UA71282A - An apparatus for growing cells - Google Patents

Abstract

An apparatus for growing cells contains cylindrical tight capacity with process branch pipes and mechanism for mixing medium disposed in the middle of capacity, and comprising vertical rod with drive installed on axis with an opportunity of reciprocal movement in the vertical plane and a set of mixing elements. Mixing elements represent thin elastic membranes disposed on radial supports .

Description

Description of the invention

The invention relates to biotechnology, pharmacy and industrial microbiology and can be used in 2 continuous or periodic processes of obtaining biomass and biologically active substances synthesized by cells of animal origin.

There are well-known designs of devices with a vibrating mixing system, the feature of which is the presence of a movable rod with a package of contact elements. The rod performs reciprocating movement in the vertical plane (Authority of the USSR Mo755835, class C1281/10, publ. 15.08.80. Byul. Mo30; authort. USSR Mo759586, class C12S1/10, publ. 70 30.08. 80. Bul. Mo32).

The disadvantages of the listed devices are: the limited possibility of their use for the cultivation of cells of animal origin, due to the high saturation of the nutrient medium with the gas phase, which adversely affects the vital activity of animal cells. In the devices, the issue of guaranteed sealing has not been resolved, which is unacceptable for biosynthesis in aseptic conditions. Most of the bioreactors with vibration 12 mixing are oriented to large concentrations of microorganism cells and, as a result, to a high level of mass exchange processes, which is achieved by the introduction of significant energy for mixing and aeration. At a high intensity of mixing, significant shear stresses occur in the area of movement of the contact elements, which have a negative effect on the cells, damaging their shell.

According to the design, the prototype is an apparatus for growing microorganisms and cells (Authority of the USSR Mo1773936 kl, С12М1/04, publ. 07.11.92. Bull. Mo41). The device is made in the form of a vertical container in which a rod moves with flexible plates of uniform or variable cross-section fixed on it, which have slots that segment them into separate elements. The movement of the rod is caused by an external source of oscillations. During the reciprocating movement of the rod with a package of plates, the latter bend due to the resistance of the liquid. Slots and a variable cross-section reduce the stiffness of the plates and create additional turbulence zones.

The disadvantage of this device is the relatively high speed of movement of the interacting phases, which does not allow to create soft "mixing conditions under which the cells are washed with the nutrient medium, necessary for the cultivation of animal cells.

The invention is based on the task of creating an apparatus for growing cells, which makes it possible to increase the yield of target products and biomass due to more complete and careful mixing. high school

The task is achieved by the fact that the proposed device for growing cells contains a cylindrical hermetic container with technological nozzles and a device for mixing the medium located in the middle of the container, which includes a vertical rod with a drive installed on the axis of the container with the possibility of reciprocating movement in the vertical planes and a set of stirring elements, according to the invention, which are elastic thin membranes located on radial supports.

The causal relationship between the proposed features and the expected technical result is shown below.

To achieve the required effect of soft mixing, membranes made of elastic, inert and heat-resistant material are used. The choice of material is determined by the possibility of using it as a carrier for "immobilization of inducers of the synthesis of biologically active substances." When the rod with the package of membranes moves down, the latter rise up. When the rod with the package of membranes moves up, the latter, resting on the radial supports, create rarefaction in the lower part of the apparatus, due to which the cells are transferred to

From" weighted state. The combination of the elasticity of the membranes and the necessary intensity of oscillations makes it possible to exclude the inhibitory effect of the resulting stresses on the cell.

Figure 1 schematically shows the proposed device.

The apparatus consists of a hermetic container 1 and is equipped with a fitting 2 and a filter C for supplying and sterilizing air, a fitting 4 and a filter 5 for removing and cleaning the used air, a fitting b - for loading the nutrient medium and seed material, a device 7 for temperature stabilization, a sampler 8, fitting 9 for supplying cooling water, fitting 10 for discharging the contents of the device. Inside the device there is a vertical rod 11 with a package of elastic membranes 12, located on 20 radial supports 13. Cooling water is discharged through a fitting 14. Aeration and foam suppression is carried out by a rigid perforated membrane 15, which has conical holes. The hermetic connection of the rod 11 with the container 1 is ensured by the bellows 16.

The device works as follows.

Sterile nutrient medium and seed material are supplied to the device after thermal sterilization. Sterile air for gas exchange is supplied to the upper part of the apparatus through the 29 fitting 2 and filter C. Worked in air exits the device through connector 4. The rod 11 is connected to the external oscillator and makes a reciprocating movement, along with it the elastic membranes located on the radial supports move.

Elastic membranes on radial supports are located evenly along the height of the device. During vibration 60 mixing, the lower elastic membrane, located on the radial supports, is located near the bottom of the apparatus and serves to lift the settling cells. Each of the elastic membranes, located on the radial supports, performs the role of a transport element, directing the cells to the aeration zone.

An increase in the output of the target product is achieved as a result of the formation of a hydrodynamic regime, which provides more perfect mixing and elimination of stagnant zones in the apparatus.

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Fig 1

Claims (1)

Formulation of the invention Apparatus for growing cells, containing a cylindrical hermetic container with technological nozzles and a device for mixing the medium located inside the container, which includes a vertical rod with a drive installed on the axis of the container with the possibility of reciprocating movement in the vertical plane and "a set of mixing elements, which differs in that the mixing elements are elastic thin membranes located on radial supports. Official bulletin "Industrial Property". Book 1 "Inventions, useful models, topographies of integrated EM microcircuits", 2004, M 11, 11/15/2004. State Department of Intellectual Property of the Ministry of Education and Social Science of Ukraine. with - and - - and? -i - name) (ee) Ko) 60 b5