TWI811340B - Beverage, packaged beverage and method for inhibiting coagulation of fungus or treated fungus - Google Patents
Beverage, packaged beverage and method for inhibiting coagulation of fungus or treated fungus Download PDFInfo
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- TWI811340B TWI811340B TW108113580A TW108113580A TWI811340B TW I811340 B TWI811340 B TW I811340B TW 108113580 A TW108113580 A TW 108113580A TW 108113580 A TW108113580 A TW 108113580A TW I811340 B TWI811340 B TW I811340B
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- VQHSOMBJVWLPSR-JVCRWLNRSA-N lactitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O VQHSOMBJVWLPSR-JVCRWLNRSA-N 0.000 description 1
- 229960003451 lactitol Drugs 0.000 description 1
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- 239000004033 plastic Substances 0.000 description 1
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- 229920000642 polymer Polymers 0.000 description 1
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- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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- 239000001509 sodium citrate Substances 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
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- 235000019408 sucralose Nutrition 0.000 description 1
- BAQAVOSOZGMPRM-QBMZZYIRSA-N sucralose Chemical compound O[C@@H]1[C@@H](O)[C@@H](Cl)[C@@H](CO)O[C@@H]1O[C@@]1(CCl)[C@@H](O)[C@H](O)[C@@H](CCl)O1 BAQAVOSOZGMPRM-QBMZZYIRSA-N 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- 235000010491 tara gum Nutrition 0.000 description 1
- 239000000213 tara gum Substances 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
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- 229940046008 vitamin d Drugs 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/60—Drinks from legumes, e.g. lupine drinks
- A23L11/65—Soy drinks
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Non-Alcoholic Beverages (AREA)
- General Preparation And Processing Of Foods (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
Abstract
本發明之飲料不含源自乳之蛋白質,且含有菌體及/或菌體處理物、及大豆多醣類,並滿足以下之式1~3;式1:0.001質量%≦(菌體及/或菌體處理物之含量)(質量%)≦0.22質量%;式2:0.0001質量%≦大豆多醣類含量(質量%)≦1質量%;式3:0.1≦{大豆多醣類含量(質量%)/(菌體及/或菌體處理物之含量)(質量%)}。The beverage of the present invention does not contain protein derived from milk, contains bacterial cells and/or bacterial cell processed materials, and soybean polysaccharides, and satisfies the following formulas 1 to 3; Formula 1: 0.001 mass %≦ (bacterial cells and / or the content of the bacterial cell treatment) (mass %) ≦ 0.22 mass %; Formula 2: 0.0001 mass % ≦ soybean polysaccharide content (mass %) ≦ 1 mass %; Formula 3: 0.1 ≦ {soybean polysaccharide content (mass %)/(content of bacterial cells and/or bacterial cell treated materials) (mass %)}.
Description
本發明係關於一種飲料、容器裝飲料、及抑制菌體或菌體處理物之凝聚之方法。The present invention relates to a beverage, a container-packed beverage, and a method for inhibiting the aggregation of bacterial cells or bacterial cell treated materials.
習知,就可口性、外觀、保存性等觀點而言,於飲料中摻合有各種成分。例如於專利文獻1中,揭示有於包含醱酵乳之飲料中,使用大豆多醣類及水不溶性之醱酵纖維素粒子以抑制乳蛋白之沈澱。It is known that various ingredients are blended into beverages from the viewpoints of palatability, appearance, shelf life, etc. For example, Patent Document 1 discloses the use of soybean polysaccharides and water-insoluble fermented cellulose particles in a beverage containing fermented milk to suppress the precipitation of milk protein.
又,另一方面,以近年來之健康意向之高漲等為背景,作為功能性飲料,摻合有乳酸菌者受到關注。雖有包含乳酸菌之飲料,但於該含有乳酸菌之飲料中,已知乳酸菌粉末之凝聚。因此,例如於專利文獻2中,揭示有藉由含有天然結冷膠及水溶性鈣鹽抑制pH,而使乳酸菌粉末等不溶性固形物成分進行分散之技術。 先前技術文獻 專利文獻On the other hand, against the backdrop of rising health concerns in recent years, functional beverages incorporating lactic acid bacteria are attracting attention. There are beverages containing lactic acid bacteria, but in the beverages containing lactic acid bacteria, aggregation of lactic acid bacteria powder is known. Therefore, for example, Patent Document 2 discloses a technology in which insoluble solid components such as lactic acid bacteria powder are dispersed by suppressing pH by containing natural gellan gum and water-soluble calcium salts. Prior technical literature patent documents
專利文獻1:日本特開2014-19號公報 專利文獻2:日本特開2007-325506號公報Patent Document 1: Japanese Patent Application Publication No. 2014-19 Patent Document 2: Japanese Patent Application Publication No. 2007-325506
[發明所欲解決之課題][Problem to be solved by the invention]
然而,專利文獻1中所記載之技術係併用不溶性之醱酵纖維素粒子與大豆多醣類而抑制因包含於醱酵乳中之蛋白質之凝聚等所產生的沈澱。又,專利文獻2中所記載之技術需要天然結冷膠(native gellan gum)等特定之高分子多醣類。天然結冷膠有因酸而分解之傾向,故而可使用之酸性成分受到限定等,於設計之自由度方面存在改善之餘地。However, the technology described in Patent Document 1 uses insoluble fermented cellulose particles and soybean polysaccharides in combination to suppress precipitation due to aggregation of proteins contained in fermented milk. In addition, the technology described in Patent Document 2 requires specific polymer polysaccharides such as native gellan gum. Natural gellan gum has a tendency to decompose due to acid, so the acidic ingredients that can be used are limited, and there is room for improvement in terms of design freedom.
本發明者為了含有乳酸菌等菌體或菌體處理物且抑制菌體或菌體處理物之絮狀凝聚物,而潛心進行了研究。結果發現,存在藉由將菌體或菌體處理物與大豆多醣類以規定之比率摻合而獲得良好之抑制凝聚之效果的條件,從而完成了本發明。尤其對於不含源自乳之蛋白質之飲料而言有效。 [解決課題之技術手段]The present inventors conducted intensive research in order to contain bacterial cells or treated bacterial cells such as lactic acid bacteria and suppress floc aggregates of the bacterial cells or treated bacterial cells. As a result, they found that there are conditions under which a good aggregation-inhibiting effect can be obtained by blending bacterial cells or bacterial cell processed materials with soybean polysaccharides at a predetermined ratio, and the present invention was completed. It is especially effective for beverages that do not contain milk-derived protein. [Technical means to solve the problem]
根據本發明, 提供一種飲料,係不含源自乳之蛋白質,且 含有菌體及/或菌體處理物、及大豆多醣類,並滿足以下之式1~3; 式1:0.001質量%≦(菌體及/或菌體處理物之含量)(質量%)≦0.22質量%; 式2:0.0001質量%≦大豆多醣類含量(質量%)≦1質量%; 式3:0.1≦{大豆多醣類含量(質量%)/(菌體及/或菌體處理物之含量)(質量%)}。According to the present invention, Provide a beverage that does not contain protein derived from milk and Contains bacterial cells and/or bacterial cell processed materials, and soybean polysaccharides, and satisfies the following formulas 1 to 3; Formula 1: 0.001 mass % ≦ (content of bacterial cells and/or bacterial cell treated materials) (mass %) ≦ 0.22 mass %; Formula 2: 0.0001 mass% ≦ soybean polysaccharide content (mass %) ≦ 1 mass%; Formula 3: 0.1≦{Soybean polysaccharide content (mass %)/(content of bacterial cells and/or bacterial cell treatments) (mass %)}.
又,根據本發明, 提供一種飲料,其不含源自乳之蛋白質,且 含有菌體及/或菌體處理物、及大豆多醣類,並滿足以下之式4~6, 式4:0.22質量%<(菌體及/或菌體處理物之含量)(質量%)≦0.35質量%; 式5:0.044質量%<大豆多醣類含量(質量%)≦1質量%; 式6:0.2≦{大豆多醣類含量(質量%)/(菌體及/或菌體處理物之含量)(質量%)}。Furthermore, according to the present invention, Provide a beverage that does not contain protein derived from milk, and Contains bacterial cells and/or bacterial cell processed materials, and soybean polysaccharides, and satisfies the following formulas 4 to 6, Formula 4: 0.22 mass % < (content of bacterial cells and/or bacterial cell treated materials) (mass %) ≦ 0.35 mass %; Formula 5: 0.044 mass % < soybean polysaccharide content (mass %) ≦ 1 mass %; Formula 6: 0.2≦{Soybean polysaccharide content (mass %)/(content of bacterial cells and/or bacterial cell treatments) (mass %)}.
根據本發明,提供一種容器裝飲料,其係將上述飲料填充至容器而成。According to the present invention, there is provided a container-packed beverage in which the above-mentioned beverage is filled into a container.
又,根據本發明, 提供一種抑制菌體或菌體處理物之凝聚之方法,其係於含有菌體及/或菌體處理物、及大豆多醣類之飲料中,滿足以下之式8~10, 式8:0.001質量%≦(菌體及/或菌體處理物之含量)(質量%)≦0.22質量%; 式9:0.0001質量%≦大豆多醣類含量(質量%)≦1質量%; 式10:0.1≦{大豆多醣類含量(質量%)/(菌體及/或菌體處理物之含量)(質量%)}。Furthermore, according to the present invention, Provided is a method for inhibiting the aggregation of bacterial cells or bacterial cell processed substances in a beverage containing bacterial cells and/or bacterial cell processed substances, and soybean polysaccharides, and satisfying the following formulas 8 to 10, Formula 8: 0.001 mass % ≦ (content of bacterial cells and/or bacterial cell treated materials) (mass %) ≦ 0.22 mass %; Formula 9: 0.0001 mass % ≦ soybean polysaccharide content (mass %) ≦ 1 mass %; Formula 10: 0.1 ≦ {soybean polysaccharide content (mass %)/(content of bacterial cells and/or bacterial cell treatments) (mass %)}.
又,根據本發明, 提供一種抑制菌體或菌體處理物之凝聚之方法,其係於含有菌體及/或菌體處理物、及大豆多醣類之飲料中,滿足以下之式11~13, 式11:0.22質量%<(菌體及/或菌體處理物之含量)(質量%)≦0.35質量%; 式12:0.044質量%<大豆多醣類含量(質量%)≦1質量%; 式13:0.2≦{大豆多醣類含量(質量%)/(菌體及/或菌體處理物之含量)(質量%)}。 [發明之效果]Furthermore, according to the present invention, Provided is a method for inhibiting the aggregation of bacterial cells or bacterial cell processed substances in a beverage containing bacterial cells and/or bacterial cell processed substances, and soybean polysaccharides, and satisfying the following formulas 11 to 13, Formula 11: 0.22 mass % < (content of bacterial cells and/or bacterial cell treated materials) (mass %) ≦ 0.35 mass %; Formula 12: 0.044 mass % < soybean polysaccharide content (mass %) ≦ 1 mass %; Formula 13: 0.2 ≦ {soybean polysaccharide content (mass %)/(content of bacterial cells and/or bacterial cell treatments) (mass %)}. [Effects of the invention]
根據本發明,可有效抑制飲料之菌體或菌體處理物之凝聚。According to the present invention, the aggregation of bacterial cells or bacterial cell processed materials in beverages can be effectively suppressed.
以下,對本發明之實施形態進行說明。再者,於本說明書中,只要未特別說明,則各成分之含量表示相對於飲料整體之質量的質量(質量%)。Hereinafter, embodiments of the present invention will be described. In addition, in this specification, unless otherwise stated, the content of each component represents the mass (mass %) with respect to the mass of the whole drink.
<飲料> 本發明之飲料包含菌體、菌體處理物之至少一者,亦可均包含。作為飲料之種類,例如可列舉:功能性飲料、非乳性飲料、運動飲料、似水飲料等。又,不含源自乳之蛋白質之飲料亦為本發明之對象。 再者,所謂運動飲料,係大量包含礦物成分,且主要為了快速補充因運動等以而汗之形式失去之水分或礦物而飲用之飲料。又,於似水飲料中,亦包括乍一觀看如水者、或因乳化劑而帶有濁度之飲料。進而,該等中以感受到果實或乳製品之風味、甜味之方式設計之飲料亦成為本發明之對象。<Drinks> The beverage of the present invention contains at least one of bacterial cells and bacterial cell processed materials, or may contain both. Examples of types of beverages include functional beverages, non-dairy beverages, sports drinks, water-like beverages, and the like. In addition, beverages that do not contain milk-derived protein are also targeted by the present invention. In addition, so-called sports drinks are drinks that contain a large amount of mineral components and are mainly consumed to quickly replenish water or minerals lost in the form of sweating due to exercise. Furthermore, water-like beverages also include beverages that look like water at first glance or have turbidity due to emulsifiers. Furthermore, among these beverages designed in such a way that the flavor and sweetness of fruits or dairy products can be felt, the invention is also targeted.
本發明之飲料係將「(菌體及/或菌體處理物之含量)(質量%)」、「大豆多醣類含量(質量%)」及「大豆多醣類含量(質量%)/(菌體及/或菌體處理物之含量)(質量%)」設為特定之範圍。具體而言如下。The beverage of the present invention combines "(content of bacterial cells and/or processed bacterial cells) (mass %)", "soybean polysaccharide content (mass %)" and "soybean polysaccharide content (mass %)/( Content of bacterial cells and/or bacterial cell treated materials) (mass %)" is set to a specific range. The details are as follows.
本發明之飲料滿足條件1~3。 式1:0.001質量%≦(菌體及/或菌體處理物之含量)(質量%)≦0.22質量% 式2:0.0001質量%≦大豆多醣類含量(質量%)≦1質量% 式3:0.1≦{大豆多醣類含量(質量%)/(菌體及/或菌體處理物之含量)(質量%)} 又,式3之較佳上限為1000。The beverage of the present invention satisfies conditions 1 to 3. Formula 1: 0.001 mass % ≦ (content of bacterial cells and/or bacterial cell treated materials) (mass %) ≦ 0.22 mass % Formula 2: 0.0001 mass % ≦ soybean polysaccharide content (mass %) ≦ 1 mass % Formula 3: 0.1≦{Soybean polysaccharide content (mass %)/(content of bacterial cells and/or bacterial cell treatments) (mass %)} In addition, the preferred upper limit of Formula 3 is 1,000.
又,本發明之飲料滿足條件4~6。 式4:0.22質量%<(菌體及/或菌體處理物之含量)(質量%)≦0.35質量% 式5:0.044質量%<大豆多醣類含量(質量%)≦1質量% 式6:0.2≦{大豆多醣類含量(質量%)/(菌體及/或菌體處理物之含量)(質量%)} 又,式6之較佳上限為4.54。Furthermore, the beverage of the present invention satisfies conditions 4 to 6. Formula 4: 0.22 mass % < (content of bacterial cells and/or bacterial cell treated materials) (mass %) ≦ 0.35 mass % Formula 5: 0.044 mass % < soybean polysaccharide content (mass %) ≦ 1 mass % Formula 6: 0.2≦{Soybean polysaccharide content (mass %)/(content of bacterial cells and/or bacterial cell treatments) (mass %)} In addition, the preferred upper limit of Formula 6 is 4.54.
藉由上述{大豆多醣類含量(質量%)/(菌體及/或菌體處理物之含量)(質量%)}設為下限值以上,而一面使飲料之可口性之平衡良好,一面容易提高抑制凝聚之效果。 另一方面,藉由上述{大豆多醣類含量(質量%)/(菌體及/或菌體處理物之含量)(質量%)}設為上限值以下,可一面保持飲料之風味一面提高飲料之功能或可口性,並且可保持良好之抑制凝聚之效果。By setting the above {soybean polysaccharide content (mass %)/(content of bacterial cells and/or bacterial cell processed materials) (mass %)} above the lower limit value, the palatability of the beverage can be well-balanced, On the one hand, it is easy to improve the effect of inhibiting aggregation. On the other hand, by setting the above {soybean polysaccharide content (mass %)/(content of bacterial cells and/or bacterial cell processed materials) (mass %)} below the upper limit, the flavor of the beverage can be maintained while Improve the function or palatability of beverages, and maintain good aggregation-inhibiting effects.
即,本發明之飲料藉由特別指定「(菌體及/或菌體處理物之含量)(質量%)」、「大豆多醣類含量(質量%)」及「大豆多醣類含量(質量%)/(菌體及/或菌體處理物之含量)(質量%)」之範圍,而提高菌體、及菌體處理物之分散性。換言之,本發明發現了使菌體及/或菌體處理物與大豆多醣類之摻合適當時,可獲得針對菌體及/或菌體處理物的優異之抑制凝聚之效果之條件。That is, the beverage of the present invention is made by specifically specifying "(content of bacterial cells and/or processed bacterial cells) (mass %)", "soybean polysaccharide content (mass %)" and "soybean polysaccharide content (mass %)" %)/(content of bacterial cells and/or bacterial cell treated materials) (mass %)" range to improve the dispersion of bacterial cells and bacterial cell treated materials. In other words, the present invention has discovered the conditions under which an excellent aggregation-inhibiting effect on the bacterial cells and/or the treated bacterial cells can be obtained by properly blending the bacterial cells and/or the bacterial cell treated material with soybean polysaccharides.
[菌體、菌體處理物] 作為菌體,例如可列舉:乳酸菌之菌體、酵母之菌體。又,於乳酸菌中,除了乳酸桿菌、乳酸球菌以外,亦包括作為廣義之乳酸菌之雙歧桿菌。作為乳酸菌之菌體,只要為通常用於飲食品者,則並無限定,但例如可列舉屬於乳酸桿菌(Lactobacillus)屬、雙叉桿菌(Bifidobacterium)屬、白念珠球菌(Leuconostoc)屬、乳酸球菌(Lactococcus)屬、小球菌(Pediococcus)屬、腸球菌(Enterococcus)屬、鏈球菌(Streptococcus)屬、魏斯氏菌(Weissella)屬等之乳酸菌之菌體。該等菌體可使用1種,亦可混合使用2種以上。 例如,L.acidophilus乳酸菌體可藉由國際公開公報第2012/029825號中記載之方法獲得,L.gasseri之乳酸菌體可藉由日本特開2015-96542號公報中記載之方法獲得,L.amylovorus之菌體可藉由國際公開第2011/155518號中記載之方法獲得,乳酸菌之破碎菌體等處理物可藉由國際公開第2011/155518號中記載之方法獲得。[Bacterial cells, bacterial cell treated materials] Examples of bacterial cells include bacterial cells of lactic acid bacteria and bacterial cells of yeast. Moreover, lactic acid bacteria include, in addition to lactobacilli and lactococci, bifidobacteria which are lactic acid bacteria in a broad sense. The bacterial cells of the lactic acid bacteria are not limited as long as they are commonly used in food and drink, but examples include Lactobacillus genus, Bifidobacterium genus, Leuconostoc genus, Lactococcus genus (Lactococcus), Pediococcus (Pediococcus), Enterococcus (Enterococcus), Streptococcus (Streptococcus), Weissella (Weissella) and other lactic acid bacteria. One type of these bacterial cells may be used, or two or more types may be mixed and used. For example, lactic acid bacteria of L. acidophilus can be obtained by the method described in International Publication No. 2012/029825, lactic acid bacteria of L. gasseri can be obtained by the method described in Japanese Patent Application Publication No. 2015-96542, L. amylovorus The bacterial cells can be obtained by the method described in International Publication No. 2011/155518, and the processed products such as the broken cells of lactic acid bacteria can be obtained by the method described in International Publication No. 2011/155518.
所謂菌體處理物,可列舉將如上所述之菌體破碎而得之菌體破碎物。又,用於本發明之菌體、菌體處理物可例示藉由冷凍乾燥或噴霧乾燥而獲得者。Examples of the bacterial cell processed product include crushed bacterial cells obtained by crushing the above-mentioned bacterial cells. In addition, examples of the bacterial cells and bacterial cell treated products used in the present invention are those obtained by freeze-drying or spray-drying.
(菌體及/或菌體處理物)之合計之含量(質量%)之下限值較佳為相對於飲料整體而依序為0.001質量%以上、0.002質量%以上、0.003質量%以上、0.005質量%以上、0.01質量%以上、0.02質量%以上、0.05質量%以上、0.06質量%以上、0.07質量%以上、0.08質量%以上、0.09質量%以上、0.1質量%以上、0.15質量%以上。藉此,有效獲得菌體之功能。The lower limit of the total content (mass %) of (microbial cells and/or microbial cell treated substances) is preferably 0.001 mass % or more, 0.002 mass % or more, 0.003 mass % or more, and 0.005 with respect to the entire beverage. Mass % or more, 0.01 mass % or more, 0.02 mass % or more, 0.05 mass % or more, 0.06 mass % or more, 0.07 mass % or more, 0.08 mass % or more, 0.09 mass % or more, 0.1 mass % or more, 0.15 mass % or more. In this way, the functions of the bacteria are effectively obtained.
另一方面,(菌體及/或菌體處理物)之合計之含量(質量%)之上限值較佳為依序為0.35質量%以下、0.3質量%以下、0.25質量%以下、0.22質量%以下。藉此,可使良好之抑制凝聚之效果與飲料之可口性之平衡變得良好。On the other hand, the upper limit of the total content (mass %) of (bacterial cells and/or bacterial cell treated materials) is preferably 0.35 mass % or less, 0.3 mass % or less, 0.25 mass % or less, and 0.22 mass % or less. %the following. Thereby, a good balance can be achieved between the good aggregation-inhibiting effect and the palatability of the drink.
[大豆多醣類] 大豆多醣類係自大豆提取之水溶性之多醣類,主成分為半纖維素。作為大豆多醣類之市售品,例如可列舉SOYAFIVE SZ-R100(不二製油公司製造)、SM-1200(三榮源FFI股份有限公司製造)等。[Soybean polysaccharides] Soybean polysaccharides are water-soluble polysaccharides extracted from soybeans, and their main component is hemicellulose. Examples of commercially available soybean polysaccharides include SOYAFIVE SZ-R100 (manufactured by Fuji Oil Co., Ltd.), SM-1200 (manufactured by Saneigen FFI Co., Ltd.), and the like.
大豆多醣類之含量(質量%)係根據上述(菌體及/或菌體處理物)之合計之含量(質量%)進行設定。 具體而言,於(菌體及/或菌體處理物)之合計之含量(質量%)滿足式1之情形時,就獲得良好之抑制凝聚之效果之觀點而言,大豆多醣類之含量(質量%)之較佳之下限值依序為0.0001質量%以上、0.0025質量%以上、0.003質量%以上、0.004質量%以上、0.005質量%以上、0.007質量%以上、0.01質量%以上。另一方面,大豆多醣類之含量(質量%)之上限值為1質量%以下,但就保持飲料所具有之功能或風味、可口性之觀點而言,較佳為依序為0.9質量%以下、0.8質量%以下、0.7質量%以下。 又,於(菌體及/或菌體處理物)之合計之含量(質量%)滿足式4之情形時,就獲得良好之抑制凝聚之效果之觀點而言,大豆多醣類之含量(質量%)之較佳之下限值為大於0.044質量%,更佳為0.05質量%以上。另一方面,大豆多醣類之含量(質量%)之上限值為1質量%以下,但就保持飲料所具有之功能或風味、可口性之觀點而言,較佳為依序為0.9質量%以下、0.8質量%以下、0.7質量%以下。The content (mass %) of soybean polysaccharides is set based on the total content (mass %) of the above (bacterial cells and/or bacterial cell treated materials). Specifically, when the total content (mass %) of (microbial cells and/or microbial cell treated materials) satisfies Formula 1, the content of soybean polysaccharides is obtained from the viewpoint of obtaining a good aggregation-inhibiting effect. Preferable lower limits (mass %) are, in order, 0.0001 mass % or more, 0.0025 mass % or more, 0.003 mass % or more, 0.004 mass % or more, 0.005 mass % or more, 0.007 mass % or more, and 0.01 mass % or more. On the other hand, the upper limit of the content (mass %) of soybean polysaccharides is 1 mass % or less, but from the viewpoint of maintaining the functions, flavors, and palatability of the beverage, it is preferably 0.9 mass % or less. % or less, 0.8 mass% or less, 0.7 mass% or less. Furthermore, when the total content (mass %) of (microbial cells and/or microbial cell treated materials) satisfies Formula 4, from the viewpoint of obtaining a good aggregation-inhibiting effect, the content (mass %) of soybean polysaccharides %) is more than 0.044 mass %, more preferably 0.05 mass % or more. On the other hand, the upper limit of the content (mass %) of soybean polysaccharides is 1 mass % or less, but from the viewpoint of maintaining the functions, flavors, and palatability of the beverage, it is preferably 0.9 mass % or less. % or less, 0.8 mass% or less, 0.7 mass% or less.
又,本發明之飲料較佳為波長660 nm時之吸光度滿足以下之式7。藉由吸光度為下限值以上且上限值以下,而成為外觀良好之飲料。 式7:0≦吸光度≦3.3 上述吸光度除了能以一般之飲料之外觀之形式採用以外,還可採用3.3以下作為功能性飲料、運動飲料、似水飲料等之波長660 nm時之吸光度。因此,本發明之飲料可製成一般之飲料、功能性飲料、運動飲料、似水飲料等飲料進行流通、銷售。 吸光度可使用分光光度計測定各飲料之波長660 nm時之吸光度。Furthermore, it is preferable that the absorbance of the beverage of the present invention at a wavelength of 660 nm satisfies the following formula 7. When the absorbance is above the lower limit value and below the upper limit value, it becomes a drink with a good appearance. Formula 7: 0≦Absorbance≦3.3 In addition to the above-mentioned absorbance being used in the form of the appearance of general beverages, the absorbance at a wavelength of 660 nm of functional drinks, sports drinks, water-like drinks, etc. can also be used as 3.3 or less. Therefore, the beverage of the present invention can be distributed and sold in the form of general beverages, functional beverages, sports drinks, water-like beverages and other beverages. The absorbance of each beverage can be measured using a spectrophotometer at a wavelength of 660 nm.
[飲料之物性、性狀] 本發明之飲料亦可為非碳酸飲料,但亦可使其含有二氧化碳而製成碳酸飲料。於該情形時,所添加之二氧化碳之氣體容量並無特別限定,較佳為1.0以上且5.0以下,更佳為2.0以上且4.0以下。再者,氣體容量係指於一個大氣壓、20℃碳酸飲料中溶解之二氧化碳之體積除以碳酸飲料之體積所得的值。[Physical properties and properties of beverages] The beverage of the present invention may be a non-carbonated beverage, or may be made into a carbonated beverage by containing carbon dioxide. In this case, the gas capacity of carbon dioxide to be added is not particularly limited, but is preferably 1.0 or more and 5.0 or less, more preferably 2.0 or more and 4.0 or less. Furthermore, the gas capacity refers to the value obtained by dividing the volume of carbon dioxide dissolved in a carbonated drink at one atmospheric pressure and 20°C by the volume of the carbonated drink.
本發明之飲料之pH(20℃)只要為弱酸性,則並無特別限定,較佳為6.5以下,更佳為6以下,進而較佳為5.5以下。另一方面,飲料之pH(20℃)較佳為3以上,更佳為3.2以上。The pH (20° C.) of the beverage of the present invention is not particularly limited as long as it is weakly acidic, but is preferably 6.5 or less, more preferably 6 or less, and even more preferably 5.5 or less. On the other hand, the pH (20°C) of the beverage is preferably 3 or more, more preferably 3.2 or more.
於製造本發明之飲料之情形時,只要藉由所使用之原材料而pH成為上述範圍,則無須調整pH,於並非上述範圍之情形時,使用pH調整劑進行pH調整。下限值較佳為3.0以上。When producing the beverage of the present invention, as long as the pH is within the above range due to the raw materials used, there is no need to adjust the pH. If the pH is outside the above range, pH adjustment is performed using a pH adjuster. The lower limit value is preferably 3.0 or more.
作為pH調整劑,使用一般用作酸味劑之有機或無機之食用酸或該等之鹽即可,例如可列舉:檸檬酸、蘋果酸、酒石酸、乙酸、植酸、乳酸、反丁烯二酸、琥珀酸、及葡萄糖酸等有機酸,磷酸等無機酸,或該等之鈉鹽、鈣鹽或鉀鹽等。pH調整劑之使用量只要為可設為所期望之pH,且不影響飲料之風味之範圍,則並無特別限定。As a pH adjuster, organic or inorganic edible acids or their salts commonly used as sour agents may be used. Examples include: citric acid, malic acid, tartaric acid, acetic acid, phytic acid, lactic acid, and fumaric acid. , succinic acid, gluconic acid and other organic acids, phosphoric acid and other inorganic acids, or their sodium salts, calcium salts or potassium salts, etc. The usage amount of the pH adjuster is not particularly limited as long as the pH can be set to a desired pH without affecting the flavor of the beverage.
本發明之飲料之Brix值並無特別限定,就一面使風味良好一面成為適度之卡路里之觀點而言,較佳為0.1~35,更佳為0.5~20。The Brix value of the beverage of the present invention is not particularly limited, but from the viewpoint of achieving a good flavor while having moderate calories, it is preferably 0.1 to 35, more preferably 0.5 to 20.
Brix值(Bx)係20℃時之糖用折射計之讀數,例如係指使用數位折射計Rx-5000α(Atago公司製造)於20℃測得之值(Bx)。Brix value (Bx) is the reading of a sugar refractometer at 20°C. For example, it refers to the value (Bx) measured at 20°C using a digital refractometer Rx-5000α (manufactured by Atago Company).
本發明之飲料可於不損及本發明之效果之範圍內摻合通常用於一般之飲料的其他成分。The beverage of the present invention may be blended with other ingredients commonly used in ordinary beverages within a range that does not impair the effects of the present invention.
[其他成分] 例如,本發明之飲料亦可使用上述大豆多醣類以外之增稠多醣類。所謂增稠多醣類,係對水系液體賦予黏性之多醣類,係增稠穩定劑之一種。作為增稠多醣類,為上述大豆多醣類以外,例如可列舉:瓊脂、明膠、果膠、鹿角菜膠、半乳甘露聚糖、聚葡甘露糖(glucomannan)、三仙膠、瓜爾膠、大瑪琳籽膠(tamarind seed gum)、羅望子膠(tamarind gum)、阿拉伯樹膠、塔拉膠及海藻酸鹽等。其中較佳為選自果膠、鹿角菜膠、刺槐豆膠、三仙膠、瓜爾膠、大瑪琳籽膠、羅望子膠、及阿拉伯樹膠中之1種或2種以上。[Other ingredients] For example, the beverage of the present invention may use thickening polysaccharides other than the above-mentioned soybean polysaccharides. The so-called thickening polysaccharides are polysaccharides that impart viscosity to aqueous liquids and are a type of thickening stabilizer. Examples of the thickening polysaccharides other than the above-mentioned soybean polysaccharides include agar, gelatin, pectin, carrageenan, galactomannan, polyglucomannan, gum, and guar. Gum, tamarind seed gum, tamarind gum, acacia gum, tara gum and alginate, etc. Among them, one or more types selected from the group consisting of pectin, carrageenan, locust bean gum, sanxian gum, guar gum, marin seed gum, tamarind gum, and gum arabic are preferred.
例如,為了對本發明之飲料賦予甜味,亦可摻合單糖(葡萄糖、果糖、木糖、半乳糖等)、二糖(蔗糖、麥芽糖、乳糖、海藻糖、異麥芽酮糖等)、寡醣(低聚果糖、麥芽寡糖、異麥芽寡糖、半乳寡糖、偶合糖等)、糖醇(赤藻糖醇、木糖醇、山梨糖醇、麥芽糖醇、乳糖醇、還原異麥芽酮糖、還原飴糖等)、果糖葡萄糖液糖等異構化糖等。又,亦可摻合蔗糖素、阿斯巴甜、乙醯磺胺酸鉀、甜菊等高甜味度甜味劑。For example, in order to impart sweetness to the beverage of the present invention, monosaccharides (glucose, fructose, xylose, galactose, etc.), disaccharides (sucrose, maltose, lactose, trehalose, isomaltulose, etc.) may be blended. Oligosaccharides (fructooligosaccharides, malto-oligosaccharides, isomaltooligosaccharides, galactooligosaccharides, coupled sugars, etc.), sugar alcohols (erythritol, xylitol, sorbitol, maltitol, lactitol, Reduced isomaltulose, reduced maltose, etc.), isomerized sugars such as fructose glucose liquid sugar, etc. In addition, high-intensity sweeteners such as sucralose, aspartame, acesulfame potassium, and stevia may be blended.
又,例如於本發明之飲料中可摻合果汁等。Furthermore, for example, fruit juice, etc. may be blended with the beverage of the present invention.
於本發明之飲料中,亦可添加飲料所容許之各種添加劑,例如抗氧化劑(維生素E、抗壞血酸、半胱胺酸鹽酸等)、香料(檸檬香料、橘子香料、葡萄香料、桃香料、蘋果香料等)、色素(類胡蘿蔔素色素、花青苷色素、紅花色素、梔子色素、焦糖色素、各種合成著色劑等)等。又,亦可期待健康功能之增強,而添加維生素類(維生素B群、維生素C、維生素E、維生素D等)、礦物類(鈣、鉀、鎂等)、食物纖維等各種功能成分。In the beverage of the present invention, various additives allowed by the beverage can also be added, such as antioxidants (vitamin E, ascorbic acid, cysteine hydrochloric acid, etc.), spices (lemon spice, orange spice, grape spice, peach spice, apple spice) Flavors, etc.), pigments (carotenoid pigments, anthocyanin pigments, safflower pigments, gardenia pigments, caramel pigments, various synthetic colorants, etc.), etc. In addition, in order to enhance health functions, various functional ingredients such as vitamins (vitamin B complex, vitamin C, vitamin E, vitamin D, etc.), minerals (calcium, potassium, magnesium, etc.), dietary fiber, etc. can be added.
<飲料之製造方法> 本發明之飲料可藉由公知之方法進行製造。具體而言,可預先對上述菌體或菌體處理物進行均勻化處理,其後與水、大豆多醣類等其他成分一同混合而製備飲料,並進行加熱殺菌,從而製備飲料。<How to make drinks> The beverage of the present invention can be produced by known methods. Specifically, the above-mentioned bacterial cells or treated bacterial cells are homogenized in advance, and then mixed with other ingredients such as water and soybean polysaccharides to prepare a beverage, and then heat-sterilized to prepare a beverage.
<容器裝飲料> 本發明之容器裝飲料係將上述飲料填充至容器而成。<Containerized drinks> The container-packed beverage of the present invention is obtained by filling the above-mentioned beverage into a container.
作為容器,可適當選擇使用可將飲料密封之公知之容器。例如可列舉:由玻璃、紙、塑膠(聚對苯二甲酸乙二酯(PET)等)、鋁、及鋼等單獨成分或該等之複合材料或積層材料所構成之密封容器。又,容器之種類並無特別限定,例如可列舉:PET瓶、鋁罐、鋼罐、紙袋、冰鎮杯、瓶等。進而,就自外觀觀察飲料而可確認透明性、顏色等觀點而言,作為容器之顏色,較佳為透明,更佳為無色透明。又,就使用性、流通性、便攜性等觀點而言,較佳為PET瓶。As a container, a well-known container capable of sealing a beverage can be appropriately selected and used. Examples include sealed containers made of individual components such as glass, paper, plastic (polyethylene terephthalate (PET), etc.), aluminum, and steel, or composite materials or laminated materials thereof. In addition, the type of container is not particularly limited, and examples include: PET bottles, aluminum cans, steel cans, paper bags, ice cups, bottles, etc. Furthermore, from the viewpoint that the transparency, color, etc. can be confirmed by observing the appearance of the beverage, the color of the container is preferably transparent, and more preferably colorless and transparent. In addition, from the viewpoints of usability, circulation, portability, etc., PET bottles are preferred.
作為容器之容量,基於飲料之功能、可口性、菌體或菌體處理物之摻合量等適當進行設定,例如可列舉50 ml~2 L。The capacity of the container is appropriately set based on the function and palatability of the beverage, the blending amount of the bacterial cells or the bacterial cell treated material, etc., and may be, for example, 50 ml to 2 L.
<抑制菌體或菌體處理物之凝聚之方法> 本發明之抑制菌體或菌體處理物之凝聚之方法係於含有菌體及/或菌體處理物、及大豆多醣類之飲料中,滿足以下之式8~10。 式8:0.001質量%≦(菌體及/或菌體處理物之含量)(質量%)≦0.22質量% 式9:0.0001質量%≦大豆多醣類含量(質量%)≦1質量% 式10:0.1≦{大豆多醣類含量(質量%)/(菌體及/或菌體處理物之含量)(質量%)} 再者,作為較佳之實施形態,較佳為不含源自乳之蛋白質之飲料。又,式10之較佳上限為1000。 藉此,獲得良好之抑制菌體或菌體處理物之凝聚之效果。<Methods to inhibit the aggregation of bacterial cells or bacterial cell treated materials> The method of inhibiting the aggregation of bacterial cells or bacterial cell processed materials of the present invention is to satisfy the following formulas 8 to 10 in a beverage containing bacterial cells and/or bacterial cell processed materials, and soybean polysaccharides. Formula 8: 0.001 mass % ≦ (content of bacterial cells and/or bacterial cell treated materials) (mass %) ≦ 0.22 mass % Formula 9: 0.0001 mass % ≦ soybean polysaccharide content (mass %) ≦ 1 mass % Formula 10: 0.1≦{Soybean polysaccharide content (mass %)/(content of bacterial cells and/or bacterial cell treatments) (mass %)} Furthermore, as a preferred embodiment, a beverage containing no milk-derived protein is preferred. In addition, the preferable upper limit of Formula 10 is 1000. Thereby, a good effect of inhibiting the aggregation of bacterial cells or bacterial cell treated materials is obtained.
又,本發明之抑制菌體或菌體處理物之凝聚之方法係於含有菌體及/或菌體處理物、及大豆多醣類之飲料中,滿足以下之式11~13。 式11:0.22質量%<(菌體及/或菌體處理物之含量)(質量%)≦0.35質量% 式12:0.044質量%<大豆多醣類含量(質量%)≦1質量% 式13:0.2≦{大豆多醣類含量(質量%)/(菌體及/或菌體處理物之含量)(質量%)} 再者,作為較佳之實施形態,較佳為不含源自乳之蛋白質之飲料。又,式13之較佳上限為4.54。Furthermore, the method of inhibiting the aggregation of bacterial cells or bacterial cell processed materials of the present invention is to satisfy the following formulas 11 to 13 in a beverage containing bacterial cells and/or bacterial cell processed materials, and soybean polysaccharides. Formula 11: 0.22 mass % < (content of bacterial cells and/or bacterial cell treated materials) (mass %) ≦ 0.35 mass % Formula 12: 0.044 mass % < soybean polysaccharide content (mass %) ≦ 1 mass % Formula 13: 0.2≦{Soybean polysaccharide content (mass %)/(content of bacterial cells and/or bacterial cell treatments) (mass %)} Furthermore, as a preferred embodiment, a beverage containing no milk-derived protein is preferred. In addition, the preferable upper limit of Equation 13 is 4.54.
以上,對本發明之實施形態進行了敍述,但該等實施形態為本發明之例示,亦可採用上述以外之各種構成。 實施例The embodiments of the present invention have been described above. However, these embodiments are examples of the present invention, and various configurations other than those described above may be adopted. Example
其次,藉由實施例對本發明詳細地進行說明,但本發明之內容並不限於實施例。Next, the present invention will be described in detail through examples, but the content of the present invention is not limited to the examples.
[飲料之原料] ・乳酸菌粉末A:菌種L.acidophilus ・乳酸菌粉末B(菌體處理物):菌種L.amylovorus ・乳酸菌粉末C:菌種L.gasseri 再者,乳酸菌粉末B(菌體處理物):菌種L.amylovorus係使用國際公開第2011/155518號中記載之菌體破碎菌粉末。 又,均使用藉由冷凍乾燥而獲得之乳酸菌粉末(A、B、C)。 ・大豆多醣類:「製品名SM1200」三榮源FFI股份有限公司製造[Ingredients for drinks] ・Lactic acid bacteria powder A: strain L.acidophilus ・Lactic acid bacteria powder B (cell treated product): strain L. amylovorus ・Lactic acid bacteria powder C: strain L.gasseri In addition, lactic acid bacteria powder B (cell treated product): The bacterial cell disrupted bacteria powder described in International Publication No. 2011/155518 was used as the strain L. amylovorus. In addition, lactic acid bacteria powder (A, B, C) obtained by freeze-drying was used. ・Soybean polysaccharides: "Product name SM1200" manufactured by Samyoungyuan FFI Co., Ltd.
[實驗1] <No.1> 將乳酸菌粉末A投入水中,以成為1%濃度之方式進行製備,以5000 rpm進行5分鐘均質處理,製作含有乳酸菌之液體。 將5質量%之晶粒砂糖、0.15質量%之檸檬酸酐、0.07質量%之檸檬酸三鈉、及0.0001質量%之大豆多醣類、以及上述所獲得之加入有乳酸菌之溶液混合,以成為乳酸菌粉末0.001質量%之方式製備飲料。繼而,進行加熱殺菌,並填充至PET瓶(內容量100 ml),從而獲得容器裝飲料。[Experiment 1] <No.1> The lactic acid bacteria powder A was put into water to prepare a concentration of 1%, and homogenized at 5000 rpm for 5 minutes to prepare a liquid containing lactic acid bacteria. Mix 5% by mass of crystal sugar, 0.15% by mass of citric anhydride, 0.07% by mass of trisodium citrate, 0.0001% by mass of soybean polysaccharides, and the above-obtained solution containing lactic acid bacteria to form lactic acid bacteria Beverages were prepared using 0.001 mass% powder. Then, it is heated and sterilized, and a PET bottle (inner capacity: 100 ml) is filled to obtain a containerized beverage.
<No.2~No.37> 以成為表1所示之組成之方式選擇乳酸菌粉末,調整乳酸菌粉末及大豆多醣類之摻合量,除此以外,以與No.1相同之方式獲得容器裝飲料。<No.2~No.37> A containerized beverage was obtained in the same manner as No. 1, except that the lactic acid bacteria powder was selected so as to have the composition shown in Table 1 and the blending amounts of the lactic acid bacteria powder and soybean polysaccharides were adjusted.
<No.38~No.47> 以成為表2、表3所示之組成之方式選擇乳酸菌粉末,調整乳酸菌粉末及大豆多醣類之摻合量,除此以外,以與No.1相同之方式獲得容器裝飲料。<No.38~No.47> A containerized beverage was obtained in the same manner as No. 1 except that the lactic acid bacteria powder was selected so as to have the composition shown in Table 2 and Table 3 and the blending amounts of the lactic acid bacteria powder and soybean polysaccharides were adjusted.
針對上述所獲得之容器裝飲料進行以下之測定及評價。將結果示於表1~3。 [測定] ・糖度(白利糖度(Brix)值):使用Atago股份有限公司製造之糖用折射計「RX-5000α」測定糖度。飲料之液溫設為20℃。 ・pH:設為利用東亞DKK公司製造之GST-5741C所測得之值(20℃)。The following measurement and evaluation were performed on the packaged beverage obtained above. The results are shown in Tables 1 to 3. [Measurement] ・Brix content (Brix value): Measure the sugar content using the sugar refractometer "RX-5000α" manufactured by Atago Co., Ltd. The liquid temperature of the beverage is set to 20°C. ・pH: Set to the value measured using GST-5741C manufactured by East Asia DKK Co., Ltd. (20°C).
[評價] 針對所獲得之容器裝飲料,於20℃靜止48小時後,目視觀察外觀,進行以下之評價。 [沈澱之有無] 1 有沈澱 2 無沈澱 [沈澱之凝聚狀態] 1 微粒子狀沈澱〔無絮狀凝聚物(絮狀沈澱物)〕(☆) 2 基本上無絮狀凝聚物(沈澱物)但不可謂完全不存在(◎) 3 有少量絮狀凝聚物(沈澱物)(○) 4 有絮狀凝聚物(沈澱物)(△)[evaluation] After the obtained packaged beverage was allowed to stand at 20° C. for 48 hours, the appearance was visually observed, and the following evaluation was performed. [The presence or absence of precipitation] 1 There is precipitation 2 No precipitation [Condensed state of precipitation] 1 Fine particulate precipitation [no flocculent aggregates (flocculated sediment)] (☆) 2 There is basically no floc aggregate (precipitate) but it cannot be said to be completely absent (◎) 3 There is a small amount of floc aggregate (sediment) (○) 4 There are flocculent aggregates (precipitates) (△)
[表1]
[表2]
[表3]
根據以上之結果,於[沈澱之凝聚狀態]中,1微粒子狀沈澱〔無絮狀凝聚物(絮狀沈澱物)〕(☆)、及2基本上無絮狀凝聚物(沈澱物)但不可謂完全不存在(◎)之評價之飲料係判斷為外觀上優異,視為合格。According to the above results, in the [aggregation state of precipitation], 1 is fine particulate precipitation [no flocculent aggregates (flocculated precipitates)] (☆), and 2 is basically free of flocculent aggregates (precipitates) but not Beverages with absolutely no (◎) evaluation were judged to be excellent in appearance and deemed to have passed the test.
[實驗2]一般之飲料之吸光度之驗證 於混合有5質量%之砂糖、0.15質量%之檸檬酸酐、0.07質量%之檸檬酸三鈉之調合液中,以成為表4所示之摻合率之方式摻合市售品「NEOCLA N-6」(三榮源FFI股份有限公司製造),製作可用於一般之飲料、功能性飲料、運動飲料、及似水飲料等飲料之樣本飲料,並測定吸光度。 ・吸光度之測定條件 測定波長:660 nm 測定設備:U-3900H(HITACHI製造) 測定條件:石英池、光路長10 mm[Experiment 2] Verification of absorbance of general beverages The commercial product "NEOCLA N- 6" (manufactured by Sanrongyuan FFI Co., Ltd.), prepare sample beverages that can be used for general beverages, functional beverages, sports drinks, water-like beverages and other beverages, and measure the absorbance. ・Absorbance measurement conditions Measurement wavelength: 660 nm Measuring equipment: U-3900H (manufactured by HITACHI) Measurement conditions: quartz cell, optical path length 10 mm
[表4]
樣本飲料於飲料之外觀上均不存在問題。There are no problems with the appearance of the sample drinks.
本申請主張以於2018年4月27日提出申請之日本特願2018-086256號為基礎之優先權,將其揭示之全部內容引入於此。This application claims priority based on Japanese Patent Application No. 2018-086256 filed on April 27, 2018, and the entire disclosure thereof is incorporated herein by reference.
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