US20020013281A1 - Derivatives ...use as medicaments - Google Patents
Derivatives ...use as medicaments Download PDFInfo
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- US20020013281A1 US20020013281A1 US09/433,146 US43314699A US2002013281A1 US 20020013281 A1 US20020013281 A1 US 20020013281A1 US 43314699 A US43314699 A US 43314699A US 2002013281 A1 US2002013281 A1 US 2002013281A1
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- 0 *OC1C(OC2[C@@H](C)C(=O)[C@@](*)(C)C(=O)O[C@H](CC)C3(C)OC(=O)N(CCCCn4cnc(-c5ccc(N)cc5)c4)C3[C@@H](C)C(=O)[C@H](C)CC2(C)OC)O[C@H](C)CC1N(C)C Chemical compound *OC1C(OC2[C@@H](C)C(=O)[C@@](*)(C)C(=O)O[C@H](CC)C3(C)OC(=O)N(CCCCn4cnc(-c5ccc(N)cc5)c4)C3[C@@H](C)C(=O)[C@H](C)CC2(C)OC)O[C@H](C)CC1N(C)C 0.000 description 5
- QGACMDJNLPUDIP-UHFFFAOYSA-N NCCCCN1C=NC(C2=CC=C(N)C=C2)=C1 Chemical compound NCCCCN1C=NC(C2=CC=C(N)C=C2)=C1 QGACMDJNLPUDIP-UHFFFAOYSA-N 0.000 description 2
- URLKBWYHVLBVBO-UHFFFAOYSA-N CC1=CC=C(C)C=C1 Chemical compound CC1=CC=C(C)C=C1 URLKBWYHVLBVBO-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D233/00—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings
- C07D233/54—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members
- C07D233/64—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with substituted hydrocarbon radicals attached to ring carbon atoms, e.g. histidine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
- C07H17/08—Hetero rings containing eight or more ring members, e.g. erythromycins
Definitions
- the present invention relates to new derivatives of eryrthromycin, their preparation process and their use as medicaments.
- a subject of the invention is, as new chemical products, the compounds of formula (I)
- X represents a hydrogen atom or a halogen atom
- Z represents a hydrogen atom or the remainder of an acid as well as their addition salts with acids.
- the salts formed with acetic, propionic, trifluoroacetic, maleic, tartaric, methanesulphonic, benzenesulphonic, p-toluenesulphonic acids and particularly stearic, ethylsuccinic or laurylsulphonic acids can be mentioned.
- the halogen atom is for example a chlorine or fluorine atom and preferably a fluorine atom.
- a more particular subject of the invention is the compounds of formula (I) in which Z represents a hydrogen atom.
- a more particular subject of the invention is the compounds, the preparation of which is given hereafter in the experimental part.
- the products of general formula (I) have a very good antibiotic activity on gram ⁇ bacteria such as staphylococci, streptococci, pneumococci.
- the products are particularly active on strains which are resistant to erythromycin such as for example Streptococcus pyogenes and Streptococcus pneumoniae and S. aureus which have an inducible resistance to erythromycin.
- the compounds of the invention can therefore be used as medicaments in the treatment of germ-sensitive infections and in particular, in that of staphylococcia such as staphylococcal septicaemias, malignant staphylococcia of the face or skin, pyodermitis, septic or suppurating wounds, boils, anthrax, phlegmons, erysipelas and acne, staphylococcia such as primitive or post-influenzal acute angina, bronchopneumonia, pulmonary suppuration, streptococcia such as acute angina, otitis, sinusitis, scarlatina, pneumococcia such as pneumonia, bronchitis, and diphtheria.
- staphylococcia such as staphylococcal septicaemias, malignant staphylococcia of the face or skin, pyodermitis, septic or suppurating wounds, boils, anthrax, phleg
- a subject of the invention is, as medicaments, the compounds indicated above as preferred compounds.
- a subject of the invention is also the pharmaceutical compositions containing at least one of the medicaments defined above, as active ingredient.
- compositions can be administered by buccal, rectal, parenteral route, or by local route as a topical application on the skin and mucous membranes, but the preferred administration route is the buccal or injectable route.
- They can be solids or liquids and be presented in the pharmaceutical forms commonly used in human medicine, such as for example, plain or sugar-coated tablets, gelatin capsules, granules, suppositories, injectable preparations, ointments, creams, gels; they are prepared according to the usual methods.
- the active ingredient or ingredients can be incorporated with the excipients usually used in these pharmaceutical compositions such as talc, gum arabic, lactose, starch, magnesium stearate, cocoa butter, aqueous or non-aqueous vehicles, fatty substances of animal or vegetable origin, paraffin derivatives, glycols, various wetting, dispersing or emulsifying agents, preservatives.
- excipients usually used in these pharmaceutical compositions such as talc, gum arabic, lactose, starch, magnesium stearate, cocoa butter, aqueous or non-aqueous vehicles, fatty substances of animal or vegetable origin, paraffin derivatives, glycols, various wetting, dispersing or emulsifying agents, preservatives.
- compositions can also be presented in the form of a powder intended to be dissolved extemporaneously in an appropriate vehicle, for example, apyrogenic sterile water.
- the dose administered is variable according to the affection treated, the patient in question, the administration route and the product considered. It can be, for example, comprised between 50 mg and 3000 mg per day by oral or injectable route for an adult for the preferred products.
- a subject of the invention is also a preparation process characterized in that a compound of formula (II):
- the compound of formula (IA) is subjected to the action of an agent of the hydroxyl function in position 2′ and/or if appropriate, to the action of an acid in order to form the salt.
- reaction of the compound of formula (II) with the compound of formula (III) takes place in a solvent such as for example acetonitrile, dimethylformamide or also tetrahydrofuran, dimethoxy ethane or dimethylsulphoxide,
- a solvent such as for example acetonitrile, dimethylformamide or also tetrahydrofuran, dimethoxy ethane or dimethylsulphoxide
- Compound III is a new product and is itself a subject of the present invention.
- Stage B 2-[4-[4-(4-nitrophenyl)-1H-imidazole-1-yl]butyl]-1H-isoindole-1,3(2H)-dione.
- a solution containing 4.7 g of the product of the preceding stage and 15 ml of DMF is introduced into a mixture containing 1.44 g of sodium hydride and 12.5 ml of DMF.
- a solution containing 7.05 g of N-(4-bromobutyl)phthalimide and 17.5 ml of DMF is added. Agitation is carried out for 3 hours at ambient temperature.
- the reaction medium is poured into a mixture of water and ice, extracted with ethyl acetate, washed with water, dried, filtered and concentrated. 6.77 g of product is obtained which is chromatographed on silica eluting with an ethyl acetate triethylamine mixture 95-5. 2.29 g of product is obtained melting at 170 ⁇ 172° C.
- Stage C 2-[4-[4-(4-aminophenyl)-1H-imidazole-1-yl]butyl]-1H-isoindole-1,3(2H)-dione.
- a mixture of 2 g of product of the preceding stage, 41 ml of a methanol, methylene chloride mixture (20.5 ml-20.5 ml) and 200 mg of 10% palladium on carbon is agitated at ambient temperature under hydrogen pressure for 3 hours.
- the reaction medium is filtered, washed with a methylene chloride methanol mixture 50-50 and concentrated under reduced pressure.
- 1.6 g of product is obtained which is used as it is in the following stage.
- Stage A 11-deoxy 10,11-didehydro 3-de[(2,6-dideoxy 3-0-methyl ⁇ -L-ribohexopyranosyl)oxy]6-0-methyl 3-oxo eryrthromycin.
- Stage B 2′-trimethylsilyloxy of 11-deoxy 10,11-didehydro 3-de [(2,6-dideoxy 3-0-methyl ⁇ -L-ribohexopyranosyl)oxy] 6-0-methyl 3-oxo eryrthromycin.
- a mixture containing 3.08 g of the product of the preceding stage, 340 mg of imidazole, 32 ml of THF anhydre and 1.06 ml of hexamethyl-disilylazane is agitated at ambient temperature for 4 days, followed by evaporating to dryness, taking up in a mixture of 60 ml of methylene chloride and 60 ml of 0.5M sodium acid phosphate.
- the reaction mixture is maintained under agitation for 15 minutes, decanted, extracted with methylene chloride, dried and evaporated to dryness. 3.345 g of sought product is obtained.
- Stage C 2′-trimethylsilyloxy 2 ⁇ -fluoro of 11-deoxy 10,11-didehydro 3-de [(2,6-dideoxy 3-0-methyl ⁇ -L-ribohexo-pyranosyl)oxy]6-0-methyl 3-oxo eryrthromycin.
- Stage D 2 ⁇ -fluoro of 11-deoxy 10,11-didehydro 3-de [(2,6-dideoxy 3-0-methyl 3-0-methyl ⁇ -L-ribohexopyranosyl)oxy] 6-0-methyl 3-oxo eryrthromycin
- a mixture of 5.476 g of the product of the preceding stage, 50 ml of THF and 11.2 ml of 1M tetrabutylammonium fluoride in THF is agitated for 3 hours 30 minutes.
- the solvent is evaporated off and 37 ml of ethyl acetate, 37 ml of water and 7.5 ml of 20% ammonium hydroxide are added. Agitation is carried out for 10 minutes, followed by decanting, extracting with ethyl acetate, drying, filtering and concentrating the filtrate to dryness.
- the product obtained is chromatographed on silica eluting with an ammoniated CH 2 Cl 2 -MeOH mixture 99-1, then 98-2, 97-3, 96-4, 95-5. 2.452 g of sought product is obtained.
- Stage E 2′-acetoxy 2 ⁇ -fluoro of 11-deoxy 10,11-didehydro 3-de [(2,6-dideoxy 3-0-methyl ⁇ -L-ribohexopyranosyl)oxy] 6-0-methyl 3-oxo eryrthromycin
- Stage F 2′-acetoxy 2 ⁇ -fluoro of 12-(oxycarbonyllimidazol) 11-deoxy 10,11-didehydro 3-de [(2,6-dideoxy 3-C-methyl-3-0-methyl ⁇ -L-ribohexopyranosyl)oxy] 6-0-methyl 3-oxo eryrthromycin
- Tablets were prepared containing: Product of Example 1 150 mg Excipient s.q.f. 1 g Detail of excipient: starch, talc, magnesium stearate Product of Example 2 150 mg Excipient s.q.f. 1 g
- a series of tubes were prepared in which the same quantity of nutritive sterile medium is distributed. Increasing quantities of the product to be studied are distributed into each tube, then each tube is sown with a bacterial strain. After incubation for 24 hours in a heating chamber at 37° C., the growth inhibition is evaluated by transillumination, which allows the minimal inhibitory concentrations to be determined, expressed in micrograms/cm 3 . The following results were obtained EX.1 EX.2 Streptococcus pyogenes 2.5 0.3 Streptococcus pneumoniae 1.2 0.150
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Abstract
in which X represents a hydrogen atom or a halogen atom and Z represents a hydrogen atom or the remainder of an acid as well as their addition salts with acids. The compounds of formula (I) have antibiotic properties.
Description
- The present invention relates to new derivatives of eryrthromycin, their preparation process and their use as medicaments.
-
- in which X represents a hydrogen atom or a halogen atom and Z represents a hydrogen atom or the remainder of an acid as well as their addition salts with acids.
- Among the addition salts with acids, the salts formed with acetic, propionic, trifluoroacetic, maleic, tartaric, methanesulphonic, benzenesulphonic, p-toluenesulphonic acids and particularly stearic, ethylsuccinic or laurylsulphonic acids, can be mentioned.
- The halogen atom is for example a chlorine or fluorine atom and preferably a fluorine atom.
- A more particular subject of the invention is the compounds of formula (I) in which Z represents a hydrogen atom.
- A more particular subject of the invention is the compounds, the preparation of which is given hereafter in the experimental part.
- The products of general formula (I) have a very good antibiotic activity on gram⊕ bacteria such as staphylococci, streptococci, pneumococci.
- The products are particularly active on strains which are resistant to erythromycin such as for exampleStreptococcus pyogenes and Streptococcus pneumoniae and S. aureus which have an inducible resistance to erythromycin.
- The compounds of the invention can therefore be used as medicaments in the treatment of germ-sensitive infections and in particular, in that of staphylococcia such as staphylococcal septicaemias, malignant staphylococcia of the face or skin, pyodermitis, septic or suppurating wounds, boils, anthrax, phlegmons, erysipelas and acne, staphylococcia such as primitive or post-influenzal acute angina, bronchopneumonia, pulmonary suppuration, streptococcia such as acute angina, otitis, sinusitis, scarlatina, pneumococcia such as pneumonia, bronchitis, and diphtheria. The products of the present invention are also active against infections caused by germs such as Haemophilus influenzae
- Therefore a subject of the invention is the compounds of formula (I) as medicaments.
- More especially a subject of the invention is, as medicaments, the compounds indicated above as preferred compounds.
- A subject of the invention is also the pharmaceutical compositions containing at least one of the medicaments defined above, as active ingredient.
- These compositions can be administered by buccal, rectal, parenteral route, or by local route as a topical application on the skin and mucous membranes, but the preferred administration route is the buccal or injectable route. They can be solids or liquids and be presented in the pharmaceutical forms commonly used in human medicine, such as for example, plain or sugar-coated tablets, gelatin capsules, granules, suppositories, injectable preparations, ointments, creams, gels; they are prepared according to the usual methods. The active ingredient or ingredients can be incorporated with the excipients usually used in these pharmaceutical compositions such as talc, gum arabic, lactose, starch, magnesium stearate, cocoa butter, aqueous or non-aqueous vehicles, fatty substances of animal or vegetable origin, paraffin derivatives, glycols, various wetting, dispersing or emulsifying agents, preservatives.
- These compositions can also be presented in the form of a powder intended to be dissolved extemporaneously in an appropriate vehicle, for example, apyrogenic sterile water.
- The dose administered is variable according to the affection treated, the patient in question, the administration route and the product considered. It can be, for example, comprised between 50 mg and 3000 mg per day by oral or injectable route for an adult for the preferred products.
-
-
- in order to obtain the corresponding compound of formula (IA) then if desired the compound of formula (IA) is subjected to the action of an agent of the hydroxyl function in position 2′ and/or if appropriate, to the action of an acid in order to form the salt.
- the reaction of the compound of formula (II) with the compound of formula (III) takes place in a solvent such as for example acetonitrile, dimethylformamide or also tetrahydrofuran, dimethoxy ethane or dimethylsulphoxide,
- the hydrolysis of the ester function in position 2′ is carried out using methanol or aqueous hydrochloric acid,
- the salification is carried out using acids according to standard processes.
- The compounds of formula (II) in which X represents a hydrogen atom, which are used as starting products are described and claimed in European Patent Application 0 596 802.
- The compounds of formula (II) which are used as starting products in which X represents a fluorine atom can be prepared as indicated hereafter in the experimental part.
- Compound III is a new product and is itself a subject of the present invention.
- A mixture containing 0.690 g of the product of Preparation 1, 14 ml of THF, 14 ml of isopropanol, 1.41 g of 2′-acetate and 12-[(1H-imidazol-1-yl)carboxylate]of 10,11-didehydro-11-deoxy-3-de [(2,6-dideoxy-3-C-methyl-3-0-methyl-alpha-L-ribohexopyranosyl)oxy]-6-0-methyl-3-oxo-erythromycin and 60 μl of DBU is agitated for 48 hours at ambient temperature. The reaction medium is poured into water, extracted with ethyl acetate, washed with water, dried, filtered and concentrated. 1.54 g of product is obtained which is taken up in 15 ml of methanol with 0.015 ml of DBU added to it. The methanol is driven off under reduced pressure and 1.44 g of product is obtained which is chromatographed on silica eluting with a methylene chloride, methanol, ammonium hydroxide mixture 93-7-0.4. 0.84 g of product is obtained which is taken up in ethyl acetate, water and ammonium hydroxide, followed by extracting, drying, filtering and concentrating. 0.8 g of product is obtained which is chromatographed on silica eluting with an ethyl acetate methanol triethylamine mixture. 0.4 g of product is obtained which is crystallized from ether, separated and dried. 0.270 g of sought product is obtained. M.p.=188-190° C.
- Mass spectrum MH+=826+
NMR CDCl3 ppm Number & ′H Number & ′H 1 1′ 4.28 (d) 2 3.86 q 2′ 3.18 (m) 3 3′ 2.45 (m) 4 3.07 (m) 4′ 1.24-1.68 (m) 5 4.24 (d) 5′ 3.53 (m) 6 5′ Me 1.25 (d) 7 1.60-1.82 (m) N (Me)2 2.26 (s) 8 2.60 masked NCH2 3.64-3.72 (m) 9 CH2 1.65 (m) 10 3.13 (m) CH2 1.85 (m) 11 3.57 (s) CH2N 3.95 (t) 12 N imidazole 7.10-7.45 13 4.94 (dd) 6.70 (d) 7.56 (d) 14 1.54-1.92 (m) NH2 13.53 15 0.84 (t) 2 Me 1.38 (d) 4 Me 1.30 (d) 6 Me 1.34, or, 1.48 (s) 8 Me 1.17 (d) 10 Me 1.00 (d) 12 Me 1.34 or 1.48 (s) 60 Me 2.63 (s) - Stage A: 4-(4-nitrophenyl)-1H-imidazole
- 9.7 g of 2-bromo-1-(4-nitrophenyl)-ethanone and 30 ml of formamide are agitated for 1 hour at 180° C. The reaction medium is cooled down and poured into water. The pH of the reaction medium is adjusted to 1 using a solution of hydrochloric acid and extracted with ethyl acetate. The aqueous phase has concentrated ammonium hydroxide added to it, followed by saturating with sodium chloride and extracting with ethyl acetate. The organic phase is dried, filtered and concentrated under reduced pressure. 7.09 g of product is obtained which is impasted in the ethyl ether, separated and dried. 4.74 g of product is obtained melting at 216 -218° C.
- Stage B: 2-[4-[4-(4-nitrophenyl)-1H-imidazole-1-yl]butyl]-1H-isoindole-1,3(2H)-dione.
- A solution containing 4.7 g of the product of the preceding stage and 15 ml of DMF is introduced into a mixture containing 1.44 g of sodium hydride and 12.5 ml of DMF. A solution containing 7.05 g of N-(4-bromobutyl)phthalimide and 17.5 ml of DMF is added. Agitation is carried out for 3 hours at ambient temperature. The reaction medium is poured into a mixture of water and ice, extracted with ethyl acetate, washed with water, dried, filtered and concentrated. 6.77 g of product is obtained which is chromatographed on silica eluting with an ethyl acetate triethylamine mixture 95-5. 2.29 g of product is obtained melting at 170˜172° C.
- Stage C: 2-[4-[4-(4-aminophenyl)-1H-imidazole-1-yl]butyl]-1H-isoindole-1,3(2H)-dione.
- A mixture of 2 g of product of the preceding stage, 41 ml of a methanol, methylene chloride mixture (20.5 ml-20.5 ml) and 200 mg of 10% palladium on carbon is agitated at ambient temperature under hydrogen pressure for 3 hours. The reaction medium is filtered, washed with a methylene chloride methanol mixture 50-50 and concentrated under reduced pressure. 1.6 g of product is obtained which is used as it is in the following stage.
- Stage D: 4-(4-aminophenyl)-1H imidazole-1-butanamine
- A mixture containing 1.6 g of product of the preceding stage, 1.1 ml of hydrazine hydrate and 35.5 cm3 of absolute ethanol is taken to reflux for 24 hours. The reaction medium is cooled down to ambient temperature, filtered, rinsed with ethanol, concentrated under reduced pressure, taken up in methylene chloride, filtered and concentrated. 0.71 g of sought product is obtained.
- By operating as previously starting with the product of Preparation 2, the sought product is obtained. TLC: ethyl acetate/triethylamine 90-10. Rf 0.20
- Stage A: 11-deoxy 10,11-didehydro 3-de[(2,6-dideoxy 3-0-methyl α-L-ribohexopyranosyl)oxy]6-0-methyl 3-oxo eryrthromycin.
- A mixture of 8.722 g of 2′-acetate of 11-deoxy 10,11-didehydro 3-de[(2,6-dideoxy 3-0-methyl αL-ribohexopyranosyl)oxy]6-0-methyl 3-oxo eryrthromycin (EP 596802) and 350 ml of anhydrous methanol is agitated for 44 hours. 8.794 g of sought product is obtained.
- Stage B: 2′-trimethylsilyloxy of 11-deoxy 10,11-didehydro 3-de [(2,6-dideoxy 3-0-methyl α-L-ribohexopyranosyl)oxy] 6-0-methyl 3-oxo eryrthromycin.
- A mixture containing 3.08 g of the product of the preceding stage, 340 mg of imidazole, 32 ml of THF anhydre and 1.06 ml of hexamethyl-disilylazane is agitated at ambient temperature for 4 days, followed by evaporating to dryness, taking up in a mixture of 60 ml of methylene chloride and 60 ml of 0.5M sodium acid phosphate. The reaction mixture is maintained under agitation for 15 minutes, decanted, extracted with methylene chloride, dried and evaporated to dryness. 3.345 g of sought product is obtained.
- Stage C: 2′-trimethylsilyloxy 2α-fluoro of 11-deoxy 10,11-didehydro 3-de [(2,6-dideoxy 3-0-methyl α-L-ribohexo-pyranosyl)oxy]6-0-methyl 3-oxo eryrthromycin.
- 1.24 ml of a solution of potassium terbutylate in THF 0.97M is added at −12° C. under an argon atmosphere to a solution containing 668 mg of 2′-trimethylsilyloxy of 11-deoxy 10,11-didehydro 3-de [(2,6-dideoxy 3-0-methyl α-L-ribohexopyranosyl)oxy]6-0-methyl 3-oxo eryrthromycin and 6.7 ml of anhydrous THF. The reaction medium is agitated for 5 minutes and 378 mg of N-fluoro dibenzenesulphonimide is added. Agitation is carried out for 10 minutes at −12° C. and the reaction medium is left for 1 hour 30 minutes to return to ambient temperature. Isolation and purification operations are carried out and 695 mg of sought product is obtained.
- Stage D: 2α-fluoro of 11-deoxy 10,11-didehydro 3-de [(2,6-dideoxy 3-0-methyl 3-0-methyl α-L-ribohexopyranosyl)oxy] 6-0-methyl 3-oxo eryrthromycin
- A mixture of 5.476 g of the product of the preceding stage, 50 ml of THF and 11.2 ml of 1M tetrabutylammonium fluoride in THF is agitated for 3 hours 30 minutes. The solvent is evaporated off and 37 ml of ethyl acetate, 37 ml of water and 7.5 ml of 20% ammonium hydroxide are added. Agitation is carried out for 10 minutes, followed by decanting, extracting with ethyl acetate, drying, filtering and concentrating the filtrate to dryness. The product obtained is chromatographed on silica eluting with an ammoniated CH2Cl2-MeOH mixture 99-1, then 98-2, 97-3, 96-4, 95-5. 2.452 g of sought product is obtained.
- Stage E: 2′-acetoxy 2α-fluoro of 11-deoxy 10,11-didehydro 3-de [(2,6-dideoxy 3-0-methyl α-L-ribohexopyranosyl)oxy] 6-0-methyl 3-oxo eryrthromycin
- 1.02 g of the product of Stage D, 10 ml of methylene chloride and 241 μl of acetic anhydride are maintained under agitation for 3 hours. After evaporation is carried out, 10 ml of water and 10 ml of ethyl acetate are added. The reaction medium is left under agitation for 1 hour at ambient temperature, decanted, dried and evaporated. 1.01 g of sought product is obtained.
- Stage F: 2′-acetoxy 2α-fluoro of 12-(oxycarbonyllimidazol) 11-deoxy 10,11-didehydro 3-de [(2,6-dideoxy 3-C-methyl-3-0-methyl α-L-ribohexopyranosyl)oxy] 6-0-methyl 3-oxo eryrthromycin
- 0.388 g of carbonyldiimidazole and 24 μl of DBU is added at 0° C. to a solution containing 1.01 g of the product of the preceding stage and 10 ml of anhydrous THF. The THF is evaporated off and 10 ml of water and 10 ml of ethyl acetate are added. The reaction mixture is maintained under agitation for 10 minutes, extracted, dried and evaporated. 0.902 g of crude sought product is obtained which is chromatographed eluting with an ethyl acetate-triethylamine mixture 96-4. 0.573 g of sought product is obtained.
-
Tablets were prepared containing: Product of Example 1 150 mg Excipient s.q.f. 1 g Detail of excipient: starch, talc, magnesium stearate Product of Example 2 150 mg Excipient s.q.f. 1 g - Detail of excipient: starch, talc, magnesium stearate Injectable solutions were also prepared from salified compounds.
- A—Method of dilutions in liquid medium
- A series of tubes were prepared in which the same quantity of nutritive sterile medium is distributed. Increasing quantities of the product to be studied are distributed into each tube, then each tube is sown with a bacterial strain. After incubation for 24 hours in a heating chamber at 37° C., the growth inhibition is evaluated by transillumination, which allows the minimal inhibitory concentrations to be determined, expressed in micrograms/cm3. The following results were obtained
EX.1 EX.2 Streptococcus pyogenes 2.5 0.3 Streptococcus pneumoniae 1.2 0.150
Claims (9)
2. The compounds of formula (I) in which X represents a fluorine atom.
3. The compounds of formula (I) defined in claim 1 or 2 in which Z represents a hydrogen atom.
4. As a new chemical product defined in claim 1 , the following compound:
11,12-dideoxy-3-de [(2,6-dideoxy-3-C-methyl-3-O-methyl-alpha-L-ribohexopyranosyl)oxy] 6-0-methyl 3-oxo 12,11-[oxycarbonyl[[4-[4-(4-aminophenyl)-1H-imidazol-1-yl]-butyl]imino]]-erythromycin.
5. As a new chemical product defined in claim 1 , the following compound:
2-fluoro-11,12-dideoxy-3-de [(2,6-dideoxy-3-C-methyl-3-O-methyl-alpha-L-ribohexopyranosyl)oxy] 6-0-methyl 3-oxo 12,11-[oxycarbonyl[[4-[4-(4-aminophenyl)-1H-imidazol-1-yl]-butyl]imino]]-erythromycin.
6. Preparation process for the compounds of formula (I) defined in any one of claims 1 to 5 characterized in that a compound of formula (II).
in which X retains its previous meaning and OM represents the remainder of an acyl radical is subjected to the action of a compound of formula (III).
in order to obtain the corresponding compound of formula (IA) then if desired the compound of formula (IA) is subjected to the action of an agent of the hydroxyl function in position 2′ and/or if appropriate, to the action of an acid in order form the salt.
7. As a new chemical product, the compound of formula (III) defined in claim 5 .
8. As a medicament, the compounds of formula (I) defined in any one of claims 1 to 5 as well as their addition salts with pharmaceutically acceptable acids.
9. The pharmaceutical compositions containing at least one medicament according to claim 8 as active ingredient.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/734,162 US6407257B1 (en) | 1998-11-10 | 2000-12-11 | Derivatives of erythromycin, their preparation process and their use as medicaments |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9814145 | 1998-11-10 | ||
FR9814145A FR2785612A1 (en) | 1998-11-10 | 1998-11-10 | NOVEL DERIVATIVES OF ERYTHROMYCIN, PROCESS FOR PREPARING THEM AND THEIR APPLICATION AS MEDICAMENTS |
FR98-14145 | 1998-11-10 |
Related Child Applications (1)
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US09/734,162 Division US6407257B1 (en) | 1998-11-10 | 2000-12-11 | Derivatives of erythromycin, their preparation process and their use as medicaments |
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US20020013281A1 true US20020013281A1 (en) | 2002-01-31 |
US6455505B2 US6455505B2 (en) | 2002-09-24 |
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Application Number | Title | Priority Date | Filing Date |
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US09/433,146 Expired - Lifetime US6455505B2 (en) | 1998-11-01 | 1999-11-03 | Derivatives . . . use as medicaments |
US09/734,162 Expired - Lifetime US6407257B1 (en) | 1998-11-10 | 2000-12-11 | Derivatives of erythromycin, their preparation process and their use as medicaments |
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US09/734,162 Expired - Lifetime US6407257B1 (en) | 1998-11-10 | 2000-12-11 | Derivatives of erythromycin, their preparation process and their use as medicaments |
Country Status (28)
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US (2) | US6455505B2 (en) |
EP (1) | EP1016669B1 (en) |
JP (1) | JP4698783B2 (en) |
KR (1) | KR100567695B1 (en) |
CN (2) | CN1240708C (en) |
AR (1) | AR025814A1 (en) |
AT (1) | ATE232879T1 (en) |
AU (1) | AU766138B2 (en) |
BR (1) | BR9915236A (en) |
CA (1) | CA2350651C (en) |
CZ (1) | CZ20011622A3 (en) |
DE (1) | DE69905447T2 (en) |
DK (1) | DK1016669T3 (en) |
EA (1) | EA200100533A1 (en) |
ES (1) | ES2189365T3 (en) |
FR (1) | FR2785612A1 (en) |
HR (1) | HRP20010335B1 (en) |
HU (1) | HUP0104035A3 (en) |
IL (2) | IL142996A0 (en) |
NO (1) | NO20012272L (en) |
NZ (1) | NZ511609A (en) |
PL (1) | PL348098A1 (en) |
PT (1) | PT1016669E (en) |
SI (1) | SI20640A (en) |
TR (1) | TR200101286T2 (en) |
TW (1) | TWI237027B (en) |
WO (1) | WO2000027857A2 (en) |
ZA (1) | ZA200103801B (en) |
Cited By (5)
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US6777543B2 (en) | 1999-05-24 | 2004-08-17 | Pfizer, Inc. | 13-methyl erythromycin derivatives |
US6833444B2 (en) | 1999-01-27 | 2004-12-21 | Pfizer, Inc. | Ketolide antibiotics |
EP2988597A4 (en) * | 2013-04-04 | 2017-03-08 | President and Fellows of Harvard College | Macrolides and methods of their preparation and use |
US10633407B2 (en) | 2014-10-08 | 2020-04-28 | President And Fellows Of Harvard College | 14-membered ketolides and methods of their preparation and use |
US10640528B2 (en) | 2015-03-25 | 2020-05-05 | President And Fellows Of Havard College | Macrolides with modified desosamine sugars and uses thereof |
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ATE271062T1 (en) | 2000-06-30 | 2004-07-15 | Pfizer Prod Inc | MACROLIDE ANTIBIOTICS |
JP2004017020A (en) * | 2002-06-20 | 2004-01-22 | Sony Corp | Coating method and coated particle |
US7601695B2 (en) | 2003-03-10 | 2009-10-13 | Optimer Pharmaceuticals, Inc. | Antibacterial agents |
EP1794171A2 (en) * | 2004-07-28 | 2007-06-13 | Ranbaxy Laboratories, Ltd. | Ketolide derivatives as antibacterial agents |
WO2006046112A2 (en) * | 2004-10-25 | 2006-05-04 | Ranbaxy Laboratories Limited | Ketolide derivatives as antibacterial agents |
AU2008316830B2 (en) * | 2007-10-25 | 2016-03-17 | Cempra Pharmaceuticals, Inc. | Process for the preparation of macrolide antibacterial agents |
DK2358379T3 (en) * | 2008-10-24 | 2016-03-07 | Cempra Pharmaceuticals Inc | BIOFORSVAR USING TRIAZOLHOLDIGE macrolides |
US9937194B1 (en) | 2009-06-12 | 2018-04-10 | Cempra Pharmaceuticals, Inc. | Compounds and methods for treating inflammatory diseases |
AU2010292010B2 (en) | 2009-09-10 | 2016-01-07 | Cempra Pharmaceuticals, Inc. | Methods for treating malaria, tuberculosis and MAC diseases |
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DK2571506T3 (en) | 2010-05-20 | 2017-08-28 | Cempra Pharmaceuticals Inc | PROCEDURES FOR THE MANUFACTURE OF MACROLIDES AND KETOLIDES AND INTERMEDIATES |
EP2613630A4 (en) | 2010-09-10 | 2014-01-15 | Cempra Pharmaceuticals Inc | Hydrogen bond forming fluoro ketolides for treating diseases |
WO2013148891A1 (en) | 2012-03-27 | 2013-10-03 | Cempra Pharmaceuticals, Inc. | Parenteral formulations for administering macrolide antibiotics |
US9861616B2 (en) | 2013-03-14 | 2018-01-09 | Cempra Pharmaceuticals, Inc. | Methods for treating respiratory diseases and formulations therefor |
CN105188712A (en) | 2013-03-15 | 2015-12-23 | 森普拉制药公司 | Convergent processes for preparing macrolide antibacterial agents |
EP3190122A1 (en) * | 2016-01-08 | 2017-07-12 | LEK Pharmaceuticals d.d. | A novel synthetic pathway towards solithromycin and purification thereof |
CN106432383A (en) * | 2016-09-14 | 2017-02-22 | 重庆两江药物研发中心有限公司 | Solithromycin and preparation method of intermediate thereof |
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JPS5671074A (en) * | 1979-11-12 | 1981-06-13 | Takeda Chem Ind Ltd | 1,2-disubstituted-4-halogenoimidazole-5-acetic acid derivative |
IL99995A (en) * | 1990-11-21 | 1997-11-20 | Roussel Uclaf | Erythromycin derivatives, their preparation and pharmaceutical compositions containing them |
FR2669337B1 (en) * | 1990-11-21 | 1995-03-24 | Roussel Uclaf | NOVEL DESCLADINOSYL DERIVATIVES OF ERYTHROMYCIN, THEIR PREPARATION PROCESS AND THEIR APPLICATION AS MEDICAMENTS. |
FR2697524B1 (en) * | 1992-11-05 | 1994-12-23 | Roussel Uclaf | New erythromycin derivatives, their preparation process and their use as drugs. |
US5527780A (en) * | 1992-11-05 | 1996-06-18 | Roussel Uclaf | Erythromycin derivatives |
FR2719587B1 (en) * | 1994-05-03 | 1996-07-12 | Roussel Uclaf | New erythromycin derivatives, their preparation process and their use as drugs. |
FR2727969B1 (en) * | 1994-12-09 | 1997-01-17 | Roussel Uclaf | NOVEL ERYTHROMYCIN DERIVATIVES, THEIR PREPARATION PROCESS AND THEIR APPLICATION AS MEDICAMENTS |
FR2742757B1 (en) * | 1995-12-22 | 1998-01-30 | Roussel Uclaf | NOVEL ERYTHROMYCIN DERIVATIVES, THEIR PREPARATION PROCESS AND THEIR APPLICATION AS MEDICAMENTS |
ATE296832T1 (en) * | 1996-09-04 | 2005-06-15 | Abbott Lab | 6-O-SUBSTITUTED KETOLIDES WITH ANTIBACTERIAL ACTION |
AU4472897A (en) * | 1997-03-10 | 1998-09-29 | Taisho Pharmaceutical Co., Ltd. | Erythromycin a derivatives |
-
1998
- 1998-11-10 FR FR9814145A patent/FR2785612A1/en active Pending
-
1999
- 1999-11-03 US US09/433,146 patent/US6455505B2/en not_active Expired - Lifetime
- 1999-11-08 TW TW088119491A patent/TWI237027B/en not_active IP Right Cessation
- 1999-11-09 AT AT99402783T patent/ATE232879T1/en active
- 1999-11-09 ES ES99402783T patent/ES2189365T3/en not_active Expired - Lifetime
- 1999-11-09 HU HU0104035A patent/HUP0104035A3/en unknown
- 1999-11-09 CZ CZ20011622A patent/CZ20011622A3/en unknown
- 1999-11-09 IL IL14299699A patent/IL142996A0/en active IP Right Grant
- 1999-11-09 CN CNB998154393A patent/CN1240708C/en not_active Expired - Lifetime
- 1999-11-09 CN CNB200510128838XA patent/CN100351238C/en not_active Expired - Lifetime
- 1999-11-09 DK DK99402783T patent/DK1016669T3/en active
- 1999-11-09 EA EA200100533A patent/EA200100533A1/en unknown
- 1999-11-09 EP EP99402783A patent/EP1016669B1/en not_active Expired - Lifetime
- 1999-11-09 TR TR2001/01286T patent/TR200101286T2/en unknown
- 1999-11-09 SI SI9920091A patent/SI20640A/en not_active IP Right Cessation
- 1999-11-09 AU AU10526/00A patent/AU766138B2/en not_active Expired
- 1999-11-09 DE DE69905447T patent/DE69905447T2/en not_active Expired - Lifetime
- 1999-11-09 PT PT99402783T patent/PT1016669E/en unknown
- 1999-11-09 CA CA2350651A patent/CA2350651C/en not_active Expired - Lifetime
- 1999-11-09 BR BR9915236-3A patent/BR9915236A/en active Search and Examination
- 1999-11-09 WO PCT/FR1999/002718 patent/WO2000027857A2/en active IP Right Grant
- 1999-11-09 KR KR1020017005854A patent/KR100567695B1/en active IP Right Grant
- 1999-11-09 PL PL99348098A patent/PL348098A1/en not_active Application Discontinuation
- 1999-11-09 JP JP31801599A patent/JP4698783B2/en not_active Expired - Lifetime
- 1999-11-09 NZ NZ511609A patent/NZ511609A/en unknown
- 1999-11-10 AR ARP990105702A patent/AR025814A1/en not_active Application Discontinuation
-
2000
- 2000-12-11 US US09/734,162 patent/US6407257B1/en not_active Expired - Lifetime
-
2001
- 2001-05-06 IL IL142996A patent/IL142996A/en not_active IP Right Cessation
- 2001-05-09 NO NO20012272A patent/NO20012272L/en not_active Application Discontinuation
- 2001-05-10 HR HR990077A patent/HRP20010335B1/en not_active IP Right Cessation
- 2001-05-10 ZA ZA200103801A patent/ZA200103801B/en unknown
Cited By (10)
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---|---|---|---|---|
US6833444B2 (en) | 1999-01-27 | 2004-12-21 | Pfizer, Inc. | Ketolide antibiotics |
US6777543B2 (en) | 1999-05-24 | 2004-08-17 | Pfizer, Inc. | 13-methyl erythromycin derivatives |
EP2988597A4 (en) * | 2013-04-04 | 2017-03-08 | President and Fellows of Harvard College | Macrolides and methods of their preparation and use |
US9982005B2 (en) | 2013-04-04 | 2018-05-29 | President And Fellows Of Harvard College | Macrolides and methods of their preparation and use |
US10913764B2 (en) | 2013-04-04 | 2021-02-09 | President And Fellows Of Harvard College | Macrolides and methods of their preparation and use |
US11634449B2 (en) | 2013-04-04 | 2023-04-25 | President And Fellows Of Harvard College | Macrolides and methods of their preparation and use |
US10633407B2 (en) | 2014-10-08 | 2020-04-28 | President And Fellows Of Harvard College | 14-membered ketolides and methods of their preparation and use |
US11466046B2 (en) | 2014-10-08 | 2022-10-11 | President And Fellows Of Harvard College | 14-membered ketolides and methods of their preparation and use |
US10640528B2 (en) | 2015-03-25 | 2020-05-05 | President And Fellows Of Havard College | Macrolides with modified desosamine sugars and uses thereof |
US11535643B2 (en) | 2015-03-25 | 2022-12-27 | President And Fellows Of Harvard College | Macrolides with modified desosamine sugars and uses thereof |
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