TWM572456U - Probiotic screening device - Google Patents

Abstract

A probiotic screening device is used to solve the problem that the conventional probiotic screening method requires a large amount of manpower for detection and analysis. The system comprises: an incubator for co-cultivating a plurality of samples with a plurality of probiotics to form a plurality of samples to be tested; a first detecting module and a second detecting module for detecting each of the waiting bodies The first detection data and the second detection data are coupled to the first detection module and the second detection module to receive the plurality of first data and the processing unit a plurality of second data, the processing unit respectively selecting an optimal value of the two of the plurality of first data and the plurality of second data, and analyzing whether the optimal values of the two are from the same test to be tested Body, if yes, controls an output unit to output a coincidence signal.

Description

Probiotic screening device

This creation is about a probiotic screening device, especially a probiotic screening device that can automatically perform detection and analysis of test results.

Probiotics are a group of microorganisms that help human health, including bacteria (such as lactobacilli, Bifidobacterium, etc.) and fungi (such as yeast, anthrax, etc.). According to research, probiotics can effectively stimulate the human body. Produces immune-related cytokines to regulate the body's immune system. However, the same probiotics are used by different people, and the effects are not the same. More specifically, because each person's genes are different, different people will react differently to the same probiotic. Therefore, the best effect of using probiotics can be achieved by screening the most suitable probiotics for each user.

A conventional method for screening probiotic bacteria is to co-cultivate a plurality of samples taken from a subject with different kinds of probiotics, and detect the amount of cytokine produced by each sample by molecular detection method. In order to screen out the probiotics most suitable for the subject. Similar to the conventional probiotic screening method, it is disclosed in the Republic of China Announcement No. I503545 "Probiotics Screening Method for Individualized Differences" patent case. However, the conventional probiotic screening method still requires a large amount of manpower to perform the detection method and the analysis of the detection result, which may cause trouble for the inspector when performing a large number of screenings.

In order to solve the above problems, the purpose of the present invention is to provide a probiotic screening device which is capable of automatically performing detection and automatically analyzing the detection results.

The following directional or similar terms, such as "before", "after", "left", "right", "upper (top)", "lower (bottom)", "inside", "outside" The "lateral" and the like are mainly referred to the orientation of the additional drawings, and the directionality or its approximation is only used to assist in the description and understanding of the embodiments of the present invention, and is not intended to limit the present invention.

The probiotics described in the entire text of this creation refer to bacteria or fungi that are helpful to human health. The cytokine may be any cytokine associated with the immune system such as interleukin, interferon, tumor necrosis factor or transforming growth factor.

The test system is peripheral blood cells or oral immune cells taken from the same subject.

The probiotic screening device of the present invention comprises: an incubator having a plurality of co-cultivation spaces, wherein the co-cultivation space is used for co-cultivating a sample with a plurality of probiotics respectively to form a a test module, the incubator has a sensing module for sensing an environmental factor in the incubator, and the incubator is connected to a ring control module to adjust an environmental factor in the incubator to a fixed value; a first detecting module for detecting each of the objects to be tested to generate a first data; a second detecting module for detecting each of the objects to be tested to generate a second data; And a processing unit, coupled to the sensing module, the ring control module, the first detecting module and the second detecting module, wherein the processing unit is configured to select an optimal one of the plurality of first data And selecting an optimal value from the plurality of second data, the processing unit analyzing whether an optimal value of the plurality of first data and an optimal value of the plurality of second data are from the same Test the sample, if the analysis result is yes, control an output unit to output a phase Signal.

Accordingly, the probiotic screening device of the present invention is capable of automatically detecting each of the samples to be tested and interpreting the detection result by the first detection module and the second detection module, and automatically analyzing the detection unit by the processing unit. The result is detected, and the screening result is notified to the inspector by the output unit. Thereby, the testing personnel can obtain the analysis result without detecting the test object and analyzing the test result in person, thereby achieving the effect of saving manpower and improving the convenience of screening of the probiotic bacteria.

The processing unit analyzes whether the optimal value of the first data and the optimal value of the second data are from the same test object, and if the analysis result is no, the output unit is controlled to output a discrepancy signal. In this way, it has the effect of notifying the inspector that the screening has to be performed again.

The sensing module can be a temperature sensor, a relative humidity sensor or a carbon dioxide concentration sensor. Thus, there is an effect of sensing the temperature, humidity or carbon dioxide concentration in the incubator.

The ring control module can be a temperature controller, a relative humidity controller or a carbon dioxide concentration controller. Thus, there is an effect of regulating the temperature, humidity or carbon dioxide concentration in the incubator.

Wherein, the incubator preferably has a plurality of probiotic bacteria troughs and a probiotic adder, wherein the probiotic bacteria trough and the probiotic adder are located in the incubator, and the probiotic adder connects the plurality of probiotic bacteria troughs The processing unit is coupled to the probiotic adder to control the probiotic adder to take the plurality of probiotics from the plurality of probiotic troughs and add the plurality of co-culture spaces to the sample. In this way, probiotics can be automatically added to the plurality of samples to achieve a labor saving effect.

Wherein, the probiotic adder has a plurality of extraction tubes, a moving part and a plurality of dripping parts, each of the connecting tubes connecting different probiotic bacteria troughs, the moving piece connecting the plurality of dripping parts, each of the dripping parts Connect the different extraction tubes. Thus, there is an effect that the plurality of probiotics can be accurately added to the plurality of samples.

The optimal value among the plurality of first data is the highest value among the plurality of first data, and the optimal value among the plurality of second data is the highest value among the plurality of second data. In this way, it has the effect of quickly performing optimal value screening.

Wherein, the first detecting module detects each of the objects to be tested by an enzyme-linked immunodot method, and the first detecting module counts the number of spots generated by each of the objects to be tested, and each of the samples to be tested is to be tested. The number of spots measured by the sample is input to the first data, and the second detecting module detects each of the samples to be tested by immunocytochemical staining, and the second detecting module generates each of the samples to be tested. Fluorescence is performed on the relative fluorescence unit, and the relative fluorescence unit value measured by each of the samples to be tested is input to the second data. In this way, each of the samples to be tested can be detected by two different methods, so that the first data and the second data can be compared with each other, which has the effect of improving the screening accuracy.

In order to make the above and other objects, features and advantages of the present invention more comprehensible, the preferred embodiments of the present invention are described in detail below with reference to the accompanying drawings.

Referring to FIG. 1 , an embodiment of the present probiotic screening device includes an incubator 1 , a first detecting module 2 , a second detecting module 3 , a processing unit 4 , and a The output unit 5 is coupled to the incubator 1, the first detecting module 2, the second detecting module 3, and the output unit 5.

In detail, please refer to FIG. 2 , the incubator 1 has a sensing module 11 , and the sensing module 11 is located in the incubator 1 for sensing an environmental factor in the incubator 1 . And generate an environmental signal. The culture box 1 is connected to a ring control module 12 for receiving the environmental signal and adjusting the environmental factor in the incubator 1 to a predetermined value according to the environmental signal. Specifically, the environmental factor may be temperature, relative humidity or carbon dioxide concentration, and the sensing module 11 may be a temperature sensor, a relative humidity sensor or a carbon dioxide concentration sensor, and the ring mode Group 12 can be correspondingly a temperature controller, a relative humidity controller or a carbon dioxide concentration controller. For example, the sensing module 11 is in the condition of the temperature sensor, and the temperature sensor can sense the temperature in the incubator 1 as the environmental signal, and the environmental signal can be controlled by the temperature. The device (the environmental control module 12) accepts and adjusts the temperature in the incubator 1 until the temperature in the incubator 1 reaches the predetermined value according to the difference between the environmental signal and the predetermined value.

The culture box 1 also has a plurality of co-cultivation spaces 13 respectively, and each of the co-culture spaces 13 is used to provide a sample S. For example, the culture box 1 can accommodate a porous disk P. Each of the holes in the porous disk P serves as one of the co-culture spaces 13, so that the plurality of samples S can be conveniently placed in each of the co-culture spaces 13, but not limited thereto. The sample S accommodated in each of the co-culture spaces 13 can be co-cultured in the co-culture space 13 after being mixed with a probiotic, thereby generating a sample to be tested, respectively. As shown in FIG. 2, in the present embodiment, the two specimens S can form a first sample to be tested A and a second sample to be tested B after being co-cultured with the probiotics.

The incubator 1 may have a plurality of probiotic tanks 14 and a probiotic adder 15 each of which may be stored by a probiotic. The probiotic adder 15 is for taking out the probiotic bacteria in the plurality of probiotic cells 14 and adding them to the sample S of each of the co-culture spaces 13. In the present embodiment, the probiotic adder 15 has a plurality of extraction tubes 151, a moving member 152 and a plurality of drip members 153, each of which is connected to a different probiotic trough 14 so that each of the extracting tubes 151 only one probiotic was taken. The moving member 152 may be a group of slide rails having two vertical axes, and the moving member 152 connects the respective drip members 153 so that each of the drip members 153 can be moved above the plurality of specimens S. Each of the drip members 153 is connected to the different extraction tubes 151 to add the probiotics to which the connected extraction tubes 151 are taken out to the plurality of specimens S. Thus, the probiotic adder 15 can accurately add the plurality of probiotics to the plurality of samples S.

Referring to FIG. 1 , the first detecting module 2 is configured to detect the amount of cells capable of secreting cytokines in each sample to be tested to generate a first data. In this embodiment, the first detecting module 2 detects each of the samples to be tested by an enzyme-linked immunoassay (ELISPOT assay), but is not limited thereto. The enzyme-linked immunoassay method causes cells that secrete cytokines to develop spots by a color reaction. In other words, the number of spots represents the amount of cells that can secrete cytokines. After the spot is generated, the first detecting module 2 counts the spots of each of the samples to be tested, and forms the first data according to the data format of the sample to be tested, the number of spots, for example, the first The sample A to be tested produces 10 spots; the second sample B to be tested produces 23 spots, that is, two first data of [A, 10] and [B, 23] are stored.

The second detecting module 3 is configured to detect the amount of cytokines in each of the samples to be tested to generate a second data. However, the detection method used by the second detection module 3 is different from the detection method used by the first detection module 2. In this embodiment, the second detection module 3 performs immunocytochemical staining on each of the samples to be tested by fluorescently labeled antibodies, but is not limited thereto. The immunocytochemical staining method uses a specific reaction between an antibody and an antigen to stain the cytokines in the cells, and measures the relative fluorescence units (RFU) of each sample to be tested by fluorescence staining. That is, the relative expression amount of the cytokines in each of the samples to be tested can be estimated. After the dyeing is completed, the second detecting module 3 measures the relative fluorescent units generated by each of the samples to be tested, and forms the plurality of data formats according to the data format of the sample to be tested and the relative fluorescent unit value. The second data, for example, the measured value of the first sample to be tested A is 420 RFU; the measured value of the second sample to be tested B is 970 RFU, that is, [A, 420] and [B, 970] The second data is stored.

The processing unit 4 can be any circuit unit having functions of data processing, signal generation, and signal control, and can be, for example, a microprocessor, a microcontroller, a digital signal processor, or a logic circuit. In this embodiment, The processing unit 4 can be a microprocessor, but is not limited thereto.

The processing unit 4 can be coupled to the sensing module 11 of the incubator 1 and the ring control module 12, whereby the environmental signal output by the sensing module 11 can be communicated to the environmental control unit via the processing unit 4. The module 12 enables the environmental control module 12 to adjust the environmental factor in the incubator 1.

Moreover, the processing unit 4 can be coupled to the probiotic adder 15 of the incubator 1 to enable the processing unit 4 to control the probiotic adder 15, whereby the probiotic adder 15 can automatically number the probiotics Probiotics are added to the plurality of samples S. In this way, the manpower can be further reduced and the convenience of use can be improved.

The processing unit 4 can be coupled to the first detection module 2 and the second detection module 3, so that the processing unit 4 can receive the plurality of the first detection module 2 and the second detection module 3 The first data and the plurality of second data. In this embodiment, the processing unit 4 may select the first data having the most number of spots as the optimal value from the plurality of first data, and select the highest relative fluorescent unit value from the plurality of second data. The second data is taken as the best value. For example, the plurality of first data are [A, 10] and [B, 23], and the optimal value of the first data is [B, 23]; the second data is [A, 420] ] and [B, 970], the optimal value of the second data is [B, 970]. Then, the processing unit 4 can analyze the plurality of first data by comparing whether the sample code of the object to be tested and the optimal value of the second data of the first data are the same. And whether the optimal value of the plurality of second data is from the same sample to be tested.

The output unit 5 is used to notify the tester of the result of the analysis. Since the output unit 5 is coupled to the processing unit 4, the processing unit 4 can control the output unit 5. If the processing unit 4 analyzes that the optimal values of the plurality of first data and the plurality of second data are from the same test object, the processing unit 4 controls the output unit 5 to output a coincidence signal. The coincidence signal may include a matching code and a screening result, and the matching code represents the two detection results, and the screening result is the code of the selected sample to be tested. The tester can use the screening result to find the probiotic that is most suitable for the subject. If the processing unit 4 analyzes that the optimal values of the plurality of first data and the plurality of second data are not from the same subject to be tested, the processing unit 4 controls the output unit 5 to output a discrepancy signal. The discrepancy signal contains a non-conformity code, which means that the two test results do not match. The tester can learn that the two test results do not match by the discrepancy code, and can be re-screened.

In summary, the probiotic screening device of the present invention is capable of automatically detecting each of the samples to be tested and interpreting the detection result by the first detecting module and the second detecting module, and using the processing unit The detection result is automatically analyzed, and the screening result is notified to the inspector by the output unit. Thereby, the testing personnel can obtain the analysis result without detecting the test object and analyzing the test result in person, thereby achieving the effect of saving manpower and improving the convenience of screening of the probiotic bacteria.

Although the present invention has been disclosed by the above-described preferred embodiments, it is not intended to limit the present invention, and it is still within the spirit and scope of the present invention to make various changes and modifications to the above embodiments. The technical scope of the protection is created, so the scope of protection of this creation is subject to the definition of the patent application scope attached.

1‧‧‧ incubator

11‧‧‧Sensor module

12‧‧‧Environmental Control Module

13‧‧‧Cultivating space

14‧‧‧Probiotics trough

15‧‧‧Probiotic Adder

151‧‧‧ extraction tube

152‧‧‧Mobile parts

153‧‧‧ dripping parts

2‧‧‧First detection module

3‧‧‧Second test module

4‧‧‧Processing unit

5‧‧‧Output unit

A‧‧‧First test object

B‧‧‧Second test object

P‧‧‧ porous disk

S‧‧‧ specimen

[Fig. 1] A system architecture diagram of an embodiment of the present probiotic screening device. [Fig. 2] A perspective structural view of an incubator of an embodiment of the present probiotic screening device.

Claims (8)

A probiotic screening device comprises: an incubator having a plurality of co-cultivation spaces, wherein each co-cultivation space is used for co-cultivation of a sample with a plurality of probiotics respectively to form a test to be tested a sample body having a sensing module for sensing an environmental factor in the incubator, the incubator being connected to a ring control module to adjust an environmental factor in the incubator to a fixed value; a detection module for detecting each of the samples to be tested to generate a first data; a second detection module for detecting each of the samples to be tested to generate a second data; The unit is coupled to the sensing module, the ring control module, the first detecting module, and the second detecting module, wherein the processing unit is configured to select an optimal value from the plurality of first data, and Selecting an optimal value from the plurality of second data, the processing unit analyzing whether an optimal value of the plurality of first data and an optimal value of the plurality of second data are from the same sample to be tested And an output unit coupled to the processing unit, if The processing unit analyzes the optimum value of the number of the first data and the optimum value of the plurality of second data from the same test specimen, the processing unit controls the output unit outputs a match signal. The probiotic screening device according to claim 1, wherein the processing unit analyzes whether the optimal value of the first data and the optimal value of the second data are from the same sample to be tested, if the analysis If the result is no, the output unit is controlled to output a discrepancy signal. The probiotic screening device of claim 1, wherein the sensing module is a temperature sensor, a relative humidity sensor or a carbon dioxide concentration sensor. The probiotic screening device of claim 1, wherein the environmental control module is a temperature controller, a relative humidity controller or a carbon dioxide concentration controller. The probiotic screening device according to the first aspect of the invention, wherein the incubator has a plurality of probiotic bacteria tanks and a probiotic adder, wherein the plurality of probiotic bacteria tanks and the probiotic adder are located in the incubator The probiotic adder is connected to the plurality of probiotic bacteria tanks, and the processing unit is coupled to the probiotic adder to control the probiotic adder to take out the plurality of probiotic bacteria from the plurality of probiotic bacteria tanks and add the probiotics Several specimens in the co-culture space. The probiotics screening device of claim 5, wherein the probiotic adder has a plurality of extraction tubes, a moving member and a plurality of drip members, each of the extraction tubes connecting the different probiotic bacteria tanks, The moving member connects the plurality of drip members, and each of the drip members connects different ones of the extracting tubes. The probiotic screening device according to claim 1, wherein the optimal value among the plurality of first data is the highest value among the plurality of first data, and the most of the plurality of second data The good value is the highest value among the plurality of second data. The probiotic screening device according to claim 7, wherein the first detecting module detects each of the samples to be tested by an enzyme-linked immunological point method, and the first detecting module is to be tested. The number of spots generated by the sample is counted, and the number of spots measured by each sample to be tested is input into the first data, and the second detection module detects each sample to be tested by immunocytochemical staining. The second detecting module performs a relative fluorescence unit measurement on the fluorescence generated by each of the objects to be tested, and inputs the relative fluorescent unit value measured by each of the objects to be tested into the second data.