UA133406U - A METHOD FOR OBTAINING ASEPTIC CULTURE OF FRAGMENTS OF ORIENTAL OAK PLANTS (QUERCUS ROBUR L.) - Google Patents

Abstract

A method of obtaining an aseptic culture of fragments of shoots of plants of oak common (Quercus robur L.) involves sterilization of plant material under aseptic conditions using 70% ethyl alcohol for 1 min, washing with water, sterilization in a solution of NaClO sodium hypochloride, followed by 3-fold extraction water, in addition, sterilization of the fragments of shoots is carried out in 2.5% NaClO solution for 8-15 min followed by washing in sterile distilled water, and then kept in a solution of silver nitrate 1.0% AgN O3 for 8-15 minutes, and the shoot fragments are introduced into a hormonal nutrient medium according to Murasige and Skuga (MS) with the addition of 2 g / l of activated carbon.

Description

The useful model belongs to the field of agriculture, in particular - forestry.

Known as the closest analogue (Ip mio rgoradayop oi tehisap oak5 (Ocegsiv 5yr.) Loze zZimezige OeiIdadiPo-Oia? de Geop, dove Egapsivso MogaIez-Ootipdieg, Magia de! bosoto Zapiov5-

Oia?7, Eidepio Rege2-Moirpe-VaiYisni / Roiiiroiapisa. - 2013. - Me 35. - R. 85-97. - access mode: pEruLlimly tedaus.ogo/piti/621/62125675005/), which includes the selection of the method of sterilization of acorns

Opgetgsiv 5 years. and consists in washing them in tap water, holding them three times for 5 min in liquid soap (Oegtosieap 1 95 m/m), rinsing with tap water, immersion in 70 9o ethyl alcohol for 1 min, washing with water, sterilization in 20 96 commercial bleaching solution (5.25 95 sodium hypochlorite) for 25 min followed by washing three times in sterile distilled water under aseptic conditions.

The disadvantage of the above-mentioned method is its: 1) multi-stage and duration, which is not always acceptable in production conditions; 2) the sterilization regime by a known method ineffectively neutralized the exogenous microbiota of plant shoot fragments Otsetsgsiv Gorig H. (hereinafter - C). bitter).

The task of the useful model is to develop a method of obtaining aseptic fragments of plant shoots Opzegsyz gorig G. (hereinafter - C). gorig) in the ip myo culture to obtain in a short time a significant amount of sterile viable plant material 3 by further mass microclonal propagation.

To sterilize the fragments of the shoots of 0. gorig plants, the well-known method of sterilizing acorns was first used by Ocegsiv 5yr. As a result, a small number of aseptic explants were obtained (20 95 commercial bleaching solution ineffectively neutralized exogenous microbiota, on the 10th day of cultivation more than 60 95 explants were infected). In order to increase the percentage of asepticity of the plant material, in addition to the MasiO solution, 1.0 95 silver nitrate AYA9MO was also used."

The set task of a useful model is solved by the fact that the method of obtaining an aseptic culture of fragments of shoots of common oak plants (Oiyetsy5 goBygi.) which includes sterilization of plant material in aseptic conditions using 70 95 ethyl alcohol for 1 min, washing with water, sterilization in a solution of sodium hypochlorite MmasiyuO with after three-fold exposure in sterile distilled water in accordance with the proposed solution, shoot fragments are sterilized in a 2.5 95 solution of MaciO for 8-15 minutes with

This is followed by washing in sterile distilled water, after which they are additionally kept in a solution of silver nitrate 1.0 95 AdMOz for 8-15 min, and fragments of shoots are injected into a hormone-free nutrient medium according to the prescription of Murashige and Skoog (MS) with the addition of 2 g/l of activated coal.

The method of obtaining aseptic viable fragments of plant shoots 0). gorig, consists in using shoots 15-20 cm long from 50-100-year-old donor plants in the spring-summer period and allows to significantly reduce the duration of sterilization by eliminating operations of the preparatory stage in non-sterile conditions. To do this, in non-sterile conditions, the shoots are cut into fragments 3.0-5.0 cm long with a few buds using a scalpel. In laminar box conditions, they are immersed in 7095 ethyl alcohol for 1 minute, washed with water, sterilized in a 2.5 95 solution of MaciO for 8-15 minutes followed by washing in sterile distilled water, then sterilization is continued in a solution of 1.0 95 AdMOz for 8 -15 min, after which it is held three times in sterile distilled water. Then the plant material is cut into 1.0-1.5 cm explants with one bud and cultivated on a basic hormone-free nutrient medium MS with the addition of 2 g/l activated carbon.

The technical solution of the useful model makes it possible to obtain more than 8095 aseptic viable fragments of CO plant shoots). htobig with high regeneration capacity and significantly reduce the duration of sterilization.

Claims (1)

USEFUL MODEL FORMULA The method of obtaining an aseptic culture of fragments of shoots of common oak plants (Opyegsiv horiya 3), which includes sterilization of plant material in aseptic conditions using 70% ethyl alcohol for 1 min, washing with water, sterilization in a solution of sodium hypochloride MaSiO followed by 3-fold keeping in sterile distilled water, which differs in that the sterilization of shoot fragments is carried out in a 2.595 solution of MaciO for 8-15 minutes, followed by washing in sterile distilled water, after which they are additionally kept in a solution of silver nitrate 1.0 95 AdMOz for 8-15 minutes , and fragments of shoots are injected into a hormone-free nutrient medium according to the prescription of Murashige and Skoog (MS) with the addition of 2 g/l of activated carbon.