US1482966A - Bacteriological test apparatus - Google Patents

Bacteriological test apparatus Download PDF

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US1482966A
US1482966A US570749A US57074922A US1482966A US 1482966 A US1482966 A US 1482966A US 570749 A US570749 A US 570749A US 57074922 A US57074922 A US 57074922A US 1482966 A US1482966 A US 1482966A
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test apparatus
blood
tests
bacteriological test
bacteriological
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US570749A
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Llewellyn E W Bevan
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

Definitions

  • This invention relates to means for use in testing the blood of man or of the lower animale, with a view to determining the presence or otherwise of pathogenic bacteria.
  • samples of blood, drawn from suspected animals are sent to certain centres, where they are subjected to the "Widal reaction test or other tests; but in many instances the samples, in the course of transit to the testing station, undergo putrefaction, and arrive in a condition unsuitable for testing; in addition to which, where farms or other places of origin are widely scattered, much time is lost between the despatch of samples and the receipt of reports thereon.
  • My in vention is designed to avoid these objections by providing means whereby a farmer (for example) is himself enabled to apply to his cattle or sheep tests for the purpose of ascertaining whether they are affected by particu lar pathogenic bacteria, and thereupon to adopt proper remedies.
  • FIG. 1 of the accompanying drawings is represented, in vertical section, a device adapted for carrying my invention into effect;
  • Fig. 2 illustrating a modified method of construction, whilst
  • Fig. 3 shows another view of a detail pertaining to Fig. 2.
  • a is a glass vessel furnished with a ground-in glass stopper 7).
  • the lower eX- tremity of the stopper 6 is formed with a tubular extension 6 open at the bottom and having in its side a small air-vent-hole 5
  • the vessel a is charged with a diluted emulsion specially prepared with the'specific bac teria for which the test is required to be made. Blood is collected from the suspected animal, and the open extremity of the tubular extension applied thereto. By capillary attraction, blood rises in the tube, the air therein escapingby way of the vent-hole 6
  • the stopper b is then replaced in the vessel a and the contents well shaken.
  • the particular agglutinins for which the diluted emulsions is appropriate the specific bacteria agglutinate or cluster together, and finally sink to the bottom of the vessel; leaving the super-natent fluid transparent, so that the word Infect ed or other marking upon the inner side of the label, attached to the opposite side of the vessel, becomes legible. If, however, the blood tested be free from the particular agglutinins sought for by the test, the fluid remains cloudy, and the word Infected, or other marking upon the label, remains obsoured.
  • the ground-in stopper 6, with its tubular end 22 is dispensed with, and a cork closure substituted.
  • a cork composed of non-oxidizable metal, or other suitable filamentous material, which extends downwards and terminates in a small or close together to exert capillary attraction and to gather sufficient blood for carrying out the test operation hereinbefore described with reference to Fig. 1.
  • Fig. 3 shows the manner in which I prefer to form the looped end of the blood-collector, but this form is not essential.
  • Apparatus of the character described is particularly adapted for use in tests for detecting the presence of agglutinins caused by pathogenic bacteria in the blood of cattle, sheep and other farm animals, in consequence of the great advantage of being able to apply such tests on the spot, and forthwith to ascertain the results; but it is equally applicable for use in tests for tuberculosis, typhoid, Malta fever, and other maladies attributed to the presence of malignant bacteria in the blood either of man or of the lower animals.

Description

Feb. 5, 1924;
1.. E. w. B EVAN BACTERIOLOGICAL TEST APPARATUS Filed June 24. 1922 Fla-.5.
INVENTOR v a. Z. 6 fla Patented Feb 5, 1924.
UNITED STATES PATENT OFFICE.
LLEWELLYN E. W. BEVAN', OF SALISBURY, SOUTHERN RHODESIA, SOUTH AFRICA.
BAGTERIOLOGICAL TEST APPARATUS.
Application filedJune 24, 1922. Serial No. 570,749.
To all whom it may concern:
Be. it known that I, LLEWELLYN EDGAR WILLIAMS BnvAN, a subject of the King of Great Britain and Ireland, residing at the Veterinary Research Station, Salisbury, Southern Rhodesia, South Africa, have invented new and useful Improvements in Bacteriological Test Apparatus, of which the following is a specification.
This invention relates to means for use in testing the blood of man or of the lower animale, with a view to determining the presence or otherwise of pathogenic bacteria. According to the practice commonly prevailing, samples of blood, drawn from suspected animals, are sent to certain centres, where they are subjected to the "Widal reaction test or other tests; but in many instances the samples, in the course of transit to the testing station, undergo putrefaction, and arrive in a condition unsuitable for testing; in addition to which, where farms or other places of origin are widely scattered, much time is lost between the despatch of samples and the receipt of reports thereon. My in vention is designed to avoid these objections by providing means whereby a farmer (for example) is himself enabled to apply to his cattle or sheep tests for the purpose of ascertaining whether they are affected by particu lar pathogenic bacteria, and thereupon to adopt proper remedies.
In Fig. 1 of the accompanying drawings is represented, in vertical section, a device adapted for carrying my invention into effect; Fig. 2 illustrating a modified method of construction, whilst Fig. 3 shows another view of a detail pertaining to Fig. 2.
In Fig. 1, a is a glass vessel furnished with a ground-in glass stopper 7). The lower eX- tremity of the stopper 6 is formed with a tubular extension 6 open at the bottom and having in its side a small air-vent-hole 5 The vessel a is charged with a diluted emulsion specially prepared with the'specific bac teria for which the test is required to be made. Blood is collected from the suspected animal, and the open extremity of the tubular extension applied thereto. By capillary attraction, blood rises in the tube, the air therein escapingby way of the vent-hole 6 The stopper b is then replaced in the vessel a and the contents well shaken. If there be present in the blood the particular agglutinins for which the diluted emulsions is appropriate, the specific bacteria agglutinate or cluster together, and finally sink to the bottom of the vessel; leaving the super-natent fluid transparent, so that the word Infect ed or other marking upon the inner side of the label, attached to the opposite side of the vessel, becomes legible. If, however, the blood tested be free from the particular agglutinins sought for by the test, the fluid remains cloudy, and the word Infected, or other marking upon the label, remains obsoured.
According to the modification illustrated in Fig. 2, the ground-in stopper 6, with its tubular end 22 is dispensed with, and a cork closure substituted. In this cork is mounted a wire composed of non-oxidizable metal, or other suitable filamentous material, which extends downwards and terminates in a small or close together to exert capillary attraction and to gather sufficient blood for carrying out the test operation hereinbefore described with reference to Fig. 1. Fig. 3 shows the manner in which I prefer to form the looped end of the blood-collector, but this form is not essential.
Apparatus of the character described is particularly adapted for use in tests for detecting the presence of agglutinins caused by pathogenic bacteria in the blood of cattle, sheep and other farm animals, in consequence of the great advantage of being able to apply such tests on the spot, and forthwith to ascertain the results; but it is equally applicable for use in tests for tuberculosis, typhoid, Malta fever, and other maladies attributed to the presence of malignant bacteria in the blood either of man or of the lower animals.
I claim A device for use in bacteriological tests,
US570749A 1922-06-24 1922-06-24 Bacteriological test apparatus Expired - Lifetime US1482966A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3045494A (en) * 1958-03-13 1962-07-24 Gerarde Horace William Method of providing for blood count and pipette and assembly for use therein
US3185550A (en) * 1960-09-02 1965-05-25 Arthur H Thomas Company Apparatus for performing analyses
US3211531A (en) * 1963-01-21 1965-10-12 Theodor H Benzinger Miniaturized reaction vessel
WO2006056920A1 (en) * 2004-11-26 2006-06-01 Universite De Geneve Sample transfer system

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3045494A (en) * 1958-03-13 1962-07-24 Gerarde Horace William Method of providing for blood count and pipette and assembly for use therein
US3185550A (en) * 1960-09-02 1965-05-25 Arthur H Thomas Company Apparatus for performing analyses
US3211531A (en) * 1963-01-21 1965-10-12 Theodor H Benzinger Miniaturized reaction vessel
WO2006056920A1 (en) * 2004-11-26 2006-06-01 Universite De Geneve Sample transfer system

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