UA106926U - The method for determining the total number of non-sulfated glycosaminoglycans - Google Patents
The method for determining the total number of non-sulfated glycosaminoglycansInfo
- Publication number
- UA106926U UA106926U UAU201511544U UAU201511544U UA106926U UA 106926 U UA106926 U UA 106926U UA U201511544 U UAU201511544 U UA U201511544U UA U201511544 U UAU201511544 U UA U201511544U UA 106926 U UA106926 U UA 106926U
- Authority
- UA
- Ukraine
- Prior art keywords
- holes
- solution
- determining
- total number
- mcl
- Prior art date
Links
- 229920002683 Glycosaminoglycan Polymers 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 239000000243 solution Substances 0.000 abstract 3
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 abstract 1
- 239000012620 biological material Substances 0.000 abstract 1
- 238000011088 calibration curve Methods 0.000 abstract 1
- 239000012895 dilution Substances 0.000 abstract 1
- 238000010790 dilution Methods 0.000 abstract 1
- 229920002674 hyaluronan Polymers 0.000 abstract 1
- 229960003160 hyaluronic acid Drugs 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The method for determining the total number of non-sulfated glycosaminoglycans includes the preparation of solution of alcian blue, introducing of sample and solution into each cuvette, incubating and recording of colour reaction at 480 nm, creation of calibration curve. The analysis is performed in non-sterile plates, in the holes of which it is introduced per 20 mcl of test biological material. Simultaneously in appropriate calibration holes it is introduced highly purified hyaluronic acid in appropriate dilutions as standard. Then at the same time per 200 mcl of dye solution are added to all holes. It is performed the incubation for 15 minutes and with a microplate reader it is defined the optical density at a wavelength of 480-492 nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAU201511544U UA106926U (en) | 2015-11-23 | 2015-11-23 | The method for determining the total number of non-sulfated glycosaminoglycans |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAU201511544U UA106926U (en) | 2015-11-23 | 2015-11-23 | The method for determining the total number of non-sulfated glycosaminoglycans |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA106926U true UA106926U (en) | 2016-05-10 |
Family
ID=57142586
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UAU201511544U UA106926U (en) | 2015-11-23 | 2015-11-23 | The method for determining the total number of non-sulfated glycosaminoglycans |
Country Status (1)
| Country | Link |
|---|---|
| UA (1) | UA106926U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL247829B1 (en) * | 2023-06-23 | 2025-09-08 | Politechnika Bydgoska Im Jana I Jedrzeja Sniadeckich | Method for determining the concentration of sodium dodecyl sulfate in aqueous solutions |
-
2015
- 2015-11-23 UA UAU201511544U patent/UA106926U/en unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL247829B1 (en) * | 2023-06-23 | 2025-09-08 | Politechnika Bydgoska Im Jana I Jedrzeja Sniadeckich | Method for determining the concentration of sodium dodecyl sulfate in aqueous solutions |
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