UA128793U - TEST-SYSTEM FOR DETECTION OF AFRICAN PIGS VIRUS DNA AFFECTED BY "Sui-DNA-test-ASF virus" Polymerase Chain Reaction - Google Patents
TEST-SYSTEM FOR DETECTION OF AFRICAN PIGS VIRUS DNA AFFECTED BY "Sui-DNA-test-ASF virus" Polymerase Chain ReactionInfo
- Publication number
- UA128793U UA128793U UAU201803284U UAU201803284U UA128793U UA 128793 U UA128793 U UA 128793U UA U201803284 U UAU201803284 U UA U201803284U UA U201803284 U UAU201803284 U UA U201803284U UA 128793 U UA128793 U UA 128793U
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- UA
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- dna
- virus
- solution
- sui
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Тест-система для виявлення ДНК вірусу АЧС за допомогою полімеразної ланцюгової реакції "Sui-DNA-test-ASF virus" включає два етапи: виділення нуклеїнових кислот з набором реактивів (набір № 1) та проведення ампліфікації з набором реактивів (набір № 2). Для виділення нуклеїнових кислот використовують набір реактивів (набір № 1), до складу якого входить лізуючий буфер та розчин для відмивки № 1 на основі гуанідину тіоціанату, розчин для відмивки № 3 на основі етанолу, розчин для фінальної відмивки на основі хлороформу та ізопропанолу, ТЕ-буфер, розчин сорбенту. Для проведення ампліфікації використовують набір реактивів (набір № 2), до складу якого входять розчини праймерів та зонду, розчин "RT-PCR MasterMix", деіонізована вода.The ASF virus DNA test system using the Sui-DNA-test-ASF virus polymerase chain reaction comprises two steps: isolation of nucleic acids with a set of reagents (set No. 1) and carrying out amplification with a set of reagents (set No. 2). For isolation of nucleic acids use a set of reagents (set No. 1), which includes lysis buffer and solution for washing No. 1 based on guanidine thiocyanate, solution for washing No. 3 based on ethanol, solution for final washing based on chloroform and isopropanol, TE - buffer, sorbent solution. For the amplification use a set of reagents (set number 2), which includes solutions of primers and probe, solution "RT-PCR MasterMix", deionized water.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
UAU201803284U UA128793U (en) | 2018-03-29 | 2018-03-29 | TEST-SYSTEM FOR DETECTION OF AFRICAN PIGS VIRUS DNA AFFECTED BY "Sui-DNA-test-ASF virus" Polymerase Chain Reaction |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
UAU201803284U UA128793U (en) | 2018-03-29 | 2018-03-29 | TEST-SYSTEM FOR DETECTION OF AFRICAN PIGS VIRUS DNA AFFECTED BY "Sui-DNA-test-ASF virus" Polymerase Chain Reaction |
Publications (1)
Publication Number | Publication Date |
---|---|
UA128793U true UA128793U (en) | 2018-10-10 |
Family
ID=63787287
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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UAU201803284U UA128793U (en) | 2018-03-29 | 2018-03-29 | TEST-SYSTEM FOR DETECTION OF AFRICAN PIGS VIRUS DNA AFFECTED BY "Sui-DNA-test-ASF virus" Polymerase Chain Reaction |
Country Status (1)
Country | Link |
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UA (1) | UA128793U (en) |
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2018
- 2018-03-29 UA UAU201803284U patent/UA128793U/en unknown
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