US1015641A - Compounds of blood or its constituents and process of making same. - Google Patents

Description

Z5. c solved 1n blood are not, of course, capable of top amen pr :1

To all whom. it mm codwern:

Be itf ltnoiin 'that I, Earn) 'Scnnrrms, a citii'e'ii of 'tli eSiviss Confederation, and residifigi l it Basel, Switzerland, have invented a celtiii' ncw and useful Improved Gom- Constituents and of which the fol- Process of Making Same, lowiiigfis specification.

Flt silbject-matter of'my invention is a rot-ass ss: manufacturing compounds of loe d iji constituents thereof and aromatic stilfoliic' acids." Y

I have foundthat by allowing aromatic sulftiiiic ti'eids .e. g? guaiacol sulfonic acid, to act on blood or constituents thereof, I can obtairr/jflpublep compounds insoluble. in waterfroln the constituents of blood and the sulfonic-acidin question. I may add guaiacol sulfonic-acid as sucli, or I may liberate it from a mixture of blood or constituents thereof with sulfoguaiacate salts by means of stronger acids, when it usequently acts in stain nascemli.

I may employ blood of various origin and various nature. The inorganic salts distaking part in this reaction. My process is not lnmted to guanwol-sulfonic acid but, so

.far as has been observed hitherto, may be carried into practice with the aid' of any aromatic sulfonic-acid.

The new double-compounds are obtained by mixing blood or constituents thereof until a distinct acid reaction is obtained withthe sulfonic-acid at an ordinary temperature, and thereupon separating the precipitate obtained from the mother-lye,

New and therapeutically. valuable products are obtained according to my invention. They differ particularly sharply from all combinationsv of albumin with therapeutically operative substances heretofore employed.

My process fundamentally differs from those described in'the German Patents No. 84,551, Class 12 and No. 112,933, Class 30, because in these known processes it is a. matter, on the one hand, of a coagulation process in the presence of citric acid, on the. other hand, of the production of products -'split from blood by the action of powerful mineral acids. In contradistinction to these known processes, according to my invention 1111 the valuable constituents of blood with the cxception of the inorganic salts are obtained without a coagulation process and Application filed May 10, 1911. Serial No. 626,236.

STATES PATENT OFFICE.

.Enrrr.-.s'c1iEIrLIN, or BASEL, SWITZERLAND;

gpmgglgns; zroopfloal:rrs QONSTI'IUENTS AND PROCESS or MAKING SAME.

Patented Jan. 23, 1912.

without destruction in their genuine form as stable double compounds; It may be supposed that the aroinatic sulfonic-ac'ids anchor the Illn-lich'sside chains of the albumin molecules and preserve these from the changes to which they are always subject in the known process (by a. coagulation process or the action of acids). Further, it is generally well-known to bind therapeutically operative bodies to albumins, c. ovalbumin, blood albumin, in order to convert these i into a form insoluble'in gastric juice, so that the therapeutic action becomes operative only in the alkaline intcstinal juice; compare, for example, the German patent specifications 100,707, 88,029, Class 30 and. 102,050, Class 12", llmrmazc'ut. v(Dznfrallzall? 4-7 (1906), p. 354; under Torosan 'an'd Hagers Ilamlimclz. (701' Phat-1n. lraris, supplementary volume (1908), .p'ages 592 and 593 under Pilula Ilaemoglobini cum Guajacolo. In contradistinetiofl to these known processes, according to my invention Isolve the problem of obtaining the constitutents of the blood with the peculiarities peculiar to them by anchoring the albumin-like molecules in the blood with aromatic sulfonicacidswhich, as such, are therapeutically indifferent, whereas in the above-mentioned known processes the albumin molecule solely serves as a protective envelop for the therapeutically operative substances. Also, a -solution of sulfoguaiacate of potassium in blood-serum is known and therapeutically employed. See Hagers I1 mulbuch (Zer Iharm. I Ware-is, supplementary volume @908, page 12 under Tlhiocolscrum. This preparation isa simple mixture and no anchor? ing of the constituents of serum with guaiacol sulfonic acid takes place even under theaction of the hydrochloric acid of the gastric imperfectly indilute alkalis; they are insoluble in organic solvents and give no characteristic reactions with albumin reagents,

.chlorid of iron, permanganate of potassium and silver nitrate solution.

- The property of being insoluble in acids I but soluble in alkalis, however, leads to the.

conclusion that the bodies pass the stomach und nnposed in order to be-assimilated in the alkaline intestinal juice. Thebodies areunlimitedly durable, can be readily apportioned into doses andenable blood orconstitucnts thereof which have been made specilicall y operative by inoculation by the production or enricl'unent oi immune substances or anti-bodies to be administeredthrough the mouth, whereas the known tuberculin preparations can only be therapeutically cmploycd in the form of injections. Thus, for example, blood of bovine-vaccinated animals, anchored by aron'iatic sultonic-acids, retains its antituberculin properties unchanged for a long time.

.lfixzuuple: An aqueous solution of guaiacolsullonie-zuzid is allowed to flow into a portion of fresh animal blood, defibrinated or not, at an ordinary ten'i 'ierature while agi-. tating the same until the acid reaction to congo no longer disappears. The precipitated PlOtlllCl. is separated from the motherlyc, washed with water and dried at a temperature below 100" C.

I claim:--

l. The hereimlescribed process of making compounds of blood and its'constituents with aromatic sulfonic-acids, which comprises reacting on blood eonstituents'with an aromatic sulfouicacid.

2. The hereindescribed process of making compounds ofblood and its constituents with aromatic sulfonie-acids, which comprises reacting on blood constituents with an aqueous solution of guaiacol sulfonic-acid.

3. The herein described double compounds of a blood constituent and an aromatic sulionic-acid, which are stable, insoluble in water, dilute acids and dissolve slowly in dilute alkalis, and give no characteristic reaction with albumin reagents.

4:. The hereindescribed process, which comprises reacting on blood constituents of an inoculated animal with an aromatic sulfonicacid, separating the precipitate and drying the same below the destructive temperature for-the antitoxin. 1

5. The hereindescl'ibed process, which comprises reacting on blood constituents of an inoculated animal with a gnaiacol sulfonicacid, separating the/precipitate and drying the same below the-destructive temperature for the antitoxin.

In testimony whereof, I aflix my signature in'the presence of two witnesses.

I EMIL SCHEITLIN.

Witnesses: I

' SAMUEL I-IoLLINonR,

ARNOLD Zmsnn.