TWM281174U - Polarized light absorptive biochemical optoelectronic inspecting system - Google Patents

Description

M281174 is suitable for large-scale use. It has become a complex equipment for dyeing, and it is also a means of operation. Its content method and absorption spectrometer are similar to the analytical instrument, and the near-infrared is used for the pre-physiology of color samples. Etc., a spectrometer, water 9, creation instructions (1) [Technical field to which the new type belongs] This creation relates to a biochemical specimen, a specimen requirement for a biochemical polarized light absorption biochemical photoelectric detection characteristic, and dual light The sensitivity of Cheng is superior. [Previous technology] Biochemical detection methods are basically divided into the method of using biosensors to convert reagents into reagents, that is, the quantitative measurement using chemical reagents or degree techniques. The first method has a simple measurement method to achieve in-situ measurement and multi-parameters at the same time with low reproducibility. The second method has good quantification and is widely used in automatic biochemical analyzers. It is a widely used biochemical detection method; its lack of movement, state measurement, and reagents have a biochemical quantity to the environment. Method, fluorescence method, basic instrument using the i / X method, Zhu Zhugou and commonly known as / knife, photometric spectrometer is a long-distance UV spectrometer. Ultraviolet light can be seen in spectrometers. Photoelectric detection systems, especially a system that can detect both polarized light and a simple optical system. '_ Two categories. One is the sensor detection method. The other type of H test paper detects biochemical components, and then uses 2 stools, small devices, fast speed, volume measurement, no reagent contamination, but accurate inspection, because the main body of audiological research is performed in vivo. The photometric method is the material's photochromic method, and these methods are used to reduce the infrared ray quality analysis and

Page 5 M281174

Fourth, the creation instructions (2) chemical inspection, etc., near-infrared spectrometer can be used in food processing industry, manufacturing process monitoring, infrared spectrometer is often used for gas analysis. ^ 的 The characteristics of spectral analysis include: non-invasive, non-destructive, chemical wind discrimination, wavelength flexibility, high sensitivity and fast analysis speed. & Detailed analysis has many advantages and can be applied in a wide range, such as: gas, hospital, health = 1 laboratory, automotive sheet metal, printing coatings, food processing, light-emitting diodes

(Light Emitting Diode, abbreviated as LED), etc., but the current industry penetration rate is not high, the reason may be that the spectrometer's single & expensive, bulky, routine maintenance and wavelength calibration procedures are complicated. Questions There are quite a few types of spectrometers commonly used in biochemical inspections. The shortcomings of large optical analyzers are large and expensive, so I will not go into details here. Miniature spectrometers have many advantages that large spectrometers do not have, such as light weight, small size, fast detection speed, easy to use, can be integrated, and can be manufactured in large quantities at low cost. The vast number of applications. ~ Φ Please refer to the first figure. The basic structure of a traditional miniature spectrometer system is composed of an optical system and an electronic circuit (not shown). The optical system includes at least an optical lens 9 for collecting light, a traditional light source 92 composed of a tungsten filament lamp or a light emitting diode (LED), a beam splitting element 93 composed of a beam splitter or a fiber coupling element, and a microdiffractive element. 94 和 ensors 95. The optical lens 91 used for light collection can collect light radiation into the micro spectrometer system. The numerical aperture of the lens (Numerjcai Aperture, referred to as% Ν · Α ·) must be matched with the light guide fiber used. For tungsten light or LED traditional light, the emission spectrum of source 92 needs to be wide enough and can be located in the absorption of the sensing element 95. M281174 IV. Creation instructions (3) I * = Microspectral spectroscopic element 9 3 is used for separation Via fiber guide-Du Er * Since the light leaves the fiber as divergent light, the micro-spectrum: unit: ot has the ability to separate the spectrum, and it should also have the intensity to converge the light. With this force, the light intensity obtained by the photosensitive element 95 is obtained for each spectrum. ίΐί3 The system performs recording of the spectral intensity and converts it into an analog signal output. The driving circuit and the vanadium front say that it is necessary to supply the timing required for the sensing element. This is the base; Ratio: Digital (a / d) conversion and the overall structure of the function of image transmission ;;: Ang; avoid the use of high-voltage or high-frequency circuit design, traditional large or miniature wells ★ Xianli + specifications of rectangular quartz capacity i (ΐ The test method uses the central * V of a standard biochemical test to find (the car Γ multiplied by the bottom area is-cm square, equal to the fluid (the body must == the synovial fluid of the affected joint, but-like people The synovial fluid in the joints = too much slipping of the joints of the icc joint to avoid injury to the joints). When the amount of extraction is very low (it can not be tested, inspected. Insufficient daily inspection will cause it to be impossible. In addition, due to the many types of specimens , For example: sucrose (66.6 degrees of specific rotation, there are some specimens (see Annex I) (D-rotating substance with specific rotation of 52.5 degrees) 疋 biomass), glucose-91. 9 The left-handed substances of degree) and so on are all partial sugars (determined by optical rotation-like light sources. The measured data disc characteristic substances have a large error if they are similar to the threshold value (or M281174 on page 7). Creation instructions (4) cannot be detected.) The substances with polarized light characteristics also include: gems and minerals in nature (Quartz), human body's diamond spindle, collagen, uric acid crystalline salt, protein, amino acid, sugar, optically asymmetric biomolecules with three-dimensional structure. Because some optical properties of minerals vary depending on the direction, the choice of light waves Absorption and total absorption intensity also change with direction. If you use non-polarized light to detect, the error will be very large (or impossible to detect). For example, for gout patients, the synovial fluid of their joints contains the crystalline component of urate, And the crystals of urate have "polarized light characteristics", but the traditional biochemical photoelectric detection structure does not have a design that can detect biochemical specimens with polarized light characteristics. Therefore, the measured data are accurate and accurate. Quickly determine the patient's condition (the gout is severely severe: degree), not only Gu Yi misjudges, but also cannot give correct treatment. So the disadvantages of the traditional detection system can be listed as follows: [1] · Unable to detect polarized light Characteristics of biochemical specimens. [2] · The required amount of specimens is large. [3] · The optical system is complicated and the price is high. [4] · The sensitivity of single optical path is low. Therefore, It is necessary to develop new technology to solve the above disadvantages. [Creation content] 'The main purpose of this creation is to provide a polarized light absorption biochemical photoelectric detection system that can detect biochemical specimens with polarized light characteristics. 夬 雷 浐 Ji ί : The purpose of ί is to provide a polarized light absorption biochemical photoelectric k-detection system. First, the required sample quantity is small.

M281174 3. Creation description (5) Another purpose of this creation is to provide a kind of polarized light absorption ^ electrical detection system, which has a simple optical system and low price. ^ Another purpose of this creation is to provide a Polarized light absorption photoelectric detection system 'It is a dual optical path design with high sensitivity. It includes a creative department and provides a kind of polarized light absorption biochemical photoelectric detection system. The column container has a predetermined depth of the recessed portion and a predetermined straight: a circular receiving recessed portion for receiving a specimen:-the first end and A second end; a mouthpiece has: a plane mirror, which is arranged near the first end of the cylindrical container, and the light beam reflected thereon is reflected back along the original path; and a collimating lens, which is arranged on the second of the cylindrical container Optical fiber: one-side polarization = splitter, with a polarized beam splitter,-leading nine fibers, one leading-out fiber, and a two-way fiber; the first one: f to generate a predetermined wavelength range and have-initial intensity mutual conversion : A half-wave plate, 'to be used between linearly polarized light and circularly polarized light, a detector to detect the final strength of the received light-a Λ 5 tiger comparison unit to compare the guide and finally The difference between the intensity and the initial intensity; ν represents the light received by the fiber, and the lead-in fiber will split the polarized light with linear polarization characteristics and pass through the polarized beam splitter.

Page 9 M281174 Creative Instructions (6) The second beam with wired polarization characteristics, 3 The second beam enters the collimator through the bidirectional fiber, and diffuses into the collimator lens when it is derived—close to the diameter of the recess, with a line The third light beam with polarization characteristics; ° Hirth—After the light beam enters and exits the cylindrical container, it becomes a fourth light beam with linear polarization characteristics. The fourth light beam passes through the quarter wave plate and becomes a circular polarization characteristic. The fifth beam; after the fifth plane mirror, it becomes a sixth beam with circular polarization characteristics and radiates. After red, one wave plate of four blades becomes a seventh beam with linear polarization characteristics. The micro-container is led out into a light beam with linear polarization. The eighth light beam passes through the collimator lens and becomes a light beam. The ninth light beam with linear polarization characteristics is split along the bidirectionally polarized light. At that time, the reflection of the tenth t beam with linear polarization characteristics is turned to the lead-out fiber and reaches the detector, and then the absorption coefficient of the specimen in the cylindrical container is calculated by the frame; The correction generated by the quarter-wave plate calculates the concentration of the specimen. The above-mentioned purpose and advantages of this monthly creation are not difficult—the detailed description of pt and the accompanying drawings I have gained a deep understanding. The following examples are used: : Please refer to the second and third pictures. This creative department is a pan-biochemical photoelectric detection system, which includes: a circular polarized light absorption type,-a collimating lens 3G,-a polarizing beam splitter 1G ... a flat mirror 20 detector 60 and A signal comparison unit 70. °° ϋ, —light source 50, one inspection

M281174

A second end 10B. The plane mirror 20 is disposed near the first end 10A of the cylindrical container 10 to reflect the light beam projected thereon by the light source 50 back along the original path. The collimating lens 30 is provided in the cylindrical container 10. Outside the second end 10β, there is a polarization beam splitter 41, a bidirectional optical fiber 44, and a quarter wave plate 45. The plane mirror 20 and the polarizing beam splitter 40 are disposed on the plane mirror 20, There are M lead-in optical fiber 42, a lead-out optical fiber 43, and a wave plate 45, wherein the quarter-wave plate cylindrical container 10 is used for phase conversion between linearly polarized light and circularly polarized light. The light source 50, The detector 60 is used to generate a first light beam L1 having a predetermined wavelength range and having an initial intensity; the detector 60 is used to detect the final intensity of the light received by the lead-out fiber 43; the signal comparison unit 70 'is used to compare the The difference between the final intensity of the light received by the optical fiber 43 and the initial intensity; thereby, the first light beam L j (for example, P wave) with linear polarization characteristics emitted by the light source 50 is passed through the introduction fiber 42 Is introduced (see the fourth figure) and penetrates the polarizing beam splitter 40 The polarized light beam splitter 稜鏡 41 becomes a second light beam L2 with a wired polarization characteristic, and then enters the collimator lens 30 through the bidirectional fiber 44, and diffuses into a close to the cylinder when leaving the collimator lens 30.

Page 11 M281174 IV. Creation instructions (8) The third beam L3 with linear polarization characteristics of the diameter D of the recessed portion 10 (theoretically, its intensity is substantially equal to the second beam L2 and is still a P wave); After the three beams L3 enter and re-export the cylindrical container 10, they become a fourth beam with linear polarization characteristics (theoretically, part of the beam is deflected and absorbed by the sample 12 linear polarization, so the fourth beam L4 is smaller than the first beam. The three beams L3 are weak but still p-waves). After the fourth beam L4 passes through the quarter wave plate 45, it becomes a fifth beam L 5 with circular polarization characteristics. The fifth beam L 5 is irradiated to After the plane mirror 20 ′ becomes a sixth beam L 6 which still has a circular polarization characteristic, and after entering into the __ and deriving the quarter wave plate 45, it becomes a seventh beam L 7 having a linear polarization characteristic. (For example, S wave) 'The youngest seven beams L 7 pass through the cylinder volume 10 and become an eighth beam L8 with linear polarization characteristics (theoretically due to the partial beam once again the subject's 2 linear polarization deflection and Absorption, so the eighth beam L8 is weaker than the seventh beam L7 'but still S wave)' the first After passing through the collimating lens 30, the eight beams 18 become the ninth beam 9 with a concentrated beam and linear polarization characteristics (theoretically, the intensity is equal to the eighth beam L8, and it is still an S wave). Nine beams L9 return to the polarization beam splitter 稜鏡 41 along the bidirectional fiber 44. Due to the difference of 90 degrees from the second beam L2, they cannot penetrate the polarization beam splitting prism 4 1 ′ and become linearly polarized after being reflected. The tenth beam ^ 1 〇 # (which is an S wave), and reaches the detector 60 through the lead-out optical fiber 43, and the signal comparison unit 70 calculates the absorption of the specimen 12 in the cylindrical container 10. The coefficient and the concentration of the sample 12 can be converted by adjusting the correction deflection angle of the quarter-wave plate 45. Theoretically, when the third light beam L3 passes through the specimen 12,

12) " --- M281174

12 the concentration is thicker, the third beam] ^ 3 (still s-wave) has a larger deflection angle't can be adjusted (such as manually turning the quarter-wave plate 45 angle) to the quarter-wave plate 45 correct the deflection angle, return the ninth light beam L9 (still s Λ wave) to the polarized light beam splitter 1 41, and change to a ten-beam L10 with linear polarization characteristics and be reflected and redirected to the lead-out fiber 43. Arrive at the detector 6 00. Therefore, the signal comparison unit 70 calculates the absorption coefficient of the detector 2 in the cylindrical container, and the quarter-wave plate 45 corrects the deflection angle. It can be changed to • I or corresponding The concentration of the sample 12 is obtained. The path of the light beam in this creation has been detailed in the previous paragraph, and the basic principle of this creation is to detect the attenuation of the visible light polarized light quasi-i light wave or the angle of polarization deflection (i.e. After passing through the specimen 2-a person will attenuate once) 'to know the light absorption or specific rotation of the specimen 2 with polarization characteristics. The measurement object of this system is the colorimetric substance or specific optical rotation of the sample i 2 & biochemical reaction with polarization characteristics. The color depth or specific optical rotation of the colorimetric substance solution has a certain relationship with its concentration. Deeper or greater than the optical rotation, lighter or smaller than the optical rotation when the concentration is small, you can measure the concentration of the specimen to be measured by measuring the depth or specific rotation of the color of the colored substance. 2 The concentration relationship table of biochemical components is used to measure the concentration of the biochemical components contained in the specimen 12. The specific optical rotation of the substance is measured with a polarimeter, and the specific optical rotation of the solution is shown to be related to the optical rotation capacity, solution properties, solution concentration, sample tube length, and temperature of the substance contained in the solution. For application examples, relative relationships and calculation methods, please refer to Annex 1.

Page 13 M281174

Immunoreactive antigens or antibodies in biochemical tests are often connected with gold colloids as coloring substances. When the concentration of the color-changing substance (Blue Latex) changes, the voltage of the photo-sensing unit is also changed. The voltage has a certain relationship with the amount of concentration change. The amount of concentration change can be directly measured by a digital trimeter. Obtained by measuring the analog output terminal voltage. The coloring matter (B 1 u e L a t e X) was obtained through experiments; the relationship between the degree change and the voltage was consistent with "Beer-Lambert's law". The preferred specifications for some of the components in this case are as follows: Each optical fiber (including the lead-in fiber 4 2, the lead-out fiber 4 3, and the bidirectional fiber 44) is a multimode plastic fiber with a concave diameter of 1.00mm and a numerical aperture of 0.44; The light source 50 is a light-emitting diode, and its spectrum is as shown in the fifth figure, and the peak wavelength is 590 nm; the detector 60 is a shi photo detector (Si PIN photo detectQr0, < the output power after the optical fiber is combined is 5 〇 # W. The diameter D of the concave portion of the circular accommodating concave portion 11 of the cylindrical valley device 10 is 5 centimeters (5 cm), and the depth D of the concave portion is about 0 · ΐ microns (0.0 00 1 cm). The volume is only (3.14x52) / 4x〇〇〇〇〇〇i = 0.02 cc, which is about two thousandths of CC ° In summary, the advantages and effects of this creation can be summarized as ... [1]. Detectable Specimens with polarized light characteristics. For polarized specimens (such as uric acid crystal salt, collagen, etc.), since the original uses linearly polarized light to penetrate the specimen, it can be used for polarized specimens. The specimen is tested to accurately calculate the specific rotation (yield) of the specimen in the container, so that it can be followed up. Analysis, analyzing or diagnosis. Show

Page 14 M281174 Fourth, the creation instructions (11) &quot; &quot; &quot; — 1 [2] · The amount of specimen required is very small. The specimens required for the cylindrical container of this creation are positive //, about two-thousandths cc, about one-fifth of the traditional minimum specimen volume of one cc, in other words, a very small number of specimens can be used. Testing has wider applicability in medical or biochemical tests. Manufacturing [3] · The optical system is simple and the price is low. The system of this creation is simple and can be used for special models of specimens, such as g = patient specimens for gout patients, octapolarized light characteristic crystal concentration detectors, commercial promotion [4], high sensitivity of each light. The original light beam penetrating the subject IΛ A @ β A Α double-first design will increase the degree. m ... people k become twice, therefore, the overall sensitivity is only a detailed description of the present invention from the preferred embodiment of the embodiment, for this reality and scope. The early amendments and changes do not depart from the essence of this creation; from the above Note 4M, the above-mentioned second month can be achieved: the artist can understand the patent application for this creation. The purpose is actually in compliance with Regulation I of the Patent Law: [Annex] Annex 1: Annex I: Measuring the specific optical rotation of a substance by Gyro-Medium Nine Meters. <Examples and related plans

Page 15 M281174 m Schematic description [Schematic and early explanation] The first picture is a traditional micro-second picture is the third picture of this creation The fourth picture is the fourth picture of the creation The fifth picture is the creation of the [Symbol element of the diagram says 10 cylindrical container 10B second end 12 specimen 30 collimator lens 41 polarized light beam splitter 43 lead fiber 45 quarter wave plate 6 0, detector 9 1 optical lens 9 3 beams Element 9 5 Photosensitive element D Concave diameter L2 Second beam L4 Fourth beam L6 Sixth beam L8 Eighth beam L10 Tenth beam type spectrometer Basic structure of the optical system Unintended structure of the optical system Schematic diagram of the light source without intending to simplify the beam path] 1 0 A first end 11 circular receiving recess 2 plane mirror 4 0 polarizing beam splitter 42 introducing optical fiber 4 4 bidirectional fiber 5 0 light source 7 0 signal comparison unit 9 2 Traditional light source 94 Micro-diffractive element Η Depth of recessed portion L1 First beam L3 Third beam L5 Fifth beam L7 Seventh beam L9 Ninth beam

Page 16

Claims (1)

M281174 5. The scope of the patent application includes: and a predetermined concave body, the cylinder contains a polarized light absorption type biochemical photoelectric detection system, a cylindrical container, a circular receiving recess with a predetermined depth of the recessed portion, for containing A detector is provided with a first end and a second end; a plane mirror is provided near the first end of the cylindrical container to reflect the light beam projected thereon back along the original path; a collimating lens is provided at the Outside of the second end of the cylindrical container; a polarizing beam splitter having a polarizing beam splitter, an introduction fiber, an exit fiber, and a bidirectional fiber; a light source 'for generating-a predetermined wavelength range and-the first A light beam; a quarter wave plate's mutual conversion; used as a detector between linearly polarized light and circularly polarized light to detect the final intensity of the received light;: a signal comparison unit 'for comparison The difference between the final intensity of the derived optical fiber and the initial intensity; In this way, the introduction fiber introduces a first light beam with linear polarization characteristics and penetrates the polarization beam splitter of the polarization beam splitter into a second light beam with linear polarization characteristics, and the second light beam passes through the The bidirectional fiber enters the collimating lens, and when the collimating lens is led out, it diffuses into a third light beam with linear polarization characteristics close to the diameter of the recess; after the third light beam enters and exits the cylindrical container, it becomes a linear polarization characteristic A fourth beam, the fourth beam passing through the quarter wave plate into a fifth beam having a circular polarization characteristic; the fifth beam Page 17 M281174 V. Application scope 2 After being irradiated to the plane mirror, it becomes a beam and reflects. After passing through the seventh beam of the quarter-vibration characteristic, the first beam becomes a ninth beam with linear polarization after passing through the collimating lens. At 稜鏡, it becomes linearly polarized to the lead-out fiber and reaches the unit to calculate the concentration of the sample produced by adjusting the quarter-wave plate in the cylindrical container. The detection system according to item 1 of the scope of patent application, wherein the cylindrical flat plate is engraved with a circular flat receiving recess having a diameter between 0.05 and 1.5 micrometers. The detection system according to item 1 of the scope of patent application, wherein the intensity of the third light beam is substantially greater than the intensity of the fourth light beam than the eighth light beam than the seventh light beam, the ninth light beam, and the second light and the polarization The light splits directly and the intensity of the ninth beam is roughly one of the sixth light with a circular polarization characteristic. After the wave plate becomes a seventh beam with a linear deflection, it enters and leads to the eighth beam with a small amount of capacitance. The bidirectional optical fiber with linear polarization characteristics in the beam concentration returns to the tenth light beam with polarized light splitting characteristics and is reflected to the detector, and then the absorption coefficient of the specimen is compared by the signal; and the deflection angle can be corrected by the matching The polarized light absorption biochemical photoelectric device described in conversion is a rectangular transparent plastic groove-shaped circular receiving part, which is 4 to 6 centimeters, and the polarized light absorption biochemical photoelectric system described in the concave part has polarized light. And the third beam is weak; weak; the beams differ by about 90 degrees so they cannot be reflected; U is equal to the eighth beam.