, MM-416776, and MD-1100 to activate the intestinal GC—C receptor was assessed in an assay employing the T84 human colon carcinoma cell line (American Type Culture Collection (Bethesda, Md.). For the assays cells were grown to confluency in 24-well culture plates with a 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium (DMEM), supplemented with 5% fetal calf serum and were used at between [0124] passages 54 and 60.
Briefly, monolayers of T84 cells in 24-well plates were washed twice with 1 ml/well DMEM, then incubated at 37° C. for 10 min with 0.45 ml DMEM containing 1 mM isobutylmethylxanthine (IBMX), a cyclic nucleotide phosphodiesterase inhibitor. Test peptides (50μl) were then added and incubated for 30 minutes at 37° C. The media was aspirated and the reaction was then terminated by the addition of ice cold 0.5 ml of 0.1N HCl. The samples were held on ice for 20 minutes and then evaporated to dryness using a heat gun or vacuum centrifugation. The dried samples were resuspended in 0.5 ml of phosphate buffer provided in the Cayman Chemical Cyclic GMP EIA kit (Cayman Chemical, Ann Arbor, Mich.). Cyclic GMP was measured by EIA according to procedures outlined in the Cayman Chemical Cyclic GMP EIA kit. [0125]
FIG. 2 shows the activity of chemically synthesized peptide variants in this GC—C receptor activity assay. In this assay, MM-416776 and two different MD-1100 peptides (MD-1100(a) and MD-1100(b), synthesized by two different methods) had activity comparable to MM-416776. MD-915 and MM-416776 peptide were chemically synthesized in a manner identical to that of MD-1100(b). [0126]

EXAMPLE 3

MD-915 and MM-416776 Increase Intestinal Transit in Mice

In order to determine whether the peptides increase the rate of gastrointestinal transit, the peptides and controls were tested using a murine gastrointestinal transit (GIT) assay (Moon et al. [0127] Infection and Immunity 25:127, 1979). In this assay, charcoal, which can be readily visualized in the gastrointestinal tract is administered to mice after the administration of a test compound. The distance traveled by the charcoal is measured and expressed as a percentage of the total length of the colon.
Mice were fasted with free access to water for 12 to 16 hours before the treatment with peptide or control buffer. The peptides were orally administered at 1 μg/kg-1 mg/kg of peptide in buffer (20 mM Tris pH 7.5) 7 minutes before being given an oral dose of 5% Activated Carbon (Aldrich 242276-250G). Control mice were administered buffer only before being given a dose of Activated Carbon. After 15 minutes, the mice were sacrificed and their intestines from the stomach to the cecum were dissected. The total length of the intestine as well as the distance traveled from the stomach to the charcoal front was measured for each animal and the results are expressed as the percent of the total length of the intestine traveled by the charcoal front. All results are reported as the average of 10 mice±standard deviation. A comparison of the distance traveled by the charcoal between the mice treated with peptide versus the mice treated with vehicle alone was performed using a Student's t test and a statistically significant difference was considered for P<0.05. P-values are calculated using a two-sided T-Test assuming unequal variances. [0128]
As can be seen in FIG. 3[0129] a, b, wild-type ST peptide (MM-416776, (Sigma-Aldrich, St Louis, Mo.; 0.1 mg/kg), synthetically manufactured MD-1100 and Zelnorm® (0.1 mg/kg), a drug approved for IBS that is an agonist for the serotonin receptor 5HT4, increase gastrointestinal transit rate in this model. FIG. 4a shows the result of a study demonstrating that intestinal transit rate increases with an increasing dosage of either recombinantly synthesized MM-416776 or MD-915. FIG. 4b shows the results of a study demonstrating both chemically synthesized MM-416776 or MD-1100 peptide increase intestinal transit rates more than either Tris buffer alone or an equivalent dose of Zelnorm®.
The identical experiment was performed to determine if MD-1100 is effective in a chronic dosing treatment regimen. Briefly, 8 week old CD1 female mice are dosed orally once a day for 5 days with either MD-1100 (0.06 mg/kg or 0.25 mg/kg in 20 mM Tris pH 7.5) or vehicle alone (20 mM Tris pH 7.5). On the 5[0130] ^thday, a GIT assay is performed identical to that above except 200 μl of a 10% charcoal solution is administered. FIG. 4c shows the results of a study demonstrating both chemically synthesized MD-1100 or Zelnorm® are effective in a mouse gastrointestinal motility assay upon chronic dosing (daily for 5 days). The results are shown side by side with acute dosing (1 day).

EXAMPLE 4

MD-915 Peptide and MM-416776 Peptide Increase Intestinal Secretion in Suckling Mice (SuMi Assay)

MM-416776 peptide and MD-915 were tested for their ability to increase intestinal secretion using a suckling mouse model of intestinal secretion. In this model a test compound is administered to suckling mice that are between 7 and 9 days old. After the mice are sacrificed, the gastrointestinal tract from the stomach to the cecum is dissected (“guts”). The remains (“carcass”) as well as the guts are weighed and the ratio of guts to carcass weight is calculated. If the ratio is above 0.09, one can conclude that the test compound increases intestinal secretion. FIG. 5[0131] a shows a dose response curve for wild-type ST peptide (MM-416776) in this model. FIG. 5b shows dose response curve for the MD-1100 peptide in this model. These data show that wild-type ST peptide (purchased from TDT, Inc. West Chester, Pa.) and the MD-1100 peptide increase intestinal secretion. The effect of Zelnorm® was also studied. As can be seen from FIG. 5, Zelnorm® at 0.2 mg/kg does not increase intestinal secretion in this model. FIG. 6a shows a dose response curve for the recombinant MM-416776 peptide described above and the recombinant MD-915 peptide described above. As can be seen from FIG. 6a, both peptides increase intestinal secretion in this model. Similarly FIG. 6b shows a dose response curve for chemically synthesized MD-915, MD-1100 and MM-416776 as well as wild-type ST peptide (purchased from Sigma-Aldrich, St Louis, Mo.).
Colonic Hyperalgesia Animal Models [0132]
Hypersensitivity to colorectal distension is common in patients with IBS and may be responsible for the major symptom of pain. Both inflammatory and non-inflammatory animal models of visceral hyperalgesia to distension have been developed to investigate the effect of compounds on visceral pain in IBS. [0133]

I. Trinitrobenzenesulphonic Acid (TNBS)-Induced Rectal Allodynia Model

Male Wistar rats (220-250 g) were premedicated with 0.5 mg/kg of acepromazine injected intraperitoneally (IP) and anesthetized by intramuscular administration of 100 mg/kg of ketamine. Pairs of nichrome wire electrodes (60 cm in length and 80 μm in diameter) were implanted in the striated muscle of the abdomen, 2 cm laterally from the white line. The free ends of electrodes were exteriorized on the back of the neck and protected by a plastic tube attached to the skin. Electromyographic (EMG) recordings were started 5 days after surgery. Electrical activity of abdominal striated muscle was recorded with an electroencephalograph machine (Mini VIII, Alvar, Paris, France) using a short time constant (0.03 sec.) to remove low-frequency signals (<3 Hz). [0134]
Ten days post surgical implantation, trinitrobenzenesulphonic acid (TNBS) was administered to induce rectal inflammation. TNBS (80 mg kg[0135] ⁻¹in 0.3 ml 50% ethanol) was administered intrarectally through a silicone rubber catheter introduced at 3 cm from the anus under light diethyl-ether anesthesia, as described (Morteau et al. 1994 Dig Dis Sci 39:1239). Following TNBS administration, rats were placed in plastic tunnels where they were severely limited in mobility for several days before colorectal distension (CRD). Experimental compound was administered one hour before CRD which was performed by insertion into the rectum, at 1 cm of the anus, a 4 cm long balloon made from a latex condom (Gue et al, 1997 Neurogastroenterol. Motil. 9:271). The balloon was fixed on a rigid catheter taken from an embolectomy probe (Fogarty). The catheter attached balloon was fixed at the base of the tail. The balloon, connected to a barostat, was inflated progressively by step of 15 mmHg, from 0 to 60 mmHg, each step of inflation lasting 5 min. Evaluation of rectal sensitivity, as measured by EMG, was performed before (1-2 days) and 3 days following rectal instillation of TNBS.
The number of spike bursts that corresponds to abdominal contractions was determined per 5 min periods. Statistical analysis of the number of abdominal contractions and evaluation of the dose-effects relationships was performed by a one way analysis of variance (ANOVA) followed by a post-hoc (Student or Dunnett tests) and regression analysis for ED50 if appropriate. [0136]
FIG. 7 shows the results of experiment in which MD-1100 activity was analyzed in the TNBS colorectal model. Significant decreases in abdominal response are observed at 0.3 μg/kg and 3 μg/kg MD-1100. These results demonstrate that MD-1100 reduces pain associated with colorectal distension in this animal model. [0137]

II. Stress-Induced Hyperalgesia Model

Male Wistar Rats (200-250 g) are surgically implanted with nichrome wire electrodes as in the TNBS model. Ten days post surgical implantation, partial restraint stress (PRS), is performed as described by Williams et al. for two hours (Williams et al. 1988 Gastroenterology 64:611). Briefly, under light anesthesia with ethyl-ether, the foreshoulders, upper forelimbs and thoracic trunk are wrapped in a confining harness of paper tape to restrict, but not prevent body movements. Control sham-stress animals are anaesthetized but not wrapped. Thirty minutes before the end of the PRS session, the animals are administered test-compound or vehicle. Thirty minutes to one hour after PRS completion, the CRD distension procedure is performed as described above for the TNBS model with barostat at pressures of 15, 30, 45 and 60 mm Hg. Statistical analysis on the number of bursts is determined and analyzed as in the TNBS model above. [0138]
Phenylbenzoguinone-Induced Writhing Model [0139]
The PBQ-induced writhing model can be used to assess pain control activity of the peptides and GC—C receptor agonists of the invention. This model is described by Siegmund et al. (1957 Proc. Soc. Exp. Bio. Med. 95:729-731). Briefly, one hour after oral dosing with a test compound, e.g., a peptide, morphine or vehicle, 0.02% phenylbenzoquinone (PBQ) solution (12.5 mL/kg) is injected by intraperitoneal route into the mouse. The number of stretches and writhings are recorded from the 5[0140] ^thto the 10^thminute after PBQ injection, and can also be counted between the 35^thand 40^thminute and between the 60^thand 65^thminute to provide a kinetic assessment. The results are expressed as the number of stretches and writhings (mean±SEM) and the percentage of variation of the nociceptive threshold calculated from the mean value of the vehicle-treated group. The statistical significance of any differences between the treated groups and the control group is determined by a Dunnett's test using the residual variance after a one-way analysis of variance (P<0.05) using SigmaStat Software.
FIGS. 8[0141] a and 8 b show the effect of different doses of MD-915 and MD-1100 in the PBQ writhing assay. Indomethacin, an NSAID (nonsteroidal anti-inflammatory drug) with known pain control activity, was used as the positive control in the assay. Significant reductions in writhings were observed for MD-915 (1 mg/kg dose) and MD-1100 (2.5 mg/kg dose) compared to the vehicle control. Loss of efficacy at the highest dose tested has also been observed for multiple other compounds (such as 5HT-3 antagonists) tested in similar assays. The results of this study suggest that both MD-915 and MD-1100 have antinociceptive effects in this visceral pain model comparable to the intermediate doses of indomethacin.

EXAMPLE 5

MD-1100 Kd Determination

To determine the affinity of MD-1100 for GC—C receptors found in rat intestinal mucosa, a competition binding assay was performed using rate intestinal epithelial cells. Epithelial cells from the small intestine of rats were obtained as described by Kessler et al. ([0142] J. Biol. Chem. 245: 5281-5288 (1970)). Briefly, animals were sacrificed and their abdominal cavities exposed. The small intestine was rinsed with 300 ml ice cold saline or PBS. 10 cm of the small intestine measured at 10 cm from the pylorus was removed and cut into 1 inch segments. Intestinal mucosa was extruded from the intestine by gentle pressure between a piece of parafilm and a P-1000 pipette tip. Intestinal epithelial cells were placed in 2 ml PBS and pipetted up and down with a 5 ml pipette to make a suspension of cells. Protein concentration in the suspension was measured using the Bradford method (Anal. Biochem. 72: 248-254 (1976)).
A competition binding assay was performed based on the method of Giannella et al. ([0143] Am. J. Physiol. 245: G492-G498) between [¹²⁵I] labeled MM-416776 and MD-1100. The assay mixture contained: 0.5 ml of DME with 20 mM HEPES-KOH pH 7.0, 0.9 mg of the cell suspension listed above, 21.4 fmol[¹²⁵I]-MM-416776 (42.8 pM), and different concentrations of competitor MD-1100 (0.01 to 1000 nM). The mixture was incubated at room temperature for 1 hour, and the reaction stopped by applying the mixture to GF/B glass-fiber filters (Whatman). The filters were washed with 5 ml ice-cold PBS and radioactivity was measured. FIG. 9 shows that the Kd for MD-1100 in this assay is 4.5 nm. %B/Bo is the percentage of the ratio of radioactivity trapped in each sample (B) compared to the radioactivity retained in a control sample with no cold competitor (Bo). Giannella et al. (Am. J Physiol.245: G492-G498) observed that the Kd for wild-type ST peptide in this same assay was˜13 nm.

EXAMPLE 6

Pharmacokinetic properties of MD-1100

To study the pharmacokinetics of MD-1100, absorbability studies in mice were performed by administering MD-1100 intravaneously via tail vein injection or orally by gavage to 8-week-old CD1 mice. Serum was collected from the animals at various time points and tested for the presence of MD-1100 using a competitive enzyme-linked immunoabsorbent assay (Oxoid, ST EIA kit, Cat#TD0700). The assay utilized monoclonal antibodies against ST peptide (antibodies are provided in the Oxoid kit) and synthetically manufactured MD-1100. FIG. 10[0144] a show absorption data for intravenously and orally administered MD-1100 as detected by the ELISA assay. MD-1100 appears to be minimally systemically absorbed and is <2.2% bioavailable.
A similar bioavailability study was performed in which LCMS rather than ELISA was used to detect MD-1100. Initially, serum samples were extracted from the whole blood of exposed and control mice, then injected directly (10 mL) onto an in-line solid phase extraction (SPE) column ([0145] Waters Oasis HLB 25 mm column, 2.0×15 mm direct connect) without further processing. The sample on the SPE column was washed with a 5% methanol, 95% dH₂O solution (2.1 mL/min, 1.0 minute), then loaded onto an analytical column using a valve switch that places the SPE column in an inverted flow path onto the analytical column (Waters Xterra MS C8 5 mm IS column, 2.1×20 mm). The sample was eluted from the analytical column with a reverse phase gradient (Mobile Phase A: 10 mM ammonium hydroxide in dH₂O, Mobile Phase B: 10 mM ammonium hydroxide in 80% acetonitrile and 20% methanol; 20% B for the first 3 minutes then ramping to 95% B over 4 min. and holding for 2 min., all at a flow rate of 0.4 mL/min.). At 9.1 minutes, the gradient returns to the initial conditions of 20%B for 1 min. MD-1100 eluted from the analytical column at 1.45 minutes, and was detected by triple-quadrapole mass spectrometry (MRM, 764 (+2 charge state)>182 (+1 charge state) Da; cone voltage=30V; collision=20 eV; parent resolution=2 Da at base peak; daughter resolution=2 Da at base peak). Instrument response was converted into concentration units by comparison with a standard curve using known amounts of chemically synthesized MD-1100 prepared and injected in mouse serum using the same procedure.
FIG. 10[0146] b shows absorption data for IV and orally administered MD-1100 as detected by LCMS. In this assay, MD-1100 appears similarly minimally systemically absorbed and is <0.11% bioavailable.
Administration of Peptides and GC—C Receptor Agonists [0147]
For treatment of gastrointestinal disorders, the peptides and agonists of the invention are preferably administered orally, e.g., as a tablet, gel, paste, slurry, liquid, powder or in some other form. Orally administered compositions can include binders, flavoring agents, and humectants. The peptides and agonists can be co-administered with other agents used to treat gastrointestinal disorders including but not limited to acid suppressing agents such as Histamine-2 receptor agonists (H2As) and proton pump inhibitors (PPIs). The peptides and agonists can also be administered by rectal suppository. For the treatment of disorders outside the gastrointestinal tract such as congestive heart failure and benign prostatic hypertrophy, peptides and agonists are preferably administered parenterally or orally. The peptides described herein can be used alone or in combination with other agents. For example, the peptides can be administered together with an analgesic peptide or compound. The analgesic peptide or compound can be covalently attached to a peptide described herein or it can be a separate agent that is administered together with or sequentially with a peptide described herein in a combination therapy. [0148]
Combination therapy can be achieved by administering two or more agents, e.g., a peptide described herein and an analgesic peptide or compound, each of which is formulated and administered separately, or by administering two or more agents in a single formulation. Other combinations are also encompassed by combination therapy. For example, two agents can be formulated together and administered in conjunction with a separate formulation containing a third agent. While the two or more agents in the combination therapy can be administered simultaneously, they need not be. For example, administration of a first agent (or combination of agents) can precede administration of a second agent (or combination of agents) by minutes, hours, days, or weeks. Thus, the two or more agents can be administered within minutes of each other or within 1, 2, 3, 6, 9, 12, 15, 18, or 24 hours of each other or within 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14 days of each other or within 2, 3, 4, 5, 6, 7, 8, 9, or 10 weeks of each other. In some cases even longer intervals are possible. While in many cases it is desirable that the two or more agents used in a combination therapy be present in within the patient's body at the same time, this need not be so. [0149]
Combination therapy can also include two or more administrations of one or more of the agents used in the combination. For example, if agent X and agent Y are used in a combination, one could administer them sequentially in any combination one or more times, e.g., in the order X—Y—X, X—X—Y, Y—X—Y, Y—Y—X, X—X—Y—Y, etc. [0150]
The agents, alone or in combination, can be combined with any pharmaceutically acceptable carrier or medium. Thus, they can be combined with materials that do not produce an adverse, allergic or otherwise unwanted reaction when administered to a patient. The carriers or mediums used can include solvents, dispersants, coatings, absorption promoting agents, controlled release agents, etc. [0151]
The agents either in their free form or as a salt can be combined with a polymer such as polylactic-glycoloic acid (PLGA), poly-(I)-lactic-glycolic-tartaric acid (P(I)LGT) (WO 01/12233), polyglycolic acid (U.S. Pat. No. 3,773,919), polylactic acid (U.S. Pat. No. 4,767,628); poly(ε-caprolactone) to create a sustained release formulation. Such formulations can be used to implants that release a peptide or another agent over a period of a few days, a few weeks or several months depending on the polymer, the particle size of the polymer, and the size of the implant (see, e.g., U.S. Pat. No. 6,620,422). Other sustained release formulations are described in [0152] EP 0 467 389 A2, WO 93/241150, U.S. Pat. No. 5,612,052; WO 97/40085, WO 94/155587, U.S. Pat. No. 5,672,659, U.S. Pat. No. 5,893,985, U.S. Pat. No. 5,134,122, U.S. Pat. No. 5,192,741, U.S. Pat. No. 5,192,741, and U.S. Pat. No. 5,445,832. In such sustained release formulations microparticles of peptide are combined with microparticles of polymer. One or more sustained release implants can be placed in the large intestine, the small intestine or both.
The agents can be administered, e.g., by intravenous injection, intramuscular injection, subcutaneous injection, or by other routes. The agents can be administered orally, e.g., as a tablet, gel, paste, slurry, liquid, powder or in some other form. Orally administered compositions can include binders, flavoring agents, and humectants. The agents can be included in dentifrices or oral washes. Thus, oral formulations can include abrasives and foaming agents. The agents can also be administered transdermally or in the form a suppository. [0153]
The agents can be a free acid or base, or a pharmacologically acceptable salt thereof. Solids can be dissolved or dispersed immediately prior to administration or earlier. In some circumstances the preparations include a preservative to prevent the growth of microorganisms. The pharmaceutical forms suitable for injection can include sterile aqueous or organic solutions or dispersions which include, e.g., water, an alcohol, an organic solvent, an oil or other solvent or dispersant (e.g., glycerol, propylene glycol, polyethylene glycol, and vegetable oils). Pharmaceutical agents can be sterilized by filter sterilization or by other suitable means. [0154]
Suitable pharmaceutical compositions in accordance with the invention will generally include an amount of the active compound(s) with an acceptable pharmaceutical diluent or excipient, such as a sterile aqueous solution, to give a range of final concentrations, depending on the intended use. The techniques of preparation are generally well known in the art, as exemplified by Remington's Pharmaceutical Sciences (18th Edition, Mack Publishing Company, 1995). [0155]
The agents described herein and combination therapy agents can be packaged as a kit that includes single or multiple doses of two or more agents, each packaged or formulated individually, or single or multiple doses of two or more agents packaged or formulated in combination. Thus, one or more agents can be present in first container, and the kit can optionally include one or more agents in a second container. The container or containers are placed within a package, and the package can optionally include administration or dosage instructions. A kit can include additional components such as syringes or other means for administering the agents as well as diluents or other means for formulation. [0156]
Analgesic Agents [0157]
The peptides described herein can be used in combination therapy with an analgesic agent, e.g., an analgesic compound or an analgesic peptide. The analgesic agent can optionally be covalently attached to a peptide described herein. Among the useful analgesic agents are: Ca channel blockers, 5HT receptor antagonists (for example 5HT3, 5HT4 and 5HT1 receptor antagonists), opioid receptor agonists (loperamide, fedotozine, and fentanyl), NK1 receptor antagonists, CCK receptor agonists (e.g., loxiglumide), NK1 receptor antagonists, NK3 receptor antagonists, norepinephrine-serotonin reuptake inhibitors (NSRI), vanilloid and cannabanoid receptor agonists, and sialorphin. Analgesics agents in the various classes are described in the literature. [0158]
Among the useful analgesic peptides are sialorphin-related peptides, including those comprising the amino acid sequence QHNPR (SEQ ID NO:111), including: VQHNPR (SEQ ID NO:112); VRQHNPR (SEQ ID NO:113); VRGQHNPR (SEQ ID NO:114); VRGPQHNPR (SEQ ID NO:115); VRGPRQHNPR (SEQ ID NO:116); VRGPRRQHNPR (SEQ ID NO:117); and RQHNPR (SEQ ID NO:118). Sialorphin-related peptides bind to neprilysin and inhibit neprilysin-mediated breakdown of substance P and Met-enkephalin. Thus, compounds or peptides that are inhibitors of neprilysin are useful analgesic agents which can be administered with the peptides of the invention in a co-therapy or linked to the peptides of the invention, e.g., by a covalent bond. Sialophin and related peptides are described in U.S. Pat. No. 6,589,750; U.S. 20030078200 A1; and WO 02/051435 A2. [0159]
Opioid receptor antagonists and agonists can be administered with the peptides of the invention in co-therapy or linked to the peptide of the invention, e.g., by a covalent bond. For example, opioid receptor antagonists such as naloxone, naltrexone, methyl nalozone, nalmefene, cypridime, beta funaltrexamine, naloxonazine, naltrindole, and nor-binaltorphimine are thought to be useful in the treatment of IBS. It can be useful to formulate opioid antagonists of this type is a delayed and sustained release formulation such that initial release of the antagonist is in the mid to distal small intestine and/or ascending colon. Such antagonists are described in WO 01/32180 A2. Enkephalin pentapeptide (HOE825; Tyr-D-Lys-Gly-Phe-L-homoserine) is an agonist of the mu and delta opioid receptors and is thought to be useful for increasing intestinal motility ([0160] Eur. J Pharm. 219:445, 1992), and this peptide can be used in conjunction with the peptides of the invention. Also useful is trimebutine which is thought to bind to mu/delta/kappa opioid receptors and activate release of motilin and modulate the release of gastrin, vasoactive intestinal peptide, gastrin and glucagons. Kappa opioid receptor agonists such as fedotozine, ketocyclazocine, and compounds described in WO 03/097051 A2 can be used with or linked to the peptides of the invention. In addition, mu opioid receptor agonists such as morphine, diphenyloxylate, frakefamide (H-Tyr-D-Ala-Phe(F)-Phe-NH₂; WO 01/019849 A1) and loperamide can be used.
Tyr-Arg (kyotorphin) is a dipeptide that acts by stimulating the release of met-enkephalins to elicit an analgesic effect ([0161] J. Biol. Chem 262:8165, 1987). Kyotorphin can be used with or linked to the peptides of the invention.
CCK receptor agonists such as caerulein from amphibians and other species are useful analgesic agents that can be used with or linked to the peptides of the invention. [0162]
Conotoxin peptides represent a large class of analgesic peptides that act at voltage gated Ca channels, NMDA receptors or nicotinic receptors. These peptides can be used with or linked to the peptides of the invention. [0163]
Peptide analogs of thymulin (FR Application 2830451) can have analgesic activity and can be used with or linked to the peptides of the invention. [0164]
CCK (CCKa or CCKb) receptor antagonists, including loxiglumide and dexloxiglumide (the R-isomer of loxiglumide) (WO 88/05774) can have analgesic activity and can be used with or linked to the peptides of the invention. [0165]
Other useful analgesic agents include 5-HT4 agonists such as tegaserod/zelnorm and lirexapride. Such agonists are described in: EP1321142 A1, WO 03/053432A1, EP 505322 A1, EP 505322 B1, U.S. Pat. No. 5,510,353, EP 507672 A1, EP 507672 B1, and U.S. P 5,273,983. [0166]
Calcium channel blockers such as ziconotide and related compounds described in, for example, EP625162B1, U.S. Pat. No. 5,364,842, U.S. Pat. No. 5,587,454, U.S. Pat 5,824,645, U.S. Pat. No. 5,859,186, U.S. Pat. No. 5,994,305, U.S. Pat. No. 6,087,091, U.S. Pat. No. 6,136,786, WO 93/13128 A1, EP 1336409 A1, EP 835126 A1, EP 835126 B1, U.S. Pat. No. 5,795,864, U.S. Pat. No. 5,891,849, U.S. Pat. No. 6,054,429, WO 97/01351 A1, can be used with or linked to the peptides of the invention. [0167]
Various antagonists of the NK-1, NK-2, and NK-3 receptors (for a review see Giardina et al. 2003 [0168] Drugs 6:758) can be can be used with or linked to the peptides of the invention.
NK1 receptor antagonists such as: aprepitant (Merck & Co Inc), vofopitant, ezlopitant (Pfizer, Inc.), R-673 (Hoffmann-La Roche Ltd), SR-14033 and related compounds described in, for example, EP 873753 A1, US 20010006972 A1, US 20030109417 A1, WO 01/52844 A1, can be used with or linked to the peptides of the invention. [0169]
NK-2 receptor antagonists such as nepadutant (Menarini Ricerche SpA), saredutant (Sanofi-Synthelabo), SR-144190 (Sanofi-Synthelabo) and UK-290795 (Pfizer Inc) can be used with or linked to the peptides of the invention. [0170]
NK3 receptor antagonists such as osanetant (Sanofi-Synthelabo), talnetant and related compounds described in, for example, WO 02/094187 A2, EP 876347 A1, WO 97/21680 A1, U.S. Pat. No. 6,277,862, WO 98/11090, WO 95/28418, WO 97/19927, and Boden et al. ([0171] J Med Chem. 39:1664-75, 1996) can be used with or linked to the peptides of the invention.
Norepinephrine-serotonin reuptake inhibitors such as milnacipran and related compounds described in WO 03/077897 A1 can be used with or linked to the peptides of the invention. [0172]
Vanilloid receptor antagonists such as arvanil and related compounds described in WO 01/64212 A1 can be used with or linked to the peptides of the invention. [0173]
Where the analgesic is a peptide and is covalently linked to a peptide described herein the resulting peptide may also include at least one trypsin or chymotrypsin cleavage site. When present within the peptide, the analgesic peptide may be preceded by (if it is at the carboxy terminus) or followed by (if it is at the amino terminus) a chymotrypsin or trypsin cleavage site that allows release of the analgesic peptide. [0174]
In addition to sialorphin-related peptides, analgesic peptides include: AspPhe, endomorphin-1, endomorphin-2, nocistatin, dalargin, lupron, zicnotide, and substance P. [0175]
Methods of Treatment [0176]
The peptides of the invention can be used for the treatment or prevention of cancer, pre-cancerous growths, or metastatic growths. For example, they can be used for the prevention or treatment of: colorectal/local metastasized colorectal cancer, gastrointestinal tract cancer, lung cancer, cancer or pre-cancerous growths or metastatic growths of epithelial cells, polyps, breast, colorectal, lung, ovarian, pancreatic, prostatic, renal, stomach, bladder, liver, esophageal and testicular carcinoma, carcinoma (e.g., basal cell, basosquamous, Brown-Pearce, ductal carcinoma, Ehrlich tumor, Krebs, Merkel cell, small or non-small cell lung, oat cell, papillary, bronchiolar, squamous cell, transitional cell, Walker), leukemia (e.g., B-cell, T-cell, HTLV, acute or chronic lymphocytic, mast cell, myeloid), histiocytonia, histiocytosis, Hodgkin's disease, non-Hodgkin's lymphoma, plasmacytoma, reticuloendotheliosis, adenoma, adeno-carcinoma, adenofibroma, adenolymphoma, ameloblastoma, angiokeratoma, angiolymphoid hyperplasia with eosinophilia, sclerosing angioma, angiomatosis, apudoma, branchionia, malignant carcinoid syndrome, carcinoid heart disease, carcinosarcoma, cementoma, cholangioma, cholesteatoma, chondrosarcoma, chondroblastoma, chondrosarcoma, chordoma, choristoma, craniopharyngioma, chrondroma, cylindroma, cystadenocarcinoma, cystadenoma, cystosarconia phyllodes, dysgenninoma, ependymoma, Ewing sarcoma, fibroma, fibrosarcoma, giant cell tumor, ganglioneuroma, glioblastoma, glomangioma, granulosa cell tumor, gynandroblastoma, hamartoma, hemangioendothelioma, hemangioma, hemangio-pericytoma, hemangiosarcoma, hepatoma, islet cell tumor, Kaposi sarcoma, leiomyoma, leiomyosarcoma, leukosarcoma, Leydig cell tumor, lipoma, liposarcoma, lymphaugioma, lymphangiomyoma, lymphangiosarcoma, medulloblastoma, meningioma, mesenchymoma, mesonephroma, mesothelioma, myoblastoma, myoma, myosarcoma, myxoma, myxosarcoma, neurilemmoma, neuroma, neuroblastoma, neuroepithelioma, neurofibroma, neurofibromatosis, odontoma, osteoma, osteosarcoma, papilloma, paraganglioma, paraganglionia. nonchroinaffin, pinealoma, rhabdomyoma, rhabdomyosarcoma, Sertoli cell tumor, teratoma, theca cell tumor, and other diseases in which cells have become dysplastic, immortalized, or transformed. [0177]
The peptides of the invention can be used for the treatment or prevention of: Familial Adenomatous Polyposis (FAP) (autosomal dominant syndrome) that precedes colon cancer, hereditary nonpolyposis colorectal cancer (HNPCC), and inherited autosomal dominant syndrome. [0178]
For treatment or prevention of cancer, pre-cancerous growths and metastatic growths, the peptides can be used in combination therapy with radiation or chemotherapeutic agents, an inhibitor of a cGMP-dependent phosphodiesterase or a selective cyclooxygenase-2 inhibitor (a number of selective cyclooxygenase-2 inhibitors are described in WO02062369, hereby incorporated by reference). [0179]
The peptides can be for treatment or prevention of inflammation. Thus, they can be used alone or in combination with inhibitor of cGMP-dependent phosphodiesterase or a selective cyclooxygenase-2 inhibitor for treatment of: organ inflammation, IBD (e.g, Crohn's disease, ulcerative colitis), asthma, nephritis, hepatitis, pancreatitis, bronchitis, cystic fibrosis, ischemic bowel diseases, intestinal inflammations/allergies, coeliac disease, proctitis, eosnophilic gastroenteritis, mastocytosis, and other inflammatory disorders. [0180]
The peptides can also be used to treat or prevent insulin-related disorders, for example: II diabetes mellitus, hyperglycemia, obesity, disorders associated with disturbances in glucose or electrolyte transport and insulin secretion in cells, or endocrine disorders. They can be also used in insulin resistance treatment and post-surgical and non-post surgery decrease in insulin responsiveness. [0181]
The peptides can be used to prevent or treat respiratory disorders, including, inhalation, ventilation and mucus secretion disorders, pulmonary hypertension, chronic obstruction of vessels and airways, and irreversible obstructions of vessels and bronchi. [0182]
The peptides can be used in combination therapy with a phosphodiesterase inhibitor (examples of such inhibitors can be found in U.S. Pat. No. 6,333,354, hereby incorporated by reference). [0183]
The peptides can also be used to prevent or treat: retinopathy, nephropathy, diabetic angiopathy, and edema formation [0184]
The peptides can also be used to prevent or treat neurological disorders, for example, headache, anxiety, movement disorders, aggression, psychosis, seizures, panic attacks, hysteria, sleep disorders, depression, schizoaffective disorders, sleep apnea, attention deficit syndromes, memory loss, and narcolepsy. They may also be used as a sedative. [0185]
The peptides and detectabley labeled peptides can be used as markers to identify, detect, stage, or diagnosis diseases and conditions of the small intestine, including: Crohn's disease, colitis, inflammatory bowel disease, tumors, benign tumors, such as benign stromal tumors, adenoma, angioma, adenomatous (pedunculated and sessile) polyps, malignant, carcinoid tumors, endocrine cell tumors, lymphoma, adenocarcinoma, foregut, midgut, and hindgut carcinoma, gastroinstestinal stromal tumor (GIST), such as leiomyoma, cellular leiomyoma, leiomyoblastoma, and leiomyosarcoma, gastrointestinal autonomic nerve tumor, malabsorption syndromes, celiac diseases, diverticulosis, Meckel's diverticulum, colonic diverticula, megacolon, Hirschsprung's disease, irritable bowel syndrome, mesenteric ischemia, ischemic colitis, colorectal cancer, colonic polyposis, polyp syndrome, intestinal adenocarcinoma, Liddle syndrome, Brody myopathy, infantile convulsions, and choreoathetosis [0186]
The peptides can be conjugated to another molecule (e.g, a diagnostic or therapeutic molecule) to target cells bearing the GCC receptor, e.g., cystic fibrosis lesions and specific cells lining the intestinal tract. Thus, they can be used to target radioactive moieties or therapeutic moieties to the intestine to aid in imaging and diagnosing or treating colorectal/metastasized or local colorectal cancer and to deliver normal copies of the p53 tumor suppressor gene to the intestinal tract. [0187]
The peptides can be used alone or in combination therapy to treat erectile dysfunction. [0188]
The peptides can be used alone or in combination therapy to treat inner ear disorders, e.g., to treat Meniere's disease, including symptoms of the disease such as vertigo, hearing loss, tinnitus, sensation of fullness in the ear, and to maintain fluid homeostasis in the inner ear. [0189]
The peptides can be used alone or in combination therapy to treat disorders associated with fluid and sodium retention, e.g., diseases of the electrolyte-water/electrolyte transport system within the kidney, gut and urogenital system, congestive heart failure, hypertension, [0190]
1 124 1 19 PRT Escherichia coli 1 Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 2 18 PRT Escherichia coli 2 Asn Thr Phe Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Ala Gly 1 5 10 15 Cys Tyr 3 18 PRT Escherichia coli 3 Asn Thr Phe Tyr Cys Cys Glu Leu Cys Cys Tyr Pro Ala Cys Ala Gly 1 5 10 15 Cys Asn 4 18 PRT Citrobacter freundii 4 Asn Thr Phe Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Ala Gly 1 5 10 15 Cys Tyr 5 30 PRT Yersinia enterocolitica 5 Gln Ala Cys Asp Pro Pro Ser Pro Pro Ala Glu Val Ser Ser Asp Trp 1 5 10 15 Asp Cys Cys Asp Val Cys Cys Asn Pro Ala Cys Ala Gly Cys 20 25 30 6 30 PRT Yersinia enterocolitica 6 Lys Ala Cys Asp Thr Gln Thr Pro Ser Pro Ser Glu Glu Asn Asp Asp 1 5 10 15 Trp Cys Cys Glu Val Cys Cys Asn Pro Ala Cys Ala Gly Cys 20 25 30 7 53 PRT Yersinia enterocolitica 7 Gln Glu Thr Ala Ser Gly Gln Val Gly Asp Val Ser Ser Ser Thr Ile 1 5 10 15 Ala Thr Glu Val Ser Glu Ala Glu Cys Gly Thr Gln Ser Ala Thr Thr 20 25 30 Gln Gly Glu Asn Asp Trp Asp Trp Cys Cys Glu Leu Cys Cys Asn Pro 35 40 45 Ala Cys Phe Gly Cys 50 8 16 PRT Yersinia kristensenii 8 Ser Asp Trp Cys Cys Glu Val Cys Cys Asn Pro Ala Cys Ala Gly Cys 1 5 10 15 9 17 PRT Vibrio cholerae 9 Ile Asp Cys Cys Glu Ile Cys Cys Asn Pro Ala Cys Phe Gly Cys Leu 1 5 10 15 Asn 10 17 PRT Vibrio mimicus 10 Ile Asp Cys Cys Glu Ile Cys Cys Asn Pro Ala Cys Phe Gly Cys Leu 1 5 10 15 Asn 11 18 PRT Escherichia coli 11 Asn Thr Phe Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Ala Pro 1 5 10 15 Cys Tyr 12 13 PRT Vibrio cholerae 12 Ile Asp Cys Cys Glu Ile Cys Cys Asn Pro Ala Cys Phe 1 5 10 13 14 PRT Vibrio cholerae 13 Ile Asp Cys Cys Glu Ile Cys Cys Asn Pro Ala Cys Phe Gly 1 5 10 14 17 PRT Vibrio mimicus 14 Ile Asp Cys Cys Glu Ile Cys Cys Asn Pro Ala Cys Phe Gly Cys Leu 1 5 10 15 Asn 15 17 PRT Vibrio mimicus 15 Ile Asp Arg Cys Glu Ile Cys Cys Asn Pro Ala Cys Phe Gly Cys Leu 1 5 10 15 Asn 16 16 PRT Yersinia enterocolitica 16 Asp Trp Asp Cys Cys Asp Val Cys Cys Asn Pro Ala Cys Ala Gly Cys 1 5 10 15 17 16 PRT Yersinia enterocolitica 17 Asp Trp Asp Cys Cys Asp Val Cys Cys Asn Pro Ala Cys Ala Gly Cys 1 5 10 15 18 17 PRT Yersinia enterocolitica 18 Asn Asp Asp Trp Cys Cys Glu Val Cys Cys Asn Pro Ala Cys Ala Gly 1 5 10 15 Cys 19 16 PRT Yersinia enterocolitica 19 Trp Asp Trp Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Phe Gly Cys 1 5 10 15 20 72 PRT Escherichia coli 20 Met Lys Lys Leu Met Leu Ala Ile Phe Ile Ser Val Leu Ser Phe Pro 1 5 10 15 Ser Phe Ser Gln Ser Thr Glu Ser Leu Asp Ser Ser Lys Glu Lys Ile 20 25 30 Thr Leu Glu Thr Lys Lys Cys Asp Val Val Lys Asn Asn Ser Glu Lys 35 40 45 Lys Ser Glu Asn Met Asn Asn Thr Phe Tyr Cys Cys Glu Leu Cys Cys 50 55 60 Asn Pro Ala Cys Ala Gly Cys Tyr 65 70 21 72 PRT Escherichia coli 21 Met Lys Lys Ser Ile Leu Phe Ile Phe Leu Ser Val Leu Ser Phe Ser 1 5 10 15 Pro Phe Ala Gln Asp Ala Lys Pro Val Glu Ser Ser Lys Glu Lys Ile 20 25 30 Thr Leu Glu Ser Lys Lys Cys Asn Ile Ala Lys Lys Ser Asn Lys Ser 35 40 45 Gly Pro Glu Ser Met Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys 50 55 60 Asn Pro Ala Cys Thr Gly Cys Tyr 65 70 22 71 PRT Yersinia enterocolitica 22 Met Lys Lys Ile Val Phe Val Leu Val Leu Met Leu Ser Ser Phe Gly 1 5 10 15 Ala Phe Gly Gln Glu Thr Val Ser Gly Gln Phe Ser Asp Ala Leu Ser 20 25 30 Thr Pro Ile Thr Ala Glu Val Tyr Lys Gln Ala Cys Asp Pro Pro Leu 35 40 45 Pro Pro Ala Glu Val Ser Ser Asp Trp Asp Cys Cys Asp Val Cys Cys 50 55 60 Asn Pro Ala Cys Ala Gly Cys 65 70 23 54 PRT Artificial Sequence Synthetically generated amino terminal leader sequence 23 Met Lys Lys Ser Ile Leu Phe Ile Phe Leu Ser Val Leu Ser Phe Ser 1 5 10 15 Pro Phe Ala Gln Asp Ala Lys Pro Val Glu Ser Ser Lys Glu Lys Ile 20 25 30 Thr Leu Glu Ser Lys Lys Cys Asn Ile Ala Lys Lys Ser Asn Lys Ser 35 40 45 Gly Pro Glu Ser Met Asn 50 24 53 PRT Artificial Sequence Synthetically generated peptide 24 Met Lys Lys Ser Ile Leu Phe Ile Phe Leu Ser Val Leu Ser Phe Ser 1 5 10 15 Pro Phe Ala Gln Asp Ala Lys Pro Ala Gly Ser Ser Lys Glu Lys Ile 20 25 30 Thr Leu Glu Ser Lys Lys Cys Asn Ile Val Lys Lys Ser Asn Lys Ser 35 40 45 Gly Pro Glu Ser Met 50 25 53 PRT Escherichia coli 25 Met Lys Lys Ser Ile Leu Phe Ile Phe Leu Ser Val Leu Ser Phe Ser 1 5 10 15 Pro Phe Ala Gln Asp Ala Lys Pro Ala Gly Ser Ser Lys Glu Lys Ile 20 25 30 Thr Leu Glu Ser Lys Lys Cys Asn Ile Val Lys Lys Asn Asn Glu Ser 35 40 45 Ser Pro Glu Ser Met 50 26 19 PRT Artificial Sequence Syntheticaly generated peptide 26 Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 27 19 PRT Artificial Sequence Syntheticaly generated peptide 27 Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Trp 1 5 10 15 Gly Cys Tyr 28 19 PRT Artificial Sequence Syntheticaly generated peptide 28 Asn Ser Ser Asn Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 29 14 PRT Artificial Sequence Syntheticaly generated peptide 29 Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 30 14 PRT Artificial Sequence Syntheticaly generated peptide 30 Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Trp Gly Cys Tyr 1 5 10 31 14 PRT Artificial Sequence Syntheticaly generated peptide 31 Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 32 15 PRT Artificial Sequence Syntheticaly generated peptide 32 Asn Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 15 33 15 PRT Artificial Sequence Syntheticaly generated peptide 33 Asn Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Trp Gly Cys Tyr 1 5 10 15 34 15 PRT Artificial Sequence Syntheticaly generated peptide 34 Asn Cys Cys Glu Phe Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 15 35 15 PRT Artificial Sequence Syntheticaly generated peptide 35 Asn Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 15 36 15 PRT Artificial Sequence Syntheticaly generated peptide 36 Asn Cys Cys Glu Trp Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 15 37 15 PRT Artificial Sequence Syntheticaly generated peptide 37 Asn Cys Cys Glu Arg Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 15 38 15 PRT Artificial Sequence Syntheticaly generated peptide 38 Asn Cys Cys Glu Lys Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 15 39 21 PRT Artificial Sequence Syntheticaly generated peptide 39 Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr Asp Phe 20 40 21 PRT Artificial Sequence Syntheticaly generated peptide 40 Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Trp 1 5 10 15 Gly Cys Tyr Asp Phe 20 41 21 PRT Artificial Sequence Syntheticaly generated peptide 41 Asn Ser Ser Asn Tyr Cys Cys Glu Phe Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr Asp Phe 20 42 21 PRT Artificial Sequence Syntheticaly generated peptide 42 Asn Ser Ser Asn Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr Asp Phe 20 43 21 PRT Artificial Sequence Syntheticaly generated peptide 43 Asn Ser Ser Asn Tyr Cys Cys Glu Trp Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr Asp Phe 20 44 21 PRT Artificial Sequence Syntheticaly generated peptide 44 Asn Ser Ser Asn Tyr Cys Cys Glu Arg Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr Asp Phe 20 45 21 PRT Artificial Sequence Syntheticaly generated peptide 45 Asn Ser Ser Asn Tyr Cys Cys Glu Lys Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr Asp Phe 20 46 16 PRT Artificial Sequence Syntheticaly generated peptide 46 Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe 1 5 10 15 47 16 PRT Artificial Sequence Syntheticaly generated peptide 47 Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Trp Gly Cys Tyr Asp Phe 1 5 10 15 48 16 PRT Artificial Sequence Syntheticaly generated peptide 48 Cys Cys Glu Phe Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe 1 5 10 15 49 16 PRT Artificial Sequence Syntheticaly generated peptide 49 Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe 1 5 10 15 50 16 PRT Artificial Sequence Syntheticaly generated peptide 50 Cys Cys Glu Trp Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe 1 5 10 15 51 16 PRT Artificial Sequence Syntheticaly generated peptide 51 Cys Cys Glu Arg Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe 1 5 10 15 52 16 PRT Artificial Sequence Syntheticaly generated peptide 52 Cys Cys Glu Lys Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe 1 5 10 15 53 17 PRT Artificial Sequence Syntheticaly generated peptide 53 Asn Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp 1 5 10 15 Phe 54 17 PRT Artificial Sequence Syntheticaly generated peptide 54 Asn Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Trp Gly Cys Tyr Asp 1 5 10 15 Phe 55 17 PRT Artificial Sequence Syntheticaly generated peptide 55 Asn Cys Cys Glu Phe Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp 1 5 10 15 Phe 56 17 PRT Artificial Sequence Syntheticaly generated peptide 56 Asn Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp 1 5 10 15 Phe 57 17 PRT Artificial Sequence Syntheticaly generated peptide 57 Asn Cys Cys Glu Trp Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp 1 5 10 15 Phe 58 17 PRT Artificial Sequence Syntheticaly generated peptide 58 Asn Cys Cys Glu Arg Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp 1 5 10 15 Phe 59 17 PRT Artificial Sequence Syntheticaly generated peptide 59 Asn Cys Cys Glu Lys Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp 1 5 10 15 Phe 60 42 DNA Artificial Sequence Synthetically generated oligonucleotide 60 cacaccatat gaagaaatca atattattta tttttctttc tg 42 61 46 DNA Artificial Sequence Synthetically generated oligonucleotide 61 cacacctcga gttaggtctc catgctttca ggaccacttt tattac 46 62 69 DNA Artificial Sequence Synthetically generated oligonucleotide 62 gcatgaatag tagcaattac tgctgtgaat tgtgttgtaa tcctgcttgt accgggtgct 60 attaataac 69 63 69 DNA Artificial Sequence Synthetically generated oligonucleotide 63 tcgagttatt aatagcaccc ggtacaagca ggattacaac acaattcaca gcagtaattg 60 ctactattc 69 64 69 DNA Artificial Sequence Synthetically generated oligonucleotide 64 gcatgaatag tagcaattac tgctgtgaat attgttgtaa tcctgcttgt accgggtgct 60 attaataac 69 65 69 DNA Artificial Sequence Synthetically generated oligonucleotide 65 tcgagttatt aatagcaccc ggtacaagca ggattacaac aatattcaca gcagtaattg 60 ctactattc 69 66 21 PRT Artificial Sequence Synthetically generated peptide 66 Xaa Xaa Xaa Xaa Xaa Cys Cys Glu Xaa Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr Xaa Xaa 20 67 19 PRT Artificial Sequence Synthetically generated peptide 67 Gln Ser Ser Asn Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 68 19 PRT Artificial Sequence Synthetically generated peptide 68 Asn Thr Ser Asn Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 69 19 PRT Artificial Sequence Synthetically generated peptide 69 Asn Leu Ser Asn Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 70 19 PRT Artificial Sequence Synthetically generated peptide 70 Asn Ile Ser Asn Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 71 19 PRT Artificial Sequence Synthetically generated peptide 71 Asn Ser Ser Gln Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 72 18 PRT Artificial Sequence Synthetically generated peptide 72 Ser Ser Asn Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly 1 5 10 15 Cys Tyr 73 19 PRT Artificial Sequence Synthetically generated peptide 73 Gln Ser Ser Gln Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 74 18 PRT Artificial Sequence Synthetically generated peptide 74 Ser Ser Gln Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly 1 5 10 15 Cys Tyr 75 19 PRT Artificial Sequence Synthetically generated peptide 75 Asn Ser Ser Asn Tyr Cys Cys Glu Ala Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 76 19 PRT Artificial Sequence Synthetically generated peptide 76 Asn Ser Ser Asn Tyr Cys Cys Glu Arg Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 77 19 PRT Artificial Sequence Synthetically generated peptide 77 Asn Ser Ser Asn Tyr Cys Cys Glu Asn Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 78 19 PRT Artificial Sequence Synthetically generated peptide 78 Asn Ser Ser Asn Tyr Cys Cys Glu Asp Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 79 19 PRT Artificial Sequence Synthetically generated peptide 79 Asn Ser Ser Asn Tyr Cys Cys Glu Cys Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 80 19 PRT Artificial Sequence Synthetically generated peptide 80 Asn Ser Ser Asn Tyr Cys Cys Glu Gln Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 81 19 PRT Artificial Sequence Synthetically generated peptide 81 Asn Ser Ser Asn Tyr Cys Cys Glu Glu Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 82 19 PRT Artificial Sequence Synthetically generated peptide 82 Asn Ser Ser Asn Tyr Cys Cys Glu Gly Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 83 19 PRT Artificial Sequence Synthetically generated peptide 83 Asn Ser Ser Asn Tyr Cys Cys Glu His Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 84 19 PRT Artificial Sequence Synthetically generated peptide 84 Asn Ser Ser Asn Tyr Cys Cys Glu Ile Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 85 19 PRT Artificial Sequence Synthetically generated peptide 85 Asn Ser Ser Asn Tyr Cys Cys Glu Lys Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 86 19 PRT Artificial Sequence Synthetically generated peptide 86 Asn Ser Ser Asn Tyr Cys Cys Glu Met Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 87 19 PRT Artificial Sequence Synthetically generated peptide 87 Asn Ser Ser Asn Tyr Cys Cys Glu Phe Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 88 19 PRT Artificial Sequence Synthetically generated peptide 88 Asn Ser Ser Asn Tyr Cys Cys Glu Pro Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 89 19 PRT Artificial Sequence Synthetically generated peptide 89 Asn Ser Ser Asn Tyr Cys Cys Glu Ser Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 90 19 PRT Artificial Sequence Synthetically generated peptide 90 Asn Ser Ser Asn Tyr Cys Cys Glu Thr Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 91 19 PRT Artificial Sequence Synthetically generated peptide 91 Asn Ser Ser Asn Tyr Cys Cys Glu Trp Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 92 19 PRT Artificial Sequence Synthetically generated peptide 92 Asn Ser Ser Asn Tyr Cys Cys Glu Val Cys Cys Asn Pro Ala Cys Thr 1 5 10 15 Gly Cys Tyr 93 14 PRT Artificial Sequence Synthetically generated peptide 93 Cys Cys Glu Ala Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 94 14 PRT Artificial Sequence Synthetically generated peptide 94 Cys Cys Glu Arg Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 95 14 PRT Artificial Sequence Synthetically generated peptide 95 Cys Cys Glu Asn Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 96 14 PRT Artificial Sequence Synthetically generated peptide 96 Cys Cys Glu Asp Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 97 14 PRT Artificial Sequence Synthetically generated peptide 97 Cys Cys Glu Cys Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 98 14 PRT Artificial Sequence Synthetically generated peptide 98 Cys Cys Glu Gln Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 99 14 PRT Artificial Sequence Synthetically generated peptide 99 Cys Cys Glu Glu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 100 14 PRT Artificial Sequence Synthetically generated peptide 100 Cys Cys Glu Gly Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 101 14 PRT Artificial Sequence Synthetically generated peptide 101 Cys Cys Glu His Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 102 14 PRT Artificial Sequence Synthetically generated peptide 102 Cys Cys Glu Ile Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 103 14 PRT Artificial Sequence Synthetically generated peptide 103 Cys Cys Glu Lys Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 104 14 PRT Artificial Sequence Synthetically generated peptide 104 Cys Cys Glu Met Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 105 14 PRT Artificial Sequence Synthetically generated peptide 105 Cys Cys Glu Phe Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 106 14 PRT Artificial Sequence Synthetically generated peptide 106 Cys Cys Glu Pro Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 107 14 PRT Artificial Sequence Synthetically generated peptide 107 Cys Cys Glu Ser Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 108 14 PRT Artificial Sequence Synthetically generated peptide 108 Cys Cys Glu Thr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 109 14 PRT Artificial Sequence Synthetically generated peptide 109 Cys Cys Glu Trp Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 110 14 PRT Artificial Sequence Synthetically generated peptide 110 Cys Cys Glu Val Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr 1 5 10 111 5 PRT Artificial Sequence Synthetically generated peptide 111 Gln His Asn Pro Arg 1 5 112 6 PRT Artificial Sequence Synthetically generated peptide 112 Val Gln His Asn Pro Arg 1 5 113 7 PRT Artificial Sequence Synthetically generated peptide 113 Val Arg Gln His Asn Pro Arg 1 5 114 8 PRT Artificial Sequence Synthetically generated peptide 114 Val Arg Gly Gln His Asn Pro Arg 1 5 115 9 PRT Artificial Sequence Synthetically generated peptide 115 Val Arg Gly Pro Gln His Asn Pro Arg 1 5 116 10 PRT Artificial Sequence Synthetically generated peptide 116 Val Arg Gly Pro Arg Gln His Asn Pro Arg 1 5 10 117 11 PRT Artificial Sequence Synthetically generated peptide 117 Val Arg Gly Pro Arg Arg Gln His Asn Pro Arg 1 5 10 118 6 PRT Artificial Sequence Synthetically generated peptide 118 Arg Gln His Asn Pro Arg 1 5 119 21 PRT Artificial Sequence Synthetically generated peptide 119 Xaa Xaa Xaa Xaa Xaa Cys Cys Xaa Xaa Cys Cys Xaa Xaa Xaa Cys Xaa 1 5 10 15 Xaa Cys Xaa Xaa Xaa 20 120 21 PRT Artificial Sequence Synthetically generated peptide 120 Xaa Xaa Xaa Xaa Xaa Cys Cys Xaa Xaa Cys Cys Asn Pro Ala Cys Xaa 1 5 10 15 Gly Cys Xaa Xaa Xaa 20 121 5 PRT Artificial Sequence Synthetically generated peptide 121 Asn Ser Ser Asn Tyr 1 5 122 30 PRT Yersinia enterocolitica 122 Gln Ala Cys Asp Pro Pro Leu Pro Pro Ala Glu Val Ser Ser Asp Trp 1 5 10 15 Asp Cys Cys Asp Val Cys Cys Asn Pro Ala Cys Ala Gly Cys 20 25 30 123 6 PRT Artificial Sequence Synthetically generated peptide 123 Lys Lys Lys Lys Lys Lys 1 5 124 7 PRT Artificial Sequence Synthetically generated peptide 124 Asp Lys Lys Lys Lys Lys Lys 1 5

Claims

1. A purified peptide comprising the amino acid sequence (I): Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅Cys₆Cys₇Xaa₈Xaa₉Cys₁₀Cys₁₁Xaa₁₂Xaa₁₃Xaa₁₄Cys₁₅Xaa₁₆Xaa₁₇Cys₁₈Xaa₁Xaa₂₀Xaa₂₁(SEQ ID NO:119) wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is Asn Ser Ser Asn Tyr (SEQ ID NO:121 )or is missing or Xaa₁Xaa₂Xaa₃Xaa₄is missing.

2. The purified peptide of claim 1 wherein Xaa₅is Asn, Trp, Tyr, Asp, or Phe.

3. The purified peptide of claim 1 wherein Xaa₅is Thr or Ile.

4. The purified peptide of claim 1 wherein Xaa₅is Tyr, Asp or Trp.

5. The purified peptide of claim 1 wherein Xaa₈is Glu, Asp, Gln, Gly or Pro.

6. The purified peptide of claim 1 wherein Xaa₉is Leu, Ile, Val, Ala, Lys, Arg, Trp, Tyr or Phe.

7. The purified peptide of claim 1 wherein Xaa₉is Leu, Ile, Val, Lys, Arg, Trp, Tyr or Phe.

8. The purified peptide of claim 1 wherein Xaa₁₂is Asn, Tyr, Asp or Ala.

9. The purified peptide of claim 1 wherein Xaa₁₃is Ala, Pro or Gly.

10. The purified peptide of claim 1 wherein Xaa₁₄is Ala, Leu, Ser, Gly, Val, Glu, Gln, Ile, Leu, Lys, Arg, or Asp.

11. The purified peptide of claim 1 wherein Xaa₁₆is Thr, Ala, Asn, Lys, Arg, Trp.

12. The purified peptide of claim 1 wherein Xaa₁₇is Gly, Pro or Ala.

13. The purified peptide of claim 1 wherein Xaa₁₉is Trp, Tyr, Phe, Asn or Leu.

14. The purified peptide of claim 1 wherein Xaa₁₉is Lys or Arg.

15. The purified peptide of claim 1 wherein Xaa₂₀Xaa₂₁is AspPhe or Xaa₂₀is Asn or Glu and Xaa₂₁is missing.

16. A purified peptide comprising the amino acid sequence:

Asn Ser Ser Asn Tyr Cys Cys (SEQ ID NO:[[---]]28; Glu Tyr Cys Cys Asn Pro Ala MD-915) Cys Thr Gly Cys Tyr.

17. A purified peptide comprising the amino acid sequence:

Cys Cys Glu Tyr Cys Cys Asn (SEQ ID NO:[[--]]31; Pro Ala Cys Thr Gly Cys Tyr. MD-1100)

18. A purified peptide consisting of the amino acid sequence:

Asn Ser Ser Asn Tyr Cys Cys (SEQ ID NO:[[--]]28 Glu Tyr Cys Cys Asn Pro Ala MD-915) Cys Thr Gly Cys Tyr.

19. A purified peptide consisting of the amino acid sequence:

20. A method for treating a gastrointestinal disorder in a patient comprising administering a purified peptide comprising the amino acid sequence:

Asn Ser Ser Asn Tyr Cys Cys [SEQ ID NO:[[--]]26; Glu Leu Cys Cys Asn Pro Ala MM-416776) Cys Thr Gly Cys Tyr.

21. A method for treating a gastrointestinal disorder in a patient comprising administering a purified peptide comprising the amino acid sequence:

22. A method for treating a gastrointestinal disorder in a patient comprising administering a purified peptide comprising the amino acid sequence:

Cys Cys Glu Leu Cys Cys Asn (SEQ ID NO:[[--]]29; Pro Ala Cys Thr Gly Cys Tyr MM416774)

23. A method for treating a gastrointestinal disorder in a patient comprising administering a purified peptide comprising the amino acid sequence:

Cys Cys Glu Tyr Cys Cys Asn (SEQ ID NO:[[--]]29; Pro Ala Cys Thr Gly Cys Tyr. MD-1100)

24. A purified polypeptide comprising an amino acid sequence of any of:

Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Trp Gly Cys Tyr; (SEQ ID NO:[[--]]27) Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Trp Gly Cys Tyr; (SEQ ID NO:[---]30) Asn Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:[[---]32) Asn Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Trp Gly Cys Tyr; (SEQ ID NO:[---]33) Asn Cys Cys Glu Phe Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:[---]34) Asn Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:[---]35) Asn Cys Cys Glu Trp Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:[---]36) Asn Cys Cys Glu Arg Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:[---]37) Asn Cys Cys Glu Lys Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:[---]38) Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp (SEQ ID NO:[---]39) Phe; Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Trp Gly Cys Tyr Asp (SEQ ID NO:[---]40) Phe; Asn Ser Ser Asn Tyr Cys Cys Glu Phe Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp (SEQ ID NO:[---]41) Phe; Asn Ser Ser Asn Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp (SEQ ID NO:[---]42) Phe; Asn Ser Ser Asn Tyr Cys Cys Glu Trp Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp (SEQ ID NO:[---]43) Phe; Asn Ser Ser Asn Tyr Cys Cys Glu Arg Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp (SEQ ID NO:[---]44) Phe; Asn Ser Ser Asn Tyr Cys Cys Glu Lys Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp (SEQ ID NO:[---]45) Phe; Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe; (SEQ ID NO:[---]46) Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Trp Gly Cys Tyr Asp Phe; (SEQ ID NO:[---]47) Cys Cys Glu Phe Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe; (SEQ ID NO:[---]48) Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe; (SEQ ID NO:[---]49) Cys Cys Glu Trp Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe; (SEQ ID NO:[---]50) Cys Cys Glu Arg Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe; (SEQ ID NO:[---]51) Cys Cys Glu Lys Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe; (SEQ ID NO:[--]52) Asn Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe; (SEQ ID NO:[--])53 Asn Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Trp Gly Cys Tyr Asp Phe; (SEQ ID NO: [---])54 Asn Cys Cys Glu Phe Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe; (SEQ ID NO:[---]55) Asn Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe; (SEQ ID NO:[---]56) Asn Cys Cys Glu Trp Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe; (SEQ ID NO:[---]57) Asn Cys Cys Glu Arg Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe; (SEQ ID NO:[---]58) Asn Cys Cys Glu Lys Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr Asp Phe; (SEQ ID NO:[---]59) Gln Ser Ser Asn Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:[---]67) Asn Thr Ser Asn Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:[---]68) Asn Leu Ser Asn Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:[---]69) Asn Ile Ser Asn Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:[---]70) Asn Ser Ser Gln Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:[---]71) Ser Ser Asn Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:[---]72) Gln Ser Ser Gln Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:[---]73) Ser Ser Gln Tyr Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:[---]74) Asn Ser Ser Asn Tyr Cys Cys Glu Ala Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:75) Asn Ser Ser Asn Tyr Cys Cys Glu Arg Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:76) Asn Ser Ser Asn Tyr Cys Cys Glu Asn Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:77) Asn Ser Ser Asn Tyr Cys Cys Glu Asp Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:78) Asn Ser Ser Asn Tyr Cys Cys Glu Cys Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:79) Asn Ser Ser Asn Tyr Cys Cys Glu Gln Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:80) Asn Ser Ser Asn Tyr Cys Cys Glu Glu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:81) Asn Ser Ser Asn Tyr Cys Cys Glu Gly Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:82) Asn Ser Ser Asn Tyr Cys Cys Glu His Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:83) Asn Ser Ser Asn Tyr Cys Cys Glu Ile Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:84) Asn Ser Ser Asn Tyr Cys Cys Glu Lys Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:85) Asn Ser Ser Asn Tyr Cys Cys Glu Met Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:86) Asn Ser Ser Asn Tyr Cys Cys Glu Phe Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:87) Asn Ser Ser Asn Tyr Cys Cys Glu Pro Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:88) Asn Ser Ser Asn Tyr Cys Cys Glu Ser Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:89) Asn Ser Ser Asn Tyr Cys Cys Glu Thr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:90) Asn Ser Ser Asn Tyr Cys Cys Glu Trp Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:91) Asn Ser Ser Asn Tyr Cys Cys Glu Val Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:92) Cys Cys Glu Ala Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:93) Cys Cys Glu Arg Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:94) Cys Cys Glu Asn Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:95) Cys Cys Glu Asp Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:96) Cys Cys Glu Cys Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:97) Cys Cys Glu Gln Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:98) Cys Cys Glu Glu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:99) Cys Cys Glu Gly Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:100) Cys Cys Glu His Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:101) Cys Cys Glu Ile Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:102) Cys Cys Glu Lys Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:103) Cys Cys Glu Met Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:104) Cys Cys Glu Phe Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:105) Cys Cys Glu Pro Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:106) Cys Cys Glu Ser Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:107) Cys Cys Glu Thr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:108) Cys Cys Glu Trp Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:109) Cys Cys Glu Val Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:110) Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr; (SEQ ID NO:[--]26; and MM-416776) Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr. (SEQ ID NO:[--]29; MM416774)

25. A method for treating a gastrointestinal disorder in a patient comprising administering the peptide of claim 1.

26. A method for treating a gastrointestinal disorder in a patient comprising administering the peptide of claim 24.

27. The method of any of claims 20-26 wherein the gastrointestinal disorder is a gastrointestinal motility disorder.

28. The method of any of claims 20-26 wherein the gastrointestinal disorder is selected from the group consisting of a gastrointestinal motility disorder, irritable bowel syndrome, chronic constipation, a functional gastrointestinal disorder, gastroesophageal reflux disease, functional heartburn, dyspepsia, functional dyspepsia, nonulcer dyspepsia, gastroparesis, chronic intestinal pseudo-obstruction, colonic pseudo-obstruction, Crohn's disease, ulcerative colitis, and inflammatory bowel disease.

29. A method for treating obesity comprising administering the peptide of any of claims 1, 16-19, and 24.

30. A method for treating congestive heart failure comprising administering the peptide of any of claims 1, 16-19, and 24.

31. A method for treating benign prostatic hyperplasia comprising administering the peptide of any of claims 1, 16-19, and 24.

32. The purified peptide of any of claims 1, 16, 17, and 24 wherein the polypeptide comprises the amino acid sequence DF; QHNPR (SEQ ID NO:111); VQHNPR (SEQ ID NO:112); VRQHNPR (SEQ ID NO:113); VRGQHNPR (SEQ ID NO:114); VRGPQHNPR (SEQ ID NO:115); VRGPRQHNPR (SEQ ID NO:116); VRGPRRQHNPR (SEQ ID NO:117); or RQHNPR (SEQ ID NO:118) fused to its amino terminus or its caboxy terminus.

33. The purified peptide of any of claims 1, 16, 17, and 24 wherein the purified polypeptide comprises the amino acid sequence of an analgesic peptide selected from the group consisting of endomorphin-1, endomorphin-2, nocistatin, dalargin, lupron, and substance P fused to its amino terminus or its carboxy terminus.

34. The purified peptide of any of claims 1, 16, 17, and 24 wherein the polypeptide includes no more than 10 additional amino acids at its amino terminus or carboxy terminus or both and wherein the polypeptide is a guanylate cyclase receptor agonist.

35. The purified peptide of claim 1 wherein wherein: Xaa₁Xaa₂Xaa₃Xaa₄Xaa₅is missing; Xaa₈is Glu; Xaa₉is Leu, Ile, Lys, Arg, Trp, Tyr or Phe; Xaa₁₂is Asn; Xaa₁₃is Pro; Xaa₁₄is Ala; Xaa₁₆is Thr, Ala, Lys, Arg, Trp; Xaa₁₇is Gly; Xaa₁₉is Tyr or Leu; and Xaa₂₀Xaa₂₁is AspPhe or is missing.

36. A method for treating a patient suffering from constipation, the method comprising administering the polypeptide of any of claims 1, 16-23 and 28.

37. A method for increasing the activity of an intestinal guanylate cyclase (GC—C) receptor in a patient, the method comprising administering the polypeptide of any of claims 1, 16-19 and 24.

38. A method for treating a gastrointestinal disorder in a patient comprising administering a GC—C receptor agonist.

39. The method of claim 38 wherein the gastrointestinal disorder is a gastrointestinal motility disorder.

40. The method of claim 38 wherein the gastrointestinal disorder is selected from the group consisting of a gastrointestinal motility disorder, irritable bowel syndrome, chronic constipation, a functional gastrointestinal disorder, gastroesophageal reflux disease, functional heartburn, dyspepsia, functional dyspepsia, nonulcer dyspepsia, gastroparesis, chronic intestinal pseudo-obstruction, colonic pseudo-obstruction, Crohn's disease, ulcerative colitis, and inflammatory bowel disease.

41. A method for treating obesity comprising administering a GC—C receptor agonist.

42. A method for treating congestive heart failure comprising administering a GC—C receptor agonist.

43. A method for treating benign prostatic hyperplasia comprising administering a GC—C receptor agonist.

44. A method for treating visceral pain comprising administering a GC—C receptor agonist.

45. A method for treating inflammation comprising administering a GC—C receptor agonist.

46. A method for treating constipation comprising administering a GC—C receptor agonist.

47. A method for treating visceral pain comprising administering the polypeptide of any of claims 1, 16-19 and 24.

48. A method for treating inflammation comprising administering the polypeptide of any of claims 1, 16-19 and 24.

49. A method for treating cystic fibrosis comprising administering the polypeptide of any of claims 1, 16-19 and 24.

50. A method for treating cystic fibrosis comprising administering a GC—C receptor agonist.

51. A pharmaceutical composition comprising the peptide of any of claims 1, 16-19 and 24 and a pharmaceutically acceptable carrier.

52. A pharmaceutical composition comprising the peptide of any of claims 1, 16-19 and 24 surrounded by an enteric coating.

53. A controlled release pharmaceutical composition comprising the peptide of any of claims 1, 16-19 and 24 and a biodegradable polymeric matrix.

54. A pharmaceutical composition comprising the peptide of any of claims 1, 16-19 and 24, an analgesic agent and a pharmaceutically acceptable carrier.

55. A pharmaceutical composition comprising the peptide of any of claims 1, 16-19 and 24, a phosphodiesterase inhibitor and a pharmaceutically acceptable carrier.

56. A method for treating cancer, a respiratory disorder, a neurological disorder, a disorder associated with fluid and sodium retention, a disorder associated with carbonate imbalance, erectile dysfunction, an insulin-related disorder, or an inner ear disorder, the method comprising administering the peptide of any of claims 1, 16-19 and 24.

57. A method for treating cancer, a respiratory disorder, a neurological disorder, a disorder associated with fluid and sodium retention, a disorder associated with carbonate imbalance, erectile dysfunction, an insulin-related disorder, or an inner ear disorder, the method comprising administering a GC—C receptor agonist.

58. A method of producing the peptide of any of claims 16-19 and 24, comprising providing a cell harboring a nucleic acid molecule encoding the polypeptide, culturing the cell under conditions in which the peptide is expressed, and isolating the expressed peptide.

59. A method of producing the peptide of any of claims 16-19 and 24, comprising chemically synthesizing the peptide and they purifying the synthesized peptide.

60. A pharmaceutical composition comprising the peptide of any of claims 1, 16-19 and 24 and a natriuretic peptide such as atrial natriuretic peptide, brain natriuretic peptide, a C-type natriuretic peptide, a diuretic, or an inhibitor of angiotensin converting enzyme.

61. A pharmaceutical composition comprising the peptide of any of claims 1, 16-19 and 24 and a 5-alpha reductase inhibitor or an alpha adrenergic inhibitor.

62. A pharmaceutical composition comprising the peptide of any of claims 1, 16-19 and 24 and gut hormone fragment peptide YY_3-36, glp-1 (glucagon-like peptide-1), exendin-4 (an inhibitor of glp-1), sibutramine, phentermine, phendimetrazine, benzphetamine hydrochloride (Didrex), orlistat (Xenical), diethylpropion hydrochloride (Tenuate), fluoxetine (Prozac), bupropion, ephedra, chromium, garcinia cambogia, benzocaine, bladderwrack (focus vesiculosus), chitosan, nomame herba, galega (Goat's Rue, French Lilac), conjugated linoleic acid, L-carnitine, fiber (psyllium, plantago, guar fiber), caffeine, dehydroepiandrosterone, germander (teucrium chamaedrys), B-hydroxy-β-methylbutyrate, or pyruvate.

63. A pharmaceutical composition comprising a GC—C receptor agonist and a natriuretic peptide such as atrial natriuretic peptide, brain natriuretic peptide, a C-type natriuretic peptide, a diuretic, or an inhibitor of angiotensin converting enzyme.

64. A pharmaceutical composition comprising a GC—C receptor agonist and a 5-alpha reductase inhibitor or an alpha adrenergic inhibitor.

65. A pharmaceutical composition comprising a GC—C receptor agonist and gut hormone fragment peptide YY_3-36, glp-1 (glucagon-like peptide-1), exendin-4 (an inhibitor of glp-1), sibutramine, phentermine, phendimetrazine, benzphetamine hydrochloride (Didrex), orlistat (Xenical), diethylpropion hydrochloride (Tenuate), fluoxetine (Prozac), bupropion, ephedra, chromium, garcinia cambogia, benzocaine, bladderwrack (focus vesiculosus), chitosan, nomame herba, galega (Goat's Rue, French Lilac), conjugated linoleic acid, L-carnitine, fiber (psyllium, plantago, guar fiber), caffeine, dehydroepiandrosterone, germander (teucrium chamaedrys), B-hydroxy-,-methylbutyrate, or pyruvate.

66. A method for treating congestive heart failure comprising administering the peptide of any of claims 1, 16-19 and 24 and a natriuretic peptide such as atrial natriuretic peptide, brain natriuretic peptide, a C-type natriuretic peptide, a diuretic, or an inhibitor of angiotensin converting enzyme.

67. A method for treating benign prostatic hyperplasia comprising administering the peptide of any of claims 1, 16-19 and 24 and a 5-alpha reductase inhibitor or an alpha adrenergic inhibitor.

68. A method for treating obesity comprising administering the peptide of any of claims 1, 16-19 and 24 and gut hormone fragment peptide YY_3-36, glp-1 (glucagon-like peptide-1), exendin-4 (an inhibitor of glp-1), sibutramine, phentermine, phendimetrazine, benzphetamine hydrochloride (Didrex), orlistat (Xenical), diethylpropion hydrochloride (Tenuate), fluoxetine (Prozac), bupropion, ephedra, chromium, garcinia cambogia, benzocaine, bladderwrack (focus vesiculosus), chitosan, nomame herba, galega (Goat's Rue, French Lilac), conjugated linoleic acid, L-carnitine, fiber (psyllium, plantago, guar fiber), caffeine, dehydroepiandrosterone, germander (teucrium chamaedrys), B-hydroxy-β-methylbutyrate, or pyruvate.

69. A method for treating congestive heart failure comprising administering a GC—C receptor agonist and a natriuretic peptide such as atrial natriuretic peptide, brain natriuretic peptide, a C-type natriuretic peptide, a diuretic, or an inhibitor of angiotensin converting enzyme.

70. A method for treating benign prostatic hyperplasia comprising a GC—C receptor agonist and a 5-alpha reductase inhibitor or an alpha adrenergic inhibitor.

71. A method for treating obesity comprising administering a GC—C receptor agonist and gut hormone fragment peptide YY_3-36, glp-1 (glucagon-like peptide-1), exendin-4 (an inhibitor of glp-1), sibutramine, phentermine, phendimetrazine, benzphetamine hydrochloride (Didrex), orlistat (Xenical), diethylpropion hydrochloride (Tenuate), fluoxetine (Prozac), bupropion, ephedra, chromium, garcinia cambogia, benzocaine, bladderwrack (focus vesiculosus), chitosan, nomame herba, galega (Goat's Rue, French Lilac), conjugated linoleic acid, L-carnitine, fiber (psyllium, plantago, guar fiber), caffeine, dehydroepiandrosterone, germander (teucrium chamaedrys), B-hydroxy-β-methylbutyrate, or pyruvate.