TWI811118B - Preparation method and usage of herbal medicine extracts - Google Patents

Abstract

The present invention provides a preparation method and usage of herbal medicine extracts. The herbal medicine extracts include an effective component, which contains an agarwood, gleditsia, gleditsia thorn, cinnamon leaf, camphor tree leaf or a combination thereof. The medicine containing such herbal medicine extracts can be applied for treating coronavirus related symptoms or diseases.

Description

Chinese medicinal material extract and its preparation method and use

The invention relates to the technical field of extracts of traditional Chinese medicinal materials, in particular to extracts of traditional Chinese medicinal materials and its preparation method and application.

Coronavirus (Coronavirus) is a zoonotic RNA virus that spreads between animals and humans. It is spherical or oval in shape under an electron microscope. It is called a coronavirus because of its protruding cystic collagen fibers, which resemble a crown. The genome size of coronaviruses is 26,000 to 32,000 base pairs, making it the largest type of RNA virus with the largest genome.

The novel coronavirus SARS-CoV-2 (originally reported as COVID-19 and officially named SARS-CoV-2 by the Coronavirus Research Group based on kinship, taxonomy, and convention) is a new virus strain, SARS-CoV- 2. The viral spike protein (Spike Protein) binds to human cell surface receptors, and through the 3CL ^protease that is cut from the protein chain produced by viral gene transcription, and then produces various important proteins inside the virus, allowing the virus to continue to infect with copy.

As the novel coronavirus epidemic is raging, common signs of novel coronavirus infection include respiratory symptoms, fever, cough, shortness of breath or difficulty breathing, and even death in more severe cases. Drugs for related symptoms or diseases.

In order to solve the above problems, the present invention provides an extract of Chinese medicinal materials and its preparation method and application. By taking a medicine containing the extract of Chinese medicinal materials, the effect of treating symptoms or diseases related to coronavirus can be achieved.

The invention provides an extract of Chinese medicinal materials, which contains an active ingredient, which is composed of any one of agarwood, saponins, horned thorns, cinnamon leaves, camphor leaves or a combination thereof.

Another embodiment of the present invention provides a preparation method of Chinese herbal extracts, which comprises the following steps: 1. Extraction step: extract any one of agarwood, saponin, horn thorn, cinnamon leaf, camphor leaf or a combination thereof A raw material and ultrapure water are mixed at a weight ratio of 1:5 to 1:10, and heated for extraction to obtain an extract, wherein the extraction temperature is between 90°C and 110°C, and the extraction time is between 1.5 hours and 2.5 hours hours; a filtering step: filtering the extract; and a drying step: vacuum freeze-drying the extract to obtain the Chinese herbal medicine extract.

Another embodiment of the present invention provides a use of the extract of the above-mentioned Chinese medicinal material for preparing a medicament for treating symptoms or diseases related to coronavirus. The active ingredient of the extract of the Chinese medicinal material is cinnamon leaf.

Another embodiment of the present invention provides a use of the above-mentioned Chinese medicinal material extract for preparing a medicament for interfering with SARS-CoV-2 spinin. The active ingredient of the Chinese medicinal material extract is cinnamon leaf.

Another embodiment of the present invention provides a use of the above-mentioned Chinese herbal medicine extract for preparing a medicament for inhibiting the activity of SARS-CoV-2 3CL ^protease . The active ingredient of the Chinese herbal medicine extract is cinnamon leaf.

Another embodiment of the present invention provides an application of using the extract of the above-mentioned Chinese herbal medicine to prepare a medicament for treating symptoms or diseases related to coronavirus.

Another embodiment of the present invention provides a use of the above-mentioned extracts of Chinese herbal medicines for preparing a medicament for interfering with the spike protein of SARS-CoV-2.

Another embodiment of the present invention provides a use of the above-mentioned Chinese herbal medicine extract for preparing a medicament for inhibiting the activity of SARS-CoV-2 3CL ^pro protease.

In this way, by taking medicines containing extracts of Chinese medicinal materials, it can effectively interfere with the binding of Spike Protein of coronavirus to Human Angiotensin-converting Enzyme 2 (hACE2) on the surface of human cells, and Inhibit the activity of 3CL ^pro protease of coronavirus to achieve the effect of treating symptoms or diseases related to coronavirus.

S1: Extraction step

S2: Filtration step

S3: drying step

Fig. 1 is a flow chart of the preparation of the extract of the Chinese herbal medicine extract of the present invention.

Fig. 2 is the result of the intervention analysis of the Chinese herbal extracts in the first embodiment of the present invention on the combination of SARS-CoV-2 spike protein and hACE2 synthesized in vitro.

Fig. 3 is the analysis result of the half-inhibitory concentration IC ₅₀ of the Chinese medicinal material extract in the first embodiment of the present invention on the binding of the SARS-CoV-2 spike protein to hACE2 synthesized in vitro.

Fig. 4 is the analysis result of the half-effect concentration EC ₅₀ of the Chinese medicinal material extract in the first embodiment of the present invention for the combination of SARS-CoV-2 restricted virus and SmBiT-ACE2 expressing cells.

Fig. 5 is the analysis result of the inhibition of the activity of SARS-CoV-2 3CL ^pro protease by the medicinal material extract in the first embodiment of the present invention.

Fig. 6 is the analysis result of half-inhibitory concentration IC ₅₀ of the Chinese herbal extracts in the first embodiment of the present invention on the inhibition of SARS-CoV-2 3CL ^pro protease activity.

Fig. 7 is the analysis result of half-inhibitory concentration IC ₅₀ of the Chinese herbal extracts in the second embodiment of the present invention on the inhibition of SARS-CoV-2 3CL ^pro protease activity.

In order to illustrate the central idea of the present invention expressed in the column of the above-mentioned summary of the invention, it is expressed in specific embodiments.

The invention provides an extract of Chinese medicinal materials and a preparation method thereof. The extract of Chinese medicinal materials contains an active ingredient. Wherein, the active ingredient is composed of any one of agarwood, saponins, horned thorns, cinnamon leaves, camphor leaves or a combination thereof.

In one embodiment of the present invention, the active ingredient is any one single Chinese herbal medicine among agarwood, saponins, horn thorns, cinnamon leaves and camphor leaves.

In one embodiment of the present invention, the active ingredient is any two compound Chinese herbal medicines of agarwood, saponins, horn thorns, cinnamon leaves or camphor leaves, selected from agarwood and saponins, agarwood and horn thorns, agarwood and cinnamon leaves, agarwood Combination with any of camphor leaves, saponins and thorns, saponins and cinnamon leaves, saponins and camphor leaves, thorns and cinnamon leaves, thorns and camphor leaves, and cinnamon leaves and camphor leaves.

In one embodiment of the present invention, the active ingredient is any three compound Chinese herbal medicines among agarwood, saponins, horned thorns, cinnamon leaves or camphor leaves, such as the combination of agarwood, saponins and horned thorns, the combination of agarwood, saponins and cinnamon leaves , or any combination of agarwood, saponins, and camphor leaves.

In one embodiment of the present invention, the active ingredients are agarwood, saponins, horn thorns, cinnamon leaves Or any four kinds of compound Chinese herbal medicines in camphor leaves, such as the combination of agarwood, saponins, horn thorns, and cinnamon leaves, or any combination of saponins, horn thorns, cinnamon leaves, and camphor leaves.

In one embodiment of the present invention, the active ingredients are composed of agarwood, saponins, horn thorns, cinnamon leaves and camphor leaves, and the weight ratio of agarwood, saponins, horn thorns, cinnamon leaves and camphor leaves is 1~5:1~5 : 1~5: 1~5: 1~5 is better, in this embodiment, the weight ratio of agarwood, saponin, horn thorn, cinnamon leaf and camphor leaf is 1:1:1:1:1.

Materials and Material Handling Methods:

1. Materials

(a) Agarwood

Agarwood is taken from the dicotyledonous plant medicine Daphneaceae plant Agarwood or the resinous wood of Agarwood. It has been confirmed that agarwood has obvious antibacterial effect in pharmacology; in this embodiment, the agarwood wood is selected from the white wood agarwood (Aquilaria sinensis) produced in Vietnam or Taiwan, and in other possible embodiments, Vietnam agarwood (Aquilaria sinensis) can also be selected. crassna), or Indonesian agarwood (Aquilaria malaccensis), Yunnan agarwood (Aquilaria yunnanensis) any one of them.

(b) saponins

Gleditsia sinensis is taken from the leguminous plant Gleditsia sinensis, which is the mature fruit pod of Gleditsia sinensis. Gleditsia sinensis has the functions of dispelling stubborn phlegm, opening and closing the orifices, dispelling wind and killing insects. Has an inhibitory effect on pathogens.

(c) horn spines

The horn thorns are taken from the leguminous plant acacia, which is the thorns of the acacia. The horn thorns have the functions of reducing swelling, removing poison, draining pus, and killing insects. The horn thorns can extract flavonoids (Flavonoids), phenols (Phenols), and amino groups. Acid (Amino Acid), the horn thorn extract has antibacterial and disinfection pharmacologically.

(d) Cinnamon leaves

Cinnamon leaves are taken from Cinnamomum osmophloeum, a plant in the Lauraceae family. It has the functions of dispelling cold, relieving pain, promoting blood circulation, and invigorating the stomach. The extract of cinnamon leaves can inhibit the growth of bacteria pharmacologically, thereby achieving the effect of antibacterial and disinfection.

(e) camphor leaves

Camphor leaves are taken from Cinnamomum camphora, a plant of the Lauraceae family. It has the functions of expelling wind, dehumidification, detoxification, and killing insects. The extract of camphor leaves has antibacterial and disinfecting effects in pharmacology.

2. Material handling method:

(a) Wash the agarwood, saponins, horn thorns, cinnamon leaves and camphor leaves separately, and place them to dry in the shade overnight.

(b) Then dry the agarwood, saponins, horn thorns, cinnamon leaves, and camphor leaves in an oven, wherein the water content of agarwood, saponins, horn thorns, cinnamon leaves, and camphor leaves is between 5% and 20% after drying Preferably, the water content of agarwood, saponins, horn thorns, cinnamon leaves and camphor leaves after drying is 10%.

(c) Grind agarwood, saponins, horn thorns, cinnamon leaves, and camphor leaves with a crusher, wherein the thickness of agarwood, saponins, horn thorns, cinnamon leaves, and camphor leaves ranges from 10 mesh to 30 mesh Mesh is the best, and the fineness of agarwood, saponins, horn thorns, cinnamon leaves and camphor leaves after grinding is 20 mesh.

Please refer to Fig. 1, which is the preparation method of Chinese medicinal material extract of the present invention, which includes the following steps: 1. Extraction step S1: First, extract any one of agarwood, saponin, horn thorn, cinnamon leaves, and camphor leaves. or one of the combination of raw materials and ultrapure water is mixed at a weight ratio of 1:5 to 1:10, wherein the weight ratio of raw materials and ultrapure water is preferably 1:10, after mixing raw materials with ultrapure water, in liquid nitrogen Frozen in a barrel and then thawed at room temperature, and the cycle freeze-thaw treatment is performed to break the cell wall of the raw material, and then the raw material is heated with ultrapure water for extraction to obtain an extract, wherein the extraction temperature is between 90°C and 110°C. The temperature is preferably 100°C, and the extraction time is 1.5 hours to 2.5 hours, preferably 2 hours.

1. Filtration step S2: After the extract is cooled, filter the extract; in this embodiment, filter the extract in 4 steps to obtain the filtered extract, first coarsely filter the extract; then use a centrifuge to Centrifuge the extract after coarse filtration, and collect the supernatant after the centrifugation. The centrifugation speed is 7,000~15,000rpm, and the centrifugation time is 3~10 minutes. Among them, the centrifugation speed is 10,000rpm, and the centrifugation time is 5 minutes. ; Then filter the supernatant with a qualitative filter paper (Whatman NO.4 qualitative filter paper) with a pore size of 15-30 μm; finally filter with a glass fiber filter membrane (MGC glass fiber filter membrane) with a pore size as fine as 1.2 μm, to Obtain the filtered extract.

A drying step S3: After the filtering step, the filtered extract is placed in an environment of -35~-10°C to form a frozen state, and the frozen extract is subjected to vacuum freeze-drying to obtain the Chinese herbal medicine extract, wherein, The extracts of Chinese herbal medicines are freeze-dried powders, which are convenient for preservation and preparation in application.

In this embodiment, the extracts of Chinese herbal medicines can be prepared into aqueous solutions with different concentrations relative to the solvents, wherein the solvents used in the preparation can be water, reverse osmosis water, deionized water, ethanol, methanol, isopropanol or Other suitable and less irritating solvents.

The following discloses the analysis results of the Chinese herbal extracts in the first embodiment of the present invention; in this embodiment, the active ingredients of the Chinese herbal extracts are composed of agarwood, saponins, horn thorns, cinnamon leaves and camphor leaves. The weight ratio of horns, horn spines, cinnamon leaves, and camphor leaves is 1:1:1:1:1; the analysis results include SARS-CoV-2 Spike protein and Human Angiotensin-converting Enzyme 2, The ability of hACE2) to combine with intervention, and the detailed steps and results of SARS-CoV-2 3CL ^protease activity inhibition ability. The numerical bars presented in all analysis results are the mean ± standard deviation of 50 repeated experiments.

1. SARS-CoV-2 full-sequence spike protein binding intervention analysis

The spike protein of SARS-CoV-2 plays an important role in the mechanism of the virus entering human cells. In this example, the Chinese herbal medicine of the present invention is evaluated by an enzyme-linked immunosorbent assay (ELISA). The potential inhibitory effect and intervention ability of the extract on the combination of the full sequence of spike protein of SARS-CoV-2 (Wuhan strain) and hACE2; among them, the hACE2 used in the experiment is an in vitro synthesized protein.

The experimental steps of the enzyme-binding immunosorbent assay method are as follows: First, take 100 μL of spinin solution (500 ng/mL; CAT.GTX135972-pro, GeneTex , Taipei, Taiwan), the echinin solution was injected into a 96-well micro-analysis plate, the micro-analysis plate was sealed and kept at 4°C overnight. Next, use a washing buffer solution composed of 0.05% (v/v) phosphate buffer solution and Tween-20 to wash the 96-well micro-analytical plate that has been injected with spinin solution. After washing three times in total, it will not be able to adhere to the 96-well micro-analysis plate. The echinin solution from the assay plate was removed.

Next, add 300 μL of the ELISA blocking solution prepared by washing buffer solution containing 0.5% (w/v) fetal bovine serum to a 96-well micro-analysis plate, and incubate at 37°C for 1.5 hours, and then wash three times Take 100 μL of Chinese medicinal material extracts of different concentrations (0 to 100000 μg/mL) or 100 μL of inhibitors (10 ug/mL; cat.GTX635791, GeneTex, Taipei, Taiwan) of the present invention and add them to a 96-well microanalysis plate, and Incubate at 37°C for 1 hour and wash 96-well microplates three times with wash buffer.

Then, 100 μL of ELISA signal amplifier Streptavidin-HRP (100 ng/mL; cat. GTX30949, GeneTex, Taipei, Taiwan) was added and incubated at 37° C. for 1 hour.

Finally, 200 μL of TMB chromogenic substrate was added to each well of the 96-well microanalysis plate, and incubated at 37°C for 1 hour in a dark environment. After the reaction is complete, use 50 μL of stop solution to terminate the reaction. And use a microplate reader (Microplate reader) to measure the absorbance value at a wavelength of 450nm.

As shown in Figure 2, it is the result of the intervention of the Chinese medicinal material extract and the inhibitor (RBD antibody) on the binding of SARS-CoV-2 spike protein and hACE2 respectively. Among them, in the case of no addition of hACE2, only the background signal of the SARS-CoV-2 spike protein coating can be measured; the control group is adding hACE2 to the SARS-CoV-2 spike protein coating, and the SARS-CoV-2 spike protein The coating will produce a strong colorimetric signal after adding hACE2, and the binding efficiency is 100%. The experimental group is the intervention effect of adding different concentrations of Chinese herbal extracts on the binding of SARS-CoV-2 spike protein and hACE2.

It can be observed that when the concentration of Chinese herbal extracts exceeds 10,000 μg/mL, the binding efficiency of SARS-CoV-2 spike protein to hACE2 drops below 50%, even when the concentration of Chinese herbal extracts is 100,000 μg/mL, SARS - The binding efficiency of CoV-2 spike protein to hACE2 was reduced to 4%, which was comparable to that of the inhibitor (RBD antibody) in inhibiting SARS-CoV-2 spike protein and hACE2.

Further nonlinear regression analysis is carried out on the binding efficiency of the Chinese medicinal material extract of the present invention to the SARS-CoV-2 spike protein and hACE2, wherein, after 50 experiments, the _half inhibitory concentration IC of the Chinese medicinal material extract of the present invention (Half Maximal Inhibitory Concentration) ranges from 7210 to 10850 μg/mL, and its half-inhibitory concentration IC ₅₀ preferably has an average value of 9024 μg/mL, as shown in Figure 3. The results show that the Chinese herbal medicine extract of the present invention effectively inhibits the binding efficiency of spinin and hACE2 in a dose-dependent manner.

In the present invention, the Chinese herbal medicine extract is further neutralized with the SARS-CoV-2 limited virus. The solvent is deionized water, the concentration of the Chinese herbal medicine extract is 250 mg/mL, and the test is carried out under the test conditions in the following table.

HeLa expressing SmBiT-ACE2 cells, SARS-CoV-2 pseudovirus and different doses of Chinese herbal extracts were co-cultured for 6 hours, and the same amount of PBS solution was used as the control group. After 24 hours of culture, the cells were washed twice with PBS solution , and finally use NanoLuc for bioluminescent detection.

The test results are shown in the table below. The following table shows the neutralization data of Chinese herbal extracts and SARS-CoV-2 limited virus. From the results, it can be seen that the minimum dose of Chinese herbal extracts to block SARS-CoV-2 infected cells is 50 μg/ mL, and the blocking rate was as high as 95%.

In addition, as shown in Figure 4, Figure 4 shows the half-effect concentration EC ₅₀ analysis results of Chinese herbal medicine extracts on the combination of SARS-CoV-2 pseudovirus and HeLa SmBiT-ACE2 expressing cells, wherein the Chinese herbal medicine extracts on SARS-CoV -2 Pseudovirus exhibits dose-dependent neutralizing activity, the half-effect concentration EC ₅₀ (Concentration For 50% of Maximal Effect) value ranges between 20-30 μg/mL, and its half-effect concentration EC ₅₀ better average value is 24.5 μg/mL mL.

2. SARS-CoV-2 3CL ^pro protease activity inhibition analysis

SARS-CoV-2 3CL ^protease is an enzyme involved in the synthesis and replication of viral RNA. In this example, the effect of the Chinese herbal extracts of the present invention on SARS- CoV-2 3CL ^pro protease activity inhibitory ability.

The experimental steps of fluorescence resonance energy transfer technology analysis are as follows: First, DABCYL-TSAVLQSGFRKMG-EDANS (Genomics, Taiwan) modified peptide was dissolved in water and co-cultured with SARS CoV-2 3CL ^pro .

Next, using a multi-function microplate reader (SPARK® multimode microplate reader, TECAN) as the test instrument, the test was carried out at 37°C to ensure the activity of the protein. At the same time, the fluorescence intensity of EDANS when the modified peptide was hydrolyzed was used as a function of time. Wherein, the emission wavelengths are 340nm and 490nm; the bandwidths are 9nm and 15nm respectively.

Next, it was evaluated whether the emission wavelength of the test sample at 340 nm would overlap with the emission spectrum of EDANS before the test. Finally, test the influence of different concentrations (0 to 31.25 μg/mL) of Chinese herbal extracts of the present invention on the activity of SARS-CoV-2 3CL ^pro protease, and use GraphPad Prism 7.03 (GraphPad Software, San Diego, CA, USA) for statistics analyze.

As shown in Figure 5, it is the analysis result of the inhibition of SARS-CoV-2 3CL ^pro protease activity by the Chinese herbal medicine extract of the present invention. It can be observed that after using 0.98 μg/mL of Chinese herbal extracts, the relative activity of SARS-CoV-2 3CL ^pro was reduced to about 80%; after using 7.81 μg/mL of Chinese herbal extracts, the relative activity of SARS-CoV-2 3CL pro The relative activity of ^pro decreased to about 70%. As the concentration of Chinese herbal extracts increased to 15.63-31.25 μg/mL, the relative activity of SARS-CoV-2 3CL ^pro was further inhibited, and its activity dropped to about 60% and 50%, respectively.

After 50 experiments, the half-inhibitory concentration IC ₅₀ of the Chinese herbal medicine extracts of the present invention inhibited the activity of SARS-CoV-2 3CL ^protease ranged from 26 to 40 μg/mL, and the better average value of the half-inhibition concentration IC ₅₀ It is 32.61±4.226μg/mL, as shown in Figure 6, the results show that the Chinese medicinal material extract of the present invention has a dose-dependent ability to reduce the relative activity of SARS-CoV-2 3CL ^pro , showing that the Chinese medicinal material extract of the present invention It has therapeutic potential against SARS-CoV-2.

The following discloses the analysis results of the Chinese herbal extracts in the second embodiment of the present invention; in this embodiment, the active ingredient of the Chinese herbal extracts is cinnamon leaves; the analysis results are the results of the ability to inhibit the activity of SARS-CoV-2 3CL ^protease , The detailed steps are the same as those described in the first embodiment above, and will not be repeated here. The numerical bars presented in all the analysis results are the average values ± standard deviations obtained from 50 repeated experiments.

As shown in Figure 7, after 50 experiments, the half-inhibitory concentration _IC50 value range of the Chinese herbal extracts of the present invention to the inhibition of SARS-CoV-2 3CL ^protease activity is between 10-15 μg/mL, and the half-inhibitory concentration The preferred average value of IC ₅₀ is 13.08±0.73 μg/mL.

It should be noted that in this example, when the concentration of the Chinese herbal medicine extract was 25 μg/mL and 50 μg/mL, it could inhibit the activity of SARS-CoV-2 3CL ^pro protease by 69% and 83%, respectively, while the Chinese herbal medicine When the concentration of the extract is 80 μg/mL, it can inhibit the activity of SARS-CoV-2 3CL ^pro protease by 100%.

It can be seen from the above examples that the cinnamon leaf extract has excellent performance in inhibiting the activity of SARS-CoV-2 3CL ^pro protease. Therefore, the present invention can utilize the pharmacological effects produced by different compositions and different proportions of active ingredients, through single prescription, The deployment of the compound is aimed at the symptoms or diseases related to the coronavirus, and the curative effect is enhanced and extended according to the severity of the symptoms, so as to achieve the best therapeutic effect.

For example, if a patient is in the early stage of suffering from coronavirus, since the amount of virus in the patient's body is still high at this time, a formula with a higher weight ratio of cinnamon leaves can be used to effectively inhibit the increase of the virus and reduce For the overall efficiency of the virus, the weight ratio of cinnamon leaves to agarwood, saponins, horn thorns, and camphor leaves can be 1.5:1:1:1:1, or 2:1:1:1:1, or 2.5 :1:1:1:1, or 3:1:1:1:1, or 3.5:1:1:1:1, or 4:1:1:1:1, or 4.5:1 :1:1:1, or 5:1:1:1:1.

As mentioned above, agarwood has the effects of promoting qi and relieving pain, lowering the reverse and regulating the middle, warming the kidney and inhaling qi, dredging the meridians to calm the mind, sedative and analgesic, etc. The pods have the functions of dispelling stubborn phlegm, opening and closing the orifices, expelling wind and killing insects; Swelling, detoxification, pus drainage, and insecticidal effects; Camphor leaves have the effects of expelling wind, dehumidification, detoxification, and insecticidal. Therefore, a formula with a lower weight ratio of cinnamon leaves and a higher weight ratio of agarwood, saponins, horn thorns, and camphor leaves can be used to improve effects such as analgesia, expectoration, swelling, and dehumidification.

In addition, the Chinese herbal extract of the present invention can be used to prepare a medicament for treating coronavirus-related symptoms or diseases, wherein the medicament includes the Chinese herbal extract of the present invention and a medically acceptable carrier.

According to the present invention, the aforesaid "carrier" refers to a drug carrier commonly used in the medical field, such as fillers, binders, diluents, excipients, acceptors or the like, which are generally skilled in the manufacturing of pharmaceuticals well known.

In this embodiment, the dosage form of the medicament includes mouthwash, oronasal inhalation or oral capsule, but not limited thereto. Among them, by using mouthwash containing Chinese herbal extracts, the residual virus in the mouth and throat can be combined and removed by spitting out the coronavirus; and the use of oral and nasal inhalers containing Chinese herbal extracts can utilize The microvessels of the nasal cavity or lungs absorb the extracts of Chinese herbal medicines to eliminate coronaviruses; while taking oral capsules containing extracts of Chinese herbal medicines, the extracts of Chinese herbal medicines can be directly absorbed and utilized by the human body through the stomach to eliminate coronaviruses.

According to the present invention, the dosage of the medicament prepared from the extract of Chinese medicinal materials can be determined by relevant medical personnel according to the common knowledge in the technical field according to factors such as the patient's age, weight, health status, and disease progression. The medicaments prepared from the aforementioned Chinese herbal extracts can also be administered alone or co-administered with other medicaments, and the course of administration should be carried out according to the routine methods of pharmacy by doctors or relevant persons.

In this way, by taking medicines containing extracts of Chinese medicinal materials, it can effectively interfere with the binding of Spike Protein of SARS-CoV-2 coronavirus to hACE2, and inhibit the activity of 3CL ^{pro protease} of SARS-CoV-2 coronavirus, To achieve the effect of treating coronavirus-related symptoms or diseases.

The above-mentioned embodiments are only used to illustrate the present invention, and are not intended to limit the scope of the present invention. All modifications or changes that do not violate the spirit of the present invention belong to the intended protection category of the present invention.

Claims (14)

An extract of Chinese medicinal materials, which contains an active ingredient consisting of agarwood, a saponin, a thorn, a cinnamon leaf and a camphor leaf. The agarwood, the saponin, the thorn, and the cinnamon leaf And the weight ratio of the camphor leaves is 1~5:1~5:1~5:1~5:1~5. The Chinese medicinal material extract as described in Claim 1, wherein the weight ratio of the agarwood, the saponins, the horn thorns, the cinnamon leaves, and the camphor leaves is 1:1:1:1:1. A preparation method of Chinese medicinal material extract as described in Claim 1, which comprises the following steps: an extraction step: extract any of the agarwood, the saponin, the horn thorn, the cinnamon leaf, and the camphor leaf One or a combination of raw materials and ultrapure water are mixed at a weight ratio of 1:5 to 1:10 and subjected to cycle freeze-thaw treatment, and then heated for extraction to obtain an extract, wherein the extraction temperature is between 90°C and 110°C °C, the extraction time ranges from 1.5 hours to 2.5 hours; a filtering step: filtering the extract; and a drying step: vacuum freeze-drying the extract to obtain the Chinese herbal medicine extract. The preparation method as described in claim 3, wherein, when the raw material is composed of the agarwood, the saponins, the thorns, the cinnamon leaves and the camphor leaves, the agarwood, the saponins, the thorns, and the cinnamon leaves And the weight ratio of the camphor leaves is 1~5:1~5:1~5:1~5:1~5. The preparation method as described in claim 4, wherein the weight ratio of the agarwood, the saponins, the horn thorns, the cinnamon leaves and the camphor leaves is 1:1:1:1:1. A use of the extract of Chinese medicinal materials as described in claim 1 for preparing a medicament for treating symptoms or diseases related to coronavirus, the active ingredient of the extract of Chinese medicinal materials is the cinnamon leaf. A use of an extract of a Chinese medicinal material for preparing a medicament for inhibiting the activity of SARS-CoV-2 3CL ^protease , the active ingredient of the extract of a Chinese medicinal material is cinnamon leaf. The use of Chinese herbal extracts as described in Claim 7, wherein the half-inhibitory concentration IC ₅₀ of the drug ranges from 10 to 15 μg/mL. A use of the Chinese medicinal material extract as described in claim 1 for preparing a medicament for treating symptoms or diseases related to coronavirus. A use of the Chinese medicinal material extract as described in claim 1 for preparing a medicament for interfering with the spike protein of SARS-CoV-2. The use of Chinese herbal extracts as described in Claim 10, wherein the half-effect concentration EC ₅₀ of the drug ranges from 20 to 30 μg/mL. A use of Chinese herbal extracts as described in claim 1 for preparing a medicament for inhibiting the activity of SARS-CoV-2 3CL ^pro protease. The use of Chinese herbal extracts as described in Claim 12, wherein the half-inhibitory concentration IC ₅₀ of the drug ranges from 26 to 40 μg/mL. The use of Chinese herbal extracts as described in any one of claims 6, 7, 9 or 10, wherein the dosage form of the medicament includes mouthwash, oronasal inhalation or oral capsule.

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