US11820993B2 - Peronospora resistance in Spinacia oleracea - Google Patents
Peronospora resistance in Spinacia oleracea Download PDFInfo
- Publication number
- US11820993B2 US11820993B2 US17/085,897 US202017085897A US11820993B2 US 11820993 B2 US11820993 B2 US 11820993B2 US 202017085897 A US202017085897 A US 202017085897A US 11820993 B2 US11820993 B2 US 11820993B2
- Authority
- US
- United States
- Prior art keywords
- pfs
- plant
- allele
- seq
- wolf
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000009337 Spinacia oleracea Nutrition 0.000 title claims description 44
- 244000300264 Spinacia oleracea Species 0.000 title claims description 14
- 241001223281 Peronospora Species 0.000 title description 6
- 241000219315 Spinacia Species 0.000 claims abstract description 164
- 108700028369 Alleles Proteins 0.000 claims abstract description 139
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 122
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 65
- 241000233679 Peronosporaceae Species 0.000 claims abstract description 49
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract description 32
- 241000936613 Peronospora farinosa Species 0.000 claims abstract description 23
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims abstract description 17
- 241000196324 Embryophyta Species 0.000 claims description 116
- 238000000034 method Methods 0.000 claims description 36
- 241000118319 Peronospora effusa Species 0.000 claims description 35
- 210000004027 cell Anatomy 0.000 claims description 22
- 239000000463 material Substances 0.000 claims description 11
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 239000002773 nucleotide Substances 0.000 claims description 9
- 238000003306 harvesting Methods 0.000 claims description 8
- 125000003729 nucleotide group Chemical group 0.000 claims description 8
- 210000001161 mammalian embryo Anatomy 0.000 claims description 6
- 230000002068 genetic effect Effects 0.000 claims description 4
- 230000000442 meristematic effect Effects 0.000 claims description 3
- 210000001672 ovary Anatomy 0.000 claims description 3
- 210000001938 protoplast Anatomy 0.000 claims description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 23
- 241000282461 Canis lupus Species 0.000 description 22
- 108020004707 nucleic acids Proteins 0.000 description 17
- 102000039446 nucleic acids Human genes 0.000 description 17
- 150000007523 nucleic acids Chemical group 0.000 description 17
- 230000001717 pathogenic effect Effects 0.000 description 17
- 238000012360 testing method Methods 0.000 description 12
- 108020004414 DNA Proteins 0.000 description 11
- 230000028070 sporulation Effects 0.000 description 10
- 108700026215 vpr Genes Proteins 0.000 description 10
- 208000015181 infectious disease Diseases 0.000 description 9
- 230000003321 amplification Effects 0.000 description 8
- 238000009395 breeding Methods 0.000 description 8
- 238000003199 nucleic acid amplification method Methods 0.000 description 8
- 244000052769 pathogen Species 0.000 description 8
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 8
- 230000001488 breeding effect Effects 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 208000024891 symptom Diseases 0.000 description 7
- 208000006278 hypochromic anemia Diseases 0.000 description 6
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 238000012163 sequencing technique Methods 0.000 description 5
- 108091093088 Amplicon Proteins 0.000 description 4
- 108091026890 Coding region Proteins 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 238000000137 annealing Methods 0.000 description 4
- 238000004925 denaturation Methods 0.000 description 4
- 230000036425 denaturation Effects 0.000 description 4
- 229910052697 platinum Inorganic materials 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 240000008415 Lactuca sativa Species 0.000 description 3
- 101150090155 R gene Proteins 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 229920006280 packaging film Polymers 0.000 description 3
- 239000012785 packaging film Substances 0.000 description 3
- 239000011543 agarose gel Substances 0.000 description 2
- 230000000875 corresponding effect Effects 0.000 description 2
- 230000007123 defense Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 230000007918 pathogenicity Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 235000012045 salad Nutrition 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 239000000304 virulence factor Substances 0.000 description 2
- 101100223980 Arabidopsis thaliana DMR6 gene Proteins 0.000 description 1
- 101100288015 Arabidopsis thaliana HSK gene Proteins 0.000 description 1
- 101150000533 CCM1 gene Proteins 0.000 description 1
- 241000283153 Cetacea Species 0.000 description 1
- 241000272201 Columbiformes Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 102100030569 Nuclear receptor corepressor 2 Human genes 0.000 description 1
- 101710153660 Nuclear receptor corepressor 2 Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000282320 Panthera leo Species 0.000 description 1
- 101100273578 Schizosaccharomyces japonicus (strain yFS275 / FY16936) dmr1 gene Proteins 0.000 description 1
- 101100273579 Schizosaccharomyces pombe (strain 972 / ATCC 24843) ppr3 gene Proteins 0.000 description 1
- 241001501942 Suricata suricatta Species 0.000 description 1
- 101000872823 Xenopus laevis Probable histone deacetylase 1-A Proteins 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000000680 avirulence Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 210000004901 leucine-rich repeat Anatomy 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000007481 next generation sequencing Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 244000000003 plant pathogen Species 0.000 description 1
- 238000007480 sanger sequencing Methods 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 230000009417 vegetative reproduction Effects 0.000 description 1
- 238000013466 vegetative reproduction Methods 0.000 description 1
- 238000012070 whole genome sequencing analysis Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
- A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- A01H1/1245—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance
- A01H1/1255—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance for fungal resistance
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
Definitions
- the invention relates to a gene capable of conferring resistance to a spinach plant against one or more Peronospora farinosa f. sp. spinaciae races.
- the invention also relates to a spinach plant, to propagation material of said spinach plant, to a cell of said spinach plant, and to seed of said spinach plant carrying the gene.
- the invention further relates to a method of producing a spinach plant carrying the gene and to the use of the gene in breeding to confer resistance against Peronospora farinosa f. sp. spinaciae.
- Races 4 to 16 were identified between 1990 and 2014, while only recently two new Peronospora isolates have been identified, termed UA201519B and US1602, which subsequently have been officially named Pfs:16 and Pfs:17 by the International Working Group on Peronospora (IWGP) (Plantum NL (Dutch association for breeding, tissue culture, production and trade of seed and young plants) press release, “Denomination of Pfs: 16, a new race of downy mildew in spinach”, Mar. 15, 2016; Plantum NL press release, Denomination of Pfs: 17, a new race of downy mildew in spinach”, Apr. 16, 2018.
- IWGP International Working Group on Peronospora
- the invention also relates to an alpha-WOLF 26 allele having an LRR domain which has a sequence that in order in order of increased preference has at least 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5%, 99%, 99.5%, 100% sequence identity to SEQ ID NO: 9.
- PCR conditions for amplifying the LRR domain-encoding region of an alpha-WOLF gene using primers having SEQ ID NO: 4 and SEQ ID NO: 5 are, using Platinum Taq enzyme (Thermo Fisher Scientific): 3 minutes at 95° C. (initial denaturing step); 40 amplification cycles, each cycle consisting of: 30 seconds denaturation at 95° C., 30 seconds annealing at 60° C., and 30 seconds extension at 72° C.; 2 minutes at 72° C. (final extension step).
- Determining the genomic DNA or coding DNA sequence of at least part of a WOLF gene in the genome of a spinach plant may be performed using any suitable molecular biological method known in the art, including but not limited to (genomic) PCR amplification followed by Sanger sequencing, whole-genome-sequencing, transcriptome sequencing, sequence-specific target capture followed by next-generation sequencing (using, for example, the xGen® target capture system of Integrated DNA Technologies), specific amplification of LRR-domain-which may comprise gene sequences (using, for example, the RenSeq methodology, as described in U.S. patent application Ser. No. 14/627,116, and in Jupe et al., 2013 , Plant J. 76: 530-544) followed by sequencing, etcetera.
- the invention relates to a spinach plant which may comprise an allele designated alpha-WOLF 26 which when present in a spinach plant homozygously confers complete resistance to at least Peronospora farinosa f. sp. spinacea race Pfs:7, Pfs:10, Pfs:11, Pfs:12, Pfs:13, Pfs:14, Pfs:15, Pfs:16, Pfs:17, and does not confer resistance to downy mildew races Pfs:8 and Pfs:9 or Pfs:1, Pfs:2, Pfs:3, Pfs:4, Pfs:5, Pfs:6, Pfs:7, Pfs:10, Pfs:11, Pfs:12, Pfs:13, Pfs:14, Pfs:15, Pfs:16, Pfs:17, and does not confer resistance to downy mildew races Pfs:8 and Pfs:9, wherein
- SEQ ID NO: 1 MAEIGYSVC
- SEQ ID NO: 2 KWMCLR
- SEQ ID NO: 3 HVGCVVDR
- SEQ ID NO: 4 ACAAGTGGATGTGTCTTAGG Forward primer LRR domain (Alpha)
- SEQ ID NO: 5 TTCGCCCTCATCTTCCTGG Reverse primer LRR domain
- SEQ ID NO: 6 TCACGTGGGTTGTGTTGT Forward primer LRR domain
- Beta) SEQ ID NO: 7: TCACGTGGGTTGTGTTGTCGATAGAGATCCAGAAATAGTCTTTA Amplicon of TGTAGCAATAAGATTCGTTCGTATATTAGCGGTCGCTGCATAAAG LRR domain of AATCCGGTGGATTCACAAATAGACAACTGGATGTGCCTTAGGGTG the beta-WOLF 0 TTGGACTTGTCAGATTCATGTGTTAAAGATTTGTCTGATTCAATAG allele (Viroflay) GTAAGCTGCTGCACTTAAGGTAT
- the DNA sequence of the LRR domain of the alpha-WOLF 26 allele amplified by primers having SEQ ID NO: 4 and SEQ ID NO: 5 is provided in Table 2 under SEQ ID NO: 9.
- PCR primers and PCR conditions were different.
- An agronomically elite spinach plant comprising an allele which when homozygously present in a spinach plant encodes a protein that confers complete resistance to at least Peronospora farinosa f. sp. spinaciae races Pfs:1, Pfs:2, Pfs:3, Pfs:4, Pfs:5, Pfs:6, Pfs:7, Pfs:10, Pfs:11, Pfs:12, Pfs:13, Pfs:14, Pfs:15, Pfs:16, Pfs:17, and does not confer resistance to downy mildew race Pfs:8 and Pfs:9, wherein the allele has a nucleotide sequence which has in order of increased preference at least 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5%, 99%, 99.5%, 100% sequence identity to SEQ ID NO: 12.
- the method includes performing the optional selection, and the selection of the plant comprising the allele expressing the protein comprises determining the presence of the allele according to a method comprising any or more of; determining the presence of a genomic nucleotide sequence in the genome of a plant, wherein said sequence in order of increased preference at least 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5%, 99%, 99.5%, 100% sequence identity to SEQ ID NO: 11, or determining the presence of a nucleotide sequence in a plant, wherein said sequence has in order of increased preference at least 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5%, 99%, 99.5%, 100% sequence identity to SEQ ID NO: 12, or determining the presence of a genomic nucleo
- a method of producing an F1 hybrid spinach seed comprising crossing a first parent spinach plant with a second parent spinach plant and harvesting the resultant hybrid spinach seed, wherein said first parent spinach plant and/or said second parent spinach plant is the agronomically elite spinach plant of any of the paragraphs 1 to 6.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17/085,897 US11820993B2 (en) | 2020-10-30 | 2020-10-30 | Peronospora resistance in Spinacia oleracea |
| AU2021368002A AU2021368002A1 (en) | 2020-10-30 | 2021-11-01 | Peronospora resistance in spinacia oleracea |
| PCT/EP2021/080279 WO2022090546A1 (en) | 2020-10-30 | 2021-11-01 | Peronospora resistance in spinacia oleracea |
| EP21799066.2A EP4236681A1 (en) | 2020-10-30 | 2021-11-01 | Peronospora resistance in spinacia oleracea |
| CN202180074089.4A CN116507200A (zh) | 2020-10-30 | 2021-11-01 | 菠菜中的霜霉病抗性 |
| US18/306,462 US20230383306A1 (en) | 2020-10-30 | 2023-04-25 | Peronospora resistance in spinacia oleracea |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17/085,897 US11820993B2 (en) | 2020-10-30 | 2020-10-30 | Peronospora resistance in Spinacia oleracea |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EPPCT/EP2020/080593 Continuation-In-Part | 2020-10-30 | 2020-10-30 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| US20220135996A1 US20220135996A1 (en) | 2022-05-05 |
| US11820993B2 true US11820993B2 (en) | 2023-11-21 |
Family
ID=81378826
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US17/085,897 Active US11820993B2 (en) | 2020-10-30 | 2020-10-30 | Peronospora resistance in Spinacia oleracea |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US11820993B2 (zh) |
| CN (1) | CN116507200A (zh) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4117426A1 (en) * | 2020-03-12 | 2023-01-18 | Rijk Zwaan Zaadteelt en Zaadhandel B.V. | Peronospora resistance in spinacia oleracea |
Citations (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050183150A1 (en) | 2004-02-13 | 2005-08-18 | Rebecca Torisky | Method for regenerating and transforming St. Augustinegrass from embryogenic callus |
| WO2007051483A1 (en) | 2005-11-01 | 2007-05-10 | Universiteit Utrecht Holding B.V. | Disease resistant plants |
| US20070204368A1 (en) | 2003-09-25 | 2007-08-30 | Dale James L | Banana Resistance Genes And Uses Thereof |
| US20090300786A1 (en) | 2008-05-30 | 2009-12-03 | Seminis Vegetable Seeds, Inc. | Spinach line omb66-1101m |
| US20090300788A1 (en) | 2008-05-30 | 2009-12-03 | Bernardus Baerends | Spinach line smb66-1082f |
| US20100031385A1 (en) | 2008-07-29 | 2010-02-04 | Seminis Vegetable Seeds, Inc. | Spinach line msa66-1119m |
| US20120054894A1 (en) | 2010-08-26 | 2012-03-01 | Jan Hugo Den Braber | Multileaf spinach |
| US20130055454A1 (en) | 2011-08-30 | 2013-02-28 | Jan Hugo Den Braber | Spinach hybrid 51-324 |
| US20130055422A1 (en) | 2011-08-26 | 2013-02-28 | Bernardus Baerends | Spinach hybrid rx 06681616 and parents thereof |
| WO2013064436A1 (en) | 2011-10-31 | 2013-05-10 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Peronospora resistance in spinacia oleracea |
| US20140068804A1 (en) | 2012-08-29 | 2014-03-06 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Hybrid spinach variety 51-707 rz |
| US20140068805A1 (en) | 2012-08-29 | 2014-03-06 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Hybrid spinach variety 51-332 rz |
| US20140068799A1 (en) | 2012-08-29 | 2014-03-06 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Hybrid spinach variety 51-336 rz |
| US20140068806A1 (en) | 2012-08-29 | 2014-03-06 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Hybrid spinach variety 51-333 rz |
| US20140068801A1 (en) | 2012-08-29 | 2014-03-06 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Hybrid spinach variety 51-331 rz |
| US20140065287A1 (en) | 2012-08-29 | 2014-03-06 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Hybrid spinach variety 51-335 rz |
| DE102013010026A1 (de) | 2013-06-17 | 2014-12-18 | Kws Saat Ag | Resistenzgen gegen Rizomania |
| EP2848114A1 (en) | 2013-09-13 | 2015-03-18 | Nunhems B.V. | Spinach plants that are resistant to downy mildew |
| WO2015036378A1 (en) | 2013-09-13 | 2015-03-19 | Nunhems B.V. | Spinach plants that are resistant to downy mildew |
| WO2015036469A1 (en) | 2013-09-13 | 2015-03-19 | Nunhems B.V. | Spinach plants that are resistant to downy mildew |
| US20150101073A1 (en) | 2013-10-08 | 2015-04-09 | Seminis Vegetable Seeds, Inc. | Methods and compositions for peronospora resistance in spinach |
| US20150240256A1 (en) | 2014-02-27 | 2015-08-27 | Seminis Vegetable Seeds, Inc. | Compositions and methods for peronospora resistance in spinach |
| US9121029B2 (en) | 2007-02-01 | 2015-09-01 | Enza Zaden Beheer B.V. | Disease resistant plants |
| WO2015171603A1 (en) | 2014-05-06 | 2015-11-12 | Two Blades Foundation | Methods for producing plants with enhanced resistance to oomycete pathogens |
| US9265276B2 (en) | 2008-09-25 | 2016-02-23 | Nestec S.A. | Reducing astringency in compositions containing phenolic compounds |
| US20160152099A1 (en) | 2013-07-18 | 2016-06-02 | Ankol Eood | Connector for transferring at least one fluid |
| US9402363B1 (en) | 2015-11-20 | 2016-08-02 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Peronospora resistance in spinacia oleracea |
| US20170027126A1 (en) | 2013-09-13 | 2017-02-02 | Nunhems B.V. | Spinach plants that are resistant to downy mildew |
| US20170027127A1 (en) | 2013-09-13 | 2017-02-02 | Nunhems B.V. | Spinach plants that are resistant to downy mildew |
| US20170127641A1 (en) | 2015-11-10 | 2017-05-11 | Pop Vriend Research B.V. | Peronospora resistance in spinacia sp |
| US20170127642A1 (en) | 2015-11-10 | 2017-05-11 | Pop Vriend Research B.V. | Peronospora resistance in spinacia sp |
| US20170327839A1 (en) | 2016-05-13 | 2017-11-16 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Non r-gene mediated resistance |
| WO2018060474A1 (en) | 2016-09-30 | 2018-04-05 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Method for modifying the resistance profile of spinacia oleracea to downy mildew |
| WO2018059653A1 (en) | 2016-09-30 | 2018-04-05 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Peronospora resistance in spinacia oleracea |
| US20190127753A1 (en) | 2017-09-29 | 2019-05-02 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Method for modifying the resistance profile of spinacia oleracea to downy mildew |
| US20200017875A1 (en) | 2016-09-30 | 2020-01-16 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Peronospora resistance in spinacia oleracea |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001031019A2 (en) * | 1999-10-29 | 2001-05-03 | Chiron Spa | Neisserial antigenic peptides |
-
2020
- 2020-10-30 US US17/085,897 patent/US11820993B2/en active Active
-
2021
- 2021-11-01 CN CN202180074089.4A patent/CN116507200A/zh active Pending
Patent Citations (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070204368A1 (en) | 2003-09-25 | 2007-08-30 | Dale James L | Banana Resistance Genes And Uses Thereof |
| US20050183150A1 (en) | 2004-02-13 | 2005-08-18 | Rebecca Torisky | Method for regenerating and transforming St. Augustinegrass from embryogenic callus |
| WO2007051483A1 (en) | 2005-11-01 | 2007-05-10 | Universiteit Utrecht Holding B.V. | Disease resistant plants |
| US9121029B2 (en) | 2007-02-01 | 2015-09-01 | Enza Zaden Beheer B.V. | Disease resistant plants |
| US20090300786A1 (en) | 2008-05-30 | 2009-12-03 | Seminis Vegetable Seeds, Inc. | Spinach line omb66-1101m |
| US20090300788A1 (en) | 2008-05-30 | 2009-12-03 | Bernardus Baerends | Spinach line smb66-1082f |
| US20100031385A1 (en) | 2008-07-29 | 2010-02-04 | Seminis Vegetable Seeds, Inc. | Spinach line msa66-1119m |
| US9265276B2 (en) | 2008-09-25 | 2016-02-23 | Nestec S.A. | Reducing astringency in compositions containing phenolic compounds |
| US20120054894A1 (en) | 2010-08-26 | 2012-03-01 | Jan Hugo Den Braber | Multileaf spinach |
| US20130055422A1 (en) | 2011-08-26 | 2013-02-28 | Bernardus Baerends | Spinach hybrid rx 06681616 and parents thereof |
| US20130055454A1 (en) | 2011-08-30 | 2013-02-28 | Jan Hugo Den Braber | Spinach hybrid 51-324 |
| WO2013064436A1 (en) | 2011-10-31 | 2013-05-10 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Peronospora resistance in spinacia oleracea |
| US20130230635A1 (en) | 2011-10-31 | 2013-09-05 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Peronospora resistance in spinacia oleracea |
| US20140068806A1 (en) | 2012-08-29 | 2014-03-06 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Hybrid spinach variety 51-333 rz |
| US20140068799A1 (en) | 2012-08-29 | 2014-03-06 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Hybrid spinach variety 51-336 rz |
| US20140068801A1 (en) | 2012-08-29 | 2014-03-06 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Hybrid spinach variety 51-331 rz |
| US20140065287A1 (en) | 2012-08-29 | 2014-03-06 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Hybrid spinach variety 51-335 rz |
| US20140068804A1 (en) | 2012-08-29 | 2014-03-06 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Hybrid spinach variety 51-707 rz |
| US20140068805A1 (en) | 2012-08-29 | 2014-03-06 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Hybrid spinach variety 51-332 rz |
| US10017781B2 (en) | 2013-06-17 | 2018-07-10 | Kws Saat Se | Rhizomania-resistant gene |
| DE102013010026A1 (de) | 2013-06-17 | 2014-12-18 | Kws Saat Ag | Resistenzgen gegen Rizomania |
| US20160152099A1 (en) | 2013-07-18 | 2016-06-02 | Ankol Eood | Connector for transferring at least one fluid |
| WO2015036469A1 (en) | 2013-09-13 | 2015-03-19 | Nunhems B.V. | Spinach plants that are resistant to downy mildew |
| US20150082483A1 (en) | 2013-09-13 | 2015-03-19 | Nunhems B.V. | Spinach plants that are resistant to downy mildew |
| WO2015036378A1 (en) | 2013-09-13 | 2015-03-19 | Nunhems B.V. | Spinach plants that are resistant to downy mildew |
| US20160177330A1 (en) | 2013-09-13 | 2016-06-23 | Nunhems B.V. | Spinach plants that are resistant to downy mildew |
| EP2848114A1 (en) | 2013-09-13 | 2015-03-18 | Nunhems B.V. | Spinach plants that are resistant to downy mildew |
| US20170027127A1 (en) | 2013-09-13 | 2017-02-02 | Nunhems B.V. | Spinach plants that are resistant to downy mildew |
| US20170027126A1 (en) | 2013-09-13 | 2017-02-02 | Nunhems B.V. | Spinach plants that are resistant to downy mildew |
| US20150101073A1 (en) | 2013-10-08 | 2015-04-09 | Seminis Vegetable Seeds, Inc. | Methods and compositions for peronospora resistance in spinach |
| US20150240256A1 (en) | 2014-02-27 | 2015-08-27 | Seminis Vegetable Seeds, Inc. | Compositions and methods for peronospora resistance in spinach |
| EP2912940A1 (en) | 2014-02-27 | 2015-09-02 | Seminis Vegetable Seeds, Inc. | Compositions and methods for peronospora resistance in spinach |
| WO2015171603A1 (en) | 2014-05-06 | 2015-11-12 | Two Blades Foundation | Methods for producing plants with enhanced resistance to oomycete pathogens |
| US20170127641A1 (en) | 2015-11-10 | 2017-05-11 | Pop Vriend Research B.V. | Peronospora resistance in spinacia sp |
| US20170127642A1 (en) | 2015-11-10 | 2017-05-11 | Pop Vriend Research B.V. | Peronospora resistance in spinacia sp |
| US20180042198A1 (en) | 2015-11-20 | 2018-02-15 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Peronospora resistance in spinacia oleracea |
| US10638688B2 (en) | 2015-11-20 | 2020-05-05 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Peronospora resistance in spinacia oleracea |
| US9402363B1 (en) | 2015-11-20 | 2016-08-02 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Peronospora resistance in spinacia oleracea |
| US9974276B2 (en) | 2015-11-20 | 2018-05-22 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Peronospora resistance in Spinacia oleracea |
| US20170327839A1 (en) | 2016-05-13 | 2017-11-16 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Non r-gene mediated resistance |
| WO2018059653A1 (en) | 2016-09-30 | 2018-04-05 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Peronospora resistance in spinacia oleracea |
| US20190233841A1 (en) | 2016-09-30 | 2019-08-01 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Peronospora resistance in spinacia oleracea |
| US20190241905A1 (en) | 2016-09-30 | 2019-08-08 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Method for modifying the resistance profile of spinacia oleracea to downy mildew |
| US20200017875A1 (en) | 2016-09-30 | 2020-01-16 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Peronospora resistance in spinacia oleracea |
| WO2018060474A1 (en) | 2016-09-30 | 2018-04-05 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Method for modifying the resistance profile of spinacia oleracea to downy mildew |
| US20190127753A1 (en) | 2017-09-29 | 2019-05-02 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Method for modifying the resistance profile of spinacia oleracea to downy mildew |
| US10633670B2 (en) * | 2017-09-29 | 2020-04-28 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Method for modifying the resistance profile of spinacia oleracea to downy mildew |
Non-Patent Citations (24)
| Title |
|---|
| 2011 APS-IPPC Joint Meeting Abstracts of Presentations, Phytopathology (2011) 101(6) Supplemental, S1, S52. |
| Adam Bentham, et al., Animal NLRs Provide Structural insights into Plant NLF Function, Annals of Botany (2017) 119:889-702. |
| B. M. Irish, et al., Characterization of a Resistance Locus (Pfs-1) to the Spinach Downy Mildew Pathogen (Peronospora farinosa f. sp. spinaciae) and Development of a Molecular Marker Linked to Pfs-1, Pathology, American Phytopathological Society, US (2008) vol. 98, No. 8, p. 894-900. |
| B. M. Irish, et al., Three New Races of the Spinach Downy Mildew Pathogen Identified by a Modified Set of Spinach Differentials, Plant Disease (Nov. 2007) vol. 91, No. 11, p. 1392-1396. |
| Charlotte Hallavant, et al., The First Archaeobotanical Evidence of Spinacia oleracea L. (Spinach) in Late 12th-mid 13th Century A.D. France, French National Centre for Scientific Research, Article: Vegetation History and Archaeobotany, Published online May 21, 2013. |
| Dong Qi, et al., Recent Advances in Plant NLR Structure, Function, Localization, and Signaling, Frontiers in Immunology (2013) vol. 4, Article 348, p. 1-10. |
| Feng Chunda, et al., Construction of a Spinach Bacterial Artificial Chromosome (BAC) Library as a Resource for Gene Identification and Marker Development, Plant Mol Biol Rep (2015) 33:1996-2005. |
| Feng, et al., Identification of New Races and Deviating Strains of the Spinach Downy Mildew Pathogen Peronospora farinosa f, sp. spinaciae, Plant Disease (Jan. 2014) 98(1):145-152. |
| GenBank Accession No. XP_021842255 (Aug. 1, 2017). |
| Haiwei H. Guo, et al., Protein Tolerance to Random Amino Acid Change, PNAS (Jun. 22. 2004) vol. 101, No. 25, p. 9205-9210. |
| Hongbing She, et al., Fine Mapping and Candidate Gene Screening of the Downy Mildew Resistance Gene RPF1 in Spinach, Theoretical and Applied Genetics (2018) 131:2529-2541. |
| International Search Report and Written Opinion dated Jan. 30. 2018 in PCT/EP2017/074863. |
| J.C. Correll, et al., Spinach: Better Management of Downy Mildew and White Rust Through Genomics. Eur. J. Plant Pathology (Dec. 4, 2010) 129:193-205. |
| Joydeep Chakraborty, et al., Functional Diversification of Structurally Alike NLR Proteins in Plants. Plant Science (2018) 269:85-93. |
| Leah McHale, et al., Plant NBSMLRR proteins: adaptable guards. Genome Biology (2006) vol. 7, Issue 4, Article 212, p. 212-212.11. |
| Merriam Webster Definition of "as" Sep. 27, 2016. |
| Moffett et al., Interaction Between Domain of a Plant NBS-LRR Protein in Disease Resistance-Related Cell Death, The EMBO Journal (2002) vol. 21. No. 17, p. 4511-4519. |
| Octavina C.A. Sukarta, et al., Structure-Informed Insights for NLR Functioning in Plant Immunity, Seminars in Cell & Developmental Biology (2016) 56:134-149. |
| Peter N. Dodds, et al., Six Amino Acid Changes Confined to the Leucine-Rich Repeat β-Strand/β-Turn Motif Determine the Difference between the P and P2 Rust Resistance Specificities in Flax, The Plant Cell (Jan. 2001) vol. 13. p. 163-178. |
| Peter N. Dodds. et al., Direct protein interaction underlies gene-for-gene specificity and coevolution of the flax resistance genes and flax rust avirulence genes , PNAS (Jun. 6, 2006) vol. 103, No. 23, p. 8888-8893. |
| Simona Proietti, et al., Increase of Ascorbic Acid Content and Nutritional Quality in Spinach Leaves During Physiological Acclimation to Low Temperature, Plant Physiology and Biochemistry (2009) vol. 47, p. 717-723. |
| Timothy K. Eitas, et al., NB-LRR Proteins: Pairs, Pieces, Perception, Partners, and Pathways, Current Opinion in Plant Biology (2010) 13:472-477. |
| Xiaoping Gou, et al., Genome-wide cloning and sequence analysts of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana, BMC Genomics (2010) vol. 11, No. 19, p. 1-15. |
| Yanming Yang, et al., Transgenic Spinach Plants Expressing the Coat Protein of Cucumber Mosaic Virus, In Vitro Cell Dev. Biol.-Plant(1997) 33:200-204. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116507200A (zh) | 2023-07-28 |
| US20220135996A1 (en) | 2022-05-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10638688B2 (en) | Peronospora resistance in spinacia oleracea | |
| US11202421B2 (en) | Peronospora resistance in spinacia oleracea | |
| US11203766B2 (en) | Peronospora resistance in spinacia oleracea | |
| US11603538B2 (en) | Peronospora resistance in Spinacia oleracea | |
| US11820993B2 (en) | Peronospora resistance in Spinacia oleracea | |
| US20230257763A1 (en) | Peronospora resistance in spinacia oleracea | |
| US11473102B2 (en) | Peronospora resistance in Spinacia oleracea | |
| US20230383306A1 (en) | Peronospora resistance in spinacia oleracea | |
| US20230329169A1 (en) | Peronospora resistance in spinacia oleracea | |
| US10674688B2 (en) | Peronospora resistance in spinacia oleracea | |
| WO2022090546A1 (en) | Peronospora resistance in spinacia oleracea | |
| WO2022090543A1 (en) | Peronospora resistance in spinacia oleracea | |
| WO2023208632A1 (en) | Peronospora resistance in spinacia oleracea | |
| US20210403937A1 (en) | Cmv resistance allele |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FEPP | Fee payment procedure |
Free format text: ENTITY STATUS SET TO UNDISCOUNTED (ORIGINAL EVENT CODE: BIG.); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY |
|
| AS | Assignment |
Owner name: RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V., NETHERLANDS Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:DEN BOER, ERIK;FRIJTERS, RAOUL JACOBUS JOHANNES MARIA;SIGNING DATES FROM 20201111 TO 20201117;REEL/FRAME:054541/0887 |
|
| STPP | Information on status: patent application and granting procedure in general |
Free format text: NOTICE OF ALLOWANCE MAILED -- APPLICATION RECEIVED IN OFFICE OF PUBLICATIONS |
|
| STPP | Information on status: patent application and granting procedure in general |
Free format text: AWAITING TC RESP., ISSUE FEE NOT PAID |
|
| STPP | Information on status: patent application and granting procedure in general |
Free format text: NOTICE OF ALLOWANCE MAILED -- APPLICATION RECEIVED IN OFFICE OF PUBLICATIONS |
|
| STPP | Information on status: patent application and granting procedure in general |
Free format text: DOCKETED NEW CASE - READY FOR EXAMINATION |
|
| STPP | Information on status: patent application and granting procedure in general |
Free format text: NON FINAL ACTION MAILED |
|
| STPP | Information on status: patent application and granting procedure in general |
Free format text: RESPONSE TO NON-FINAL OFFICE ACTION ENTERED AND FORWARDED TO EXAMINER |
|
| STPP | Information on status: patent application and granting procedure in general |
Free format text: NOTICE OF ALLOWANCE MAILED -- APPLICATION RECEIVED IN OFFICE OF PUBLICATIONS |
|
| STPP | Information on status: patent application and granting procedure in general |
Free format text: PUBLICATIONS -- ISSUE FEE PAYMENT VERIFIED |
|
| STCF | Information on status: patent grant |
Free format text: PATENTED CASE |