UA90935U - Process for production of thrombin - Google Patents
Process for production of thrombinInfo
- Publication number
- UA90935U UA90935U UAU201401076U UAU201401076U UA90935U UA 90935 U UA90935 U UA 90935U UA U201401076 U UAU201401076 U UA U201401076U UA U201401076 U UAU201401076 U UA U201401076U UA 90935 U UA90935 U UA 90935U
- Authority
- UA
- Ukraine
- Prior art keywords
- proserosyme
- sepharose
- vitamin
- buffer solution
- blood plasma
- Prior art date
Links
- 108090000190 Thrombin Proteins 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 229960004072 thrombin Drugs 0.000 title abstract 3
- 230000001419 dependent effect Effects 0.000 abstract 4
- 108010017384 Blood Proteins Proteins 0.000 abstract 3
- 102000004506 Blood Proteins Human genes 0.000 abstract 3
- 229930003448 Vitamin K Natural products 0.000 abstract 3
- 239000007853 buffer solution Substances 0.000 abstract 3
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 abstract 3
- 210000002381 plasma Anatomy 0.000 abstract 3
- 235000019168 vitamin K Nutrition 0.000 abstract 3
- 239000011712 vitamin K Substances 0.000 abstract 3
- 150000003721 vitamin K derivatives Chemical class 0.000 abstract 3
- 229940046010 vitamin k Drugs 0.000 abstract 3
- 229920002684 Sepharose Polymers 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- 230000002745 absorbent Effects 0.000 abstract 2
- 239000002250 absorbent Substances 0.000 abstract 2
- TZCXTZWJZNENPQ-UHFFFAOYSA-L barium sulfate Chemical compound [Ba+2].[O-]S([O-])(=O)=O TZCXTZWJZNENPQ-UHFFFAOYSA-L 0.000 abstract 2
- 239000012141 concentrate Substances 0.000 abstract 2
- 238000010828 elution Methods 0.000 abstract 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 abstract 1
- 238000007445 Chromatographic isolation Methods 0.000 abstract 1
- 108060005987 Kallikrein Proteins 0.000 abstract 1
- 102000001399 Kallikrein Human genes 0.000 abstract 1
- 239000012614 Q-Sepharose Substances 0.000 abstract 1
- 230000004913 activation Effects 0.000 abstract 1
- 239000003593 chromogenic compound Substances 0.000 abstract 1
- 108010077727 ecamulin Proteins 0.000 abstract 1
- 238000005194 fractionation Methods 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
Landscapes
- Peptides Or Proteins (AREA)
Abstract
The process for production of thrombin comprises production of fraction of vitamin K-dependent blood plasma proteins with subsequent chromatographic isolation of proserosyme from this fraction, and activation of proserosyme into thrombin. At first concentrate of vitamin K-dependent blood plasma proteins is produced by modified method by sorption thereof on BaSO4 with subsequent elution of K-dependent proteins with 0.05 M tris-HCl buffer solution, pH 7.4. Proserosyme is isolated from the concentrate of vitamin K-dependent blood plasma proteins by separation on Q-Sepharose ("Sigma"), which is balanced with buffer solution and subsequent elution of proserosyme with this buffer solution with addition of 0.4 M NaCl. Immobilization of ecamulin on absorbent BrCN-Sepharose ("SIGMA-AlDRICH") is carried out, the produced absorbent ecamulin-Sepharose is standardized according to chromogenic substrate of kallikrein S2302.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAU201401076U UA90935U (en) | 2014-02-05 | 2014-02-05 | Process for production of thrombin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAU201401076U UA90935U (en) | 2014-02-05 | 2014-02-05 | Process for production of thrombin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA90935U true UA90935U (en) | 2014-06-10 |
Family
ID=89901579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UAU201401076U UA90935U (en) | 2014-02-05 | 2014-02-05 | Process for production of thrombin |
Country Status (1)
| Country | Link |
|---|---|
| UA (1) | UA90935U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021194461A1 (en) * | 2020-06-22 | 2021-09-30 | Palladin Instytute Of Biochemistry Of The National Academy Of Sciences Of Ukraine | The method of production of a haemostatic agent and a haemostatic agent for major bleeding control |
-
2014
- 2014-02-05 UA UAU201401076U patent/UA90935U/en unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021194461A1 (en) * | 2020-06-22 | 2021-09-30 | Palladin Instytute Of Biochemistry Of The National Academy Of Sciences Of Ukraine | The method of production of a haemostatic agent and a haemostatic agent for major bleeding control |
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