TWM598730U - Mixing chip - Google Patents

Abstract

A hybrid chip is used to solve the problem of complicated use steps of the conventional hybrid chip. It includes: a first chip body having a first surface and a second surface opposite to each other, a plurality of injection grooves respectively penetrate through the first surface and the second surface, the first chip body having a plurality of first micro A runner, the first micro runner is connected to any two injection grooves, the second surface has at least one buffer groove; a second wafer body having a first surface and a second surface opposite to each other, the second wafer body The first surface is combined with the second surface of the first wafer body, the second wafer body has a collection groove pair located in one of the plurality of injection grooves, and a second micro channel connects the collection groove and the detection Slot; and a buffer flow path connecting the detection slot and the buffer slot.

Description

Hybrid chip

This creation is about a wafer for inspection, especially a hybrid wafer that can mix multiple fluids for inspection.

In the inspection process of biological and chemical test liquids, it is necessary to mix various liquids such as samples, test liquids or reaction liquids to form a test liquid, and then the test liquid is tested by the instrument. Generally speaking, the system After the above-mentioned liquids are sequentially poured into a container, they are mixed by vibrating or stirring to form the liquid to be measured, and the liquid to be measured is taken to the measuring position on the instrument before the instrument can perform the liquid to be measured. Detection, and the cumbersome preparation steps mentioned above will cause inconvenience in detection.

To this end, there is a conventional fluid mixing wafer. The fluid mixing wafer has a plurality of reagent tanks connected to a detection tank via a plurality of flow channels. According to this, the user separates the plurality of reaction solutions The reaction liquid and the sample are respectively injected into the plurality of reagent tanks, and the plurality of reaction liquids and samples are mixed in the detection tank through the plurality of flow channels to directly detect the fluid in the detection tank. However, in order to avoid overflow when the fluid converges into the detection tank, or to quantify the fluid in the detection tank, for example, when a spectrometer or other instrument is used to detect the absorption spectrum of the fluid in the detection tank, the system will usually The solution is set to 1ml. At this time, the user must control the amount of reaction solution and sample injected into the reagent tanks, which results in complicated steps and cannot effectively improve work efficiency.

In view of this, there is indeed a need to improve the conventional fluid mixing wafer.

In order to solve the above problems, the purpose of this creation is to provide a hybrid chip that can improve the inspection efficiency.

The directionality or similar terms used in the full text of this creation, such as "front", "rear", "left", "right", "up (top)", "down (bottom)", "inner", "outer" , "Side", etc., mainly refer to the direction of the attached drawings. The directional or similar terms are only used to help explain and understand the embodiments of this creation, and are not used to limit this creation.

The elements and components described in the full text of this creation use the quantifier "一" or "one" only for the convenience of use and to provide the usual meaning of the scope of this creation; in this creation, it should be interpreted as including one or at least one, and single The concept of also includes the plural, unless it clearly implies other meanings.

The approximate terms such as "combination", "combination" or "assembly" mentioned in the full text of this creation mainly include the types that can be separated without destroying the components after being connected, or the components can be made inseparable after being connected, and have common knowledge in the field Those can be selected based on the materials of the components to be connected or assembly requirements.

The hybrid chip of the present invention includes: a first chip body having a first surface and a second surface opposite to each other, a plurality of injection grooves respectively penetrate through the first surface and the second surface, and the first chip body has A plurality of first micro flow channels, the first micro flow channel is connected to any two injection grooves, the second surface has at least one buffer groove; a second wafer body has a first surface and a second surface opposite to each other, The first surface of the second wafer body is bonded to the second surface of the first wafer body, the second wafer body has a collection groove pair located in one of the plurality of injection grooves, and a second micro channel is connected to the A collection tank and the detection tank; and a buffer flow channel connects the detection tank and the buffer tank.

According to this, the hybrid chip of the present invention has the detection groove through the buffer flow channel through the second chip body. When the fluid is filled into the detection groove, the excess fluid can be buffered by the detection groove. The flow channel flows to the buffer tank, so that the fluid can be filled in the detection tank to form a predetermined amount. The user does not need to precisely control the amount of fluid injected into each injection tank to accurately make the detection The amount of fluid in the tank is quantified, which can achieve the effect of accelerating work efficiency and improving ease of use.

Wherein, the plurality of first micro flow channels are located on the second surface of the first wafer body, and two ends of each of the first micro flow channels are respectively connected to any two injection grooves. In this way, the first microfluidic channel can be located at the bottom end of the injection groove, which can ensure that all the fluid flows to the first microfluidic channel.

Wherein, the first micro flow channel is connected to two injection grooves in a curved shape. In this way, the time for the fluid to pass through the first microchannel can be prolonged, and the flow rate of the fluid can be slowed down, which has the effect of improving the uniformity of fluid mixing.

Wherein, the second micro flow channel is connected to the collecting groove and the detecting groove in a curved shape. In this way, the time for the fluid to pass through the second microchannel can be prolonged, and the flow rate of the fluid can be slowed down, which has the effect of improving the uniformity of fluid mixing.

Wherein, the collecting groove and the detecting groove penetrate through the first surface and the second surface of the second wafer body, and the second surface of the second wafer body is combined with a closing member. In this way, the closing member is used to block the opening of the collecting groove and the detecting groove on the second surface, and has the function of enabling fluid to be contained in the collecting groove and the detecting groove.

Wherein, the second microfluidic channel is located in the sealing member. In this way, the second microfluidic channel can be located at the bottom end of the collecting tank, which has the effect of ensuring that all fluids can flow to the second microfluidic channel.

Wherein, the second micro flow channel is located on the second surface of the second wafer body. In this way, the second microfluidic channel can be located at the bottom end of the collecting tank, which has the effect of ensuring that all fluids can flow to the second microfluidic channel.

Wherein, the buffer flow channel is located on the second surface of the first wafer body. In this way, the buffer flow channel can be located at the top of the detection groove, which has the effect of ensuring that the fluid can fill the detection groove.

Wherein, the buffer flow channel is located on the first surface of the second wafer body. In this way, the buffer flow channel can be located at the top of the detection groove, which has the effect of ensuring that the fluid can fill the detection groove.

Wherein, the second surface of the first wafer body and the first surface of the second wafer body each have the buffer flow channel. In this way, the buffer flow channel can be located at the top of the detection groove, which has the effect of ensuring that the fluid can fill the detection groove.

Wherein, a cover body is further included, and the cover body is combined with the first surface of the first chip body. The cover body has a plurality of liquid injection holes, and each of the liquid injection holes is located in the injection groove respectively. In this way, the overflow of the fluid in the plurality of injection tanks and the plurality of buffer tanks can be avoided, and the fluid can be injected into the injection tank.

Wherein, the opening of the liquid injection hole is smaller than the opening of the injection groove on the first surface. In this way, the cover can cover part of the area of the opening of the first surface, and has the effect of preventing fluid from overflowing from the opening of the first surface.

In order to make the above and other purposes, features and advantages of this creation more obvious and understandable, the following is a detailed description of the preferred embodiments of this creation, together with the accompanying drawings:

Please refer to Fig. 1, which is a preferred embodiment of the creative hybrid chip, which includes a first chip body 1 and a second chip body 2, the first chip body 1 being stacked on the second chip body 2.

The first wafer body 1 may have a plurality of injection grooves 11, and the plurality of injection grooves 11 can be used to separately inject fluids such as reaction solution or sample solution, and the first wafer body 1 can preferably be glass or acrylic And other light-transmitting materials, so that the hybrid wafer can be applied to detection instruments such as spectrometers, which can be understood by those in the art. The first wafer body 1 may have a first surface 1a and a second surface 1b opposite to each other. The plurality of injection grooves 11 penetrate through the first surface 1a and the second surface 1b, so that the flow system can be The first surface 1a is injected into the plurality of injection grooves 11. Preferably, the volumes of the plurality of injection grooves 11 can be the same or have a certain volume ratio with each other, so that the user can conveniently control the injection volume of the fluid. The first wafer body 1 has a plurality of first micro-channels 12, and the first micro-channels 12 are connected to any two injection grooves 11, so that the fluid injected into one of the grooves 11 can pass through the first micro-channels. The channel 12 flows to another injection tank 11, whereby the fluids in each injection tank 11 can be mixed. In this embodiment, the first microfluidic channel 12 is preferably formed on the second surface 1b by laser ablation, so that the first microfluidic channel 12 can communicate with two injection grooves 11. Preferably, the first micro-channel 12 can be connected between the two injection grooves 11 in a curved shape, so that the time for the fluid to pass through the first micro-channel 12 can be prolonged, and the flow rate of the fluid can be reduced. It can improve the uniformity of fluid mixing.

The connection system of the first microchannel 12 and the plurality of injection grooves 11 can be connected in series, so that the fluids respectively injected into the plurality of injection grooves 11 can be mixed in sequence, or can pass through the plurality of first microchannels. 12 One injection tank 11 is connected to several injection tanks 11 respectively, so that the fluids respectively injected into the several injection tanks 11 can be mixed in one injection tank 11 at the same time, and this creation is not limited. For example, the several injection tanks 11 can respectively inject the same or different reaction liquids and sample containing liquids, so that several reaction liquids can be mixed with the sample containing liquids at the same time. The first wafer body 1 may additionally have at least one buffer groove 13 which can penetrate through the first surface 1a and the second surface 1b, so that excess fluid can flow into the buffer groove 13 from the second surface 1b Inside (detailed later).

The second wafer body 2 has a first surface 2a and a second surface 2b. The first surface 2a can be combined with the second surface 1b of the first wafer body 1, whereby the first surface 2a is The plurality of injection grooves 11 of the first wafer body 1 can be blocked. In this way, fluid can be contained in the injection groove 11 and the buffer groove 13, and the fluid can be ensured to circulate through the first micro channel 12. The second wafer body 2 has a collecting groove 21 for collecting the fluid injected into the injection groove 11. In detail, the collecting groove 21 can be opposed to one of the injection grooves 11 in the first wafer body 1, so that the fluid in the plurality of injection grooves 11 can flow into the collecting groove through the first microchannel 12 21, so that the fluids in the several injection tanks 11 can be mixed and concentrated in the collecting tank 21.

The second wafer body 2 has a detection groove 22, and the detection groove 22 can receive the fluid in the collecting tank 21 for detection. For example, it can be performed by illuminating the fluid in the detection groove 22 by a conventional spectrometer. Detection. In detail, the collection tank 21 and the detection tank 22 can be connected by a second micro flow channel 23, so that the fluid in the collection tank 21 can flow into the detection tank 22 through the second micro flow channel 23. In this embodiment, the collecting groove 21 and the detecting groove 22 can penetrate the first surface 2a and the second surface 2b, and the second surface 2b of the second wafer body 2 can be combined with a closing member 24, The closing member 24 is used to block the openings of the collecting groove 21 and the detecting groove 22 on the second surface 2 b, so that fluid can be contained in the collecting groove 21 and the detecting groove 22. For example, the second microfluidic channel 23 may be located on the closing member 24, and two ends of the second microfluidic channel 23 may be located at the openings of the collecting groove 21 and the detecting groove 22 on the second surface 2b, respectively. As a result, the second micro-channel can be located at the bottom end of the collecting tank 21, and when fluid flows into the collecting tank 21, it can be ensured that the fluid can flow into the second micro-channel 23 at the bottom end of the collecting tank 21. The second micro channel 23 can be formed on the sealing member 24 by laser ablation, or the second micro channel 23 can also be formed on the second surface of the second wafer body 2 by laser ablation 2b, this creation is not restricted.

At least one buffer flow channel 25 can be connected to the buffer tank 13 and the detection tank 22, so that excess fluid flowing into the detection tank 22 can be guided to the buffer tank 13 through the buffer flow channel 25, and the buffer flow channel 25 can Formed by laser ablation, the buffer channel 25 may be located on the second wafer body 2, or may be located on the second surface 1b of the first wafer body 1, or on the second surface of the first wafer body 1. 1b and the first surface 2a of the second wafer body 2 both have the buffer flow channel 25. The present invention is not limited. In this embodiment, the buffer flow channel 25 is located on the first surface of the second wafer body 2 2a. In detail, when the second surface 1b of the first wafer body 1 is bonded to the first surface 2a, the buffer flow channel 25 may be partially aligned with the buffer groove 13, whereby the detection groove 22 is filled After the fluid is filled, the fluid can be injected into the buffer tank 13 through the buffer channel 25.

Please refer to Figures 3 and 4, according to the aforementioned structure, when the hybrid wafer is used in this invention, the sample fluid or the reaction fluid can be respectively injected into the plurality of injection grooves 11, and the hybrid wafer can be placed in a test The platform T is provided with a stable pressure by a fluid pressurizing member P to push the fluid in the mixed wafer. For example, the fluid pressurizing member P can simultaneously input air pressure to several injection grooves 11, and the fluid in each injection groove 11 is pushed by the air pressure to flow into the first microchannel 12, thereby forcing each injection The fluid in the tank 11 flows into the collecting tank 21 of the second wafer body 2 through the first microchannel 12 for mixing. As the fluid pressurizing member P continues to input air pressure, the fluid in the collecting tank 21 can pass through the first Two micro flow channels 23 flow into the detection tank 22, and are injected into the buffer tank 13 of the first wafer body 1 through the buffer flow channel 25. In this way, it is possible to use a conventional spectrometer or other absorbance detection instrument to control the detection tank. The position of 22 is illuminated for detection.

Please continue to refer to Figures 1 and 5, the creative hybrid chip may further include a cover 3 which can be combined with the first surface 1a of the first chip body 1, and the cover 3 can To prevent the fluid in the plurality of injection tanks 11 from overflowing, the cover 3 may also have a plurality of injection holes 31, and each of the injection holes 31 is located in the injection tank 11, so that the fluid can pass through the A liquid injection hole 31 is injected into the injection groove 11. Preferably, the opening of the injection hole 31 can be smaller than the opening of the injection groove 11 on the first surface 1a, whereby the cover 3 can cover The partial area of the opening of the first surface 1a has the effect of preventing fluid from overflowing from the opening of the first surface 1a.

To sum up, the hybrid chip of the present creation has the detection groove through the buffer flow channel through the second chip body. When the fluid is filled into the detection groove, the excess fluid can be The buffer flow channel flows to the buffer tank, so that the fluid can be filled in the detection tank to form a predetermined amount. The user can accurately use the fluid without accurately controlling the amount of fluid injected into each injection tank. The amount of fluid in the detection tank is quantified, which can achieve the effect of accelerating work efficiency and improving ease of use.

Although this creation has been disclosed using the above-mentioned preferred embodiment, it is not intended to limit this creation. Anyone who is familiar with this technique does not depart from the spirit and scope of this creation and makes various changes and modifications relative to the above-mentioned embodiment. The technical scope protected by the creation, therefore, the scope of protection of this creation shall be subject to the scope of the attached patent application.

1: The first chip body 1a, 2a: first surface 1b, 2b: second surface 11: Injection slot 12: The first micro channel 13: buffer slot 2: Second chip body 21: Collection slot 22: detection slot 23: The second micro channel 24: closure 25: Buffer runner 3: cover 31: Injection hole T: Testing platform P: Fluid pressurized parts

[Picture 1] An exploded perspective view of an embodiment of this creation. [Picture 2] A top view of an embodiment of this creation. [Picture 3] The detection situation diagram of an embodiment of this creation. [Picture 4] A cross-sectional view along the line A-A in Fig. 2. [Figure 5] A cross-sectional view of the second embodiment of this creation.

1: The first chip body

1a, 2a: first surface

1b, 2b: second surface

11: Injection slot

12: The first micro channel

13: buffer slot

2: Second chip body

21: Collection slot

22: detection slot

23: The second micro channel

24: closure

25: Buffer runner

3: cover

31: Injection hole

Claims (12)

A hybrid wafer containing: A first wafer body has a first surface and a second surface opposite to each other, a plurality of injection grooves respectively penetrate through the first surface and the second surface, the first wafer body has a plurality of first micro flow channels, The first micro flow channel is connected to any two injection grooves, and the second surface has at least one buffer groove; A second wafer body having a first surface and a second surface opposite to each other. The first surface of the second wafer body is bonded to the second surface of the first wafer body, and the second wafer body has a pair of collecting grooves Located in one of the several injection grooves, a second micro flow channel connects the collection groove and the detection groove; and A buffer flow channel connects the detection groove and the buffer groove. The hybrid wafer of claim 1, wherein the plurality of first micro-channels are located on the second surface of the first wafer body, and two ends of each of the first micro-channels are respectively connected to any two injection grooves. The hybrid wafer of claim 1, wherein the first micro-channel is connected to two injection grooves in a curved shape. The hybrid wafer of claim 1, wherein the second micro flow channel is connected to the collecting groove and the detecting groove in a curved shape. The hybrid wafer of claim 1, wherein the collecting groove and the detecting groove penetrate the first surface and the second surface of the second wafer body, and the second surface of the second wafer body is coupled with a closing member. Such as the hybrid wafer of claim 5, wherein the second micro flow channel is located in the sealing member. The hybrid wafer of claim 1, wherein the second micro flow channel is located on the second surface of the second wafer body. The hybrid wafer of claim 1, wherein the buffer flow channel is located on the second surface of the first wafer body. The hybrid wafer of claim 1, wherein the buffer flow channel is located on the first surface of the second wafer body. The hybrid wafer of claim 1, wherein the second surface of the first wafer body and the first surface of the second wafer body each have the buffer flow channel. For example, the hybrid wafer according to any one of claims 1 to 10, further comprising a cover body bonded to the first surface of the first wafer body, the cover body having a plurality of liquid injection holes, each of the liquid injection holes The lines are located in the injection groove respectively. The hybrid wafer of claim 11, wherein the opening of the liquid injection hole is smaller than the opening of the injection groove on the first surface.

Images