UA4222U - Method for preparation of purified avian tuberculin (ppd) - Google Patents

Method for preparation of purified avian tuberculin (ppd)

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Publication number
UA4222U
UA4222U UA2004031938U UA2004031938U UA4222U UA 4222 U UA4222 U UA 4222U UA 2004031938 U UA2004031938 U UA 2004031938U UA 2004031938 U UA2004031938 U UA 2004031938U UA 4222 U UA4222 U UA 4222U
Authority
UA
Ukraine
Prior art keywords
ppd
preparation
culture
purified
tuberculin
Prior art date
Application number
UA2004031938U
Other languages
Russian (ru)
Ukrainian (uk)
Inventor
Андрій Іванович Завгородній
Андрей Иванович Завгородний
Юрій Якович Кассіч
Юрий Яковлевич Кассич
Наталія Василівна Калашник
Наталия Васильевна Калашник
Світлана Аркадіївна Позмогова
Светлана Аркадьевна Позмогова
Борис Тимофійович Стегній
Борис Тимофеевич Стегний
Original Assignee
Інститут Експериментальної І Клінічної Ветеринарної Медицини
Институт Экспериментальной И Клинической Ветеринарной Медицины
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Інститут Експериментальної І Клінічної Ветеринарної Медицини, Институт Экспериментальной И Клинической Ветеринарной Медицины filed Critical Інститут Експериментальної І Клінічної Ветеринарної Медицини
Priority to UA2004031938U priority Critical patent/UA4222U/en
Publication of UA4222U publication Critical patent/UA4222U/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The method for the preparation of the purified avian tuberculin (PPD) comprises the culture of the proteinogenous strain of M. avium in the synthetic culture medium, the inactivation of the bacterial mass, the filtration, the separation of the culture filtrate, the precipitation of the protein by trichloroacetic acid (final concentration of 6%), and the isolation of the final product. The culture filtrate is concentrated on the membrane retaining the proteins of 15 kDa. The product is additionally purified by ammonium sulfate pH 7.0-7.2 and sterilized in Sartorius apparatus.
UA2004031938U 2004-03-16 2004-03-16 Method for preparation of purified avian tuberculin (ppd) UA4222U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
UA2004031938U UA4222U (en) 2004-03-16 2004-03-16 Method for preparation of purified avian tuberculin (ppd)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
UA2004031938U UA4222U (en) 2004-03-16 2004-03-16 Method for preparation of purified avian tuberculin (ppd)

Publications (1)

Publication Number Publication Date
UA4222U true UA4222U (en) 2005-01-17

Family

ID=74347882

Family Applications (1)

Application Number Title Priority Date Filing Date
UA2004031938U UA4222U (en) 2004-03-16 2004-03-16 Method for preparation of purified avian tuberculin (ppd)

Country Status (1)

Country Link
UA (1) UA4222U (en)

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