US20010008222A1 - Cyclodextrin polymer separation materials - Google Patents
Cyclodextrin polymer separation materials Download PDFInfo
- Publication number
- US20010008222A1 US20010008222A1 US09/214,216 US21421698A US2001008222A1 US 20010008222 A1 US20010008222 A1 US 20010008222A1 US 21421698 A US21421698 A US 21421698A US 2001008222 A1 US2001008222 A1 US 2001008222A1
- Authority
- US
- United States
- Prior art keywords
- cyclodextrin
- water insoluble
- polymer
- group
- aqueous composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 229920000858 Cyclodextrin Polymers 0.000 title claims abstract description 134
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 title claims abstract description 102
- 229920000642 polymer Polymers 0.000 title claims abstract description 101
- 239000000463 material Substances 0.000 title claims description 29
- 238000000926 separation method Methods 0.000 title description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 70
- 229910001868 water Inorganic materials 0.000 claims abstract description 68
- 150000002894 organic compounds Chemical class 0.000 claims abstract description 41
- 239000000203 mixture Substances 0.000 claims abstract description 38
- 239000000178 monomer Substances 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 17
- 239000007795 chemical reaction product Substances 0.000 claims abstract description 15
- 230000003287 optical effect Effects 0.000 claims abstract description 14
- 239000004971 Cross linker Substances 0.000 claims abstract description 11
- 239000005056 polyisocyanate Substances 0.000 claims abstract description 11
- 229920001228 polyisocyanate Polymers 0.000 claims abstract description 11
- BTJIUGUIPKRLHP-UHFFFAOYSA-N 4-nitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-N 0.000 claims description 24
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical class OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims description 24
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims description 21
- 239000001116 FEMA 4028 Chemical class 0.000 claims description 19
- 229960004853 betadex Drugs 0.000 claims description 19
- 239000007787 solid Substances 0.000 claims description 17
- 229920001450 Alpha-Cyclodextrin Polymers 0.000 claims description 12
- HFHDHCJBZVLPGP-RWMJIURBSA-N alpha-cyclodextrin Chemical group OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO HFHDHCJBZVLPGP-RWMJIURBSA-N 0.000 claims description 12
- 229940043377 alpha-cyclodextrin Drugs 0.000 claims description 10
- 239000010409 thin film Substances 0.000 claims description 9
- GDSRMADSINPKSL-HSEONFRVSA-N gamma-cyclodextrin Chemical class OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO GDSRMADSINPKSL-HSEONFRVSA-N 0.000 claims description 7
- 229940080345 gamma-cyclodextrin Drugs 0.000 claims description 7
- 239000000758 substrate Substances 0.000 claims description 4
- 125000000217 alkyl group Chemical group 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 239000012510 hollow fiber Substances 0.000 claims description 2
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 claims description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 2
- 239000012528 membrane Substances 0.000 claims description 2
- 150000004703 alkoxides Chemical group 0.000 claims 1
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 48
- 239000000243 solution Substances 0.000 description 41
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 36
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 33
- 239000000843 powder Substances 0.000 description 20
- 239000005057 Hexamethylene diisocyanate Substances 0.000 description 14
- RRAMGCGOFNQTLD-UHFFFAOYSA-N hexamethylene diisocyanate Chemical compound O=C=NCCCCCCN=C=O RRAMGCGOFNQTLD-UHFFFAOYSA-N 0.000 description 14
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 12
- 239000000047 product Substances 0.000 description 11
- XSTXAVWGXDQKEL-UHFFFAOYSA-N Trichloroethylene Chemical compound ClC=C(Cl)Cl XSTXAVWGXDQKEL-UHFFFAOYSA-N 0.000 description 10
- 238000005259 measurement Methods 0.000 description 10
- DVKJHBMWWAPEIU-UHFFFAOYSA-N toluene 2,4-diisocyanate Chemical compound CC1=CC=C(N=C=O)C=C1N=C=O DVKJHBMWWAPEIU-UHFFFAOYSA-N 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 239000002861 polymer material Substances 0.000 description 9
- UBOXGVDOUJQMTN-UHFFFAOYSA-N trichloroethylene Natural products ClCC(Cl)Cl UBOXGVDOUJQMTN-UHFFFAOYSA-N 0.000 description 9
- 239000003431 cross linking reagent Substances 0.000 description 8
- 238000002835 absorbance Methods 0.000 description 7
- 239000011368 organic material Substances 0.000 description 7
- 238000003756 stirring Methods 0.000 description 7
- 238000000605 extraction Methods 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 6
- 238000011068 loading method Methods 0.000 description 6
- 239000012299 nitrogen atmosphere Substances 0.000 description 6
- SYDNSSSQVSOXTN-UHFFFAOYSA-N 2-nitro-p-cresol Chemical compound CC1=CC=C(O)C([N+]([O-])=O)=C1 SYDNSSSQVSOXTN-UHFFFAOYSA-N 0.000 description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- WFOIXZNEMLWJNK-UHFFFAOYSA-N 3,4-dimethyl-2-nitrophenol Chemical compound CC1=CC=C(O)C([N+]([O-])=O)=C1C WFOIXZNEMLWJNK-UHFFFAOYSA-N 0.000 description 4
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N benzene Substances C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 4
- 229910052801 chlorine Inorganic materials 0.000 description 4
- 239000000460 chlorine Substances 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 125000005442 diisocyanate group Chemical group 0.000 description 4
- 239000010408 film Substances 0.000 description 4
- 239000002957 persistent organic pollutant Substances 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 description 3
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000002983 circular dichroism Methods 0.000 description 3
- 238000010668 complexation reaction Methods 0.000 description 3
- 239000000356 contaminant Substances 0.000 description 3
- 229940097362 cyclodextrins Drugs 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- -1 propylene, butylene, pentylene, hexylene, heptylene, octylene Chemical group 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- UPMLOUAZCHDJJD-UHFFFAOYSA-N 4,4'-Diphenylmethane Diisocyanate Chemical compound C1=CC(N=C=O)=CC=C1CC1=CC=C(N=C=O)C=C1 UPMLOUAZCHDJJD-UHFFFAOYSA-N 0.000 description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 2
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 2
- KYIMHWNKQXQBDG-UHFFFAOYSA-N N=C=O.N=C=O.CCCCCC Chemical compound N=C=O.N=C=O.CCCCCC KYIMHWNKQXQBDG-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 2
- 229920006362 Teflon® Polymers 0.000 description 2
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 2
- 239000003463 adsorbent Substances 0.000 description 2
- 125000001931 aliphatic group Chemical group 0.000 description 2
- 125000002877 alkyl aryl group Chemical group 0.000 description 2
- 125000002947 alkylene group Chemical group 0.000 description 2
- 150000001491 aromatic compounds Chemical class 0.000 description 2
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 2
- 229910052794 bromium Inorganic materials 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- 150000001991 dicarboxylic acids Chemical class 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000002360 explosive Substances 0.000 description 2
- 229910052736 halogen Inorganic materials 0.000 description 2
- 150000002367 halogens Chemical group 0.000 description 2
- 229930195733 hydrocarbon Natural products 0.000 description 2
- 150000002430 hydrocarbons Chemical class 0.000 description 2
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 2
- 239000012948 isocyanate Substances 0.000 description 2
- 150000002513 isocyanates Chemical class 0.000 description 2
- 125000005647 linker group Chemical group 0.000 description 2
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 description 2
- LQNUZADURLCDLV-UHFFFAOYSA-N nitrobenzene Chemical compound [O-][N+](=O)C1=CC=CC=C1 LQNUZADURLCDLV-UHFFFAOYSA-N 0.000 description 2
- 229920006254 polymer film Polymers 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 229910000104 sodium hydride Inorganic materials 0.000 description 2
- 239000012312 sodium hydride Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000010457 zeolite Substances 0.000 description 2
- UOCLXMDMGBRAIB-UHFFFAOYSA-N 1,1,1-trichloroethane Chemical compound CC(Cl)(Cl)Cl UOCLXMDMGBRAIB-UHFFFAOYSA-N 0.000 description 1
- DXBHBZVCASKNBY-UHFFFAOYSA-N 1,2-Benz(a)anthracene Chemical class C1=CC=C2C3=CC4=CC=CC=C4C=C3C=CC2=C1 DXBHBZVCASKNBY-UHFFFAOYSA-N 0.000 description 1
- VEFLKXRACNJHOV-UHFFFAOYSA-N 1,3-dibromopropane Chemical compound BrCCCBr VEFLKXRACNJHOV-UHFFFAOYSA-N 0.000 description 1
- YHRUOJUYPBUZOS-UHFFFAOYSA-N 1,3-dichloropropane Chemical compound ClCCCCl YHRUOJUYPBUZOS-UHFFFAOYSA-N 0.000 description 1
- AAAXMNYUNVCMCJ-UHFFFAOYSA-N 1,3-diiodopropane Chemical compound ICCCI AAAXMNYUNVCMCJ-UHFFFAOYSA-N 0.000 description 1
- WDCYWAQPCXBPJA-UHFFFAOYSA-N 1,3-dinitrobenzene Chemical compound [O-][N+](=O)C1=CC=CC([N+]([O-])=O)=C1 WDCYWAQPCXBPJA-UHFFFAOYSA-N 0.000 description 1
- SGRHVVLXEBNBDV-UHFFFAOYSA-N 1,6-dibromohexane Chemical compound BrCCCCCCBr SGRHVVLXEBNBDV-UHFFFAOYSA-N 0.000 description 1
- OVISMSJCKCDOPU-UHFFFAOYSA-N 1,6-dichlorohexane Chemical compound ClCCCCCCCl OVISMSJCKCDOPU-UHFFFAOYSA-N 0.000 description 1
- QLIMAARBRDAYGQ-UHFFFAOYSA-N 1,6-diiodohexane Chemical compound ICCCCCCI QLIMAARBRDAYGQ-UHFFFAOYSA-N 0.000 description 1
- FNDNSVAJFHHEGH-UHFFFAOYSA-N 1,6-diisocyanatodecane Chemical compound CCCCC(N=C=O)CCCCCN=C=O FNDNSVAJFHHEGH-UHFFFAOYSA-N 0.000 description 1
- DKEGCUDAFWNSSO-UHFFFAOYSA-N 1,8-dibromooctane Chemical compound BrCCCCCCCCBr DKEGCUDAFWNSSO-UHFFFAOYSA-N 0.000 description 1
- WXYMNDFVLNUAIA-UHFFFAOYSA-N 1,8-dichlorooctane Chemical compound ClCCCCCCCCCl WXYMNDFVLNUAIA-UHFFFAOYSA-N 0.000 description 1
- KZDTZHQLABJVLE-UHFFFAOYSA-N 1,8-diiodooctane Chemical compound ICCCCCCCCI KZDTZHQLABJVLE-UHFFFAOYSA-N 0.000 description 1
- KJCVRFUGPWSIIH-UHFFFAOYSA-N 1-naphthol Chemical compound C1=CC=C2C(O)=CC=CC2=C1 KJCVRFUGPWSIIH-UHFFFAOYSA-N 0.000 description 1
- DYSXLQBUUOPLBB-UHFFFAOYSA-N 2,3-dinitrotoluene Chemical compound CC1=CC=CC([N+]([O-])=O)=C1[N+]([O-])=O DYSXLQBUUOPLBB-UHFFFAOYSA-N 0.000 description 1
- UFBJCMHMOXMLKC-UHFFFAOYSA-N 2,4-dinitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O UFBJCMHMOXMLKC-UHFFFAOYSA-N 0.000 description 1
- IQUPABOKLQSFBK-UHFFFAOYSA-N 2-nitrophenol Chemical compound OC1=CC=CC=C1[N+]([O-])=O IQUPABOKLQSFBK-UHFFFAOYSA-N 0.000 description 1
- VYWYYJYRVSBHJQ-UHFFFAOYSA-N 3,5-dinitrobenzoic acid Chemical compound OC(=O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1 VYWYYJYRVSBHJQ-UHFFFAOYSA-N 0.000 description 1
- JZXYEMZTQVSTJC-UHFFFAOYSA-N 4-isocyanatobenzoyl chloride Chemical compound ClC(=O)C1=CC=C(N=C=O)C=C1 JZXYEMZTQVSTJC-UHFFFAOYSA-N 0.000 description 1
- BTJIUGUIPKRLHP-UHFFFAOYSA-M 4-nitrophenolate Chemical compound [O-]C1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-M 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 125000003535 D-glucopyranosyl group Chemical group [H]OC([H])([H])[C@@]1([H])OC([H])(*)[C@]([H])(O[H])[C@@]([H])(O[H])[C@]1([H])O[H] 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- CYTYCFOTNPOANT-UHFFFAOYSA-N Perchloroethylene Chemical compound ClC(Cl)=C(Cl)Cl CYTYCFOTNPOANT-UHFFFAOYSA-N 0.000 description 1
- UATJOMSPNYCXIX-UHFFFAOYSA-N Trinitrobenzene Chemical compound [O-][N+](=O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1 UATJOMSPNYCXIX-UHFFFAOYSA-N 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- 150000001239 acenaphthenes Chemical class 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001454 anthracenes Chemical class 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000003125 aqueous solvent Substances 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- JDPBLCQVGZLACA-UHFFFAOYSA-N benzo[a]perylene Chemical class C1=CC(C=2C3=CC=CC=C3C=C3C=2C2=CC=C3)=C3C2=CC=CC3=C1 JDPBLCQVGZLACA-UHFFFAOYSA-N 0.000 description 1
- TXVHTIQJNYSSKO-UHFFFAOYSA-N benzo[e]pyrene Chemical class C1=CC=C2C3=CC=CC=C3C3=CC=CC4=CC=C1C2=C34 TXVHTIQJNYSSKO-UHFFFAOYSA-N 0.000 description 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 125000004181 carboxyalkyl group Chemical group 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 230000009918 complex formation Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- GJBRTCPWCKRSTQ-UHFFFAOYSA-N decanedioic acid Chemical compound OC(=O)CCCCCCCCC(O)=O.OC(=O)CCCCCCCCC(O)=O GJBRTCPWCKRSTQ-UHFFFAOYSA-N 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 150000002220 fluorenes Chemical class 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 125000005179 haloacetyl group Chemical group 0.000 description 1
- 125000005843 halogen group Chemical group 0.000 description 1
- VLKZOEOYAKHREP-UHFFFAOYSA-N hexane Substances CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 1
- YVSCCMNRWFOKDU-UHFFFAOYSA-N hexanedioic acid Chemical compound OC(=O)CCCCC(O)=O.OC(=O)CCCCC(O)=O YVSCCMNRWFOKDU-UHFFFAOYSA-N 0.000 description 1
- BVKZIAODKDJPLW-UHFFFAOYSA-N hexanitrobenzene Chemical compound [O-][N+](=O)C1=C([N+]([O-])=O)C([N+]([O-])=O)=C([N+]([O-])=O)C([N+]([O-])=O)=C1[N+]([O-])=O BVKZIAODKDJPLW-UHFFFAOYSA-N 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 125000002768 hydroxyalkyl group Chemical group 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 150000002469 indenes Chemical class 0.000 description 1
- INQOMBQAUSQDDS-UHFFFAOYSA-N iodomethane Chemical compound IC INQOMBQAUSQDDS-UHFFFAOYSA-N 0.000 description 1
- IQPQWNKOIGAROB-UHFFFAOYSA-N isocyanate group Chemical group [N-]=C=O IQPQWNKOIGAROB-UHFFFAOYSA-N 0.000 description 1
- 125000001261 isocyanato group Chemical group *N=C=O 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 150000002790 naphthalenes Chemical class 0.000 description 1
- VLZLOWPYUQHHCG-UHFFFAOYSA-N nitromethylbenzene Chemical compound [O-][N+](=O)CC1=CC=CC=C1 VLZLOWPYUQHHCG-UHFFFAOYSA-N 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 150000002979 perylenes Chemical class 0.000 description 1
- 150000002987 phenanthrenes Chemical class 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920005547 polycyclic aromatic hydrocarbon Polymers 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 150000003220 pyrenes Chemical class 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 229920013730 reactive polymer Polymers 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000013077 target material Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 150000003518 tetracenes Chemical class 0.000 description 1
- 229950011008 tetrachloroethylene Drugs 0.000 description 1
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- RUELTTOHQODFPA-UHFFFAOYSA-N toluene 2,6-diisocyanate Chemical compound CC1=C(N=C=O)C=CC=C1N=C=O RUELTTOHQODFPA-UHFFFAOYSA-N 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G18/00—Polymeric products of isocyanates or isothiocyanates
- C08G18/06—Polymeric products of isocyanates or isothiocyanates with compounds having active hydrogen
- C08G18/28—Polymeric products of isocyanates or isothiocyanates with compounds having active hydrogen characterised by the compounds used containing active hydrogen
- C08G18/40—High-molecular-weight compounds
- C08G18/64—Macromolecular compounds not provided for by groups C08G18/42 - C08G18/63
- C08G18/6484—Polysaccharides and derivatives thereof
Definitions
- the present invention relates to cyclodextrin polymer materials and to the use of such cyclodextrin polymer materials as separation materials for separation or removal of, e.g., organic contaminants from aqueous compositions. More particularly, the present invention relates to water insoluble cyclodextrin polymer materials. This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).
- ⁇ -cyclodextrin polymer films formed by crosslinking of a soluble ⁇ -cyclodextrin polymer (partially crosslinked with 1-chloro-2,3-epoxypropane) with glutaric aldehyde.
- the polymer films were studied in conjunction with a 4-nitrophenol/4-nitrophenolate guest system. Zhao et al., Reactive Polymers, vol. 24, pp. 9-16, 1994, describe ⁇ -cyclodextrin immobilized onto crosslinked styrene/divinylbenzene copolymer to form a ⁇ -cyclodextrin polymeric adsorbent.
- This adsorbent demonstrated apparent inclusion ability for isomeric compounds such as 2- and 4-nitro-substituted aromatic compounds, e.g., 2-nitrophenol, 4-nitrophenol and 2,4-dinitrophenol.
- cyclodextrin derivatives for adsorption or extraction of certain organic materials.
- U.S. Pat. No. 5,190,663 uses cyclodextrin anchored to a water insoluble substrate or carrier particle to remove dissolved polynuclear aromatic hydrocarbons from an aqueous composition.
- U.S. Pat. No. 5,425,881 uses aqueous solutions of cyclodextrins or cyclodextrin derivatives in extraction of an organic pollutant from contaminated soil and also describes water soluble cyclodextrin polymers wherein the cyclodextrin is crosslinked with epichlorohydrin or isocyanate.
- Another object of the invention is to provide water insoluble cyclodextrin polymer materials having a defined nanoporous structure.
- target organic compounds e.g., organic pollutants or contaminants
- the present invention provides a water insoluble polymeric composition
- a water insoluble polymeric composition comprising a reaction product of a cyclodextrin monomer and a polyfunctional crosslinker selected from the group consisting of polyisocyanates, dihalohydrocarbons, and dihaloacetylhydrocarbons.
- the present invention further provides a process for removing a target organic compound from an aqueous composition
- a process for removing a target organic compound from an aqueous composition comprising contacting said aqueous composition containing a target organic compound with a water insoluble cyclodextrin polymer comprising a reaction product of a cyclodextrin monomer and a polyfunctional crosslinker selected from the group consisting of polyisocyanates, dihalohydrocarbons, and dihaloacetylhydrocarbons for time sufficient to form a reaction product between said water insoluble cyclodextrin polymer and said target organic compound whereby the concentration of said target organic compound in said aqueous composition is reduced.
- FIG. 1 is a graph showing induced circular dichroism of a cyclodextrin polymer complex with para-nitrophenol.
- FIG. 2 is a graph showing a second harmonic generation signal versus incident angle.
- FIG. 3 is a graph showing estimation on pore size by plotting actual loading of various organic materials having varying critical or maximum dimensions.
- the present invention is concerned with cyclodextrin polymers and to the use of such cyclodextrin polymers as separation materials for separating selected organic materials from aqueous streams or compositions.
- the cyclodextrin polymers of the present invention are generally formed by the reaction of a suitable cyclodextrin monomer with a polyfunctional crosslinking agent.
- the crosslinking agent may generally be an aromatic, an aliphatic or a cycloaliphatic polyfunctional crosslinking agent.
- Suitable polyfunctional crosslinking agents can include diisocyanates, polyisocyanates, dihalohydrocarbons, and dihaloacetylhydrocarbons.
- the polyfunctional crosslinking agent of the present invention can include asymmetric crosslinking agents containing different linking functionalities from among the functionalities of isocyanate, halo, or haloacetyl, on the linking molecule, e.g., at the ends of the molecule.
- a suitable asymmetric crosslinking agent may be 4-isocyanatobenzoyl chloride and the like.
- the polyfunctional crosslinking agents include at least one isocyanate group or functionality.
- the cyclodextrin polymers of this invention are characterized as water insoluble.
- water insoluble is a relative term and as used herein generally refers to materials having a solubility in water of no greater than about 0.01 grams per gram of water.
- the cyclodextrin polymers of this invention can have a nanoporous structure capable of absorbing selected target organic compounds from within aqueous streams, solutions or compositions down to levels as low as parts per billion (ppb) and even to levels of parts per trillion (ppt).
- Diisocyanates can include such as 2,6-tolylene diisocyanate, 2,4-tolylene diisocyanate (TDI), 4,4′-diphenylmethane diisocyanate (MDI), hexamethylene diisocyanate (HDI), and the like.
- TDI 2,4-tolylene diisocyanate
- MDI 4,4′-diphenylmethane diisocyanate
- HDI hexamethylene diisocyanate
- Dihalohydrocarbons can be generally represented by the formula X-R 1 -X where X is a halogen selected from among chlorine, bromine and iodine, preferably chlorine, and R 1 is an alkylene group such as propylene, butylene, pentylene, hexylene, heptylene, octylene and the like, an alkylaryl group such as dimethylenebenzene, dipropylenebenzene and the like.
- X is a halogen selected from among chlorine, bromine and iodine, preferably chlorine
- R 1 is an alkylene group such as propylene, butylene, pentylene, hexylene, heptylene, octylene and the like, an alkylaryl group such as dimethylenebenzene, dipropylenebenzene and the like.
- suitable dihalohydrocarbons may include 1,3-dichloropropane, 1,3-dibromopropane, 1,3-diiodopropane, 1,6-dichlorohexane, 1,6-dibromohexane, 1,6-diiodohexane, 1,8-dichlorooctane, 1,8-dibromooctane, 1,8-diiodooctane, 1,4-chloromethylenebenzene, 1,4-bromomethylenebenzene, and 1,4-iodomethylenebenzene.
- Dihaloacetylhydrocarbons can be generally represented by the formula XOC-R 2 -COX where X is a halogen selected from among chlorine, bromine and iodine, preferably chlorine, and R 2 is an alkylene group such as propylene, butylene, pentylene, hexylene, heptylene, octylene and the like, an alkylaryl group such as dimethylenebenzene, dipropylenebenzene and the like.
- X is a halogen selected from among chlorine, bromine and iodine, preferably chlorine
- R 2 is an alkylene group such as propylene, butylene, pentylene, hexylene, heptylene, octylene and the like, an alkylaryl group such as dimethylenebenzene, dipropylenebenzene and the like.
- Suitable dihaloacetylhydrocarbons may be generally prepared by chlorination of dibasic acids such as dicarboxylic acids and specific examples of dicarboxylic acids may include 1,4-butanedicarboxylic acid (adipic acid), ortho-benzene dicarboxylic acid (oxalic acid), cis-butenedioic acid (maleic acid), and decanedioic acid (sebacic acid).
- dibasic acids such as dicarboxylic acids and specific examples of dicarboxylic acids may include 1,4-butanedicarboxylic acid (adipic acid), ortho-benzene dicarboxylic acid (oxalic acid), cis-butenedioic acid (maleic acid), and decanedioic acid (sebacic acid).
- Suitable cyclodextrin monomer materials include ⁇ -cyclodextrin, ⁇ -cyclodextrin, ⁇ -cyclodextrin or substituted ⁇ -cyclodextrins, substituted ⁇ - cyclodextrins, or substituted ⁇ -cyclodextrins, preferably substituted ⁇ -cyclodextrins, substituted ⁇ -cyclodextrins, or substituted ⁇ -cyclodextrins.
- cyclodextrins are linked D-glucopyranose units, with ⁇ -cyclodextrin, ⁇ -cyclodextrin, ⁇ -cyclodextrin being composed of 6, 7, or 8 units, respectively, the units linked into a circular arrangement. Accordingly, the internal diameter of each of ⁇ -cyclodextrin, ⁇ -cyclodextrin, ⁇ -cyclodextrin varies from the others.
- ⁇ -cyclodextrin has a cavity size or internal diameter of about 4.7 to 5.2 Angstroms (A)
- ⁇ -cyclodextrin has an internal diameter of about 6.0 to 6.5A
- ⁇ -cyclodextrin has an internal diameter of about 7.5 to 8.5A.
- Branched cyclodextrin monomer materials may also be employed.
- substituted cyclodextrin refers to a cyclodextrin modified by the addition of other functional groups, e.g., a cyclodextrin wherein a hydrogen atom of one or more primary or secondary hydroxyl groups therein has been substituted by, e.g., a carboxyl group, a carboxyl alkyl group, a carboxylaryl group, an alkyl group, e.g., either a lower alkyl such as a C 1 to C 4 group, i.e., methyl, ethyl, propyl or butyl, or a longer chain aliphatic containing from about 8 to about 22 carbons, a hydroxyalkyl group, a sulfonic group, or an alkylenesulfonic group and the like. Modification of a cyclodextrin can alter the length and size of the internal cavity or alter the chemical compatibility or binding properties of the particular substituted
- One manner of preparing a substituted cyclodextrin polymer may be to modify or fuctionalize a cyclodextrin monomer prior to polymerization of the monomer.
- Another manner of preparing a substituted cyclodextrin polymer may be to polymerize a cyclodextrin monomer and then to modify or functionalize the resultant cyclodextrin polymer.
- the substituted cyclodextrin monomer is prepared prior to polymerization of the substituted cyclodextrin monomer.
- One benefit of tailoring the substituted cyclodextrin functionality may be to alter the retention times of the particular target organic species.
- the process of the present invention is characterized by the feature that the initial concentration of the target organic compounds in the aqueous composition is generally relatively low and the final concentration of the target organic compounds after treatment with the cyclodextrin polymers of the present invention is extremely low.
- the cyclodextrin polymers and the substituted cyclodextrin polymers of the present invention have been found to be selective for the target organic compounds and can generally effect essentially complete removal of such target organic compounds contained within a sample of water so long as the concentration of organic compounds is not so great to exceed the amount of cyclodextrin polymer material used.
- an aqueous composition including a relatively low concentration of target organic compounds is contacted with a water insoluble cyclodextrin polymer comprising the reaction product of a cyclodextrin monomer and a polyfunctional crosslinker selected from among polyisocyanates, dihalohydrocarbons, and dihaloacetylhydrocarbons.
- a polyfunctional crosslinker selected from among polyisocyanates, dihalohydrocarbons, and dihaloacetylhydrocarbons.
- Asymmetrical crosslinkers may also be employed.
- the process is carried out under conditions such as at temperatures and for periods of time sufficient to reduce the amount of target organic compounds to a preselected level.
- the entire operation can be carried out at ambient conditions in which case complexation of the target organic compounds is fairly rapid, and contact times between the water insoluble cyclodextrin polymer and the aqueous composition can be as short as about five seconds or less. Increasing the contact time has no detrimental effect on the process and may in fact increase removal efficiency of the target organic compounds. There is a tradeoff between the process conditions and the amount of water insoluble cyclodextrin polymer used. There is a general stoichiometric reaction between cyclodextrins and target organic compounds. The reaction is first order, i.e., the rate correlates with concentration and surface area. Increasing the surface area or organic concentration will increase reaction rate. Increasing temperature will also increase the reaction rate of removal of organics. Thus, if complexation is inhibited by rapid contact rates, high temperatures or when purifying an aqueous stream containing high hydrocarbon levels, increasing the amount of water insoluble cyclodextrin polymer generally increases removal efficiency of the target organic compounds.
- the process of the present invention can be conducted by using a powder of the water insoluble cyclodextrin polymer where such powder is supported in a packed column, cartridge, or bed through which the aqueous composition is passed at a suitable rate to effect removal of the target organic compounds.
- a larger piece (as opposed to a powder) of the water insoluble cyclodextrin polymer e.g., a piece having dimensions of at least about one quarter inch by about one quarter inch by about one quarter inch, or a spherical piece having a diameter of at least about one quarter inch, can be placed in contact with a quantity of water containing a undesirable amount of the target organic compounds.
- the specific size of the larger piece is not critical and smaller pieces, greater than a typical powder, can be used singly or in combination with other small pieces to extract target organic compounds from a water sample such as a well and the like.
- such an undesirable amount of the target organic compounds is an amount exceeding some defined level such as levels set by agencies such as the United States Environmental Protection Agency (EPA).
- EPA United States Environmental Protection Agency
- the concentration of the target organic compounds will normally be reduced to a level which is prescribed for such pollutants or to a level lower than present conventional detection limits.
- a thin film of the water insoluble cyclodextrin polymer can be formed on a support substrate such as a glass substrate, or on beads, and the supported thin film of the water insoluble cyclodextrin polymer contacted with the aqueous stream including the target organic compounds.
- a thin film can typically be of a thickness from about 0.01 microns to about 5 millimeters. Hollow fibers of the cyclodextrin polymer may also be employed.
- the target organic compounds can be separated from the water insoluble cyclodextrin polymer by extraction with a suitable extraction agent or solvent.
- suitable extraction agents or solvents can include alcohols such as methanol, ethanol and the like.
- aromatic compounds e.g., benzene, toluene, xylene and the like
- polyaromatic compounds including compounds with fused ring structures containing between about two and ten rings, some or all of which are benzene rings, e.g., naphthalenes, indenes, anthracenes, phenanthrenes, fluorenes, acenaphthenes, benzanthracenes, perylenes, tetracenes, pyrenes, benzopyrenes, benzoperylenes, and the like, oxygen-containing organic compounds, e.g., methanol, acetone, dimethyl sulfoxide, dimethyl formamide, tetrahydrofuran and the like, halogenated, e.g., brominated or chlorinated, hydrocarbons, e.g., chloroform
- the cyclodextrin polymer can lower the concentration of some organic materials to as low as about 3 parts per trillion (ppt), far lower than a conventional separation material of activated carbon at about 1.3 parts per million (ppm).
- Activated carbon is often used in typical pump and treat systems for groundwater contamination. While activated carbon has a higher loading capacity at 58 milligrams per gram than the cyclodextrin polymer, the activated carbon can be leached by additional water to contaminate further water whereas the cyclodextrin polymer will bind the target organic until eluation with some non-aqueous solvent such as ethanol.
- the reaction product (often referred to as a complexation product) of certain organic compounds with the water insoluble cyclodextrin polymer can have nonlinear optical properties such that the reaction product can be characterized as an organic nonlinear optical material.
- Optical quality thin films can be prepared from the water insoluble cyclodextrin polymers of the present invention. Such thin films can then absorb organic chromophores from water. Some chromophores can be nonlinear optical materials, typically for polar molecules.
- chromophores may be included 4-nitrophenol, 4-nitrostyryl-4′-phenol, 4-hydroxylstilbazole, and 4-hydroxylstilbazolium iodide.
- Such polar molecules will have a preferential orientation inside the cavity of a cyclodextrin material since the polar nature of the water-polymer interface will generally cause the chromophore to orient prior to entering the polymeric matrix. Then, once the chromophore enters the solid cyclodextrin polymer material, the chromophores retain the alignment and can possess second order nonlinear optical properties.
- Organic nonlinear optical materials offer potential for use in integrated optical devices.
- TDI toluene 2,4-diisocyanate
- TDI toluene 1,6-diisocyanate
- the reactivity of the bi-functional linkers i.e., the HDI, TDI and DDI was observed to be: TDI>HDI>DDI.
- the hydrophobicity of the resulting cyclodextrin polymers varied with the bi-functional linker with DDI>HDI>TDI.
- infrared measurements indicated that the isocyanato groups had disappeared and peaks corresponding to O—C ⁇ O, O ⁇ C—NH and NH groups were observed.
- a water solution, total volume 4.16 liters, containing about 3 ⁇ 10 ⁇ 9 moles per liter (M) of para-nitrophenol was passed through a glass column packed with 0.5858 g of powder of the cyclodextrin polymer from example 1.
- the powder gradually turned visibly yellow in color from its initial clear, colorless appearance. Retention of para-nitrophenol by the cyclodextrin polymer powder was confirmed.
- the final solution concentration of para-nitrophenol was measured as 1.44 ⁇ 10 ⁇ 10 M.
- the para-nitrophenol was then separated from the cyclodextrin polymer powder by washing of the cyclodextrin polymer powder with ethanol.
- the non-covalent binding of the para-nitrophenol to the cyclodextrin polymer powder allowed the separation of the para-nitrophenol from the cyclodextrin polymer powder by washing with an organic solvent such as ethanol.
- Formation constants were calculated as 6.93 ⁇ 10 ⁇ 9 M ⁇ 1 for the HDI- ⁇ -CD/para-nitrophenol complex and as 1.64 ⁇ 10 9 M ⁇ 1 for the TDI- ⁇ -CD/ para-nitrophenol complex.
- FIG. 1 A sample of the resultant product between the HDI- ⁇ -CD and the para-nitrophenol was measured and contrasted with a sample of the HDI- ⁇ -CD. Measurements for induced circular dichroism are shown in FIG. 1 where solid line 10 shows the plot for the resultant product between the HDI- ⁇ -CD and the para-nitrophenol while dashed line 12 shows the plot for the sample of HDI- ⁇ -CD. The peak in line 10 at about 400 nanometers (nm) indicates the induced circular dichroism due to complex formation.
- a bulk portion of the polymer from example 1 was immersed in a one liter water solution containing about 2 ⁇ 10 ⁇ 7 M of para-nitrophenol for one day.
- the solid polymer (about 0.5 g) became visibly yellow after which it was removed from the solution.
- the final solution concentration of para-nitrophenol was measured as 1.8 ⁇ 10 ⁇ 10 M.
- the solid was then washed with ethanol whereupon para-nitrophenol was removed from the solid polymer until it again appeared clear and colorless.
- the methyl-substituted cyclodextrin was then polymeric with HDI as follows.
- a measurement of the binding constant of the polymer from example 9 with toluene was conducted as follows.
- Binding or equilibrium constant (K) measurements were as follows.
- the polymers (0.8292 g for the polymer of example 1 and 0.8751 gram for the polymer of example 9) were each immersed in 1000 ml of the aqueous toluene solution (4.6939 ⁇ 10 ⁇ 7 M) and stirred for 1 day.
- the final toluene concentration in the water was 8.85 ⁇ 10 ⁇ 9 M for the polymer of example 1 and 3.76 ⁇ 10 ⁇ 9 M for the polymer of example 9, respectively.
- the polymer was filtered off and washed with ethanol.
- the volume of ethanol was concentrated to around 5 ml.
- TCE trichloroethylene
- Binding or equilibrium constant (K) measurements were as follows. The polymers (0.8818 g for example 1 polymer; 0.8008 gram for example 9 polymer) were immersed in 2000 ml of the aqueous TCE solution (7.589 ⁇ 10 ⁇ 8 M) and stirred for 1 day. The final TCE concentration in the water was found to be 1.05 ⁇ 10 ⁇ 9 M for example 1 polymer and 1.58 ⁇ 10 ⁇ 10 M for example 9 polymer, respectively. The equilibrium constants (K) were then calculated as before. The concentration of trichloroethylene was determined by subtracting the amount of the trichloroethylene in the polymer from the initial concentration.
- An optical quality thin film of a cyclodextrin polymer similar to example 1 was prepared as follows. A flat round aluminum plate (a diameter of 1.3 inches and a thickness of 0.125 inches) was placed in the bottom of a 30-ml Teflon® beaker having a diameter of 1.5 inches. Dried ⁇ -cyclodextrin (0.4084 g, 0.360 mmol) was dissolved in 10 ml of dried DMF. Hexane-diisocyanate (0.55 ml; 2.879 mmol) was added into the solution. After stirring, the clear solution was poured into the Teflon® beaker with the aluminum plate as the support for the polymeric film.
- FIG. 2 shows a graph illustrating the second harmonic generation measurement for a free-standing film of the para-nitrophenol complex or reaction product with the diisocyanate crosslinked cyclodextrin polymer.
- Line 20 shows the results for the para-nitrophenol-cyclodextrin polymer complex, while line 22 shows the results for a quartz reference. The results of these measurements demonstrate that chromophore-cyclodextrin polymer complexes can have second order nonlinear optical properties.
- Dried gamma-cyclodextrin (2.0 g) was added to 20 ml of dried DMF, then 1,6-diisocynatohexane (2.2 ml) was added dropwise with vigorous stirring. Under a nitrogen atmosphere, the solution was heated at 85° C. for 1 day. A polymeric material was then recovered from the solution as a clear, transparent solid. Residual DMF was removed by heating under vacuum at 80° C. for 24 hours. The resultant product was a polymeric cyclodextrin solid which can be easily ground into powder.
- a measurement of the binding constant of the polymer from example 1 with methyl-nitrophenol (MNP) was conducted as follows. MNP (0.0034 g) was dissolved in 100 ml deionized water, and diluted at 5000 times to make a 4.4404 ⁇ 10 ⁇ 8 M solution. Polymer from example 1 (0.5867 g) was added to 1000 ml of the above solution. After 1 day, the yellow polymer was filtered off and washed with ethanol. Initial concentration of MNP 4.4404 ⁇ 10 ⁇ 8 M Final volume of ethanol 4.0 ml UV absorbance (A) 0.1702 Equilibrium Constant (K) 8.47 ⁇ 10 8 M ⁇ 1
- DMNP Dimethyl-nitrophenol
- HBS Hydroxybenzosulfunate
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Polyurethanes Or Polyureas (AREA)
Abstract
A water insoluble polymeric composition which is a reaction product of a cyclodextrin monomer and a polyfunctional crosslinker from the group of polyisocyanates, dihalohydrocarbons, and dihaloacetylhydrocarbons is disclosed together with a process for removing a target organic compound from an aqueous composition including contacting the aqueous composition containing a target organic compound with a water insoluble cyclodextrin polymer which is the reaction product of a cyclodextrin monomer and a polyfunctional crosslinker from the group of polyisocyanates, dihalohydrocarbons, and dihaloacetylhydrocarbons for time sufficient to form a reaction product between the water insoluble cyclodextrin polymer and the target organic compound whereby the concentration of the target organic compound in the aqueous composition is reduced. Organic chromophores added to the water insoluble cyclodextrin polymers can provide organic nonlinear optical materals.
Description
- This application claims the benefit of U.S. Provisional Application No. 60/031,645 filed Nov. 22, 1996.
- The present invention relates to cyclodextrin polymer materials and to the use of such cyclodextrin polymer materials as separation materials for separation or removal of, e.g., organic contaminants from aqueous compositions. More particularly, the present invention relates to water insoluble cyclodextrin polymer materials. This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).
- Various cyclodextrin polymers are known. Cserhati et al., Anal. Chim. Acta, vol. 279, pp. 107-113, 1993, describe monomer β-cyclodextrin polymerized on the surface of silica particles for use in a liquid chromatographic column and the retention characteristics of such a column with various ring-substituted phenol derivatives. Cserhati, Anal. Chim. Acta, vol. 292, pp. 17-22, 1994, describes a water insoluble β-cyclodextrin polymer formed by crosslinking β-cyclodextrin monomers with epichlorohydrin and ethyleneglycolbis(epoxypropyl ether). The resultant polymer was ground into a powder and thin layer chromatography plates were prepared with the powder. Binding properties of this β-cyclodextrin polymer with various esters of 3,5-dinitrobenzoic acid were studied. Kutner, Electrochimica Acta, vol.37, no. 6, pp. 1109-1117, 1992, describes α-cyclodextrin polymer films formed by crosslinking of a soluble α-cyclodextrin polymer (partially crosslinked with 1-chloro-2,3-epoxypropane) with glutaric aldehyde. The polymer films were studied in conjunction with a 4-nitrophenol/4-nitrophenolate guest system. Zhao et al., Reactive Polymers, vol. 24, pp. 9-16, 1994, describe β-cyclodextrin immobilized onto crosslinked styrene/divinylbenzene copolymer to form a β-cyclodextrin polymeric adsorbent. This adsorbent demonstrated apparent inclusion ability for isomeric compounds such as 2- and 4-nitro-substituted aromatic compounds, e.g., 2-nitrophenol, 4-nitrophenol and 2,4-dinitrophenol.
- Additionally, the use of cyclodextrin derivatives for adsorption or extraction of certain organic materials is known. For example, U.S. Pat. No. 5,190,663 uses cyclodextrin anchored to a water insoluble substrate or carrier particle to remove dissolved polynuclear aromatic hydrocarbons from an aqueous composition. U.S. Pat. No. 5,425,881 uses aqueous solutions of cyclodextrins or cyclodextrin derivatives in extraction of an organic pollutant from contaminated soil and also describes water soluble cyclodextrin polymers wherein the cyclodextrin is crosslinked with epichlorohydrin or isocyanate.
- Despite the previous work in the areas of cyclodextrin polymers and use of cyclodextrin materials for adsorption or extraction of organic pollutants, the area remains open to continued developments that can open up further opportunities.
- It is an object of the present invention to provide water insoluble cyclodextrin polymer materials.
- Another object of the invention is to provide water insoluble cyclodextrin polymer materials having a defined nanoporous structure.
- It is a further object of the invention to provide separation materials of water insoluble cyclodextrin polymers for the separation of selected target organic compounds, e.g., organic pollutants or contaminants, from aqueous compositions.
- It is a still further object of the invention to provide a process for separating target organic compounds, e.g., organic pollutants or contaminants, from an aqueous composition by contact with the presently disclosed water insoluble cyclodextrin polymer materials.
- To achieve the foregoing and other objects, and in accordance with the purposes of the present invention, as embodied and broadly described herein, the present invention provides a water insoluble polymeric composition comprising a reaction product of a cyclodextrin monomer and a polyfunctional crosslinker selected from the group consisting of polyisocyanates, dihalohydrocarbons, and dihaloacetylhydrocarbons.
- The present invention further provides a process for removing a target organic compound from an aqueous composition comprising contacting said aqueous composition containing a target organic compound with a water insoluble cyclodextrin polymer comprising a reaction product of a cyclodextrin monomer and a polyfunctional crosslinker selected from the group consisting of polyisocyanates, dihalohydrocarbons, and dihaloacetylhydrocarbons for time sufficient to form a reaction product between said water insoluble cyclodextrin polymer and said target organic compound whereby the concentration of said target organic compound in said aqueous composition is reduced.
- FIG. 1 is a graph showing induced circular dichroism of a cyclodextrin polymer complex with para-nitrophenol.
- FIG. 2 is a graph showing a second harmonic generation signal versus incident angle.
- FIG. 3 is a graph showing estimation on pore size by plotting actual loading of various organic materials having varying critical or maximum dimensions.
- The present invention is concerned with cyclodextrin polymers and to the use of such cyclodextrin polymers as separation materials for separating selected organic materials from aqueous streams or compositions.
- The cyclodextrin polymers of the present invention are generally formed by the reaction of a suitable cyclodextrin monomer with a polyfunctional crosslinking agent. The crosslinking agent may generally be an aromatic, an aliphatic or a cycloaliphatic polyfunctional crosslinking agent. Suitable polyfunctional crosslinking agents can include diisocyanates, polyisocyanates, dihalohydrocarbons, and dihaloacetylhydrocarbons. In addition, the polyfunctional crosslinking agent of the present invention can include asymmetric crosslinking agents containing different linking functionalities from among the functionalities of isocyanate, halo, or haloacetyl, on the linking molecule, e.g., at the ends of the molecule. An example of such a suitable asymmetric crosslinking agent may be 4-isocyanatobenzoyl chloride and the like. Preferably, the polyfunctional crosslinking agents include at least one isocyanate group or functionality.
- The cyclodextrin polymers of this invention are characterized as water insoluble. The term “water insoluble” is a relative term and as used herein generally refers to materials having a solubility in water of no greater than about 0.01 grams per gram of water. Further, the cyclodextrin polymers of this invention can have a nanoporous structure capable of absorbing selected target organic compounds from within aqueous streams, solutions or compositions down to levels as low as parts per billion (ppb) and even to levels of parts per trillion (ppt).
- Diisocyanates can include such as 2,6-tolylene diisocyanate, 2,4-tolylene diisocyanate (TDI), 4,4′-diphenylmethane diisocyanate (MDI), hexamethylene diisocyanate (HDI), and the like.
- Dihalohydrocarbons can be generally represented by the formula X-R1-X where X is a halogen selected from among chlorine, bromine and iodine, preferably chlorine, and R1 is an alkylene group such as propylene, butylene, pentylene, hexylene, heptylene, octylene and the like, an alkylaryl group such as dimethylenebenzene, dipropylenebenzene and the like. Specific examples of suitable dihalohydrocarbons may include 1,3-dichloropropane, 1,3-dibromopropane, 1,3-diiodopropane, 1,6-dichlorohexane, 1,6-dibromohexane, 1,6-diiodohexane, 1,8-dichlorooctane, 1,8-dibromooctane, 1,8-diiodooctane, 1,4-chloromethylenebenzene, 1,4-bromomethylenebenzene, and 1,4-iodomethylenebenzene.
- Dihaloacetylhydrocarbons can be generally represented by the formula XOC-R2-COX where X is a halogen selected from among chlorine, bromine and iodine, preferably chlorine, and R2 is an alkylene group such as propylene, butylene, pentylene, hexylene, heptylene, octylene and the like, an alkylaryl group such as dimethylenebenzene, dipropylenebenzene and the like. Suitable dihaloacetylhydrocarbons may be generally prepared by chlorination of dibasic acids such as dicarboxylic acids and specific examples of dicarboxylic acids may include 1,4-butanedicarboxylic acid (adipic acid), ortho-benzene dicarboxylic acid (oxalic acid), cis-butenedioic acid (maleic acid), and decanedioic acid (sebacic acid).
- Suitable cyclodextrin monomer materials include α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin or substituted α-cyclodextrins, substituted β- cyclodextrins, or substituted γ-cyclodextrins, preferably substituted α-cyclodextrins, substituted β-cyclodextrins, or substituted γ-cyclodextrins. Generally, cyclodextrins are linked D-glucopyranose units, with α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin being composed of 6, 7, or 8 units, respectively, the units linked into a circular arrangement. Accordingly, the internal diameter of each of α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin varies from the others. α-cyclodextrin has a cavity size or internal diameter of about 4.7 to 5.2 Angstroms (A), β-cyclodextrin has an internal diameter of about 6.0 to 6.5A, and γ-cyclodextrin has an internal diameter of about 7.5 to 8.5A. Branched cyclodextrin monomer materials may also be employed.
- The term “substituted cyclodextrin” refers to a cyclodextrin modified by the addition of other functional groups, e.g., a cyclodextrin wherein a hydrogen atom of one or more primary or secondary hydroxyl groups therein has been substituted by, e.g., a carboxyl group, a carboxyl alkyl group, a carboxylaryl group, an alkyl group, e.g., either a lower alkyl such as a C1 to C4 group, i.e., methyl, ethyl, propyl or butyl, or a longer chain aliphatic containing from about 8 to about 22 carbons, a hydroxyalkyl group, a sulfonic group, or an alkylenesulfonic group and the like. Modification of a cyclodextrin can alter the length and size of the internal cavity or alter the chemical compatibility or binding properties of the particular substituted cyclodextrin with a target organic compound.
- One manner of preparing a substituted cyclodextrin polymer may be to modify or fuctionalize a cyclodextrin monomer prior to polymerization of the monomer. Another manner of preparing a substituted cyclodextrin polymer may be to polymerize a cyclodextrin monomer and then to modify or functionalize the resultant cyclodextrin polymer. Preferably, the substituted cyclodextrin monomer is prepared prior to polymerization of the substituted cyclodextrin monomer. One benefit of tailoring the substituted cyclodextrin functionality may be to alter the retention times of the particular target organic species.
- The process of the present invention is characterized by the feature that the initial concentration of the target organic compounds in the aqueous composition is generally relatively low and the final concentration of the target organic compounds after treatment with the cyclodextrin polymers of the present invention is extremely low.
- The cyclodextrin polymers and the substituted cyclodextrin polymers of the present invention have been found to be selective for the target organic compounds and can generally effect essentially complete removal of such target organic compounds contained within a sample of water so long as the concentration of organic compounds is not so great to exceed the amount of cyclodextrin polymer material used.
- In the process of the present invention, an aqueous composition including a relatively low concentration of target organic compounds is contacted with a water insoluble cyclodextrin polymer comprising the reaction product of a cyclodextrin monomer and a polyfunctional crosslinker selected from among polyisocyanates, dihalohydrocarbons, and dihaloacetylhydrocarbons. Asymmetrical crosslinkers may also be employed. Typically, the process is carried out under conditions such as at temperatures and for periods of time sufficient to reduce the amount of target organic compounds to a preselected level. The entire operation can be carried out at ambient conditions in which case complexation of the target organic compounds is fairly rapid, and contact times between the water insoluble cyclodextrin polymer and the aqueous composition can be as short as about five seconds or less. Increasing the contact time has no detrimental effect on the process and may in fact increase removal efficiency of the target organic compounds. There is a tradeoff between the process conditions and the amount of water insoluble cyclodextrin polymer used. There is a general stoichiometric reaction between cyclodextrins and target organic compounds. The reaction is first order, i.e., the rate correlates with concentration and surface area. Increasing the surface area or organic concentration will increase reaction rate. Increasing temperature will also increase the reaction rate of removal of organics. Thus, if complexation is inhibited by rapid contact rates, high temperatures or when purifying an aqueous stream containing high hydrocarbon levels, increasing the amount of water insoluble cyclodextrin polymer generally increases removal efficiency of the target organic compounds.
- The process of the present invention can be conducted by using a powder of the water insoluble cyclodextrin polymer where such powder is supported in a packed column, cartridge, or bed through which the aqueous composition is passed at a suitable rate to effect removal of the target organic compounds. In another embodiment of the process of the invention, a larger piece (as opposed to a powder) of the water insoluble cyclodextrin polymer, e.g., a piece having dimensions of at least about one quarter inch by about one quarter inch by about one quarter inch, or a spherical piece having a diameter of at least about one quarter inch, can be placed in contact with a quantity of water containing a undesirable amount of the target organic compounds. The specific size of the larger piece is not critical and smaller pieces, greater than a typical powder, can be used singly or in combination with other small pieces to extract target organic compounds from a water sample such as a well and the like. Generally, such an undesirable amount of the target organic compounds is an amount exceeding some defined level such as levels set by agencies such as the United States Environmental Protection Agency (EPA). Generally, the concentration of the target organic compounds will normally be reduced to a level which is prescribed for such pollutants or to a level lower than present conventional detection limits. In yet another embodiment of the process of the invention, a thin film of the water insoluble cyclodextrin polymer can be formed on a support substrate such as a glass substrate, or on beads, and the supported thin film of the water insoluble cyclodextrin polymer contacted with the aqueous stream including the target organic compounds. Such a thin film can typically be of a thickness from about 0.01 microns to about 5 millimeters. Hollow fibers of the cyclodextrin polymer may also be employed.
- After separation of the target organic compounds from an aqueous composition by the water insoluble cyclodextrin polymer, the target organic compounds can be separated from the water insoluble cyclodextrin polymer by extraction with a suitable extraction agent or solvent. Suitable extraction agents or solvents can include alcohols such as methanol, ethanol and the like.
- Among the various organic compounds that can be target materials for removal from aqueous streams are included aromatic compounds, e.g., benzene, toluene, xylene and the like, polyaromatic compounds including compounds with fused ring structures containing between about two and ten rings, some or all of which are benzene rings, e.g., naphthalenes, indenes, anthracenes, phenanthrenes, fluorenes, acenaphthenes, benzanthracenes, perylenes, tetracenes, pyrenes, benzopyrenes, benzoperylenes, and the like, oxygen-containing organic compounds, e.g., methanol, acetone, dimethyl sulfoxide, dimethyl formamide, tetrahydrofuran and the like, halogenated, e.g., brominated or chlorinated, hydrocarbons, e.g., chloroform, carbon tetrachloride, methylene chloride, trichloroethane, tetrachloroethene, dichloroethylene, trichloroethylene, and the like, and nitro-containing compounds, e.g., para-nitrophenol, nitrobenzene, dinitrobenzene, trinitrobenzene, hexanitrobenzene, nitrotoluene, dinitrotoluene and the like. Clean-up of explosive materials may be carried out with the polymer materials of this invention as such explosive materials are generally nitro-containing organic compounds.
- The ability of the present cyclodextrin polymers to serve as separation materials can be compared with other conventional separation type materials such as activated carbon and zeolites (molecular sieves). The following table shows a comparison of a diisocyanate crosslinked β-cyclodextrin polymer with para-nitrophenol as the target organic.
TABLE 1 Type of separation material zeolite characteristic of separation material CD polymer activated carbon (3A) Pore diameter (Å)* 7-9 — 3 Surface area (square meters per gram) 1.7-1.9 750 — Binding (Formation) constants with 6.9 × 109 M−1 1.4 × 104 M−1 ≈0.0 M−1 organic material Total absorbance of organic material 22 mg/gram 58 mg/gram ≈0.0 (Loading level) Effective clean-up limits in water ≈3.0 ppt ≈1.3 ppm — (equilibrium concentration) Leachability of organic material in water No Yes Yes Formability into a thin film or Yes No No membrane Absorption of water in air No 19.6 mg/hr.g Yes - It can be seen that the cyclodextrin polymer can lower the concentration of some organic materials to as low as about 3 parts per trillion (ppt), far lower than a conventional separation material of activated carbon at about 1.3 parts per million (ppm). Activated carbon is often used in typical pump and treat systems for groundwater contamination. While activated carbon has a higher loading capacity at 58 milligrams per gram than the cyclodextrin polymer, the activated carbon can be leached by additional water to contaminate further water whereas the cyclodextrin polymer will bind the target organic until eluation with some non-aqueous solvent such as ethanol.
- In addition to the use of the present water insoluble cyclodextrin polymers as separation materials for selected organic compounds, it has been found that the reaction product (often referred to as a complexation product) of certain organic compounds with the water insoluble cyclodextrin polymer can have nonlinear optical properties such that the reaction product can be characterized as an organic nonlinear optical material. Optical quality thin films can be prepared from the water insoluble cyclodextrin polymers of the present invention. Such thin films can then absorb organic chromophores from water. Some chromophores can be nonlinear optical materials, typically for polar molecules. Among suitable chromophores may be included 4-nitrophenol, 4-nitrostyryl-4′-phenol, 4-hydroxylstilbazole, and 4-hydroxylstilbazolium iodide. Such polar molecules will have a preferential orientation inside the cavity of a cyclodextrin material since the polar nature of the water-polymer interface will generally cause the chromophore to orient prior to entering the polymeric matrix. Then, once the chromophore enters the solid cyclodextrin polymer material, the chromophores retain the alignment and can possess second order nonlinear optical properties. Organic nonlinear optical materials offer potential for use in integrated optical devices.
- The present invention is more particularly described in the following examples which are intended as illustrative only, since numerous modifications and variations will be apparent to those skilled in the art.
- To 2.0 grams (g) of dried β-cyclodextrin (β-CD) in 10 milliliters (ml) of dried dimethylformamide (DMF), 1,6-diisocyanatohexane (HDI) was added dropwise with vigorous stirring. The total volume of HDI added was 2.5 ml. Under a nitrogen atmosphere, the solution was heated at 80° C. for 16 hours. A polymeric material was then recovered from the solution as a clear, transparent solid. Residual DMF was removed by heating under vacuum at 80° C. for 24 hours. The resultant product was a polymeric cyclodextrin solid which could easily be ground into a powder.
- To 2.0 g of dried α-cyclodextrin (α-CD) in 10 ml of dried DMF, 1,6-diisocyanatohexane (HDI) was added dropwise with vigorous stirring. The total volume of HDI added was 2.5 ml. Under a nitrogen atmosphere, the solution was heated at 80° C. for 16 hours. A polymeric material was then recovered from the solution as a clear, transparent solid. Residual DMF was removed by heating under vacuum at 80° C. for 24 hours. As in example 1, the resultant product was a polymeric cyclodextrin solid which could easily be ground into a powder.
- To 2.0 g of dried β-cyclodextrin (β-CD) in 10 ml of dried DMF, toluene 2,4-diisocyanate (TDI) was added dropwise with vigorous stirring. The total volume of TDI added was 2.5 ml. Under a nitrogen atmosphere, the solution was heated at 80° C. for 16 hours. A polymeric material was then recovered from the solution as a clear, transparent solid. Residual DMF was removed by heating under vacuum at 80° C. for 24 hours. As in example 1, the resultant product was a polymeric cyclodextrin solid which could easily be ground into a powder.
- To 2.0 g of dried α-cyclodextrin (α-CD) in 10 ml of dried DMF,
toluene 1,6-diisocyanate (TDI) was added dropwise with vigorous stirring. The total volume of TDI added was 2.5 ml. Under a nitrogen atmosphere, the solution was heated at 80° C. for 16 hours. A polymeric material was then recovered from the solution as a clear, transparent solid. Residual DMF was removed by heating under vacuum at 80° C. for 24 hours. As in example 1, the resultant product was a polymeric cyclodextrin solid which could easily be ground into a powder. - To 2.0 g of dried β-cyclodextrin (β-CD) in 10 ml of dried DMF, 1,6-diisocyanatodecane (DDI) was added dropwise with vigorous stirring. The total volume of DDI added was 2.5 ml. Under a nitrogen atmosphere, the solution was heated at 80° C. for 16 hours. A polymeric material was then recovered from the solution as a clear, transparent solid. Residual DMF was removed by heating under vacuum at 80° C. for 24 hours. As in example 1, the resultant product was a polymeric cyclodextrin solid which could easily be ground into a powder.
- The reactivity of the bi-functional linkers, i.e., the HDI, TDI and DDI was observed to be: TDI>HDI>DDI. The hydrophobicity of the resulting cyclodextrin polymers varied with the bi-functional linker with DDI>HDI>TDI. In each of example 1-5, infrared measurements indicated that the isocyanato groups had disappeared and peaks corresponding to O—C═O, O═C—NH and NH groups were observed.
- A water solution, total volume 4.16 liters, containing about 3×10−9 moles per liter (M) of para-nitrophenol was passed through a glass column packed with 0.5858 g of powder of the cyclodextrin polymer from example 1. The powder gradually turned visibly yellow in color from its initial clear, colorless appearance. Retention of para-nitrophenol by the cyclodextrin polymer powder was confirmed. The final solution concentration of para-nitrophenol was measured as 1.44×10−10 M. The para-nitrophenol was then separated from the cyclodextrin polymer powder by washing of the cyclodextrin polymer powder with ethanol. The non-covalent binding of the para-nitrophenol to the cyclodextrin polymer powder allowed the separation of the para-nitrophenol from the cyclodextrin polymer powder by washing with an organic solvent such as ethanol.
- Formation constants were calculated as 6.93×10−9 M−1 for the HDI-β-CD/para-nitrophenol complex and as 1.64×109 M−1 for the TDI-β-CD/ para-nitrophenol complex.
- A sample of the resultant product between the HDI-β-CD and the para-nitrophenol was measured and contrasted with a sample of the HDI-β-CD. Measurements for induced circular dichroism are shown in FIG. 1 where
solid line 10 shows the plot for the resultant product between the HDI-β-CD and the para-nitrophenol while dashedline 12 shows the plot for the sample of HDI-β-CD. The peak inline 10 at about 400 nanometers (nm) indicates the induced circular dichroism due to complex formation. - A bulk portion of the polymer from example 1 was immersed in a one liter water solution containing about 2×10−7 M of para-nitrophenol for one day. The solid polymer (about 0.5 g) became visibly yellow after which it was removed from the solution. The final solution concentration of para-nitrophenol was measured as 1.8×10−10 M. The solid was then washed with ethanol whereupon para-nitrophenol was removed from the solid polymer until it again appeared clear and colorless.
- Synthesis of a substituted cyclodextrin was as follows. Dried β-cyclodextrin (1.3476 g; 1.187 mmole) was dissolved in 25 ml of dried DMSO. Sodium hydride (0.1996 g; 8.309 mmole) was added and the mixture was stirred at ambient temperature for 20 minutes. Then the mixture was cooled to 0° C. and 1.1797 ml (8.309 mmole) of methyl iodide was added dropwise over a period of 5 minutes. The mixture was stirred at room temperature for 24 hours. The excess of sodium hydride was decomposed by addition of 20 ml methanol. By pouring the solution into 200 ml of ice water, the product was precipitated from the solution and dried in vacuum for 24 hours.
- The methyl-substituted cyclodextrin was then polymeric with HDI as follows. The methyl substituted β-cyclodextrin monomer from example 8, i.e., CD-OCH3
- (0.5720 g; 0.4587 mmole), was dissolved in 10 ml of dried DMF. Hexane-diisocyanate (HDI) (1.10 ml; 3.67 mmole) was added dropwise to the solution. After the addition of HDI, the mixture was heated up to 85° C. and stirred for 2 days. The solvent was removed by distillation in vacuum for 1 day. The dried polymer product was grounded into powder.
- A measurement of the binding constant of the polymer from example 9 with toluene was conducted as follows. A standard solution of toluene in ethanol (2.345×10−3M) was prepared and calibrated by UV measurements. Absorbance was 0.973 at λ=262 nanometers (nm). Exactly 5 ml of this standard toluene solution was diluted to 1000 ml with water in order to obtain a toluene in water solution with a concentration of 4.6939×10−7 M.
- Binding or equilibrium constant (K) measurements were as follows. The polymers (0.8292 g for the polymer of example 1 and 0.8751 gram for the polymer of example 9) were each immersed in 1000 ml of the aqueous toluene solution (4.6939×10−7 M) and stirred for 1 day. The final toluene concentration in the water was 8.85×10−9 M for the polymer of example 1 and 3.76×10−9 M for the polymer of example 9, respectively. Then the polymer was filtered off and washed with ethanol. The volume of ethanol was concentrated to around 5 ml.
- The equilibrium constants (K) were then calculated with use of the following formula.
- K=1/[organic compound]M
- The concentration of toluene was determined by subtracting the amount of the toluene in the polymer from the initial concentration. The amount of organic in the individual polymers was eluted from the polymer, concentrated in ethanol solution, and measured accurately by UV absorption. The following equilibrium constants were obtained:
example 1 polymer example 9 polymer Final Volume of ethanol 3.9 ml 4.6 ml UV absorbance (A) 0.049 0.042 Equilibrium Constant K = 1.13 × 108 M−1 2.66 × 108 M−1 - A measurement of the binding constant of the polymer from example 9 with trichloroethylene (TCE) was conducted as follows. Exactly 2 ml of TCE was added to 1000 ml of water in a separation funnel to make the saturated TCE-H2O solution. This solution was diluted by taking 20 ml of this saturated water solution from the aqueous phase and diluting to 2000 ml. The TCE concentration of this water solution was calibrated against a standard solution of TCE-hexane using UV absorption at 218 nm. The final aqueous concentration of TCE was determined to be 7.589×10−8 M.
- Binding or equilibrium constant (K) measurements were as follows. The polymers (0.8818 g for example 1 polymer; 0.8008 gram for example 9 polymer) were immersed in 2000 ml of the aqueous TCE solution (7.589×10−8 M) and stirred for 1 day. The final TCE concentration in the water was found to be 1.05×10−9 M for example 1 polymer and 1.58×10−10 M for example 9 polymer, respectively. The equilibrium constants (K) were then calculated as before. The concentration of trichloroethylene was determined by subtracting the amount of the trichloroethylene in the polymer from the initial concentration. The amount of organic in the individual polymers was eluted from the polymer, concentrated in ethanol solution, and measured accurately by UV absorption. The following equilibrium constants were obtained:
example 1 polymer example 9 polymer Concentration 7.5892 × 10−8 M 7.5892 × 10−8 M of initial TCE solution Final Volume of ethanol 8.0 ml 5.5 ml UV absorbance (A) 0.089 0.131 Equilibrium constant 9.52 × 108 M−1 6.32 × 109 M−1 - An optical quality thin film of a cyclodextrin polymer similar to example 1 was prepared as follows. A flat round aluminum plate (a diameter of 1.3 inches and a thickness of 0.125 inches) was placed in the bottom of a 30-ml Teflon® beaker having a diameter of 1.5 inches. Dried β-cyclodextrin (0.4084 g, 0.360 mmol) was dissolved in 10 ml of dried DMF. Hexane-diisocyanate (0.55 ml; 2.879 mmol) was added into the solution. After stirring, the clear solution was poured into the Teflon® beaker with the aluminum plate as the support for the polymeric film. Then the whole beaker was put into a glass container which had been pre-heated to 60° C. The container was kept in the oil bath at constant temperature 60° C. for 1 day. A transparent colorless film with the thickness around {fraction (1/16)} inches was formed on the aluminum plate.
- This optical quality film absorbed para-nitrophenol from a water solution. The para-nitrophenol served as an organic chromophore. These polar molecules are believed to have preferentially oriented themselves inside the cyclodextrin polymer because the polar nature of water-polymer interface causes the chromophore to orient before entering the polymeric matrix. FIG. 2 shows a graph illustrating the second harmonic generation measurement for a free-standing film of the para-nitrophenol complex or reaction product with the diisocyanate crosslinked cyclodextrin polymer.
Line 20 shows the results for the para-nitrophenol-cyclodextrin polymer complex, while line 22 shows the results for a quartz reference. The results of these measurements demonstrate that chromophore-cyclodextrin polymer complexes can have second order nonlinear optical properties. - Dried gamma-cyclodextrin (2.0 g) was added to 20 ml of dried DMF, then 1,6-diisocynatohexane (2.2 ml) was added dropwise with vigorous stirring. Under a nitrogen atmosphere, the solution was heated at 85° C. for 1 day. A polymeric material was then recovered from the solution as a clear, transparent solid. Residual DMF was removed by heating under vacuum at 80° C. for 24 hours. The resultant product was a polymeric cyclodextrin solid which can be easily ground into powder.
- A measurement of the binding constant of the polymer from example 1 with methyl-nitrophenol (MNP) was conducted as follows. MNP (0.0034 g) was dissolved in 100 ml deionized water, and diluted at 5000 times to make a 4.4404×10−8 M solution. Polymer from example 1 (0.5867 g) was added to 1000 ml of the above solution. After 1 day, the yellow polymer was filtered off and washed with ethanol.
Initial concentration of MNP 4.4404 × 10−8 M Final volume of ethanol 4.0 ml UV absorbance (A) 0.1702 Equilibrium Constant (K) 8.47 × 108 M−1 - Dimethyl-nitrophenol (DMNP) (0.0047 g) was dissolved in 250 ml deionized water and diluted 1000 times to make a 8.9974×10−9 M solution. Polymer from example 1 (0.6435 g) was added into 1000 ml of the above solution. After 1 day, the yellow polymer was filtered off and washed with ethanol.
Initial concentration of DMNP 8.9974 × 10−9 M Final volume of ethanol 3.8 ml UV absorbance (A) 0.01414 Equilibrium Constant (K) 1.53 × 108 M−1 - Hydroxybenzosulfunate (HBS) (0.0027 g) was dissolved in 500 ml deionized water and diluted 1000 times to make a 2.3257×10−8 M solution. Polymer from example 1 (0.6626 g) was added into 1000 ml of the above solution. After 1 day, the yellow polymer was filtered off and washed with ethanol.
Initial concentration of DMNP 2.3257 × 10−8 M Final volume of ethanol 4.0 ml UV absorbance (A) 0.1286 Equilibrium Constant (K) 4.23 × 108 M−1 - Additional testing yielded the following data shown in Tables 2 and 3.
TABLE 2 CD-HDI CD-TDI Me-CD-HDI K Mass Load Limit in K K Organics (1/M) Loading % (mg) water (1/M) (1/M) 4-Nitrophenol 6.9 × 109 86 22 3 ppt 2.2 × 109 TCE 9.5 × 108 85 19 19 ppt 6.3 × 109 Toluene 1.1 × 108 80 14 0.2 ppb 2.7 × 108 Phenol 8.0 × 107 82 14 0.2 ppb -
TABLE 3 Mass K Loading Limit in Organics (1/M) Loading % (mg) Water Methyl-nitrophenol 8.5 × 108 82 23 21 ppt Dimethyl-nitro 1.5 × 108 82 25 0.1 ppb phenol 2-Nitro-1- 1.1 × 108 80 28 0.2 ppb naphthol 4-Nitrothio- 2.7 × 109 85 24 7 ppt phenol 4-Hydroxybenzene- 4.2 × 108 82 29 42 ppt sulfonic acid 4-Hydroxy-1- ˜1 × 108 naphthalene- (not stable) sulfonic acid 1,3,6,8-Pyrene- 7.0 × 107 54 60 26 ppb tetra-sulfonic acid - Although the present invention has been described with reference to specific details, it is not intended that such details should be regarded as limitations upon the scope of the invention, except as and to the extent that they are included in the accompanying claims.
Claims (14)
1. A water insoluble polymeric composition comprising a reaction product of a cyclodextrin monomer and a polyfunctional crosslinker selected from the group consisting of polyisocyanates, dihalohydrocarbons, and dihaloacetylhydrocarbons.
2. The water insoluble polymeric composition of wherein said cyclodextrin monomer is selected from the group consisting of α-cyclodextrin, substituted α-cyclodextrin, β-cyclodextrin, substituted β-cyclodextrin, γ-cyclodextrin, and substituted γ-cyclodextrin.
claim 1
3. The water insoluble polymeric composition of wherein said polyfunctional crosslinker is a polyisocyanate.
claim 1
4. The water insoluble polymeric composition of wherein said polyisocyanate crosslinker is selected from the group consisting of aromatic diisocyanates and diisocyanatoalkanes.
claim 3
5. The water insoluble polymeric composition of wherein at least one hydroxyl group on said cyclodextrin monomer is substituted with an alkyl group to form one or more alkoxide groups.
claim 1
6. A process for removing a target organic compound from an aqueous composition comprising:
contacting said aqueous composition containing a target organic compound with a water insoluble cyclodextrin polymer comprising a reaction product of a cyclodextrin monomer and a polyfunctional crosslinker selected from the group consisting of polyisocyanates, dihalohydrocarbons, and dihaloacetylhydrocarbons for time sufficient to form a reaction product between said water insoluble cyclodextrin polymer and said target organic compound whereby the concentration of said target organic compound in said aqueous composition is reduced.
7. The process of wherein said water insoluble cyclodextrin polymer is contacted with said aqueous composition by passing said aqueous composition through a fixed bed of particles of said water insoluble cyclodextrin polymer.
claim 6
8. The process of wherein said water insoluble cyclodextrin polymer contacted with said aqueous composition is a solid with dimensions of at least about one quarter inch by one quarter inch by one quarter inch.
claim 6
9. The process of wherein said water insoluble cyclodextrin polymer contacted with said aqueous composition is in the form of a porous membrane or hollow fiber.
claim 6
10. The process of wherein said water insoluble cyclodextrin polymer contacted with said aqueous composition is a thin film.
claim 6
11. The process of wherein the fixed bed of particles is in a cartridge.
claim 7
12. A nonlinear optical material comprising:
a defined substrate;
a thin film of a reaction product between an organic chromophore and a water insoluble cyclydextrin polymeric composition.
13. The nonlinear optical material of wherein said water insoluble cyclydextrin polymeric composition is a reaction product of a cyclodextrin monomer and a polyfunctional crosslinker selected from the group consisting of polyisocyanates, dihalohydrocarbons, and dichloroacetylhydrocarbons.
claim 12
14. The nonlinear optical material of wherein said organic chromophore is selected from the group consisting of 4-nitrophenol, 4-nitrostyryl-4′-phenol, 4-hydroxylstilbazole, and 4-hydroxylstilbazolium iodide.
claim 12
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/214,216 US20010008222A1 (en) | 1998-12-23 | 1997-11-21 | Cyclodextrin polymer separation materials |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/214,216 US20010008222A1 (en) | 1998-12-23 | 1997-11-21 | Cyclodextrin polymer separation materials |
Publications (1)
Publication Number | Publication Date |
---|---|
US20010008222A1 true US20010008222A1 (en) | 2001-07-19 |
Family
ID=22798241
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09/214,216 Abandoned US20010008222A1 (en) | 1998-12-23 | 1997-11-21 | Cyclodextrin polymer separation materials |
Country Status (1)
Country | Link |
---|---|
US (1) | US20010008222A1 (en) |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030146172A1 (en) * | 2002-02-05 | 2003-08-07 | Mark Brusseau | Extraction of pollutants from underground water |
US20080194784A1 (en) * | 2006-11-29 | 2008-08-14 | Man Ho Choi | Entrapped Beta-Cyclodextrin Polymer and a Method of Preparing the Same |
WO2009003656A1 (en) * | 2007-07-04 | 2009-01-08 | Sea Marconi Technologies Di Vander Tumiatti S.A.S. | Cyclodextrin-based nanosponges as a vehicle for antitumoral drugs |
WO2013059285A1 (en) * | 2011-10-17 | 2013-04-25 | Temple University-Of The Commonwealth System Of Higher Education | Silica particles coated with beta-cyclodextrin for the removal of emerging contaminants from wastewater |
KR101437252B1 (en) | 2010-02-16 | 2014-09-02 | 네오스 컴파니 리미티드 | Agent and method for selectively anchoring halogenated aromatic compound contained in medium |
CN107163226A (en) * | 2017-06-20 | 2017-09-15 | 吉林省爱诺德生物工程有限公司 | A kind of preparation method of Ochratoxin A molecularly imprinted polymer |
CN107226900A (en) * | 2017-06-20 | 2017-10-03 | 吉林省爱诺德生物工程有限公司 | A kind of preparation method of aflatoxin B1 molecularly imprinted polymer |
CN107709251A (en) * | 2015-06-16 | 2018-02-16 | 安捷伦科技有限公司 | For analyzing the composition and method of sample preparation |
WO2020168104A1 (en) * | 2019-02-14 | 2020-08-20 | Cyclopure, Inc. | Charge-bearing cyclodextrin polymeric materials and methods of making and using same |
US20200262936A1 (en) * | 2019-02-14 | 2020-08-20 | Cyclopure, Inc. | Charge-bearing cyclodextrin polymeric materials and methods of making and using same |
-
1997
- 1997-11-21 US US09/214,216 patent/US20010008222A1/en not_active Abandoned
Cited By (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6787038B2 (en) * | 2002-02-05 | 2004-09-07 | Cerestar Holding B.V. | Extraction of pollutants from underground water |
US20030146172A1 (en) * | 2002-02-05 | 2003-08-07 | Mark Brusseau | Extraction of pollutants from underground water |
US20080194784A1 (en) * | 2006-11-29 | 2008-08-14 | Man Ho Choi | Entrapped Beta-Cyclodextrin Polymer and a Method of Preparing the Same |
US7745558B2 (en) * | 2006-11-29 | 2010-06-29 | Korea Institute Of Science And Technology | Entrapped β-cyclodextrin polymer and a method of preparing the same |
WO2009003656A1 (en) * | 2007-07-04 | 2009-01-08 | Sea Marconi Technologies Di Vander Tumiatti S.A.S. | Cyclodextrin-based nanosponges as a vehicle for antitumoral drugs |
KR101437252B1 (en) | 2010-02-16 | 2014-09-02 | 네오스 컴파니 리미티드 | Agent and method for selectively anchoring halogenated aromatic compound contained in medium |
US9828458B2 (en) | 2011-10-17 | 2017-11-28 | Temple University—Of the Commonwealth System of Higher Education | Silica particles coated with β-cyclodextrin for the removal of emerging contaminants from wastewater |
WO2013059285A1 (en) * | 2011-10-17 | 2013-04-25 | Temple University-Of The Commonwealth System Of Higher Education | Silica particles coated with beta-cyclodextrin for the removal of emerging contaminants from wastewater |
CN107709251A (en) * | 2015-06-16 | 2018-02-16 | 安捷伦科技有限公司 | For analyzing the composition and method of sample preparation |
US10564076B2 (en) * | 2015-06-16 | 2020-02-18 | Agilent Technologies, Inc. | Compositions and methods for analytical sample preparation |
CN107226900A (en) * | 2017-06-20 | 2017-10-03 | 吉林省爱诺德生物工程有限公司 | A kind of preparation method of aflatoxin B1 molecularly imprinted polymer |
CN107163226A (en) * | 2017-06-20 | 2017-09-15 | 吉林省爱诺德生物工程有限公司 | A kind of preparation method of Ochratoxin A molecularly imprinted polymer |
WO2020168104A1 (en) * | 2019-02-14 | 2020-08-20 | Cyclopure, Inc. | Charge-bearing cyclodextrin polymeric materials and methods of making and using same |
US20200262936A1 (en) * | 2019-02-14 | 2020-08-20 | Cyclopure, Inc. | Charge-bearing cyclodextrin polymeric materials and methods of making and using same |
US11001645B2 (en) * | 2019-02-14 | 2021-05-11 | Cyclopure, Inc. | Charge-bearing cyclodextrin polymeric materials and methods of making and using same |
CN113056516A (en) * | 2019-02-14 | 2021-06-29 | 赛克洛珀股份有限公司 | Charged cyclodextrin polymer materials and methods of making and using the same |
US11155646B2 (en) | 2019-02-14 | 2021-10-26 | Cyclopure, Inc. | Charge-bearing cyclodextrin polymeric materials and methods of making and using same |
JP2022519823A (en) * | 2019-02-14 | 2022-03-25 | サイクロピュア, インコーポレイテッド | Charged cyclodextrin polymer material and its manufacturing method and usage |
US11512146B2 (en) | 2019-02-14 | 2022-11-29 | Cyclopure, Inc. | Charge-bearing cyclodextrin polymeric materials and methods of making and using same |
JP7403855B2 (en) | 2019-02-14 | 2023-12-25 | サイクロピュア, インコーポレイテッド | Charged cyclodextrin polymer materials and methods of making and using them |
US11965042B2 (en) | 2019-02-14 | 2024-04-23 | Cyclopure, Inc. | Charge-bearing cyclodextrin polymeric materials and methods of making and using same |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO1998022197A9 (en) | Cyclodextrin polymer separation materials | |
EP0939667A1 (en) | Cyclodextrin polymer separation materials | |
US20010008222A1 (en) | Cyclodextrin polymer separation materials | |
Pellicer et al. | Removing of Direct Red 83: 1 using α-and HP-α-CDs polymerized with epichlorohydrin: Kinetic and equilibrium studies | |
Landy et al. | Remediation technologies using cyclodextrins: an overview | |
Ozmen et al. | Use of β-cyclodextrin and starch based polymers for sorption of Congo red from aqueous solutions | |
Sar et al. | Synthesis of glutamic acid derived organogels and their applications in dye removal from aqueous medium | |
US4917956A (en) | Method of preparing cyclodextrin-coated surfaces | |
US3754055A (en) | Cationic vinyl-pyridine copolymers and products thereof | |
Biyani et al. | Light-stimulated mechanically switchable, photopatternable cellulose nanocomposites | |
Wang et al. | Carbon-dot hydrogels as superior carbonaceous adsorbents for removing perfluorooctane sulfonate from water | |
Riaz et al. | Semi-conducting poly (1-naphthylamine) nanotubes: A pH independent adsorbent of sulphonate dyes | |
Sultan et al. | Linear and crosslinked Polyurethanes based catalysts for reduction of methylene blue | |
Teixeira et al. | Comparative study of PEO and PVA hydrogels for removal of methylene blue dye from wastewater | |
Danquah et al. | Phenolic pollutant uptake properties of molecular templated polymers containing β-cyclodextrin | |
Gong et al. | Adsorption property and mechanism of polyacrylate-divinylbenzene microspheres for removal of trace organic micropollutants from water | |
Chen et al. | Synthesis, characterization and antibacterial activity of novel β-cyclodextrin polyurethane materials | |
Zhang et al. | Porous hybrid triazine-functionalized polymers with high capture ability for I2 and dyes | |
Crini et al. | Sorption properties toward substituted phenolic derivatives in water using macroporous polyamines containing β‐cyclodextrin | |
He et al. | Highly efficient organic dyes capture using thiol-functionalized porous organic polymer | |
Sawada et al. | Vinylic Rotaxane Cross‐Linker Comprising Different Axle Length for the Characterization of Rotaxane Cross‐linked Polymers | |
Dominguez et al. | Anion exchange fibers for arsenate removal derived from a vinylbenzyl chloride precursor | |
CS254291A3 (en) | Method of enlarging surface area of an adsorption aromatic copolymer | |
US5037857A (en) | High-surface-area fiber | |
Surikumaran et al. | Synthesis and evaluation of methacrylic acid functionalized β-cyclodextrin based molecular imprinted polymer for 2, 4-dichlorophenol in water samples |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE, NEW Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:MA, MIN;LI, DEQUAN;REEL/FRAME:010136/0743 Effective date: 19981218 |
|
AS | Assignment |
Owner name: APPLIGENE-ONCOR S.A., FRANCE Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:QUERELLOU, JOEL;CAMBON, MARIE ANNE;REEL/FRAME:010300/0211 Effective date: 19990413 |
|
STCB | Information on status: application discontinuation |
Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION |