UA147274U - METHOD OF DISINFECTION OF ANIMAL PREMISES IN VIRAL DISEASES - Google Patents

Abstract

Method of disinfection of livestock premises, surfaces, vehicles and other objects and equipment subject to veterinary supervision in case of viral diseases, in which 0.8% solution of DesSan in the form of aerosol is sprayed at the rate of 5 ml of solution per 1 m3 of premises. The product contains glutaraldehyde, dodecyldimethylammonium chloride, alkyldimethylbenzylammonium chloride, octyldecyldimethylammonium chloride, dioctyldimethylammonium chloride, auxiliary components (ethanol, glutamic acid, highly purified water).

Description

A useful model belongs to veterinary medicine and can be used to destroy RNA

DNA-containing viruses in livestock and poultry premises, on surfaces, in vehicles and other objects and equipment that are subject to veterinary supervision.

For this purpose, Aldecol DEZ, a disinfection solution, is officially registered and used in Ukraine - the manufacturer is Evabo Hemikalien GmbH, Germany. The tool contains three ADRs (active substances): glutaraldehyde - 12.5 90; benzalkonium chloride - 5.0 95 and formaldehyde - 9.0 vol.

The disadvantage of this agent is a sufficiently high antiviral effective concentration of the disinfectant for 0.595 at exposure of 120 minutes. Also, the disinfectant contains formaldehyde in its composition, which poses a potential and real threat to human health and the sanitary and ecological condition of livestock facilities and the environment, and is an environmentally dangerous and toxic agent that affects the nervous system, parenchymal organs and is a carcinogen (|Rizspeg M.N. Te iyuhis epPesibv oi Toptaidenude apa yogtaiy. Te uoshtai! oi ehreptenpia! tedisipe. - 1905. - 6 (4-6). - 487-518. pprz //doii.oga/10.1084/et. 6.4-6.487. Spip-Nviao Tvepud. A Vemiem op TNiagoiidipediopez ap Viadadeg Sapseg ip Nytap bshaiev5. docstai! oi

Epmigoptepia! bsiepsee apa Neay, Rap S. - 2014. - 32. - 1. - 1-45

The well-known product Virosan - a solution for disinfection - is produced by Bio-Test-Laboratory LLC.

Ukraine. The tool contains two ADRs (95): glutaraldehyde - 11.0 and benzalkonium chloride - 25.0.

The disadvantage of this product is the presence in the composition of only one cationic surface-active substance - benzalkonium chloride. It shows a low antimicrobial effect, which decreases in the presence of methylcellulose, polyvinyl and fats, which limits the range of use of this disinfectant. |Pnpotavz H.., Vyvzei A.O., Mayaga .-m. Apiitisgobia! asiimpu oi spiotechikaie diaseia!ee apa 30 repgaiKopiyit sPiogide adaipobi Rzattotav aegidiposa apa her ge5ropze 00 Bioside geziviapsev // U. Arri. Mystobrioi - 2005. - Mo 98. - R. 533-543; Shkarin V.V., Saperkin N.V.,

Kovalisheva O.V. et al. Regional monitoring of resistance of microorganisms to disinfectants: results and prospects // Medical almanac. - N. Novgorod, 2012. - Mo Z (22). - b. 122-125; Paly A.P., Zavgorodny A.I. Bactericidal properties of disinfectants from groups! Quaternary 35 ammonium compounds relative to mycobacteria // Herald of Altai State. Univ. - 2013. - Mo 6. - P. 79-81.)

Co.) The well-known agent DESOLINE-F, a solution for disinfection - manufacturer JSC Vetbiochem, Russia

Federation. The tool contains three ADRs (95): glutaraldehyde -7.5; benzalkonium chloride - 5.0; formaldehyde - 7.5.

The product contains formaldehyde, a toxic and carcinogenic substance, and the instructions for using the disinfectant recommend its use in the presence of birds in the poultry house - spray 0.4 95% solution once a week on the ceiling. This creates danger for birds and service personnel (R.-A. Ritsep, A. Ktateg, K. Nettapp, U. Vgeteg, 5. Kosy.

EoptaIdenude Meshygoyuhisiu ip Apita! Ehreptepiv Raipoiodu-Nezeagsi apa Rgasiise. - 2000. - 196. - 3. - 193-198.)

The closest analogue to the proposed useful model is Gypsy 8 (СРО 8), a solution for disinfection, manufactured by Evans Vanolin International, Great Britain. It is a liquid containing two ADRs in the total amount of 16 9o, namely: glutaraldehyde - 12.0 and didecyldimethylammonium chloride - 4.095. The named agent is traditionally used for disinfection of livestock and poultry premises, slaughterhouses and meat processing plants, vehicles and other facilities and equipment that are subject to veterinary supervision.

Certificate of state registration of the disinfectant No. AV-01550-03-10 dated October 28, 2010. I. But the analysis of the instructions for the use of Gypsy and practical observations indicate that this agent has one quaternary ammonium compound (didecyldimethylammonium chloride) in its composition.

It has a limited spectrum of action: it does not affect spores and enveloped viruses. To obtain the effect, high concentrations and a long period of action are required.

The basis of a useful model is the task of creating a method of disinfecting livestock premises in case of viral diseases by using a tool in which three different TIMES are additionally introduced and the total number of ADRs is increased to 22 95 (which is more than 37.5 95), which creates a highly effective tool for RNA- and DNA-containing viruses for preventive, current, final and forced disinfection of livestock and poultry premises.

The task is solved by the fact that in the method of disinfection of livestock premises, surfaces, vehicles and other objects and equipment that are subject to veterinary supervision in the case of viral diseases, according to a useful model, a 0.8 95 solution of DezSan is sprayed in the form of an aerosol, with calculation of 5 ml of solution per 1 m" of the room, which contains glutaraldehyde 9.5-11.0; dodecyldimethylammonium chloride 2.0-2.5; 60 alkyldimethylbenzylammonium chloride 4.5-5.0; octyldecyldimethylammonium chloride 3.0-4 ,0;

dioctyldimethylammonium chloride 1.0-2.0; auxiliary components (ethanol, glutamic acid, highly purified water) the rest.

The virucidal effect of DezSan on the Aujeski disease virus (Study of the physical and chemical properties of combined disinfectants / V.L. Kovalenko, M.F. Yashchenko, A.I. Chehun, E.V.

Rezunenko // Problems of zooengineering and veterinary medicine. - Kharkiv State Veterinary Academy. - 2009. - Mo 19, Part 2. - Vol. 3. - P. 195-199).

Disinfectant effect on the virus of the tested agent (disinfectant) is expressed in the absence of cytopathic action in the cell culture monolayer in certain dilutions of the disinfectant. To determine the degree of antiviral action of the DezSan disinfectant, the production strain "UNDIEV 18 V" - Aujesky's disease virus (VHA) was used, using different concentrations of the disinfectant. A transplant culture of cells with known characteristics and stable during at least three consecutive SNEV passages was used as a test object.

Control of virus cultures was carried out by twice-daily observation with microscopy of the suspension from the corresponding control and experimental wells (Table 1).

They determined not only the viability of the cell culture, but also the degree of cell proliferation after the effect of the studied disinfectant on them. The cell culture was observed using microscopy from 1 to 6 days before the end of the experiment, and the number of cells in the wells of the microplate was counted from 4 to 6 days inclusive. DezSan with a concentration of 1.0 95 on SPEV cell culture caused a slight cytopathic effect, so these concentrations were not evaluated in relation to the effect of disinfectants on Aujeski's disease virus. It should be noted that as a result of the studies of toxic effects and proliferative changes in SNEV cell culture, no evidence was found. This testifies to the effectiveness and safety of the disinfectant against the virus up to 0.5 95 concentration.

Table 1

Inactivation of the Aujesky disease virus under the action of a disinfectant (suspension method) DezSan, 95, (Met, p-6) 1 05255015 1 0585045" o 8000 joavyuvu | vvtyuvn 0/0 108.5620.39 o o nstKi uch PO U PO NO 106450, 65 0 0 0 catchable | 0

Note: the initial titer of Aujeski's disease virus is 10255EID2/cm3; "In the numerator, the residual infectivity of the virus is indicated in Id EiDvo/cm3U;

In the denominator is the efficiency of virus inactivation, 905.

PH (neutralization reaction) was used to confirm the cytopathic effect. Based on the data in the table. 1, it can be concluded that 0.1 95 solution of DezSan disinfectant after 15 minutes inactivates the virus not completely, but only by 44.28; after 30 minutes at the same concentration, DezSan inactivates the virus by 90 95, and after 1 hour. - at 97 95. Under the action of 0.25 95 DezSan solution after 15 minutes

99.79 95 of Aujeski's disease virus is destroyed, and after 30 minutes in 1 hour. the agent in the same concentration completely inactivates the virus. It should be noted that with a concentration of 0.5 to 95 DezSan has pronounced virucidal activity and can completely inactivate the Aujeski disease virus within 15, 30 and 60 minutes. After analyzing the data, it can be stated that 0.25 95 disinfectant solution

DezSan completely kills the Aujeski disease virus after 30 minutes, and starting with 0.5 95 concentration - after 15 minutes.

The virucidal effect of DezSan on Techen disease virus. To determine the effectiveness of the virucidal concentration of "Bi-des "M" against Teshen's disease virus (Tezspomigiv), the production strain "BUCHACH", a suspension of virus-containing material was used, which was obtained after the multiplication of the virus on SNEV cell cultures at the level of three passages. Control of virus cultures was carried out by twice-daily observation with microscopy of the suspension from the corresponding control and experimental wells. DezSan agent with a concentration of 0.1 95 on SNEV cell culture caused a slight cytopathic effect. Also, as a result of the conducted studies, it can be noted that the disinfectant did not lead to proliferative changes in the cell culture

SNEV up to 0.5 95 (Table 2).

Table 2

Inactivation of Techen disease virus under the action of DezSan disinfectant (suspension method), 95, Met, p-b6 le 1 09302018 1 06.8х0.53 o 15 45.6750.357 99.56-0.3877 100 30 108.620.62 o o 92.00530.427x 100 100 107220.42 o o 95.0050.7 375 100 100

The initial titer of Teshen's disease virus is 1093 EID5o/cm3; "In the numerator, the residual infectivity of the virus is indicated in IdEiDvo/cm3; "In the denominator, the effectiveness of virus inactivation, 95.

In the role of a test system, SNEV cells were used, in which the Teschen disease virus was multiplying. Based on the data in the table. 2, it can be concluded that after 15 minutes 0.1 95 solution of DezSan disinfectant inactivates 45.76 95 virus particles; after 30 minutes, neutralization of the virus occurs at 92 95, and after 1 hour. - at 9595. DezSan solution at a concentration of 0.25 95 after 15 minutes destroys 99.56 95 of the virus, and after 30 minutes and 1 hour. the tool completely inactivates the virus. Thus, DezSan in a concentration of 0.5 95 has pronounced virucidal activity and is able to completely destroy the virus of Teshen's disease within 15, 30, 60 minutes. As a result of the conducted research, it was established that the means in concentrations of 0.25 and 0.5 95 do not affect the change of the culture monolayer, therefore, they can be recommended for carrying out disinfection for the purpose of preventing viral diseases.

The study of the disinfection activity of DezSan was carried out on two model viruses, namely, the model of the vaccine strain of poliovirus type 1 (a simple RNA-containing virus) and the model of the strain of adenovirus type 5 (a simple DNA-containing virus). Both viruses are highly resistant to the action of physical and chemical environmental factors. Poliovirus type 1 was used at a working concentration of 105 TDC5»o/ml and cultivated on transplantable cell culture

NEr-2. Adenovirus type 5 was used at a working concentration of 109 TDC5BO/ML and was also cultivated on a transplantation culture of HEr-2 cells. Research was carried out according to

Methodological recommendations "Determining the virucidal activity of disinfectants", approved by the order of the Ministry of Health of Ukraine No. 231 dated 04/08/2009 and EM 14476. The virucidal activity of DezSan in the disinfection of vaccine poliovirus type 1 on batiste test objects is presented in Table 3.

Table From the experiment by means (96)

YAN NN | axis 1 an JANV | os- «|i -- || nt nt : I |- nt with se || nt

YAN YANV | ос-ш ши: ин п В 1 ши: нин п V ши: нин п V ши: нин п B 2 0.25 95 ши: нин п V ши: нин п V ши: нин п В

From shi: nin p In shi: in sha B 111111-- 1 111111-- 111111-- ool-- zh 00000100 2 0.8 de 111111-- 111111-- 111111-- I I who p B s YOU a Y « 0000000 r pi V s V a Y

NN - the presence of cytopathogenic effect of poliovirus ----------- absence of cytopathogenic effect of poliovirus - the study was not conducted

KZTO control of contamination of test objects with polioviruses

Livelihood control of the viability of polioviruses

KKT control of Ner-2 cell culture

Thus, 0.8 95 concentration of DezSan leads to the inactivation of poliomyelitis type 1 virus after 30 minutes; 0.25 95 concentration of the agent leads to inactivation of poliomyelitis virus type 1 after 60 minutes, and 0.195 concentration of the agent leads to inactivation of poliomyelitis virus type 1 after 120 minutes on batiste test subjects. Accordingly, DezSan will have an antiviral effect against less resistant viruses, in particular - influenza, viral hepatitis, adenoviruses, rotavirus gastroenteritis, coronaviruses, including - the BAN5Z-Soh-2 virus that causes SOMIO-19 and others. The virucidal activity of DezSan when disinfecting adenovirus type 5 on batist test objects is presented in Table 4.

Table 4 of the experiment by means (96)

WITH YANNY | NN | o--- 1 S YANNYU | NN | at--

WITH YANNY | NN | at--

WITH YANNY | NN | o-- 2 0.1 ze S YANNYU | NN | at--

WITH YANNY | NN | at--

WITH YANNY | NN | at--

With S YANNY | NN | at--

S YANNY UNO | oh

WITH YANNY | sun sia 1 JAN | sleep

YAN | c- | everything

YAN | c- | se 2 0.25 95 JAN | c- | everything

YAN | c- | everything

YAN | c- | everything

With JAN | c- | everything

YAN | s-' 01000 p V VY a Y 1 p Vs VY a Y p Vs VY a Y p Vs VY a Y 2 0.8 where p Vs VY a Y p Vs VY a Y p Vs VY a AND

Z p V s VI a Y p V s NI o V

Yukv kzto p V VI a Y ono B-- 5 - p V V a V

NN - presence of cytopathogenic effect of poliovirus -----------absence of cytopathogenic effect of poliovirus - no study was conducted

KZTO control of contamination of test objects with polioviruses

Livelihood control of the viability of polioviruses

KKT control of Ner-2 cell culture

Thus, 0.8 95 concentration of the agent in DezSan leads to the inactivation of adenovirus type 5 after 30 minutes; 0.25 95 concentration of the agent results in inactivation of adenovirus type 5 after 60 minutes, and 0.1 95 concentration of the agent results in inactivation of adenovirus type 5 after 120 minutes on batiste test objects.

Therefore, the study of the virucidal activity of DezSan showed the presence of such an action as against

against RNA-containing and DNA-containing viruses, detection of antiviral activity against less resistant viruses, in particular - influenza, viral hepatitis Anoro-, adenoviruses, rotavirus gastroenteritis, coronaviruses, including - BANO-Soh-2 virus, which causes SOMIO -19 and others.

Claims (1)

USEFUL MODEL FORMULA A method of disinfection of livestock premises, surfaces, vehicles and other objects and equipment that are subject to veterinary supervision in the case of viral diseases, which is characterized by the fact that 0.895 solution of DezSan is sprayed in the form of an aerosol, at the rate of 5 ml of solution per 1 m a room containing glutaraldehyde 9.5-11.0; dodecyldimethylammonium chloride 2.0-2.5; alkyldimethylbenzylammonium chloride /-4.5-5.0; octyldecyldimethylammonium chloride 3.0-4.0; dioctyldimethylammonium chloride 1.0-2.0; auxiliary components (ethanol, glutamic acid, highly purified water) the rest.