UA35599C2 - Method for purifying hemoglobin from raw solution - Google Patents

Method for purifying hemoglobin from raw solution

Info

Publication number
UA35599C2
UA35599C2 UA95058439A UA95058439A UA35599C2 UA 35599 C2 UA35599 C2 UA 35599C2 UA 95058439 A UA95058439 A UA 95058439A UA 95058439 A UA95058439 A UA 95058439A UA 35599 C2 UA35599 C2 UA 35599C2
Authority
UA
Ukraine
Prior art keywords
hemoglobin
column
admixtures
raw solution
stage
Prior art date
Application number
UA95058439A
Other languages
Russian (ru)
Ukrainian (uk)
Inventor
Дайана Х. Плайура
Дайен Е. Уіффен
Дайен Е. Уиффен
Селмен Ешраф
Селмен Эшраф
Ентоні А. Мегнін
Энтони А. Мегнин
Original Assignee
Хемосол Інк
Хемосол Инк
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA002106612A external-priority patent/CA2106612C/en
Application filed by Хемосол Інк, Хемосол Инк filed Critical Хемосол Інк
Publication of UA35599C2 publication Critical patent/UA35599C2/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/422—Displacement mode
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to the chromatographic purification of hemoglobin. The method for purifying hemoglobin from the raw solution containing the admixture of proteinaceous origin provides for the use of ion-exchange chromatography. The raw solution containing 0.1-20% hemoglobin. At the first stage, anion-exchanging column is used when the admixtures are more acidic than hemoglobin, or cation-exchanging column is used when the admixtures are more alkaline than hemoglobin. The continuous flow of the solution results first in the complete saturation of the column with hemoglobin and the admixtures with high affinity to the column, then the column is overloaded following the substitution of hemoglobin. At the second stage, the ion-exchanger alternative to that used at the first stage is employed. Again, upon the complete saturation and overloading of the column, hemoglobin is substituted.
UA95058439A 1993-09-21 1994-09-20 Method for purifying hemoglobin from raw solution UA35599C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA002106612A CA2106612C (en) 1993-09-21 1993-09-21 Displacement chromatography process
PCT/CA1994/000514 WO1995008574A1 (en) 1993-09-21 1994-09-20 Displacement chromatography process and purified hemoglobin product

Publications (1)

Publication Number Publication Date
UA35599C2 true UA35599C2 (en) 2001-04-16

Family

ID=4152343

Family Applications (1)

Application Number Title Priority Date Filing Date
UA95058439A UA35599C2 (en) 1993-09-21 1994-09-20 Method for purifying hemoglobin from raw solution

Country Status (9)

Country Link
JP (1) JP4057049B2 (en)
KR (1) KR100361894B1 (en)
AT (1) ATE175975T1 (en)
DE (1) DE69416103T2 (en)
DK (1) DK0668877T3 (en)
ES (1) ES2126775T3 (en)
HU (1) HU218159B (en)
RU (1) RU2145873C1 (en)
UA (1) UA35599C2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2439875B (en) * 2005-04-22 2010-10-20 Waters Investments Ltd Apparatus and methods for mass spectrometric directed purification of biopolymers
NZ597809A (en) 2009-08-06 2014-05-30 Genentech Inc Method to improve virus removal in protein purification
EP2640483A1 (en) * 2010-11-15 2013-09-25 Biogen Idec Inc. Enrichment and concentration of select product isoforms by overloaded bind and elute chromatography
TR201815709T4 (en) 2011-12-22 2018-11-21 Hoffmann La Roche Ion exchange membrane chromatography.
EP2994485B1 (en) * 2013-05-06 2018-01-10 Sanofi Continuous multistep process for purifying antibodies
TW202128264A (en) * 2019-09-25 2021-08-01 瑞士商赫孚孟拉羅股份公司 Cation chromatography using predicted elution buffer salt concentration
CN114712894B (en) * 2022-04-08 2024-04-30 无锡博慧斯生物医药科技有限公司 Activating solution, activating method and application of glycosylated hemoglobin chromatographic column

Also Published As

Publication number Publication date
ES2126775T3 (en) 1999-04-01
DE69416103D1 (en) 1999-03-04
DK0668877T3 (en) 1999-09-13
JPH08503714A (en) 1996-04-23
ATE175975T1 (en) 1999-02-15
RU2145873C1 (en) 2000-02-27
HUT72924A (en) 1996-06-28
RU95112503A (en) 1996-12-20
DE69416103T2 (en) 1999-08-05
HU9501479D0 (en) 1995-07-28
HU218159B (en) 2000-06-28
JP4057049B2 (en) 2008-03-05
KR100361894B1 (en) 2003-05-12

Similar Documents

Publication Publication Date Title
GR3032728T3 (en) Purification of alpha-1 proteinase inhibitor using novel chromatographic separation conditions
ZA200005879B (en) Protein purification by ion exchange chromatography.
MX9707909A (en) Antibody purification by low-ph hydrophobic interaction chromatoggraphy.
FR2603581A1 (en) PROCESS FOR ISOLATING AND PURIFYING AMINO ACIDS BY CHROMATOGRAPHY
SE9603068D0 (en) Process for purifying a protein
ES8501979A1 (en) Highly purified human antihemophilic factor and method for the preparation thereof.
ATE338059T1 (en) PURIFICATION OF ECHINOCANDIN CYCLOPEPTIDES
UA35599C2 (en) Method for purifying hemoglobin from raw solution
MX9709312A (en) Method for purifying thrombopoietin.
CA2094347A1 (en) A method of producing a factor viii preparation
IL87543A0 (en) Interleukin 1 inhibitors,methods for the purification thereof and pharmaceutical compositions containing the same
EP0373540A3 (en) Method for purifying fibroblast growth factor protein
GR3006419T3 (en)
Hansson et al. Adsorption and desorption of proteins in metal chelate affinity chromatography: purification of albumin
BG102663A (en) Method for the purification of thrombino-like proteases from snake poison
MD726G2 (en) Process for purification of waste waters from the dyes
JPS557006A (en) Purification of urokinase
Redaelli et al. Evaluation of Coumarins and Dicycloethers in Matricaria chamomilla Flowers and Chamomile Extracts by Reverse-Phase HPLC
Yoder et al. An improved bulk purification method for Escherichia coli elongation factor, Ts
ES2072184A1 (en) Protein Petroglaucin 1 from the plant Petrocoptis glaucifolia (Lag.) Boiss, process for obtaining it and its use
KR970009339B1 (en) Tpa from scolopendra subepinpes multilans and its purification method
RU93054542A (en) METHOD FOR DETERMINING GLICKED HEMOGLOBIN IN BLOOD
RU93054567A (en) SORBENT BASED ON SILICA
MY102245A (en) Purification of antibodies
KR970070017A (en) Method for purifying recombinant granulocyte colony stimulating factor (rhG-CSF)