M332520 八、新型說明： 【新型所屬之技術領域】 本創作係-種辅助微粒子分離之微流道構造，係在一 主流道中之至少-側設有複數個齒狀單元，藉由施予電場 以在主流道内產生非均句電場，造成不同微粒子移動產生 隨者電場改變交替出現之渦旋而有效分離微粒子。 【先前技術】 鲁 ㉒速生醫檢測技術對於人類疾病之檢測甚為重要，越 快速的得知檢測結果，便可為病患爭取更多的治療時間， 因此，利用微機電製程技術所製作之生物晶片便可提供此 一需求。 然而，隨著微型化之後，微機電製程技術所製作之生 物晶片，其元件中許多的行為便與大型系統產生極大的差 異，其中最值得注意的即是微管道内流體的特性。由於元 件尺度變小，因此流體於微管道中流動時的雷諾數 Φ ( Reyn〇lds number)非常小，其數值遠低於可以造成紊流 ，(turbulent flow)的2500，所以流體在未施加任何外力 時’於微管道中僅能以層流（laminar f i〇w)方式流動。 此一特性在微流體晶片中扮演著相當重要的角色，諸如： 樣本傳輸、聚焦以及樣本流道切換的作動，皆須仰賴層流 此一穩定的流場特性來達成。但其對於微流體晶片領域之 發展亦帶來了一需克服之難題，由於在從事任何生物反應 時’皆需在微管道内有效地操控微粒或細胞，包括對於細 胞或微粒的攫取或分離，以提升微流體晶片進行反應分析 M332520 2功此’以簡化生化分析檢測所需的時間與空間的需 二=流體係：層流的特性來流動，因此流體於微管道 机w ’僅能藉由擴散作用進行分離，所以往往需 =的微管道或較長的擴散時間，以達成不同種微粒子的^ =然而’此-作法卻減低了微型化所帶來輕薄短小及快 離欢測的優點’因此如何在微管道中進行快速微粒子分 離，便成為一重要的研究課題。 【新型内容】 本創作係提供一種改良習知缺點之輔助微粒子分離之 微流道構造，藉线道中設置之齒狀單元配合表面;= 4 (zeta potential)分布之改變來操控主流道内之流體。 本創作包括： 一主流道，具有一輸入端與一輸出端； 一正電極，位於主流道之輸入端； 一負電極，位於主流道之輸出端； 其中主流道内側之至少一側係設有複數個齒狀單元， 該齒狀單元係由管壁朝主流道軸線形成之漸 縮角度係為一銳角。 八浙 β藉由施予外加電場時，使齒狀單元之漸縮表面產生一 場茂漏（field leakage)之法線方向電場分量，形成一非 均勻電場，藉使主流道巾之微粒子被極化，由於不同介電 係數之微粒子所誘發的極化均不相同，在非均句電場作用 下，.不同微粒子移動產生隨著電場改變交替出現之渦璇 (circulation)進而有效分離微粒子。 ；M332520 上述輔助微粒子分離之微流道構造，其中齒狀單元之 漸縮角度為30度。 上述主μ道之材料係為電介體物質之矽、聚酯或丙烯 酸樹酯其中任一種或其組合。 …上述齒狀單疋之漸縮部位之材料係非均勻分布，齒狀 單元之漸縮4位之材料係在漸縮之端部形成較大壁之昱 _質區。 八 •本創作具有下列之優點： 〃 1·本創作係利用齒狀單元形成非均勻電場造成不同介 電係數之微粒子移動，產生隨著電場改變交替出現之渦 ^集中在不同強弱的電場區域中，分離微粒子之速度較 擴散快。 2·本創作可廣泛應用於微流道晶片，提高其不同之微 粒子間之分離效率，可強化微流道晶片之功能性與分析之 精確性。 _ 3·利用電場的調控，將不再須要另外設計其它的微粒 -收集官這，大大縮短進行分離時所需的空間。 【實施方式】 請參閱第一圖，本創作之第一實施例包括： 一主流道（1)，具有一輸入端（11)與一輸出端（12); 一正電極（圖中未示出），位於主流道（1)之輸入端 (11); 一負電極（圖_未示出），位於主流道（1)之輸出端 (12); M332520 • 上述主〃IL道（1)内側之至少一侧係設有複數個齒狀單 兀（2) ’本實&例中係在主流道⑴兩侧^有對稱之齒狀 單元⑴，該齒狀單元（2)係由管壁朝主流道⑴轴線 形成之漸縮部位’其漸縮角度（㊀）係為一銳角，本實施 .例中該銳角為30度。該主流道⑴的寬度為糊^，其中 主仙·道（1)之材料係為電介體物質之矽、聚酯或丙烯酸樹 ，醋其中任一種或其組合，且齒狀單元（2)之材料分布係為 籲非均勻分布’在齒狀單元（2)之漸縮部位之材料係在漸縮 之端部形成較大壁厚之異質區（3)，藉以提高齒狀單元（2) 之表面異質性，藉此強化電場之非均勻度。 實施時，接上電源並外加電場，於主流道（1)入口處 注入微粒子歸，本實_巾之餘子錢係包括：去離 子水直彳工3β m之粒子，之後將主流道（1 )接上電壓為v 之電源’於主流道（1)外加電場，初始時為較小的電場一， 本實施例中電場-之值為，Cffl，待電解液分別充滿整個 鲁管道後，將電場提高至電場二，本實施例中電場二之值為 380V/cm。當微粒子通過齒狀單元（2)，產生迴流現象，調 控外加電場強度，使微粒子集中在不同強弱的電場區域中 進而分離微粒子。 本創作之第二實施例如第二圖所示，係在主流道（4) 兩側設置相對交錯之不對稱之齒狀單元（5)，該齒狀單元 (5)係由管壁朝主流道軸線形成之漸縮部位，其漸縮角度 (Θ!)係為一銳角，本實施例中該銳角為如度，該主流道 (4)的寬度為400 "ra’其中主流道（4)之材料係為；二M332520 VIII. New description: [New technical field] The creative system is a micro-channel structure that assists in the separation of fine particles. It is provided with a plurality of tooth-shaped units on at least one side of a main channel, by applying an electric field. A non-sequential electric field is generated in the main channel, causing different microparticle movements to generate vortices that alternate with the electric field change and effectively separate the microparticles. [Prior Art] Lu 22-speed biomedical detection technology is very important for the detection of human diseases. The faster the learning results are, the more treatment time can be obtained for patients. Therefore, the organisms made by MEMS technology are used. The chip can provide this demand. However, with the miniaturization, the micro-electromechanical process technology produced by the micro-electromechanical process technology, many of its components are very different from the large-scale system, the most notable of which is the characteristics of the fluid in the micro-pipe. As the dimension of the component becomes smaller, the Reynolds number Φ (Reyn〇lds number) when the fluid flows through the microchannel is very small, and its value is much lower than 2500 which can cause turbulent flow, so the fluid is not applied at all. In the case of external force, it can only flow in a laminar fi〇w manner. This feature plays a very important role in microfluidic wafers, such as: sample transfer, focusing, and sample flow switching, all relying on laminar flow, a stable flow field characteristic. However, its development in the field of microfluidic wafers has also brought about a problem that needs to be overcome, because in any biological reaction, it is necessary to effectively manipulate microparticles or cells in microchannels, including the extraction or separation of cells or particles. In order to improve the reaction analysis of the microfluidic wafer, the M332520 2 works to simplify the time and space required for biochemical analysis and to detect the flow and space requirements of the laminar flow. Therefore, the fluid can only be used by the micropipeline machine. Diffusion is used for separation, so it is often necessary to use micro-pipes or long diffusion times to achieve different kinds of micro-particles. However, this method reduces the advantages of miniaturization, lightness, shortness and fast separation. Therefore, how to perform rapid particle separation in micro-pipes has become an important research topic. [New content] This creation provides a micro-fluidic structure that assists in the separation of fine particles by conventional disadvantages. The toothed cells in the line are matched with the surface; the change in the zeta potential is used to manipulate the fluid in the main channel. The creation comprises: a main channel having an input end and an output end; a positive electrode located at the input end of the main channel; a negative electrode located at the output end of the main channel; wherein at least one side of the inner side of the main channel is provided A plurality of toothed units are formed at an acute angle by a tapered angle formed by the wall of the tube toward the axis of the main flow path. By applying an applied electric field, Bazha β causes the tapered surface of the toothed unit to generate a normal direction electric field component of the field leakage, forming a non-uniform electric field, so that the microparticles of the mainstream towel are polarized. Since the polarizations induced by the micro-particles with different dielectric coefficients are different, under the action of the non-uniform electric field, the movement of different micro-particles produces a vortex that alternates with the change of the electric field to effectively separate the micro-particles. ; M332520 The microfluidic structure of the above-mentioned auxiliary microparticle separation, wherein the toothed unit has a tapered angle of 30 degrees. The material of the above main channel is any one or a combination of a dielectric substance, a polyester or an acrylic resin. The material of the tapered portion of the toothed single crucible is non-uniformly distributed, and the tapered material of the toothed unit is formed at the end of the tapered portion to form a larger wall. VIII. This creation has the following advantages: 〃 1. This creation uses the toothed unit to form a non-uniform electric field to cause the movement of particles with different dielectric coefficients, and the vortices that alternate with the change of the electric field are concentrated in the electric field regions with different strengths and weaknesses. The speed of separating the particles is faster than the diffusion. 2. This creation can be widely applied to micro-channel wafers to improve the separation efficiency between different micro-particles, and to enhance the functionality and analysis accuracy of micro-channel wafers. _ 3. With the regulation of the electric field, it is no longer necessary to design another particle-collecting officer, which greatly shortens the space required for separation. [Embodiment] Referring to the first figure, the first embodiment of the present invention includes: a main channel (1) having an input terminal (11) and an output terminal (12); a positive electrode (not shown in the drawing) ), located at the input (11) of the main channel (1); a negative electrode (Fig. _ not shown) at the output (12) of the main channel (1); M332520 • inside the main 〃IL (1) At least one side is provided with a plurality of toothed single cymbals (2). In this example, a symmetrical toothed unit (1) is affixed on both sides of the main flow path (1), and the toothed unit (2) is composed of a pipe wall The tapered portion (a) formed toward the axis of the main channel (1) has an acute angle (a) which is an acute angle. In the present embodiment, the acute angle is 30 degrees. The width of the main channel (1) is paste, wherein the material of the main channel (1) is a dielectric substance, a polyester or an acrylic tree, a vinegar of any one or a combination thereof, and a tooth unit (2) The material distribution is a non-uniform distribution of the material at the tapered portion of the toothed unit (2) to form a heterogeneous zone (3) having a larger wall thickness at the tapered end, thereby improving the toothed unit (2) Surface heterogeneity, thereby enhancing the non-uniformity of the electric field. When it is implemented, connect the power supply and apply an electric field, and inject the fine particles into the entrance of the main channel (1). The remaining money of the actual _ towel includes: deionized water directly to the 3β m particles, and then the mainstream channel (1) Connect the power supply with voltage v to the main channel (1) to apply an electric field. Initially, it is a small electric field. In this embodiment, the value of the electric field is Cffl. After the electrolyte is filled with the entire Lu pipeline, The electric field is increased to the electric field 2. In this embodiment, the value of the electric field two is 380 V/cm. When the microparticles pass through the toothed unit (2), a reflow phenomenon is generated, and the applied electric field strength is adjusted, so that the microparticles are concentrated in different electric field regions to separate the microparticles. A second embodiment of the present invention, as shown in the second figure, is provided with a relatively staggered asymmetrical toothed unit (5) on both sides of the main flow path (4), the toothed unit (5) being directed from the tube wall toward the main channel The tapered portion formed by the axis has an angle of inclination (Θ!) which is an acute angle. In this embodiment, the acute angle is as follows, and the width of the main channel (4) is 400 "ra' where the main channel (4) Material is;