TWM408699U - Optical fiber module for detecting thermal convection polymerization chain reaction - Google Patents

Description

V. New Description: [New Technology Field] [0001] [0003] [0004] This creation is about thermal convection polymerase chain reaction devices, especially for detecting thermal convection polymerase chain reaction. Optical module. [Prior Art] Polymerase chain reaction (PCR) amplification of specific nucleic acid sequences is a well-established technology, which is an important technology in medicine and biotechnology. The reaction process has three main steps: "denaturation reaction" and "synthesis". The reaction and the "extension reaction", in which the three steps require different reaction temperatures. In today's commercial PCR equipment, the required reaction sample contains the model lion to be amplified, and the template, each; |^% acid-cut primer pair 'thermal stability DNA poly-triophosphate (dNTP). The PCR device circulates the reaction sample at three different temperatures by repeatedly heating and cooling the reaction sample, thereby amplifying a specific portion of the template]) nucleic acid sequence. ... The first step of PCR is denatured ash μ: it is to heat the reaction sample to a temperature of 'to make the double-stranded template DNA* salty. For a single-strand dna, the temperature of the typical denaturation reaction is between 90 and 95 ° C range. The second step of the PCR is a refining reaction, in which the reaction sample separated into single-stranded DNA is first cooled to a lower temperature, so that the primer is combined with the early strand DNA formed in the first step to form a composite of DNA and primer. The temperature of the typical refining reaction is selected according to the melting temperature (Tm) of the primer used, usually in the range of 35 to 65 ° C. Form No. A0101 Page 3 of 20 [0005] M408699 The third step of PCR is a polymerization (extension) reaction, which is to maintain a reaction sample of a complex forming a DNA and a primer at an appropriate temperature, and the primer is extended by the action of DNA polymerase to form a complementary strand with the template DNA. The new single-stranded DNA has a typical polymerization temperature of 72t. Therefore, for each cycle consisting of the above three steps, twice the template DNA can be replicated, and the PCR cycle including three steps of denaturation, refining reaction, and extension reaction is repeated about 20 to 40 times. A replica of millions of standard nucleic acid sequences can be produced. [0007] Please refer to "Figure 1", in the traditional commercially available PCR instrument (that is, thermal cycle instrument), in order to detect the concentration of the replica of the nuclear sequence copied by PCR, the reaction sample 7 will be dyed. Agent, μ allows the replica of the nucleic acid sequence to be stained by the dye' and further sets an optical system for debt measurement, the optical system comprises a light source 1, a multi-wavelength excitation filter 2 (Excitation Filter), an optical lens group 3. A filter wheel 4 and a CCD lens 5, the light generated by the light source 1 passes through the excitation light filter 2 to form a detection light source 6 of a specific segment wavelength (the figure is 5 different zones can be selected) The excitation light filter 2) of the segment wavelength is incident on the reaction sample 7 via the guiding of the optical lens group 3 to excite the fluorescent light 8, and the fluorescent light 8 is guided by the optical lens group 3 through the filter rotating disk 4 (Selecting the filter corresponding to the segment wavelength), and then entering the CCD lens 5, the CCD lens 5 detects the light intensity to know the concentration of the replica of the nucleic acid sequence. In order to simultaneously process a plurality of reaction samples 7, the optical lens group 3 must use a fresnel lens 3, a condensing lens 3B corresponding to a plurality of reaction samples 7, and a multi-element lens 3C (Multi-Element Lens) And so on to use the Fresnel lens 3A and the collecting lens 3B to detect the form number A0101 page 4 / 20 pages M408699 light source 6 is incident on a plurality of reaction samples 7, and the plurality of elements are redundantly separated by the multi-element lens The fluorescent light 8 of the reaction sample 7 is imaged on the CCD lens 5, respectively, to detect the light intensity. Therefore, conventional PCR instruments have difficulty in designing the optical path and require the use of expensive special lenses, which results in a relatively complicated optical system and high cost, which is difficult to be miniaturized and high in manufacturing cost, and it is difficult to meet the demand for cost reduction.

[0010] [New Content] Therefore, the main purpose of this creation is to expose a fiber optic module that avoids complexity.

II ...Μ This creation is a fiber mode for detecting thermal convection.

Can be used for thermal convection polymerase chain reaction anti-J light path design, while reducing the manufacturing of the base if. > [0011] a group for detecting a reaction sample doped with a dye, comprising a test tube holder, a light source, at least one reaction tube, at least one fiber transmission element, at least one wavelength selection element and a photosensitive element The test tube holder has at least one insertion space, the light source is disposed adjacent to the test tube holder, and the at least one reaction tube is inserted one-to-one on the at least one insertion space, and is filled with the dye-adding agent. a reaction sample, and the detection light source generated by the light source is incident on the at least one reaction tube, and the reaction sample mixed with the dye is excited to generate fluorescence. [0012] At least one end of the at least one fiber transmission component is separated by a condensing mirror Adjacent to the at least one insertion space, and receiving the fluorescent light, the other end of the at least one optical fiber transmission component is adjacent to the at least one wavelength selection component, and guides the fluorescent light through the at least one wavelength selection component. Form No. A0101 Page 5 of 20 [0013] At least one wavelength selection element receives the light intensity for detecting fluorescence. According to this, the creation of the reaction sample allows the fluorescent light to be guided through the optical fiber transmission component, and the wavelength selection component removes unnecessary wavelengths and is detected by the photosensitive component to know the light intensity of the fluorescent light. Therefore, it is not necessary to consider the optical path design, and it is not necessary to use an expensive special lens, and can be easily miniaturized and reduced in manufacturing cost, thereby meeting the need for cost reduction. [Embodiment] [0014] In order to make the members aware of the characteristics, purposes and effects of the creation, a better understanding and recognition of the present invention will be given, and the description will be followed by the following description: [0015] Please refer to the figure. 2" and "Fig. 3", as a fiber optic module for detecting a thermal convection polymerase chain reaction, for detecting a dye-doped reaction sample 10' comprising a test tube holder 20, a light source 3〇, at least one reaction tube 40, at least one fiber transmission element 5, at least one wavelength selection element 60 and a photosensitive element 70, wherein the test tube holder 2 has at least one insertion space 21, the light source 30 is in close proximity The test tube holder 20 is disposed. The light source 30 can be disposed on the bottom surface of the test tube holder 20, and the light source 3 generates a full-wavelength detection light source (white light), and the light source 30 can be a light-emitting diode, a laser, or a halogen. Any one of a lamp, a xenon lamp, and a xenon lamp. [0016] the at least one reaction tube 40 is inserted one-to-one on the at least one insertion space 21, and is configured to load the reaction sample 10 doped with the dye. The dye is based on the reaction sample 1 The item is selected, and if multiple indicators are to be detected, a plurality of dyes that do not interfere with each other may be added, and the detection source generated by the light source form nickname A0101 page 6/20 pages M408699 30 is incident on the at least one reaction. In the case of the test tube 40, the reaction sample 10 doped with the dye is excited to generate fluorescence.

[0017] Further, one end of the at least one optical fiber transmission component 50 is disposed adjacent to the at least one insertion space 21 via a condensing mirror 52, and the condensing mirror 52 is any one of a Fresnel lens and a convex lens, such as the at least one insertion space. Each of the sides of the 21 can be provided with a cavity 22, each of which is embedded with a fiber connector 80A. The fiber connector 80A has two clamping blocks 81A. The two clamping blocks 81A sandwich the optical fiber transmission component 50, which allows the One end of the at least one optical fiber transmission element 50 is adjacent to the side of the at least one insertion space 21 to receive the fluorescent light. The mirror side of the at least one insertion space 21 may further be provided with a mirror 23, and the light collecting direction of the reflecting mirror 23 faces the sand. a light-emitting member 50 is charged with I-' < *% 51; the reflected fluorescent light enters the at least one optical fiber transmission and is not transmitted * '. ·, f ' [0018] the at least one optical fiber transmission component 50 The other end is adjacent to the at least one wavelength selecting component 60 and directs the fluorescent light to pass through the at least one wavelength selecting component 60, and the photosensitive component is immediately adjacent to the zero, and the at least plant length selecting component 60

And receiving the light intensity for detecting the fluorescent light, so that the photosensitive element 70 can be a complementary metal oxide semiconductor (CMOS), a photosensitive coupling element (CCD), a photodiode, a phototransistor, a photomultiplier tube (PMT), a photoresistor, Any of a contact photosensitive device (CIS), a spectrophotometer, and a spectrometer. And each of the optical fiber transmission elements 50 may have a plurality of optical fibers 51, the number of the at least one wavelength selection component 60 corresponding to the plurality of optical fibers 51, and the plurality of optical fibers 51-to-one corresponding to the at least one wavelength selective component 60. According to the plurality of optical fibers 51, the plurality of fluorescent lights can be guided to pass the plurality of fluorescent lights through the wavelength selecting elements 60 of different wavelengths to separate the fluorescent light excited by the different dyeing agents, and the wavelength selecting component 60 can be filtered. Mirror, three form number A0101, page 7 / total 20 pages [0020] M408699 [0022] [0024] [0024] A horn mirror, a band pass beam splitter, etc., as long as it can filter out unwanted wavelengths, Or separate the required wavelengths. The present invention can be further provided with a component frame 90. The at least one wavelength selection component 60 is mounted on the component frame 90. The component frame 90 is provided with a card window 91. The card window 91 is provided with a fiber connector 80B. The joint 80B has two clamp blocks 81B that sandwich the optical fiber transmission member 50. Referring to FIG. 4 again, the two clips 81A of the present invention may further be provided with an inner flange 82 for pressing the fiber optic transmission component 50, and the two clips 81A are provided with a card that is mutually engaged. The slot 811 and a hook 812 are used to fasten the fiber optic transmission component 50 by snapping to prevent the fiber optic transmission component 50 from falling off. Please refer to FIG. 5 again, wherein the at least one optical fiber transmission component 50 can be made of an acryl having an absorption wavelength of 550 nm or less, and the at least one wavelength selection component 60 is coupled to the at least one optical fiber transmission component 50. In other words, it is no longer necessary to use the wavelength selective element 60 to eliminate unnecessary wavelengths, thereby saving costs. Please refer to FIG. 6 again, wherein the photosensitive element 70 and the component holder 90 are disposed on a sliding rail 75, and at least one of the optical fiber transmission components 50 can be moved differently. As described above, after the reaction sample 10 generates fluorescence, the creation of the optical fiber transmission member 50 allows the fluorescent light to pass through the wavelength selection element 60 to exclude unnecessary wavelengths and is received by the photosensitive element 70. Detection, you can know the light intensity of the fluorescent light, so this design does not need to consider the light path design, without the need for expensive special lenses, and can be easily miniaturized and form number A0101 page 8 / total 20 pages M408699 Manufacturing costs to meet the need to reduce costs. [0025] The above description is only for the preferred embodiment of the present invention, and is not intended to limit the scope of implementation of the present invention, that is, the equivalent changes and modifications according to the content of the patent application scope of the present invention should be Technical Field of Creation [Simple Description of Drawings] [0026] FIG. 1 is a schematic view showing the structure of a conventional optical module. [0027] FIG. 2 is a structural diagram of the fiber optic module of the present invention.

This is another angle structure diagram of the fiber optic module. This is the creation of the fiber clip, which is another fiber module, 4 &4; [0028] Figure 3

4 [0030] FIG. 5 [0031] FIG. 6 is a diagram showing the movement structure of the photosensitive member and the component holder. [Main component symbol description] ' 1 .

[0033] Conventional [0033] 1 : Light source [0034] 2 : Excitation light filter [0035] 3: Optical lens group [0036] 3A: Fresnel lens [0037] 3B: Condenser lens [0038] 3C: Multi-element lens [0039] 4: Filter turntable form number A0101

Page 9 of 20 M408699 [0040] 5 : CCD lens [0041] 6 : Detection light source [0042] 7 : Reaction sample [0043] 8 : Fluorescence [0044] This creation [0045] 10 : Reaction sample [ 0046] 20 : Test tube holder [0047] 21 : Interposed space [0048] 22 : Cuvette [0049] 23 : Mirror [0050] 30 : Light source [0051] 40 : Reaction tube [0052] 50 : Fiber optic transmission element [ 0053] 51 : Optical fiber [0054] 52 : Condenser [0055] 60 : Wavelength selection element [0056] 70 : Photosensitive element [0057] 75 : Slide rail [0058] 80A, 80B: Fiber connector form No. A0101 Page 10 / Total 20 pages M40.8699 [0059] 81A, 81B: clamp [0060] 811: card slot [0061] 812: hook [0062] 82: inner flange [0063] 90: component frame [0064] 91: card window

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Form No. A0101 Page 11 of 20

Claims (1)

M408699 VI. Patent Application Range: 1. A fiber optic module for detecting a thermal convection polymerase chain reaction for detecting a reaction sample doped with a dye, comprising: a test tube holder having at least one insertion a light source disposed adjacent to the test tube holder; at least one reaction tube, the at least one reaction tube being inserted one-to-one on the at least one insertion space, and for loading the reaction sample doped with the dye, And detecting light source generated by the light source is incident on the at least one reaction tube, and exciting the reaction sample mixed with the dye to generate fluorescence; at least one optical fiber transmission element, one end of the at least one optical fiber transmission element is adjacent to the condensing mirror Having at least one interposition space and receiving fluorescence; at least one wavelength selection element, the other end of the at least one fiber optic transmission element is adjacent to the at least one wavelength selection element, and directs fluorescence through the at least one wavelength selection element; a photosensitive element adjacent to the at least one wavelength selective element and receiving light intensity for detecting fluorescence. 2. The fiber optic module for detecting a thermal convection polymerase chain reaction according to claim 1, wherein the light source is disposed on a bottom surface of the test tube holder. 3. The fiber optic module for detecting a thermal convection polymerase chain reaction according to claim 1, wherein one end of the at least one fiber optic transmission element is adjacent to a side of the at least one interposing space. 4. The fiber optic module for detecting a thermal convection polymerase chain reaction according to claim 3, wherein a side of each of the at least one insertion space is provided with a cavity, and each of the slots is embedded with a fiber connector. The fiber optic connector has two clamps that sandwich the fiber optic transmission component. 100205073 Form No. A0101 Page 12 of 20 1002016411-0 M40.8699 5. A fiber optic module for detecting a thermal convection polymerase chain reaction as described in claim 4, wherein the two clamps are forced An optical fiber module for detecting a thermal convection polymerase chain reaction according to the fourth aspect of the invention, wherein the two clamping blocks are provided with a card slot and a hook for fastening each other. . 7. The fiber optic module for detecting a thermal convection polymerase chain reaction according to claim 1, wherein each of the fiber optic transmission elements has a plurality of optical fibers, and the number of the at least one wavelength selection component corresponds to a plurality of optical fibers, and The plurality of optical fibers are adjacent to the at least one wavelength selection element in a one-to-one correspondence. 8. The fiber optic module for detecting a thermal convection polymerase chain reaction according to claim 7 of the patent application, wherein one of the components is disposed on the component frame. p The fiber optic module of the heat-to-sweat 4 mulberry synthase chain reaction according to item 8 of the patent application scope, wherein the component frame is provided with a card window, the card window is provided with a fiber connector, and the fiber connector has two clamping blocks. The two clips sandwich the fiber optic transmission component. 10. As described in the scope of patent application No. 8, the lack of sidestream polymerase chain reaction ~ ·» The optical fiber module, wherein the photosensitive element carrier is disposed on a sliding rail and moves corresponding to at least one optical fiber transmission component. 11. The fiber optic module for detecting a thermal convection polymerase chain reaction according to claim 1, wherein the light source is any one of a light emitting diode, a laser, a halogen lamp, a gas lamp, and a xenon lamp. 12. The fiber optic module for detecting a thermal convection polymerase chain reaction according to claim 1, wherein the condensing mirror is any one of a Fresnel lens and a convex lens. 100205073 wavelength selection element Mu, shouting the fiber optic module of the M408699, as described in the scope of claim 1, the detection of the thermal convection polymerase chain reaction form number A0101 page 13 / 20 pages 1002016411-0 13 M408699, wherein the at least one fiber optic transmission component is The absorption is made by an acryl having a wavelength of 550 nm or less, and the at least one wavelength selective element is incorporated into the at least one optical fiber transmission element. 14. The fiber optic module for detecting a thermal convection polymerase chain reaction according to claim 1, wherein the photosensitive element is a complementary metal oxide semiconductor (CMOS), a photosensitive coupling element (CCD), and a photodiode. , Photoelectric crystal, photomultiplier tube (PMT), photoresistor, contact photoreceptor (CIS), spectrophotometer and spectrometer. The fiber optic module for detecting a thermal convection polymerase chain reaction according to claim 1, wherein a circumferential side of the at least one interposing space is provided with a mirror, and a condensing direction of the mirror faces the At least one fiber optic transmission component. The fiber optic module for detecting a thermal convection polymerase chain reaction according to claim 1, wherein the wavelength selective element is any one selected from the group consisting of a filter, a triangular mirror and a band pass spectroscope. 100205073 Form No. A0101 Page 14 of 20 1002016411-0