US20020012116A1 - Method and apparatus for timing intermittent illumination of a sample tube positioned on a centrifuge platen and for calibrating a sample tube-imaging system - Google Patents
Method and apparatus for timing intermittent illumination of a sample tube positioned on a centrifuge platen and for calibrating a sample tube-imaging system Download PDFInfo
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- US20020012116A1 US20020012116A1 US09/960,468 US96046801A US2002012116A1 US 20020012116 A1 US20020012116 A1 US 20020012116A1 US 96046801 A US96046801 A US 96046801A US 2002012116 A1 US2002012116 A1 US 2002012116A1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/042—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/042—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates
- G01N2015/045—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates by optical analysis
Definitions
- This invention relates to a method for rapidly making centrifuged material layer volume measurements.
- the method of this invention is particularly useful in performing blood constituent count measurements in a centrifuged sample of anticoagulated whole blood.
- the blood sample is centrifuged for about five minutes at about 12,000 RPM, and then the expanded lengths of the cell and platelet layers are measured.
- One of the problems in the patented method pertains to the relatively high centrifuge RPM required to reliably compact the layers, particularly the platelet layer. If the constituent layers are not completely or uniformly packed, the results derived by the method may be inaccurate.
- the aforesaid high RPM centrifugation requires an expensive centrifuge, and increases the risk of tube breakage.
- Another problem relates to the required minimum five minute centrifugation time period, which is undesirable in many medical situations.
- Still another problem relates to the need for the operator to remove the tube from the centrifuge and re-insert it into a reader. Because this operation must be performed within a limited time interval following centrifugation, it requires the close attendance of the operator, which is inefficient and further exposes the operator to a potentially hazardous sample.
- This invention relates to an assembly for quickly determining individual material volume measurements during centrifugation of a gravimetrically separable material mixture sample, such as an anticoagulated whole blood sample. Additionally, this invention relates to a method which can determine blood constituent volume measurements, and blood constituent counts during centrifugation of an anticoagulated whole blood sample before the centrifugation step is finished.
- the method of this invention utilizes a combined centrifuge and reader which will perform the functions of both centrifugation and reading, and thus simplify the performance of the material layer measurements described in the aforesaid U.S. patents.
- the method of this invention provides a kinetic analysis of blood cell compaction.
- white blood cell and platelet layers can be quantified in a relatively low RPM centrifuge, for example a centrifuge which operates at speeds of between about 8,000 to about 10,000 RPM, although the higher speed 12,000 RPM centrifuge can also be used.
- a centrifuge which operates at speeds of between about 8,000 to about 10,000 RPM, although the higher speed 12,000 RPM centrifuge can also be used.
- the fluid component For cell compaction to occur, the fluid component must be displaced, but as the cell layers continue to compact the percolation paths for the fluid become more tortuous. Cell layer compaction will initially progress rapidly but will become increasingly slower as compaction increases and percolation becomes more difficult. Thus the rate of compaction is non-linear. Since the rate of compaction varies considerably between different blood samples due to fluid viscosity and/or other factors, a single reading of a cell layer which is taken prior to complete compaction of the cell layer cannot be extrapolated to predict the extent of the final cell layer compaction. Therefore the thickness (or length) of the fully compacted cell layer cannot be determined from a single measurement of the thickness (or length) of a cell layer which reading is taken during the centrifugation step.
- the method of this invention allows for an accurate calculation of the ultimate extent of blood cell layer compaction, and therefore the thickness of a fully compacted cell layer, over a wide range of centrifuge speeds
- a plurality of cell layer thickness measurements that are taken during the low speed centrifugation of an anticoagulated whole blood sample can accurately predict the ultimate degree of cell layer compaction which will result from a prolonged centrifugation step that is performed at a much higher centrifuge speed, such as is required by the prior art.
- the method of this invention involves the use of: a centrifuge; a fluorescent colorant excitation light source; a photodetector; and a microprocessor controller for controlling operation of the assembly, and for collecting data from readings taken by the assembly.
- the light source is preferentially a high intensity pulsed light source which periodically illuminates the blood sample in the sampling tube as the latter is being centrifuged. Illumination of the blood sample in the tube causes fluorescence of certain of the blood cell constituents as well as illumination of the red blood cell layer, so that the photodetector can discriminate between the various cell layers in the tube that are gravimetrically compacted during the centrifugation step.
- the optics and filters used in performing the method of this invention are generally similar to those described in U.S. Pat. No. 4,558,947, granted Dec. 17, 1985 to S. C. Wardlaw, the disclosure of which patent is incorporated herein in its entirety.
- the light source for Illuminating the tube for the excitation of fluorescence must have sufficient emission in the excitation band (about 420-480 nm) to provide adequate emission energy from the sample tube, when received by a filtered, solid-state image dissector, such as a COD array. Further, the energy must be delivered in the period when the tube to be imaged is within the focal range of the detector, which is typically about 50 ⁇ sec. These requirements are best met by a xenon flash tube having an associated focusing means, rather than a diffuser, which flash tube is driven by a power supply capable of delivering short pulses at the needed power levels and wherein the light flashes are precisely tuned to the position of the tube relative to the detector when the flash tube is triggered.
- the microprocessor controller will be able to calculate the degree of ultimate compaction of the constituent layer or layers being measured, and will display the calculated value. At that point, centrifugation of the sample will be terminated.
- the microprocessor controller controls operation of the assembly in that, on command, it will: initiate centrifugation; monitor the RPM of the centrifuge; synchronize the light pulses with the ongoing centrifuge RPM; control operation of the photodetector; receive and store constituent layer readings; calculate the ultimate degree of constituent layer compaction, and the resultant constituent counts or values; and shut the centrifuge down.
- the operator thus need only place the blood sampling tube in the centrifuge, and initiate operation of the assembly.
- the blood sampling tube can be contained in a special cassette of the general type described in U.S. Pat. No. 5,776,078.
- the object is to measure the final extent of cell compaction following a fixed period of centrifugation
- the layer lengths may be analyzed by the taking of one or more images following the fixed period centrifugation while the centrifuge continues spinning.
- a device for synchronizing the pulsing of the light source irregardless of the speed of rotation of the centrifuge is also provided in the assembly. Such a device takes into account centrifuge wear, eliminates the need to set or adjust the pulsing synchronization of the assembly, and also allows the centrifuge to be intentionally operated at different speeds.
- FIG. 1 is a schematic view of a tube containing a material mixture sample as the latter is being centrifuged in the tube;
- FIG. 2 is a plot of the dynamics of layer compaction during centrifugation of the material mixture, with the layer thickness being plotted against elapsed centrifugation times;
- FIG. 3 is a graph showing how an ultimate platelet layer height was calculated in a specific case when a blood sample was centrifuged at two different speeds, with platelet layer being plotted against the reciprocal of elapsed centrifugation times;
- FIG. 4 is a schematic perspective view of a blood sample measurement assembly used in performing the method of this invention.
- FIG. 5 is a perspective view of a preferred embodiment of a centrifuge platen and drive mechanism designed for use in connection with this invention
- FIG. 6 is a fragmented perspective view of a tube holder and tube rotating mechanisms which are utilized in performing the method of this invention
- FIG. 7 is a sectional view of the tube rotating mechanism used in performing the method of this invention.
- FIG. 8 is a plan view of the centrifuge platen showing the light pulsing synchronizing and imaging system calibration feature of the assembly.
- FIG. 1 there is shown a schematic view of a tube 2 having a transparent side wall 4 and closed at the bottom.
- the tube 2 is filled with a mixture of a particulate material component suspended in a liquid component 6 .
- FIG. 1 illustrates the dynamics of particulate compaction and liquid percolation, as indicated by arrows A and B respectively, both of which occur during centrifugation of the particulate component/liquid component mixture.
- the particulate component will gravimetrically separate from the liquid component 6 , and will, at some point in time, form an interface 8 .
- the interface 8 continually gravitates away from the upper surface 7 of the liquid component 6 as centrifugation continues. It will be understood that more than one interface 8 may form in the sample being centrifuged, depending on the nature of the sample.
- FIG. 2 It should be noted that in a complex mixture of materials, such as whole blood, under some circumstances middle layers may actually increase in thickness rather than compact. This may occur when the separation of a target component from the mixture exceeds the compaction rate of that layer. In all cases, however, the mathematical analysis and extrapolation is performed identically to, and has the same effect as that done on the layers which decrease in thickness.
- the compaction of the cell layers may not immediately follow a hyperbolic function starting at time zero.
- S is the slope of the change
- dP is the change in interface position (or layer thickness)
- t is the elapsed time since the start of centrifugation.
- FIG. 3 shows a specific example of the same anticoagulated whole blood sample centrifuged at two different speeds, wherein the slopes of the hyperbolic curves as shown in FIG. 2, are linearized by plotting the reciprocal of successive elapsed centrifugation times against successive measured thicknesses of the compacted platelet layer interfaces 8 at two different centrifuge speeds, i.e., at 8,000 RPM and 12,000 RPM.
- the line 14 generally denotes the rate of change in the platelet layer thickness during centrifugation of the test samples in a high speed of 12,000 RPM centrifuge; and the line 12 generally denotes the rate of change in the platelet layer thickness during centrifugation of the test samples in a lower speed of 8,000 RPM centrifuge, the latter of which provides only 40% of the G-force of the former.
- centrifuge speeds are considerably different, the end result is the same; and that readings need be taken for only a relatively short period of time, such as for two to three minutes as compared to five to ten minutes, required by the prior art.
- the preliminary measurements may be taken while the centrifuge continues to spin.
- FIG. 4 there is shown a schematic view of a combined centrifuge and reader assembly, which is indicated generally by the numeral 1 .
- the assembly 1 includes a centrifuge platen 3 which includes a recess 5 for holding a transparent capillary tube 9 .
- the tube 9 may be placed directly in the recess 5 , or the capillary tube 9 may be held in a cassette (not shown) of the type disclosed in co-pending patent application U.S. Ser. No. 08/755,363, filed Nov. 25, 1996.
- at least one surface of the tube 9 must be optically visualized for the purpose of collecting the desired optical information from the tube contents.
- the centrifuge platen 3 is rotationally driven by a motor 13 , which is controlled by output line 21 from an assembly microprocessor controller 17 .
- the rotational speed of the motor 13 is monitored by the controller 17 via line 19 thereby allowing the controller 17 to regulate the speed of the motor 13 and thus the centrifuge platen 3 .
- the action of the controller 17 will depend upon the type of analysis required. If it is desired to read the material layer compaction following a fixed period of centrifugation, the controller 17 will energize the motor 13 for a desired fixed period of time and the layer compaction reading will be taken thereafter while the centrifuge continues to spin.
- an indexing device 15 on the side of the platen 3 passes by and interacts with a sensor 23 which sends a signal through line 20 to a programmable delay 22 .
- the indexing device 15 can be a permanent magnet and the sensor 23 can be a Hall-effect sensor.
- the indexing device 15 could be a reflective member on the edge of the platen 3
- the sensor 23 could be an infrared emitter-receiver pair.
- Still another alternative sensing device could include a sensor in the driving motor 13 provided that the platen 3 were rigidly affixed to the shaft of the driving motor 13 .
- a flash driver 24 triggers a flash tube 26 that delivers a brief pulse of light, preferably less than about fifty microseconds in duration.
- a filter and lens assembly 28 focuses the light of the desired wavelength from the flash tube 26 onto the tube 9 .
- the flash tube 26 is positioned beneath the platen 3 , the latter will include an opening 3 ′ between the sample tube 9 and the flash tube 26 and filter and lens assembly 26 .
- the required time delay for timely energizing the flash tube 26 can be determined by the controller 17 and expressed through data bus 30 so as to control operation of the flash driver 24 .
- the tube 9 When the tube 9 is illuminated by the flash tube 26 , the light reflected by the cell layers, or light from fluorescence of the cell layers, is focused by a lens assembly 32 through a light filter set 34 onto a linear image dissector 36 , which is preferably a charge-coupled device (CCD) having at least 256 elements and preferably 5,000 elements so as to achieve optimum optical resolution.
- a linear image dissector 36 which is preferably a charge-coupled device (CCD) having at least 256 elements and preferably 5,000 elements so as to achieve optimum optical resolution.
- Light of an appropriate wave length can be selected by an actuator 38 such as a solenoid or stepping motor and which is controlled by the controller 17 via line 40 whereby the actuator 38 can provide the appropriate filter from the filter set 34 , depending upon the light wavelength selected by the controller 17 .
- electrically variable filters could be used to provide the proper light wavelengths, or CCDs with multiple sensors, each with its own particular filter could be used.
- Suitable electrically variable filters can be obtained from Cambridge Research and Instrumentation, Inc. of Cambridge, Mass.
- Suitable CCD's are available from Sony, Hitachi and others, and are common instrumentation components.
- the electronic shutter in the CCD 36 is opened by the controller 17 , via line 42 .
- the data from the CCD 36 is read into a digitizer 44 which converts the analog signals from each of the CCD cells into a digital signal.
- the digitized data is then transferred to the controller 17 through a data bus 46 so that the data can be immediately analyzed or stored for future examination in the controller 17 .
- optical information from the sample tube 9 can be collected and analyzed by use of the assembly described in co-pending U.S. patent application Ser. No. 08/814,536.
- the mathematical calculations described above which can be used to derive “ultimate compaction” can be made by the controller 17 .
- FIG. 5 there is shown a preferred embodiment of a centrifuge platen assembly 3 which is designed for use when the flashing light source 26 and the digitizer 44 , which are shown in FIG. 4, are positioned above or below the platen 3 .
- the platen 3 is generally dish-shaped and includes an outer rim 50 and a basal floor 52 .
- a central hub 54 is secured to the platen floor 52 , and the hub 54 is closed by a cover 56 .
- the hub 54 has a pair of diametrically opposed windows 58 formed therein.
- the sample tube 9 is mounted on the platen 3 .
- One end of the tube 9 is inserted into an opening in the hub 54 , and the other end of the tube 9 is lowered into a slot 60 formed in a block 62 that is mounted on the platen rim 50 .
- the platen floor 52 is provided with an opening (not shown) covered by transparent plate 64 .
- a counterweight 66 is diametrically disposed opposite the plate 64 so as to dynamically balance the platen 3 .
- the plate 64 allows the platen 3 to be used with light sources and detectors which are located either above or below the platen floor 52 . They also allow the assembly 1 to utilize either reflected light, fluorescent emission or transmitted light in connection with the sample to achieve the desired results.
- the drive shaft of the earlier described centrifuge motor 13 is designated by the numeral 13 ′.
- FIGS. 6 and 7 provide details of the manner in which the motor drive shaft 13 ′ is connected to the platen 3 ; and also details of the manner in which the sample tube 9 is connected to the platen hub 54 .
- the motor drive shaft 13 ′ is affixed to a drive disc 68 which is disposed inside of the hub 54 .
- the disc 68 has a pair of drive pins 70 secured thereto, which drive pins 70 project through the hub windows 58 .
- the drive pins 70 provide the sole driving contact between the motor drive shaft 13 ′ and the platen 3 . Rotation of the disc 68 by the motor drive shaft 13 ′ causes the pins 70 to engage the sides of the hub windows 58 thereby causing the hub 54 and the platen 3 to rotate with the disc 68 .
- a rod 72 is rotatably mounted in the hub 54 .
- the rod 72 includes a collar 74 at an end thereof which collar 74 receives one end of the sample tube 9 .
- the collar 74 houses a resilient O-ring (not shown) which grips the end of the tube 9 .
- a toothed ratchet 76 is mounted on the rod 72 and is operable to cause stepwise selective rotation of the collar 74 and sample tube 9 in the following manner.
- a spring-biased ratchet-engaging pawl 78 is mounted on the disc 68
- a ratchet-engaging blade spring 80 is mounted on the hub cover 56 .
- the momentum of the platen 3 causes it, and its hub 54 , to momentarily rotate at a faster rate than the disc 68 so as to disengage the pawl 78 from the ratchet 76 and to disengage the drive pins 70 from the platen hub 54 .
- the pawl 78 will disengage from the ratchet tooth, and move to a position wherein it engages the next adjacent ratchet tooth.
- the motor 13 is then re-energized to full speed, causing the drive pins 70 to re-engage the hub 54 and causing the pawl 78 to impart a clockwise rotation step of the ratchet 76 and the sample tube 9 .
- FIG. 8 is a plan view of the centrifuge platen 1 showing the sample tube 9 and a detectable target reference device 25 which is disposed on the platen 1 and angularly offset from the tube 9 by a precisely known angle ⁇ .
- the device 25 is preferably formed from a material such as a fluorescent plastic film which will fluoresce or reflect light in a manner similar to that of the tube 9 .
- the device 25 preferably has a plurality of lines or bands 27 which are perpendicular to the axis of the device 25 .
- the device 25 is preferably formed from a stable fluorescent or reflective fluorescent plastic film material so that the energy emitted from the device 25 can be used to calibrate the optical system of the instrument.
- the comparison of energy level responses can be used to determine whether the sample 9 energy level response is low because of a characteristic of the sample 9 , or because of a system error in the instrument's fluorescence response measurement system. If the latter cause of faulty signal level measurements is detected, then the instrument's signal level measurement system will be recalibrated based on the known signal level measurements received from the reference device 25 .
- the length of the bands 27 in the reference device 25 can be sensed by the imaging system in the instrument, and since the length of the bands 27 can be precisely delineated, they can be used for spatial calibration of the entire imaging system.
- One optical error which might occur, for example, is that the imaging lens and filter system may project an image onto the detector 36 which is non-linear.
- equidistant spatial points at the center of the image may not cover the same detector area as those points at the edge of the image. This phenomenon is encountered in photography wherein it is referred to as a pincushion or “barrel” effect, and it prevents accurate spatial measurements unless it is remedied. If the equidistant bands 27 are mapped by the detector 36 across the entire image length, then any distortion can be detected and compensated for in the final calculations.
- the centrifuge platen 1 is rotated in the direction indicated by the arrow A until it reaches operating speed.
- the pulsing of the flash tube 26 is adjusted by the microprocessor 17 until a clear image of the target device 25 is obtained by the image dissector 36 .
- the speed of the centrifuge is first calculated by using the timing pulses given by the sensor 23 . From this data, an assumed delay time from the sensor pulse to the flash trigger is calculated based upon either previously obtained times or a time which is preprogrammed into the instrument when the instrument is assembled. Then a series of images of the target device 25 is captured at that “starting time” and other starting times which are both shorter and longer. The flash time delay which gives the best response from the device 25 is then chosen and implanted into the system's operating software.
- the time delay between the device 25 and the detector 36 can be calculated, and the latter time delay value is added to the time delay from the timing pulse to the detector 25 , so as to provide a total time delay to be programmed into the delay 22 for pulsing the flash tube 26 so as to precisely illuminate the sample tube 9 .
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Abstract
Description
- This invention relates to a method for rapidly making centrifuged material layer volume measurements. The method of this invention is particularly useful in performing blood constituent count measurements in a centrifuged sample of anticoagulated whole blood.
- The measurement of the blood cell counts in a centrifuged sample of anticoagulated whole blood has been described in the scientific literature, and a method for easily measuring certain blood cell and other constituent layers is described in U.S. Pat. No. 4,027,660, granted Jun. 7, 1977 to Stephen C. Wardlaw et al. In the patented method, a sample of anticoagulated whole blood is centrifuged in a precision capillary tube which contains a plastic float. The float linearly expands some of the cell layers and the platelet layer.
- In performing the patented method, the blood sample is centrifuged for about five minutes at about 12,000 RPM, and then the expanded lengths of the cell and platelet layers are measured. One of the problems in the patented method pertains to the relatively high centrifuge RPM required to reliably compact the layers, particularly the platelet layer. If the constituent layers are not completely or uniformly packed, the results derived by the method may be inaccurate. The aforesaid high RPM centrifugation requires an expensive centrifuge, and increases the risk of tube breakage. Another problem relates to the required minimum five minute centrifugation time period, which is undesirable in many medical situations. Still another problem relates to the need for the operator to remove the tube from the centrifuge and re-insert it into a reader. Because this operation must be performed within a limited time interval following centrifugation, it requires the close attendance of the operator, which is inefficient and further exposes the operator to a potentially hazardous sample.
- It would be desirable to be able to measure the blood constituent layers in a shorter period of time, and with a lower RPM centrifuge, and/or to reduce the amount of sample tube handling.
- This invention relates to an assembly for quickly determining individual material volume measurements during centrifugation of a gravimetrically separable material mixture sample, such as an anticoagulated whole blood sample. Additionally, this invention relates to a method which can determine blood constituent volume measurements, and blood constituent counts during centrifugation of an anticoagulated whole blood sample before the centrifugation step is finished. The method of this invention utilizes a combined centrifuge and reader which will perform the functions of both centrifugation and reading, and thus simplify the performance of the material layer measurements described in the aforesaid U.S. patents. The method of this invention provides a kinetic analysis of blood cell compaction. By following the principles of this invention, white blood cell and platelet layers can be quantified in a relatively low RPM centrifuge, for example a centrifuge which operates at speeds of between about 8,000 to about 10,000 RPM, although the higher speed 12,000 RPM centrifuge can also be used.
- During the process of gravimetrically forming cell layers in a centrifuged anticoagulated whole blood sample there are two counteractive forces at work, i.e., outward compaction of the cells and other formed components in the sample; and inward percolation of the plasma in the sample. As the cells and other formed component layers in the blood sample settle during centrifugation, the layers compact thereby decreasing the length of the layers. At the same time, the fluid (plasma) component of the blood sample percolates through the compacting layers.
- For cell compaction to occur, the fluid component must be displaced, but as the cell layers continue to compact the percolation paths for the fluid become more tortuous. Cell layer compaction will initially progress rapidly but will become increasingly slower as compaction increases and percolation becomes more difficult. Thus the rate of compaction is non-linear. Since the rate of compaction varies considerably between different blood samples due to fluid viscosity and/or other factors, a single reading of a cell layer which is taken prior to complete compaction of the cell layer cannot be extrapolated to predict the extent of the final cell layer compaction. Therefore the thickness (or length) of the fully compacted cell layer cannot be determined from a single measurement of the thickness (or length) of a cell layer which reading is taken during the centrifugation step. This fact has formed the basis of the traditional method of determining the optimum centrifuge speed, and time, so that measurements of the cell layers' thickness will be made only after the cell layers would be expected to show no further compaction. “This complete compaction” centrifugation time is considered deemed to be the minimum time required before measuring any centrifuged anticoagulated whole blood constituent layers.
- I have discovered that the inherent unpredictability of the extent of final blood cell layer compaction can be overcome by making several separate preliminary measurements of the cell layers' thickness during the ongoing centrifugation step, and then fitting the derived data to a non-linear mathematical algorithm that will predict the fully compacted cell layer thickness. This procedure does not require ultimate layer compaction, and in fact, the fully compacted cell layer thickness may be mathematically predicted after only four or five preliminary cell layer thickness measurements. Additionally, the method of this invention allows for an accurate calculation of the ultimate extent of blood cell layer compaction, and therefore the thickness of a fully compacted cell layer, over a wide range of centrifuge speeds Thus a plurality of cell layer thickness measurements that are taken during the low speed centrifugation of an anticoagulated whole blood sample can accurately predict the ultimate degree of cell layer compaction which will result from a prolonged centrifugation step that is performed at a much higher centrifuge speed, such as is required by the prior art.
- The method of this invention involves the use of: a centrifuge; a fluorescent colorant excitation light source; a photodetector; and a microprocessor controller for controlling operation of the assembly, and for collecting data from readings taken by the assembly. The light source is preferentially a high intensity pulsed light source which periodically illuminates the blood sample in the sampling tube as the latter is being centrifuged. Illumination of the blood sample in the tube causes fluorescence of certain of the blood cell constituents as well as illumination of the red blood cell layer, so that the photodetector can discriminate between the various cell layers in the tube that are gravimetrically compacted during the centrifugation step. By selection of the appropriate filters on both the light source and the detector, the light reflected from the material layers can be measured, as well as the fluorescence. The pulsing of the light source is synchronized with the position of the tube during centrifugation, so that the tube will be illuminated as it passes by the photodetector. The optics and filters used in performing the method of this invention are generally similar to those described in U.S. Pat. No. 4,558,947, granted Dec. 17, 1985 to S. C. Wardlaw, the disclosure of which patent is incorporated herein in its entirety.
- The light source for Illuminating the tube for the excitation of fluorescence must have sufficient emission in the excitation band (about 420-480 nm) to provide adequate emission energy from the sample tube, when received by a filtered, solid-state image dissector, such as a COD array. Further, the energy must be delivered in the period when the tube to be imaged is within the focal range of the detector, which is typically about 50 μsec. These requirements are best met by a xenon flash tube having an associated focusing means, rather than a diffuser, which flash tube is driven by a power supply capable of delivering short pulses at the needed power levels and wherein the light flashes are precisely tuned to the position of the tube relative to the detector when the flash tube is triggered.
- If the sample is analyzed by the aforesaid kinetic technique, the extent of compaction of the several blood sample constituent layers is periodically imaged during centrifugation, and the sequential constituent layer images are stored in the microprocessor controller in the assembly. After a sufficient number of images have been obtained and stored, about four or five for example, the microprocessor controller will be able to calculate the degree of ultimate compaction of the constituent layer or layers being measured, and will display the calculated value. At that point, centrifugation of the sample will be terminated. The microprocessor controller controls operation of the assembly in that, on command, it will: initiate centrifugation; monitor the RPM of the centrifuge; synchronize the light pulses with the ongoing centrifuge RPM; control operation of the photodetector; receive and store constituent layer readings; calculate the ultimate degree of constituent layer compaction, and the resultant constituent counts or values; and shut the centrifuge down. The operator thus need only place the blood sampling tube in the centrifuge, and initiate operation of the assembly. For operator convenience and safety, the blood sampling tube can be contained in a special cassette of the general type described in U.S. Pat. No. 5,776,078.
- If, on the other hand, the object is to measure the final extent of cell compaction following a fixed period of centrifugation, the layer lengths may be analyzed by the taking of one or more images following the fixed period centrifugation while the centrifuge continues spinning. Thus the advantage of being able to measure the extent of cell layer compaction without having to transfer the blood sample tube from the centrifuge to a separate reader instrument Is realized. A device for synchronizing the pulsing of the light source irregardless of the speed of rotation of the centrifuge is also provided in the assembly. Such a device takes into account centrifuge wear, eliminates the need to set or adjust the pulsing synchronization of the assembly, and also allows the centrifuge to be intentionally operated at different speeds.
- It is therefore an object of this invention to provide a method for deriving an ultimate material layer thickness measurement in a centrifuged material mixture prior to the actual achievement of the ultimate layer thickness.
- It is another object of this invention to provide a method which will allow the reading of the material layer thicknesses following a fixed period of centrifugation while the sample is still within the centrifuge.
- It is a further object of this invention to provide a method of the character described wherein the material mixture is an anticoagulated sample of whole blood.
- It is an additional object of this invention to provide a method of the character described wherein a plurality of preliminary sequential layer interface locations are sensed and stored during centrifugation of the mixture, with the interface data obtained being used to calculate the ultimate layer thickness.
- It is a further object of this invention to provide a method of the character described wherein the ultimate thickness of a plurality of material layers in the sample being centrifuged can be derived from preliminary layer thickness measurements.
- These and other objects and advantages of the invention will become more readily apparent from the following detailed description of the invention when taken in conjunction with the accompanying drawings, in which:
- FIG. 1 is a schematic view of a tube containing a material mixture sample as the latter is being centrifuged in the tube;
- FIG. 2 is a plot of the dynamics of layer compaction during centrifugation of the material mixture, with the layer thickness being plotted against elapsed centrifugation times;
- FIG. 3 is a graph showing how an ultimate platelet layer height was calculated in a specific case when a blood sample was centrifuged at two different speeds, with platelet layer being plotted against the reciprocal of elapsed centrifugation times;
- FIG. 4 is a schematic perspective view of a blood sample measurement assembly used in performing the method of this invention;
- FIG. 5 is a perspective view of a preferred embodiment of a centrifuge platen and drive mechanism designed for use in connection with this invention;
- FIG. 6 is a fragmented perspective view of a tube holder and tube rotating mechanisms which are utilized in performing the method of this invention;
- FIG. 7 is a sectional view of the tube rotating mechanism used in performing the method of this invention; and
- FIG. 8 is a plan view of the centrifuge platen showing the light pulsing synchronizing and imaging system calibration feature of the assembly.
- Referring now to FIG. 1, there is shown a schematic view of a
tube 2 having atransparent side wall 4 and closed at the bottom. Thetube 2 is filled with a mixture of a particulate material component suspended in aliquid component 6. FIG. 1 illustrates the dynamics of particulate compaction and liquid percolation, as indicated by arrows A and B respectively, both of which occur during centrifugation of the particulate component/liquid component mixture. As centrifugation progresses, the particulate component will gravimetrically separate from theliquid component 6, and will, at some point in time, form aninterface 8. Theinterface 8 continually gravitates away from theupper surface 7 of theliquid component 6 as centrifugation continues. It will be understood that more than oneinterface 8 may form in the sample being centrifuged, depending on the nature of the sample. - I have discovered that when the position of the particulate component/liquid, or component/
component interfaces 8 are monitored over the course of time, the sequential positions of the interfaces define afunction curve 10 of the general type depicted in FIG. 2, wherein the initial change in interface position is relatively rapid, but slows appreciably as particulate component compaction progresses. At some point in time, particulate component compaction ceases, and the particulate component-liquid component separation is complete. In the context of this invention, I refer to this cessation of particulate component compaction as “ultimate” compaction which is represented by the dottedline 11 in FIG. 2. - It will be noted that the same phenomenon occurs when a complex mixture of particulate components suspended in a liquid, such as anticoagulated whole blood, is centrifuged. In this case a plurality of particulate component layers will form gravimetrically, with the
liquid component 6 percolating to the top of thecentrifuge tube 2. Blood is an example of such a complex mixture of particulate components, since the red blood cells are heavier than the granulocytes, lymphocytes, monocytes and platelets, in order of decreasing density, and since all of the cells in a blood sample are heavier than the plasma component of the blood sample. When an anticoagulated sample of whole blood is centrifuged the various cell/cell and cell/plasma interfaces will gravitate through the blood sample in the same general manner as is illustrated by - FIG. 2. It should be noted that in a complex mixture of materials, such as whole blood, under some circumstances middle layers may actually increase in thickness rather than compact. This may occur when the separation of a target component from the mixture exceeds the compaction rate of that layer. In all cases, however, the mathematical analysis and extrapolation is performed identically to, and has the same effect as that done on the layers which decrease in thickness.
- The curve described by the rate of change of the location of any particulate constituent interface, and therefore constituent layer thicknesses in a sample undergoing centrifugation is essentially an hyperbola. This fact can be used to mathematically predict the ultimate extent of particulate constituent layer compaction, and therefore the ultimate layer thicknesses and layer volumes of the particulate constituent layer or layers in the centrifuged sample.
- It should be noted that the compaction of the cell layers may not immediately follow a hyperbolic function starting at time zero. To determine when the movement of the interface has reached the point where it follows a hyperbolic function and the ultimate compaction may therefore be calculated, it is only necessary to periodically monitor the interface position or layer thickness and calculate successive slope measurements as S=dP/(1/t), where: S is the slope of the change; dP is the change in interface position (or layer thickness); and t is the elapsed time since the start of centrifugation. When successive values of S no longer change, subsequent preliminary data points may then be collected and the ultimate layer compaction can be determined.
- FIG. 3 shows a specific example of the same anticoagulated whole blood sample centrifuged at two different speeds, wherein the slopes of the hyperbolic curves as shown in FIG. 2, are linearized by plotting the reciprocal of successive elapsed centrifugation times against successive measured thicknesses of the compacted
platelet layer interfaces 8 at two different centrifuge speeds, i.e., at 8,000 RPM and 12,000 RPM. Theline 14 generally denotes the rate of change in the platelet layer thickness during centrifugation of the test samples in a high speed of 12,000 RPM centrifuge; and theline 12 generally denotes the rate of change in the platelet layer thickness during centrifugation of the test samples in a lower speed of 8,000 RPM centrifuge, the latter of which provides only 40% of the G-force of the former. - In order to determine the rate of change slopes, a series of preliminary successive platelet layer thicknesses16 are measured, and least-squares fits are calculated thereby giving the
best path 18 amongst the preliminary layer thickness data points 16. To calculate the ultimate compacted layer thickness, the regression function is extrapolated to an ultimate spin time, in this case infinity, where the value of the reciprocal of the elapsed centrifuge time is zero. The intercept of the rate of change of layer thickness plot at that point on the Y axis identifies the ultimate compacted layer thickness. It is noted that although the centrifuge speeds are considerably different, the end result is the same; and that readings need be taken for only a relatively short period of time, such as for two to three minutes as compared to five to ten minutes, required by the prior art. The preliminary measurements may be taken while the centrifuge continues to spin. - Referring now to FIG. 4, there is shown a schematic view of a combined centrifuge and reader assembly, which is indicated generally by the
numeral 1. Theassembly 1 includes acentrifuge platen 3 which includes arecess 5 for holding a transparentcapillary tube 9. Thetube 9 may be placed directly in therecess 5, or thecapillary tube 9 may be held in a cassette (not shown) of the type disclosed in co-pending patent application U.S. Ser. No. 08/755,363, filed Nov. 25, 1996. In any event, at least one surface of thetube 9 must be optically visualized for the purpose of collecting the desired optical information from the tube contents. Thecentrifuge platen 3 is rotationally driven by amotor 13, which is controlled byoutput line 21 from anassembly microprocessor controller 17. The rotational speed of themotor 13 is monitored by thecontroller 17 vialine 19 thereby allowing thecontroller 17 to regulate the speed of themotor 13 and thus thecentrifuge platen 3. When thecentrifuge platen 3 reaches its predetermined operating speed, which may be between about 8,000 and 12,000 RPM, the action of thecontroller 17 will depend upon the type of analysis required. If it is desired to read the material layer compaction following a fixed period of centrifugation, thecontroller 17 will energize themotor 13 for a desired fixed period of time and the layer compaction reading will be taken thereafter while the centrifuge continues to spin. - On the other hand, if it is desired to kinetically measure the material layer compaction, multiple sequential readings of the gravitationally compacting blood cell layer heights in the
tube 9 are taken. As theplaten 3 rotates, anindexing device 15 on the side of theplaten 3 passes by and interacts with asensor 23 which sends a signal throughline 20 to aprogrammable delay 22. Theindexing device 15 can be a permanent magnet and thesensor 23 can be a Hall-effect sensor. Alternatively, theindexing device 15 could be a reflective member on the edge of theplaten 3, and thesensor 23 could be an infrared emitter-receiver pair. Still another alternative sensing device could include a sensor in the drivingmotor 13 provided that theplaten 3 were rigidly affixed to the shaft of the drivingmotor 13. - After a predetermined time has elapsed from the receipt by the
controller 17 of a signal from theproximity sensor 23, aflash driver 24 triggers aflash tube 26 that delivers a brief pulse of light, preferably less than about fifty microseconds in duration. A filter andlens assembly 28 focuses the light of the desired wavelength from theflash tube 26 onto thetube 9. When theflash tube 26 is positioned beneath theplaten 3, the latter will include anopening 3′ between thesample tube 9 and theflash tube 26 and filter andlens assembly 26. Due to the fact that the exact rotational rotational speed of theplaten 3 is monitored by thecontroller 17 vialine 19, and since the circumferential distance between the position of theindex 15 and the position of thetube 9 is fixed, the required time delay for timely energizing theflash tube 26 can be determined by thecontroller 17 and expressed throughdata bus 30 so as to control operation of theflash driver 24. - When the
tube 9 is illuminated by theflash tube 26, the light reflected by the cell layers, or light from fluorescence of the cell layers, is focused by alens assembly 32 through a light filter set 34 onto alinear image dissector 36, which is preferably a charge-coupled device (CCD) having at least 256 elements and preferably 5,000 elements so as to achieve optimum optical resolution. Light of an appropriate wave length can be selected by anactuator 38 such as a solenoid or stepping motor and which is controlled by thecontroller 17 vialine 40 whereby theactuator 38 can provide the appropriate filter from the filter set 34, depending upon the light wavelength selected by thecontroller 17. Alternatively, electrically variable filters could be used to provide the proper light wavelengths, or CCDs with multiple sensors, each with its own particular filter could be used. Suitable electrically variable filters can be obtained from Cambridge Research and Instrumentation, Inc. of Cambridge, Mass. Suitable CCD's are available from Sony, Hitachi and others, and are common instrumentation components. - Just prior to receiving the light flash from the
flash tube 26, the electronic shutter in theCCD 36 is opened by thecontroller 17, vialine 42. Immediately following the flash, the data from theCCD 36 is read into adigitizer 44 which converts the analog signals from each of the CCD cells into a digital signal. The digitized data is then transferred to thecontroller 17 through adata bus 46 so that the data can be immediately analyzed or stored for future examination in thecontroller 17. Thus at any desired point during the centrifugation process, optical information from thesample tube 9 can be collected and analyzed by use of the assembly described in co-pending U.S. patent application Ser. No. 08/814,536. The mathematical calculations described above which can be used to derive “ultimate compaction” can be made by thecontroller 17. - Referring now to FIG. 5, there is shown a preferred embodiment of a
centrifuge platen assembly 3 which is designed for use when the flashinglight source 26 and thedigitizer 44, which are shown in FIG. 4, are positioned above or below theplaten 3. Theplaten 3 is generally dish-shaped and includes anouter rim 50 and abasal floor 52. Acentral hub 54 is secured to theplaten floor 52, and thehub 54 is closed by acover 56. Thehub 54 has a pair of diametricallyopposed windows 58 formed therein. Thesample tube 9 is mounted on theplaten 3. One end of thetube 9 is inserted into an opening in thehub 54, and the other end of thetube 9 is lowered into aslot 60 formed in a block 62 that is mounted on theplaten rim 50. Theplaten floor 52 is provided with an opening (not shown) covered bytransparent plate 64. Acounterweight 66 is diametrically disposed opposite theplate 64 so as to dynamically balance theplaten 3. Theplate 64 allows theplaten 3 to be used with light sources and detectors which are located either above or below theplaten floor 52. They also allow theassembly 1 to utilize either reflected light, fluorescent emission or transmitted light in connection with the sample to achieve the desired results. The specific example shown in FIGS. 5 and 6 uses a single tube; however, multiple tubes can also be analyzed by theassembly 1 by mounting a sample tube in a diametrically opposed position on theplaten 3, and by altering the timing from the index to the flash so as to provide readings for each separate tube. The drive shaft of the earlier describedcentrifuge motor 13 is designated by the numeral 13′. - FIGS. 6 and 7 provide details of the manner in which the
motor drive shaft 13′ is connected to theplaten 3; and also details of the manner in which thesample tube 9 is connected to theplaten hub 54. Themotor drive shaft 13′ is affixed to adrive disc 68 which is disposed inside of thehub 54. Thedisc 68 has a pair of drive pins 70 secured thereto, which drive pins 70 project through thehub windows 58. The drive pins 70 provide the sole driving contact between themotor drive shaft 13′ and theplaten 3. Rotation of thedisc 68 by themotor drive shaft 13′ causes thepins 70 to engage the sides of thehub windows 58 thereby causing thehub 54 and theplaten 3 to rotate with thedisc 68. Arod 72 is rotatably mounted in thehub 54. Therod 72 includes acollar 74 at an end thereof whichcollar 74 receives one end of thesample tube 9. Thecollar 74 houses a resilient O-ring (not shown) which grips the end of thetube 9. Atoothed ratchet 76 is mounted on therod 72 and is operable to cause stepwise selective rotation of thecollar 74 andsample tube 9 in the following manner. A spring-biased ratchet-engagingpawl 78 is mounted on thedisc 68, and a ratchet-engagingblade spring 80 is mounted on thehub cover 56. When the centrifugemotor drive shaft 13′ is being powered by themotor 13, rotation of thedisc 68 moves thepawl 78 into engagement with one of the teeth on theratchet 76 and theblade spring 80 moves down into engagement with a diametrically opposed tooth on theratchet 76, as shown in FIG. 7. In order to selectively rotate theratchet 76 andsample tube 9, power to thecentrifuge motor 13 is periodically interrupted so as to momentarily slow rotation of thedrive shaft 13′. The momentum of theplaten 3 causes it, and itshub 54, to momentarily rotate at a faster rate than thedisc 68 so as to disengage thepawl 78 from theratchet 76 and to disengage the drive pins 70 from theplaten hub 54. During this momentary disengagement, thepawl 78 will disengage from the ratchet tooth, and move to a position wherein it engages the next adjacent ratchet tooth. Themotor 13 is then re-energized to full speed, causing the drive pins 70 to re-engage thehub 54 and causing thepawl 78 to impart a clockwise rotation step of theratchet 76 and thesample tube 9. When thepawl 78 thus drives the next adjacent ratchet tooth, rotation of theratchet 76 will cause thespring 80 to engage a diametrically opposed next adjacent tooth on theratchet 76 thus stabilizing theratchet 76 and thesample tube 9 in the new rotational position. Stepwise rotation of thesample tube 9 thus allows theimage dissector 36 to “see” the entire periphery of the sample in thetube 9 as the latter is being centrifuged. Circumferential variations in the position of the descendingsample component interfaces 8 will thus be taken into account by the system. The aforesaid pawl and ratchet tube rotating mechanism is the invention of Michael R. Walters of Becton Dickinson and Company, and is described in this application for the purpose of satisfying the “best mode” requirements of the patent statute. - FIG. 8 is a plan view of the
centrifuge platen 1 showing thesample tube 9 and a detectabletarget reference device 25 which is disposed on theplaten 1 and angularly offset from thetube 9 by a precisely known angle Ø. Thedevice 25 is preferably formed from a material such as a fluorescent plastic film which will fluoresce or reflect light in a manner similar to that of thetube 9. Thedevice 25 preferably has a plurality of lines orbands 27 which are perpendicular to the axis of thedevice 25. Thedevice 25 is preferably formed from a stable fluorescent or reflective fluorescent plastic film material so that the energy emitted from thedevice 25 can be used to calibrate the optical system of the instrument. - It is well known that quantitative measurements of fluorescent energy are difficult, in that the energy measured depends on the intensity of the excitation source, the temperature of the sample, and the responsiveness of the detection system, all of which may vary due to a number of factors. Although it is very difficult to provide any type of long term calibration, it is practical to periodically compare the energy level response from a target such as the
reference device 25, which response is stable over time, to the energy level response from thesample 9, which response may not be stable over time. When the energy level response from thesample 9 appears to the instrument to be low, a comparison ofsuccessive sample 9 energy level responses andreference device 25 energy level responses can be made. The comparison of energy level responses can be used to determine whether thesample 9 energy level response is low because of a characteristic of thesample 9, or because of a system error in the instrument's fluorescence response measurement system. If the latter cause of faulty signal level measurements is detected, then the instrument's signal level measurement system will be recalibrated based on the known signal level measurements received from thereference device 25. - Furthermore, the length of the
bands 27 in thereference device 25 can be sensed by the imaging system in the instrument, and since the length of thebands 27 can be precisely delineated, they can be used for spatial calibration of the entire imaging system. One optical error which might occur, for example, is that the imaging lens and filter system may project an image onto thedetector 36 which is non-linear. Thus, equidistant spatial points at the center of the image may not cover the same detector area as those points at the edge of the image. This phenomenon is encountered in photography wherein it is referred to as a pincushion or “barrel” effect, and it prevents accurate spatial measurements unless it is remedied. If theequidistant bands 27 are mapped by thedetector 36 across the entire image length, then any distortion can be detected and compensated for in the final calculations. - In use, the
centrifuge platen 1 is rotated in the direction indicated by the arrow A until it reaches operating speed. The pulsing of theflash tube 26 is adjusted by themicroprocessor 17 until a clear image of thetarget device 25 is obtained by theimage dissector 36. In practice, the speed of the centrifuge is first calculated by using the timing pulses given by thesensor 23. From this data, an assumed delay time from the sensor pulse to the flash trigger is calculated based upon either previously obtained times or a time which is preprogrammed into the instrument when the instrument is assembled. Then a series of images of thetarget device 25 is captured at that “starting time” and other starting times which are both shorter and longer. The flash time delay which gives the best response from thedevice 25 is then chosen and implanted into the system's operating software. - At this point, inasmuch as both the platen speed and the angle between the
target device 25 and thetube 9 are known, the time delay between thedevice 25 and thedetector 36 can be calculated, and the latter time delay value is added to the time delay from the timing pulse to thedetector 25, so as to provide a total time delay to be programmed into thedelay 22 for pulsing theflash tube 26 so as to precisely illuminate thesample tube 9. - It will be appreciated that the aforesaid system and method of operation will suffice to synchronize the flash tube illumination pulses with the passage of the sample tube past the flash tube at any centrifuge platen rotational speed. The aforesaid procedure will have utility, irregardless of aging of centrifuge components, system or sample operating temperatures, or similar operating condition variations. The fluorescent standard device disposed on the platen also allows the imaging system in the assembly to be calibrated and re-calibrated automatically in the field.
- Since many changes and variations of the disclosed embodiment of the invention may be made without departing from the inventive concept, it is not intended to limit the invention otherwise than as required by the appended claims.
Claims (7)
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US4683579A (en) * | 1985-12-27 | 1987-07-28 | Wardlaw Stephen C | Method and apparatus for measuring blood constituent counts |
DE4116313C2 (en) | 1991-05-15 | 1998-11-19 | Lerche Dietmar Prof Dr | Method and device for measuring the elastomechanics of sediments from suspensions and emulsions |
US5279150A (en) * | 1992-03-13 | 1994-01-18 | Katzer Albert E | Automated miniature centrifuge |
FR2754055B1 (en) * | 1996-09-27 | 1998-12-18 | Jouan | DEVICE FOR DETERMINING THE RESISTANT TORQUE OF A ROTATING EQUIPMENT, SYSTEM FOR MONITORING AN ELECTRIC MOTOR AND SYSTEM FOR REGULATING PARAMETERS OF AN ASSOCIATED CENTRIFUGE |
CA2230222A1 (en) * | 1997-03-10 | 1998-09-10 | Stephen C. Wardlaw | Method and assembly for rapid measurement of cell layers |
US5889584A (en) * | 1997-03-10 | 1999-03-30 | Robert A. Levine | Assembly for rapid measurement of cell layers |
US5888184A (en) * | 1997-03-10 | 1999-03-30 | Robert A. Levine | Method for rapid measurement of cell layers |
US5872860A (en) * | 1997-03-13 | 1999-02-16 | Ortho Diagnostic Systems, Inc. | Calibration cassette for use in calibrating an automated agglutination reaction analyzing system |
US6197523B1 (en) * | 1997-11-24 | 2001-03-06 | Robert A. Levine | Method for the detection, identification, enumeration and confirmation of circulating cancer and/or hematologic progenitor cells in whole blood |
US6002474A (en) * | 1998-03-02 | 1999-12-14 | Becton Dickinson And Company | Method for using blood centrifugation device with movable optical reader |
US6262799B1 (en) * | 1999-06-22 | 2001-07-17 | Robert A. Levine | Method and apparatus for rapid measurement of cell layers |
-
1999
- 1999-06-22 US US09/338,044 patent/US6388740B1/en not_active Expired - Fee Related
-
2000
- 2000-06-21 DE DE60030042T patent/DE60030042T2/en not_active Expired - Fee Related
- 2000-06-21 AT AT00113287T patent/ATE336712T1/en not_active IP Right Cessation
- 2000-06-21 EP EP00113287A patent/EP1063513B1/en not_active Expired - Lifetime
-
2001
- 2001-09-24 US US09/960,468 patent/US6441890B2/en not_active Expired - Fee Related
- 2001-09-24 US US09/962,022 patent/US6384906B2/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2017525982A (en) * | 2014-08-28 | 2017-09-07 | シングル テクノロジーズ アクティエボラーグ | High-throughput biochemical screening |
JP7099823B2 (en) | 2014-08-28 | 2022-07-12 | シングル テクノロジーズ アクティエボラーグ | High-throughput biochemical screening |
Also Published As
Publication number | Publication date |
---|---|
EP1063513A3 (en) | 2002-01-30 |
US6384906B2 (en) | 2002-05-07 |
US6441890B2 (en) | 2002-08-27 |
DE60030042D1 (en) | 2006-09-28 |
EP1063513A2 (en) | 2000-12-27 |
ATE336712T1 (en) | 2006-09-15 |
DE60030042T2 (en) | 2007-03-08 |
US6388740B1 (en) | 2002-05-14 |
US20020012117A1 (en) | 2002-01-31 |
EP1063513B1 (en) | 2006-08-16 |
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