TWI812288B - Liquid handling module of PCR comprehensive diagnostic instrument - Google Patents

Abstract

A liquid processing module of a PCR comprehensive diagnostic instrument mainly includes a base and a sliding part, as well as a plurality of liquid-drawing structures. Among them, the liquid-drawing structures are arranged on the machine base in sequence for vertical and horizontal movement of different consumable cartridge trays of the PCR comprehensive diagnostic instrument, injecting PCR reagents into multiple sets of reaction test tubes, and then taking out the reaction test tubes. Cover, close the upper cover to the opening of the reaction test tube, and move the reaction test tube with the upper cover to the PCR detection module for detection. Moreover, these liquid-sucking structures can form left and right displacement intervals that can be adjusted to facilitate multiple groups of liquid-sucking structures to inject PCR reagents into multiple sets of reaction test tubes and move them to the PCR detection module for detection, thereby improving detection efficiency.

Description

Liquid handling module of PCR comprehensive diagnostic instrument

The invention relates to a liquid processing module of a PCR comprehensive diagnostic instrument, and in particular, to a PCR comprehensive device that provides multiple groups of liquid-drawing structures to inject PCR reagents into multiple groups of reaction test tubes and move them to the PCR detection module for detection to improve detection efficiency. The displacement structure of the liquid handling module of the diagnostic instrument.

Press; With the development of modern biomedical technology and clinical needs, many diagnostic methods require absolute quantification of target genes. For example, in the current technology to monitor the viral load and treatment course of infections such as the new coronavirus, in order to avoid mutual interference, qPCR technology must place the standard and the sample to be tested in different holes. Therefore, a considerable number of holes must be used. number, which may affect the experimental process and testing costs. Therefore, when using qPCR technology to perform absolute quantification of nucleic acid samples on test carriers with multiple reaction wells, how to improve the smoothness of the experiment and reduce the cost of testing is an important topic currently required for research.

The traditional method of nested PCR is to first place the reaction solution required for the primary PCR in a container. After the primary PCR thermal cycle reaction, or take out the primary PCR product and mix it with the secondary PCR reaction solution before performing the repeated PCR. PCR reaction, or directly add the secondary PCR reaction solution to the primary PCR reaction solution and mix before performing the secondary PCR reaction; the method of reverse transcription PCR is to first perform a reverse transcription reaction in a container, and then mix the mRNA or RNA with reverse transcription. The transcriptase converts it into CDNA, and then the CDNA is added to the PCR reaction solution to perform the PCR thermal cycle reaction. Whether it is nested PCR or reverse transcription PCR, there are actions of changing containers or adding transfers through the reaction solution during the process. These additional actions often increase the possibility of contamination of the reaction solution.

In another case, the applicant applied for a PCR comprehensive diagnostic instrument that can automatically perform the entire polymerase chain reaction process, thus saving time, reducing human errors and improving laboratory efficiency. However, the liquid handling module is single, which may affect the detection efficiency when the amount to be tested is large. If the number of liquid handling modules is increased, it is necessary to consider moving the reaction test tube from the reaction test tube carrier to the PCR detection module. For the arrangement and spacing of the groups, appropriately adjust the distance between the reaction test tubes. In view of this, after continuous research and experiments, the applicant came up with the idea of designing a liquid handling module displacement structure of a PCR comprehensive diagnostic instrument, which allows multiple groups of liquid-drawing structures to inject extraction liquid and PCR reagents into multiple groups of reaction test tubes and move them to the PCR detection module for detection to improve detection efficiency.

The main purpose of the present invention is to provide a liquid processing module of a PCR comprehensive diagnostic instrument, which allows multiple groups of liquid-drawing structures to inject PCR reagents into multiple groups of reaction test tubes, and appropriately adjusts the distance between the reaction test tubes to move to the PCR detection Module detection, thereby improving detection efficiency.

The liquid processing module of the aforementioned PCR comprehensive diagnostic instrument includes a base and a sliding member, as well as a plurality of liquid-drawing structures. Among them, the liquid-drawing structures are arranged on the machine base in sequence for vertical and horizontal movement of different consumable cartridge trays of the PCR comprehensive diagnostic instrument, injecting PCR reagents into multiple sets of reaction test tubes, and then taking out the reaction test tubes. Cover, close the upper cover to the opening of the reaction test tube, and move the reaction test tube with the upper cover to the PCR detection module for detection. Moreover, these liquid-sucking structures can form left and right displacement intervals that can be adjusted to facilitate multiple groups of liquid-sucking structures to inject PCR reagents into multiple sets of reaction test tubes and move them to the PCR detection module for detection, thereby improving detection efficiency.

The liquid processing module of the aforementioned PCR comprehensive diagnostic instrument includes a base and a sliding member. Among them, the machine base is provided with a driving member and a plurality of liquid sucking structures, and the liquid sucking structures are provided with horizontal protruding columns; the sliding member is pivotally connected to the driving member, and is provided with corresponding protruding columns. The guide grooves are tapered from top to bottom toward the center, so that the protruding columns of the liquid-sucking structures are embedded in the guide grooves. The driving member drives the sliding member to move up and down, so that the protrusions of the liquid-sucking structures move left and right along the guide grooves of the sliding member, so that the liquid-sucking structures can adjust the left and right displacement intervals. .

In the liquid processing module of the aforementioned PCR comprehensive diagnostic instrument, the liquid-drawing structures are provided with horizontal protruding columns; slide rails are provided on both sides of the machine base for two slide blocks to be embedded in the two slide rails. The rail drives the two slide blocks to move up and down through the driving member; the sliding member is fixed to the two slide blocks.

In the liquid processing module of the aforementioned PCR comprehensive diagnostic instrument, the sliding member is provided with lugs on both sides for fixing to the two sliding blocks.

Please refer to Figures 1 and 2 at the same time, together with Figures 3 and 4, which are three-dimensional views and structures of the PCR comprehensive diagnostic instrument of the present invention. As shown in the figure, the PCR comprehensive diagnostic instrument of the present invention is provided with a body 1, and the body 1 is provided with a touch display screen 11 and an entrance and exit 12. The body 1 is provided with a support frame 13 inside. The support frame 13 is provided with a reaction test tube carrier 2, a liquid processing module 3 and a PCR detection module 4. Among them, the reaction test tube carrier 2 includes two slide rails 21 and a platform 22 placed on the two slide rails 21. The two slide rails 21 are provided on the support frame 13, and guides are provided on both sides of the platform 22. groove 221, so that the guide groove 221 is embedded in the two slide rails 21. The support frame 13 is provided with a transmission member 23, and the transmission member 23 is connected to the platform 22 with a transmission belt 24, so that the transmission member 23 drives the platform 22 to move forward and backward, so that the platform 22 extends from the entrance 12, or Retract into body 1.

The platform 22 is provided with a hole panel area 25 for placing a consumable cassette tray 5, so that the consumable cassette tray 5 is placed with a reaction test tube 6 for containing the sample extract solution.

The liquid handling module 3 can move vertically and horizontally on different consumable cassette trays 5 to inject PCR reagents into the reaction test tube 6, and to set an upper cover to cover the opening of the reaction test tube 6. It can be moved to PCR detection module 4 for detection, thereby improving the efficiency of detection.

Please refer to FIG. 5 and FIG. 6 at the same time, which are three-dimensional exploded views of the liquid treatment module of the present invention. As shown in the figure, the liquid treatment module 3 of the present invention includes a base 31 and a sliding member 32. Among them, the machine base 31 is provided with a driving part 33 and a plurality of liquid sucking structures 34. The liquid sucking structures 34 are composed of a sleeve part 341, a piston shaft rod 342, and a driving device 343. Through the sleeve part 341 sets the needle tube, and drives the piston rod 342 and the set part 341 to move downward through the driving device 343, so as to inject the biological sample extract and PCR reagent to be detected into the reaction test tube 6, and withdraw the needle tube. and dismounting; and inserting an upper cover through the inserting part 341, and then moving downward through the piston shaft rod 342 to cover the opening of the reaction test tube 6 for moving to the PCR detection module 4 for detection.

The liquid-drawing structures 34 are provided with horizontal protruding columns 344; slide rails 311 are provided on both sides of the machine base 31 for two slide blocks 35 to be embedded in the two slide rails 311, and the two slide rails 311 are arranged on both sides of the base 31. The block 35 is pivotally connected to the aforementioned driving member 33, and the driving member 33 drives the two sliding blocks 35 to move up and down.

The sliding member 32 is provided with lugs 321 on both sides respectively for fixing to the two slide blocks 35. It is provided with a protruding post 344 corresponding to the liquid suction structure 34, and is tapered from top to bottom toward the center. The plurality of guide grooves 322 allows the protruding posts 344 of the liquid-sucking structures 34 to be embedded in the guide grooves 322 .

Please refer to Figure 7, which is a perspective view of the liquid treatment module of the present invention. Please cooperate with Figure 5 and Figure 6. Through the combination of the aforementioned components, the liquid treatment module 3 drives the sliding member 32 to move up and down through the driving member 33, so that the protruding pillars 344 of the liquid-sucking structures 34 The guide grooves 322 along the sliding member 32 move horizontally, thereby allowing the liquid-sucking structures 34 to adjust the left and right displacement intervals (the guide grooves 322 are tapered from top to bottom toward the center, so when moving downward When moving upward, the spacing between the liquid sucking structures 34 is smaller, and when moving upward, the spacing between the liquid sucking structures 34 is larger according to the gradual width of the guide groove 322), so as to facilitate multiple groups of liquid sucking structures 34 to inject PCR reagents into multiple sets of reactions. Test tube 6, then take the upper cover of the reaction test tube 6, close the upper cover to the opening of the reaction test tube 6, and move the covered reaction test tube 6 to the PCR detection module 4 for detection, thereby improving the detection efficiency. efficiency.

Please refer to Figure 8, which is an action state diagram of the present invention. Please cooperate with Figure 5 and Figure 6. As shown in the figures, when the present invention is in operation, the liquid processing module 3 not only injects PCR reagents into the reaction test tube 6 through the driving device 343, but also sets an upper cover on the reaction test tube. 6 for moving outside the PCR detection module 4. During the movement, the driving member 33 drives the sliding member 32 up and down. The protruding pillars 344 of the liquid-suction structures 34 are moved along the guide grooves 322 of the slider 32, so that the liquid-suction structures 34 can adjust the left and right displacement intervals, so as to facilitate the multiple groups of liquid-suction structures 34 to move together. PCR reagents are injected into multiple sets of reaction test tubes 6, then the upper caps of the reaction test tubes 6 are taken, the upper caps are closed on the openings of the reaction test tubes 6, and the reaction test tubes 6 with the upper caps are moved to the PCR detection module 4 for testing. detection, thereby improving detection efficiency. The detection result is displayed on the touch display 11 to improve detection efficiency. In this way, this PCR comprehensive diagnostic instrument can automatically perform the entire polymerase chain reaction process, which can save time, reduce human errors and improve laboratory efficiency, and can be used in medical laboratories for virus detection, precision medicine or forensics from a large number of samples. Forensics, etc., provide extraction and analysis of sample DNA or RNA.

The foregoing embodiments are only for illustrating the preferred embodiments of the present invention, rather than limiting the scope of the present invention. Slight modifications and changes may be made without losing the gist of the present invention and without departing from the spirit and scope of the present invention.

To sum up, the present invention uses a machine base provided with multiple sets of liquid suction structures, and a sliding member provided with a plurality of guide grooves coupled to the liquid suction structure to form a liquid processing module of a PCR comprehensive diagnostic instrument. It provides multiple groups of liquid draw structures to inject PCR reagents into multiple groups of reaction test tubes, and appropriately adjusts the distance between the reaction test tubes and moves them to the PCR detection module for detection, thereby improving detection efficiency. It is a practical design and is truly a novel creation. I submit a patent application in accordance with the law and pray that the Bureau will review it and grant the patent as soon as possible.

1: Body

11:Touch display

12: Entrance and exit

13:bearing frame

2: Reaction test tube carrier

21:Slide rail

22:Platform

221:Guide groove

23: Transmission parts

24:Transmission belt

25: Hole panel

3: Liquid handling module

31: Machine base

311:Slide rail

32:Sliding parts

321:Lug

322:Guide groove

33:Driving parts

34:Liquid-sucking structure

341: Setup Department

342:Piston shaft rod

343:Driving device

344:Protruding pillar

35:Slider

4: PCR detection module

5: Consumable cassette tray

6: Reaction test tube

Figure 1 is a three-dimensional view of the PCR comprehensive diagnostic instrument of the present invention. Figure 2, Figure 3 and Figure 4 are structural diagrams of the PCR comprehensive diagnostic instrument of the present invention. Figures 5 and 6 are exploded three-dimensional views of the liquid treatment module of the present invention. Figure 7 is a perspective view of the liquid treatment module of the present invention. Figure 8 is an operation state diagram of the present invention.

3: Liquid handling module

31: Machine base

33:Driving parts

34:Liquid-sucking structure

341: Setup Department

342:Piston shaft rod

343:Driving device

35:Slider

Claims (4)

A liquid processing module of a PCR comprehensive diagnostic instrument, which includes a base and a plurality of liquid sucking structures, wherein the liquid sucking structures are arranged on the base in sequence for longitudinal and transverse movement of the PCR comprehensive diagnostic instrument. Use different consumable cassette trays to inject PCR reagents into multiple sets of reaction test tubes, then take the upper caps of the reaction test tubes, close the upper caps to the openings of the reaction test tubes, and place the caps that have been covered with the reaction test tubes. The reaction test tubes are moved to the PCR detection module for detection; the liquid-sucking structures can be adjusted along the left and right displacement intervals of the machine base to facilitate the injection of PCR reagents into the reaction test tubes by the liquid-sucking structures , and move it to the PCR detection module for detection, thereby improving the efficiency of detection. A liquid processing module of a PCR comprehensive diagnostic instrument. The liquid processing module includes a base and a sliding part. The base is provided with a driving part and a plurality of liquid-drawing structures. The liquid-drawing structures are combined In the sliding part; the sliding part is pivotally connected to the driving part; the driving part drives the liquid-absorbing structures to form left and right displacement interval adjustment. The liquid processing module of the PCR comprehensive diagnostic instrument as described in claim 2, wherein the liquid suction structures are provided with horizontal protruding columns; slide rails are provided on both sides of the base for two slide blocks. Embedded in the two slide rails, the driving member drives the two slide blocks to move upward and downward; the sliding member is fixed to the two slide blocks, and is provided with protruding pillars corresponding to the liquid suction structures, and There are a plurality of guide grooves tapering from top to bottom toward the center, so that the protruding columns of the liquid-sucking structure are embedded in the guide grooves; so that the driving part drives the sliding part to move up and down, so that the sliding part moves up and down. The protrusions of the liquid-sucking structures move along the guide grooves of the sliding member, thereby enabling the left and right displacement intervals of the liquid-sucking structures to be adjusted. The liquid processing module of the PCR comprehensive diagnostic instrument as described in claim 3, wherein the sliding member is provided with lugs on both sides for fixing to the two slide blocks.