M407755 五、新型說明： 【新型所屬之技術領域】 本創作係關於-種包埋結構，特別是關於1 防陰道感染之乳酸菌及其代謝產物的多層包埋結構j、預 【先前技術】M407755 V. New description: [New technical field] This is a multi-layered structure of the lactic acid bacteria and its metabolites, which are related to the anaesthetic structure, and the pre-existing technology.
在母體内的胎兒係處於無菌狀態，而女性 生後的數小時，錄道_可培養岭乡細旨，^出 陰道内會形成-群正常的陰道_，其中，以卜 道的優勢菌種。 囷馮陰 存在於陰道的乳_群係具有分解陰道_原的功 用’且該乳酸菌群所分泌的代謝產物（如乳酸、過氧化 等）能夠幫助轉—穩定酸鹼值（約PH3.5〜4·5)，用以抑 制或殺死致病g。—般而言，只要陰勒維持—正常菌群， 陰道就不易受到外來病原菌之感染。 舉例來4，陰道感染可能來自細菌性感染、徽菌感染 或滴蟲感染。細菌性感染包括由陰道伽氏桿菌（㈤仙 )、大腸桿菌（汾咖咖細⑺/小綠膿桿菌 (seudomonas aeruginosa )、金黃色葡萄球菌 ⑽财飾娜）及鏈球菌屬（如脚⑽讀）等 ★斤引起，而极菌感染通常為白色念珠球菌（ 遷cww)所引起；滴蟲感染則係由性行為或公共浴池、游 冰池等環境間接傳染而得。 近年來有研究發現，陰道菌群係可來自於腸胃道黏膜 、皿生菌群’更詳言之’經由口服方式獲得—益生菌並持 M407755 續服用一段時間後，能夠間接使陰道獲得該益生菌，並且 於陰道中形成一陰道菌群，而避免陰道相關等疾病發生 (Reid G，Bruce AW·，Postgrad Med J. 2003, 79:428-432)。 目前用乳酸菌幫助治療或預防陰道感染的相關菌種包含有 嗜酸乳桿菌（ZacMflczY/ws )、比菲德氏菌 (Bifidobacterium bifidum)、短乳酸桿菌（Lactobacillus 保加利亞乳酸桿菌（z山泣等， 該群乳酸菌係利用其所分泌之代謝產物（如乳酸、過氧化 氫、細菌素等物質）、抗菌蛋白（antimicr〇biai peptide )、 菌體内的蛋白質或是競爭在陰道細胞上的附著能力，使陰 道内之酸驗值維持於正常範圍内，進而抑制陰道感染之病 原菌（如黴菌及細菌）的生長以維持陰道内之正常菌相。 目前市面上有許多不同型式的乳酸菌產品，大致上可 分為四種：（1)發酵乳品及飲料類、（2)奶粉類、（3)錠 片、膠囊類及（4)餅乾、糖果、口嚼片類，其中又以發酵 乳品及錠劑膠囊型式之產品應用最為廣泛，特別係以膠囊 型式之乳酸菌產品的效果較佳。 藉由口服方式獲得該乳酸g產品之益生菌時，由於人 體腸胃道中的胃酸及膽鹽會分解進人腸胃道中的食物，因 此’-般乳酸菌產品在進人腸胃道中，該乳_即被胃酸 及膽鹽破壞而死亡，且該乳酸騎產生之代謝產物也會失 去活性。 I知具有乳酸g之包埋結構係以—單層的膠囊膜形 成-谷置空間’且該容置空㈣以容置該乳酸菌之粉末或 顆粒，以該單㈣_對該乳酸菌進行包埋，該習知包埋 4 結構用以提供保龍乳_通過腸胃道，使該乳酸菌產品 進入腸胃道時不被腸胃道分解而失去活性。 然而，以單層膠囊膜構成之習知包埋結構無法防 酸菌及其代謝產物與氧氣接觸而使乳酸菌之 物氧化，而使該乳酸菌產品之活性降低，因此， 結構具有錢敎且耐久保存該乳_及其代謝產物之缺 點，尚有待改良之空間。 【新型内容】 本創作之主要目的係提供-種具有預防陰道感染之 乳酸菌保縣的包埋結構，践健具有_陰道感染之 礼酸菌及其代職物，使服帛者㈣該具有 威染 之乳酸菌及其代謝產物後，能於陰道維持—正常=相二進 而有效抑制陰道病原菌之生長。 本創作之次-目的係提供—種具有預防陰道感染之 乳酸菌保護層的包埋結構’能夠防止該乳酸菌及其代謝產 物與氧氣產生氧化作用，穩定且耐久地财乳酸菌及其代 謝產物。 為達到前述創作目的，本創作所運用之技術内容包含 有： y種具有預防陰道感染之乳酸菌保護層的包埋結 構’係包含核心；-乳酸菌LA25保護層係包覆於該 核心之外周面，以及-殼層係包覆於該乳酸菌LA25保護 層之外周面。 其中’該核心係由-醣類組合物所構成之球體，該核 心係包含由乳糖、嚴糖、菊糖、果寡糖、木寡糖、海藻糖、 半乳糖及寡糖所組成之群。 ,/、中該乳酸菌LA25保護層係由一乳酸菌經醋酵而 %·之酸酵液’該醱酵液係經由—乳化劑乳化形成之殼層。 該撥酵液包含該乳酸菌及其代謝產物，該乳酸g係嗜酸乳 舍干菌Uactobacillus acidophUusLA25);該乳化傲係包含由 大丑油、玉米油、葵花油、葡萄籽油、撖欖油、芝麻油及 花生油所組成之群。 其中，該殼層係包含一耐膽鹽層及一抗胃酸層，該耐 膽鹽層係包覆於該乳酸菌LA25保護層之外周面，該抗胃 酸層係包覆於該耐膽鹽層之外周面。該耐膽鹽層係由一高 分子膠群組所構成之殼層，該耐膽鹽層係包含由果膠、海 藻膠、鹿角菜膠及甲基纖維素鈉所組成之群；該抗胃酸層 係由一乳蛋白群組所構成之殼層，抗胃酸層係包含由動物 膠、脫脂奶粉、酪蛋白及乳清蛋白所組成之群。 【實施方式】 為讓本創作之上述及其他目的、特徵及優點能更明顯 易懂’下文特舉本創作之較佳實施例，並配合所附圖式， 作詳細說明如下： 請參照第1圖所示，係本創作之具有預防陰道感染菌 之乳酸菌保護層的包埋結構示意圖，該包埋結構係包含一 核心11、一乳酸菌LA25保護層12及一殼層13，該乳酸 菌LA25保護層12係包覆於該核心η之外周面，且該殼 層13係包覆於該乳酸菌LA25保護層12之外周面，用以 M407755 . 抵，腸胃道中的胃酸及膽鹽破壞，並阻隔外界環境對該乳 . 酸鲕LA25保護層12產生的氧化作用。 該核心11係由-麵組合物所構叙球體用以供該 ，菌LA25保護層12吸附該核心11，且提供該乳酸菌； ㈤之營養纽。本實施例之如Η係包含但稀定由乳 糖、蔗糖、菊糖、果寡糖、木寡糖、海藻糖、半乳糖及寡 糖所組成之群，或者其他適用於本創作之材料。 • 該乳酸菌LA25保護層12係包覆於該核心u之外周 • * ’該乳酸菌LA25保護層12係由一乳酸菌經酸酵而得^ 醱酵液，該醱酵液係經由一乳化劑乳化形成之殼層，該醱 酵液包含該乳酸_及其代謝產物（如乳酸、多聽等）。 本實施例之該乳酸菌係嗜酸乳桿菌（ ac油冲//⑽LA25，寄存編號為BCRC 910494)，該乳酸菌 之代謝產物係包含蛋白質、多醣體、脂肪酸、乳酸、乳酸 . 菌素（Bacteriocin)等之複合物，該代謝產物係具有抑^ ' 陰道病原菌生長之作用，以維持陰道内之正常菌相，達到 預防陰道感染之功效。 « 該乳化劑係包含但不限定由大豆油、玉米油、葵花 油、葡萄籽油、橄欖油、芝麻油及花生油所组成之群，戍 其他適用於本創作之乳化劑。該乳化劑係用以乳化該乳酸 菌而形成油包水之油滴，使該乳酸菌能夠穩定保存於油液 . 中，使該乳酸菌不受氧氣之氧化作用而失去活性，並且達 到穩定且耐久地保存該乳酸菌及其代謝產物之活性，藉此 形成一具有穩定且耐久保存功效之乳酸菌LA25保護層 12。 、° 曰 —7 — M407755 此外’本創作之乳酸菌LA25保護層12中另可添加有 · 磷脂質、維生素c '維生素E、維生素B群、胺基酸等營 ’ 養成份，可更加輔助該乳酸菌LA25保護層12穩定保存該 礼酸菌之效果’且該維生素C及e除了能提供該乳酸菌所 需之營養外，另可作為一抗氧化劑使用，更可提高該乳酸 菌LA25保護層12耐久保存之功效。 S玄殼層13係包含一耐膽鹽層131及一抗胃酸層132， 該财膽鹽層131係包覆於該乳酸菌LA25保護層12之外周 面，該抗胃酸層132係包覆於該耐膽鹽層131之外周面。φ 該耐膽鹽層131係由一高分子膠群組所構成之殼層，The fetus in the mother is in a sterile state, and the female is counted for several hours after birth. The _ can be cultivated in the township, and the vagina will form a normal vaginal vaginal vaginal vaginal vaginal genus.囷Feng Yin exists in the vagina milk _ group has the function of decomposing vaginal _ original 'and the metabolites (such as lactic acid, peroxidation, etc.) secreted by the lactic acid bacteria group can help turn - stabilize the pH value (about PH3.5~ 4·5), used to inhibit or kill the diseased g. In general, as long as the sputum is maintained - the normal flora, the vagina is not susceptible to infection by foreign pathogens. For example, 4, vaginal infections may come from bacterial infections, infections of the genus or trichomoniasis. Bacterial infections include vaginal vaginal bacterium ((5) sinensis), Escherichia coli (汾 咖 咖 (7) / udo mon se (seudomonas aeruginosa), Staphylococcus aureus (10), and Streptococcus (such as the foot (10) read ), etc. caused by the pound, and the extreme bacterial infection is usually caused by Candida albicans (migrating cww); trichomoniasis infection is caused by sexual behavior or indirect infection of public baths, ice pools and other environments. In recent years, studies have found that the vaginal flora can be derived from the gastrointestinal mucosa, the bacteria-producing group 'more specifically' obtained by oral means - probiotics and holding M407755 for a period of time, can indirectly make the probiotic get the probiotic Bacteria, and form a vaginal flora in the vagina, avoiding diseases such as vaginal involvement (Reid G, Bruce AW, Postgrad Med J. 2003, 79: 428-432). Currently, lactic acid bacteria are used to help treat or prevent vaginal infections, including Lactobacillus acidophilus (ZacMflczY/ws), Bifidobacterium bifidum, and Lactobacillus (Lactobacillus lactis). Group lactic acid bacteria use the secreted products (such as lactic acid, hydrogen peroxide, bacteriocin, etc.), antibacterial proteins (antimicr〇biai peptide), proteins in the bacteria or the ability to compete on vaginal cells. The acid value in the vagina is maintained within the normal range, thereby inhibiting the growth of pathogenic bacteria (such as mold and bacteria) of the vaginal infection to maintain the normal bacterial phase in the vagina. There are many different types of lactic acid bacteria products on the market, which are roughly separable. There are four types: (1) fermented dairy products and beverages, (2) milk powders, (3) tablets, capsules, and (4) biscuits, candy, and chewable tablets, which are also fermented in dairy and lozenge capsules. The products are most widely used, especially in the case of capsule type lactic acid bacteria products. When obtaining the probiotics of the lactic acid g product by oral means Since the stomach acid and bile salts in the human gastrointestinal tract break down into the food in the human gastrointestinal tract, the lactic acid bacteria product enters the gastrointestinal tract, and the milk is destroyed by gastric acid and bile salts, and the metabolism of the lactic acid ride occurs. The product also loses its activity. I know that the embedding structure with lactic acid g is formed by a single layer of capsule film-valley space and the space is accommodated (4) to accommodate the powder or granules of the lactic acid bacteria to the single (four) _ Embedding the lactic acid bacteria, the conventional embedding structure is used to provide the colostrum milk _ through the gastrointestinal tract, so that the lactic acid bacteria product does not be decomposed by the gastrointestinal tract and loses activity when entering the gastrointestinal tract. However, it is composed of a single layer capsule film. The conventionally embedded structure is incapable of preventing acid bacteria and its metabolites from coming into contact with oxygen to oxidize the substances of the lactic acid bacteria, thereby reducing the activity of the lactic acid bacteria product, and therefore, the structure has money and durable storage of the milk and its metabolites. Disadvantages, there is still room for improvement. [New content] The main purpose of this creation is to provide an embedded structure of lactic acid bacteria Baoxian County with vaginal infection prevention, and it has _ vaginal infection The acid-staining bacteria and its substitutes enable the person to serve (4) the lactic acid bacteria and their metabolites, which can be maintained in the vagina - normal = phase two and thus effectively inhibit the growth of vaginal pathogens. Providing an embedding structure having a protective layer of lactic acid bacteria for preventing vaginal infections can prevent oxidation of the lactic acid bacteria and its metabolites with oxygen, and stabilize and durable lactic acid bacteria and their metabolites. To achieve the aforementioned creative purpose, the present invention The technical contents of the application include: y: an embedding structure having a lactic acid bacteria protective layer for preventing vaginal infection' contains a core; - a lactic acid bacteria LA25 protective layer is coated on the outer peripheral surface of the core, and a - shell layer is coated thereon The outer surface of the lactic acid bacteria LA25 protective layer. Wherein the core is a sphere composed of a saccharide composition comprising a group consisting of lactose, Yan sugar, inulin, fructooligosaccharide, xylooligosaccharide, trehalose, galactose and oligosaccharide. /,, the lactic acid bacteria LA25 protective layer is a lactic acid bacteria by vinegar and the acid lysate of the lysate is emulsified by an emulsifier to form a shell layer. The broth comprises the lactic acid bacteria and the metabolite thereof, and the lactic acid g is Uactobacillus acidophUusLA25); the emulsified arsenic comprises ugly oil, corn oil, sunflower oil, grape seed oil, eucalyptus oil, A group of sesame oil and peanut oil. Wherein, the shell layer comprises a gallbladder salt layer and a first anti-gastric acid layer coated on the outer surface of the protective layer of the lactic acid bacteria LA25, and the anti-gastric acid layer is coated on the bile-salt layer The outer perimeter. The bile-tolerant salt layer is a shell layer composed of a group of polymer gums comprising a group consisting of pectin, seaweed gum, carrageenan and sodium methylcellulose; the anti-gastric acid The layer consists of a shell composed of a group of milk proteins comprising a group consisting of animal glue, skim milk powder, casein and whey protein. [Embodiment] The above and other objects, features and advantages of the present invention will become more apparent. The following detailed description of the preferred embodiments of the present invention, together with the accompanying drawings, will be described in detail as follows: The figure shows a schematic diagram of the embedded structure of the lactic acid bacteria protective layer of the present invention for preventing vaginal infection, the embedded structure comprising a core 11, a lactic acid bacteria LA25 protective layer 12 and a shell layer 13, the lactic acid bacteria LA25 protective layer The 12-layer is coated on the outer peripheral surface of the core η, and the shell layer 13 is coated on the outer peripheral surface of the protective layer 12 of the lactic acid bacteria LA25 for M407755. The gastric acid and bile salts in the gastrointestinal tract are destroyed, and the external environment is blocked. Oxidation of the milk. Acid 鲕 LA25 protective layer 12. The core 11 is composed of a spheroid for constituting the sphere, and the bacterium LA25 protective layer 12 adsorbs the core 11 and provides the lactic acid bacterium; The lanthanide of this embodiment comprises, but is sparsely composed of, a group consisting of lactose, sucrose, inulin, fructooligosaccharides, xylooligosaccharides, trehalose, galactose and oligosaccharides, or other materials suitable for use in the present invention. • The lactic acid bacteria LA25 protective layer 12 is coated on the outer periphery of the core u. * 'The lactic acid bacteria LA25 protective layer 12 is obtained by acid fermentation of a lactic acid bacterium, and the lysate is emulsified by an emulsifier. The shell layer contains the lactic acid and its metabolites (such as lactic acid, polyphonic, etc.). In the present embodiment, the lactic acid bacteria Lactobacillus acidophilus (ac oil / / (10) LA25, accession number BCRC 910494), the metabolite of the lactic acid bacteria contains protein, polysaccharide, fatty acid, lactic acid, lactic acid, Bacteriocin, etc. The complex, the metabolite has the function of inhibiting the growth of vaginal pathogens to maintain the normal bacterial phase in the vagina and to prevent vaginal infection. « This emulsifier contains, but is not limited to, a group consisting of soybean oil, corn oil, sunflower oil, grape seed oil, olive oil, sesame oil and peanut oil, and other emulsifiers suitable for this creation. The emulsifier is used to emulsify the lactic acid bacteria to form a water-in-oil oil droplet, so that the lactic acid bacteria can be stably stored in the oil, so that the lactic acid bacteria are not deactivated by the oxidation of oxygen, and are stably and durablely preserved. The activity of the lactic acid bacterium and its metabolites thereby forms a protective layer 12 of lactic acid bacteria LA25 having stable and durable preservation effects. , ° 曰—7 — M407755 In addition, 'the lactic acid bacteria LA25 protective layer 12 of this creation can be added with phospholipid, vitamin c 'vitamin E, vitamin B group, amino acid and other camping ingredients, which can further assist the lactic acid bacteria. The LA25 protective layer 12 stably stores the effect of the bacterium, and the vitamins C and e can be used as an antioxidant in addition to the nutrients required for the lactic acid bacteria, and the lactic acid bacteria LA25 protective layer 12 can be further protected. efficacy. The S-shell layer 13 includes a gallstone-resistant layer 131 and an anti-gastric acid layer 132. The gallstone layer 131 is coated on the outer peripheral surface of the lactic acid bacteria LA25 protective layer 12, and the anti-gastric acid layer 132 is coated on the outer layer. The outer surface of the gallstone-resistant salt layer 131. φ The bile salt-resistant layer 131 is a shell layer composed of a group of polymer glues.
用以保邊該乳酸菌不受膽鹽之破壞。本實施例之耐膽鹽層 13係包覆於該乳酸菌LA25保護層12外層，該耐膽鹽層 131係包含但不限定由果膠、海藻膠、鹿角菜膠及曱基纖 維素鈉所組成之群’或其他適用於本創作之高分子膠。該 w分子膠使膽鹽不會接觸到該乳酸菌LA25保護層12，保 遵該礼酸菌不會目接糊雜，而使該乳_之細胞膜受 到膽鹽破壞。 I 该抗胃酸層132係由一乳蛋白群組所構成之殼層，用 以保濩该乳酸菌不受胃酸之破壞。本實施例之抗胃酸層 132係包含但不限定由動物膠、脫脂奶粉、酪蛋白及乳清 蛋白所組成之群，或其他適用於本創作之材料。該抗胃酸 層132使本創作之包埋結構能延長胃酸分解之效率，使該 礼酸菌能夠提高存活率並達到生物體之腸胃道中，以建立 陰道之益生菌菌相，並達到預防陰道感染之效果。 藉此，本創作具有預防陰道感染之乳酸菌LA25保護 8 M407755 層的包埋結構之殼層n將該乳酸紅Ah保護層 可避免該乳酸菌LA25係護層12受到腸胃道之胃酸是趟 之破壞，使服用者能夠攝取到有效量之乳酸菌，進而^ 陰道之盈生_相，且該乳酸菌及其代謝產物不會氣 化作用而*去活性，能崎刺久保存之效果。 本創作之具有預贈道錢之級_ la25保護 包埋結構’係用以保護該乳酸菌及其代謝產物之乳‘菌Used to preserve the lactic acid bacteria from the destruction of bile salts. The bile salt-resistant layer 13 of the present embodiment is coated on the outer layer of the lactic acid bacteria LA25 protective layer 12, and the bile-tolerant salt layer 131 is composed of, but not limited to, pectin, seaweed gum, carrageenan and sodium decyl cellulose. Group ' or other polymer glue suitable for this creation. The w-molecule gel prevents the bile salt from coming into contact with the protective layer 12 of the lactic acid bacteria LA25, and the cell membrane of the milk is not damaged by the bile salt. I The anti-gastric acid layer 132 is a shell layer composed of a group of milk proteins for protecting the lactic acid bacteria from gastric acid. The anti-gastric acid layer 132 of the present embodiment includes, but is not limited to, a group consisting of animal glue, skim milk powder, casein and whey protein, or other materials suitable for the present invention. The anti-gastric acid layer 132 enables the embedding structure of the present invention to prolong the efficiency of decomposition of gastric acid, so that the acid-producing bacteria can improve the survival rate and reach the gastrointestinal tract of the organism, thereby establishing a probiotic phase of the vagina and preventing vaginal infection. The effect. Therefore, the lactic acid bacteria LA25 for preventing vaginal infection protects the shell layer of the 8 M407755 layer of the embedding structure. The lactic acid red Ah protective layer can prevent the lactic acid bacteria LA25-based protective layer 12 from being damaged by the stomach acid of the gastrointestinal tract. The user can take an effective amount of lactic acid bacteria, and then the vaginal _ phase, and the lactic acid bacteria and their metabolites will not be vaporized and *reactivated, and can be used for long-term preservation. This creation has a pre-granted grade _ la25 protection embedding structure' is used to protect the lactic acid bacteria and its metabolites
層，使服用者服用該具有預防陰道感染之乳酸菌及其 產物後’能維持陰道之益生顏相，具有有效預 原菌生長之功效。 、爲 本創作之具有預防陰道感染之乳酸菌LA25保護層的 包埋結構’係具有—避免乳酸菌及其代謝產物接觸到氣氣 之乳酸菌層，使該乳_及其代謝產物不受氧氣之氧化作 用而失去雜’具有達刚|定且)^久地保存該乳酸菌及波 代謝產物之功效。 yThe layer allows the user to take the lactic acid bacteria and products thereof for preventing vaginal infections, and maintains the probiotic phase of the vagina, and has the effect of effectively pre-producing bacteria growth. The embedding structure of the lactic acid bacteria LA25 protective layer for the prevention of vaginal infections has a lactic acid bacteria layer which prevents the lactic acid bacteria and its metabolites from coming into contact with the air gas, so that the milk _ and its metabolites are not oxidized by oxygen. And the effect of the lactic acid bacteria and wave metabolites is preserved for a long time. y
雖然本創作已利用上述較佳實施例揭示，然其並非用 以=疋本創作，任何熟習此技藝者在不脫離本創作之精神 和範圍之内，相對上述實施例進行各種更動與修改仍屬本 創作所保護之技絲，，因此賴作之紐範圍當視後附 之申請專利範圍所界定者為準。 【圖式簡單說明】 第1圖：具有預防陰道感染之乳酸菌保護層的包埋結構 示意圖。 —9 — M407755 【主要元件符號說明】 〔本創作〕 11 核心 12 乳酸菌LA25保護層 13 殼層 131耐膽鹽層 132抗胃酸層Although the present invention has been disclosed by the above-described preferred embodiments, it is not intended to be used in the present invention. Any modifications and modifications to the above embodiments may be made by those skilled in the art without departing from the spirit and scope of the present invention. The technical wire protected by this creation, therefore, the scope of the application is subject to the definition of the patent application scope attached. [Simple description of the figure] Fig. 1: Schematic diagram of the embedding structure of the protective layer of lactic acid bacteria for preventing vaginal infection. —9 — M407755 [Description of main component symbols] [This creation] 11 Core 12 Lactobacillus LA25 protective layer 13 Shell layer 131 Bile salt resistant layer 132 Anti-gastric acid layer