UA87245C2 - Method for cultivating unicellular green algae haematococcus pluvialis for producing astaxanthin - Google Patents

Method for cultivating unicellular green algae haematococcus pluvialis for producing astaxanthin

Info

Publication number
UA87245C2
UA87245C2 UAA200806137A UAA200806137A UA87245C2 UA 87245 C2 UA87245 C2 UA 87245C2 UA A200806137 A UAA200806137 A UA A200806137A UA A200806137 A UAA200806137 A UA A200806137A UA 87245 C2 UA87245 C2 UA 87245C2
Authority
UA
Ukraine
Prior art keywords
monm
astaxanthine
green algae
haematococcus pluvialis
unicellular green
Prior art date
Application number
UAA200806137A
Other languages
Russian (ru)
Ukrainian (uk)
Inventor
Галина Семеновна Минюк
Наталья Викторовна Терентьева
Ирина Викторовна Дробецкая
Ирина Николаевна Чубчикова
Original Assignee
Институт Биологии Южных Морей Им. А.О. Ковалевского Нан Украины
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Институт Биологии Южных Морей Им. А.О. Ковалевского Нан Украины filed Critical Институт Биологии Южных Морей Им. А.О. Ковалевского Нан Украины
Priority to UAA200806137A priority Critical patent/UA87245C2/en
Publication of UA87245C2 publication Critical patent/UA87245C2/en

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Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management

Abstract

The invention relates to a method for growing the unicellular green algae Haematococcus pluvialis for obtaining astaxanthine which provides for inducing biosynthesis of astaxanthine in monade vegetative cells with the grown on the nutrient medium MONM-1, in the state of substrate saturation of cells according to biogenic elements (inoculate) introduced in an amount of 0.3-0.3510cellslinto the nutrient medium MONM-2 which differs from the medium MONM-1 by the content of nitrogen (0.2 mMl) and phosphorus (0.12 mMl) lowered by 30 times. Sodium acetate, 15 mM, is added singly and the further growing in the course of 20 days is carried out in a semi-flowing regime (0.1-0.3 day) maintaining in the medium MONM-2 the preset level of nitrogen and phosphorus at round-the-clock lighting by daylight luminescent lamps with an intensity of light flow of 120 μЕms, continuous air flushing (0.3 lmin) and temperature of 22-26 °С. An economical and effective method that can be laid into the base of industrial cultivation of the unicellular green algae Haematococcus pluvialis is developed as a raw material for obtaining a natural astaxanthine as a pure preparation and a biologically active bad with the increased bioavailability of a carotenoide. The method excludes transforming the vegetative cells into aplanospores, provides with an advantage in cultures of monade structure (>80%) in the course of not less than 20 days and the content of astaxanthine in the biomass is not less than 2% of dry matter.
UAA200806137A 2008-05-12 2008-05-12 Method for cultivating unicellular green algae haematococcus pluvialis for producing astaxanthin UA87245C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
UAA200806137A UA87245C2 (en) 2008-05-12 2008-05-12 Method for cultivating unicellular green algae haematococcus pluvialis for producing astaxanthin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
UAA200806137A UA87245C2 (en) 2008-05-12 2008-05-12 Method for cultivating unicellular green algae haematococcus pluvialis for producing astaxanthin

Publications (1)

Publication Number Publication Date
UA87245C2 true UA87245C2 (en) 2009-06-25

Family

ID=50632026

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA200806137A UA87245C2 (en) 2008-05-12 2008-05-12 Method for cultivating unicellular green algae haematococcus pluvialis for producing astaxanthin

Country Status (1)

Country Link
UA (1) UA87245C2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108753620A (en) * 2018-05-30 2018-11-06 昆明理工大学 A method of improving haematococcus pluvialis biomass and content astaxanthin
CN109679853A (en) * 2019-01-18 2019-04-26 昆明理工大学 The method for improving haematococcus pluvialis biomass and astaxanthin yield using fulvic acid

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108753620A (en) * 2018-05-30 2018-11-06 昆明理工大学 A method of improving haematococcus pluvialis biomass and content astaxanthin
CN109679853A (en) * 2019-01-18 2019-04-26 昆明理工大学 The method for improving haematococcus pluvialis biomass and astaxanthin yield using fulvic acid

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