US11800871B2 - Paenibacillus elgii AM-67 strain and biopesticide composition containing same - Google Patents

Paenibacillus elgii AM-67 strain and biopesticide composition containing same Download PDF

Info

Publication number
US11800871B2
US11800871B2 US16/789,942 US202016789942A US11800871B2 US 11800871 B2 US11800871 B2 US 11800871B2 US 202016789942 A US202016789942 A US 202016789942A US 11800871 B2 US11800871 B2 US 11800871B2
Authority
US
United States
Prior art keywords
strain
chinese cabbage
rot disease
present disclosure
soft rot
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active, expires
Application number
US16/789,942
Other versions
US20200187508A1 (en
Inventor
Gi Seok KWON
Jung Bok Lee
Dan Bi Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Andong National University Industry Academic Cooperation Foundation
Original Assignee
Andong National University Industry Academic Cooperation Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/KR2018/001253 external-priority patent/WO2019142963A1/en
Application filed by Andong National University Industry Academic Cooperation Foundation filed Critical Andong National University Industry Academic Cooperation Foundation
Assigned to ANDONG NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION reassignment ANDONG NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: KWON, GI SEOK, LEE, DAN BI, LEE, JUNG BOK
Publication of US20200187508A1 publication Critical patent/US20200187508A1/en
Application granted granted Critical
Publication of US11800871B2 publication Critical patent/US11800871B2/en
Active legal-status Critical Current
Adjusted expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/25—Paenibacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Definitions

  • the present disclosure relates to novel Paenibacilius elgii AM-67 strain having antibacterial activity on phytobacterial pathogens causing Chinese cabbage soft rot disease, and an environmentally friendly biopesticide containing the same.
  • Chinese cabbage Brassica campestris subsp. napus var pekinensis
  • kimchi is a biennial plant belonging to Cruciferae, which is used as the main ingredient of kimchi, and is one of the highly produced and consumed vegetables in Korea.
  • kimchi has been recognized for its food and nutritional value, and thus is growing in demand not only in the Korean domestic market but also in the world market.
  • Chinese cabbage is a cryophilic vegetable that likes the cool climate; and in a case where Chinese cabbage grows above 20° C. that is an optimum growth temperature, the Chinese cabbage is affected in terms of head formation and appearance of bacteria ( Erwinia carotovora subsp.
  • carotovora is a bacterial disease that causes damage to various vegetables, including Chinese cabbage, radish, potato, carrot, and the like.
  • the Chinese cabbage soft rot disease is a bacterial soil disease that has a very low chemical controlling effect, and thus prophylactic application must be done.
  • Various methods are used to control bacterial soft rot diseases.
  • chemical controlling is mainly used due to the very high cost of soil fumigation.
  • chemical pesticides kill other useful soil microorganisms as well; and use of agricultural antibiotics results in emergence of resistant bacteria, thereby causing a variety of problems.
  • Biopesticides which are environmentally friendly controlling agents, do not cause these problems, and have safety and little toxicity to human and livestock.
  • the biopesticides utilize microorganisms widely present in nature, and thus may minimize problems of ecosystem disturbance.
  • Recently, development of environmentally friendly microbial pesticides has been spurred, and many attempts have been made.
  • the result is merely a composition using a culture solution.
  • novel Bacillus sp. microorganism having effect of protection on Chinese cabbage bacterial soft-rot disease Korean Patent No. 580336
  • novel Streptomyces sp. strain with quorum sensing inhibition activity Korean Patent No.
  • Another aspect is a method including designating a strain, which is obtained through total genomic analysis and consists of a total chromosome length of 8,072,494 bp, as Paenibacillus elgii AM-67, and depositing the strain with Korea Research institute of Bioscience and Biotechnology on Jan. 23, 2018 under Accession No. KCTC 13466BP; and a step of identifying, with a culture composition obtained from culture using the isolated strain of the present disclosure and a crude extract thereof, its antibacterial activity on Erwinia bacteria causing Chinese cabbage soft rot disease, and evaluating its use as an environmentally friendly biological material.
  • Another aspect is a controlling method in which soil or plants are treated with controlling agents for Chinese cabbage soft rot disease and similar soft rot diseases through various formulation schemes using the strain and its culture solution or an extract thereof.
  • At least one of the disclosed embodiments provides an excellent effect from the viewpoint of providing novel Paenibacillus elgii AM-67 strain that controls Chinese cabbage soft rot disease, and a hiopesticide containing the same.
  • FIG. 1 illustrates a diagram showing taxonomic classification obtained by 16s rRNA sequencing for identification of the novel microbial strain of the present disclosure.
  • FIG. 2 illustrates a genetic map of the novel microbial strain of the present disclosure.
  • FIG. 4 illustrates pictures showing antibacterial control activity of microbial strain of the present disclosure using the disk diffusion method.
  • the present disclosure relates to biological control against Erwinia sp. causing a bacterial rot disease that is Chinese cabbage blight, using the isolated strain Paenibacillus elgii AM-67 (Accession No. KCTC 13466BP).
  • the gene of the newly isolated strain of the present disclosure is related to a novel microbial Paenibacillus elgii AM-67 strain, having a chromosomal nucleotide sequence consisting of a total length of 8,042,494 bp, and a biopesticide containing the same controls soil microorganism-induced Chinese cabbage soft rot disease caused by infection through the roots during a growth period for Chinese cabbage cultivation, so that the Chinese cabbage soft rot disease is effectively controlled in a case where the biopesticide is applied on the Chinese cabbage field.
  • a biopesticide containing a culture composition which contains the Paenibacillus elgii AM-67 strain, and/or an extract thereof provides an environmentally friendly biopesticide material that enables environmentally friendly cultivation.
  • Some embodiments of the present disclosure provide Paenibacillus elgii ANI-67 (KCTC 13466BP), its culture composition, and a crude extract thereof, which control Chinese cabbage soft rot disease.
  • KCTC 13466BP Paenibacillus elgii ANI-67
  • inventions of the present disclosure provide a basis for control activity of a culture composition on Erwinia causing Chinese cabbage soft rot disease, the culture composition containing, as an active ingredient, the microorganism of the present disclosure, its culture solution, or a crude extract thereof.
  • soil samples were suspended and diluted in sterile physiological saline, inoculated in LB agar medium, and cultured at 30° C. for 2 days, so that isolation of microorganisms was performed.
  • Table 2 shows CDS, tRNA, and rRNA which have been completed from sequencing based on genomic data analysis for the strain of the present disclosure, and the final completed genetic map is illustrated in FIG. 2 .
  • the microbial Paenibacillus elgii AM-67 strain of the present disclosure was cultured at 30° C. for 3 days in a medium supplemented with 3% glucose, 3% yeast extract, and 5% NaCl. Then, the culture supernatant was taken, and antibacterial control activity thereof was identified using the disk diffusion method generally known for Erwinia causing Chinese cabbage soft rot disease. 100 ⁇ L of the culture supernatant of Paenibacillus elgii AM-67 strain of the present disclosure was loaded on each test disk and drying was performed. Then, the disk was placed on a medium smeared with Erwinia causing Chinese cabbage soft rot disease, and examined for antibacterial activity level thereof.

Abstract

The present disclosure relates to a novel strain for inhibiting a Chinese cabbage soft rot disease from microorganisms isolated from samples of a bacillus source of soil polluted with a soft-rot disease, a culture medium and crude extract using the culture medium, an environmentally friendly plant disease control agent using same, and a method for utilizing same as an environment-friendly preparation biopesticide by using same as a raw material. The present disclosure also relates to a control method for treating soils or plants using an environmentally friendly Chinese cabbage soft-rot disease control agent through various formulation methods by using the strain, a culture medium thereof, and a crude extract thereof.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation application, and claims the benefit under 35 U.S.C. § 120 and § 365 of PCT Application No. PCT/KR2018/001253, filed on Jan. 29, 2018, which is hereby incorporated by reference. PCT/KR2018/001253 also claimed priority to Korean Patent Application No. 10-2018-0007953 filed on Jan. 22, 2018 and Korean Patent Application No. 10-2018-0010792 filed on Jan. 29, 2018, both of which are hereby incorporated by reference.
SEQUENCE LISTING IN ELECTRONIC FORMAT
The present application is being filed along with an Electronic Sequence Listing as an ASCII text tile via EFS-Web. The Electronic Sequence Listing is provided as a file entitled NAM011002C1_SEQLIST.txt, created and last saved on Feb. 12, 2020, which is 2,492 bytes in size. The information in the Electronic Sequence Listing is incorporated herein by reference in its entirety.
BACKGROUND Technical Field
The present disclosure relates to novel Paenibacilius elgii AM-67 strain having antibacterial activity on phytobacterial pathogens causing Chinese cabbage soft rot disease, and an environmentally friendly biopesticide containing the same.
Related Technology
Chinese cabbage (Brassica campestris subsp. napus var pekinensis) is a biennial plant belonging to Cruciferae, which is used as the main ingredient of kimchi, and is one of the highly produced and consumed vegetables in Korea. Along with the well-being boom in recent years, kimchi has been recognized for its food and nutritional value, and thus is growing in demand not only in the Korean domestic market but also in the world market. Chinese cabbage is a cryophilic vegetable that likes the cool climate; and in a case where Chinese cabbage grows above 20° C. that is an optimum growth temperature, the Chinese cabbage is affected in terms of head formation and appearance of bacteria (Erwinia carotovora subsp. carotovora) causing a soft rot disease results in decreased production and deteriorated quality (Opena et al., 1988; De Boer and Kelman, 1978 Potato Res.; Stommel et al., 1996, Plant Dis.; Eun-Kyoung Chung, 2003 The Korean Journal of Pesticide Science; Hee-Young Kwon et al., 2009 Journal of Life Science; Anupama et al., 2009, The Korean Journal of Pesticide Science; Korean Patent No. 580336 owned by Chunghuk National University Industry Academy Cooperation Foundation). The Chinese cabbage soft rot disease is a plant infectious disease caused by Erwinia carotovora subsp. carotovora; and such a disease is a bacterial disease that causes damage to various vegetables, including Chinese cabbage, radish, potato, carrot, and the like. The Chinese cabbage soft rot disease is a bacterial soil disease that has a very low chemical controlling effect, and thus prophylactic application must be done. Various methods are used to control bacterial soft rot diseases. Among these, chemical controlling is mainly used due to the very high cost of soil fumigation. However, in a case where chemical pesticides are used, such chemical pesticides kill other useful soil microorganisms as well; and use of agricultural antibiotics results in emergence of resistant bacteria, thereby causing a variety of problems.
Biopesticides, which are environmentally friendly controlling agents, do not cause these problems, and have safety and little toxicity to human and livestock. In general, the biopesticides utilize microorganisms widely present in nature, and thus may minimize problems of ecosystem disturbance. Recently, development of environmentally friendly microbial pesticides has been spurred, and many attempts have been made. However, the result is merely a composition using a culture solution. Currently, regarding microorganisms for controlling soft rot diseases, the following subject matters have been reported: novel Bacillus sp. microorganism having effect of protection on Chinese cabbage bacterial soft-rot disease (Korean Patent No. 580336); novel Streptomyces sp. strain with quorum sensing inhibition activity (Korean Patent No. 1206883); Piericidin A1 with competitive inhibitory activity of acyl homoserine lactone (Korean Patent No. 743673); in a case where an enzyme (lactonase) that degrades homoserine lactone, a quorum sensing signal substance, in phytopathogenic bacteria, is isolated from microorganisms, and is transformed into potato and tobacco, resistance is induced against a soft rot disease caused by pathogenic bacteria in which pathogenic factors are induced by quorum sensing signals (Korean Patent Application No. 2003-7002587); and novel strains of Lactobacillus KLFOI and Lactococcus KLC02 and compositions for controlling plant disease using same (Korean Patent No. 1010537).
As described above, up to now, most studies have been conducted on inhibition of Chinese cabbage soft rot disease through control of quorum, and transformation of Chinese cabbage using Bacillus, Lactobacillus, and genetic modification.
SUMMARY OF CERTAIN INVENTIVE ASPECTS
One inventive aspect is to isolate a novel microbial strain that controls Erwinia sp. bacteria causing Chinese cabbage soft rot disease, and to provide a biopesticide containing the same.
Another aspect is a method including designating a strain, which is obtained through total genomic analysis and consists of a total chromosome length of 8,072,494 bp, as Paenibacillus elgii AM-67, and depositing the strain with Korea Research institute of Bioscience and Biotechnology on Jan. 23, 2018 under Accession No. KCTC 13466BP; and a step of identifying, with a culture composition obtained from culture using the isolated strain of the present disclosure and a crude extract thereof, its antibacterial activity on Erwinia bacteria causing Chinese cabbage soft rot disease, and evaluating its use as an environmentally friendly biological material.
Another aspect is a controlling method in which soil or plants are treated with controlling agents for Chinese cabbage soft rot disease and similar soft rot diseases through various formulation schemes using the strain and its culture solution or an extract thereof.
At least one of the disclosed embodiments provides an excellent effect from the viewpoint of providing novel Paenibacillus elgii AM-67 strain that controls Chinese cabbage soft rot disease, and a hiopesticide containing the same.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 illustrates a diagram showing taxonomic classification obtained by 16s rRNA sequencing for identification of the novel microbial strain of the present disclosure.
FIG. 2 illustrates a genetic map of the novel microbial strain of the present disclosure.
FIG. 3 illustrates pictures showing antibacterial activity of the novel microbial strain of the present disclosure on Chinese cabbage soft rot disease.
FIG. 4 illustrates pictures showing antibacterial control activity of microbial strain of the present disclosure using the disk diffusion method.
 DETAILED DESCRIPTION OF CERTAIN INVENTIVE EMBODIMENTS
The present disclosure relates to biological control against Erwinia sp. causing a bacterial rot disease that is Chinese cabbage blight, using the isolated strain Paenibacillus elgii AM-67 (Accession No. KCTC 13466BP). The gene of the newly isolated strain of the present disclosure is related to a novel microbial Paenibacillus elgii AM-67 strain, having a chromosomal nucleotide sequence consisting of a total length of 8,042,494 bp, and a biopesticide containing the same controls soil microorganism-induced Chinese cabbage soft rot disease caused by infection through the roots during a growth period for Chinese cabbage cultivation, so that the Chinese cabbage soft rot disease is effectively controlled in a case where the biopesticide is applied on the Chinese cabbage field. Thus, a biopesticide containing a culture composition, which contains the Paenibacillus elgii AM-67 strain, and/or an extract thereof provides an environmentally friendly biopesticide material that enables environmentally friendly cultivation.
Some embodiments of the present disclosure provide Paenibacillus elgii ANI-67 (KCTC 13466BP), its culture composition, and a crude extract thereof, which control Chinese cabbage soft rot disease.
Other embodiments of the present disclosure provide a basis for control activity of a culture composition on Erwinia causing Chinese cabbage soft rot disease, the culture composition containing, as an active ingredient, the microorganism of the present disclosure, its culture solution, or a crude extract thereof.
Hereinafter, embodiments of the present disclosure will be described in detail by way of examples.
EXAMPLE 1 Isolation and Selection of Bacteria Showing Antibacterial Activity on Erwinia Causing Chinese Cabbage Soft Rot Disease
To isolate microorganisms showing antibacterial activity on Erwinia causing Chinese cabbage soft rot disease, soil samples were suspended and diluted in sterile physiological saline, inoculated in LB agar medium, and cultured at 30° C. for 2 days, so that isolation of microorganisms was performed.
Among the purely isolated strains, to isolate and select strains having antibacterial activity on Chinese cabbage soft rot disease, culture supernatants of the respective strains were used to select strains showing antibacterial capacity.
16S rRNA sequencing was performed for taxonomic identification of the bacteria having antibacterial activity, and the strain having excellent final antibacterial activity was deposited with Korea Research Institute of Bioscience and Biotechnology, designated as a depositary institution for microorganisms under the Budapest Treaty, on Jan. 23, 2018 under Accession No. KCTC 13466BP (FIG. 1 )
EXAMPLE 2 Genetic Analysis of Antagonistic Bacteria of Present Disclosure
For genetic analysis of antagonistic bacteria of the present invention, such analysis was performed through whole genome de novo sequencing. A request for the analysis was made to Macrogen Inc. Consensus sequences as shown in Table 1 were obtained by the de novo assembly approach (see SEQ ID NO: 1).
TABLE 1
Summary of assembly
Contig Total Max Min Avg
Name Length N50 Length Length Length
contig
1 8,042,494 8,042,494 8,042,494 8,042,494 8,042,494
[Note]
Number of Contigs: The number of contigs assembled.
Total Length: The total length of contigs.
N50: 50% of all bases come from contigs longer than this value.
Max Length: The length of maximum contig.
Mm Length: The length of minimum contig.
Avg Length: The average length of contigs assembled.
EXAMPLE 3 Performing of Genome Annotation of Microbial Strain of Present Disclosure
For genetic analysis of the antagonistic bacteria of the present disclosure, genome annotation, which had been completed through whole genome de novo sequencing, was performed. Table 2 shows CDS, tRNA, and rRNA which have been completed from sequencing based on genomic data analysis for the strain of the present disclosure, and the final completed genetic map is illustrated in FIG. 2 .
TABLE 2
7,369 CDS, 96 tRNA, 30 rRNA genes were
discovered in present disclosure
Contig Name Length (bp) CDS tRNA rRNA
contig 1 8,042,494 7,369 96 30
Total 8,042,494 7,369 96 30
[Note]
Length (bp): The number of bases in each contig
CDS: Coding Sequence
tRNA: Transfer RNA, tRNA has triplet nucleotide sequence complementary to the triplet nucleotide coding sequences of messenger RNA (mRNA)
rRNA: Ribosomal RNA, a molecular component of ribosome
EXAMPLE 4 Measurement of Culture Conditions for Microbial Strain of Present Disclosure and Antibacterial Activity Thereof
Culture conditions that allow the antagonistic bacteria of the present disclosure to have control capacity on Erwinia causing Chinese cabbage soft rot disease, and antibacterial activity of the antagonistic bacteria were measured.
The microbial Paenibacillus elgii AM-67 strain of the present disclosure was cultured at 30° C. for 3 days in a medium supplemented with 3% glucose, 3% yeast extract, and 5% NaCl. Then, the culture supernatant was taken, and antibacterial control activity thereof was identified using the disk diffusion method generally known for Erwinia causing Chinese cabbage soft rot disease. 100 μL of the culture supernatant of Paenibacillus elgii AM-67 strain of the present disclosure was loaded on each test disk and drying was performed. Then, the disk was placed on a medium smeared with Erwinia causing Chinese cabbage soft rot disease, and examined for antibacterial activity level thereof.
As a result of the experiments, it was examined that the strain shows antibacterial activity on Erwinia causing Chinese cabbage soft rot disease (FIG. 3 ).
EXAMPLE 5 Control Activity for Inhibition of Soft Rot Disease in Case of Using Solvent Extract of Culture Supernatant of Bacillus sp. AM-67 Strain of Present Disclosure
Experiments were carried out to identify inhibition capacity, on Chinese cabbage soft rot disease, of an organic solvent crude extract of the culture supernatant of the antagonistic bacterial Paenibacillus elgii AM-67 strain of the present disclosure. The culture supernatant was extracted stepwise with hexane, chloroform, ethyl acetate, and butanol, to obtain a crude extract, and antibacterial control activity thereof was identified by using the disk diffusion method generally known for Erwinia causing Chinese cabbage soft rot disease. The experimental results are illustrated in FIG. 4 .

Claims (2)

What is claimed is:
1. A biopesticide composition for Chinese cabbage soft rot disease caused by Erwinia sp., comprising, as an active ingredient, an extract including:
chloroform;
a supernatant of a culture solution comprising Paenibacillus elgii AM-67 strain (KCTC 13466BP), 3% glucose, 3% yeast extract, and 5% NaCl.
2. The biopesticide composition of claim 1, wherein the Paenibacillus elgii AM-67 strain comprises a nucleotide sequence of SEQ ID NO: 1.
US16/789,942 2018-01-22 2020-02-13 Paenibacillus elgii AM-67 strain and biopesticide composition containing same Active 2038-09-22 US11800871B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
KR20180007953 2018-01-22
KR10-2018-0007953 2018-01-22
KR10-2018-0010792 2018-01-29
KR1020180010792A KR102012583B1 (en) 2018-01-22 2018-01-29 Novel Paenibacillus elgii AM-67 and echo-freindly agro-chemicals for controlling soft-root disease of chinese cabage comprising of the same
PCT/KR2018/001253 WO2019142963A1 (en) 2018-01-22 2018-01-29 Novel paenibacillus elgii am-67 strain and environmentally friendly biopesticide composition containing same for controlling chinese cabbage soft rot disease, erwinia.sp.

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/001253 Continuation WO2019142963A1 (en) 2018-01-22 2018-01-29 Novel paenibacillus elgii am-67 strain and environmentally friendly biopesticide composition containing same for controlling chinese cabbage soft rot disease, erwinia.sp.

Publications (2)

Publication Number Publication Date
US20200187508A1 US20200187508A1 (en) 2020-06-18
US11800871B2 true US11800871B2 (en) 2023-10-31

Family

ID=67615634

Family Applications (1)

Application Number Title Priority Date Filing Date
US16/789,942 Active 2038-09-22 US11800871B2 (en) 2018-01-22 2020-02-13 Paenibacillus elgii AM-67 strain and biopesticide composition containing same

Country Status (2)

Country Link
US (1) US11800871B2 (en)
KR (1) KR102012583B1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111172065B (en) * 2020-01-06 2022-06-24 河南新仰韶生物科技有限公司 High-yield paenibacillus polymyxa and application thereof

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030065468A (en) 2000-08-23 2003-08-06 인스티튜트 오브 몰레큘라 애그로바이올로지 Bacterial strains, genes and enzymes for control of bacterial diseases by quenching quorum-sensing signals
KR100580336B1 (en) 2003-12-15 2006-05-15 충북대학교 산학협력단 Novel Bacillus sp. Having a Effect of Protection of Bacterial Soft-rot Disease of Chinese cabbage
KR100743672B1 (en) 2006-04-05 2007-07-30 한국생명공학연구원 Piericidin a1 with competitive inhibitory activity of acyl homoserine lactone
KR101010537B1 (en) 2010-03-24 2011-01-24 주식회사 누림 Novel strains of lactobacillus fermentum klf01 and lactococcus lactis klc02 having effect on suppression of bacterial soft rot disease and composition for controlling plant disease
KR101206883B1 (en) 2010-03-11 2012-12-04 한국생명공학연구원 Novel Streptomyces sp. with Quorum Sensing inhibition activity
KR20150101702A (en) 2014-02-27 2015-09-04 안동과학대학교 산학협력단 Composition for having effect on suppression of bacterial soft rot disease and control method for the same
KR20160041084A (en) 2014-10-06 2016-04-18 재단법인 전남생물산업진흥원 Paenibacillus elgii 34-6 with antifungal activity to plant disease
US20160278388A1 (en) 2015-03-26 2016-09-29 Bayer Cropscience Lp Novel paenibacillus strain, antifungal compounds, and methods for their use

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030065468A (en) 2000-08-23 2003-08-06 인스티튜트 오브 몰레큘라 애그로바이올로지 Bacterial strains, genes and enzymes for control of bacterial diseases by quenching quorum-sensing signals
US20080182790A1 (en) 2000-08-23 2008-07-31 Agency For Science, Technology And Research Bacterial strains, genes and enzymes for control of bacterial diseases by quenching quorum-sensing signals
KR100580336B1 (en) 2003-12-15 2006-05-15 충북대학교 산학협력단 Novel Bacillus sp. Having a Effect of Protection of Bacterial Soft-rot Disease of Chinese cabbage
KR100743672B1 (en) 2006-04-05 2007-07-30 한국생명공학연구원 Piericidin a1 with competitive inhibitory activity of acyl homoserine lactone
KR101206883B1 (en) 2010-03-11 2012-12-04 한국생명공학연구원 Novel Streptomyces sp. with Quorum Sensing inhibition activity
KR101010537B1 (en) 2010-03-24 2011-01-24 주식회사 누림 Novel strains of lactobacillus fermentum klf01 and lactococcus lactis klc02 having effect on suppression of bacterial soft rot disease and composition for controlling plant disease
KR20150101702A (en) 2014-02-27 2015-09-04 안동과학대학교 산학협력단 Composition for having effect on suppression of bacterial soft rot disease and control method for the same
KR20160041084A (en) 2014-10-06 2016-04-18 재단법인 전남생물산업진흥원 Paenibacillus elgii 34-6 with antifungal activity to plant disease
US20160278388A1 (en) 2015-03-26 2016-09-29 Bayer Cropscience Lp Novel paenibacillus strain, antifungal compounds, and methods for their use
KR20170129921A (en) 2015-03-26 2017-11-27 바이엘 크롭사이언스 엘피 New Paranibacillus strains, antifungal compounds, and methods of use thereof

Non-Patent Citations (13)

* Cited by examiner, † Cited by third party
Title
Accession KCTC 13466BP (date of deposit: Jan. 23, 2018).
Chung et al., The Korean Journal of Pesticide Science, vol. 7, No. 1, pp. 32-37, 2003.
De Boer et al., "Influence of oxygen concentration end storage factors on susceptibility of potato tubers to bacterial soft rot (Erwinia carotovora)", Potato Res., 21, 1978, pp. 65-80.
GenBank Accession No. 113885, Nov. 10, 2011 (last modified Feb. 3, 2015).
International Search Report dated Feb. 8, 2019 in International Application No. PCT/KR/2018/001253, in 2 pages.
Kuo et al., "Associations between heat tolerance, water consumption, and morphological characters in Chinese cabbage", Euphytica, 39, pp. 85-73, 1988.
Kwon et al., "Isolation and Characterization of Plant Pathogen that Cause Soft Rot Disease in Napa Cabbage", Journal of Life Science, vol. 19, No. 8, pp. 1177-1182, 2809.
Lee, "Isolation and Cultural Condition of Paenibacillus elgii AM-67, and Characteristics of Antimicrobial Substance Against Chinese Cabbage (Brassica rapa subsp. pekinensis) Soft Rot", Thesis for the Degree of master of Science, Andong National University, Dec. 2016, in 78 pages.
Office Action dated Mar. 13, 2019 in Korean Application No. 10-2018-0010792, in 10 pages.
OPEÑA et al., "Breeding and Seed Production of Chinese cabbage in the Tropics and Subtropics", Technical Bulletin No. 17. Shanshua, Taiwan: Asian Vegetable Research and Development Center (AVRDC), May 1988, in 92 pages.
Shrestha et al., "Antagonistic Effect of Lactobacillus sp. Strain KLF01 Against Plant Pathogenic Bacteria Ralstonia solanacearum", The Korean Journal of Pesticide Science, vol. 13, No. 1, pp. 45-53, 2009.
Stommel et al., "Pepper (Capsicum annum) Soft Rot Caused by Erwinia carotovora subsp. atroseptica", Plant Disease, vol. 80, No. 10, Oct. 1996, pp. 1109-1112.
Wu et al., "Isolation and partial characterization of antibiotics produced by Paenibacillus elgii B69", FEMS Microbiol Lett, 310, pp. 32-38, 2019.

Also Published As

Publication number Publication date
KR20190090320A (en) 2019-08-01
KR102012583B1 (en) 2019-08-21
US20200187508A1 (en) 2020-06-18

Similar Documents

Publication Publication Date Title
Reva et al. Taxonomic characterization and plant colonizing abilities of some bacteria related to Bacillus amyloliquefaciens and Bacillus subtilis
US20030228679A1 (en) Compositions and methods for increasing plant growth by inoculation with bacillus strains
CN109714969B (en) Biological insecticide for potato late blight
Kantar et al. Chickpea: Rhizobium management and nitrogen fixation
Bathily et al. Bacillus pumilus, a new pathogen on potato tubers in storage in Mali
KR101845708B1 (en) New microorganism Bacillus velezensis YP2 or microbial agent comprising the same
KR20170127546A (en) Its use in the control of diseases caused by bacteria and fungi in strains and plants of Bacillus amyloliquefaciens.
Manici et al. The impact of legume and cereal cover crops on rhizosphere microbial communities of subsequent vegetable crops for contrasting crop decline
Vásconez et al. Evaluation of Bacillus megaterium strain AB4 as a potential biocontrol agent of Alternaria japonica, a mycopathogen of Brassica oleracea var. italica.
Lorite et al. Mesorhizobium olivaresii sp. nov. isolated from Lotus corniculatus nodules
US11800871B2 (en) Paenibacillus elgii AM-67 strain and biopesticide composition containing same
JP2009201459A (en) New microorganism, plant disease-control agent using the same and control method
KR20070010930A (en) Baciullus subtilis kmu-13 kccm 80020 having antifungal activity to plant disease-causing pathogens
Hidayah et al. Biological control potential of Trichoderma species and bacterial antagonists against Sclerotinia sclerotiorum on canola in Western Australia.
Amruta et al. Isolation and identification of rice blast disease-suppressing antagonistic bacterial strains from the rhizosphere of rice.
KR20180056474A (en) Novel compounds produced by Bacillus oryzicola YC7011 with activities of induced resistance against plant pathogens and insect and plant growth promotion
Munene et al. Management of bacterial wilt caused by Curtobacterium flaccumfaciens pv. flaccumfaciens in common bean (Phaseolus vulgaris) using rhizobacterial biocontrol agents
KR20110120748A (en) Bacillus amyloliquefaciens cp1 and contro method of strawberry anthracnose using the same
WO2019142963A1 (en) Novel paenibacillus elgii am-67 strain and environmentally friendly biopesticide composition containing same for controlling chinese cabbage soft rot disease, erwinia.sp.
Frémont et al. A comparison of Bradyrhizobium strains using molecular, cultural and field studies
JP2007153873A (en) Soilborne disease controlling agent
KR101211681B1 (en) Biological agent for plant diseases using streptomyces nigrogriseolus cmc0647
Akintokun et al. Potentials of indigenous Bacillus thuringiensis isolates from the soil in controlling Fusarium wilt of cucumber cause by Fusarium oxysporum f. sp cucumerinum
EP3855914A1 (en) Methods and compositions for bioprotection of potatoes from streptomyces scabies
WO2007055064A1 (en) Agent for controlling soil disease

Legal Events

Date Code Title Description
FEPP Fee payment procedure

Free format text: ENTITY STATUS SET TO UNDISCOUNTED (ORIGINAL EVENT CODE: BIG.); ENTITY STATUS OF PATENT OWNER: SMALL ENTITY

FEPP Fee payment procedure

Free format text: ENTITY STATUS SET TO SMALL (ORIGINAL EVENT CODE: SMAL); ENTITY STATUS OF PATENT OWNER: SMALL ENTITY

STPP Information on status: patent application and granting procedure in general

Free format text: APPLICATION DISPATCHED FROM PREEXAM, NOT YET DOCKETED

AS Assignment

Owner name: ANDONG NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, KOREA, REPUBLIC OF

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:KWON, GI SEOK;LEE, JUNG BOK;LEE, DAN BI;REEL/FRAME:052600/0546

Effective date: 20200427

STPP Information on status: patent application and granting procedure in general

Free format text: DOCKETED NEW CASE - READY FOR EXAMINATION

STPP Information on status: patent application and granting procedure in general

Free format text: NON FINAL ACTION MAILED

STPP Information on status: patent application and granting procedure in general

Free format text: RESPONSE TO NON-FINAL OFFICE ACTION ENTERED AND FORWARDED TO EXAMINER

STPP Information on status: patent application and granting procedure in general

Free format text: FINAL REJECTION MAILED

STPP Information on status: patent application and granting procedure in general

Free format text: NOTICE OF ALLOWANCE MAILED -- APPLICATION RECEIVED IN OFFICE OF PUBLICATIONS

STPP Information on status: patent application and granting procedure in general

Free format text: DOCKETED NEW CASE - READY FOR EXAMINATION

STPP Information on status: patent application and granting procedure in general

Free format text: NOTICE OF ALLOWANCE MAILED -- APPLICATION RECEIVED IN OFFICE OF PUBLICATIONS

STPP Information on status: patent application and granting procedure in general

Free format text: PUBLICATIONS -- ISSUE FEE PAYMENT VERIFIED

STCF Information on status: patent grant

Free format text: PATENTED CASE

CC Certificate of correction