UA155130U - METHOD OF CRYOPRESERVATION OF HUMAN UMBILICAL CORD FRAGMENTS - Google Patents
METHOD OF CRYOPRESERVATION OF HUMAN UMBILICAL CORD FRAGMENTS Download PDFInfo
- Publication number
- UA155130U UA155130U UAU202303896U UAU202303896U UA155130U UA 155130 U UA155130 U UA 155130U UA U202303896 U UAU202303896 U UA U202303896U UA U202303896 U UAU202303896 U UA U202303896U UA 155130 U UA155130 U UA 155130U
- Authority
- UA
- Ukraine
- Prior art keywords
- cryopreservation
- umbilical cord
- human umbilical
- cord fragments
- rheopolyglucin
- Prior art date
Links
- 238000005138 cryopreservation Methods 0.000 title abstract 3
- 239000012634 fragment Substances 0.000 title abstract 3
- 210000003954 umbilical cord Anatomy 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 abstract 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 abstract 2
- 239000002577 cryoprotective agent Substances 0.000 abstract 2
- 239000002609 medium Substances 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 235000015097 nutrients Nutrition 0.000 abstract 2
- 241000283690 Bos taurus Species 0.000 abstract 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 abstract 1
- 238000004113 cell culture Methods 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 239000012091 fetal bovine serum Substances 0.000 abstract 1
- 230000001605 fetal effect Effects 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Спосіб кріоконсервування фрагментів пуповини людини передбачає розміщення фрагментів пуповини у суміші поживного середовища (для культивування клітин) та кріопротектора з подальшим поступовим охолодженням одержаної суміші до температури її замороження. Як кріопротектор використовують розчин диметилсульфоксиду в реополіглюкіні. Як поживне середовище використовують DMEM/F12 та бичачу фетальну сироватку. Співвідношення розчину диметилсульфоксиду в реополіглюкіні, DMEM/F12 та бичачої фетальної сироватки складає, мас. %: 20:54:26. Перед кріоконсервацією цільового продукту, яку виконують при -196 C, здійснюють поступове зниження температури до -80 CC.The method of cryopreservation of human umbilical cord fragments involves placing the umbilical cord fragments in a mixture of nutrient medium (for cell cultivation) and cryoprotectant followed by gradual cooling of the obtained mixture to its freezing temperature. A solution of dimethylsulfoxide in rheopolyglucin is used as a cryoprotectant. DMEM/F12 and fetal bovine serum are used as a nutrient medium. The ratio of the solution of dimethylsulfoxide in rheopolyglucin, DMEM/F12 and bovine fetal serum is, by weight. %: 20:54:26. Before cryopreservation of the target product, which is performed at -196 C, a gradual temperature decrease to -80 C is carried out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAU202303896U UA155130U (en) | 2023-08-15 | 2023-08-15 | METHOD OF CRYOPRESERVATION OF HUMAN UMBILICAL CORD FRAGMENTS |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAU202303896U UA155130U (en) | 2023-08-15 | 2023-08-15 | METHOD OF CRYOPRESERVATION OF HUMAN UMBILICAL CORD FRAGMENTS |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA155130U true UA155130U (en) | 2024-01-17 |
Family
ID=89511433
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UAU202303896U UA155130U (en) | 2023-08-15 | 2023-08-15 | METHOD OF CRYOPRESERVATION OF HUMAN UMBILICAL CORD FRAGMENTS |
Country Status (1)
| Country | Link |
|---|---|
| UA (1) | UA155130U (en) |
-
2023
- 2023-08-15 UA UAU202303896U patent/UA155130U/en unknown
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