TWM546987U - Structure of high-efficiency mRNA marker detection reagent set - Google Patents

Description

High-performance mRNA marker detection reagent group structure

This creation is about a high-performance mRNA marker detection reagent group structure, especially one that does not include PCR nucleic acid amplification technology, and integrates RNA reverse transcription reaction with biotin calibration as a step, especially referring to the innovative formula and Repeat the reaction to achieve the corresponding effect.

Analysis of gene overexpression has led to fundamental advances and clinical advances in disease diagnosis. The techniques for studying gene transition performance include Northern Blot, Reverse Transcription Polymerase Chain Reaction (RT-PCR), and Real-Time Polymerization Chain Chain Reaction (Real-Time PCR). ). Among them, Northern Blot is very cumbersome in operation and requires too much sample. Therefore, it is limited to research operations and cannot be practically applied to clinical diagnosis. RT-PCR and Real-Time PCR are widely used in the application of single gene detection, such as the detection of hepatitis virus and the identification of infectious pathogens. However, although the creation of PCR sequences is the greatest experiment in the whole, most PCR technologies still have specific problems in common. The main problems include: firstly, false positives for pollution, transitional sensitive reconnaissance, such as foggy DNA or previous The sample residue; secondly, RT-PCR is only considered to be semi-quantitative, because it is difficult to control the efficiency of sequence amplification when comparing different samples; further, due to the interference between the required primers (Annearling), Regardless of whether RT-PCR or Real-Time PCR is widely used for the detection of a single gene target, when the target is a gene group, PCR-related techniques have disadvantages such as time-consuming, labor-intensive, and high-cost operation. With the rapid development of biotechnology in recent years, the application of biochips in clinical medical diagnosis or efficacy evaluation has been gradually taken seriously. Previous studies of the present invention have developed and evaluated a Membrane Array method which can be used for Cancer diagnosis, simultaneous detection of the performance of a variety of mRNA-labeled primers in a blood sample (Peripheral Blood). The molecular signature was evaluated by RT-PCR and nylon membrane wafers. The data was obtained from RT-PCR and nylon membrane wafers and was subjected to linear regression analysis, showing a high correlation between the results of the two methods (r=0.979 , P < 0.0001). In addition, Weighted Chemiluminescent Membrane Array (WCHMA), a related derivative technique, is used for the K-ras of the target of the Target Therapeutic Drug for Lung Cancer patients. Abnormal situations have also been accepted for publication in lung cancer journals. Although many applications of nylon membrane wafers for molecular diagnostics and pharmacodynamic evaluation have been made, however, due to the use of the original nylon membrane wafers, the concept of each gene is equivalent to the importance of a particular disease. The detection specificity is not easy to increase after reaching a certain level. In this regard, the present invention additionally develops a gene group detection structure, and although the biological sample detection and analysis can be performed in a large amount and quickly, the detection process needs to be amplified first, and the blood can be detected directly from the blood test through the gene chip. Gene performance, resulting in wafer detection sensitivity and convenience, is still insufficient. Therefore, the present invention further develops an enzyme-type gene wafer detection reagent group structure, and although it is not necessary to perform amplification, the gene expression in the blood can be detected directly from the blood detection through the gene chip, but it includes RNA extraction and cDNA. Complex processing steps such as synthesis, probe labeling, hybridization reaction, and signal detection, and the hybridization reaction is only one-stage temperature control, and the reaction time is long (requires at least 6.4 hours or more); therefore, overall, the technology The time required for detection is lengthy and consumes a lot of solvent cleaning (six-stage cleaning step), resulting in increased cost and reduced efficiency and efficiency. The background value of the output signal is also higher, and the signal is more susceptible to distortion. Therefore, the user-like users cannot meet the needs of the user in actual use.

The main purpose of this creation is to overcome the above problems encountered in the prior art and to provide a technique that does not include PCR nucleic acid amplification, and integrates RNA reverse transcription reaction with biotin calibration into one step, and then through innovative formula and repeated reaction. It can detect the gene expression in the sample through the gene chip without the need for amplification during the clinical examination. It has high sensitivity and better convenience, and can meet the high sensitivity and convenience of high performance at one time. The structure of the mRNA marker detection reagent group. For the purpose of the above, the present invention is a high-performance mRNA marker detection reagent group structure, which comprises: a gene detector comprising: a gene chip sensing unit, which is provided with a purification and calibration area of the sample to be tested and A gene wafer reaction and imaging zone, the gene wafer reaction and the imaging zone are provided with a gene chip, which selects an oligonucleic acid sulphate fragment and is used as a housekeeper based on the quality control of the gene chip reaction. a housekeeping gene, which affixes an oligo-nuclear acid fragment containing a plurality of target genes to a wafer of a thermoplastic material to form a labeled specific sequence on the gene wafer; a gene wafer reaction reagent unit Connected to the gene chip sensing unit for providing all the reagents required for the reaction of the gene wafer, comprising an RNA extraction component, a biotin calibration component, a hybrid reaction component, and a signal detection component; a unit connected to the gene chip sensing unit; an image capturing unit connected to the gene chip sensing unit; and a management operation unit The gene chip sensing unit, the gene wafer reaction reagent unit, the temperature control unit, and the image capturing unit are connected, and can be controlled as a unit when the unit is operated; and the gene chip sensing unit is purified by the sample to be tested. The calibration zone uses the reagent provided by the gene wafer reaction reagent unit to purify the mRNA in the sample to be tested by magnetic beads, and the mRNA is labeled as a probe by biotin and then transferred to the reaction and imaging area of the gene wafer. Performing a reaction to the reagent provided by the gene wafer reaction reagent unit, and the temperature control unit performs the two-stage temperature control on the gene wafer reaction and the imaging region, and the probe is hybridized with the gene wafer. Then, the heterozygous probe on the gene chip is subjected to color development by adding a signal display agent to perform color detection, and the management operation unit passes the image capturing unit to the gene wafer reaction and imaging area. Taking the image of the probe to be tested and the probe of the gene chip for hybridization and imaging, and controlling the process of gene detection according to a manipulation parameter, control Adding each reagent in the gene reaction unit of the gene, and controlling the output temperature and the number of temperature control times of the temperature control unit; and a controller connected to the gene detector, comprising a processing unit, a processing unit and the processing unit a connected input unit and an output unit connected to the processing unit, through which the user inputs the required manipulation parameters, and the processing unit transmits the manipulation parameters to the management operation unit to control the genetic detection Carrying out the process of gene detection, receiving the image captured by the gene detector, and automatically analyzing the image result, and finally outputting the analysis result by the output unit, the RNA extraction component in the gene chip reaction reagent unit The invention comprises a protein □ reagent, a specific cleavage reagent for RNA, a magnetic bead preservation/binding reagent, an RNA cleaning reagent and an RNA resolving/preserving reagent; the biotin calibration component comprises a biotin calibration reagent and a biotin immunobinding reagent; the hybrid reaction component Containing a hybrid reaction reagent; and the signal detecting component includes a buffer cleaning reagent, miscellaneous Signal blocking reagents and signal display agents, a total of twelve reagents involved in the reaction. In the above embodiment of the present invention, the thermoplastic material is polypropylene (PP) or polymethyl Methacrylate (PMMA). In the above examples, the oligo-nucleic acid fragment is a synthetic oligo-nucleic acid solution having a concentration ranging from 10 uM to 200 uM, a sequence length of 40 to 60 bases, and having a target gene. The special sequence presents a complementary relationship. In the above examples, the protein □ reagent is a group consisting of protein K (Protease K) and a preservation solution. In the above examples of the present invention, the RNA-specific lysis reagent is a group consisting of a deoxyribonuclease (DNase), a lysis formulation, and an RNA protection formulation. In the above embodiments of the present invention, the magnetic bead retention/binding reagent is a group consisting of magnetic beads, a nucleic acid binding formulation, and an RNA protection formulation. In the above examples of the present creation, the RNA cleaning reagent is a group consisting of a detergent and an RNA protection formulation. In the above examples of the present creation, the RNA re-dissolving/preserving reagent is an RNA preservation solution. In the above embodiments of the present invention, the biotin calibration reagent is a group consisting of biotin-labeled nucleic acid, nucleic acid material, nucleic acid polymerization, primer, and antifreeze. In the above examples of the present invention, the biotin immunobinding reagent is a group consisting of biotin immuno-antibody, phosphoric acid, and antifreeze. In the above examples of the present creation, the hybrid reaction reagent is a group consisting of a heterozygous reaction formulation, a background inhibiting formulation, a detergent, and an inorganic salt. In the above embodiments of the present invention, the buffer cleaning reagent is a group consisting of a detergent and an inorganic salt. In the above embodiments of the present invention, the noise blocking reagent is a group consisting of a detergent and a background suppression formulation. In the above embodiment of the present invention, the signal display agent is 5-bromo-4-chloro-3-□□phosphoric acid-nitrotetrazolium chloride staining solution (BCIP/NBT Solution). In the above embodiment of the present invention, the gene chip is a housekeeping gene using β-actin and Glyceraldehyde 3-phosphate dehydrogenase (GADPH). In the above embodiment of the present creation, the processing unit has an analysis software and an image capturing mode and a data transmission mode. In the above embodiment of the present creation, the processing unit is a single chip or a programmable logic array. In the above embodiment of the present creation, the input unit is a plurality of buttons. In the above embodiment of the present creation, the output unit is a display screen. In the above embodiment of the present creation, the system to be tested is blood, body fluid, cell culture or tissue cells.

Please refer to the "Figure 1 and Figure 2" diagrams for the basic architecture of the creation and the block diagram of this creation. As shown in the figure: This creation is a high-performance mRNA marker detection reagent group structure, which comprises a gene detector 1 and a controller 2. The gene detector 1 includes a gene chip sensing unit 11, a gene chip reaction reagent unit 12 connected to the gene chip sensing unit 11, and a temperature control unit 13 connected to the gene chip sensing unit 11. An image capturing unit 14 coupled to the gene chip sensing unit 11, and a gene chip sensing unit 11, the gene wafer reaction reagent unit 12, the temperature control unit 13, and the image capturing unit 14 The management operation unit 15 is connected, and the management operation unit 15 is controlled as the operation of each of the units 11 to 14. The gene chip sensing unit 11 is provided with a sample purification and calibration area 111 and a gene wafer reaction and imaging area 112, and the gene wafer reaction and imaging area 112 is provided with a gene chip 3, the gene Wafer 3 selects an oligo-nucleic acid fragment and dehydrogenates it with two housekeeping genes, β-actin and glyceraldehyde 3-phosphate, which are the basis for quality control of the gene chip reaction. □ (Glyceraldehyde 3-phosphate dehydrogenase, GADPH), the oligo-nucleic acid sulphate fragment containing a plurality of target genes is immobilized on a wafer to form a calibrated specific sequence on the gene chip 3, wherein the oligo-acid sulphuric acid fragment It is a synthetic oligonucleic acid solution with a concentration ranging from 10 uM to 200 uM, a sequence length of 40 to 60 bases, and a complementary relationship with a specific sequence on the target gene. The gene wafer reaction reagent unit 12 provides all the reagents required for the reaction of the gene wafer 3, and is composed of an RNA extraction element 121, a biotin calibration element 122, a hybrid reaction element 123, and a signal detection element 124. The RNA extraction element 121 comprises a protein □ reagent, an RNA-specific lysis reagent, a magnetic bead preservation/binding reagent, an RNA cleaning reagent, and an RNA re-dissolving/preserving reagent; the biotin calibration component 122 comprises a biotin calibration reagent and biotin immunoassay. The hybrid reaction element 123 comprises a hybrid reaction reagent; and the signal detection element 124 comprises a buffer cleaning reagent, a noise blocking reagent and a signal display agent, and a total of twelve reagents participate in the reaction. The controller 2 is connected to the gene detector 1 for control, reaction image analysis and data display during operation of the gene detector 1, and the controller 2 includes a processing unit 21, a processing unit and the processing unit An input unit 22 connected to the processing unit 21 and an output unit 23 connected to the processing unit 21, wherein the processing unit 21 can be a single chip or a programmable logic array, and has an analysis software and an image capturing mode and a data transmission mode. The input unit 22 can be a plurality of buttons, and the output unit 23 can be a display screen. As such, a novel high performance mRNA marker detection reagent set structure is constructed by the above disclosed structure. The above-mentioned gene wafer 3 may be a material such as a Nylon membrane or a thermoplastic material to which a core acid is attached, wherein the thermoplastic material may be polypropylene (PP) or polymethyl methacrylate. (Polymethyl Methacrylate, PMMA). The gene wafer 3 is distributed on the wafer substrate in an amount of 30 standard liters (nL) or more, and the oligo-nucleic acid fragments are repeatedly and arranged in a conventional manner, and dried by ultraviolet light. It is cured by irradiation. The protein □ reagent of the above RNA extraction element 121 is a group consisting of protein K (Protease K) and a preservation solution; the RNA-specific cleavage reagent is protected by deoxyribonuclease (DNase), lysis formula, and RNA protection. The group consisting of the formula uses the addition of DNase to remove the interference of DNA molecules; the magnetic bead preservation/binding reagent is composed of magnetic beads, nucleic acid binding formula, and RNA protection formula, which is extended by adding functional group protection components. The expiration period of the reagent is opened; the RNA cleaning reagent is a group consisting of a detergent and an RNA protection formula, which saves the operation steps in a single formulation, improves efficiency and maintains efficacy; and the RNA resolving/preserving reagent is an RNA preservation solution. Use the addition of RNA protection ingredients to ensure calibration results. This creation has better reaction formula and RNA protection effect than traditional ones. The biotin calibration reagent of the biotin calibration element 122 is a group consisting of biotin-labeled nucleic acid, nucleic acid material, nucleic acid polymerization, primer, and antifreeze; and biotin immuno-binding reagent is biotin-immunized antibody, A group consisting of phosphoric acid □ and antifreeze. This creation is based on an innovative formula for biotin calibration, which eliminates the need for reverse transcription reactions, improves efficiency and maintains performance, and has better calibration efficiency and immunocombination effects than traditional ones. The hybrid reaction reagent of the above heterozygous reaction element 123 is a group consisting of a hybrid reaction formula, a background inhibiting formula, a detergent, and an inorganic salt. This creation uses an innovative formula to shorten the reaction time, improve efficiency and maintain efficiency. Compared with the traditional, it has better hybrid reaction effect, and the background value is lower when the signal is produced. The buffer cleaning reagent of the signal detecting component 124 is composed of a cleaning agent and an inorganic salt, which saves the operation step and improves the efficiency by a single formula; the noise blocking reagent is composed of a cleaning agent and a background suppression formula. Group, with innovative formula to shorten the reaction time, improve efficiency and maintain efficiency; and the signal display agent is 5-bromo-4-chloro-3-□□ phosphoric acid-chlorinated nitrotetrazolium blue staining solution ( BCIP/NBT Solution). This creation has a shorter response time than the traditional one, and the background value is lower when the signal is output, and the signal is not distorted. When used, the gene detector 1 uses the reagent provided by the gene wafer sensing unit 11 in the sample purification and calibration area 111 through the gene wafer reaction reagent unit 12 to purify the sample to be tested by magnetic beads. The mRNA, wherein the system to be tested may be blood, body fluid, cell culture or tissue cells. The mRNA is further labeled as a probe by biotin, and then sent to the gene wafer reaction and the reaction zone 112 for reaction, and the reagent provided by the gene wafer reaction reagent unit 12 is used by the temperature control unit 13 The gene wafer reaction and the imaging area 112 are subjected to two-stage temperature control, and the probe is hybridized with the gene wafer, and the hybrid probe on the gene wafer is added with a signal display agent. Color development, the gene detection is performed, and the management operation unit 15 performs the hybridization of the gene wafer reaction and the imaging area 112 by the image capturing unit 14 to capture the probe and the probe of the gene wafer. The image generated after the reaction and development, and the process of controlling the gene detection according to the control parameter of the controller 2, controlling the addition of each reagent in the reagent reaction unit 12 of the gene, and controlling the output temperature and temperature of the temperature control unit 13 Number of controls. The controller 2 provides the user with the required manipulation parameters through the input unit 22, and the processing unit 21 transmits the manipulation parameters to the management operation unit 15 of the gene detector 1 to control the gene detector 1 for genetic detection. The process receives the image captured by the gene detector 1 and observes the performance of the housekeeping gene according to the completed result to automatically analyze the image result, and convert it into data by electronic processing, and grayscale white matter. In inverse proportion, the analysis result is finally outputted by the output unit 23. In a preferred embodiment, the present invention has the following advantages over the traditional human blood: 1. RNA extraction: using blood as a sample, using DNase to reduce the interference of DNA molecules, the purity can be higher than the conventional one. RNA, and the extracted RNA is produced in a higher yield, which effectively improves the efficiency. 2. Biotin calibration: using RNA as a template for calibration, combining traditional cDNA reverse transcription and probe calibration in two steps to complete the reverse transcription, and to enhance the performance and repeat once (total 2 times) More effective than traditional. 3. Hybrid reaction: the biotin-labeled probe reacts with the gene wafer, and the hybridization reaction time is shortened by the two-stage temperature control (fastest 3 hours), with better binding force and lower background value, which is more effective than the traditional one. Improve efficiency. 4. Signal detection: The cleaning reaction step is reduced, and only has a three-stage cleaning step, so that the signal after the hybrid reaction is generated, which is more efficient than the conventional effective improvement (improved part of the reagent formulation is faster). As can be seen from the above, this creation does not include PCR nucleic acid amplification technology, and integrates RNA reverse transcription reaction with biotin calibration into one step, and then achieves corresponding effects through innovative formula and repeated reaction. The innovation of the biotin calibration reagent formulation lies in the reagents that contain the "reverse transcription" reaction, so there is no need to separate the two steps. The repeated reaction refers to repeated reverse transcription biotin calibration, which can enhance the signal after the hybrid reaction. Therefore, the structure of the high-performance mRNA labeling detection reagent set by the present invention can detect the gene expression in the sample through the gene chip without the need for amplification during the clinical examination, and has high sensitivity and The convenience is better, and the requirement of satisfying high sensitivity and convenience can be achieved once. In summary, this creation is a high-performance mRNA marker detection reagent group structure, which can effectively improve various shortcomings, does not include PCR nucleic acid amplification technology, and integrates RNA reverse transcription reaction with biotin calibration into one step. Through innovative formula and repeated reaction, it can be used for clinical testing, without amplification, and can detect the gene expression in the sample through the gene chip. It has high sensitivity and better convenience, and can meet high sensitivity at one time. The need for convenience, in turn, makes the creation of this creation more progressive, more practical, and more in line with the needs of users. It has indeed met the requirements of the new patent application and has filed a patent application in accordance with the law. However, the above descriptions are only preferred embodiments of the present invention, and the scope of the present invention cannot be limited by this; therefore, the simple equivalent changes and modifications made by the scope of the patent application and the contents of the new manual are All should remain within the scope of this creation patent.

1‧‧‧Gene detector 11‧‧‧Gene chip sensing unit 111‧‧‧Testing object purification and calibration area 112‧‧‧Gene chip reaction and imaging area 12‧‧‧Gene chip reaction reagent unit 121‧ ‧‧RNA extraction element 122‧‧‧Biotin calibration element 123‧‧‧Hybrid response element 124‧‧‧Signal detection element 13‧‧‧Temperature control unit 14‧‧‧Image capture unit 15‧‧‧Management operation Unit 2‧‧‧Controller 21‧‧‧Processing Unit 22‧‧‧ Input Unit 23‧‧‧ Output Unit 3‧‧‧Gene Chip

Figure 1 is a block diagram of the basic architecture of this creation. Figure 2 is a block diagram of the creation.

1‧‧‧Gene detector

11‧‧‧Gene chip sensing unit

111‧‧‧Purification and calibration area of the sample to be tested

112‧‧‧Genetic wafer reaction and imaging area

12‧‧‧Genetic wafer reaction reagent unit

121‧‧‧RNA extraction components

122‧‧‧Biotin calibration components

123‧‧‧Hybrid response elements

124‧‧‧Signal detection component

13‧‧‧temperature control unit

14‧‧‧Image capture unit

15‧‧‧Management unit

2‧‧‧ Controller

21‧‧‧Processing unit

22‧‧‧Input unit

23‧‧‧Output unit

3‧‧‧Gene chip

Claims (20)

A high-performance mRNA labeling detection reagent group structure comprises: a gene detector comprising: a gene chip sensing unit, comprising a sample purification and calibration area and a gene wafer reaction and imaging area; The gene wafer reaction and imaging area is provided with a gene chip, which selects an oligo-acidic acid fragment and uses two housekeeping genes as the basis for controlling the quality of the gene wafer reaction. The oligocore sulphate fragment of the plurality of target genes is immobilized on the thermoplastic material wafer to form a labeled specific sequence on the gene wafer; and a gene wafer reaction reagent unit is connected to the gene chip sensing unit. All reagents required for providing the gene wafer for reaction are composed of an RNA extraction element, a biotin calibration element, a hybrid reaction element and a signal detection element; a temperature control unit is used to sense the gene chip Unit connection; an image capture unit connected to the gene chip sensing unit; and a management operation unit, the gene chip sensing unit, the base The wafer reaction reagent unit, the temperature control unit, and the image capturing unit are connected, and can be controlled as the operation of each unit; the gene chip sensing unit reacts through the gene wafer in the purification and calibration area of the sample to be tested. The reagent provided by the reagent unit purifies the mRNA in the sample to be tested by magnetic beads, and the mRNA is labeled as a probe by biotin, and then sent to the reaction of the gene wafer and the imaging zone for reaction, and the gene chip is used. a reagent provided by the reagent unit, and the temperature control unit performs a two-stage temperature control on the gene wafer reaction and the imaging region, and the probe is hybridized with the gene wafer, and then the gene is wafer-on-chip. The hybrid probe and the signal display agent perform color development to realize gene detection, and the management operation unit uses the image capturing unit to extract the sample to be detected and the imaging area The probe of the gene wafer is subjected to a hybrid reaction and image generated by the image, and the process of detecting the gene is controlled according to a manipulation parameter to control the gene reaction unit of the gene wafer. Adding each reagent, and controlling the output temperature and temperature control number of the temperature control unit; and a controller connected to the gene detector, comprising a processing unit, an input unit connected to the processing unit, and An output unit connected to the processing unit, through which the user inputs the required manipulation parameters, and the processing unit transmits the manipulation parameters to the management operation unit to control the genetic detector to perform the process of detecting the gene And receiving the image captured by the genetic detector, and automatically analyzing the image result, and finally outputting the analysis result by the output unit, wherein the RNA extraction component comprises protein □ reagent, RNA a dedicated lysis reagent, a magnetic bead preservation/binding reagent, an RNA cleaning reagent, and an RNA re-dissolving/preserving reagent; the biotin calibration element comprises a biotin calibration reagent and a biotin immunobinding reagent; the hybrid reaction element comprises a hybrid reaction reagent; And the signal detecting component comprises a buffer cleaning reagent, a noise blocking reagent and a signal display agent A total of twelve reagents participated in the reaction. The structure of the high-performance mRNA labeling detection reagent group according to the first aspect of the patent application, wherein the thermoplastic material is polypropylene (PP) or polymethyl Methacrylate (PMMA). According to the structure of the high-performance mRNA labeling detection reagent group described in claim 1, wherein the oligo-nucleic acid fragment is a synthetic oligonucleic acid solution having a concentration ranging from 10 uM to 200 uM, and the sequence length is It is 40 to 60 bases and has a complementary relationship with a specific sequence on the target gene. The high-performance mRNA labeling detection reagent group structure according to the first aspect of the patent application, wherein the protein □ reagent is a group consisting of protein K (Protease K) and a preservation solution. The high-performance mRNA labeling detection reagent group structure according to the first aspect of the patent application scope, wherein the RNA-specific cleavage reagent is a group consisting of a deoxyribonuclease (DNase), a lysis formula, and an RNA protection formula. group. The high-performance mRNA labeling detection reagent group structure according to the first aspect of the patent application, wherein the magnetic bead storage/binding reagent is a group consisting of a magnetic bead, a nucleic acid binding formulation, and an RNA protection formulation. The high-performance mRNA labeling detection reagent group structure according to the first aspect of the patent application, wherein the RNA cleaning reagent is a group consisting of a detergent and an RNA protection formula. The high-performance mRNA labeling detection reagent group structure according to the first aspect of the patent application, wherein the RNA re-dissolving/preserving reagent is an RNA preservation solution. The high-performance mRNA labeling detection reagent group structure according to the first aspect of the patent application, wherein the biotin calibration reagent is composed of biotin-labeled nucleic acid, nucleic acid material, nucleic acid polymerization, primer, and antifreeze. Group. The high performance mRNA labeling detection reagent set according to the first aspect of the invention, wherein the biotin immunobinding reagent is a group consisting of biotin immuno-antibody, phosphoric acid, and antifreeze. The high-performance mRNA labeling detection reagent group structure according to the first aspect of the patent application, wherein the hybrid reaction reagent is a group consisting of a heterozygous reaction formula, a background suppression formula, a detergent, and an inorganic salt. . The high performance mRNA labeling detection reagent set according to the first aspect of the invention, wherein the buffer cleaning reagent is a group consisting of a detergent and an inorganic salt. The high performance mRNA labeling detection reagent set according to claim 1, wherein the noise blocking reagent is a group consisting of a detergent and a background suppression formula. According to the structure of the high-performance mRNA labeling detection reagent group described in claim 1, wherein the signal display agent is 5-bromo-4-chloro-3-□□phosphate-nitrotetrazolium chloride Dyeing solution (BCIP/NBT Solution). According to the structure of the high-performance mRNA labeling detection reagent group described in claim 1, wherein the gene chip is dehydrogenated with β-actin and glyceraldehyde 3-phosphate (Glyceraldehyde 3- Phosphate dehydrogenase, GADPH) as a housekeeping gene. According to the high-performance mRNA labeling detection reagent group structure described in claim 1, wherein the processing unit has an analysis software and an image capturing mode and a data transmission mode. The high performance mRNA labeling detection reagent set structure according to claim 1, wherein the processing unit is a single wafer or a programmable logic array. The high performance mRNA labeling detection reagent set structure according to claim 1, wherein the input unit is a plurality of buttons. The high performance mRNA labeling detection reagent set structure according to claim 1, wherein the output unit is a display screen. The high performance mRNA labeling detection reagent set according to the first aspect of the patent application, wherein the test system is blood, body fluid, cell culture or tissue cells.