M296369 八、新型說明： 【新型所屬之技術領域】 本創作相關於一種具有排除干擾功能的電化學生物 感測試紙，尤指-種具有對照組而可消除雜訊干擾的電化 學生物感測試紙。 【先前技術】 由於科技的進步，從前許多需要經由大型檢測儀器檢 測的試驗，現在可改由掌上型的機台即可進行檢測，不但 更為方便使用，且適合小型診所或居家使用，@目前市面 上有許多用過一次後直接丟棄且用於居家自行操作之生 物感測試紙’適合非專業人員及居家護财便使用，並且 配&μ的生物感測器裝置’即可測量出正確 值。 一習用之電化學生物感測試紙具有一基板、一導電膜、 包ίίΐί-上隔板’在該基板上形成導電膜’該導電膜 導雷腺:、2 "互不相接觸的陽極部分和陰極部分，在該 導雷膜陽，部覆蓋一層電絕緣層而裸露出部分導電膜，該 ==分及陰極部分裸露出之部分一端形成工作 /考電極’而另一端則可與生物感測器裝置連接， 及參考電極上覆蓋-反應區，該反應區視 入後「待刺2作’並於反應區上覆蓋上隔板。當待測物吸 ,,"即會與反應區物質產生反應並產生電化學變 i頭與^電§極與參考電極傳導至導電膜另一端之陰極 妾碩，猎由與生物感測器裝置連接並接收訊 M296369 唬，=過计异而將訊號轉換成待測物濃度顯示。 了而由於樣品中有一些物質會造成非特異性的反 應，^者有些物質或環境亦會影響電化學的改變，因此造 成測量結果比實際值偏高或偏低，使得測量結果不準確， 故有待進一步的改進。 【新型内容】 為了改善上述習用缺失，本創作提供一種具有排除干 擾力月b的電化予生物感測試紙，其係具有複數個反應區， 5中：個反應區含有生物活性物質（如酵素）可與待測物 貝進仃特異性反應，而另一個反應區除不含生物活性物質 外其餘物負與如一反應區相同，藉此，即可將不含生物活 1物質的反應區所產生的反應作為背景值而扣除干擾物 質所造成的結果偏低或偏高，進而達到更為準確的測量結 果。 為了達到上述目的，本創作之技術手段在於提供一種 •具有排除干擾功能的電化學生物感測試紙，其係包括 一基板， 至少二條工作電極，其係設於基板上且互不相接觸； 至少-❹考電極’其係設於基板上且與該工作電極 互不相接觸；及 至少二個反應區，其係用以進行反應並與工作電極和 參考電極相接觸，且該至少二個反應區係分別相間隔不接 觸而分別接觸獨立的工作電極及一參考電極，其中一個反 5M296369 VIII. New description: [New technical field] This creation is related to an electrochemical biosensor test paper with interference-eliminating function, especially an electrochemical biosensor test paper with control group to eliminate noise interference. . [Prior Art] Due to advances in technology, many previous tests that need to be tested by large-scale inspection instruments can now be tested by handheld machines, which are not only more convenient to use, but also suitable for small clinics or home use, @现There are many bio-sensing test papers that are directly discarded after one use and used for home operation. It is suitable for non-professionals and home care, and can be measured correctly with &μ biosensor device. value. A conventional electrochemical biosensing test paper has a substrate, a conductive film, and an upper separator to form a conductive film on the substrate. The conductive film guides the gland: 2 " an anode portion that does not contact each other And the cathode portion, in the positive portion of the lightning guiding film, is covered with an electric insulating layer to expose a portion of the conductive film, and the portion of the portion where the == part and the exposed portion of the cathode portion form a working/test electrode' and the other end is compatible with the biological feeling The detector device is connected, and the cover electrode is covered with a reaction zone, and the reaction zone is treated as "to be stabbed 2" and covered with a partition plate on the reaction zone. When the analyte is sucked, " The substance reacts and produces an electrochemical change, and the cathode and the reference electrode are conducted to the cathode of the other end of the conductive film. The hunting is connected with the biosensor device and receives the signal M296369 唬, The signal is converted to the concentration of the analyte to be detected. However, some substances in the sample may cause non-specific reactions, and some substances or environments may also affect the electrochemical changes, thus causing the measurement results to be higher or biased than the actual values. Low, making the test The result is inaccurate, so it needs further improvement. [New content] In order to improve the above-mentioned abuse, the present invention provides an electrochemical pre-sensitivity test paper with a disturbing force month b, which has a plurality of reaction zones, 5: one The reaction zone contains a biologically active substance (such as an enzyme) to specifically react with the test substance, while the other reaction zone is the same as the reaction zone except that it does not contain the biologically active substance, thereby The reaction produced by the reaction zone containing the biologically active substance 1 is used as a background value and the result of deducting the interfering substance is lower or higher, thereby achieving a more accurate measurement result. In order to achieve the above object, the technical means of the present invention is to provide An electrochemical biosensor test paper having a function of eliminating interference, comprising a substrate, at least two working electrodes, which are disposed on the substrate and are not in contact with each other; at least the reference electrode is disposed on the substrate and Not in contact with the working electrode; and at least two reaction zones for reacting and contacting the working electrode and the reference electrode And the at least two reaction zones are spaced lines without contact respectively in contact with the working electrode and a separate reference electrode, a counter 5 wherein
M296369 應區含有與一待測物質產生特異性反應的生物活性物質 及其他進行反應所需物質，而另一反應區則不含該特異性 反應的生物活性物質而含有其他進行反應所需物質。 本創作所述之具有排除干擾功能的電化學生物感測 試紙可進-步包括—巾隔板，其係設於該基板及工作電極 和茶考電極的部份並具有一凹槽，該凹槽係裸露出部分的 工作電極和參考電極，且該反應㈣設於該巾隔板的凹槽 _本創作所述之具有排除干擾功能的電化學生物感測 -式、’、氏可進步包括一上隔板，其係設於中隔板上而與基板 形成毛細現象空間，較佳地，該上隔板具有—缺口，該缺 口相應於該中隔板的凹槽處，更佳地，該缺Π呈半圓形或 半橢圓形。另外’該中隔板的凹槽係設於該中隔板的一側 或一端，而該凹槽係呈一门字型或呈一匸字型。 ^ 不剧作所述之具有排除干擾功能的電化學 測試紙中’該複數個反應區係接觸同-參考電極， 而分別接觸獨立的工作電極。 ^ ,.1 i 11 ^ ^ ^# ^^1 ^ ^ ^ ^ 々生物活性物質可為酵素。 試紙::由士„有排除干擾功能的電化學生物感測 社里、由计含生物活性物質（如酵素）的反應區之 、°【實:二;’】即可將雜訊過濾，增加測量結果的正：性。 M296369 凊茶考第一至三J1I辦-++ 示意圖，本創作之：有排二搭係本創作第-種實施例之 紙包括一基板（1 〇、）、i擾功能的電化學生物感測試 至少-個參考電極（24Γ:,工作電極(20、22)、 1)、-中隔板⑴及個反應區（4〇、4 η、或一々/ 〇 )及一上隔板（5 0 )，該基板（i 為一巨形片體’較佳地具有電絕緣特性。 :少二個工作電極（20、22)係設於基板（1 0^2 2 2)而，1作電極(2〇)及第二工作電極(2 上㈣^；二—個參考電極（2 4 )係設於基板（1 〇 ) 極(2〇、22)互不相接觸，該第-工 ^ 一 ）、第一工作電極（2 2)及參考電極（2 )t一端係延伸朝向基板（1 0)的一端。 該至少二個反應區（4 〇 ,,隻店鹿 {4 ° 4丄）於本實施例中係包 括-第-反應區（40)及一第二反 :反應區（4Q)係含有酵素、酵素辅助因子、安㈣= 及緩衝溶液等，其係設於基板（10)工 ==及參考電極（24)上，而該第二反應區 铷哲 與第一反應區（4〇)除了酵素外的所有 :質’：免產生特異性反應，該第-反應區 二：=區（41)互不相接觸’而該第一反應區（40; 極（20)及參考電極（24)接觸，該 ==(41)係與第二工作電極（22)與該參考 (2 4 )相接觸，該第一反應區（4 〇)與該第二反 M296369 應£ ( 4 1 )係共用同一參考電極（2 4 )，該第一反應 區（4 0 )及第二反應區（4 1 )係設於該基板（1 〇 ) 的側邊。 該中隔板（3 0 )係覆蓋於基板（1 〇 )、工作電極 (20、22)及參考電極（24)之部分，並設有一凹 槽（3 1 )’該凹槽（3 1 )係設於相應於該第一反應區 (40)及第二反應區（41)處，即可藉由該中隔板（3 0)暴露出第一反應區（4〇)及第二反應區（4 1)， 1較佳地，該凹槽（3 1 )係設於該中隔板（3 〇 )的一側 且呈一门字型，而該第一反應區（4 0 )及第二反應區（4 1 )分別位於该门字型呈縱向的兩道，藉由該中隔板（3 0)的區隔而將第一反應區（4〇)及第二反應區（41) 分開，避免造成相互的干擾。 該上隔板（5 0 )係設於該第一反應區（4 〇 )、第 二反應區（4 1 )、工作電極（2 〇 )及參考電極（3 〇) 丨上，藉由上隔板（5 Q )與基板（1 〇 )間於相應反應區 (4 0、4 1 )處及中隔板（3 0 )凹槽（3 1 )的設置 而幵^成毛細現象空間而令待測物可經由毛細現象吸入反 應區（4 0、4 1 )間，較佳地，該上隔板（5 〇 )於才目 應於反應區（4 0、4 1 )處朝内側凹設有一缺口（ 5 1 )， 4缺口（ 5 1 )设於該上隔板（5 〇 )的側邊，更佳地， 該缺口（ 5 1 )呈半圓形或半橢圓形，藉此，即可加速 測物進入反應區"0、4 D，或者，於該 〇)相應於反應區（4 0、4 1 )處設有孔洞，待測物即 8 M296369 可直接滴入反應區（40、41)而與反應區（4〇、4 1 )物質反應，另外亦可不需於中隔板（3 〇 )上覆蓋上 隔板（5 0 )。 本創作使用時，首先將待測物經由毛細現象吸入或滴 入的反應區（4 0、4 1 )後，較佳地，若為吸入的方式， 則待測物由上隔板（5 〇)缺口（5工）的作用加速地同 時進入第一反應區（4.0 )及第二反應區（4工）中，而 令待測物分別與第一反應區（4 〇)和第二反應區（4工） 物i進行反應，该待測物接觸第一反應區（4 〇 )後會使 得乾燥試劑溶解並進行反應，進而產生電化學的改變Y唑 由與第一反應區（4 0 )接觸之第一工作電極（2 〇 )及 參考電極（2 4 )傳導，*由生物感卿裝置連接並接收 另外待測物接觸第二反應區（4 i )後使得乾燥試 劑洛解並進行反應，進而產生電化學的改變，由於該第二 反應區（4 1 )不含酵素與酵素輔助因子，因此所^生: 反應係為干擾物所產生的反應，而並非所要測量物質的特 :性反應’因此將第一反應區（4 〇 )所測得的結果扣除 第二反應區（41)所測量的結果，即 量物質的濃度。 ㈣ /f參考第四至六圖所示，其係本創作之第二種實施例 之不思圖，该試紙包括一基板（1 2 ) 極（26、27)、至少一個參考電 個 個反應區（42、43)、一中隔板（ 二― (52)。該工作電極（26、27、/ 。反 电位1 J b 2 7)係設於該基板（1 9 M296369 2 j上且互不相接觸，較佳地，該工作電極（2 6、2 7 ) 為第一工作電極（26)及第二工作電極（27)，而該 參考電極（2 8 )係設於該基板（1 0 ) I而與該工作雷 、6 ϋ ζ 7 )互不相接觸。該中隔板（3 2 )係霜筌 了於$基板（1〇)、工作電極（26、27)及參考電 極（2 ^ )的—部份，且該中隔板（32)具有一凹槽（3 3 ) ’ $凹槽（3 3 )係設於該中隔板（3 2 )的-端， 較佳地、，該凹槽（33)係為一 tr字型。該反應區（42、 j為第一反應區（42)及第二反應區（43)，該 $反應區（42)及第二反應區（43)係設於該凹槽 八3 3 )呈c字型的橫道上，其中，該第一反應區（4 2 ; 二有酵素、酵素輔助因子、安定劑(如高分子聚合物）及 等，而該第二反應區（4 3 )係含有與第-反應 .)除了酵素外的所有物質，另，該第一反應區（4 相接η一工作電極（2 6)及該參考電極（2 8) ?. 而5亥第二反應區（4 3 )與該第二工作電極（2 ” 參考電極（2 8 )相接觸，即該第一反應區（4 右二、弟二反應區（4 3 )#用-參考電極（2 8 )而具 工作電極（26、27)。該上隔板（52)係 a又於该中隔板（q 缺口 （ 3 2 )上方，且該上隔板（5 2 )具有一 2)騎缺口（53)相應於該第-反應區" 該試紙二:(43)處，即該缺口（53)位於 嵩使用時，由古+ & 1 "式、、、氏相應於缺口（ 5 3 )的一端吸入血 M296369 液，則血液經由毛細現象進入中隔板（3 2 )的凹槽（3 3)處，並與第一反應區（42)和第二反應區（43) 的物質進行反應，由於該第一反應區（4 2 )含有酵素， 因此可與血液中欲測量的物質進行特異性的反應，然而血 液中存在著干擾物質會與第一反應區（4 2 )的其他物質 叙生假性反應，因此造成雜訊干擾，故而由第二反應區（4 3)所產生的電化學改變即為該些假性反應所造成的結 果，將第一反應區（4 2 )所產生的結果扣除第二反應區 (4 3 )所產生的結果即可測量出準確的數值。 綜上所述，由於具有至少二個反應區，而其中一個反 應^含有與待測物質進行反應的所有物質，故而不論是欲 測量的物質或是會造成假性反應的物質通通都會作用，而 另:反應區則含有除酵素外的所有物質，故若為非測量的 物i會產生的反應都會顯現，因此經由扣除不含酵素的反 應區的結果，即可得到準確的結果，藉此，本創作之具有 排除干擾功能的電化學生物感測試紙可將非特異性^應 造成的雜訊扣除，進而達到快速檢測以及測量準確的目 的0 ▲雖然前述實例說明本創作之具體事實，但應瞭解任何 可能的修正及改變均不悖離以下主張之申請專利範圍的 精神及範®壽。 【圖式簡單說明】 第—圖係本創作具有排除干擾功能的電化學生物感測 試紙第一種實施例之上視示意圖。 一圖將上隔板移開後之上視示意The M296369 area contains biologically active substances that specifically react with a substance to be tested and other substances required for the reaction, while the other reaction zone does not contain the biologically active substance of the specific reaction and contains other substances required for the reaction. The electrochemical biosensor test paper having the function of eliminating interference described in the present invention may further include a towel spacer disposed on the substrate and the working electrode and the tea test electrode and having a groove, the concave The working system and the reference electrode are exposed in the groove, and the reaction (4) is provided in the groove of the towel separator. The electrochemical biosensing method with the function of eliminating interference described in the present invention can be improved. An upper partition plate is disposed on the middle partition plate to form a capillary phenomenon space with the substrate. Preferably, the upper partition plate has a notch corresponding to the groove of the middle partition plate, and more preferably, The defect is semi-circular or semi-elliptical. Further, the groove of the middle partition is disposed at one side or one end of the middle partition, and the groove is in the shape of a gate or a U-shape. ^ In the electrochemical test paper having the interference-eliminating function described above, the plurality of reaction zones are in contact with the same-reference electrode and are respectively contacted with the independent working electrodes. ^ ,.1 i 11 ^ ^ ^# ^^1 ^ ^ ^ ^ 々 Bioactive substances can be enzymes. Test strip:: by the „the electrochemical biosensing agency with the function of eliminating interference, the reaction zone containing bioactive substances (such as enzymes), °[real: two; '] can filter the noise, increase Positive results of the measurement results. M296369 第一茶考第一第一三J1I办-++ Schematic, the creation of this: There are two rows of the first embodiment of the paper, including a substrate (1 〇,), i The electrochemical biosensor of the disturbance function tests at least one reference electrode (24Γ: working electrode (20, 22), 1), - middle separator (1) and a reaction zone (4〇, 4 η, or a 々/〇) and An upper spacer (50), the substrate (i is a giant shaped sheet' preferably has electrical insulating properties.: two less working electrodes (20, 22) are attached to the substrate (1 0^2 2 2 ), 1 for the electrode (2〇) and the second working electrode (2 (4)^; 2—the reference electrode (2 4 ) is disposed on the substrate (1 〇) pole (2〇, 22) are not in contact with each other, The first working electrode (1), the first working electrode (2 2) and the reference electrode (2) t are extended to one end of the substrate (10). The at least two reaction zones (4 〇,, only shop deer { 4 ° 4丄) in this real In the example, the first reaction zone (40) and the second reverse reaction zone (4Q) contain an enzyme, an enzyme cofactor, an anion (four) = and a buffer solution, which are provided on the substrate (10) == And the reference electrode (24), and the second reaction zone and the first reaction zone (4〇) except for the enzyme: all: ': to avoid a specific reaction, the first reaction zone two: = zone ( 41) not contacting each other' and the first reaction zone (40; the pole (20) and the reference electrode (24) are in contact, the == (41) is associated with the second working electrode (22) and the reference (2 4) In contact with each other, the first reaction zone (4 〇) and the second counter M296369 should share the same reference electrode (2 4 ), the first reaction zone (40) and the second reaction zone (4) 1) is disposed on a side of the substrate (1 〇). The middle spacer (30) covers a portion of the substrate (1 〇), the working electrode (20, 22), and the reference electrode (24), and is provided There is a groove (3 1 ) 'the groove (3 1 ) is disposed at a position corresponding to the first reaction zone (40) and the second reaction zone (41), that is, by the middle partition (3 0) Exposing the first reaction zone (4〇) and the second reaction (4 1), 1 preferably, the groove (3 1 ) is disposed on one side of the middle partition (3 〇) and has a gate shape, and the first reaction zone (40) and the The two reaction zones (4 1 ) are respectively located in two longitudinal directions of the gate shape, and the first reaction zone (4〇) and the second reaction zone (41) are separated by the middle partition (30). Separate to avoid mutual interference. The upper partition (50) is disposed in the first reaction zone (4 〇), the second reaction zone (4 1 ), the working electrode (2 〇), and the reference electrode (3 〇 ) 丨, by the arrangement of the upper partition (5 Q ) and the substrate (1 〇) at the corresponding reaction zone (40, 4 1 ) and the middle partition (30) groove (3 1 ) ^ into the capillary phenomenon space so that the object to be tested can be sucked into the reaction zone (40, 4 1 ) via capillary phenomenon, preferably, the upper partition (5 〇) is in the reaction zone (40, 4) 1) a notch (5 1 ) is formed in the inner side, and 4 notches ( 5 1 ) are disposed on the side of the upper partition (5 〇). More preferably, the notch ( 5 1 ) is semicircular or semi-circular. Elliptical, by which you can accelerate the measurement into the reaction zone �,4 D, or, In this case, a hole is formed corresponding to the reaction zone (40, 4 1 ), and the analyte, 8 M296369, can be directly dropped into the reaction zone (40, 41) to react with the reaction zone (4〇, 4 1 ). Alternatively, it is not necessary to cover the upper partition (5 )) with the upper partition (5 0 ). In the present use, the test object is first inhaled or dropped into the reaction zone (40, 4 1 ) via capillary action, preferably, if it is inhaled, the test object is separated by the upper partition (5 〇 The effect of the notch (5 work) is accelerated into the first reaction zone (4.0) and the second reaction zone (4 work) simultaneously, and the analyte is separated from the first reaction zone (4 〇) and the second reaction zone, respectively. (4 work) The substance i is reacted, and the test object is contacted with the first reaction zone (4 〇), so that the dry reagent is dissolved and reacted, thereby generating an electrochemical change of the Y-azole from the first reaction zone (4 0 ) The first working electrode (2 〇) and the reference electrode (2 4 ) are in contact with each other, and are connected by the bio-sensing device and receive another test object to contact the second reaction zone (4 i ) to cause the dry reagent to be dissolved and reacted. And, in turn, an electrochemical change occurs. Since the second reaction zone (4 1 ) does not contain an enzyme and an enzyme cofactor, the reaction is a reaction of the interferent, and is not the specificity of the substance to be measured. Reaction 'so the result of the first reaction zone (4 〇) is deducted The reaction zone (41) the result of the measurement, i.e., the amount of concentration of the substance. (d) /f Referring to Figures 4 to 6, which is a second embodiment of the present invention, the test paper includes a substrate (1 2 ) pole (26, 27), at least one reference electrical reaction Zone (42, 43), a middle partition (two - (52). The working electrode (26, 27, /. anti-potential 1 J b 2 7) is set on the substrate (1 9 M296369 2 j and mutual Preferably, the working electrode (26, 27) is a first working electrode (26) and a second working electrode (27), and the reference electrode (28) is disposed on the substrate (1) 0) I is not in contact with the working mine, 6 ϋ ζ 7). The middle partition (3 2 ) is frosted on the substrate (1 〇), the working electrode (26, 27) and the reference electrode ( a part of 2 ^ ), and the middle partition (32) has a groove (3 3 ) ' $ groove (3 3 ) is attached to the end of the middle partition (32), preferably The groove (33) is a tr-shaped type. The reaction zone (42, j is a first reaction zone (42) and a second reaction zone (43), the reaction zone (42) and the second reaction The area (43) is disposed on the horizontal path of the groove 八 3 3 ) in a c-shape, wherein the first a region (4 2 ; two enzymes, an enzyme cofactor, a stabilizer (such as a polymer), and the like, and the second reaction zone (43) contains all the substances except the first reaction, except for the enzyme. In addition, the first reaction zone (4 is connected to the η-working electrode (26) and the reference electrode (2 8)?. and the 5 Hz second reaction zone (4 3 ) and the second working electrode (2 ” The electrode (28) is in contact with each other, that is, the first reaction zone (4, right second, second reaction zone (4 3 )# with a reference electrode (28) and a working electrode (26, 27). (52) a is further in the middle partition (q notch (3 2 ), and the upper partition (52) has a 2) riding gap (53) corresponding to the first reaction zone " : (43), that is, the notch (53) is located at the end of use, and the blood M296369 liquid is inhaled by the end of the ancient + & 1 " type, and the corresponding gap (5 3 ), then the blood enters through the capillary phenomenon. a groove (33) of the middle partition (3 2 ) and reacting with the substances of the first reaction zone (42) and the second reaction zone (43), since the first reaction zone (42) contains an enzyme So can be The substance to be measured in the liquid reacts specifically, but the interfering substance in the blood will react with other substances in the first reaction zone (4 2 ), thereby causing noise interference, and thus the second reaction zone (4 3) The electrochemical change produced is the result of the pseudo-reaction, and the result produced by the first reaction zone (42) can be deducted from the result of the second reaction zone (43). The exact value is measured. In summary, since there are at least two reaction zones, and one of the reactions contains all the substances that react with the substance to be tested, both the substance to be measured or the substance causing the pseudo-reaction will function. In addition, the reaction zone contains all the substances except the enzyme, so if the reaction is not detected, the reaction will be apparent, so the result can be obtained by subtracting the reaction zone without the enzyme, thereby obtaining accurate results. The electrochemical biosensor test paper with the function of eliminating interference can deduct the noise caused by non-specificity, thereby achieving the purpose of rapid detection and accurate measurement. ▲ Although the foregoing examples illustrate the specific facts of the creation, The understanding of any possible amendments and changes does not detract from the spirit and scope of the patent claims below. BRIEF DESCRIPTION OF THE DRAWINGS The first figure is a top view of a first embodiment of an electrochemical biosensing test strip having an interference-eliminating function. A picture shows the upper partition removed and the upper view
M296369 苐一圖係本剑作苐 圖。 苐三圖係本創#货 π ^ ^ J作第二圖將中隔板移開後之上視示意 圖。 1 ^图係本創作具有排除干擾功能的電化學生物感測 ^ 试紙第二種實施例之上視示意圖。 第五圖係本創作第四圖將上隔板移開後之上視示意 圖。 第六圖係本創作第五圖將中隔板移開後之上視示意 圖。 【主要元件符號說明】 (1 〇 ) ( 1 2 )基板 (20)(26)第一工作電極 (2 2 ) ( 2 7 )第二工作電極 (24)(28)參考電極 (3 〇 ) ( 3 2 )中隔板 (31)(33)凹槽 (4 〇 )( 4 2 )第一反應區 (4 1)(43)第二反應區 (5 〇 ) ( 5 2 )上隔板 (5 1 ) ( 5 3 )缺口 12M296369 This picture is a picture of this sword.苐三图系本创# goods π ^ ^ J as the second picture, the middle partition is removed and the top view is shown. 1 ^ Figure This is a top view of a second embodiment of an electrochemical biosensing test strip with interference-eliminating function. The fifth picture is a schematic view of the fourth figure of this creation after the upper partition is removed. The sixth figure is the top view of the fifth panel of this creation after the middle partition is removed. [Description of main component symbols] (1 〇) (1 2) Substrate (20) (26) First working electrode (2 2 ) ( 2 7 ) Second working electrode (24) (28) Reference electrode (3 〇) ( 3 2) Middle partition (31) (33) Groove (4 〇) ( 4 2 ) First reaction zone (4 1) (43) Second reaction zone (5 〇) ( 5 2 ) Upper partition (5 1) ( 5 3 ) gap 12