US20020156126A1 - FT-0554 substance and its production - Google Patents
FT-0554 substance and its production Download PDFInfo
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- US20020156126A1 US20020156126A1 US10/120,523 US12052302A US2002156126A1 US 20020156126 A1 US20020156126 A1 US 20020156126A1 US 12052302 A US12052302 A US 12052302A US 2002156126 A1 US2002156126 A1 US 2002156126A1
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- NMEGHQQRWKBPQO-NGKQVELOSA-N CCC(C)/C=C/C=C(\C)CC(C)/C=C/C=C/C1OC(=O)C(O)C2OC12C Chemical compound CCC(C)/C=C/C=C(\C)CC(C)/C=C/C=C/C1OC(=O)C(O)C2OC12C NMEGHQQRWKBPQO-NGKQVELOSA-N 0.000 description 5
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
- C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
- C07D493/04—Ortho-condensed systems
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/10—Anthelmintics
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
- C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin
Definitions
- This invention relates to novel FT-0554 substance useful for treatment for infection of parasite, especially helminth, and its production.
- Anthelmintics used at present such as avermectins, mebendazole and praziquantel, are not always sufficient for satisfactory in usefulness and toxicity, and the anthelmintics, which can solve these problems, are strongly, required.
- the present invention provides novel FT-0554 substance, which can satisfy the above requirements, and its production.
- An object of the present invention is to provide FT-0554 substance shown by the compound of the formula [1]
- Another object of the present invention is to provide a process for production of FT-0554 substance of the formula [1]
- [0008] which comprises culturing a microorganism belonging to fungus having FT-0554 substance producing activity, accumulating FT-0554 substance in the cultured medium and isolating FT-0554 substance from the cultured medium.
- FT-0554 substance producing microorganism is the fungi having FT-0554 substance producing activity and is not limited.
- a microorganism used for production of FT-0554 is a fungus strain FT-0554 isolated from a newly collected sponge by the inventors of the present invention.
- Taxonomical properties of the microorganism are illustrated as follows.
- the microorganism of the present invention shows good growth on Czapek-yeast extract agar medium which contains seawater 50% (salt content 3.4%), malt extract agar medium, Czapek-yeast extract agar medium which contains sucrose 20% and potato-glucose agar medium, with abundance of conidia.
- Plural aspergillae consist of metulae and phialides with the size of 8.4-11.4 ⁇ 2.4-3.4 ⁇ m and 5.4-8.6 ⁇ 2.8-3.3 ⁇ m. respectively.
- Whole of the vesicles is covered with metulae with forming conidial heads segmented from spherical to cylindrical.
- Conidia is globose with a size of diameter 3-4.5 ⁇ m having smooth to rough surface.
- Optimum growth condition of the present strain is pH 5-7, temperature 16-36° C. and seawater concentration 1) 50-100%.
- Growth range of the strain is pH 3-10, temperature 12-45° C. and seawater concentration 2) 0-100%.
- the present microorganism strain has deposited as Aspergillus niger FT-0554 FERM P-16399 in National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, Ministry of International Science and Technology, 1-3, Higashi 1 - chose, Tsukuba-shi, Ibaraki-ken, Japan on Sep. 1, 1997. Further, the present microorganism strain was transferred to the microorganism deposition under Budapest Treaty in National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, Ministry of International Science and Technology, 1-3, Higashi 1-chome, Tsukuba-shi, Ibaraki-ken, Japan on Jul. 31, 1998, and was given deposition No. FERM BP-6443 from the International Deposition Authority.
- Production of FT-0554 substance of the present invention can be performed by culturing microorganism belonging to fungi having producing activity of FT-0554 substance and isolating from the cultured mass and purifying the product.
- the microorganism strain used in the present invention can be the above microorganism strain, its variants and mutants, and all strains having FT-0554 substance producing activity belonging to fungi.
- Nutritional sources for production of the above FT-0554 substance can be a nutritional source for fungi.
- nitrogen sources are commercially available peptone, meat extract, corn steep liquor, cottonseed powder, peanut powder, soybean flour, yeast extract, NZ-amine, casein hydrolyzate, sodium nitrate, ammonium nitrate and ammonium sulfate.
- carbon sources are carbohydrate such as glycerin, starch, glucose, galactose and mannose, or carbon source such as fats and oil, and inorganic salts such as sodium chloride, phosphate, calcium carbonate and magnesium sulfate. These can be used with or combination thereof.
- Trace metallic salt and animal, vegetable or mineral oil as antiform agent can be added if necessary. These are substance, which can be assimilated by the producing strain and are useful for production of FT-0554 substance, can be used. All the known medium for culturing the fungus can be used. Mass production of FT-0554 substance can preferably be performed by a liquid culture. Culturing temperature can be applied within the range of growing the producing microorganism strain and producing FT-0554 substance. Culturing can be performed by selecting suitable conditions depending on the nature of FT-0554 substance producing strain.
- FT-0554 substance can be extracted by water immiscible organic solvent such as chloroform and ethyl acetate from the culture liquid.
- water immiscible organic solvent such as chloroform and ethyl acetate
- known isolation method used for lipophilic substance for example adsorption chromatography, gel filtration chromatography, scratching from thin layer chromatography, centrifugal counter current chromatography, HPLC, and the like with or without combination thereof or repeated operation, can be applied to obtain purified substance.
- FT-0554 substance is determined as the following chemical structure.
- FT-0554 substance As shown in the above, physico-chemical properties of FT-0554 substance are explained in detail, however no compound having identical properties have been reported. Consequently, FT-0554 substance is defined as novel substance.
- NADH-fumarate reductase inhibitory activity of FT-0554 of the present invention is explained as follows.
- Muscles of Ascaris suum were homogenized in 120 mM sodium phosphate solution (pH 7.0) and centrifuged at 3,000 ⁇ g for 10 minutes to collect the supernatant solution. The supernatant was further centrifuged at 10,000 ⁇ g for 20 minutes to collect the precipitate. The precipitate was suspended in 120 mM sodium phosphate solution (pH 7.0) to obtain mitochondrial fraction.
- test sample dissolved in 50% dimethyl sulfoxide solution was added into 96 holes microplate, 120 mM sodium phosphate solution (pH 7.0) containing 0.35 mM NADH. 7.2 mM disodium fumarate and 18 mg/ml bovine serum albumin was added thereto, and pre-incubated in the microplate reader ELx808 (Bio-Tek industries Co.) at 37° C. for 5 minutes.
- Antimicrobial activity of FT-0554 substance of the present invention is as follows.
- Chloroform solution of the compound of the present invention (1 mg/ml) 10 ⁇ l is dipped on a filter paper disk (Advantec Co. diameter 6 mm). which is air-dried to remove the solvent. The air-dried to remove the solvent. The dried disks are put on the agar plates containing test organisms, incubated at 37° C. or 27° C. for 24 hours, and diameter of the inhibition zone around the paper disk is measured. Result are shown in Table 3.
- FT-0554 substance of the present invention has weak growth inhibitory activity against some microorganisms.
- FIG. 1 shows UV spectrum of FT-0554 substance of the present invention in 1-propanol solution (50 ⁇ M).
- FIG. 2 shows IR spectrum of FT-0554 substance of the present invention (KBr Tab.).
- a loopful of Aspergillus niger FT-0554 (FERM BP-6443) cultured in agar slant medium was inoculated into the liquid medium (pH 7.0) containing glucose 2.0%, polypeptone (Nihon Seiyaku Co.) 0.5%, agar 0.1%, yeast extract (Oriental Yeast Co.) 0.2%, magnesium sulfate 7 hydrate 0.05% and pottassium dihydrogen phosphate 0.1% dissolved in 50% natural seawater (salt concentration 3.4% natural seawater was used) and divided into 100 ml in a 500 ml Erlenmeyer flask, and shake cultured at 27° C. for 2 days.
- This seed culture liquid each 1 ml was inoculated into the liquid medium containing potato dextrose broth (Difco Inc.) 2.4% dissolved in 50% natural seawater (salt concentration 3.4% natural seawater was used) divided each 100 ml in a 500 ml Erlenmeyer flask ( ⁇ 30 flasks) and shake cultured at 27° C. for 96 hours.
- potato dextrose broth Difco Inc.
- 50% natural seawater salt concentration 3.4% natural seawater was used
- the crude substance II was charged on a silica gel thin layer plates (Merck Art, 5744) and developed with n-hexane-ethyl acetate (1:1). Active fraction was scratched and eluated with chloroform-methanol (2:1). The eluate was concentrated in vacuo to obtain crude substance III, 13.4 mg. Further the substance III was charged on Sephadex LH-20 column and eluated with chloroform-methanol (1:2). The eluate was concentrated in vacuo to obtain FT-0554 substance as white powder 10.0 mg.
- FT-0554 substance of the present invention is expected to be a satisfactory and useful medicament for treatment of parasitosis.
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Abstract
The present invention is to obtain novel FT-0554 substance which is useful for treatment of helminthiasis. The present invention comprising the steps of culturing the microorganism belonging to fungi having producing activity of FT-0554 substance represented by the following formula [I]
subjected to accumulation of FT-0554 substance in the cultured medium, and isolating FT-0554 substance from the said cultured mass. The medicament useful for treatment of parasitic infection, specifically helminthiasis can be obtained.
Description
- This invention relates to novel FT-0554 substance useful for treatment for infection of parasite, especially helminth, and its production.
- Parasitosis is reducing as a result of improvement in sanitary conditions and progress of anthelmintics. Recently, however, the import parasitosis, zoonotic parasitosis, opportunistic parasitosis and parasitosis originated from perishable foods are prevailing and become crucial problems, Further the parasitosis produces large economical burdens in the stock-farming and agriculture. For infection of helminth in the parasite, at present, avermectins, mebendazole, praziquantel, and others are used for treatment of helminth.
- Anthelmintics used at present, such as avermectins, mebendazole and praziquantel, are not always sufficient for satisfactory in usefulness and toxicity, and the anthelmintics, which can solve these problems, are strongly, required.
- Consequently, the present invention provides novel FT-0554 substance, which can satisfy the above requirements, and its production.
- We have studided NADH-fumarate reductase, which was one of the promising targets against anthelmintics, in the electron transport system of the helminth, and explored for screening in the microbial culture. We have found that novel FT-0554 substance had NADH-fumarate reductase inhibitory activity, and completed the present invention according to this acknowledge.
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- which comprises culturing a microorganism belonging to fungus having FT-0554 substance producing activity, accumulating FT-0554 substance in the cultured medium and isolating FT-0554 substance from the cultured medium.
- Further object of the present invention is to provide a microorganism belonging to fungi and having FT-0554 substance producing activity of the above process beingAspergillus niger FT-0554. Still further object of the present invention is to provide a microorganism belonging to fungi and having FT-0554 substance producing activity being Aspergillus niger FT-0554.
- FT-0554 substance producing microorganism is the fungi having FT-0554 substance producing activity and is not limited. Preferable example of a microorganism used for production of FT-0554 is a fungus strain FT-0554 isolated from a newly collected sponge by the inventors of the present invention.
- Taxonomical properties of the microorganism are illustrated as follows.
- Taxonomical properties of a strain FT-0554.
- (1) Cultured properties on various media
- Results of macroscopic observation of the strain of the present microorganism cultured at 25° C. for 7 days are shown in Table 1.
TABLE 1 Growth condition Color of Color of on the medium surface of reverse side soluble Medium (diameter of colony) colony of colony pigment Czapek-yeast good (82 mm) black brown pale yellow none extract agar velvety, entire Malt extract good (81 mm) black brown pale yellow none agar medium velvety, entire 20% sucrose Czapek-yeast good (82 mm) black brown pale yellow none extract agar medium velvety, entire Potate-glucose good (>85 mm) black brown pale yellow none agar medium velvety, entire Miura agar medium moderate (40 mm) black brown white none velvety, entire - (2) Morphological properties
- The microorganism of the present invention shows good growth on Czapek-yeast extract agar medium which contains seawater 50% (salt content 3.4%), malt extract agar medium, Czapek-yeast extract agar medium which contains sucrose 20% and potato-glucose agar medium, with abundance of conidia.
- Microscopical observation of colonies grown on Czapek-yeast extract agar medium shows transparent hyphae with septa, straight grown conidiophore on the substrate mycelia with length 500 μm-2.5 mm, and foot-cell in the basement. Tops of conidiophores are hypertrophic from spherical to subspherical with forming vesicles of diameter 35-60 μm.
- Plural aspergillae consist of metulae and phialides with the size of 8.4-11.4×2.4-3.4 μm and 5.4-8.6×2.8-3.3 μm. respectively. Whole of the vesicles is covered with metulae with forming conidial heads segmented from spherical to cylindrical. Conidia is globose with a size of diameter 3-4.5 μm having smooth to rough surface.
- (3) Physiological properties
- 1) Optimum growth condition
- Optimum growth condition of the present strain is pH 5-7, temperature 16-36° C. and seawater concentration1)50-100%.
-
- 2) Growth condition
- Growth range of the strain is pH 3-10, temperature 12-45° C. and seawater concentration2) 0-100%.
-
- 3) Nature
- Aerobic
- As shown in the above, culture condition, taxonomical properties and physiological properties of the present microorganism strain FT-0554 are compared with the known microorganism strains. The present strain is identified as belonging toAspergillus niger and referred to Aspergillus niger FT-0554.
- The present microorganism strain has deposited asAspergillus niger FT-0554 FERM P-16399 in National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, Ministry of International Science and Technology, 1-3, Higashi 1 - chose, Tsukuba-shi, Ibaraki-ken, Japan on Sep. 1, 1997. Further, the present microorganism strain was transferred to the microorganism deposition under Budapest Treaty in National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, Ministry of International Science and Technology, 1-3, Higashi 1-chome, Tsukuba-shi, Ibaraki-ken, Japan on Jul. 31, 1998, and was given deposition No. FERM BP-6443 from the International Deposition Authority.
- Production of FT-0554 substance of the present invention can be performed by culturing microorganism belonging to fungi having producing activity of FT-0554 substance and isolating from the cultured mass and purifying the product. The microorganism strain used in the present invention can be the above microorganism strain, its variants and mutants, and all strains having FT-0554 substance producing activity belonging to fungi.
- Nutritional sources for production of the above FT-0554 substance can be a nutritional source for fungi. Examples of nitrogen sources are commercially available peptone, meat extract, corn steep liquor, cottonseed powder, peanut powder, soybean flour, yeast extract, NZ-amine, casein hydrolyzate, sodium nitrate, ammonium nitrate and ammonium sulfate. Example of carbon sources are carbohydrate such as glycerin, starch, glucose, galactose and mannose, or carbon source such as fats and oil, and inorganic salts such as sodium chloride, phosphate, calcium carbonate and magnesium sulfate. These can be used with or combination thereof.
- Trace metallic salt and animal, vegetable or mineral oil as antiform agent can be added if necessary. These are substance, which can be assimilated by the producing strain and are useful for production of FT-0554 substance, can be used. All the known medium for culturing the fungus can be used. Mass production of FT-0554 substance can preferably be performed by a liquid culture. Culturing temperature can be applied within the range of growing the producing microorganism strain and producing FT-0554 substance. Culturing can be performed by selecting suitable conditions depending on the nature of FT-0554 substance producing strain.
- FT-0554 substance can be extracted by water immiscible organic solvent such as chloroform and ethyl acetate from the culture liquid. In addition to the above extraction method, known isolation method used for lipophilic substance, for example adsorption chromatography, gel filtration chromatography, scratching from thin layer chromatography, centrifugal counter current chromatography, HPLC, and the like with or without combination thereof or repeated operation, can be applied to obtain purified substance.
- Physico-chemical properties of FT-0554 substance of the present invention are shown as follows.
- (1) Nature white powder or amorphous
- (2) Molecular weight 361.2374 (M+H, high resolution fast atom bombardment mass spectroscopy)
- (3) Molecular formula C22H32O4
- (4) Specific rotation: [a]D 25=+35.83 (c=0.1, 1-propanol)
- (5) UV absorption maximum (in 1-propanol): As shown in FIG. 1, maximum absorption at 205 nm (shoulder, ε=10800) and 231 nm (ε=21000).
- (6) IR absorption maximum (KBr Tab): As shown in FIG. 2, maximum absorption at 3430, 2960, 2920, 2850, 1740, 1660, 1460, 1400, 1380, 1180, 1120 and 1000 cm−1
- (7)1H-NMR: chemical shift in deuterochloroform (ppm) and spin-spin coupling constant (Hz) in Table 2
- (8)13C NMR: chemical shift in deuterochloroform (ppm) in Table 2
- (9) Solubility in solvent: soluble in chloroform, ethanol, 1-propanol, toluene and ethyl acetate, insoluble in water and n-hexane
- (10) Color reaction: positive for sulfuric acid and iodine.
TABLE 2 13C 1H 170.6 s 145.2 d 5.79 dd (1 H, J = 6.9, 15.2) 138.9 d 5.46 dd (1 H, J = 7.6, 15.2) 137.9 d 6.37 dd (1 H, J = 10.2, 15.2) 133.7 s 127.0 d 5.76 d (1 H, J = 10.9 Hz) 126.0 d 6.02 dd (1 H, J = 10.2, 15.2) 124.6 d 6.17 dd (1 H, J = 10.9, 15.2) 122.0 d 5.48 dd (1 H, J = 7.9, 15.2) 80.2 d 4.92 d (1 H, J = 7.9) 68.0 d 4.57 s (1 H) 58.5 d 3.52 s (1 H) 58.2 s 47.2 t 1.96 dd (1 H, J = 7.7, 13.7) 2.09 dd (1 H, J = 7.1, 13.7) 38.6 d 2.06 m (1 H) 34.8 d 2.42 m (1 H) 29.8 t 1.31 dq (2 H, J = 7.1, 7.3) 20.2 q 0.98 d (3 H, J = 6.8) 19.4 q 0.97 d (3 H, J = 6.8) 17.8 q 1.45 s (3 H) 16.5 q 1.69 s (3 H) 11.8 q 0.85 t (3 H, J = 7.3) - In Table 2, s: singlet, d: doublet, t: triplet, q: quartet, m: multiplet, H: number of proton, and J: spin-spin coupling constant (Hz).
-
- As shown in the above, physico-chemical properties of FT-0554 substance are explained in detail, however no compound having identical properties have been reported. Consequently, FT-0554 substance is defined as novel substance.
- NADH-fumarate reductase inhibitory activity of FT-0554 of the present invention is explained as follows.
- Muscles ofAscaris suum were homogenized in 120 mM sodium phosphate solution (pH 7.0) and centrifuged at 3,000×g for 10 minutes to collect the supernatant solution. The supernatant was further centrifuged at 10,000×g for 20 minutes to collect the precipitate. The precipitate was suspended in 120 mM sodium phosphate solution (pH 7.0) to obtain mitochondrial fraction.
- After 10 μl of test sample dissolved in 50% dimethyl sulfoxide solution was added into 96 holes microplate, 120 mM sodium phosphate solution (pH 7.0) containing 0.35 mM NADH. 7.2 mM disodium fumarate and 18 mg/ml bovine serum albumin was added thereto, and pre-incubated in the microplate reader ELx808 (Bio-Tek industries Co.) at 37° C. for 5 minutes.
- Mitochondrial fraction ofAscaris suum 10 μl (protein content 0.3 mg) was added therein and incubated at 37° C. for 10 minutes. Absorption of NADH at 340 nm was measured every 15 seconds. As a result of quantitative measurement of NADH-fumarate reductase activity shown by decrease in the slope of absorbancy at 340 nm, 50% inhibition of NADH-fumarate reductase activity were obtained at 2.8 μM of FT-0554 substance. Consequently, FT-0554 substance can be expected to use as drug for treatment or prevention of helminthiasis.
- Antimicrobial activity of FT-0554 substance of the present invention is as follows.
- Chloroform solution of the compound of the present invention (1 mg/ml) 10 μl is dipped on a filter paper disk (Advantec Co. diameter 6 mm). which is air-dried to remove the solvent. The air-dried to remove the solvent. The dried disks are put on the agar plates containing test organisms, incubated at 37° C. or 27° C. for 24 hours, and diameter of the inhibition zone around the paper disk is measured. Result are shown in Table 3.
TABLE 3 inhibition zone Test organisms diameter (mm) Escherichia coli KB213 (NIHJ) − Escherichia coli KB176 (NIHJ JC-2, IFO 12734) − Pseudomonas aeruginosa P-3 KB105 + Xanthomonas oryzae KB88 − Micrococcus luteus KB40 (PCI 1001) − Staphylococcus aureus KB210 − Mycobacterium smegmatis KB42 (ATCC 607) − Bacillus subtilis KB27 (PCI 219) − Bacteroides fragilis KB169 (ATCC 23745) − Acholeplasma laidrawii KB174 − Candida albicans KF1 12 Saccharomyces cervisiae KF26 − Aspergillus niger KF103 (ATCC 6275) − Pyricularia oryzae KF180 ± Mucor racemosus KF223 (IFO 4581) 10 - As shown in Table 3, FT-0554 substance of the present invention has weak growth inhibitory activity against some microorganisms.
- FIG. 1 shows UV spectrum of FT-0554 substance of the present invention in 1-propanol solution (50 μM).
- FIG. 2 shows IR spectrum of FT-0554 substance of the present invention (KBr Tab.).
- The following examples illustrate the present invention, but is not construed to limit the invention.
- A loopful ofAspergillus niger FT-0554 (FERM BP-6443) cultured in agar slant medium was inoculated into the liquid medium (pH 7.0) containing glucose 2.0%, polypeptone (Nihon Seiyaku Co.) 0.5%, agar 0.1%, yeast extract (Oriental Yeast Co.) 0.2%, magnesium sulfate 7 hydrate 0.05% and pottassium dihydrogen phosphate 0.1% dissolved in 50% natural seawater (salt concentration 3.4% natural seawater was used) and divided into 100 ml in a 500 ml Erlenmeyer flask, and shake cultured at 27° C. for 2 days.
- This seed culture liquid each 1 ml was inoculated into the liquid medium containing potato dextrose broth (Difco Inc.) 2.4% dissolved in 50% natural seawater (salt concentration 3.4% natural seawater was used) divided each 100 ml in a 500 ml Erlenmeyer flask (×30 flasks) and shake cultured at 27° C. for 96 hours.
- Cultured liquid medium was centrifuged and obtained mycelia was extracted with ethanol 3 lit. Ethanol was removed in vacuo. Mycelia was again extracted with n-hexane and concentrated in vacuo to obtain crude substance I, 1.9 g. This was charged on the column of silica gel (95 g, Merck Art. 7734) packed with n-hexane, washed with mixture of n-hexane-ethyl acetate (10:1), and eluated with n-hexane-ethyl acetate (10:2). The eluate was concentrated in vacuo to obtain crude substance II, 22.7 mg. The crude substance II was charged on a silica gel thin layer plates (Merck Art, 5744) and developed with n-hexane-ethyl acetate (1:1). Active fraction was scratched and eluated with chloroform-methanol (2:1). The eluate was concentrated in vacuo to obtain crude substance III, 13.4 mg. Further the substance III was charged on Sephadex LH-20 column and eluated with chloroform-methanol (1:2). The eluate was concentrated in vacuo to obtain FT-0554 substance as white powder 10.0 mg.
- As explained hereinabove, FT-0554 substance of the present invention is expected to be a satisfactory and useful medicament for treatment of parasitosis.
Claims (12)
2. The process according to claim 1 , wherein said microorganism is Aspergillus niger FT-0554.
3. The process according to claim 1 , wherein said microorganism is Aspergillus niger FT-0554 FERM BP-6443.
4. The process according to claim 1 , wherein said microorganism is cultured at a ph of 3-10 and a temperature of 12-45° C.
5. The process according to claim 1 , wherein said microorganism is cultured at a ph of 5-7 and a temperature of 16-36° C.
6. The process according to claim 1 , wherein said medium is a liquid.
7. The process according to claim 1 , wherein said medium contains seawater.
8. The process according to claim 1 , wherein said microorganism is cultured at a ph of 3-10, a temperature of 12-45° C. and in a medium comprising 50-100% seawater.
9. A microorganism belonging to fungi and having the ability to produce FT-0554.
10. The microorganism according to claim 9 , wherein said microorganism is Aspergillus niger FT-0554.
11. The microorganism according to claim 9 , wherein said microorganism is Aspergillus niger FT-0554 FERM BP-6443.
12. A biologically pure culture of Aspergillus niger FT-0554 FERM BP-6443.
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US10/120,523 US20020156126A1 (en) | 1997-11-11 | 2002-04-12 | FT-0554 substance and its production |
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US09/509,770 US6486197B1 (en) | 1997-11-11 | 1998-09-17 | Substance ft-0554 and process for producing the same |
US10/120,523 US20020156126A1 (en) | 1997-11-11 | 2002-04-12 | FT-0554 substance and its production |
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PCT/JP1998/004178 Division WO1999024439A1 (en) | 1997-11-11 | 1998-09-17 | Novel substance ft-0554 and process for producing the same |
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---|---|
US20020156126A1 true US20020156126A1 (en) | 2002-10-24 |
Family
ID=17978403
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09/509,770 Expired - Fee Related US6486197B1 (en) | 1997-11-11 | 1998-09-17 | Substance ft-0554 and process for producing the same |
US10/120,523 Abandoned US20020156126A1 (en) | 1997-11-11 | 2002-04-12 | FT-0554 substance and its production |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09/509,770 Expired - Fee Related US6486197B1 (en) | 1997-11-11 | 1998-09-17 | Substance ft-0554 and process for producing the same |
Country Status (16)
Country | Link |
---|---|
US (2) | US6486197B1 (en) |
EP (1) | EP1033370B1 (en) |
JP (1) | JP4380913B2 (en) |
CN (1) | CN1100781C (en) |
AR (1) | AR018512A1 (en) |
AT (1) | ATE266665T1 (en) |
AU (1) | AU9094998A (en) |
BR (1) | BR9813933A (en) |
DE (1) | DE69823866T2 (en) |
DK (1) | DK1033370T3 (en) |
ES (1) | ES2221194T3 (en) |
HK (1) | HK1026703A1 (en) |
PT (1) | PT1033370E (en) |
TW (1) | TW526201B (en) |
WO (1) | WO1999024439A1 (en) |
ZA (1) | ZA988803B (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2000265986A1 (en) * | 2000-08-22 | 2002-03-04 | The Kitasato Institute | Novel ft-0554a substances and process for the preparation thereof |
AU2002222576A1 (en) * | 2001-12-04 | 2003-06-17 | The Kitasato Institute | Novel substance fki-0550 and process for producing the same |
US7132447B2 (en) * | 2001-12-11 | 2006-11-07 | The Kitasato Institute | Substance FKI-1083 and process for producing the same |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR19990087526A (en) | 1996-03-06 | 1999-12-27 | 하치자에몬 카자마 | New Iridoid Derivatives and Angiogenesis Inhibitors Using the Derivatives as Active Ingredients |
-
1998
- 1998-09-17 BR BR9813933-9A patent/BR9813933A/en not_active Application Discontinuation
- 1998-09-17 WO PCT/JP1998/004178 patent/WO1999024439A1/en active IP Right Grant
- 1998-09-17 DE DE69823866T patent/DE69823866T2/en not_active Expired - Fee Related
- 1998-09-17 AU AU90949/98A patent/AU9094998A/en not_active Abandoned
- 1998-09-17 JP JP2000520448A patent/JP4380913B2/en not_active Expired - Lifetime
- 1998-09-17 ES ES98943018T patent/ES2221194T3/en not_active Expired - Lifetime
- 1998-09-17 US US09/509,770 patent/US6486197B1/en not_active Expired - Fee Related
- 1998-09-17 CN CN98811039A patent/CN1100781C/en not_active Expired - Fee Related
- 1998-09-17 DK DK98943018T patent/DK1033370T3/en active
- 1998-09-17 PT PT98943018T patent/PT1033370E/en unknown
- 1998-09-17 AT AT98943018T patent/ATE266665T1/en not_active IP Right Cessation
- 1998-09-17 EP EP98943018A patent/EP1033370B1/en not_active Expired - Lifetime
- 1998-09-25 ZA ZA988803A patent/ZA988803B/en unknown
- 1998-10-21 AR ARP980105238A patent/AR018512A1/en unknown
- 1998-11-03 TW TW087118287A patent/TW526201B/en not_active IP Right Cessation
-
2000
- 2000-09-20 HK HK00105916A patent/HK1026703A1/en not_active IP Right Cessation
-
2002
- 2002-04-12 US US10/120,523 patent/US20020156126A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
TW526201B (en) | 2003-04-01 |
CN1278824A (en) | 2001-01-03 |
ZA988803B (en) | 1999-04-15 |
ATE266665T1 (en) | 2004-05-15 |
EP1033370B1 (en) | 2004-05-12 |
EP1033370A1 (en) | 2000-09-06 |
PT1033370E (en) | 2004-08-31 |
WO1999024439A1 (en) | 1999-05-20 |
CN1100781C (en) | 2003-02-05 |
ES2221194T3 (en) | 2004-12-16 |
US6486197B1 (en) | 2002-11-26 |
DE69823866D1 (en) | 2004-06-17 |
JP4380913B2 (en) | 2009-12-09 |
HK1026703A1 (en) | 2000-12-22 |
DE69823866T2 (en) | 2005-05-19 |
BR9813933A (en) | 2000-09-19 |
EP1033370A4 (en) | 2001-08-08 |
DK1033370T3 (en) | 2004-09-20 |
AR018512A1 (en) | 2001-11-28 |
AU9094998A (en) | 1999-05-31 |
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Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION |