UA77819C2 - Appliance for freezing and storing large volumes of biological materials - Google Patents

Abstract

The invention relates to appliances for quick freezing and keeping of biological materials (erythrocytes and sperm) in medium of refrigerantt. Appliance for freezing and keeping large volumes of biological materials includes tube-capillary made of polymer transparent non-toxic material filled with biological material diluted in medium including up to 10 % of cryo-protector, frozen in liquid nitrogen and placed to Dewar vessel. Filled tube-capillary is placed in slots of cross elements by spiral from the edge to the center of the stand. Tube-capillary has depressions in width about one centimeter to connection of opposite walls in each ten centimeters. The stand has long slots to place cross elements with slots for tube-capillary and ring for tightening. The invention provides quick freezing and keeping of biological agents in liquid nitrogen with use of 10 % of cryo-protector without loss of quality.

Description

Description of the invention

The invention relates to devices for rapid freezing and storage in a refrigerant environment of 2 biological materials (erythrocytes and sperm).

Known device (a.s. USSR Mo431805 MKV E 250 3/10 Chamber for freezing biological materials /

B.Y. Verkin, Yu.M. Rudko, V.Ya. Ostypov, V.A. Serebnytskyi, A.E. Kiselev. - Mo1773952/28-13; Application 04/17/72;

Publ. 15.03.76. - Bull. 10), which includes the introduction of large volumes of biological objects into the chamber, the inner wall of the body and the container of which are made cylindrical with a rounded bottom, and spiral guides are placed on the inner wall of the body 70.

The disadvantage of this device is that the large volume of the container does not provide an opportunity to get rid of significant temperature gradients in the biological material, which does not allow it to be quickly frozen and thawed and leads to significant losses in the quality of the object.

The invention aims to ensure rapid freezing and storage in liquid nitrogen, which is in a Dewar vessel, of large portions of biological materials diluted in a medium containing up to 1095 cryoprotectants without loss of quality.

The task is achieved by the fact that in the device for freezing and storing large volumes of biological materials, which includes a capillary tube made of polymer transparent non-toxic material filled with biological material diluted in a medium containing up to 1095 cryoprotectant, frozen in liquid nitrogen and placed in the Dewar vessel, according to the invention, a filled capillary tube is placed in the grooves of the crosses fixed with a ring on a rack with longitudinal mutually perpendicular grooves.

Figure 1 shows the general view of the proposed device, Figure 2 shows the device in axonometry.

The device for freezing and storing large volumes of biological materials has a rack 1 with longitudinal mutually perpendicular grooves 2, in which cross-pieces C are attached with grooves 4 for placing a capillary tube 5, rack 1 with several cross-pieces Z is fixed with a ring 6 for tightening on different height Gee)

Before freezing, the first lower cross is installed in the grooves of 2 racks 1 at the required height.

The capillary tube 5 is placed in the grooves 4 of the crosspiece 3, starting from the rack 1 in a spiral. After the last turn of the capillary tube 5 is completed, the next cross-piece C is installed and the capillary tube 5 is placed in its grooves 4 in a spiral from the edge to the center of the rack 1. In this way, the required number of cross-piece floors is collected until the entire capillary tube 5 is completely placed. To fasten the crossbars Z in the grooves 2 of the rack 1 on its end "- put on the ring b for tightening. Filling the capillary tube 5 with material for freezing can be carried out both before placement and after its placement in the grooves 4 of the crosspiece 3.

The entire device for freezing and storing biological objects is constructively assembled and fixed using frictional forces. The device is easily assembled and disassembled from the same type of crosspieces and rack. The volume of the freezing material is regulated by the number of crosses 3, which determines the distance between them on the rack 1 and the length of the capillary tube 5.

To take into account volume expansion during freezing of the liquid, the capillary tube 5 is deformed every ten centimeters by squeezing it to a width of about one centimeter to the connection of the opposite walls, which prevents the rupture of the capillary due to their straightening in the process of freezing the biological material. 70 The use of this device allows, thanks to the large area of heat transfer, to quickly freeze and unfreeze biological objects using 1095 cryoprotector. In the case of using other containers, the amount of cryoprotectant must be increased to 2095, since freezing and thawing takes place at a lower speed. Such increased concentrations of cryoprotectants are toxic and cannot be administered to humans and animals. -AND

Claims (1)

The formula of the invention is a device for freezing and storing large volumes of biological materials, which includes a 20 capillary tube made of polymer transparent non-toxic material, filled with biological material diluted in a medium containing up to 1095 cryoprotectant, frozen in liquid nitrogen and placed in the middle of the Dewar vessel, which differs in that a filled capillary tube, the length of which is determined by the volume of freezing of biological materials, is placed in the grooves of the cross-pieces in a spiral from the edge to the center of the rack, the capillary tube has a depression about one centimeter wide to the connection of opposite walls every 2o ten centimeters, the rack has longitudinal grooves for placing crosspieces and a ring for tightening, and in Ф! Grooves for a capillary tube are made of crosses. name) 60 b5