UA140771U - METHOD FOR REDUCING EMBRYOTOXICITY OF CADMIUM CHLORIDE IN ANIMALS - Google Patents

Abstract

A method of reducing the embryotoxicity of cadmium chloride, which uses detoxifying complexing compounds. Selenium citrate is used as a detoxification compound against the background of cadmium chloride intoxication, the solution of which is administered intragastrically at the rate of 0.1 mg / kg of animal body weight once a day for 19 days of pregnancy of the female, followed by a conclusion on detoxification efficacy.

Description

A useful model belongs to medicine, namely to experimental morphology and anatomy, and can be used in the study of the influence of toxic factors on the body, in particular heavy metal salts, on the course of embryogenesis and the reproductive system.

The method of detoxification of the body in the case of poisoning with ethanol and heavy metal salts is closest in terms of technical essence and the result achieved (I.Ya. Demkiv, I.M. Klishch. The effect of the enterosorbent "Algigel" on the biochemical parameters of the body of white rats poisoned with ethyl alcohol against the background of chronic intoxication by salts of lead and cadmium: Collection of abstracts of the International scientific conference of students and post-graduate students "Youth and progress in biology", - L.: Lviv National University named after I. Franko. - 2008. - 420 p.), which includes the use of detoxifying complex-forming compounds. According to a known method, resorcilaldehyde semicarboside (2,4-dihydroxybenzaldehyde semicarboside - С8НОМЗ3О3) is used as a complexing compound, which ensures quick and effective mobilization of compensatory mechanisms in the cells of the affected liver.

The disadvantage of the known method is insufficient detoxification efficiency, which follows from the fact that the use of resorcilaldehyde semicarbozide does not ensure the inactivation of reactive oxygen species. The disadvantages include the inability of resorcilaldehyde hemicarboside to reduce the embryotoxicity of cadmium chloride and to have a compensatory effect on the state of the reproductive system in case of cadmium intoxication.

The basis of a useful model is the task of finding a new factor aimed at reducing the embryotoxic effect of cadmium chloride and restoring the functional state of the reproductive system (ovaries, placentas) of female animals.

The problem is solved by the fact that in the method of reducing the embryotoxicity of cadmium chloride, which includes the use of detoxification complexing compounds, according to a useful model, selenium citrate is used as a detoxification compound against the background of cadmium chloride intoxication, the solution of which is administered intragastrically at the rate of 0.1 mg/kg of weight the body of the animal once a day during the 19 days of the female's pregnancy, followed by a conclusion on detoxification efficiency.

Microelements in the form of carboxylates of food acids, primarily in the form of citrates, are a natural defense system against many toxicants. Organic and complex forming

Germanium compounds have immunomodulatory, antitumor, antiviral, neuro-, cardio-, hepatoprotective, detoxifying, and membrane-protective effects. In addition, germanium compounds have antihypoxic and antioxidant activity, which is essential during pregnancy and lactation, growth, development, and normal life of the body.

Selenium citrates have a pronounced radioprotective effect in relation to the fetus, reduce the frequency of stillbirths, potentiate the effects of iodine in the process of organogenesis during antenatal development; act as an inhibitor of apoptosis, have anti-ischemic properties, an anti-carcinogenic effect, are able to reduce the frequency of radiation-induced tumors in the long term, which makes it expedient to use metal citrates to inhibit the toxic effects of cadmium compounds.

The method is carried out as follows. A laboratory animal - a white female rat that has become pregnant - is simulated with cadmium chloride poisoning pre-intragastrically at a dose of 1.0 mg/kg - for 19 days. Against the background of intoxication, a solution of selenium citrate is also administered intragastrically at a dose of 0.1 mg/kg of the animal's body weight once a day during the 19 days of the female's pregnancy. The conclusion about the effectiveness of detoxification is made based on morphological indicators: fertility index, ZES, PIS, PostiS, the number of fetuses per 1 female and the number of corpora lutea of pregnancy.

Table 1

Results of the embryotoxicity analysis of selenium citrate index of fertility 08 | 7777/7097 | 777708 2

Number of animals (females) | 77/78 17777778 11111181

Number of live fruits. 7777771 Ї7111731711111160111111Ї1111175СС

Table 1

Results of embryotoxicity analysis

Research groups

Indicator Control. Cadmium chloride p Cadmium chloride rhyd cad selenium citrate

The number of corpora lutea of pregnancy on and so 9.6050.40 10.25:30.27 9.91-1.067; a female

Body weight of 1 fetus, g 2.38520.08 2.2150.17 2.28g0.04

Placenta mass, mg 0.5920.02 0.57-0.02 0.58:0.02

Placenta size, mm 1.51-40.04 1.4430.03 1.4920.02

Total embryonic mortality, 90 7.1253.43 23.17-1.43 10.2153.22222

Preimplantation mortality, one 0.2350.06 0.14x0.10

Post-implantation mortality, unit 0.01:-0.01 0.10-0.06 0.02:20.01

Note: 7 - p«e0.05; и - r«k0.01; and - p«0.001; 7 - p-0.05-0.1 in relation to the control; 22 -r«0.05; 777 - p 0.001; - p-0.05-0.1 in relation to the group with cadmium chloride

Example 1. At the beginning of the experiment, female rats with a dated pregnancy were obtained, for which the estrous cycle was studied, they were mated with males, and the first day of pregnancy was determined based on the presence of spermatozoa in vaginal smears. White rats - pregnant females, weighing from 170 g were simulated lesions with cadmium chloride, for which an aqueous solution of cadmium chloride was administered intragastrically at the rate of 1.0 mg/kg - daily, for 19 days. At the same time, selenium citrate obtained by nanoaquatechnology at the rate of 0.1 mg/kg of the animal's body weight was administered once a day during 19 days of pregnancy. After that, on the 20th day of the experiment, embryos were surgically removed. Female ovaries were also removed to determine the number of corpora lutea of pregnancy. Embryos were weighed, measured, determined for their development at the normal stage of development, sex was determined and fixed for further histological studies. Detoxification efficiency was evaluated based on the dynamics of changes in the listed morphological indicators, which were calculated according to standard formulas. The results of the analysis were compared with similar indicators in the group of control animals and in the group exposed only to cadmium chloride at a dose of 1.0 mg/kg for 19 days. As a result of the study (see Table 1), a decrease in the embryotoxic effect of cadmium chloride was established in the group of combined exposure to cadmium and selenium citrate, which was indicated by an increase in the number and weight of embryos, as well as a decrease in embryonic mortality.

Claims (1)

USEFUL MODEL FORMULA A method of reducing the embryotoxicity of cadmium chloride, in which detoxification complexing compounds are used, which differs in that selenium citrate is used as a detoxification compound against the background of cadmium chloride intoxication, the solution of which is administered intragastrically at the rate of 0.1 mg/kg of the animal's body weight once per day during the 19 days of the female's pregnancy, followed by a conclusion on detoxification efficiency.