UA137771U - METHOD FOR PREDICTING THE DEVELOPMENT OF OSTEOARTHRITIS - Google Patents

Abstract

The method of predicting the development of osteoarthritis, including clinical examination, radiography, according to a useful model in addition to the serum of the patient determine the polymorphism of the nitric oxide synthase gene (eNOS T786C), the content of thrombomodulin, glycosaminoglycans (GAG 6), interg (6-) reactive protein (PSA), homocysteine and homozygous carrier 786-CC, thrombomodulin levels> 5 ng / ml, GAG> 28 μmol / l, IL-6> 10 ng / l, PSA> 6 mg / l, homocysteine> 20 μmol / l predict the development of osteoarthritis.

Description

A useful model belongs to medicine, in particular to orthopedics and rheumatology. It is prescribed and can be used in diagnosing the development of osteoarthritis in the early stages.

Methods of predicting the development of osteoarthritis were chosen as the closest analogue. These include radiography, examination of a biopsy of the synovial membrane, synovial fluid, and a cartilage biopsy (AN Okorokov. Diagnostics of diseases of internal organs. M., 2001. - vol. 2. - p. 186-196). The disadvantage of these methods is the lack of the possibility of predicting the dynamics of the process in the early subclinical stages, preventing its development and possible complications due to the internal characteristics of the body.

The basis of a useful model is the task of developing a method that would allow predicting the development of osteoarthritis in the early stages.

The task is solved by the fact that, in addition to clinical examination and radiography, the polymorphism of the nitric oxide synthase gene (emo5 1786C), the content of thrombomodulin, glycosaminoglycans (GAG), interleukin 6 (IL-6), C-reactive protein (CRP) is determined in the patient's blood serum ), homocysteine and with homozygous carrier 786-SS, levels of thrombomodulin »5 ng/ml, GAG »28 μmol/l, IL-b6 »10 ng/l, SRP »b mg/l, homocysteine 220 μmol/l predict the development of osteoarthritis .

Application of the method. Upon admission, the patient is examined, an X-ray is taken, the polymorphism of the eMmo5 T786C gene is determined, the content of thrombomodulin is determined by the immunoenzymatic method,

GAG, IL-6, SRP, homocysteine. With homozygous carrier 786-SS and levels of thrombomodulin »5 ng/ml, GAG »28 μmol/l, IL-6 »10 ng/l, SRP »6 mg/l, homocysteine 220 μmol/l predict the development of osteoarthritis.

A specific example of using the method.

Patient H., 65 years old, was admitted to the clinic due to pain in the left hip joint. Examined, x-ray taken. No signs of osteoarthritis were found. The eMMO5 T786C gene polymorphism and thrombomodulin, GAG, IL-6, SRP, and homocysteine content were determined in the serum. A homozygous carrier of 786-SS was established, thrombomodulin levels - 15 ng/ml, GAG - 37 μmol/l, IL-6-22 ng/l, SRP - 14 mg/l, homocysteine - 36 μmol/l. The development of osteoarthritis was diagnosed. After 12 months, X-rays revealed signs of osteoarthritis.

Thus, the proposed method is informative and allows diagnosing the development of osteoarthritis at an early stage.

Claims (1)

USEFUL MODEL FORMULA A method of predicting the development of osteoarthritis, which includes a clinical examination, radiography, which is distinguished by the fact that the polymorphism of the nitric oxide synthase gene (eMmOo5z 1T786C), the content of thrombomodulin, glycosaminoglycans (GAG), interleukin 6 (IL-6) is additionally determined in the patient's blood serum ), C-reactive protein (SRP), homocysteine and with homozygous carrier 786-SS, thrombomodulin levels »5 ng/ml, HAG »28 μmol/l, IL-6 »10 ng/l, SRP »6b mg/l, homocysteine 220 μmol/l predicts the development of osteoarthritis. 00 Computer keyboardG. Payalnikovo (00000000 Ministry of Economic Development, Trade and Agriculture of Ukraine, St. M. Hrushevskyi, 12/2, Kyiv, 01008, Ukraine SE "Ukrainian Institute of Intellectual Property", str. Glazunova, 1, Kyiv - 42, 01601