M274370 八、新型說明： 【新型所屬之技術領域】 本創作係有關於一種生物晶片結構，尤其是指一種不 ，可同時適用於檢測職*腿,病毒，且於操作流程上更 ^夬速、加熱及冷卻過程更為迅速，於操作使社更為簡 f ’而能更增其實用功效特性之生物晶片創新結構設計 者。 【先前技術】 鲁 & ’近年紐著半導職錢㈣微機t技術的蓬勃 發展，利用微機電技術所製作出的微晶片是一新颖而有潛 力的產業。而生物晶片就是最被看好具有未來明星產業之 一。生物晶片是指泛指採用半導體策略應用於生物性分析 賴立的微小化裝置，也就是將傳統大型的分析、檢驗等 H具’料微小化、積體切及平行^卫化。生物晶片通 常以矽晶片、玻璃或高分子為基材（Substrate)，在基材 上製作出結構或是控制電路，用來檢測分析生物性分子或 鲁更進一步的應用。生物晶片的體積小、反應快速並且能夠 平行分析大量生物資訊，因此適用於生化處理、分析、檢 驗、新藥開發及環境監測等用途上…般而言可以再將生 物晶片分成微陣列晶片（Micro-array)與處理型晶片 (lab-on-a-chip)。微陣列型生物晶片是在晶片基質上整 齊的固定、排列大量生物探針（DNA或蛋白質），'透過 DNA-MA雜交反應或是蛋白質間特異性結合，用以進行樣 品分析或檢驗。 其中’-般應㈣生物晶>；進行DM(去氧核酸）病毒 M274370 〔如··腺病毒、小DNA病毒、泡疹病毒、痘病......等〕之 檢測時，其皆是採用 PCR〔p〇iymerase chain Reaction， 聚合酶連鎖反應〕方式，主要係於生物晶片（2)上設置有 凹槽（21)，且對應該凹槽（21)分別設有加熱器（22)及檢測 器（23)〔如第三圖所示〕，以於該凹槽（21)中滴入待檢測 液後，利用加熱器（22)先將待檢測液加熱至g5度使雙股 DM變成單股DNA，令待檢測液降溫至55度使起始物接上 單股DM，再將待檢測液升溫至72度使起始物長成雙股 • dm，以能獲得所要得到之DNA序列片段；然而，由於該 PCR方式僅能針對DNA病毒進行檢測，於是即又有 RT-PCR(Reverse Transcription-PCR，反轉錄一聚合酶連 鎖反應）方式之研發。 該RT-PCR方式係針對RNA(核糖核酸）病毒〔如：腸性 病毒、流感病毒、冠狀病毒、惡性腫瘤......等〕進行拾制 其主要係如第四圖所示，於生物晶片（3)上設有凹槽M274370 8. Description of the new type: [Technical field to which the new type belongs] This creation relates to a kind of biochip structure, especially a kind of no, which can be applied to the detection of legs, viruses, and more in the operation process. The heating and cooling process is faster, and the operation of the innovative structure designer of the biochip is simpler and easier to operate. [Previous technology] Lu & ’In recent years, the semi-conductor Qian Qian's microcomputer technology has developed vigorously. The microchip manufactured using micro-electromechanical technology is a new and promising industry. And biochip is one of the most promising industries with a future star. Biochip refers to the miniaturization device that uses semiconductor strategies for biological analysis. It means miniaturization, integration, and parallelization of traditional large-scale analysis and inspection equipment. Biochips usually use silicon wafers, glass, or polymers as substrates. Structures or control circuits are fabricated on the substrates to detect and analyze biological molecules or further applications. Biochips are small in size, fast in response, and capable of analyzing a large amount of biological information in parallel, so they are suitable for applications such as biochemical processing, analysis, inspection, new drug development, and environmental monitoring ... Generally, biochips can be further divided into microarray chips (Micro- array) and processing-type wafers (lab-on-a-chip). Microarray-type biochips are a large number of biological probes (DNA or protein) that are immobilized and arranged on the wafer matrix. They are used for sample analysis or inspection through DNA-MA hybridization reactions or specific binding between proteins. Among them, '-Bian Ying's biological crystal> When testing for DM (deoxyribonucleic acid) virus M274370 [such as adenovirus, parvovirus, herpes virus, pox ... etc.], All adopt PCR (p0yymerase chain reaction) method, which is mainly provided with grooves (21) on the biochip (2), and heaters (22) corresponding to the grooves (21). ) And detector (23) [as shown in the third figure], after the liquid to be detected is dripped into the groove (21), the heater (22) is first used to heat the liquid to be detected to g5 degrees to make double strands DM becomes single-stranded DNA, cool the liquid to be detected to 55 ° C and connect the single-stranded DM to the starting material, and then raise the liquid to 72 ° C to make the starting material grow into double-stranded • dm to obtain the desired DNA sequence fragments; however, since this PCR method can only detect DNA viruses, there is also the development of RT-PCR (Reverse Transcription-PCR) method. This RT-PCR method is for RNA (ribonucleic acid) viruses [such as: enterovirus, influenza virus, coronavirus, malignant tumor, etc.]. The main system is shown in the fourth figure. Groove on the biochip (3)
(31) ，同時對應該凹槽（31)分別設有加熱器（32)及檢測 (33)，以令待檢測液滴入該凹槽（31)中後，先利用加埶^ (32) 將待測液加熱至37—4Q度，再將待檢測液加熱至； 度’令待檢測液降溫至55度，再將待檢測液升溫至U 即能獲得所需之RNA序列。 又 J，上述不論係PCR方式或RT—pcR方式雖可達 別檢測DNA或RNA病毒之預期功效，但於其實際操作= 使用上卻發現’其兩者减於實錢作上皆較為】 便’且於等待加溫、降溫過程中亦極為耗時，而在 使用上存有極大不便之處尚待改進。 呆 【新型内容】 6 M2.74370 / 、本創作之生物晶片結構，其主要係於生物晶片 上凹設 幵成有々IL道，且於該流道分別設有四組不同溫度之第一〜 第四區段，並對應各區段分別設有加熱器及控制器，以於 奴進仃RT-PCR方式檢測RNA病毒時可將待檢測液由第一 區段之滴入口處滴入，欲進行pcR方式檢測疆病毒時將 待檢測液由第二區段之滴入口處滴入，令該待檢測液流經 各區#又進行加溫、降溫，而能於第四區段末端獲得所需之 RNA或DNA序列；藉此，以能更為迅速即達加熱及冷卻效 .果，而於操作使用上更為簡便。 【實施方式】 首先，請參閱第一圖所示，本創作主要係於生物晶片 (1)上凹設有流道（11)，且該流道（11)分別分為第一區段 (12)、第二區段（13)、第三區段〇4)及第四區段（15)，並 於第一區#又（12)及弟二區段（13)分別設有滴入口（I?〗）、 (131)，同時對應該第一區段（12)〜第四區段（π)皆各設 有加熱器（16)及檢測器（17)，以能令該第一區段〇2)保^ • 在37-40度、該第二區段（13)保持在95度、該第三區段 (14)保持在55度、該第四階段（15)保持在72度。 又(31). At the same time, a heater (32) and a detection (33) are respectively provided for the groove (31), so that after the liquid to be detected is dripped into the groove (31), first use 埶 ^ (32) The test solution is heated to 37-4Q degrees, and then the test solution is heated to degrees; the temperature of the test solution is cooled to 55 degrees, and the test solution is heated to U to obtain the desired RNA sequence. Also J, although the above PCR method or RT-pcR method can achieve the expected efficacy of detecting DNA or RNA viruses, but in its actual operation = use, it is found that 'both of them are less than real money.] 'And it is extremely time-consuming while waiting for heating and cooling, and there are great inconveniences in use that need to be improved. [New content] 6 M2.74370 / The biochip structure of this creation is mainly formed on the biochip with recessed IL channels, and there are four groups of different temperatures at the first ~ The fourth section is provided with a heater and a controller corresponding to each section, so that when the RNA virus is detected by the RT-PCR method, the test solution can be dripped from the drip inlet of the first section. When performing pcR detection of Xinjiang virus, the liquid to be detected is dripped from the drop inlet of the second section, so that the liquid to be detected passes through each zone # and then heated and cooled, so that it can be obtained at the end of the fourth section. The required RNA or DNA sequence; by this, the heating and cooling effect can be achieved more quickly, and the operation and use are simpler. [Embodiment] First, please refer to the first figure. This creation is mainly based on the flow channel (11) recessed on the biochip (1), and the flow channel (11) is divided into the first section (12). ), The second section (13), the third section 04) and the fourth section (15), and drip ports (1) (# 12) and the second section (13) are provided with drip inlets ( I?〗), (131), corresponding to the first section (12) to the fourth section (π) are provided with a heater (16) and a detector (17), so that the first section Segment 〇2) Guarantee ^ • At 37-40 degrees, the second section (13) is maintained at 95 degrees, the third section (14) is maintained at 55 degrees, and the fourth stage (15) is maintained at 72 degrees . also
如此一來，當欲控制RNA病毒時可將待檢測液由該生 物晶片（1)流道（11)第一區段（12)之滴入口（121)處滴 入，欲檢測DNA病毒時則可將待檢測液由該生物晶片 流道（11)第二區段（13)之滴入口（131)處滴入，令該待檢 測液分別流經流道（11)之各區段，使該待檢測液於^區^ 中經加熱、降溫，而即能於生物晶片（1)流道（n)之第= 區段（15)獲得所需之RNA或DNA序列者。 W 藉由以上所述，該元件之組成與使用實施說明可知， M274370 本創作與習用相較之下，由於本創作於生物晶片上利用流 道之設計，且針對所需之不同溫度將流道分為各不同溫度 之區段’使得於待檢測液滴入流經生物晶片流道之各區段 時，能於該流道中經加熱、降溫，以能在流道第四區段中 獲得所需之RNA或DNA序列，不僅於加熱、降溫過程中更 為迅速，且在整體操作使用上更為簡易、便利、快速，而 更增其實用功效特性者。 綜上所述，本創作實施例確能達到所預期之使用功 > 效，又其所揭露之具體構造，不僅未曾見諸於同類產品 中，亦未曾公開於申請前，誠已完全符合專利法之規定與 要求，爰依法提出新型專利之申請，懇請惠予審查，並賜 准專利，則實感德便。 M274370 【圖式簡單說明】 第一圖：本創作之結構示意圖 第二圖：本創作之結構剖視示意圖 第三圖：習用之結構示意圖 第四圖：另一習用之結構示意圖 【主要元件符號說明】 (1) 生物晶片 (11) 流道 (12) 第一區段 (121) 滴入口 (13) 第二區段 (131) 滴入口 (14) 第三區段 (15) 第四區段 (16) 加熱 (17) 檢測器 (2) 生物晶片 (21) 凹槽 (22) 加熱器 (23) 檢測器 (3) 生物晶片 (31) 凹槽 (32) 加熱器 (33) 檢測器In this way, when the RNA virus is to be controlled, the liquid to be detected can be dripped from the drip inlet (121) of the first section (12) of the flow channel (11) of the biochip (1), and when the DNA virus is to be detected, The liquid to be detected can be dripped from the drip inlet (131) of the second section (13) of the biological wafer flow channel (11), so that the liquid to be detected flows through each section of the flow channel (11), so that The liquid to be detected is heated and cooled in the region ^, and the required RNA or DNA sequence can be obtained in the (=) section (15) of the flow channel (n) of the biochip. W According to the above, the composition and use of this component can be seen. M274370 is compared with the conventional one. Because this creation uses the design of the flow channel on the biochip, and the flow channel is designed for the different temperatures required. Divided into sections with different temperatures, so that when the liquid to be detected enters each section flowing through the flow path of the biochip, it can be heated and cooled in the flow path, so that it can be obtained in the fourth section of the flow path. The required RNA or DNA sequence is not only faster in the process of heating and cooling, but also simpler, more convenient and faster in overall operation and use, and it has more practical functions. In summary, this creative embodiment can indeed achieve the expected use function > and the specific structure disclosed is not only not seen in similar products, nor has it been disclosed before the application. It has fully complied with the patent. According to the provisions and requirements of the law, if you apply for a new type of patent in accordance with the law, and you are kindly requested to review it and grant the patent, it is very convenient. M274370 [Brief description of the diagram] The first picture: the schematic diagram of the structure of this creation. The second diagram: the cross-sectional diagram of the structure of this creation. The third diagram: the diagram of conventional structure. The fourth diagram: the diagram of another conventional structure. ] (1) Biochip (11) Flow channel (12) First section (121) Drip inlet (13) Second section (131) Drip inlet (14) Third section (15) Fourth section ( 16) Heating (17) Detector (2) Biochip (21) Groove (22) Heater (23) Detector (3) Biochip (31) Groove (32) Heater (33) Detector