UA84668C2 - Method for obtaining of oily body conjugated with recombinant multimeric protein complex - Google Patents

Method for obtaining of oily body conjugated with recombinant multimeric protein complex

Info

Publication number
UA84668C2
UA84668C2 UA2003076823A UA2003076823A UA84668C2 UA 84668 C2 UA84668 C2 UA 84668C2 UA 2003076823 A UA2003076823 A UA 2003076823A UA 2003076823 A UA2003076823 A UA 2003076823A UA 84668 C2 UA84668 C2 UA 84668C2
Authority
UA
Ukraine
Prior art keywords
recombinant polypeptide
obtaining
multimeric protein
protein complex
oil
Prior art date
Application number
UA2003076823A
Other languages
Russian (ru)
Ukrainian (uk)
Inventor
Роейен Гейс ван
Харм Декерз
Петер Бернард Хайфетц
Стивен П. Бриггз
Бипин Кумар Далмиа
Вал Грегг Дель
Стив Заплачински
Морис Молони
Original Assignee
Сембайосиз Джинетикс Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Сембайосиз Джинетикс Инк. filed Critical Сембайосиз Джинетикс Инк.
Publication of UA84668C2 publication Critical patent/UA84668C2/en

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Описані способи одержання олійних тілець, зв'язаних з рекомбінантними мультимерними білковими комплексами, такими як редокс-білки і імуноглобуліни. Редокс-білок є ферментативно активним при одержанні у пов'язаному з олійними тільцями вигляді. Спосіб включає одержання у клітині, що містить олійні тільця, першого рекомбінантного поліпептиду і другого рекомбінантного поліпептиду. Вказаний перший рекомбінантний поліпептид здатний зв'язуватись з вказаним другим рекомбінантним поліпептидом з утворенням мультимерного білкового комплексу. Далі спосіб включає зв’язування вказаного мультимерного білкового комплексу з олійним тільцем через націлюючий на олійні тільця білок, здатний зв'язуватись з вказаним олійним тільцем і вказаним першим рекомбінантним поліпептидом. Представлені також химерні послідовності нуклеїнових кислот, які кодують мультимерні злиті білки та білки, клітини, рослини і композиції, що мають до них відношення.Methods of obtaining oil bodies associated with recombinant multimeric protein complexes, such as redox proteins and immunoglobulins, are described. Redox protein is enzymatically active in the preparation in an associated with oil bodies. The method involves obtaining in a cell containing oil bodies, the first recombinant polypeptide and the second recombinant polypeptide. Said first recombinant polypeptide is capable of binding to said second recombinant polypeptide to form a multimeric protein complex. Further, the method involves binding said multimeric protein complex to an oil body through an oil-targeting protein capable of binding to said oil body and said first recombinant polypeptide. Chimeric nucleic acid sequences encoding multimeric fusion proteins and proteins, cells, plants, and related compositions are also provided.

UA2003076823A 2000-12-19 2001-12-19 Method for obtaining of oily body conjugated with recombinant multimeric protein complex UA84668C2 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US74290000A 2000-12-19 2000-12-19

Publications (1)

Publication Number Publication Date
UA84668C2 true UA84668C2 (en) 2008-11-25

Family

ID=34116883

Family Applications (1)

Application Number Title Priority Date Filing Date
UA2003076823A UA84668C2 (en) 2000-12-19 2001-12-19 Method for obtaining of oily body conjugated with recombinant multimeric protein complex

Country Status (2)

Country Link
UA (1) UA84668C2 (en)
ZA (1) ZA200305570B (en)

Also Published As

Publication number Publication date
ZA200305570B (en) 2004-09-03

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