Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Preparation Of Compounds By Using Micro-Organisms
(AREA)
Enzymes And Modification Thereof
(AREA)
Abstract
A method for purification of cytochrome R450 aromatase provides for addition of equal volume of agarose suspension into extract before elution, to which joined is covalently 17-methytestosteron through spacer –siccine acid in tris-HCl buffer at pH of 7.3 – 7.5, ageing for 3 -7 minutes at the temperature of 35 – 40 °C with subsequent centrifugation for 5 minutes at 10000 g. The invention relates to the biochemistry and may be used in enzyme technology, biochemical and obstetric-gynecologic researches.
UA2000106091A2000-10-302000-10-30method for purification of cytochrome R450 aromatase
UA42198A
(en)