UA154720U - METHOD OF OBTAINING FIBRINO(GENO)LYTIC ENZYME FROM SEA COMB TISSUE EXTRACT - Google Patents
METHOD OF OBTAINING FIBRINO(GENO)LYTIC ENZYME FROM SEA COMB TISSUE EXTRACT Download PDFInfo
- Publication number
- UA154720U UA154720U UAU202106490U UAU202106490U UA154720U UA 154720 U UA154720 U UA 154720U UA U202106490 U UAU202106490 U UA U202106490U UA U202106490 U UAU202106490 U UA U202106490U UA 154720 U UA154720 U UA 154720U
- Authority
- UA
- Ukraine
- Prior art keywords
- fibrino
- geno
- lytic enzyme
- tissue extract
- obtaining
- Prior art date
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- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 238000004587 chromatography analysis Methods 0.000 abstract 3
- 241000237510 Placopecten magellanicus Species 0.000 abstract 2
- 229920002684 Sepharose Polymers 0.000 abstract 2
- 239000002594 sorbent Substances 0.000 abstract 2
- 241000370939 Adamussium colbecki Species 0.000 abstract 1
- 239000012505 Superdex™ Substances 0.000 abstract 1
- 238000001042 affinity chromatography Methods 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
Abstract
Спосіб отримання фібрино(гено)літичного ферменту з екстракту тканин морського гребінця, за яким отримують екстракт тканин джерела фібрино(гено)літичного ферменту в буферному розчині, центрифугують, проводять тристадійну хроматографію, збирають фракції, які виявляють цільову активність, потім їх ліофілізують. Джерелом фібрино(гено)літичного ферменту служить екстракт тканин морського гребінця антарктичного Adamussium colbecki, при цьому тристадійна хроматографія включає афінну хроматографію, яка поєднує етапи із застосуванням сорбентів SBTI-sepharose і Blue-sepharose, та хроматографію, що поділяє за розмірами, на сорбенті Superdex 200 PG.The method of obtaining fibrino(geno)lytic enzyme from sea scallop tissue extract, according to which the tissue extract of the source of fibrino(geno)lytic enzyme is obtained in a buffer solution, centrifuged, three-stage chromatography is performed, fractions that show the target activity are collected, then they are lyophilized. The source of the fibrino(geno)lytic enzyme is an extract of the tissues of the Antarctic sea scallop Adamussium colbecki, while the three-step chromatography includes affinity chromatography, which combines the steps using SBTI-sepharose and Blue-sepharose sorbents, and size-separation chromatography on Superdex 200 sorbent PG.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAU202106490U UA154720U (en) | 2021-11-17 | 2021-11-17 | METHOD OF OBTAINING FIBRINO(GENO)LYTIC ENZYME FROM SEA COMB TISSUE EXTRACT |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAU202106490U UA154720U (en) | 2021-11-17 | 2021-11-17 | METHOD OF OBTAINING FIBRINO(GENO)LYTIC ENZYME FROM SEA COMB TISSUE EXTRACT |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA154720U true UA154720U (en) | 2023-12-13 |
Family
ID=89076498
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UAU202106490U UA154720U (en) | 2021-11-17 | 2021-11-17 | METHOD OF OBTAINING FIBRINO(GENO)LYTIC ENZYME FROM SEA COMB TISSUE EXTRACT |
Country Status (1)
| Country | Link |
|---|---|
| UA (1) | UA154720U (en) |
-
2021
- 2021-11-17 UA UAU202106490U patent/UA154720U/en unknown
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