TWI796204B - Method of extracting sarcodia, extract of sarcodia and use of extract of sarcodia - Google Patents

Abstract

This invention provides a method of extracting Sarcodia, an extract of Sarcodia and an use of an extract of Sarcodia. The extract is extracted by the extracting method comprising: (a) providing dried Sarcodia powders; (b) extracting the Sarcodia powders by liquid carbon dioxide to obtain the extracting liquid of Sarcodia, wherein the extracting liquid of Sarcodia contains the extract of Sarcodia and the liquid carbon dioxide solvent, the extracting pressure is 300-650 bar, the extracting temperature is 45-55℃, the extracting time is 2-6 hours; and (c) separating the extract of Sarcodia from the extracting liquid of Sarcodia, the separating pressure is 30-100 bar. The extract of Sarcodia is used to manufacture drugs or cosmetics for accelerating the growth activity of hair follicle cells, inhibiting Propionibacterium acnes growth or accelerating the activity of keratinocytes. By the above extract of Sarcodia, a new use with higher commercial value is developed.

Description

Extraction method of sea fungus, sea fungus extract and use thereof

The present invention relates to a sea fungus extraction method, a sea fungus extract and uses thereof, especially a sea fungus extract obtained through supercritical fluid extraction.

Sea fungus ( Sarcodia suiae ) is a dark red flat algal body. It is known that sea fungus contains rich nutrients, including dietary fiber, minerals, essential amino acids, vitamin E and Omega fatty acids. Therefore, sea fungus is regarded as a healthy food that is beneficial to human health.

However, except that sea fungus is known to be rich in nutrients and is suitable as a healthy food, no components with high economic value have been extracted from sea fungus. Therefore, how to provide a new extraction method to extract components with high economic value from sea fungus is still an issue to be solved.

The purpose of the present invention is to address the above problems, and to provide an extraction method of sea fungus extract, which comprises the following steps: (a) providing dry sea fungus powder; Dried sea fungus powder is extracted to obtain sea fungus extract, which contains sea fungus extract and the carbon dioxide fluid solvent; wherein, the extraction pressure is 300-650bar, the extraction temperature is 45-55°C, and the extraction time is 2 -6 hours; and (c) separating the sea fungus extract from the sea fungus extract, wherein the separation pressure is 30-100 bar.

As mentioned above, the extraction pressure is 350 bar, the extraction temperature is 55° C., and the extraction time is 2 hours.

As mentioned above, the extraction pressure was 365 bar, the extraction temperature was 55° C., and the extraction time was 4 hours.

As mentioned above, the extraction pressure was 420 bar, the extraction temperature was 55° C., and the extraction time was 4 hours.

In order to achieve the above and other purposes, the present invention provides a sea fungus extract, which is obtained by the above-mentioned extraction method.

In order to achieve the above purpose and other purposes, the present invention provides a use of the above-mentioned sea fungus extract for preparing a drug for promoting hair follicle cell growth activity, and the concentration of the sea fungus extract is above 5 μg/mL.

In order to achieve the above purpose and other purposes, the present invention provides a use of the above-mentioned sea fungus extract for the preparation of medicines or cosmetic products for inhibiting the growth of acne bacteria, and the concentration of the sea fungus extract is above 0.5 mg/mL.

In order to achieve the above purpose and other purposes, the present invention provides a use of the above-mentioned sea fungus extract for preparing a drug or cosmetic product for enhancing keratinocyte migration activity, and the concentration of the sea fungus extract is above 1 μg/mL.

In order to achieve the above purpose and other purposes, the present invention provides a use of the above-mentioned sea fungus extract in the preparation of drugs or cosmetic products that enhance the growth activity of keratinocytes, and the concentration of the sea fungus extract is above 6 μg/mL.

With the above-mentioned sea fungus extract, its extraction method and its use, a new extraction method can be provided to extract components with high economic value from sea fungus.

Fig. 1 shows the HPLC fingerprint of the sea fungus extract of Example 1 of the present invention under UV light with a wavelength of 210nm.

Fig. 2 shows the HPLC fingerprint of the sea fungus extract of Example 1 of the present invention under UV light with a wavelength of 254nm.

Fig. 3 shows the HPLC fingerprint of the sea fungus extract of Example 1 of the present invention under UV light with a wavelength of 280nm.

Fig. 4 shows the gas chromatogram of the sea fungus extract of Example 1 of the present invention.

Fig. 5 shows the results of the cell growth promoting test of the sea fungus extract in Example 1 of the present invention on human dermal papilla cells.

Fig. 6 shows the results of the cell growth promotion test of the sea fungus extract of Example 2 of the present invention on human dermal papilla cells.

Fig. 7 shows the results of the cell growth promoting test of the sea fungus extract in Example 3 of the present invention on human dermal papilla cells.

Fig. 8 shows the comparison results of HPLC fingerprints of different sea fungus extracts in Example 4 of the present invention.

Fig. 9 shows the fluorescence image of the test of the ability to promote keratinocyte migration of the sea fungus extract of Example 6 of the present invention.

Fig. 10 shows the cell migration rate of the sea fungus extract of Example 6 of the present invention in promoting the migration ability of keratinocytes.

Fig. 11 shows the results of the keratinocyte growth-promoting test of the sea fungus extract in Example 7 of the present invention.

In order to fully understand the purpose, features and effects of the present invention, the present invention will be described in detail through the following specific embodiments and accompanying drawings, as follows:

The extraction method of the sea fungus extract of embodiment 1

The extraction method of the sea fungus extract in Example 1 is summarized as follows.

First, dry sea fungus powder is provided. The dried sea fungus powder in Example 1 is prepared by the general preparation method of sea fungus powder, but it can also be obtained through commercial purchase. The general preparation method of sea fungus powder is as follows: clean the fresh sea fungus after harvesting, and dry the sea fungus. The drying method is to place the sea fungus in an oven at 50°C for 24 hours. The sea fungus is crushed into powder with a pulverizer to prepare sea fungus powder. The particle size of the sea fungus powder is preferably 1-4mm, but the particle size of the sea fungus powder can still be adjusted according to other requirements.

Next, prepare supercritical fluid extraction equipment, the aforementioned supercritical fluid extraction equipment is provided by the Metal Industry Research and Development Center (MIRDC), and the model is SFE-350S-1000R. The sea fungus extract is extracted from the aforementioned sea fungus powder using supercritical fluid extraction equipment. The extraction method is as follows, the aforementioned sea fungus powder is placed in the extraction tank of the supercritical fluid extraction equipment, the use of sea fungus powder The amount is about 90-99% of the volume of the extraction tank body (the feed volume of sea fungus powder is 850g to 950g, and the volume of the extraction tank body is 1.2L). Then, using supercritical fluid as a solvent, extract the dry sea fungus powder according to the operation method provided in the operation manual of the supercritical fluid extraction equipment. The supercritical fluid used in this embodiment 1 is carbon dioxide fluid, extract The extraction pressure in the tank is 350bar (adjustable between 300bar-650bar according to the process requirements), the extraction temperature in the extraction tank is 55°C (the extraction temperature can be adjusted between 45°C-55°C according to the process requirements), and the extraction time is 2 Hours, the flow rate of the carbon dioxide fluid is set to 10L/h, but the flow rate of the carbon dioxide fluid can be set in the range of 5~15L/h depending on the extraction process requirements. After the extraction is completed, the sea fungus extract can be obtained. The sea fungus extract contains the sea fungus extract and the carbon dioxide fluid solvent, and the sea fungus extract is dissolved in the carbon dioxide fluid solvent.

Finally, send the sea fungus extract into the separation tank of the supercritical fluid extraction device for decompression, the separation pressure is 35bar (the separation pressure can be adjusted between 30-100bar based on the separation requirements of the sea fungus extract), by In this way, the sea fungus extract can be separated from the sea fungus extract. After the sea fungus extract is separated from the carbon dioxide fluid solvent, the extraction pipeline of the supercritical fluid extraction equipment is washed with ethanol, and the The sea fungus extract is washed out, that is, the sea fungus extract is dissolved in ethanol, and the ethanol in which the sea fungus extract is dissolved flows out from the extraction pipeline, thereby collecting the sea fungus extract The sea fungus extract dissolved in ethanol is placed at room temperature, and the sea fungus extract can be obtained after the ethanol is completely volatilized at room temperature.

The HPLC analysis of the sea fungus extract of embodiment 1

In order to analyze the components in the sea fungus extract of Example 1, the HPLC column was used for analysis, and the analysis conditions were as follows. Use HPLC column Poroshell HPH-C188 (4.6 x 100mm, 2.7μm), flow rate is 1ml/min, and use ultraviolet/visible light detector Agilent 1260 DAD to detect Detection (refer to the operation manual of Agilent 1260 DAD for the detection process), and the detection light is set to UV light with wavelengths of 210nm, 254nm and 280nm. The HPLC fingerprints of sea fungus extract are shown in Figure 1-3.

The GC/MS analysis of the sea fungus extract of embodiment 1

The above-mentioned sea fungus extract was analyzed by gas chromatography (GC) (Agilent 7890B GC/5977B EI mass spectrometer detector). In order to obtain a clearer GC spectrum, the above-mentioned sea fungus extract sample was also taken before silanization The gas chromatography analysis was carried out in the same manner. The analysis column used in the above gas chromatography was ZB-1XT SIMDIST column (5m x 0.53mm ID, 0.09μm film thickness), and the analysis results of the gas chromatography were as follows As shown in Figure 4, the sea fungus extract contains fatty acids such as myristic acid, palmitic acid, stearic acid and phytosterols.

The test of promoting cell growth of human hair follicle dermal papilla cells by sea fungus extract of embodiment 1

First, the sea fungus extract was prepared by the above extraction method; at the same time, the human Follicle Dermal Papilla Cell (Follicle Dermal Papilla Cell) cell line HFDPC (purchased from PromoCell Company, product model C12071) was prepared.

Next, prepare a 96-well cell culture dish, and add cell solution containing HFDPC cells (Follicle Dermal Papilla Cell Growth Medium (PromoCell, C-26501)) to 9 wells, and each well contains 5-8 ×10 ³ cells, with three wells as a group, divide the 9 wells added with HFDPC cell solution into control group, experimental group 1 and experimental group 2, control group did not add sea fungus extract at all, experimental group 1 added final The sea fungus extract with a concentration of 5 μg/mL was added to the experimental group 2 with a final concentration of 25 μg/mL sea fungus extract. After the sea fungus extract was added to experimental group 1 and experimental group 2, the cell culture plate was placed in a cell culture incubator at 37°C for 48 hours, and at the 24th hour and 48th hour, respectively, from Samples were taken from each well, and the cell growth rate of experimental group 1 and experimental group 2 was tested by cell viability test (MTT test). The calculation method was (absorbance value of the experimental group (1 or 2)/absorbance value of the control group) x 100.

The results of the test are shown in Table 1 and Figure 5 below. When the experimental group 1 was cultured to the 24th hour, its cell growth rate relative to the control group was 106.5%. The cell growth rate was 108.3%; the experimental group 2 was cultured to the 24th hour, and its cell growth rate relative to the control group was 113.1%, and the experimental group 2 was cultivated to the 48th hour, and its cell growth rate relative to the control group was 109.2%.

Cell growth promotion test of sea fungus extract in embodiment 2 on human hair follicle dermal papilla cells

First, according to the sea fungus extraction method of embodiment 1 but the extraction pressure is adjusted to 365bar to prepare the sea fungus extract of embodiment 2; meanwhile, prepare the cell line HFDPC of human hair follicle dermal papilla cells (purchased from PromoCell company, product model for C12071).

Next, prepare the cell culture plate, and add cell solution containing HFDPC cells to 9 wells, and use three wells as a group, divide the 9 wells added with HFDPC cell solution into control group, experimental group 1 and experimental group 2, control group The sea fungus extract was not added at all, the sea fungus extract was added to the experimental group 1 with a final concentration of 5 μg/mL, and the sea fungus extract was added to the experimental group 2 with a final concentration of 25 μg/mL. After the sea fungus extract was added to experimental group 1 and experimental group 2, the cell culture plate was placed in a cell culture incubator at 37°C for 48 hours, and at the 24th hour and 48th hour, respectively, from Samples were taken from each well, and the growth rate of cells in each group was tested by MTT assay.

The test results are shown in Figure 6. The experimental group 1 had a cell growth rate of 107.2% relative to the control group when it was cultured to the 24th hour, and the cell growth rate of the experimental group 1 was 107.2% compared to the control group when it was cultured to the 48th hour. It was 111.6%; the growth rate of cells in experimental group 2 was 125.7% compared to the control group at the 24th hour of cultivation, and the cell growth rate of experimental group 2 was 145.9% compared with the control group at the 48th hour of cultivation.

Cell growth promotion test of sea fungus extract in embodiment 3 on human hair follicle dermal papilla cells

First, according to the sea fungus extraction method of embodiment 1 but the extraction pressure is adjusted to 420bar to prepare the sea fungus extract of embodiment 3; meanwhile, prepare the cell line HFDPC of human hair follicle dermal papilla cells (purchased from PromoCell company, product model for C12071).

Next, prepare the cell culture plate, and add cell solution containing HFDPC cells to 9 wells, and use three wells as a group, divide the 9 wells added with HFDPC cell solution into control group, experimental group 1 and experimental group 2, control group The sea fungus extract was not added at all, the sea fungus extract was added to the experimental group 1 with a final concentration of 5 μg/mL, and the sea fungus extract was added to the experimental group 2 with a final concentration of 25 μg/mL. After the sea fungus extract was added to experimental group 1 and experimental group 2, the cell culture plate was placed in a cell culture incubator at 37°C for 48 hours, and at the 24th hour and 48th hour, respectively, from Samples were taken from each well, and the growth rate of cells in each group was tested by MTT assay.

The test results are shown in Figure 7. The experimental group 1 had a cell growth rate of 105.6% relative to the control group when it was cultured to the 24th hour, and the cell growth rate of the experimental group 1 was 105.6% compared to the control group when it was cultured to the 48th hour. It was 110.3%; the experimental group 2 was cultured to the 24th hour, and its cell growth rate relative to the control group was 116.2%, and the experimental group 2 was cultured to the 48th hour, and its cell growth rate relative to the control group was 126.1%.

The HPLC fingerprint spectrum comparison of the different sea fungus extracts of embodiment 4

First, sea fungus extracts A-E are provided as comparative samples.

Sea fungus extract A is prepared according to the extraction method of sea fungus extract in the aforementioned embodiment 1, and the extraction conditions are set as the following conditions: the extraction solvent is carbon dioxide fluid, the extraction pressure in the extraction tank is 350bar, and the The extraction temperature is 55°C and the extraction time is 6 hours.

Sea fungus extract B is prepared according to the extraction method of the sea fungus extract in the aforementioned embodiment 1, and the extraction conditions are set as the following conditions: the extraction solvent is carbon dioxide fluid, the extraction pressure in the extraction tank is 650bar, and the The extraction temperature is 55°C, and the extraction time is 2 hours.

The sea fungus extract C is to extract the sea fungus extract with ethyl acetate, the extraction temperature is 50°C, the extraction pressure is 1 bar, and the extraction time is 6 hours.

Sea fungus extract D uses pure water to extract the sea fungus extract, the extraction temperature is 50°C, the extraction pressure is 1 bar, and the extraction time is 6 hours.

Sea fungus extract E extracts sea fungus extract with 95% ethanol, the extraction temperature is 50°C, the extraction pressure is 1 bar, and the extraction time is 6 hours.

Next, the above-mentioned sea fungus extracts A-E were subjected to HPLC analysis, and the analysis conditions were as follows. An HPLC column TSKgel ODS-100S (250 x 4.6mm) was used with a flow rate of 1ml/min, and an ultraviolet/visible light detector was used for detection, and the detection light was set to UV light with a wavelength of 280nm.

The analysis results are shown in Figure 8. Judging from the peaks of the HPLC fingerprints of various sea fungus extracts in the figure, the content components of sea fungus extracts A and B extracted with the same supercritical fluid but at different extraction pressures are relatively high. Similarly, the content components of sea fungus extracts C-E extracted with ethyl acetate, water and ethanol as solvents are different from those of sea fungus extracts A and B.

The test of inhibiting the growth of acne bacteria of the sea fungus extract of embodiment 5

First, prepare the sea fungus extract according to the sea fungus extraction method of Example 1; meanwhile, prepare acne bacteria as the test target of the antibacterial effect of the sea fungus extract, and the selected acne bacteria species is Propionibacterium acnes ( Propionibacterium acnes ) (BCRC 10723). And, prepare four trays of enhanced Clostridium medium (RCM medium), the first medium contains the antibiotic gentamycin (Gentamycin) with a concentration of 50 μg/mL as control group 1; the second medium does not add additional components as Control group 2; adding the above-mentioned sea fungus extract at a concentration of 1 mg/mL to the third medium as experimental group 1; adding the above-mentioned sea fungus extract at a concentration of 0.5 mg/mL to the fourth medium as experimental group 2.

Next, the acne bacteria were inoculated in the enhanced Clostridium liquid medium (RCM) culture medium, the initial inoculum amount of the acne bacteria was 1.15×10 ⁵ CFU/mL, and after anaerobic culture at 37°C for 48 hours, the acne bacteria Acne bacteria solution, and evenly smear 100 μL of acne bacteria solution on the above four groups of culture medium, and then put the above four groups of acne bacteria culture medium on the 37 ° C incubator for anaerobic culture for 48 hours, after the cultivation is completed, remove from the incubator The above-mentioned four groups of medium were taken out, and the colony-forming units (CFU) of the above-mentioned four groups of medium were calculated.

The test results of Example 5 are shown in Table 2 below. Because of the addition of antibiotics in the control group 1, the bactericidal effect can be achieved. Compared with the phenomenon of inhibiting the growth of bacteria, when the concentration of the sea fungus extract in Example 5 reaches 1 mg/mL, it has a bactericidal effect.

The test of the ability to promote keratinocyte movement of the sea fungus extract of embodiment 6

First, sea fungus extracts F-H are provided as comparative samples.

The sea fungus extract F is prepared according to the extraction method of the sea fungus extract in the aforementioned Example 1, and the extraction conditions are set as the following conditions: the extraction object is dry sea fungus fine powder numbered 1081204, the extraction solvent is carbon dioxide fluid, The extraction pressure in the extraction tank was 365 bar, the extraction temperature in the extraction tank was 55° C., and the extraction time was 4 hours.

Sea fungus extract G is prepared according to the extraction method of the sea fungus extract in the aforementioned Example 1, and the extraction conditions are set as the following conditions: the extraction object is dry sea fungus fine powder numbered 1081105-1, and the extraction solvent is carbon dioxide fluid, the extraction pressure in the extraction tank is 365 bar, the extraction temperature in the extraction tank is 55°C, and the extraction time is 4 hours.

The sea fungus extract H is prepared according to the extraction method of the sea fungus extract in the aforementioned Example 1, and the extraction conditions are set as the following conditions: the extraction object is dry sea fungus fine powder of No. 1081105-2, and the extraction solvent is carbon dioxide fluid, the extraction pressure in the extraction tank is 420 bar, the extraction temperature in the extraction tank is 55° C., and the extraction time is 4 hours.

Next, prepare a 96-well cell culture plate and the Oris ^™ Cell Migration set, insert the stoppers in the Oris ^™ Cell Migration set into 15 wells, and put the stoppers into the above 15 holes Then, inoculate 2× ¹⁰⁴ human keratinocyte strains (ATCC CRL-2309) in the above-mentioned wells respectively, and use three wells as a group, and divide the above-mentioned 15 wells inoculated with human keratinocyte strains into control group 1, Control group 2, experimental group 1, experimental group 2 and experimental group 3. Add 0.05% DMSO to the control group 1; add 0.05% DMSO and 100ng/mL epidermal growth factor (EGF) to the control group 2 (EGF can promote the movement of human keratinocytes); add 0.05% DMSO and 1 μg/mL sea fungus extract F; 0.05% DMSO and 1 μg/mL sea fungus extract G were added to experimental group 2; 0.05% DMSO and 1 μg/mL sea fungus extract H were added to experimental group 3. After adding the test samples to the above five groups, put the above 96-well cell culture dish into the cell culture incubator and cultivate it in the environment of 37°C for 24 hours. Remove the supernatant in each group of wells, then add 50 μL of vital fluorescent dye (Calcein AM) (1 μM) and let stand for 1 hour.

Finally, use a fluorescent microscope Olympus CKX53 to take images of the above five groups of wells, and calculate the difference between the human keratinocyte lines in the above five groups of wells from the reading values of the captured images (that is, the reading values of the circled fluorescent signals). cell mobility.

The test results are shown in Figures 9 and 10. It can be seen from Figure 9 that compared with the control group 1 that does not add ingredients that can promote the movement of human keratinocytes, the experimental group 1, the experimental group 2 and the experimental group 3 have obvious cell migration trends, and it can be seen from Figure 10 that the experimental group 1. The cell migration rates of the experimental group 2 and the experimental group 3 were higher than those of the control group 1, the cell migration rate of the control group 1 was 100%, the cell migration rate of the experimental group 1 was 165.78%, and the cell migration rate of the experimental group 2 was 190.25%. The mobility rate of experimental group 3 was 156.08%.

The growth-promoting keratinocyte growth test of the sea fungus extract of embodiment 7

First, the sea fungus extract was prepared according to the sea fungus extraction method in Example 1; at the same time, a human keratinocyte cell line (ATCC CRL-2309) was prepared as the test object of this experiment.

Next, prepare a 96-well cell culture plate, inoculate 2×10 ⁴ human keratinocytes (ATCC CRL-2309) in 15 wells, and use three wells as a group, and inoculate the above-mentioned cells inoculated with the human keratinocytes The 15 wells were divided into control group 1, control group 2, experimental group 1, experimental group 2 and experimental group 3. Add 1% DMSO to control group 1; add 1% DMSO and 100ng/mL epidermal growth factor (EGF) to control group 2; add 1% DMSO and 1.2μg/mL sea fungus extract to experimental group 1 ; 1% DMSO and 6.0 μg/mL sea fungus extract were added to experimental group 2; 1% DMSO and 24.1 μg/mL sea fungus extract were added to experimental group 3.

After adding the test samples to the above five groups, put the above 96-well cell culture plate into the cell culture incubator and cultivate it at 37°C for 24 hours. cell viability. The general flow of the MTS/PMS test in Example 7 is as follows: add the MTS/PMS reagent to a 96-well cell culture dish and react for 4 hours, measure the absorbance at a wavelength of 490nm and calculate the cell viability

The test results are shown in Figure 11, the sea fungus extract with a concentration above 6 μg/mL has the effect of promoting the growth of keratinocytes. The OD ₄₉₀ absorbance value of control group 1 reached 1.57, the OD ₄₉₀ absorbance value of control group 2 reached 1.62, the OD ₄₉₀ absorbance value of experimental group 1 reached 1.59, the OD ₄₉₀ absorbance value of experimental group 2 reached 1.68, and the OD 490 absorbance value of experimental group 3 reached _1.62. The absorbance value reached 1.89. From the above values, it can be seen that the experimental group 2 (containing 6.0 μg/mL sea fungus extract) has the same effect as the control group 2 (adding epidermal growth factor).

Through the above method of sea fungus extract, a new extraction method is provided to extract components with high economic value from sea fungus, that is, the sea fungus extract extracted by the above method can be used to prepare drugs that promote the growth of hair follicle cells , Drugs or cosmetic products that inhibit the growth of acne bacteria or drugs or cosmetic products that increase the activity of keratinocytes.

The present invention has been disclosed above with preferred embodiments, but those skilled in the art should understand that the embodiments are only used to describe the present invention, and should not be construed as limiting the scope of the present invention. It should be noted that all changes and substitutions equivalent to the embodiment should be included in the scope of the present invention. Therefore, the scope of protection of the present invention should be defined by the scope of the patent application.

Claims (9)

An extraction method of sea fungus extract, which comprises the following steps: (a) providing dry sea fungus ( Sarcodia suiae ) powder; (b) extracting the dry sea fungus powder with carbon dioxide fluid to obtain sea fungus extract, the sea fungus The extract contains sea fungus extract and the carbon dioxide fluid solvent; wherein, the extraction pressure is 300-650 bar, the extraction temperature is 45-55°C, and the extraction time is 2-6 hours; and (c) extracting from the sea fungus extract The sea fungus extract is separated, wherein the separation pressure is 30-100 bar. The method as described in Claim 1, wherein the extraction pressure is 350 bar, the extraction temperature is 55° C., and the extraction time is 2 hours. The method as described in Claim 1, wherein the extraction pressure is 365 bar, the extraction temperature is 55° C., and the extraction time is 4 hours. The method as described in Claim 1, wherein the extraction pressure is 420 bar, the extraction temperature is 55° C., and the extraction time is 4 hours. A sea fungus ( Sarcodia suiae ) extract obtained by the extraction method described in any one of claims 1 to 4. A use of the sea fungus extract as described in claim 5 in the preparation of a drug for promoting the growth of hair follicle cells, the concentration of the sea fungus extract being above 5 μg/mL. A use of the sea fungus extract as described in claim 5 in the preparation of medicines or cosmetic products for inhibiting the growth of acne bacteria, the concentration of the sea fungus extract being above 0.5 mg/mL. A use of the sea fungus extract as described in claim 5 in the preparation of medicines or cosmetic products for enhancing keratinocyte migration activity, the concentration of the sea fungus extract being above 1 μg/mL. A use of the sea fungus extract as described in Claim 5 in the preparation of drugs or cosmetic products for enhancing the growth activity of keratinocytes, the concentration of the sea fungus extract being above 6 μg/mL.

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