UA94373C2 - Method for chromatographic determination of total fat-acid composition of biological liquids - Google Patents
Method for chromatographic determination of total fat-acid composition of biological liquidsInfo
- Publication number
- UA94373C2 UA94373C2 UAA201009934A UAA201009934A UA94373C2 UA 94373 C2 UA94373 C2 UA 94373C2 UA A201009934 A UAA201009934 A UA A201009934A UA A201009934 A UAA201009934 A UA A201009934A UA 94373 C2 UA94373 C2 UA 94373C2
- Authority
- UA
- Ukraine
- Prior art keywords
- sample
- biological material
- biological
- acid composition
- solution
- Prior art date
Links
- 239000002253 acid Substances 0.000 title abstract 6
- 239000007788 liquid Substances 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 4
- 239000000203 mixture Substances 0.000 title abstract 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 abstract 6
- 239000012620 biological material Substances 0.000 abstract 5
- 239000000523 sample Substances 0.000 abstract 5
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 abstract 4
- WQDUMFSSJAZKTM-UHFFFAOYSA-N Sodium methoxide Chemical compound [Na+].[O-]C WQDUMFSSJAZKTM-UHFFFAOYSA-N 0.000 abstract 4
- 150000007513 acids Chemical class 0.000 abstract 3
- 150000002632 lipids Chemical group 0.000 abstract 3
- 239000012071 phase Substances 0.000 abstract 3
- 239000012472 biological sample Substances 0.000 abstract 2
- 210000004369 blood Anatomy 0.000 abstract 2
- 239000008280 blood Substances 0.000 abstract 2
- 238000004364 calculation method Methods 0.000 abstract 2
- 150000005217 methyl ethers Chemical class 0.000 abstract 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 abstract 2
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 1
- 229910052796 boron Inorganic materials 0.000 abstract 1
- 238000003965 capillary gas chromatography Methods 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 230000003111 delayed effect Effects 0.000 abstract 1
- 238000009795 derivation Methods 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 150000002170 ethers Chemical class 0.000 abstract 1
- 239000000284 extract Substances 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- JHHZQADGLDKIPM-UHFFFAOYSA-N hept-3-en-2-one Chemical compound CCCC=CC(C)=O JHHZQADGLDKIPM-UHFFFAOYSA-N 0.000 abstract 1
- 238000011835 investigation Methods 0.000 abstract 1
- 239000007791 liquid phase Substances 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 150000004702 methyl esters Chemical class 0.000 abstract 1
- 230000011987 methylation Effects 0.000 abstract 1
- 238000007069 methylation reaction Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 238000013517 stratification Methods 0.000 abstract 1
- 238000000859 sublimation Methods 0.000 abstract 1
- 230000008022 sublimation Effects 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to field of medicine, in particular to clinical-laboratory studies and can be used for determination of fat acid composition of lipids in biological samples of different origin. Method for chromatographic determination of total fat acid composition of biological liquids prescribes separation of lipids from matrix material, at that liquid sample of biological material chosen from group: blood-serum, suspension of cells homogenizate of tissue is dried to obtain sample of de-watered biological material in apparatus of sublimation drying or in rotary vacuum concentrator of type “Savant SpeedVac”, at that aliquote of sample of liquid biological material under investigation in amount of 20-500 ml is de-watered directly in test-tube for derivation, and lipids of dried sample of biological material in that very test-tube are transformed to respective methyl esters with heating with solution of 0.5 N of sodium methylate in methanol in calculation of 150 mkl of solution of sodium methylate oer 100 mkl of blood or 15 % of homogenizate, and free fat acids present are heated with 10 % methanol solution of tri-fluoric boron in calculation of 0.8-1.0 ml per 100 ml of blood or 15 % homogenizate, at that after the end of methylation alcohol solution of sample of biological material is dissolved with distillated water in two times, after that one extracts methyl ethers of fat acids obtained to heptane non-polar phase, at that somewhat delayed stratification of water-alcohol and non-polar heptane phase at stage of extraction of ethers from reaction nix is accelerated by adding drop of propyl alcohol or by shirt-term (1-2 minutes) centrifugation in low-speed centrifuge, after that extracted to heptane phase methyl ethers of fat acids are determined quantitatively by method of capillary gas chromatography in column with polar immobilized liquid phase. Main advantage of the method proposed by us is in principal simplification and speed-up of preparation of biological samples for chromatographic analysis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAA201009934A UA94373C2 (en) | 2010-08-10 | 2010-08-10 | Method for chromatographic determination of total fat-acid composition of biological liquids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAA201009934A UA94373C2 (en) | 2010-08-10 | 2010-08-10 | Method for chromatographic determination of total fat-acid composition of biological liquids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA94373C2 true UA94373C2 (en) | 2011-04-26 |
Family
ID=50832475
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UAA201009934A UA94373C2 (en) | 2010-08-10 | 2010-08-10 | Method for chromatographic determination of total fat-acid composition of biological liquids |
Country Status (1)
| Country | Link |
|---|---|
| UA (1) | UA94373C2 (en) |
-
2010
- 2010-08-10 UA UAA201009934A patent/UA94373C2/en unknown
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