TWI812118B - Miniature multiplex testing chip, multiplex testing equipment and testing method thereof - Google Patents

Abstract

According to the present disclosure, a miniature multiplex testing chip includes a testing portion and an identifying portion. The testing portion includes a calibrating region, a retention region and a plurality of testing regions. The calibrating region is disposed on a surface of the testing portion. The retention region is disposed around the calibrating region and communicated with outside. The testing regions are disposed around the retention region sequentially, and each of the testing regions is communicated with the retention region or outside. The identifying portion is connected to the testing portion. The identifying portion includes an identifying mark, so as for the user to identify the type of test. The calibrating region, the retention region and the testing regions are arranged in a concentric manner. In this regard, multiple tests can be performed on the miniature multiplex testing chip of the present disclosure simultaneously. Thus, the testing time can be shortened and the testing processes can be simplified significantly, and the required quantity of sample for the tests can also be reduced.

Description

Miniature multiple detection chip, multiple detection equipment and detection method

The present invention relates to a micro-detection chip, detection equipment and detection methods, and in particular to a micro-multiple detection chip, multiple detection equipment and detection methods that can perform several detection items simultaneously.

With the advancement of testing science, in addition to testing through professional laboratory experimental procedures, many convenient-to-use test strips have also been developed. Conventional test strips usually include a detection tank containing specific reagents. The type of reagent depends on the target substance to be detected. When using conventional detection test strips, you only need to add the specimen into the detection tank, and after the reaction between the specimen and the reagent is completed, observe or measure the property changes of the reagent to obtain the detection result.

However, the conventional test strips can only detect one target substance at a time. If you want to detect multiple target substances, you need to prepare test strips corresponding to various target substances, and the required amount of specimens also increases significantly. Not only does it require The testing time is long, the testing process is also more cumbersome, and collecting a large number of specimens is more likely to cause discomfort to the subjects.

In view of this, how to efficiently detect multiple target substances to simplify the detection process and reduce the discomfort of the subjects is still a problem to be solved.

One object of the present invention is to provide a miniature multiplex detection chip that can accommodate multiple detection reagents at the same time and can perform multiple detections at the same time.

One aspect of the present invention provides a micro multiple detection chip, which includes a detection part and an identification part. The detection part includes a calibration area, a storage area and a plurality of detection areas. The calibration area is located on the surface of the detection part. The storage area is surrounding the calibration area and connected to the outside world. The detection areas are arranged in sequence around the storage area. And each detection area is connected to the storage area or the outside world respectively. The identification part is connected to the detection part, and the identification part has an identification mark for the user to identify the content of the detection. Among them, the calibration area, storage area and detection area are arranged concentrically.

According to this, the micro multiple detection chip of the present invention has a calibration area, a storage area and a detection area arranged sequentially from the inside out. When performing detection, it is only necessary to place different detection reagents in each detection area and place the specimen By adding it to the storage area and/or testing area, multiple tests can be performed at the same time, thereby significantly reducing the testing time and testing process, and also reducing the demand for specimens.

According to the aforementioned micro multiple detection chip, the storage area can be connected to the outside through the opening, and the detection part can further include a sealing member, which can removably seal the opening, and the sealing member can be made of a dissolvable material.

According to the aforementioned micro multi-detection chip, the detection area may not be connected to the outside world. When the sealing member closes the opening, the pressure in the storage area and the detection area may be less than the outside world, and each detection area may include a micro-channel to connect the storage area. .

Another aspect of the present invention provides a multiplex detection device, which includes the aforementioned micro multiplex detection chip and a chip analysis device. The chip analysis device carries the micro multiplex detection chip and is used to measure and analyze the detection results of the micro multiplex detection chip. And includes processor, camera module and output module. The photography module is coupled to the processor. The photography module is used to capture images of the micro multi-detection chip and transmit the images to the processor for analysis. The output module is coupled to the processor, and the output module is used to output detection results analyzed by the processor.

According to the aforementioned multiple detection equipment, the chip analysis device may further include an electrophysical module. The electrophysical module is coupled to the processor. The electrophysical module may include an electrophysical sensing element. The electrophysical sensing element may be disposed on a micro multiplexer. In the storage area and/or detection area of the detection chip, the electrophysical module can be used to detect the physical or chemical properties in the storage area and/or the detection area, and transmit the physical or chemical properties to the processor for processing. analyze.

According to the aforementioned multiple detection equipment, the chip analysis device can further include an embedded module. The embedded module is coupled to the processor. The embedded module can be used to control the chip analysis device to perform image shooting, data analysis and processing on the micro multi-detection chip. Result output.

Another aspect of the present invention provides a multiplex detection method, which includes the following steps: providing the aforementioned multiplex detection equipment, performing a detection step, performing an analysis step, and performing an output step, wherein each detection area of the micro multiplex detection chip is respectively accommodated Detection reagents. In the detection step, the specimen is dropped into the storage area and/or the detection area of the micro multiplex detection chip, so that the specimen reacts with the detection reagents in each detection area. In the analysis step, the photography module of the chip analysis device is used to photograph the micro multi-detection chip to obtain the image, and the image is sent to the processor for analysis to obtain the detection result. In the output step, the detection results are output using the output module of the chip analysis device.

According to the aforementioned multiplex detection method, the detection area of the micro multiplex detection chip may not be connected to the outside world, each detection area may include a microfluidic channel to connect to the storage area, and the detection reagents may be accommodated in the microfluidic channel of each detection area.

According to the aforementioned multiple detection method, the chip analysis device of the multiple detection equipment may further include an electrophysical module, and the electrophysical module is coupled to the processor. The electrophysical module may include an electrophysical sensing element, and the electrophysical sensing element may It is arranged in the storage area and/or detection area of the micro multiplex detection chip. When performing the analysis step, the electrophysical and chemical module can detect the micro multiple detection chip to measure the physical or chemical properties in the storage area and/or the detection area, and transmit the physical or chemical properties to the processor. Perform analysis.

According to the aforementioned multiple detection method, the analysis step may further include the following steps: a detection content confirmation step and a colorimetric step. In the detection content confirmation step, the processor can be used to read the identification marks in the image and load the analysis process according to the identification marks. In the colorimetric step, the processor can be used to compare the color difference between the calibration area and the detection reagent after reaction, and the color difference can be analyzed according to the analysis process to obtain the detection result.

Various embodiments of the invention are discussed in greater detail below. However, the embodiments are applicable to various inventive concepts and may be embodied in various specific scopes. The specific embodiments are provided for illustrative purposes only and do not limit the scope of the disclosure.

Please refer to Figure 1. Figure 1 is a schematic top view of a miniature multiplex detection chip 100 according to an embodiment of the present invention. The micro multiple detection chip 100 includes a detection part 110 and an identification part 120, and the identification part 120 is connected to the detection part 110.

In detail, the detection part 110 includes a calibration area 111 , and the calibration area 111 is provided on the surface of the detection part 110 . The calibration area 111 is used for comparison after the detection. For example, if the detection reagent used in the micro multiplex detection chip 100 is a color-changing reagent (such as litmus reagent or acetone reagent), the calibration area 111 can be white. Or the original color of the detection reagent can be obtained by comparing the calibration area 111 with the detection reagent after the reaction, and the degree of color change of the detection reagent can be known, and the detection result can be deduced based on the above color change.

The detection part 110 includes a storage area 112. The storage area 112 is located around the calibration area 111 and is connected to the outside world. The storage area 112 is used to store the specimen to facilitate comparison or observation of the condition of the specimen. The storage area 112 can be connected to the outside through the opening A, and the detection part 110 can further include a closing member (not shown), and the closing member can removably close the opening A to prevent dust or impurities from entering the storage area 112 during storage. Test results. It is worth mentioning that the structure of the closure can be a physically removable structure, such as a sealing film or a pull-tab closure, or the closure can be made of a dissolvable material and can be made into a film. Closing the opening A, when the sample containing the solvent comes into contact with the sealing member, it can dissolve the sealing member and enter the storage area 112 .

In addition, the storage area 112 can additionally contain adsorption materials corresponding to the specimen and the inspection method for adsorbing the specimen. The adsorbent material can be tissue paper, sponge or fluff pulp, or sodium polyacrylate, polyacrylamide, or ethylene-maleic anhydride copolymer. , cross-linked sodium carboxymethyl cellulose or polyvinyl alcohol and other adsorbent materials that do not affect the inspection method and specimen.

The detection part 110 includes a plurality of detection areas 113. The detection areas 113 are arranged in order around the storage area 112, and each detection area 113 is connected to the storage area 112 or the outside world. If each detection area 113 is connected to the storage area 112, proceed During detection, the specimen can be added to the storage area 112 so that the specimen flows into each detection area 113 on its own. In addition, the calibration area 111, the storage area 112 and the detection area 113 are arranged concentrically, so that the specimen is evenly dispersed in the storage area 112 and enters each detection area 113 evenly, thereby reducing detection errors. Each detection area 113 can also accommodate adsorption materials respectively, the details of which are the same as the adsorption materials in the aforementioned storage area 112, and will not be described again here.

The identification part 120 has an identification mark 121 for the user to identify the detected content. The identification mark 121 can be a picture, text, one-dimensional barcode, two-dimensional barcode (such as QR code) or radio frequency tag (such as RFID tag). However, this Inventions are not limited to this.

Please refer to Figures 2 and 3. Figure 2 is a schematic top view of a micro multiplex detection chip 200 according to another embodiment of the present invention. Figure 3 is a schematic top view of the micro multiplex detection chip 200 in Figure 2 along 3- 3 line section schematic diagram. The structure of the micro multiple detection chip 200 is roughly the same as that of the micro multiple detection chip 100 of the previous embodiment. The difference is that the detection area 213 is not connected to the outside world, and when the sealing member closes the opening A' of the storage area 212, the storage area 213 is not connected to the outside world. The pressure in the retention area 212 and the detection area 213 can be smaller than the outside world, and each detection area 213 can respectively include a micro-channel C to connect the retention area 212. By creating a negative pressure environment and establishing a microfluidic channel C, the specimen can be helped to smoothly enter the detection area 213 from the storage area 212 without backflow, thereby avoiding cross-contamination of specimens or test reagents in different detection areas 213. .

Please refer to Figure 4. Figure 4 is a schematic structural diagram of a multiplex detection device 300 in another aspect of the present invention. The multiplex detection equipment 300 includes the aforementioned micro multiplex detection chip 100 and the chip analysis device 310. The chip analysis device 310 carries the micro multiplex detection chip 100 and can measure and analyze the detection results of the micro multiplex detection chip 100. It includes a processor 311, The photography module 312 and the output module 313 are coupled to the processor 311 respectively.

The photography module 312 is used to capture images of the miniature multi-detection chip 100 and transmit the images to the processor 311 for analysis. The detailed analysis process will be introduced in subsequent paragraphs and will not be described again here.

The output module 313 is used to output the detection results analyzed by the processor 311. Therefore, the output module 313 can be a display or a printer, etc., and the output module 313 can also be a mobile device or a wireless device, and transmits data via Bluetooth or The Internet and other signals are connected to the processor 311, so that the user can receive the test results remotely through a mobile phone or other device, thereby improving the convenience of use.

The chip analysis device 310 may further include an electrophysical module 314. The electrophysical module 314 is coupled to the processor 311. The electrochemical module 314 may include an electrophysical sensing element 315, which may be disposed on the reservoir of the micro multi-detection chip 100. area 112 and/or detection area 113. The electrophysical module 314 can be used to detect physical properties or chemical properties in the storage area 112 and/or the detection area 113, and transmit the properties to the processor 311 for analysis.

The chip analysis device 310 may further include an embedded module 316. The embedded module 316 is coupled to the processor 311. The embedded module 316 may be used to control the chip analysis device 310 to perform image capture, data analysis and results on the micro multi-detection chip 100. Output, that is, the user can indirectly control the calculation and operation of the processor 311, the photography module 312, the output module 313 and the electrochemical module 314 by controlling the embedded module 316.

Please refer to Figure 5. Figure 5 is a step flow chart of a multiplex detection method 400 of another aspect of the present invention. The multiplex detection method 400 includes step 410, step 420, step 430 and step 440.

Step 410 is to provide the aforementioned multiplex detection equipment, in which detection reagents are respectively stored in each detection area of the micro multiplex detection chip. The types of detection reagents in each detection area can be different from each other, so that multiple detections can be performed simultaneously.

Step 420 is a detection step, which involves dropping the specimen into the storage area and/or detection area of the micro multiplex detection chip, allowing the specimen to react with the detection reagents in each detection area. If the detection area is not connected to the outside world, the specimen must be dropped into the storage area, and the reaction must be carried out after the specimen flows into the detection area. Therefore, the detection reagent can be accommodated in the microfluidic channel of each detection area. When the specimen flows through the microfluidic After the flow channel, the reaction is also completed at the same time, so the detection time can be effectively shortened.

Step 430 is an analysis step, which uses the photography module of the chip analysis device to photograph the micro multi-detection chip to obtain the image, and transmit the image to the processor for analysis to obtain the detection result. The image can be a static image or a dynamic image. For example, if the analysis method is to compare the color of the detection reagent before and after the reaction, the image can be a static image before and after the reaction. If the analysis method is to observe the detection at different time points If the reagent changes, the image can be a dynamic image of the reaction process.

In addition, the chip analysis device of the multi-detection equipment may further include an electrophysical module, the details of which are the same as the aforementioned electrophysical module 314, and will not be described again here. In the analysis step, the electrophysical and chemical module can detect the micro multiple detection chip to measure the physical or chemical properties in the storage area and/or the detection area, such as changes in conductivity, and transmit the properties to the processing The processor performs analysis, thereby providing the processor with different detection information, which can be combined with the aforementioned images for comprehensive analysis, helping to improve the accuracy of the detection results.

Please refer to Figure 6 as well. Figure 6 is a detailed flow chart of step 430 in the multiplex detection method 400 in Figure 5. The analysis step 430 may further include step 431 and step 432.

Step 431 is a test content confirmation step. The processor is used to read the identification marks in the image and load the analysis process according to the identification marks. That is, the processor has preset different analysis processes for different test items. The processor can determine the current detection item through the identification mark, and load the corresponding analysis process for subsequent analysis.

Step 432 is a colorimetric step. The processor is used to compare the color difference between the calibration area and the detected reagent after reaction, and the color difference is analyzed according to the analysis process to obtain the detection result. For example, if the processor determines in step 431 that the detection reagent used is litmus reagent, the processor loads the analysis process corresponding to the litmus reagent, and then calculates the result by comparing the color of the calibration area and the detection reagent in the image. Detect the three primary color light values of the reagent, and read the corresponding pH based on the three primary color light values as the test result.

Step 440 is an output step, which uses the output module of the chip analysis device to output the detection results. The details of the output module have been explained in the previous paragraphs and will not be repeated here.

To sum up, the micro multiple detection chip of the present invention has a calibration area, a storage area and a detection area arranged sequentially from the inside out. When performing detection, only different detection reagents need to be placed in each detection area, and By adding specimens to the storage area and/or testing area, multiple tests can be performed at the same time. This can significantly reduce the testing time and process, as well as reduce the demand for specimens.

Although the present invention has been disclosed above through embodiments, they are not intended to limit the present invention. Anyone skilled in the art can make various modifications and modifications without departing from the spirit and scope of the present invention. Therefore, the protection of the present invention is The scope shall be determined by the appended patent application scope.

100,200:Micro multiple detection chip 110:Testing Department 111:Calibration area 112,212:Retention area 113,213:Detection area 120:Identification Department 121:Identification mark 300:Multiple detection equipment 310:wafer analysis device 311: Processor 312:Photography module 313:Output module 314: Electrophysical module 315:Electrophysical sensing element 316: Embedded module 400:Multiple detection methods 410,420,430,431,432,440: Steps A,A': open C: Microfluidic channel

In order to make the above and other objects, features, advantages and embodiments of the present invention more apparent and understandable, the accompanying drawings are described as follows: Figure 1 is a schematic top view of a miniature multiplex detection chip according to an embodiment of the present invention; Figure 2 is a schematic top view of a miniature multiplex detection chip according to another embodiment of the present invention; Figure 3 is a schematic cross-sectional view of the micro multiple detection chip along line 3-3 in Figure 2; Figure 4 is a schematic structural diagram of a multiple detection device of another aspect of the present invention; Figure 5 is a step flow chart of another aspect of the multiplex detection method of the present invention; and Figure 6 is a detailed flow chart of step 430 in the multiplex detection method of Figure 5.

100:Micro multiple detection chip

110:Testing Department

111:Calibration area

112:Retention area

113:Detection area

120:Identification Department

121:Identification mark

A:Open your mouth

Claims (9)

A miniature multiple detection chip, including: a detection part, including: a calibration area, located on a surface of the detection part; a storage area, surrounding the calibration area, the storage area is connected to the outside world and passes through a The opening is connected to the outside; a closure that removably closes the opening, and the closure is made of a dissolvable material; and a plurality of detection areas arranged in sequence in the retention area around, and each detection area is connected to the storage area or the outside world respectively; and an identification part is connected to the detection part, and the identification part has an identification mark for a user to identify the content of the detection; wherein, the calibration area , the storage area and the detection areas are arranged concentrically. The micro multi-detection chip as described in claim 1, wherein the detection areas are not connected to the outside world. When the closing member closes the opening, the pressure in the storage area and the detection areas is less than the outside world, and each detection area Each contains a microfluidic channel to connect the retention area. A multiplex detection device, including: a micro multiplex detection chip as described in claim 1; and a chip analysis device carrying the micro multiplex detection chip, and the chip analysis device is used to measure and analyze the micro multiplex detection chip A test result of the wafer, the wafer analysis device includes: a processor; a photography module coupled to the processor, the photography module is used to capture an image of the miniature multi-detection chip and transmit the image to the processor for analysis; and an output module coupled Connected to the processor, the output module is used to output the detection result analyzed by the processor. The multiple detection equipment of claim 3, wherein the chip analysis device further includes: an electrophysical module coupled to the processor, the electrophysical module includes at least one electrophysical sensing element, and the electrophysical sensing element The element is arranged in the storage area and/or the detection areas of the micro multiple detection chip; wherein, the electrochemical module is used to detect a physical property or a physical property in the storage area and/or the detection areas. chemical property, and transmit the physical property or the chemical property to the processor for analysis. The multiplex detection equipment of claim 3, wherein the chip analysis device further includes an embedded module coupled to the processor, and the embedded module is used to control the chip analysis device to image the micro multiplex detection chip Photography, data analysis and result output. A multiplex detection method, including: providing the multiplex detection equipment as described in claim 3, wherein each detection area of the micro multiplex detection chip contains a detection reagent; To perform a detection step, a sample is dropped into the storage area and/or the detection areas of the micro multiplex detection chip, so that the specimen reacts with the detection reagents in each detection area; The analysis step is to use the photography module of the chip analysis device to photograph the micro multi-detection chip to obtain the image, and transmit the image to the processor for analysis to obtain the detection result; and perform an output. In the step, the output module of the chip analysis device is used to output the detection result. The multiplex detection method as described in claim 6, wherein the detection areas of the micro multiplex detection chip are not connected to the outside world, each detection area includes a microfluidic channel to connect to the storage area, and the detection reagent is accommodated in the microfluidic channel of each detection area. The multiple detection method as described in claim 6, wherein the chip analysis device of the multiple detection equipment further includes: an electrophysical module coupled to the processor, the electrophysical module including at least one electrophysical sensing element, The electrophysical sensing element is disposed in the storage area and/or the detection areas of the micro multiple detection chip; wherein, when performing the analysis step, the electrophysical module detects the micro multiple detection chip to detect A physical property or a chemical property in the storage area and/or the detection areas is measured, and the physical property or the chemical property is sent to the processor for analysis. The multiple detection method as described in claim 6, wherein the analysis step further includes: performing a detection content confirmation step, using the processor to read the identification mark in the image, and loading an analysis process according to the identification mark ; and perform a colorimetric step, using the processor to compare a color difference between the calibration area and the detection reagent after reaction, and analyzing the color difference according to the analysis process to obtain the detection result.

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