UA140098U - METHOD FOR PREDICTING THE DEVELOPMENT OF OSTEOARTHRITIS - Google Patents

Abstract

The method of predicting the development of osteoarthritis includes clinical examination, radiography. Additionally, in the patient's serum determine the polymorphism of the methylenetetrahydrofolate reductase gene (MTHFR C677T), the content of endothelin, folic acid, homocysteine, interleukin 6 (IL-6), glycosaminoglycans (GAG). With a homozygous carrier of 677-TT and levels of endothelin> 10 ng / ml, folic acid <6 ng / ml, homocysteine> 20 μmol / l, IL-6> 10 ng / l, GAG> 28 μmol / l predict the development of osteoarthritis.

Description

The proposed useful model The method of predicting the development of osteoarthritis belongs to medicine, in particular to orthopedics and rheumatology. It is prescribed and can be used in diagnosing the development of osteoarthritis in the early stages.

Methods of predicting the development of osteoarthritis are known. These include x-rays, studies of biopsies of the synovial membrane, synovial fluid, and cartilage biopsies (Okorokov

A.N. Diagnosis of diseases of internal organs. - M., 2001. - Vol. 2. - P. 186-196). The disadvantage of these methods is the lack of the possibility of predicting the dynamics of the process in the early subclinical stages, preventing its development and possible complications due to the internal characteristics of the body.

The basis of a useful model is the task of developing a method that would allow predicting the development of osteoarthritis in the early stages.

The task is solved by the fact that, in addition to clinical examination and radiography, the polymorphism of the methylenetetrahydrofolate reductase gene (MTNEV C677T), the content of endothelin, folic acid, homocysteine, interleukin 6 (IL-b), glycosaminoglycans (GAG) and in homozygous carriers are determined in the patient's blood serum 677-TT, endothelin content 210 ng/ml, folic acid 6 ng/ml, homocysteine 220 μmol/l, IL-b 510 ng/l, GAG 528 μmol/l predict the development of osteoarthritis.

Application of the method. During hospitalization, the patient is examined, X-rays are taken, and the MTNEV C677T gene polymorphism is determined. The content of endothelin, folic acid, IL-6, homocysteine, IL-6, GAG is determined by immunoenzymatic method. With homozygous carrier 677-177, levels of endothelin 210 ng/ml, folic acid "6 ng/ml, homocysteine 220 μmol/l, IL-b 510 ng/l, GAG "28 μmol/l predict the development of osteoarthritis.

A specific example of using the method

Patient L., 67 years old, was hospitalized in the clinic due to pain syndrome of the left hip joint. Examined, x-ray taken. No signs of osteoarthritis were found. Polymorphism of MTNEV C677T gene was determined in blood serum. The content of endothelin, folic acid, homocysteine, IL-6, GAG was determined by immunoenzymatic method. A homozygous carrier of 677-HT was established. Levels of endothelin - 23 ng/ml, folic acid - 3.5 ng/ml, homocysteine - 39 μmol/l, IL-6-20 ng/l, GAG - 47 μmol/l. Diagnosed development

From osteoarthritis. After 12 months, X-rays revealed signs of osteoarthritis.

Thus, the proposed method is informative and allows diagnosing the development of osteoarthritis at an early stage.

Claims (1)

USEFUL MODEL FORMULA The method of predicting the development of osteoarthritis, which includes a clinical examination, radiography, which is distinguished by the fact that, in addition, the polymorphism of the methylenetetrahydrofolate reductase gene (MTINEV Сб677Т), the content of endothelin, folic acid, homocysteine, interleukin 6 (IL-6), glycosaminoglycans (GAG) are determined in the patient's blood serum ) and with homozygous carrier 677- TI Her levels of endothelin 210 ng/ml, folic acid "6 ng/ml, homocysteine 220 μmol/l, IL-6 210 ng/l, GAG "28 μmol/l predict the development of osteoarthritis.