TWM507430U - Biochemical reaction conduction electric connection - Google Patents

Description

Biochemical reaction conductive connector 【0001】

This creation relates to an electrical connector, especially a disposable biochemical reactive electrical connector.

【0002】

With the advancement of medicine and the increasing awareness of modern people's health care concepts, the market penetration of Point-of-Care testing (P0CT) has also increased. Such assays have the advantage of being fast, inexpensive, small, and requiring no professional handling, such as testing blood glucose, uric acid, cholesterol concentrations, and concentrations of heavy metals and pesticides in the sewage. In this field, the use of Metal Electrode's Electrochemical Sensor Strip has been an extremely sophisticated technology that has been used for decades in the detection of various fluids, such as electricity. Chemical detectors, biosensors, fluid biochemical sensor sensors, and detectors for a variety of home medical applications.

[0003]

The principle of an electrochemical sensor basically comprises: 1. a receiving container for accommodating a fluid to be tested; 2. a chemical reagent (Reagent) for analyzing an analyte with the fluid to be tested An electrochemical action is generated and an output signal of an electrical parameter is generated. The output value of the electrical parameter is related to the analyte of the fluid to be tested; 3. a plurality of test electrodes including a counter electrode (Working Electrode), a working electrode (Reference Electrode), and a detecting electrode ( Detecting Electrode), which can be used with different numbers of electrodes depending on the electrochemical requirements; 4. A measuring device for providing the necessary operating voltage, receiving the output signal and measuring a measured value to represent the analyte content. When the fluid to be tested is human blood and the analyte is blood sugar, then a glucose oxidase and other complexes can be used as a chemical reagent.

[0004]

The material of the above reference electrode (Reference Electrode) is most commonly used as a silver/silver chloride modified electrode (Modified Electrode). Because the electrochemical working potential of silver chloride is very stable, it is widely used as a reference electrode. The working electrode of Working Electrode is complicated and divided into two major directions. The first type is Electro-Transfer Mediator Modified Working Electrode, and the second type is metal catalytic electrode ( Metal-Catalyzed Electrode). In the first type, an electrochemical reagent is first immobilized on the electrode of the electron transfer medium, and the chemical reagent contains an enzyme (Enzyme such as glucose oxidase Glucose oxidase) and a redox reaction electron carrier (Redox Mediator, One of the Mediators widely used in blood glucose test strips is the sub-salt potassium ferricyanide).

[0005]

When a new chemical component (such as H202 is produced) is derivatized (or consumed) by chemical reaction with the analyte to be tested, and then released by the redox process of the electron carrier and the new chemical component (such as H202) The electron generates an electrical signal and outputs its electrical measurement parameter via the electrode. The purpose of such working electrodes is for conduction purposes only and does not have a chemically catalyzed function. However, the working electrode needs to be fabricated with an electrode material that does not chemically react with the fluid or chemical to be tested to avoid interference measurement accuracy. Commonly used electrode materials are mostly inert conductive materials, such as gold, platinum, palladium, ruthenium or other metals or carbon-containing electrodes, such as carbon film printing electrode Carbon Base Screen Printing Electrode, graphite rod Graphite Bar. Since carbon and inert metals do not chemically act at low temperatures, they do not cause chemical interference.

[0006]

In the case of precious metals, the cost is high, so the working electrodes of such Electron-Transfer Mediator Modified Working Electrode generally use carbon electrodes. As for the second type of metal-catalyzed electrode (Metal-Catalyzed Electrode), the selected working electrode material needs to be directly electrochemically reacted with a chemical reagent or an analyte or a derivative thereof, so that it can directly catalyze or have a single choice for the analyte. Sexual function. Therefore, the electrode itself can initiate a catalytic reaction, so that it is not necessary to add an electron transfer medium (Mediator) to the chemical reagent. Such an electrode material having direct catalysis is generally a metal electrode containing a metal component, but different metal electrode materials are required depending on the composition of the sample to be tested, and commonly used materials are copper, titanium, nickel, gold, and platinum. Metals such as palladium, rhodium, etc. (wherein the ruthenium electrode has an excellent direct catalytic effect on H202).

【0007】

There is an example of a disposable printed electrode on the market which uses an electrode printed by a paste-like conductive paste, the sensing effect of which is influenced by the composition of the paste-like conductive paste used. When a noble metal is used as the paste conductive paste, it can have better sensitivity and lower chemical interference, but the cost is very high. On the other hand, if the electrode is made of a conductive carbon film having a lower cost, the electrode can be effectively reduced in cost, but the electrode obtained is likely to cause an error in the measurement signal due to the high impedance. Furthermore, the use of electrodes made by printing or sputtering has a high cost and a high defect rate, and there is room for improvement in the test piece which is discarded once.

[0008]

One of the aims of the present invention is to provide a biochemical reaction conductive electrical connector which is easy to manufacture, stable in quality, high in yield, and conforms to mass production and low cost.

【0009】

In order to achieve the above purpose, the present invention provides a biochemical reaction conductive electrical connector for sensing the content of biochemical substances in a test object. The electrical connector includes an insulative body, a pair of electrodes, a pair of biochemical materials, and a cover sheet. The insulating body comprises a platform, a slot and an insertion end. The slot is opened at the front end of the platform, and the insertion end is connected to the rear end of the platform. The pair of electrodes are buried and formed in the insulating body. Each of the electrodes includes a reaction portion, a contact portion, and a conductive portion connecting the reaction portion and the both ends of the contact portion, wherein each contact portion is exposed on a surface of the insertion end. Each biochemical material is placed on each reaction section. The cover sheet covers the platform.

[0010]

The creation also has the following effects: the electrode is integrally formed by injecting and injecting (insert injection molding) into the insulating body, and has the advantages of easy manufacture, stable quality, high yield and high mass production, thereby reducing cost. The advantages. In addition, the integral projections are used to connect the platform upright, so that the user can easily hold the electrical connector so that the hand does not pollute or interfere with the accuracy of the measurement of the electrode, the biochemical material and the side object (fluid).

[0029]

100‧‧‧Electrical connector

[0030]

102‧‧‧Insulated body

[0031]

104‧‧‧Insert

[0032]

110‧‧‧ platform

[0033]

120‧‧‧ slot

[0034]

130‧‧‧Reaction zone

[0035]

140‧‧‧ storage tank

[0036]

150‧‧‧ ventilation channel

[0037]

160‧‧‧Bumps

[0038]

170‧‧‧ interval

[0039]

180‧‧‧Connected flat area

[0040]

190‧‧‧Arc Department

[0041]

200‧‧‧electrode

[0042]

210‧‧‧Reaction Department

[0043]

220‧‧‧Transmission Department

[0044]

230‧‧‧Contacts

[0045]

240‧‧‧Interference Department

[0046]

300‧‧‧Biochemical materials

[0047]

500‧‧‧ coverslips

[0048]

510‧‧‧ Covered Body

[0049]

520‧‧‧View area

[0050]

530‧‧‧Flap

[0051]

540‧‧‧ raised end

[0052]

600‧‧‧Testing instruments

[0053]

610‧‧‧ slots

[0054]

620‧‧‧Positioning holes

[0055]

630‧‧‧Connecting tape

[0011]

FIG. 1 is an exploded view showing the biochemical reaction conducting electrical connector of the present invention.

[0012]

FIG. 2 is an exploded view showing the assembled cover sheet after the creation electrode and the insulating body are integrally formed.

[0013]

3 is a cross-sectional view showing the biochemical reaction conductive electrical connector of the present invention.

[0014]

FIG. 4 is a schematic diagram showing the combination of the biochemical reaction conductive electrical connector of the present invention.

[0015]

FIG. 5 is a schematic view showing the connection of the biochemical reaction conducting electrical connector of the present invention to a detecting instrument.

[0016]

FIG. 6 is an exploded perspective view showing each electrode connecting strip of the present invention.

[0017]

This creation is a single-use biochemical reaction conductive electrical connector. Preferably, the biochemical material used in the present creation is a reaction enzyme, which is originally a liquid or a paste, and becomes a body after drying. The reaction enzyme component includes, but is not limited to, a biologically active substance (eg, an enzyme, an antibody, an antigen, etc.), an enzyme cofactor, a stabilizer (such as a high molecular polymer), a capillary penetration enhancer (such as a surfactant), a buffer solution, and the like. The high molecular polymer is used for immobilizing a biologically active substance such as PVP (Polyvinyl pyrrolidone) or PVA (Polyvinyl alcohol). The detailed description and technical content of the present invention are described below with reference to the drawings, but the drawings are only for reference and explanation, and are not intended to limit the creation.

[0018]

As shown in FIG. 1 and FIG. 2, the present invention provides a biochemical reaction conductive electrical connector for sensing the content of a biochemical substance in a test object (not shown). The electrical connector 100 includes an insulative housing 102, a pair of electrodes 200, a pair of biochemical materials 300, and a cover sheet 500. In other embodiments, the number of the electrodes 200 may be set to be more than one pair, and the biochemical materials 300 are correspondingly arranged according to the number of the electrodes 200. The insulative housing 102 includes a platform 110, a slot 120 and an insertion end 104. The slot 120 is open at the front end of the platform 110, and the insertion end 104 is connected to the rear end of the platform 110. The pair of electrodes 200 are buried and molded into the insulative housing 102. Each of the electrodes 200 includes a reaction portion 210, a contact portion 230, and a conductive portion 220 connecting the reaction portion 210 and the two ends of the contact portion 230. The contact portions 230 are exposed on the surface of the insertion end 104. Each of the biochemical materials 300 is provided on each of the reaction units 210. The cover sheet 500 covers the platform 110.

[0019]

The insulating body 102 is made of a general plastic injection material, and a thermoplastic material such as ABS, PS, PBT or glass fiber is used. The electrode 200 is continuously stamped and formed into a stainless steel sheet, and at the same time, the surface of the two reaction portions 210 and the contact portion 230 is sputtered with a zirconium alloy coating (not shown) or other suitable precious metal. At this time, the two electrodes 200 of the tape (as shown in FIG. 6) are connected and joined to the mold (not shown), and the integrated immersion molding is performed. In order to have a better engagement effect with the insulative housing 102 , each of the conductive portions 220 of each electrode 200 further has at least one interference portion 240 . The interference portion 240 protrudes from the side edge of the conductive portion 220 to create a better engagement relationship with the insulative housing 102. Therefore, the two electrodes 200 are integrally formed by immersing and insert molding into the insulating body 102, and have the advantages of easy manufacture, stable quality, high yield, and high mass production, thereby reducing cost.

[0020]

In the embodiment shown in FIGS. 1 and 2, the insulative housing 102 further includes a reaction zone 130 from which the reaction zone 130 is raised. The slot 120 is further disposed on the reaction zone 130. In the present embodiment, the reaction zone 130 preferably further includes two arc portions 190. Each of the circular arc portions 190 is disposed at a joint of the slit 120, the reaction zone 130, and the cover sheet 500. That is to say, if too much air is formed in the slot 120, the arc portion 190 disposed on both sides of the slot 120 will help reduce the resistance of the side object (not shown) and make the side object flow more. Smooth.

[0021]

Referring to FIG. 3 at the same time, the reaction zone 130 further includes two storage slots 140 and a ventilation channel 150. Each storage tank 140 is formed at the front end of the platform 110 and is disposed corresponding to the slot 120, wherein the venting channel 150 is connected to the slot 120. Each of the biochemical materials 300 is further disposed in each storage tank 140 and attached to the surface of each reaction portion 210 for biochemical reaction with the side object (not shown), and conducts current to each contact portion 230 through each reaction portion 210. . That is, when the side object (liquid) enters from the slot 120, the venting channel 150 communicating with the slot 120 allows gas to be easily discharged from the venting channel 150 without obstructing the side to be circulated into the storage tank 140. In addition, the side material and the biochemical material 300 are biochemically reacted and electron transfer occurs.

[0022]

As shown in FIG. 2 and FIG. 3 , each reaction part 210 is embedded and molded into each storage tank 140 , and each of the conductive parts 220 is embedded and molded into the platform 110 , and the height of each reaction part 210 is higher than each of the conductive parts 220 . The height of the setting. Further, each of the reaction portions 210 is vertically/directly connected to each of the conductive portions 220, so that each of the reaction portions 210 has a relatively good conduction function after the biochemical material 300 reacts with the side material (not shown).

[0023]

Referring to FIG. 4 together, the cover sheet 500 is made of soft plastic or other suitable material. The cover sheet 500 is attached to the reaction zone 130 and has a cover sheet body 510, an inspection area 520, a plurality of fins 530 and a raised end 540. The inspection area 520 is disposed in the middle of the cover sheet body 510, and is made of a transparent material to visually inspect the side object (not shown) inhalation and/or reaction state. Each of the fins 530 protrudes from both ends of the cover sheet body 510. In this embodiment, it is preferable to have four fins 530 protruding from the four side edges of the cover sheet body 510. The raised end 540 is disposed at the front edge of the cover sheet body 510 to facilitate alignment of the object to be side and to concentrate the side object into the slot 120.

[0024]

As shown in FIG. 1 to FIG. 4, the insulative housing 102 further includes a plurality of integrally formed injection-shaped bumps 160. Each of the bumps 160 is connected to the platform 110 in an upright manner, and a spacer 170 is further disposed between the bumps 160 on the same side. The flap 530 is engaged and positioned. In addition, in the embodiment shown in FIG. 4, the two sides of the reaction zone 130 further comprise two connection plane zones 180, and each connection plane zone 180 connects the platform 110 and the reaction zone 130 respectively, so that the reaction zone 130 and the platform 110 are A gentle slope is formed to facilitate the attachment of the cover sheet body 510, so that the object to be tested (not shown) does not overflow from the slot 120. Therefore, the plurality of bumps 160 are used to connect the platform 110 upright, so that the user can easily hold the electrical connector 100 without contaminating or interfering with the accuracy of the biochemical material 300 and the side object (fluid) measurement.

[0025]

As shown in FIG. 5, a schematic diagram showing the connection of the biochemical reaction conductive electrical connector to a detecting instrument is shown. The present electrical connector 100 is preferably used in conjunction with the testing instrument 600. The insertion end 104 of the electrical connector 100 is inserted into the slot 610 of the testing instrument 600, and the biochemical data related to the side body (not shown) is read and further analyzed by the detecting instrument 600.

[0026]

Further, when the detection is performed, the insertion end 104 having the contact portion 230 is inserted into the slot 610 of the detecting instrument 600, and the object to be tested (for example, blood, urine, etc.) is covered by the cover sheet 500 to narrow the reaction area 130. The slot 120 is open and is siphoned into the slot 120 of the reaction zone 130. The analyte enters the storage tank 140 to rapidly generate a biochemical reaction with the biochemical material 300 of the reaction portion 210, and is formed by the electrode 200 to form a pathway to the contact portion 230, wherein the electron transfer is generated by the biochemical reaction of the oxidized or reduced substance with the analyte. Used in conjunction with the detection instrument 600 to detect the concentration of the analyte or other information.

[0027]

As shown in FIG. 6 , the electrode 200 is formed by being conveniently embedded with the insulative housing 102 , and is generally connected by a strip 630 provided with at least one positioning hole 620 . As shown in FIG. 6, the end edge of each of the conductive portions 220 is further provided with a positioning hole 620 for positioning on the plastic mold during plastic injection molding. The positioning hole 620 on the connecting strip 630 is also used as a positioning hole for positioning on the plastic mold during plastic injection molding. After the buried incidence molding is completed, the connecting strip 630 can be separated from the respective electrodes 200 to complete the electrical connector 100 of the present invention.

[0028]

In summary, the embodiments disclosed herein are to be considered as illustrative of the present invention and are not intended to limit the present invention. The scope of this creation is defined by the scope of the appended patent application and covers its legal equivalents and is not limited to the foregoing description.

100‧‧‧Electrical connector

102‧‧‧Insulated body

104‧‧‧Insert

110‧‧‧ platform

120‧‧‧ slot

130‧‧‧Reaction zone

140‧‧‧ storage tank

150‧‧‧ ventilation channel

160‧‧‧Bumps

170‧‧‧ interval

190‧‧‧Arc Department

200‧‧‧electrode

210‧‧‧Reaction Department

220‧‧‧Transmission Department

230‧‧‧Contacts

240‧‧‧Interference Department

300‧‧‧Biochemical materials

500‧‧‧ coverslips

510‧‧‧ Covered Body

520‧‧‧View area

530‧‧‧Flap

540‧‧‧ raised end

Claims (12)

[Item 1] A biochemical reaction conducting electrical connector for sensing the content of a biochemical substance in a test object, the electrical connector comprising:
An insulative housing includes a platform, a slot and an insertion end, the slot is formed at a front end of the platform, and the insertion end is connected to a rear end of the platform;
a pair of electrodes embedded in the insulative housing, each of the electrodes includes a reaction portion, a contact portion, and a conductive portion connecting the reaction portion and the two ends of the contact portion, wherein each of the contact portions is exposed The insertion end surface;
a pair of biochemical materials, each of which is disposed on each of the reaction portions; and a cover sheet covering the platform. [Item 2] The biochemical reaction conducting electrical connector of claim 1, wherein the insulating body further comprises a reaction zone, the reaction zone is raised from the middle of the platform to form the slot, and the reaction zone is disposed in the slot. [Item 3] The biochemical reaction conducting electrical connector according to claim 2, wherein the reaction zone further comprises two storage tanks and a venting channel, each of the storage tanks being formed at a front end of the platform and disposed corresponding to the slot, the ventilation The channel is connected to the slot. [Item 4] The biochemical reaction conducting electrical connector according to claim 2, wherein each of the biochemical materials is further disposed in each of the storage tanks and attached to the surface of each of the reaction portions. [Item 5] The biochemical reaction conducting electrical connector according to claim 2, wherein each of the reaction portions is embedded and molded into each of the storage tanks, and each of the conductive portions is buried and formed in the platform, and the height of each of the reaction portions is set. Higher than the set height of each of the conducting portions. [Item 6] The biochemical reaction conductive electrical connector of claim 2, wherein the cover sheet is attached to the reaction zone, the cover sheet further has a cover sheet body, a view area and a plurality of fins, wherein the view area is disposed on the cover sheet In the middle of the cover sheet body, each of the fins protrudes from both sides of the cover sheet body. [Item 7] The biochemical reaction conducting electrical connector of claim 6, wherein the cover sheet body further comprises a raised end for facilitating alignment of the side object and for concentrating the side object into the slot. [Item 8] The biochemical reaction conducting electrical connector of claim 6, wherein the insulating body further comprises a plurality of bumps, each of the bumps being connected upright to the platform, and each of the bumps on the same side is further provided with a space for The flap is snap-fitted. [Item 9] The biochemical reaction conducting electrical connector according to claim 1, wherein each of the reaction portions further comprises a zirconium alloy plating film formed on a surface of the reaction portion, and each of the reaction portions is perpendicularly connected to each of the conductive portions. [Item 10] The biochemical reaction conducting electrical connector according to claim 1, wherein each of the conducting portions has at least one interference portion, and the at least one interference portion protrudes from a side edge of the conducting portion for engaging with the insulating body. [Item 11] The biochemical reaction conducting electrical connector according to claim 1, wherein each of the end portions of the conducting portion is further provided with a positioning hole. [Item 12] The biochemical reaction conducting electrical connector of claim 1, further comprising a connecting strip provided with a positioning hole, wherein the pair of electrodes are respectively connected by the connecting strip.