UA35599C2 - Method for purifying hemoglobin from raw solution - Google Patents
Method for purifying hemoglobin from raw solutionInfo
- Publication number
- UA35599C2 UA35599C2 UA95058439A UA95058439A UA35599C2 UA 35599 C2 UA35599 C2 UA 35599C2 UA 95058439 A UA95058439 A UA 95058439A UA 95058439 A UA95058439 A UA 95058439A UA 35599 C2 UA35599 C2 UA 35599C2
- Authority
- UA
- Ukraine
- Prior art keywords
- hemoglobin
- column
- admixtures
- raw solution
- stage
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/422—Displacement mode
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to the chromatographic purification of hemoglobin. The method for purifying hemoglobin from the raw solution containing the admixture of proteinaceous origin provides for the use of ion-exchange chromatography. The raw solution containing 0.1-20% hemoglobin. At the first stage, anion-exchanging column is used when the admixtures are more acidic than hemoglobin, or cation-exchanging column is used when the admixtures are more alkaline than hemoglobin. The continuous flow of the solution results first in the complete saturation of the column with hemoglobin and the admixtures with high affinity to the column, then the column is overloaded following the substitution of hemoglobin. At the second stage, the ion-exchanger alternative to that used at the first stage is employed. Again, upon the complete saturation and overloading of the column, hemoglobin is substituted.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002106612A CA2106612C (en) | 1993-09-21 | 1993-09-21 | Displacement chromatography process |
PCT/CA1994/000514 WO1995008574A1 (en) | 1993-09-21 | 1994-09-20 | Displacement chromatography process and purified hemoglobin product |
Publications (1)
Publication Number | Publication Date |
---|---|
UA35599C2 true UA35599C2 (en) | 2001-04-16 |
Family
ID=4152343
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
UA95058439A UA35599C2 (en) | 1993-09-21 | 1994-09-20 | Method for purifying hemoglobin from raw solution |
Country Status (9)
Country | Link |
---|---|
JP (1) | JP4057049B2 (en) |
KR (1) | KR100361894B1 (en) |
AT (1) | ATE175975T1 (en) |
DE (1) | DE69416103T2 (en) |
DK (1) | DK0668877T3 (en) |
ES (1) | ES2126775T3 (en) |
HU (1) | HU218159B (en) |
RU (1) | RU2145873C1 (en) |
UA (1) | UA35599C2 (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8153009B2 (en) * | 2005-04-22 | 2012-04-10 | Waters Technologies Corporation | Apparatus and methods for mass-spectrometric directed purification of biopolymers |
SI2462158T1 (en) | 2009-08-06 | 2018-04-30 | F. Hoffmann-La Roche Ag | Method to improve virus removal in protein purification |
US20130331554A1 (en) * | 2010-11-15 | 2013-12-12 | Biogen Idec Inc. | Enrichment and concentration of select product isoforms by overloaded bind and elute chromatography |
ES2697676T3 (en) * | 2011-12-22 | 2019-01-25 | Hoffmann La Roche | Ion exchange membrane chromatography |
EP3301115B1 (en) * | 2013-05-06 | 2021-11-10 | Sanofi | Continuous multistep process for purifying antibodies |
TW202128264A (en) * | 2019-09-25 | 2021-08-01 | 瑞士商赫孚孟拉羅股份公司 | Cation chromatography using predicted elution buffer salt concentration |
CN114712894B (en) * | 2022-04-08 | 2024-04-30 | 无锡博慧斯生物医药科技有限公司 | Activating solution, activating method and application of glycosylated hemoglobin chromatographic column |
-
1994
- 1994-09-20 DK DK94926756T patent/DK0668877T3/en active
- 1994-09-20 ES ES94926756T patent/ES2126775T3/en not_active Expired - Lifetime
- 1994-09-20 HU HU9501479A patent/HU218159B/en not_active IP Right Cessation
- 1994-09-20 DE DE69416103T patent/DE69416103T2/en not_active Expired - Fee Related
- 1994-09-20 JP JP50945895A patent/JP4057049B2/en not_active Expired - Fee Related
- 1994-09-20 UA UA95058439A patent/UA35599C2/en unknown
- 1994-09-20 AT AT94926756T patent/ATE175975T1/en not_active IP Right Cessation
- 1994-09-20 RU RU95112503A patent/RU2145873C1/en not_active IP Right Cessation
- 1994-09-21 KR KR1019950701487A patent/KR100361894B1/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
RU2145873C1 (en) | 2000-02-27 |
DE69416103T2 (en) | 1999-08-05 |
JPH08503714A (en) | 1996-04-23 |
HUT72924A (en) | 1996-06-28 |
JP4057049B2 (en) | 2008-03-05 |
DK0668877T3 (en) | 1999-09-13 |
HU9501479D0 (en) | 1995-07-28 |
KR100361894B1 (en) | 2003-05-12 |
RU95112503A (en) | 1996-12-20 |
ES2126775T3 (en) | 1999-04-01 |
HU218159B (en) | 2000-06-28 |
ATE175975T1 (en) | 1999-02-15 |
DE69416103D1 (en) | 1999-03-04 |
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