TWM273348U - Micro-separator for human blood - Google Patents

Micro-separator for human blood Download PDF

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TWM273348U
TWM273348U TW93219791U TW93219791U TWM273348U TW M273348 U TWM273348 U TW M273348U TW 93219791 U TW93219791 U TW 93219791U TW 93219791 U TW93219791 U TW 93219791U TW M273348 U TWM273348 U TW M273348U
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blood
tank
separation
micro
scope
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TW93219791U
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Chinese (zh)
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Der-Ren Kang
Gwei-Chu Yang
Ker-Jer Huang
Zhi-Jun Zhan
Hsien-Ming Wu
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Chung Shan Inst Of Science
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M273348 四、創作說明(1) 〈新型所屬技術領域〉 本創作是一項新的血液血球血清微分離元件設計,它設計 在一如光碟片大小的材質上,利用如光碟機式的轉動驅動 器所產生之離心力,結合流體及載體材質間表面張力所形 成的閥門來控制流體流動的時序,具有結構簡單、體積 小、無可動件等優點,易與相關的微流體元件作整合。 〈先前技術〉 整個微機電的發展,目前國際上就奈米/微機電技術之應用 及主要發展領域大致可分為··生化微機電(Bio-MEMS)、 微波微機電(RF-MEMS)、光學微機電(Optical-MEMS) 等,其中由於一連串生物科技的進展,複製羊、牛等新聞 再再地呈現世人的眼前,顯示未來數年間生物科技絕對是 科學界發展中的一主軸;尤其是人類基因體定序計劃( Human genome pro ject)在科學界是一項吸引眾人目光的 偉大工程,引發的新一波生化科學研究,生化系統上的應 用發展更受到市場殷切的期盼。 1 990 年初期,Andreas Manz、Jed Harrison 等人首先製作 出具有迷你型化學分;f斤系統的微型晶片,顯示出它具有較 傳統方式消耗少的檢體量、更便宜、更快速的優點;這些 技術原本發展用來作生物學上的分析(biological analysis ),但後來也陸續被用在傳統化學免疫分析 (classical chemical assays)、環境分析、材料量測等, 而且它僅需少量容積及快速的反應的優點’更可以藉由較 精確且快速的流體注入、較佳的中間媒介物或產物移除M273348 IV. Creation Instructions (1) <New Technical Field> This creation is a new blood cell serum micro-separation element design. It is designed on a material the size of an optical disc, using a rotary drive such as an optical disc drive. The generated centrifugal force, combined with the valve formed by the surface tension between the fluid and the carrier material to control the timing of fluid flow, has the advantages of simple structure, small size, no moving parts, etc., and is easy to integrate with related microfluidic components. 〈Previous technology〉 The development of the entire micro-electromechanical industry, at present, the application and main development fields of nano / micro-electromechanical technology in the world can be roughly divided into ... Bio-MEMS, Microwave Electro-Mechanical (RF-MEMS), Optical micro-electromechanical (Optical-MEMS), etc., due to a series of advances in biotechnology, copying news such as sheep and cattle and presenting them to the world again and again, showing that biotechnology is definitely a major axis in the development of the scientific community in the next few years; especially The human genome project is a great project in the scientific community that has attracted the attention of all people. It has triggered a new wave of biochemical scientific research. The application and development of biochemical systems has been eagerly anticipated by the market. In the early 1990s, Andreas Manz, Jed Harrison, and others first produced mini-chips with a mini-chemical system; the f-chip system showed that it has the advantages of less sample volume, cheaper, and faster than the traditional method; These technologies were originally developed for biological analysis, but later they have also been used in traditional chemical immunoassays, environmental analysis, material measurement, etc., and they only require a small volume and fast Advantages of the reaction 'can be removed by more accurate and rapid fluid injection, better intermediate vehicle or product removal

第7頁 M273348 四、創作說明(2) ' 性、較佳的反應變因控制性等來控制傳統反應器所難以達 ,的化學反應控制。在這些微型裝置裡,微流體控制系統 是厂項相當關鍵的技術,它提供了少量檢體、自動化、反 應迅速、平打處理、可拋棄式等優點,發展更複雜、廣泛 使用的流體處理系統,深入探討微流體原理現象已是一盔 用置疑的問題。 一理想的微流體系統必須具備有完整的分析能力,由樣品 的注入(包括樣品儲存與注入)、前處理(包括濃縮、混 合、稀釋及反應)至最後的分析。典型的樣品流量由 nuc^roliters至pic〇Hters,由於流量很少,通常需要特殊 形態的流體裝置來輪送流體。整合各項微流體裝置如微注 入 '微幫浦、·微閥、微混合器、微反應器等形成一縮小型 的为析實驗至,成為各研究單位的共同目標與方向;即所 謂的微型分析系統(VTAS)或稱Lab 〇n a chip的設計概 念0 綜觀目前市場上已存在的生物晶片而言,微流體技術平台 以'泳(ele.Ctroosmosis )、電滲透(electr〇ph〇resis ) …等電動力驅動(electrokinetic )的方式為主流也最為 成熟,各家代表性產品所示。分析電動力驅動技術的缺 點如下表所示; 、 優點:1 ·易於作流體控制。 如分子分離、混合、 2 ·可以執行相關生化處理, 流量控制等。 . 3·可以在玻璃、石英、聚合物等材質上製作。Page 7 M273348 IV. Creative Instructions (2) 'Control of chemical reactions that are difficult to achieve with traditional reactors, such as better control of reaction variables. In these micro-devices, the microfluidic control system is a very key technology for the factory project. It provides the advantages of a small number of specimens, automation, rapid response, flat processing, and disposable type. It develops more complex and widely used fluid processing systems. In-depth discussion of the microfluidic principle phenomenon has been a doubtful question for helmets. An ideal microfluidic system must have complete analytical capabilities, from sample injection (including sample storage and injection), pretreatment (including concentration, mixing, dilution, and reaction) to final analysis. The typical sample flow rate is from nuc ^ roliters to picoHters. Because the flow rate is small, a special shape fluid device is usually required to rotate the fluid. Integrate various microfluidic devices such as micro-injection, micro-pumps, micro-valves, micro-mixers, micro-reactors, etc. to form a reduced analysis experiment and become the common goal and direction of various research units; the so-called micro- Design concept of analysis system (VTAS) or Lab 〇na chip. 0 Looking at the existing biochips on the market, the microfluidics technology platform is based on ele.Ctroosmosis and electro-osmosis. The method of isokinetic power drive (electrokinetic) is the mainstream and the most mature, as shown by various representative products. The shortcomings of the analysis of electric power drive technology are shown in the following table; Advantages: 1 Easy to control the fluid. Such as molecular separation, mixing, 2 · Can perform related biochemical processing, flow control, etc. . 3. Can be made on glass, quartz, polymer and other materials.

M273348 四、創作說明(3) 缺點:1 ·易受流體的物化特性(如離子強度、PH值等)的 影響。、 2·需要高電壓,容易造成生化檢體的破壞。 3 ·僅適合輸送連續流體。 4 ·無法在寬流道輪送高流量(大於1微升/秒)。 =於電動力驅動方式具有上述缺點,碟片式微流體處理平 =的架構乃因應而生,其主要架構如第一圖所示,此一平 :技術疋在·傳統CD大小的碟片上製作出微流體處理元件, =^主要是利用離心力,所以不需要任何驅動元件,來驅 流體的流動,因此設計得宜的話結構相當簡單,依其特 性具有下列優點; 1.低電源及空間的需求。 2 ·對所使用流體的物化性不敏感。 3·允許流體中有氣泡存在;^ 用各種流道寬度及流量(5 nl/sec〜01 ml/sec )的 ^ ,可以配合需求作高密度且平行化的設計。 量:Γ執行生化檢測所需的分離、混合、分流、加埶、流 篁控制等機制。 ”、、 創作C流體系統具有相當的發展潛力,所以本 因為:在此一發展平台上開發一血液分離元件, 便由檢驗流程的第一步驟就是血液分離,以 血液裡的血 &gt;月篩選出可能的疾病。 〈新型内容〉 本創作的主要目的在提供在碟片式微流體系統上所使M273348 Fourth, creation instructions (3) Disadvantages: 1 susceptible to the physical and chemical characteristics of the fluid (such as ionic strength, pH value, etc.). 2. High voltage is required, which may easily cause damage to biochemical specimens. 3 · Only suitable for continuous fluids. 4 · High flow rate (greater than 1 microliter / second) cannot be delivered in a wide runner. = The electric drive method has the above disadvantages. The disc type microfluidic processing level = The structure is born from the response. The main structure is shown in the first figure. This level is flat: technology is produced on a traditional CD-size disc. The microfluidic processing element mainly uses centrifugal force, so it does not need any driving element to drive the flow of fluid. Therefore, if designed properly, the structure is quite simple and has the following advantages according to its characteristics: 1. Low power and space requirements. 2 · Not sensitive to the physical and chemical properties of the fluid used. 3. Allow the existence of air bubbles in the fluid; ^ With a variety of channel widths and flow rates (5 nl / sec ~ 01 ml / sec), ^ can be designed to meet the needs of high density and parallel design. Quantities: Γ performs the separation, mixing, shunting, adding, and flow control mechanisms required for biochemical detection. The creation of the C fluid system has considerable development potential, so this is because: the development of a blood separation element on this development platform, the first step in the inspection process is blood separation, and the blood in the blood &gt; month screening Possible diseases. <New content> The main purpose of this creation is to provide a disk microfluidic system

第9頁 M273348 四、創作說明(4) 用的新型血液中血球血清的分離元件,藉著離心力來驅動 不需任何可動件,就可以將微小流量的血液依照所需來進 行分離,不佔太大體積,可與其他微流體元件整合,免除 其他型態阻塞、效率不佳等問題。 〈實施方式〉Page 9 M273348 IV. Creative Instructions (4) The new type of blood cell serum separation element used in the blood is driven by centrifugal force without any moving parts. It can separate the small flow of blood as required without occupying too much space. Large volume, can be integrated with other microfluidic components, eliminating other types of blockage, poor efficiency and other problems. <Embodiment>

此項碟片式生化感測技術的研發國外自1 9 9 0年左右即投入 至今,直到近一、兩年開始有突破性的進展,陸續有產品 上市’技術領先廠商主要有三家分別為瑞典Gyros、美國 Tecan及Burstein等,其中有關微流體技術的專利有 US4940527 、US5006749 、US5061381 、US5122284 、 US5171695 、US5173193 、 US5173262、 US5186844 、 US5242606 、US5242803 、US5252294 、US5275016 、 US5304348 、 US5304487 、 US5336181 、 US5368704 、 US5403415 、US5409665 、US5413732 、US5426032 、 US5431303 、US5432009 、US5457063 、US5472603 、 US5478750 、 US5496520 &gt; US5518930 、US5590052 、 US5591643 、US5599411 、 US5624597 、 US5639428 、 US5693233、US6 1 43248、US6302 1 3 4 等,分布的年代由 1990 至200 1年之間,其中以US6302 1 34的專利内容最為完整,文 中針對血液分離也提供了兩種具體實施例,如第一〜十一 圖所示;圖中顯示出具體實施例一、二的血液分離操作中 血液流動的狀況,其中兩者的設計有一項共通點,那就是 儲存血液分離出來之血清的血清儲存槽,皆位在靠近中心The research and development of this disc-type biochemical sensing technology has been in foreign countries since about 1990, and has been making breakthroughs in the past one or two years. There have been products listed on the market. There are mainly three leading technology manufacturers: Gyros, Sweden, Tecan, Burstein, etc., among which patents on microfluidics are US4940527, US5006749, US5061381, US5122284, US5171695, US5173193, US5173262, US5186844, US5242606, US5242803, US5252294, US5275016, US53041, US5304348, US5304348 , US5409665, US5413732, US5426032, US5431303, US5432009, US5457063, US5472603, US5478750, US5496520 &gt; US5518930, US5590052, US5591643, US5599411, US5624597, US5639428, US 1943323248, US 1539323248, etc. Between 2001 and 2001, the patent content of US6302 1 34 was the most complete, and two specific embodiments for blood separation were provided in the text, as shown in Figures 1 to 11; the specific embodiments are shown in Figures 1 and 2. Blood From the operation status of the blood flow, both of which have a design in common, and that is separated from the serum storage tank storage of blood serum, are near the center position

第10頁 M273348Page 10 M273348

相當遠的位置,位在整個碟片的外圍部份,而此一碟片式 从流體系統的應用結構,受到其驅動力-離心力的作用方向 限制’基本上要將流體由離轉動中心點較遠的位置傳送至 $轉動中心點較近的位置是不太容易的事情,因此對於後 續其他血清處理程序的元件設計將會相當困難,此乃第一 項問題的所在。 第一項問題在於兩者皆使用蠟塊所形成的閥門,不僅增加 加工相當難度’而且需要適當的加熱機制,使得整個結構 設計變得更形複雜,成本較高。 第f項問題乃針對具體實施例二的狀況而言,由血液分離 2操作過程’可以看出它是在轉速高至將血液分離後,加 …、將峨塊溶解啟動閥門,將穩定槽5 〇 7内的流體送入血液分 =,50 9,提高其液面而將血清分離至儲存槽514,在這個 ,私中有一問題產生就是當穩定槽5 0 7内的流體要傳送至血 =刀離=5 0 9時,流道5 1 2 ·仍充滿空氣,而上下游皆有液體 ^在丄ί氣受到擠壓呈現不穩定的界面,雖然在高轉速f 二:f氣仍然會受到上方液體擠迫而進入血液分離槽5 0 9, 半:則相當難以控制對於未來生化檢測要求精準控制幾 十:法作自動化的程序控制,二則此一高轉速f會造 兀件設計困難。 ^ :m即針對上述的缺點進行&amp;㉟,第十三圖就是本 本概念設計圖,基材1〇為直徑12公分大類似光碟片 毛Γ ϊ目1配合創作中採用表面張力作為閥門的設計,避免 其材質為疏水性的材料,目前採用The far position is located in the outer part of the entire disc. The application structure of this disc type from the fluid system is limited by the direction of its driving force-centrifugal force. It is not easy to transfer the distant position to the position closer to the center of rotation, so it will be very difficult to design the components of other subsequent serum processing programs, which is the first problem. The first problem is that the valve formed by wax blocks is used for both, which not only increases the processing difficulty, but also requires a proper heating mechanism, which makes the overall structure design more complicated and costly. The f-th problem is for the situation of the specific embodiment 2. From the operation procedure of blood separation 2 ', it can be seen that after the speed is high enough to separate the blood, add ... The fluid in the 〇7 is sent to the blood, = 509, the serum level is raised and the serum is separated into the storage tank 514. Here, a problem arises when the fluid in the stabilization tank 507 is to be transferred to the blood = When the knife-off = 5 0 9, the flow path 5 1 2 is still full of air, and there are liquids in the upstream and downstream. ^ When the gas is squeezed, it shows an unstable interface, although at high speed f 2: f gas will still be affected. The upper liquid squeezes into the blood separation tank 509, half: it is quite difficult to control. For the future biochemical detection, precise control is required. Dozens of methods: automatic program control. Second, this high speed f makes it difficult to design the components. ^: m means &amp; ㉟ for the shortcomings mentioned above, the thirteenth figure is the conceptual design of the book, the base material 10 is 12 cm in diameter and is similar to the disc hair Γ ϊ Head 1 uses the surface tension as the valve design in the creation , To avoid its material being hydrophobic, currently used

M273348 四、創作說明(6) polydimethylsiloxane (PDMS),厚度為 〇.6 公釐;整個元 件結構1 0 0,配合人一滴血液的容量約略為2 5微升,又不希 望注入槽的直徑太大造成元件數目的限制,加工深度定為 400微米,直徑為8公釐;第十四〜十七圖為血液分離元件 的操作示意圖’圖中紅色部份代表血液及血球,皮膚色部 份代表血清,在此對其操作$式作一說明,首先將血液2 〇 微升利用精密手動注入器或自動化機械手臂經由上蓋的注 入孔輸送至注入槽,然後開始轉動,由於流道1 〇 8的尺寸蠻 大的’所以轉速不用太高約200〜300轉/分血液就會流入輔 助填充槽102,此一轉速並不薈造成閥門產丰作用,持 續數秒鐘後辅助填充槽102的液面超過溢流流道丨〇9的入口 處而流入血液分離槽103,最後形成如第十五圖所示;為產 生最大的分離效果,而且避免血液流入溢流流道丨丨〇,設計 時辅助填充槽及血液分離槽的尺寸必須經過計算及控制, 如此確保第十五圖的狀況產生,亦即輔助填充槽及血液分 離槽兩者的血液容量總和恰等於注入的血液量,液面幾乎 與溢流流道入口處相同。緊接著將轉動速率提高至血球血 清分離所需轉速約1 5 〇 〇轉/分,此時輔助填充槽及血液分離 槽兩者槽内的血液都會發生血球血清分離的現象,為確保 血液完全分離必須持續2〜3分鐘,然後再提高轉動速率至 20 0 0轉/分,使閥門105產生作用輔助填充槽槽内的液體流 入血液分離槽103,由於阻塊106的設計,所注入的液體= 會造成所分離血清血球的界面受到破壞,而且受到擠迫的 空氣會由阻塊上方的空隙流出造成壓力平衡,注入的液體M273348 Fourth, creation instructions (6) polydimethylsiloxane (PDMS), thickness is 0.6 mm; the entire element structure is 100, and the volume of a drop of blood is about 25 microliters, and the diameter of the injection groove is not too large. Due to the limitation of the number of components, the processing depth is set to 400 microns and the diameter is 8 mm; Figures 14 to 17 are schematic diagrams of the operation of blood separation components. Here, a description of its operation is given. First, 2 microliters of blood is transferred to the injection tank through the injection hole of the upper cover using a precision manual injector or an automated robotic arm, and then starts to rotate. Due to the size of the flow channel 108 It's quite big, so the speed does not need to be too high. About 200 ~ 300 rpm, blood will flow into the auxiliary filling tank 102. This speed does not cause the valve to produce abundant effects. After a few seconds, the liquid level of the auxiliary filling tank 102 exceeds The flow channel 丨 〇9 enters the blood separation tank 103, and finally forms as shown in Figure 15; in order to produce the maximum separation effect, and to prevent blood from flowing into the overflow channel 丨 丨The size of the auxiliary filling tank and the blood separation tank must be calculated and controlled during the design, so as to ensure that the situation in Figure 15 is generated, that is, the total blood volume of the auxiliary filling tank and the blood separation tank is equal to the blood volume injected. The liquid level is almost the same as at the inlet of the overflow channel. Immediately afterwards, the rotation rate is increased to about 15,000 revolutions per minute for blood cell serum separation. At this time, blood in both the auxiliary filling tank and the blood separation tank will undergo blood cell serum separation. In order to ensure complete blood separation Must continue for 2 ~ 3 minutes, and then increase the rotation rate to 2000 rpm to make the valve 105 work to assist the liquid in the filling tank to flow into the blood separation tank 103. Due to the design of the block 106, the injected liquid = Will cause the interface of the separated serum and blood cells to be damaged, and the compressed air will flow out from the gap above the block to cause pressure equilibrium. The injected liquid

第12頁 M273348 四、創作說明(7) 容量最佳狀況恰好使血液分離槽1 0 3内所分離#後的血清完全 由溢流流道11 0流出,但是因為人體的血清含量受到不同生 理狀況及體質的差異性,含量約佔血液容量的4 0〜6 0 %之 間,設計中為避免造成血球流入溢流流道11 0,輔助填充槽 102内的血液容量以血液分離槽103内血液容量(液面至溢 流流道入口)的4 0 %作為設計值;經過數十秒鐘後,流體 的分布情形如第十七圖所示。Page 12 M273348 IV. Creation instructions (7) The optimal capacity is exactly the condition that the serum separated # in the blood separation tank 103 is completely discharged from the overflow channel 110, but because the human serum content is affected by different physiological conditions And physical differences, the content accounts for about 40 ~ 60% of the blood volume. In order to avoid causing blood cells to flow into the overflow channel 110, the blood volume in the auxiliary filling tank 102 is based on the blood in the blood separation tank 103. 40% of the capacity (the liquid level to the inlet of the overflow channel) is taken as the design value; after several tens of seconds, the distribution of the fluid is shown in the seventeenth figure.

第13頁 M273348 圖式簡單說明 403 …血液分離區 4 0 4 …血液溢流槽 405…血清儲存槽 4 0 6 …毛細管流道 407 …毛細管40 6與408的銜接端 4 0 8 …毛細管流道 409 …空氣流道 4 1 0 …空氣流道中的銜接段可以避免液體流入 411 …微流道M273348 on page 13 Brief description of the drawing 403… blood separation area 4 0 4… blood overflow tank 405… serum storage tank 4 0 6… capillary channel 407… capillary 40 6 and 408 connecting end 4 0 8… capillary channel 409… air runner 4 1 0… the connecting section in the air runner prevents liquid from flowing into 411… micro runner

412 …閥門用蠟塊 413…閥門 5 0 1 …血液注入槽 5 0 2、5 0 3 …計量用毛細管流道 5 04 …溢流毛細管流道 5 0 5 …溢流槽 50 6 …毛細管的銜接段 50 7…穩定槽 5 0 8 …流道 5 0 9 …血液分離槽412… Wax block for valve 413… Valve 5 0 1… blood injection tank 5 0 2, 5 0 3… metering capillary channel 5 04… overflow capillary channel 5 0 5… overflow groove 50 6… capillary connection Segment 50 7… stabilizing groove 5 0 8… flow channel 5 0 9… blood separation groove

510 …毛細管道 511 …毛細管的銜接段 512 …微流道 514…儲存槽 515 …空氣流道510… capillary channel 511… capillary connecting section 512… microchannel 514… storage tank 515… air channel

第15頁 M273348 圖式簡單說明 517…儲存槽 518 、 519 …閥門 新型創作之圖號說明 10…PDMS材質直徑12公分大的碟片 100 …血液分離元件 1 0 1 …血液注入槽 102…輔助填充槽 103 …血液分離槽 1〇4 …血清儲存槽Page 15 M273348 Schematic description 517 ... Storage tanks 518, 519 ... Drawing number description of new valve creation 10 ... PDMS material disc 12 cm in diameter 100 ... Blood separation element 1 0 1 ... Blood injection tank 102 ... Supplementary filling Tank 103… blood separation tank 104… serum storage tank

105 …閥門 106 …阻塊 107 …空氣流道 108 …流道 10 9 …溢流道 110 …分離溢流道105… valve 106… block 107… air runner 108… runner 10 9… overflow channel 110… separation overflow channel

第16頁Page 16

Claims (1)

M273348 五、申請專利範圍 ! . —--- 1 · 一新型血液微分離元^件用於碟片式生化 ^ (a) —血液注入槽; 匕包括 (b) —辅助填充槽; (c) 一血液分離槽; (d) 血液分離槽内具有一卩且塊; (e) —閥門及兩個溢流流道。 2·如申請專利範圍第丨項所述之血液微分離元 ^ 分離槽中具有一阻塊,可以將已分離的血球主,匕在血液 充槽的液體相分隔,除可避免造成血清受到广=及來^填 以使來自填充槽的液體順利注入血液分離槽。‘卜還可 3.如申請專利範圍第1項所述之血液微分離元 槽内部的阻塊與其上方壁面間有一小距離=,血液分離 的寬度),作為空氣流道,平衡相對的流體壓I微米大小 4·如申請專利範圍第丨項所述之血液微分離。 槽與血液分離槽兩者的容積需經仔細 件,辅助填充 與血液分離槽兩•分別i溢流流道入口^的,助填充槽 血液注入量’而且輔助填充槽的容 :二積和等於 積量的40 %。 · 寺於血液分離槽容 5閃ΐ! ϊ專利範圍第1項所述之血液微分離元件,它且有-閥門而其啟動轉速值需高於 1干匕具有 6.如申請專利笳圊筐彳 、 求,月为離的轉速。 的材曾 Η 、所述之血液微分離元件,豆所使用 的材負可以是類似PDMS或剝田又η老m从 /、所仗用 乂利用不同處理後疏水性材質。M273348 5. Scope of patent application!. ----- 1 · A new type of blood micro-separation element is used for disc biochemical ^ (a) — blood injection tank; dagger includes (b) — auxiliary filling tank; (c) A blood separation tank; (d) a blood block in the blood separation tank; (e)-a valve and two overflow channels. 2. The blood micro-separation element described in item 丨 of the scope of the patent application ^ The separation tank has a block, which can separate the separated blood cells from the liquid phase in the blood-filling tank. In addition, it can prevent the serum from being damaged. = Time to fill so that the liquid from the filling tank is smoothly injected into the blood separation tank. 'Bu also 3. As described in the scope of the patent application, the internal block of the blood micro-separator cell has a small distance from the upper wall surface =, the width of blood separation), as an air flow channel, to balance the relative fluid pressure I micron size 4. Blood micro-separation as described in item 1 of the patent application scope. The volume of both the tank and the blood separation tank needs to be carefully examined. The auxiliary filling and the blood separation tank are two. • At the inlet of the overflow channel, the blood injection volume of the filling tank is aided. 40% of the volume. · Temple blood separation tank volume 5 flashes! ΪThe blood micro-separation element described in item 1 of the patent scope, which has a-valve and its startup speed value needs to be higher than 1 dry knife has 6. If you apply for a patent basket彳, ask, the speed of the moon is away. The material used in this article is the blood micro-separation element. The material used for the bean can be similar to PDMS or stripped from the ground and used for the purpose. 乂 Use different hydrophobic materials after processing. 第17頁 M273348Page 17 M273348 第18頁Page 18 第十四圖 第31頁Figure Fourteen Page 31
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