TWM562971U - System for using drosophila unit to detect food safety and bio-health activity of food - Google Patents

Abstract

The present invention discloses a detection system for detecting food safety and biological activity of foods and beverages using Drosophila melanogaster as an animal model. Test items include exercise capacity, fertility, hunger tolerance, antioxidant capacity (including anti-hydrogen peroxide and anti-paraquat oxidative stress), body weight test and life observation experiment to detect various foods and beverages. The biological effects of flies. The system is a medium for mixing the food or beverage to be tested into the Drosophila unit according to the ratio of eating or drinking in a healthy group. After feeding the Drosophila unit for a predetermined period of time, the Drosophila unit feeding the control medium is used as a control to detect the above. Biological activity to define whether the food or beverage to be tested has a negative impact on the health of the fruit fly or has a health enhancing effect.

Description

System for detecting food safety and bio-health activity of food using Drosophila unit

This creation belongs to the field of food health biotechnology, and more specifically, to providing a system for detecting food safety using a fruit fly unit or for identifying whether a food or drink has enhanced or reduced bio-health activities.

Food safety is an interdisciplinary field that focuses on ensuring food hygiene and food safety, reducing disease hazards, and preventing food poisoning during food processing, storage, and sales. Through the risk analysis of science, the risk of harm to consumers' life and health, and then the development of control measures to ensure food safety, food safety measures to eliminate or reduce the risk to consumers' lives and health, is the core of food safety.

Nowadays, due to the flow of various chemical substances in the environment, it directly pollutes the soil, air and water, indirectly affects the fruits and vegetables, and the arbitrarily added food, such as pesticides, hormone interferences, personal care medical supplies and high-tech industrial pollutants. Classified as an emerging pollutant. It can be seen that pollutants are ubiquitous and organisms are exposed to single or mixed pollutants, posing a potential health hazard. How to prevent toxic substances from continuously expanding the scope of pollution, affecting the health of the organism and the continuation of life, it is extremely important to check whether the diet is safe.

In recent years, a series of major food safety incidents have emerged in Taiwan, such as the plasticizer incident in 2011; the poisonous starch incident in 2013 and the black liquor (the import of cottonseed oil from China); the 2014 waste oil incident. In addition to pollution and poisoning problems, there are also problems of deliberate illegal use of additives, misrepresentation and counterfeiting; the former is caused by poor quality of food manufacturing environment and technology, while the latter is professional knowledge and Misuse and abuse of technology. Food safety incidents seriously endanger the health and even life of Chinese people, affect Taiwan's economic development, combat the confidence of the Chinese people, and undermine the image of Taiwan and the government.

Current food testing programs include: analysis of food nutrients (such as various nutrient content ratios, calories, sugar, salt, oil and fats, etc.); residue analysis (such as pesticide residues, growth hormone residues, microbial toxins, antibiotic residues, etc.); Microbiological analysis and analysis; whether the content of various food additives such as preservatives, sweeteners, leavening agents, fragrances, food colors, etc. is within the specified safe range; other special substances and pollutants (such as sulfur dioxide (SO ₂ ), melamine) (Melamine), formaldehyde (formaldehyde), coumarin (Coumarin), hydrogen peroxide, etc.). Analysis of food characteristics and specific ingredients (such as heavy metal content, plasticizer, milk fat, Nitrogen of Amino acid, Nitrogen of Formaldehyde, Nitrite, water activity ( Water Activity), Brix, Acidity, Melting Point, pH Value, Salty Value, Specific Gravity, Total Solid, Volatile Volatile Basic Nitrogen, Foreign Matters, Food Additives-water-insoluble Calcium Propionate, Freshness Meter, Cholesterol ), Caffeine, Methanol in Wine, Soybean GMO Test, Corn GMO Test, etc.

These testing items are of course vital. However, the current testing systems are aimed at assessing the known contamination and toxicity of a single substance, but there is no guarantee that the safety of the human body or the living body will not be achieved if these safety standards are met. There will be an impact, especially the cumulative effect of long-term consumption cannot be determined. On the other hand, there are many types of food additives, and there are often a variety of additives in a food. These additives alone may not have a significant negative impact on health if they are safe, but when a food contains multiple additives. At the time of the object, even if it is in the safe range, its overall cumulative total toxicity has caused great damage to the organism, especially when it is consumed for a long time. In addition, there are some unidentified substances outside these testing items. Many toxic substances are emerging pollutants and difficult to judge. In addition, genetically modified foods, especially beans, account for a large proportion of the food market, and its safety, especially long-term cumulative effects, is unknown. For these problems, there is currently no effective detection method. The most straightforward method is to use animal models to measure and use these foods to feed animals directly to test their effects on the health of the living body. The fast-screening animal model provides multi-faceted biotoxicity assessment indicators, and establishes an organism to test the overall toxicity of harmful substances to achieve dietary safety and health control, ensuring food safety and health for Chinese people. However, to date, there have been no precedents for using animal models to detect food safety.

Animal models are the most important research objects in the field of life science research, and have played an important role in promoting the progress of human biomedicine. Most of the biomedical Nobel Prizes in recent years have been the result of animal experiments. Current animal models have gradually shifted from large vertebrates to small animals, because large animals require more energy for cultivation and have a long life cycle, which is not suitable for large population studies. Some small animals, such as flies, nematodes, zebrafish, etc., because of their short life cycle, are easy to breed and breed, have low cost, and are extremely rich in genetics, and gradually become equivalent in modern biomedical research with animals such as mice. Important animal models, many of the above experiments in mice, can be performed in these animals, and the required experimental data can be obtained in a faster, more economical manner. Considerable achievements in various aspects, such as the mechanism of aging and longevity, especially in drug screening, have a strong advantage. If mice are used for drug screening, it is very expensive and takes longer.

Drosophila has an earlier history as an animal model than nematodes and zebrafish, and its research is more thorough. Drosophila, as a model organism, is relatively simple and inexpensive to operate, has a short life history and is easy to breed. Under standard culture conditions, the production time at 25 ° C is from egg to adult 12-14 days. The life cycle of Drosophila has four distinct stages: embryos, larvae, pupa and adults. Various tests are available at each stage. Larvae and adult fruit flies are easy to administer by directly adding foods, medicines, etc. to foods, and can mimic human feeding routes without injection. In fruit flies, various test methods have been designed and developed. The most common assays examine changes in the body structure of adults, such as the eyes and wings. Common adult fruit fly behavioral analyses assess simple motor behavior including overall activity, exercise capacity (upward climbing), phototaxis or chemistry toward chemicals.

Drosophila has considerable similarities with higher animals and humans in terms of life structure and vital characteristics. Important genes found in Drosophila research, homologous genes (ie, genes with similar sequences and equal functions) can be found in humans or other higher organisms. The oncogenes found in Drosophila cells also have similar genes in humans. Not only that, but the regulation pathways of gene expression in Drosophila and human cells are also very similar. Drosophila research in cell biology, developmental biology and molecular biology shows that Drosophila has many commonalities with other organisms including humans, and its research results are very helpful in understanding the mechanism of cancer formation, human congenital Major problems such as deformity and the development of human embryos. In addition, like humans, fruit flies have a variety of high-level complex consciousness and behavior, such as courtship, circadian rhythm and even associative learning and memory, and have similar molecular and cellular mechanisms of learning and memory. In addition, due to its short life cycle (survival for about two months), it is easy to study the mechanisms related to anti-aging and longevity.

The research themes of Drosophila is very broad, including embryonic development, nerve conduction, biological clock, learning and memory, and stem cells. In recent years, human disease has become another major direction of fruit fly research due to the formation of the concept of translational medicine. A laboratory study by Dr. Bier of the University of California San Diego in the United States followed by a systematic analysis. They analyzed 714 human disease genes associated with 929 human diseases and found that 548 Drosophila gene sequences are similar. These diseases include developmental defects such as hearing, vision, immunity, nerves, metabolism, and various types of cancer. The unique advantage of Drosophila research is the ability to identify a group of genetic and cellular pathways that work together to facilitate speculation and search for genes and their functions and mechanisms. At present, Drosophila has successfully developed animal models of various diseases, such as various neurological diseases (such as Alzheimer's disease, Parkinson's disease, Huntington's disease, neurodegenerative diseases, etc.), cardiac dysfunction, and metabolic properties. Diseases include diabetes, as well as immune diseases, cancer, kidney stones, etc., and considerable progress has been made in the study of disease mechanisms. Recently developed Drosophila model metabolic disease: Drosophila larvae over-fed in sucrose, consistent with signs of type 2 diabetes. In addition, adult insulin flies show signs of type 1 diabetes when insulin-producing cells (IPC) are inactivated by genetic methods (Musselman et al., 2011). Interestingly, symptoms of diabetes-like symptoms were alleviated when these IPC-deficient Drosophila were injected with insulin (Haselton and Fridell, 2010).

A large number of studies have shown that dietary factors can affect the fertility, health and longevity of fruit flies. High-fat diets have been shown to cause cardiac dysfunction in Drosophila. A diet that is too low or too hot will shorten the lifespan of fruit flies (Birse et al., 2010). A moderately low-calorie diet can significantly extend the lifespan of fruit flies, a condition known as calorie restriction (Bauer et al., 2007). Interestingly, different types of meals have different effects. For example, carbohydrates and proteins in the diet have opposite effects on the physiology of fruit flies: high carbohydrates can lead to weight gain and fat accumulation, while high protein diets can reduce fruit fly weight and increase the reproductive capacity of fruit flies. (Skorupa et al., 2008).

In view of the above-mentioned problems, the object of the present invention is to provide a system for detecting food safety or bio-health activity using a fruit fly unit, comprising: at least two breeding tanks each having at least one side wall and a bottom, wherein the side walls are surrounded and combined The bottom, the side wall and the bottom define an accommodating space, and the accommodating space is respectively placed in the control medium and the food medium containing the food to be tested; the fruit flies are contained, and respectively placed in the accommodating space containing the control medium and containing the food to be tested a medium containing a space for feeding at least two Drosophila units for a predetermined time; and a physiological detecting component disposed outside the at least two breeding tanks, wherein the Drosophila unit feeding the control medium and the food to be tested are fed The Drosophila unit of the culture medium is transferred to the physiological test component for the test item, and the test data of the Drosophila unit fed with the control medium and the food medium to be tested is compared to obtain the result. The result is used to assess the food safety or bio-health activity of the food to be tested.

Preferably, the detection item may include at least one of athletic ability, reproductive ability, hunger tolerance test, oxidative stress test, life observation and memory analysis when detecting the food safety of the food to be tested.

Preferably, when detecting the bio-health activity of the food to be tested, the detection item may include at least one of exercise ability, fertility ability, starvation tolerance test, oxidative stress test, life observation, body weight test and memory analysis. .

Preferably, when the system is detecting exercise capacity, the physiological detection component can be a culture tube having a scale to determine the distance that the fruit fly unit can climb or fly in the culture tube.

Preferably, when the system is detecting fertility, the physiological detection component can be a counting device for counting the number of ovulations of the fruit fly unit. Furthermore, the detection of fertility can be performed by counting devices to count the number of ovulations of the offspring of the Drosophila unit, and the male and female Drosophila can also be fed separately for a predetermined period of time before the detection of reproductive ability.

Preferably, when the system is performing a starvation tolerance test, the physiological detection component can be a culture tube containing agar and water, and the number of deaths of the fruit fly unit is recorded every predetermined time.

Preferably, when the system is subjected to an oxidative stress test, the physiological test component may be a culture tube containing a mixture of hydrogen peroxide or balazone and a glucose solution, and the number of deaths of the fruit fly unit is recorded every predetermined time.

Preferably, when the system performs the memory analysis, the physiological detecting component is a memory testing device, and the memory testing device comprises: a training tube of a hollow tube body, the training tube has a vent hole at one end and is disposed on the The electric shock portion of the hollow tube body is configured to apply at least one of the odor and the electric shock to the training tube; and the test tube of the hollow tube body, the two ends of the test tube respectively have a vent hole for applying the odor to the test tube . Wherein, the Drosophila unit is first placed in the training tube, and the Drosophila unit is subjected to an electric shock and a first odor, and a second odor is administered after a predetermined time without an electric shock, and then the trained Drosophila unit is It is placed in the middle of the test tube, and the first odor is applied to one end of the test tube and the second odor is applied at the other end to determine the memory ability by the Drosophila unit in the moving direction of the test tube.

Preferably, when the system is performing a body weight test, the physiological detecting component may be a weighing device, and the weight of the fruit fly unit is weighed by the weighing device.

Preferably, the fruit fly in the system can be a wild type fruit fly or a fruit fly used in a laboratory. In addition, the fruit fly may be an adult of a fruit fly or a larva of a fruit fly.

Preferably, the food to be tested used in the system may be a food that can be eaten or consumed or a beverage that can be eaten or consumed.

Preferably, the food to be tested in use of the system may be an organic food.

Preferably, the food medium to be tested in the system contains one or more foods to be tested.

According to the present invention, a system for detecting food safety or biohealth activity using a fruit fly unit can have the following advantages:

(1) According to the system of the present invention, fruit flies can be used to measure the food safety or bio-health activity of one or more foods to be tested in a short period of time, including the learning ability, the function of the advanced nervous system of memory, and the reproductive ability.

(2) According to the system for detecting biohealth activity of the present invention, a foodstuff to be tested, such as an organic food, can be fed to a fruit fly, and various biological tests can be performed to evaluate whether the food to be tested has an effect of enhancing bio-health activity.

(3) According to the system for detecting food safety according to the present invention, the food to be tested, such as genetically modified food, can be fed to the fruit fly, and various biological tests can be performed to evaluate the food safety of the food to be tested.

(4) According to the system for detecting food safety according to the present invention, the food to be tested, for example, containing various additives (for example, food coloring, leavening agent, preservative, etc.) can be fed to the fruit fly, and various biological tests can be performed to evaluate Food safety of the food to be tested.

The embodiments of the system for detecting food safety and the bio-health activity of the food according to the present invention will be described below with reference to the related drawings. For ease of understanding, the same elements in the following embodiments are denoted by the same reference numerals.

Please refer to Figure 1, which is a schematic diagram of a system for detecting food safety or biohealth activity. As shown, the present system 100 for detecting food safety or biohealth activity comprises a breeding tank 110, a fruit fly unit 120, and a physiological detection assembly 130. The breeding tanks 110 respectively have at least one side wall and a bottom, and the side walls surround and combine with the bottom, and the side walls and the bottom define an accommodating space for accommodating the medium. The number of the breeding tanks 110 of the present embodiment is two, and the number thereof is not limited thereto, and may be three or more. That is, the number of the breeding tanks 110 may correspond to a total of the number of foods to be tested plus the amount of the control medium 111, but at least two. In the present embodiment, one of the accommodation spaces in the breeding tank 110 accommodates the control medium 111, and the other accommodation space accommodates the food medium 112 to be tested containing the food to be tested. In another embodiment, if the number of foods to be tested to be detected is two or more, the accommodation space of one of them accommodates the control medium 111, and the breeding tanks 110 corresponding to the quantity of the food to be tested are respectively accommodated. The food medium 112 to be tested containing different foods to be tested.

In addition, the control medium 111 is prepared by using the internationally accepted standard culture chicken, including the following components: glucose (D-Glucose), white sugar, seaweed powder, corn flour and yeast powder mixed with water in a certain ratio, After cooling to 55 to 65 ° C, an appropriate amount of preservative (such as propionic acid, benzyl formate) is added, and then dispensed into the Drosophila tube for use, but is not limited thereto. That is, the formulation of the control medium 111 can be changed according to the needs of the test item, for example, when performing the body weight test, the control medium 111 containing 10% to 20% of high fat can be used. The food medium 112 to be tested is prepared by using the food or beverage to be tested as a food to be tested, and adding it in an amount of 10 to 40% (% by weight or volume%) (refer to the respective examples in the ratio of use) in the medium production process. In standard medium. Furthermore, the food medium 112 to be tested may contain one or more foods to be tested. For example, a food medium 112 to be tested may contain only one food to be tested, and another food medium 112 to be tested may contain two or more kinds of foods to be tested, and then it can be observed whether two kinds of foods to be tested are produced simultaneously. Interactions cause physiological changes to the Drosophila unit 120.

In addition, the food to be tested used in the creation may include organic foods, genetically modified foods, and foods containing various food additives. The organic food may be, for example, organic miscellaneous grains (soya beans, mung beans, etc.), organic fruits, organic vegetables, organic tea leaves, organic livestock and poultry products, organic aquatic products, organic milk powder, and processed products thereof, but are not limited thereto. . The genetically modified food may be, for example, a genetically modified cereal (corn, soybean, etc.), a genetically modified fruit (papaya, tomato, etc.), a genetically modified vegetable, or a processed product thereof, but is not limited thereto. The food additive can be, for example, a bactericide (hydrogen peroxide), a food coloring (food yellow No. 5, edible red No. 7 aluminum lacquer, edible blue No. 1, etc.), a leavening agent (sodium alum, potassium alum, etc.), antiseptic Agents (alienic acid, calcium propionate, etc.), sweeteners (D-sorbitol, glycyrrhizin, etc.), bleaches (potassium sulfite, sodium bisulfite, etc.) and antioxidants (dibutylhydroxytoluene, L-ascorbic acid, etc., etc., but is not limited thereto.

The Drosophila unit 120 line comprises a fruit fly, and is respectively placed in an accommodation space containing the control medium 111 and an accommodation space containing the food medium 112 to be tested to feed the fruit fly. In this example, the wild type flies of the Canton S (CS) line were collected within 3 days, and the male and female were separated. (In addition to the test of reproductive ability and learning and memory ability, the test items of the present invention generally use the male fruit fly experiment. ). Each 30 to 35 fruit flies are used as a Drosophila unit 120, and each Drosophila unit 120 is placed in a control medium 111 or a food medium 112 to be tested, respectively, for raising for a predetermined period of time. Drosophila melanogaster (fruit fly) has a short generation period, with an average of two generations, and a pair of fruit flies can produce hundreds of offspring. In detail, male fruit flies can reach sexual maturity 12 hours after emergence, and female fruit flies can reach sexual maturity 8 hours after emergence. Female fruit flies can lay 2 to 20 eggs per hour at 25 ° C, and the sperm obtained from female mating at one time can supply about 6 to 8 times the amount of sperm needed for insemination (ie, without re-mating) spawning). Male fruit flies can be mated to 10 female fruit flies daily. Therefore, compared to other animal models, such as rats and mice, Drosophila can significantly shorten the time of the experiment and allow large-scale experiments. Therefore, it can be applied to detect the impact of a large number of foods or beverages on the vitality and bio-health activity of fruit flies. In addition, fruit flies are not controversial in animal ethics and are consistent with the principle of not harming abuse. Therefore, fruit flies are an excellent model for detecting the vitality and bio-health activity of food safety and food and beverage. In the present embodiment, in addition to the detection of fertility, the male detection adult male fly is used as the fruit fly unit 120. However, the present creation is not limited thereto, and the larva of Drosophila can also be used as the fruit fly unit 120.

The physiological detection component 130 is disposed outside the breeding tank 110, and the feeding control medium 111 or the Drosophila unit 120 feeding the food medium 112 to be tested for a predetermined time is moved to the physiological detecting component 130 to perform a detection item, and the feeding control medium 111 is compared. The test data of the Drosophila unit 120 with the food medium 112 to be tested is used to obtain a result, and the food safety or bio-health activity of the food to be tested is evaluated by the result.

In the present embodiment, the physiological testing component 130 is designed based on the health food management law and the dosage conditions of the literature, and the various foods and beverages to be tested for the fruit fly are tested with reference to the Nutritive Values of Foods. The impact of vitality or bio-health activity, and then the food safety or bio-health activity of the food to be tested.

1. Sports ability: crawling ability

principle:

In this experiment, after feeding the control medium 111 and the food medium 112 containing the food to be tested, the crawling ability test was used to observe whether feeding the food to be tested affected the ability of the fruit fly to move (Nichols et al. , 2012, Gargano et al) . , 2005).

Implementation method:

About 30-100 wild-type Drosophila were reared as Drosophila unit 120 in a breeding tank 110 at 29 ° C, and divided into a control group fed with control medium 111 and a food group to be tested 112 of the food medium to be tested, at 1 to After 2 weeks, the fruit fly unit 120 was moved into the physiological detection component 130 to test the crawling ability.

The physiological detecting component 130 of the embodiment is a plastic culture tube with a scale, and the fruit fly of the Drosophila unit 120 is placed in a plastic culture tube, and tested three times every 18 seconds, and crawls more than 5 cm. Drosophila, which is included in the fruit flies with crawling ability, counts the number of flies that have creeping ability in each group to evaluate the exercise ability of the food medium 112 to be fed.

2. Drosophila Reproductive Capacity Test

Implementation method:

About 5-10 males and 5-10 female virgin flies of each group were reared as the Drosophila unit 120 in the breeding tank 110, and divided into a control group fed with the control medium 111 and a food group to be tested of the food medium 112 to be tested. Check the number of ovulations every day for 10 consecutive days.

The physiological detecting component 130 of the present embodiment may be, for example, an egg counting plate to count the number of ovulations of the fruit fly unit 120, and compare the number of ovulations of the control group and the food group to be tested to evaluate the reproduction of the food medium 112 to be fed. ability. However, the reproductive ability test of the present invention is not limited to the above, and the reproductive ability of the offspring of the Drosophila unit 120 fed the food medium 112 to be tested may be detected, or the male and female flies may be separately fed for a predetermined time, for example, 1, 2, 2, After 3, 4, 5, 10, 15, 20, and 25 days, the fertility test was conducted.

3. Hunger tolerance test

principle:

Hunger tolerance is an important feature of insect survival in the wild. In a variety of animal models, including fruit flies, nematodes, yeast, mice, etc., it has been found that proper hunger can greatly extend the lifespan of animals. Therefore, testing for hunger tolerance in Drosophila has become an important and easy-to-use test.

Implementation method:

Each group of about 3 to 35 male 3 to 5 day adult fruit flies is reared as a Drosophila unit 120 in the breeding tank 110, and divided into a control group fed with the control medium 111 and a food group to be tested of the food medium 112 to be tested. Drosophila unit 120 was moved into physiological detection component 130 after 1 to 2 weeks to test for hunger tolerance.

The physiological detection module 130 of this example was a culture tube containing 10 ml of 1.5% agar and 5 mL of water (nutrient-free medium). The number of deaths of the Drosophila unit 120 was checked daily until the Drosophila unit 120 died, and the average lifespan of the control group and the food group to be tested was compared to evaluate the hunger tolerance of the fed food medium 112 to be tested.

4. Oxidation pressure: Bara (Paraquat) test

principle:

1,1'-dimethyl-4,4'-bipyridinium dichloride (Pq ²⁺ )) from Xingnong Chemical Co., Ltd. Limited. In this experiment, as a pressure source for fruit fly against oxidative stress, it is an indicator of redox. When the samarium enters the cell, Redox cycling occurs, causing oxidation of NADPH and formation of Paraquat radicals. The paraben free radical reoxidizes the oxygen molecule and restores the cationic state, producing a toxic peroxide ion (Superoxide). Peroxygen ions directly destroy the cell's inner membrane and organelles, eventually forming cell death. And because of the reoxidation of the parabens, it will over-consume the NADPH in the cells, which will also promote cell peroxidation and go to cell death (Sampayo et al. , 2003, Suntres, 2002).

Implementation method:

About 100 wild type Drosophila per group were reared as Drosophila unit 120 in a breeding tank 110 at 29 ° C, and divided into a control group fed with control medium 111 and a food group to be tested 112 of the food medium to be tested, at 1 to After 2 weeks, the Drosophila unit 120 was moved into the physiological detection component 130 for an oxidative stress test. Before moving into the physiological detection component 130, the fruit fly of the Drosophila unit 120 is starved for 2 hours to prevent food in the digestive tract from interfering with the test results.

The physiological detection module 130 of the present embodiment is a culture tube containing a mixture of 10 μM of balaquinium and 5% glucose solution. The number of deaths of the Drosophila unit 120 was checked every 3 to 8 hours until the Drosophila unit 120 died, and the average lifespan of the control group and the food group to be tested was compared to evaluate the oxidative stress test of the food medium 112 to be fed.

5. Oxidation pressure: hydrogen peroxide (H ₂ O ₂ ) test

principle:

H ₂ O ₂ (from Union Chemicals Co., Ltd.) is also used here as a source of stress against oxidative stress in fruit flies. Ingestion of H ₂ O ₂ causes ROS in the living body (which is a chemically active oxygen-containing atom or atomic group, and generally contains a radical "superoxide anion O2 ̇ ^- ), hydroxyl radical (hydroxyl radical) ̇OH), lipid peroxide (LIP), and non-radical "hydrogen peroxide (H ₂ O ₂ ), singlet oxygen ¹ O ₂ , etc.), which are active in nature and have The ability to oxidize other substances causes damage to the cells of the organism, thus causing an increase in oxidative stress in the body (Cruz et al. , 2007).

Implementation method:

About 100 wild type Drosophila per group were reared as Drosophila unit 120 in a breeding tank 110 at 29 ° C, and divided into a control group fed with control medium 111 and a food group to be tested 112 of the food medium to be tested, at 1 to After 2 weeks, the Drosophila unit 120 was moved into the physiological detection component 130 for an oxidative stress test. Before moving into the physiological detection component 130, the fruit fly of the Drosophila unit 120 is starved for 2 hours to prevent food in the digestive tract from interfering with the test results.

The physiological detection module 130 of the present embodiment is a culture tube containing a mixed solution of 30% H ₂ O ₂ and 5% glucose solution. The number of deaths of the Drosophila unit 120 was checked every 3 to 8 hours until the Drosophila unit 120 died, and the average lifespan of the control group and the food group to be tested was compared to evaluate the oxidative stress test of the food medium 112 to be fed.

6. Drosophila life observation

Implementation method:

Each group of about 100 identical strains of Drosophila were reared as Drosophila unit 120 in the breeding tank 110 under the same conditions (12 hours light, 12 hours dark day and night cycle and about 60% humidity environment), and divided into feeding controls. The control group of the medium 111 and the food group to be tested of the food medium 112 to be tested are continuously reared until the fruit fly unit 120 is completely dead, and the number of days and the number of days in which the fruit fly unit 120 survives is recorded daily, thereby comparing the control group and the food group to be tested. Statistical differences between mean lifespan and 50% lifespan (Li et al. , 2008).

7. Drosophila weight test

principle:

Drosophila experiments have found that high-carbohydrate and high-fat diets can increase the weight of fruit flies. The most commonly used is a 10-20% high-fat diet, preferably a 15-20% high-fat diet, and a fruit fly weight gain of more than 20% can be observed in 1 to 2 weeks. It can be observed whether the food to be tested has the effect of reducing body weight. If you want to test whether the food to be tested has the effect of increasing body weight, we will use food close to a high-fat diet (10% fat). In this case, the weight of the fruit fly will not increase within two weeks. The ratio of the food to be tested shows whether the food has the effect of increasing body weight.

Implementation method:

Each group of about 20 male 3 to 5 day adult fruit flies was reared as a Drosophila unit 120 in a rearing tank 110, and divided into a control group fed with a control medium 111 containing 10-20% fat and containing 10-20% fat. + The food group to be tested of the food medium 112 to be tested for food to be tested is transferred to the physiological test component 130 before the feeding and after 1 to 3 weeks of feeding to perform the body weight test.

The physiological detecting component 130 of this embodiment is a precision balance. The body weight of the Drosophila unit 120 was separately weighed using a highly sensitive balance, and the body weights of the control group and the food group to be tested were compared to evaluate whether the feeding of the food medium 112 to be tested had an effect of increasing body weight.

8. Analysis of Drosophila Learning and Memory Ability

Implementation method:

The wild type Drosophila of Canton S (CS) strain was reared as Drosophila unit 120 in a breeding tank 110 at 25 ° C and 55% humidity, and divided into a control group fed with control medium 111 and a food to be tested 112. In the group, the Drosophila unit 120 is moved to the physiological testing component 130 after 10 days for learning and memory testing.

Referring to FIG. 2, the physiological detecting component 130 of the present embodiment is a memory testing device 300. The memory test device 300 includes a training tube 310 disposed on the upper portion of the memory test device 300 and a test tube 320 disposed on the lower portion of the memory test device 300. The training tube 310 is a hollow tube body, and has a vent hole 311 at one end and an electric shock portion (not shown) provided on the hollow tube body to apply at least one of an odor and an electric shock to the training tube 310. The test tube 320 is a hollow tube body, and has vent holes 321 and 322 at both ends to respectively apply odor to the test tube. First, the Drosophila unit 120 that has been fed with the control medium 111 or the food medium 112 to be tested is placed in the training tube 310, and the first scent 331 is applied to the Drosophila unit 120 via the vent 311, and then shocked for 1 minute to enable the fruit. The fly unit 120 remembers that the first scent 331 will be subjected to an electric shock, and after 45 seconds, the second scent 332 is administered via the vent 311 without a shock for 1 minute. Next, the trained fruit fly unit 120 is placed in the middle of the test tube 320, and the first scent 331 is applied via the vent 311 and the second odor is applied at the other end to observe the moving direction of the fruit fly unit 120 in the test tube. The number of Drosophila units 120 at both ends was counted to evaluate the effect of feeding each test food medium 112 on memory and learning ability.

In addition, the test method can be further applied to the detection of short-term, medium-term or long-term memory ability of the fruit fly, for example, 3 to 5 hours after the training tube 310 is trained, and then tested in the test tube 320 to evaluate its short-term memory ability; The test tube 320 is tested at 8 to 24 hours after the training tube 310 is trained to evaluate the mid-term memory ability; and more than 24 hours after the training tube 310 is trained (and then tested in the test tube 320 to evaluate its long-term Memory capacity.

Example

1. Exercise ability test

1.1. Effects of organic, non-organic and genetically modified foods on the motor ability of Drosophila

Genetically modified soybeans, common soybeans (non-genetically modified and non-organic soybeans), organic soybeans, organic mung beans and non-organic mung beans were used as foods to be tested, and added to the standard medium in an amount of 10%. About 30 to 35 births of each group were selected as Drosophila unit 120, and were divided into a control group fed with control medium 111 and a food group to be tested of food medium 112 to be tested. After two weeks of feeding, the exercise capacity (climbing test) was tested on a graduated plastic culture tube.

As a result, as shown in Fig. 3, the Drosophila unit 120 fed with organic soybeans and organic mung beans was significantly more potent than the Drosophila unit 120 fed with non-organic or genetically modified soybeans. However, there was no significant difference between the genetically modified soybeans and the Drosophila unit 120 of the common soybeans. However, the present invention is not limited thereto, and the larvae of Drosophila can also be used as the Drosophila unit 120, and mechanically stimulated to quantify the exercise ability of the Drosophila larvae to evaluate the effect of food on exercise performance.

1.2. The effect of various beverages on the ability of Drosophila

Commercially available beverages of the various J~S groups shown in Table 1 below were added to the standard medium in an amount of 40% as the food medium 112 to be tested. About 30 to 35 male flies of 3 to 5 days of each group were selected as Drosophila unit 120, and divided into a control group fed with control medium 111 and a food group to be tested of food medium 112 to be tested. After 21 days of feeding, the exercise capacity (climbing test) was tested on a graduated plastic culture tube. Among them, the nutritional content of each commercially available beverage is shown in Table 1 below: [Table 1]  <TABLE border="1" borderColor="#000000" width="85%"><TBODY><tr><td> Group </td><td> Name </td><td> Heat (g) </td><td> Protein (g) </td><td> Fat (g) </td><td> Saturated fat (g) </td><td> Trans fat (g) </td ><td> Carbohydrate (g) </td><td> Sodium (mg) </td><td> Caffeine</td><td> Ingredients</td></tr><tr><td > J </td><td> Original Japanese Green Tea</td><td> 0 </td><td> 0 </td><td> 0 </td><td> 0 </td> <td> 0 </td><td> 0 </td><td> 8.9 </td><td> 20mg or less</td><td> water, tea extract (green tea), vitamin c sodium salt, Matcha, sodium bicarbonate</td></tr><tr><td> K </td><td> original extract oolong tea</td><td> 0 </td><td> 0 </td> <td> 0 </td><td> 0 </td><td> 0 </td><td> 0 </td><td> 6.9 </td><td> 20mg or less</td>< Td> water, tea extract (oolong tea), vitamin C sodium salt, finely ground oolong tea, sodium bicarbonate</td></tr><tr><td> L </td><td> Japanese Green Tea</td><td> 0 </td><td> 0 </td><td> 0 </td><td> 0 </td><td> 0 </td><td> 0 </td><td> 10 </td><td> 20mg or less</td><td> water, tea (green tea, Japanese sencha) , antioxidants, baking soda</td></tr><tr><td> M </td><td> KIRIN afternoon black tea-lemon</td><td> 28 </td><td> 0 < /td><td> 0 </td><td> 0 </td><td> 0 </td><td> 7 (sugar 6.6) </td><td> 8 </td><td> 20mg or less</td><td> water, high fructose corn syrup, sugar, black tea, lemon juice, flavoring agent (sodium citrate, citric acid), spices, antioxidants</td></tr><tr>< Td> N </td><td> KIRIN afternoon black tea-original</td><td> 16 </td><td> 0 </td><td> 0 </td><td> 0 </td ><td> 0 </td><td> 4 (sugar 3.6) </td><td> 6 </td><td> 20mg or less</td><td> water, high fructose corn syrup, sugar, Black tea, spices, antioxidants</td></tr><tr><td> O </td><td> KIRIN afternoon black tea-milk tea</td><td> 37 </td><td> 0.5 < /td><td> 0.6 </td><td> 0.4 </td><td> 0 </td><td> 7.8 (sugar 7.6) </td><td> 28 </td><td> 20mg or less</td><td> water, milk, sugar, black tea, whole milk powder, skimmed milk powder, maltodextrin, salt, emulsifier (glyceryl succinate, sucrose ester), perfume, antioxidants </ Td></tr><tr><td> P </td><td> Uniform Maixiang carefully selected Wuhe tea </td><td> 40.4 </td><td> 0.4 </td><td> 0.8 </td><td> 0.7 </td><td> 0 </td><td> 7.9 </ Td><td> 12 </td><td> 20mg or less</td><td> water, sucrose, creamer, milk powder, black tea, spices, emulsifier (fatty acid glycerides, mono- and di-fatty acid glycerol Barium tartrate, fatty acid sucrose ester, sodium erythorbate, sodium bicarbonate, barley extract </td></tr><tr><td> Q </td><td> Weidan heart tea road winter melon tea</td ><td> 28 </td><td> 0 </td><td> 0 </td><td> - </td><td> - </td><td> 7.1 (sugar 7.1) < /td><td> 11 </td><td> - </td><td> water, melon juice, sucrose, iso-habitat oligosaccharides, spices (containing propylene glycol, water, caramel, D-sorbitol 70% liquid, ethanol and edible yellow No.5), glycine, caramel, salt</td></tr><tr><td> R </td><td> Kaixi frozen top oolong tea</ Td><td> 12 </td><td> 0 </td><td> 0 </td><td> 0 </td><td> 0 </td><td> 3.1 (sugar 2.9) </td><td> 12 </td><td> 20mg or less</td><td> water, sucrose, oolong tea, antioxidants, salt</td></tr><tr><td> S < /td><td> Yakult live bacteria fermented milk</td><td> 72 </td><td> 1.2 </td>< Td> 0 </td><td> 0 </td><td> 0 </td><td> 16.1 (sugar 13.6) </td><td> 20 </td><td> - </td ><td> Water, sugar sugar, fructose sugar, raw milk, skim milk powder, spices, Lactobacillus casei Shirota, non-fat milk solids 3.0% or more, less than 4.0%, milk fat 0.5% or less </td></tr></TBODY></TABLE> Note: Nutritional ingredients are expressed in terms of content per 100ml. As shown in Figure 4, the fruit fly unit 120 fed with various beverages has significantly lower exercise capacity. Drosophila unit 120 in the control group.  

2. Reproductive test: the effect of organic or non-organic foods on the reproductive capacity of Drosophila

The organic, non-organic food was added to the standard medium in an amount of 10% to serve as the food medium 112 to be tested. About 5 males and 5 female virgin flies of each group were reared as the Drosophila unit 120 in the rearing tank 110, and divided into a control group fed with the control medium 111 and a food group to be tested of the food medium 112 to be tested, from the second Days began to use the eggs count board to calculate the number of eggs laid by fruit flies until the 10th day. As a result, as shown in Fig. 5, the fruit fly unit 120 fed with organic or non-organic soybeans or corn had no significant difference in reproductive ability.

3. Hunger tolerance test

3.1. Effects of organic, non-organic and GM foods on hunger tolerance in Drosophila

The organic, non-organic or non-genetically modified food was added to the standard medium in an amount of 10% to serve as the food medium 112 to be tested. About 30 to 35 male flies born in each group of about 3 to 5 days are housed in the breeding tank 110 as the fruit fly unit 120, and are divided into a control group fed with the control medium 111 and a food group to be tested of the food medium 112 to be tested. Drosophila units 120 were transferred to culture tubes containing nutrient-free medium for testing after 1 to 2 weeks.

As shown in Fig. 6, the Drosophila unit 120 fed the organic mung bean was significantly more potent than the Drosophila unit 120 fed the non-organic mung bean; whereas the fruit fly fed the genetically modified corn had a hunger tolerance Drosophila units 120 fed non-genetically modified corn were significantly reduced. It indicated that feeding organic foods enhanced the hunger tolerance of Drosophila, while feeding genetically modified foods had a negative impact on the hunger tolerance of Drosophila.

3.2. Effects of various beverages on hunger tolerance of Drosophila

The commercially available beverages of Groups J to N shown in Table 1 were added to the standard medium in an amount of 40% to be used as the food medium 112 to be tested. About 30 to 35 male flies per group are housed in the breeding tank 110 as the Drosophila unit 120, and are divided into a control group fed with the control medium 111 and a food group to be tested for the food medium 112 to be tested, and the fruit is obtained after 30 days. The fly unit 120 was transferred to a culture tube containing a nutrient-free medium for testing.

As shown in Figure 7, the commercial beverages fed to Group J reduced the tolerance of Drosophila to hunger; while the commercial beverages fed Groups M and N enhanced the hunger tolerance of Drosophila.

4. Effects of organic, non-organic and genetically modified foods on the resistance of Drosophila to oxidative stress in hydrogen peroxide

The organic, non-organic or genetically modified food was added to the standard medium in an amount of 10% to serve as the food medium 112 to be tested. About 30 to 35 male drosophila of 3 to 5 days of each group are housed as a Drosophila unit 120 in the rearing tank 110, and are divided into a control group fed with the control medium 111 and a food group to be tested of the food medium 112 to be tested. After 2 weeks, the Drosophila unit 120 was transferred to a culture tube containing hydrogen peroxide to carry out tolerance to hydrogen peroxide oxidation pressure.

As a result, as shown in Fig. 8, the Drosophila unit 120 fed the organic black bean was significantly more resistant to the oxidative stress of hydrogen peroxide than the Drosophila unit 120 fed the non-organic black bean; and the Drosophila unit 120 fed the genetically modified corn, Its tolerance to oxidative stress of hydrogen peroxide is significantly lower than that of Drosophila unit 120 fed non-genetically modified corn. It indicated that feeding organic foods has an enhanced ability to resist the antioxidant stress of Drosophila, while feeding genetically modified foods has a negative impact on the antioxidant capacity of Drosophila.

5. Anti-para Oxidative pressure: the effect of various beverages on the ability of Drosophila to resist oxidative stress

Each of the commercially available beverages of the K group to the P group shown in Table 1 was added to the standard medium in an amount of 40% to be used as the food medium 112 to be tested. About 30 to 35 male flies born in each group of about 3 to 5 days are housed in the breeding tank 110 as the fruit fly unit 120, and are divided into a control group fed with the control medium 111 and a food group to be tested of the food medium 112 to be tested. After 17 days, the Drosophila unit 120 was transferred to a culture tube containing arborescens to carry out tolerance to the argon oxidation pressure. As shown in Fig. 9, after feeding the food medium 112 to be tested containing the K group to the P group beverage, the tolerance of the Drosophila unit 120 to the oxidative stress of the arborescens was enhanced, and the L group and The effect of the N group was the most significant.

6. Fruit fly life observation experiment: the effect of coffee on the lifespan of fruit flies

Different concentrations of coffee (2.5-20 mg/ml) were added to the standard medium in an amount of 10% to serve as the food medium 112 to be tested. About 30 to 35 male drosophila of 3 to 5 days of each group are housed as a Drosophila unit 120 in the rearing tank 110, and are divided into a control group fed with the control medium 111 and a food group to be tested of the food medium 112 to be tested. The number of deaths of the fruit fly unit 120 was continuously maintained and recorded every day until the fruit fly unit 120 died.

As shown in Figure 10. According to the experimental results, different concentrations of coffee have no negative impact on the lifespan of fruit flies. In view of the fact that caffeine itself has an insecticidal effect at high concentrations, while the high concentration of coffee (2.5 mg/mL is equivalent to 1 cup of coffee; 20 mg/mL is equivalent to 8 cups of coffee per day), the life of the fruit fly is still There is no negative impact, indicating that other ingredients in the coffee may neutralize the insecticidal effects of caffeine.

7. Drosophila weight observation experiment

7.1. The effect of various beverages on the weight of fruit flies

The commercially available beverages of Groups J to S shown in Table 1 were added to the standard medium in an amount of 40% as the food medium 112 to be tested. About 30 to 35 male flies born in each group of about 3 to 5 days are housed in the breeding tank 110 as the fruit fly unit 120, and are divided into a control group fed with the control medium 111 and a food group to be tested of the food medium 112 to be tested. The Drosophila unit 120 was weighed with a precision balance before feeding and after 3 weeks to evaluate the change in body weight of each of the food medium 112 to be tested. As a result, as shown in Fig. 11, after the partial beverage was fed, the average body weight of the fruit fly unit 120 was increased, and the effects of the L group and the M group were the most significant.

7.2. Effect of coffee on weight gain caused by high fat food

Add different doses of high quality coffee (as shown in Figure 12, equivalent to the daily number of cups consumed by humans) or caffeine 0.5 mg (equivalent to 4 to 5 cups of coffee per day) to a standard medium containing 20% coconut oil. The medium was used as the food medium 112 to be tested, and the standard medium and the standard medium containing 20% coconut oil were used as the control medium 111. About 30 to 35 male flies born in each group of about 3 to 5 days are housed in the breeding tank 110 as the fruit fly unit 120, and are divided into a control group fed with the control medium 111 and a food group to be tested of the food medium 112 to be tested. The Drosophila unit 120 was weighed with a precision balance before feeding and after 2 weeks to evaluate the change in body weight of each of the food medium 112 to be tested. However, this creation is not limited to this, and it is also possible to determine the fat content of fruit flies to assess the effect of food on body fat increase and decrease.

The result is shown in Fig. 12. Feeding the control medium 111 containing 20% fat significantly increased the average body weight of the Drosophila unit 120, while feeding the test food medium 112 containing different concentrations of coffee completely resisted the weight gain of the high fat food. In addition, the test food medium 112 containing 0.5 mg of caffeine can also counteract the weight gain of the fruit fly unit 120, but only partially. The effect of feeding coffee is significantly better than that of feeding caffeine, indicating that ingredients other than caffeine in coffee also have a weight-increasing effect against high-fat foods.

8. Drosophila learning memory test : The memory of coffee on the fruit fly that can restore sleep deprivation

Different concentrations of coffee were added to the standard medium as the food medium 112 to be tested. About 30 to 35 male drosophila of 3 to 5 days of each group are housed as a Drosophila unit 120 in the rearing tank 110, and are divided into a control group fed with the control medium 111 and a food group to be tested of the food medium 112 to be tested. One week after the feeding, the sleep deprivation device (not shown) was used to deprive the fruit fly for one night, and then the memory test device 300 was used to test the 3-hour memory ability of the Drosophila unit 120 of the food group to be tested and the control group.

The results are shown in Figure 13, after sleep deprivation, the Drosophila unit 120 fed the control medium 111 but undergoing sleep deprivation has significantly lower memory than the Drosophila unit 120 fed the control medium 111 but not deprived of sleep, indicating that lack of sleep leads to fruit flies Memory decline. In addition, the Drosophila unit 120 that feeds coffee, despite sleep deprivation, has a memory similar to that of the Drosophila unit 120 that is not deprived of sleep, indicating that coffee can restore memory loss in Drosophila caused by sleep deprivation.

Based on the above results, the system of this creation can effectively test the effects of various foods and beverages on the health or vitality of fruit flies. For example, some foods or beverages have a negative impact on the health of fruit flies, and some genetically modified foods can reduce the fruit fly. The ability of hunger tolerance or anti-oxidative stress, and some beverages can reduce the anti-oxidative stress of Drosophila or have the negative effect of increasing body weight, thus showing that the system according to the present invention can effectively achieve food safety for detecting the food or beverage. The effect of sex.

On the other hand, some foods have an enhanced effect on the health or vitality of fruit flies. Some organic foods can enhance the ability of fruit flies to exercise or resist oxidative stress; some beverages can also increase the antioxidant capacity of fruit flies; Coffee and caffeine have a weight-increasing effect against high-fat foods, and can completely restore the memory decline of sleep-deficient fruit flies, thereby indicating that the system according to the present invention can effectively achieve the effect of identifying the bio-health activity of the food or beverage.

According to the system of this creation, life indicators can be measured in a short period of time, even including the function of the advanced nervous system and the measurement of fertility, as well as the effects of long-term consumption. Furthermore, the system of this creation can be further extended to detect the addition of various food additives such as food coloring, leavening agents, preservatives, sweeteners and antioxidants. That is to say, various types of additives in their respective safety ranges are often added to foods at the same time, and the system of the present invention can be used for food safety testing of foods with several additives. Furthermore, the system of the present invention can also be applied to the detection of food safety or bio-health activity of various crops or wild foods, seafood and artificially manufactured foods and drinks grown in different environments.

The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Any of the above-described embodiments may be modified or changed without departing from the technical spirit and spirit of the present invention. Therefore, the scope of protection of this creation should be as listed in the scope of the patent application described later.

100‧‧‧ system
110‧‧‧ breeding tank
111‧‧‧Control medium
112‧‧‧Food medium to be tested
120‧‧‧Drosophila unit
130‧‧‧Physiological testing components
300‧‧‧ memory test device
310‧‧‧ training tube
311, 321, 322‧‧ vents
320‧‧‧Test tube
331‧‧‧ first smell
332‧‧‧second smell

Figure 1 is a schematic representation of a system for detecting food safety or biohealth activity.

Figure 2 is an image of a memory test device for fruit flies.

Figure 3 is a graph of exercise performance testing after feeding with or without genetic modification or organic or non-organic food (feeding for 14 days).

Figure 4 is a graph of the 21-day flies exercise ability test with various types of beverages.

Figure 5 is a graph showing the effect of feeding organic or non-organic foods on the reproductive capacity of Drosophila.

Figure 6 is a graph of the hunger tolerance test after two weeks of feeding organic or non-organic or GM foods.

Figure 7 is a graph showing the effect of feeding various beverages on hunger tolerance in Drosophila.

Figure 8 is a graph of the oxidative stress test of Drosophila against hydrogen peroxide (H ₂ O ₂ ) after two weeks of feeding organic or non-organic or GM foods.

Figure 9 is a graph showing the oxidative stress test of Drosophila anti-Balatin by feeding various beverages.

Figure 10 is a graph showing the effect of feeding different doses of coffee on the lifespan of Drosophila.

Figure 11 is a graph of the effects of various types of drinks (three weeks of feeding) on the weight of fruit flies.

Figure 12 is a graph showing the effect of weight gain on high-fat foods after two weeks of feeding coffee and caffeine.

Figure 13 is a graph of the effect of feeding coffee on the memory of Drosophila in sleep deprivation (sleep deprivation).

Claims (15)

A system for food safety or bio-health activity, comprising: at least two breeding tanks each having at least one side wall and a bottom, the side wall surrounding and joining the bottom, the side wall and the bottom defining an accommodating space, The accommodating space is respectively placed in a control medium and a food medium containing one food to be tested; at least two fruit fly units comprising a fruit fly, and respectively placed in the accommodating space containing the control medium and containing the same The accommodating space of the food medium to be tested is fed to the fruit fly for a predetermined time; and a physiological detecting component is disposed outside the at least two breeding tanks, and the fruit fly unit feeding the control medium is fed and fed Measuring the Drosophila unit of the food medium into the physiological detecting component to perform a test item, and comparing the test data of the Drosophila unit fed the control medium and the test food medium to obtain a result, wherein the result is used To assess the food safety or bio-health activity of the food to be tested. The system of claim 1, wherein when detecting the food safety of the food to be tested, the test item comprises exercise capacity, reproductive ability, starvation tolerance test, oxidative stress test, life observation and memory analysis. At least one of them. The system of claim 1, wherein when detecting the bio-health activity of the food to be tested, the test item comprises exercise capacity, fertility, starvation tolerance test, oxidative stress test, life observation, body weight test, and At least one of the memory analyses. The system of claim 2, wherein the physiological detection component is a culture tube having a scale to determine that the Drosophila unit is in the culture tube when the exercise capability is detected. The distance to climb or fly up. The system of claim 2, wherein the physiological detection component is a counting device for counting the number of ovulations of the fruit fly unit when detecting the reproductive capacity. The system of claim 5, wherein when the fertility is detected, the counting device is used to count the number of ovulations of the offspring of the Drosophila unit. The system of claim 2, wherein the physiological testing component is a culture tube containing agar and water, and the fruit fly is recorded at each predetermined time when the starvation tolerance test is performed. The number of deaths in the unit. The system of claim 2, wherein the physiological testing component is a culture tube containing a mixture of hydrogen peroxide or balazone and a glucose solution when the oxidation stress test is performed, and The number of deaths of the fruit fly unit is recorded at a predetermined time. The system of claim 2, wherein the physiological testing component is a memory testing device, and the memory testing device comprises: a training tube, which is hollow a tube body having a vent hole at one end and an electric shock portion disposed on the hollow tube body to apply at least one of an odor and an electric shock to the training tube; and a test tube, which is a hollow tube body, and two The ends each have a venting hole for applying an odor to the test tube; wherein the Drosophila unit is first placed in the training tube, and the Drosophila unit is subjected to an electric shock and a first scent, after a predetermined time Applying a second scent without an electric shock, then placing the trained Drosophila unit in the middle of the test tube and applying the first scent at one end of the test tube and at the other end The second scent is administered to determine the memory capacity by the Drosophila unit in the direction of movement of the test tube. The system of claim 3, wherein when the weight test is performed, the physiological test component is a weighing device, and the weight of the Drosophila unit is weighed by the weighing device. The system of claim 1, wherein the fruit fly is a wild type fruit fly or a fruit fly used in a laboratory. The system of claim 1, wherein the fruit fly is a Drosophila adult or a Drosophila larva. The system of claim 1, wherein the food to be tested is a food that can be eaten or consumed or a beverage that can be eaten or consumed. The system of claim 1, wherein the food to be tested is an organic food. The system of claim 1, wherein the food medium to be tested contains one or more foods to be tested.