TWI753619B - A natural disinfectant formulated with edible fungi and plant extracts - Google Patents
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Abstract
本發明之一種利用可食用之菌類和植物萃取液所調配而成之天然消毒劑，其特徵在於：該消毒劑係具有複數菌類萃取液、複數菜葉類萃取液、複數果樹類萃取液和複數棕櫚類萃取液，且混合比例係為該等菌類萃取液1%~3%，該等菜葉類萃取液2%~4%，該等果樹類萃取液5%~8%，該等棕櫚類萃取液8%~12%。其餘則為溶劑。如此一來，透過上述萃取液所調合而成的消毒劑，由於萃取液皆為可食用之菌類及植物製成且製備過程中並無摻入化學藥品，因此所製成之消毒劑就具有天然無毒環保且對人類及環境無害之功效，而可以大面積地噴灑而不需擔心會產生任何後遺症。 A natural disinfectant prepared by using edible fungi and plant extracts of the present invention is characterized in that: the disinfectant has a plurality of fungi extracts, a plurality of vegetable leaf extracts, a plurality of fruit tree extracts and a plurality of Palm extracts, and the mixing ratio is 1%~3% of these fungi extracts, 2%~4% of these vegetable leaf extracts, 5%~8% of these fruit tree extracts, and these palms Extraction 8%~12%. The rest are solvents. In this way, the disinfectant prepared by the above-mentioned extract is made of edible fungi and plants and no chemicals are mixed in the preparation process. Therefore, the disinfectant produced has natural properties. It is non-toxic, environmentally friendly and harmless to humans and the environment, and can be sprayed on a large area without worrying about any sequelae.
Description
本發明係屬於消毒液之領域，尤其是一種利用多種不同且可食用之菌類和植物的萃取液所調配而成之天然消毒劑。 The present invention belongs to the field of disinfectant, in particular to a natural disinfectant prepared by using the extracts of various edible fungi and plants.
按，醫院常用的低階消毒劑(Low-level disinfectant)包括酒精(Alcohol)、氯氣(Chlorine)、改良式過氧化氫(improved hydrogen peroxide)、優碘(Iodophors)、酚類(phenolics)、含氯化合物(chlorine-containing compound)或季銨鹽化合物(quaternary ammonium compounds；QAC)，通常都會在美國環保局(Environmental Protection Agency；EPA)註冊。這些化學消毒劑各有優缺點，例如酒精可殺結核菌、真菌、病毒，簡單使用、快速反應，但因易燃故不宜大面積噴灑。氯氣可殺結核菌、真菌、病毒、孢子，且便宜、低毒害，但只要5%就會引起眼睛刺激，口咽、食道和胃灼傷，且會產生三鹵甲烷(trihalomethane)的潛在危害。改良式過氧化氫可殺菌、病毒與分枝桿菌，但價格較昂貴。優碘可殺菌、病毒與分枝桿菌及用於血液培養瓶消毒，但需長時間接觸以殺死真菌，且會損壞矽膠導管，比起消毒劑更常做為防腐劑之用。酚類可殺結核菌、真菌、病毒且便宜，但不殺孢子，且被多孔物質(porous materials)吸收時會刺激組織(irritate tissue)。含氯化合物可殺菌、真菌、包膜病毒(如HIV)，但對非包膜病毒、結核菌卻不具殺菌性，且受硬水及棉/紗布影響會降低殺菌力。 According to the press, the low-level disinfectants commonly used in hospitals include alcohol, chlorine, improved hydrogen peroxide, Iodophors, phenolics, containing Chlorine-containing compounds (chlorine-containing compounds) or quaternary ammonium compounds (quaternary ammonium compounds; QAC) are usually registered with the US Environmental Protection Agency (EPA). These chemical disinfectants have their own advantages and disadvantages. For example, alcohol can kill tuberculosis bacteria, fungi, and viruses. It is easy to use and responds quickly. However, it is not suitable for large-scale spraying due to its flammability. Chlorine can kill tuberculosis, fungi, viruses, spores, and is cheap and low-toxic, but only 5% can cause eye irritation, oropharyngeal, esophagus, and heartburn, and potentially produce trihalomethanes. Modified hydrogen peroxide kills bacteria, viruses and mycobacteria, but is more expensive. Betadine kills bacteria, viruses and mycobacteria, and is used to sterilize blood culture bottles, but requires prolonged contact to kill fungi, can damage silicone catheters, and is more commonly used as a preservative than disinfectants. Phenols kill tuberculosis, fungi, viruses and are inexpensive, but are not sporicidal and can irritate tissue when absorbed by porous materials. Chlorine-containing compounds can kill bacteria, fungi, and enveloped viruses (such as HIV), but not non-enveloped viruses and tuberculosis, and are affected by hard water and cotton/gauze.
除此之外，當所使用消毒劑如氯、二氧化氯、臭氧或氯胺(chlorine,chlorine dioxide,ozone,orchloramines)與天然存在的有機物、人為污染物、溴化物和碘化物反應時，就會形成消毒副產物(Disinfection by-products；DBPs)。Richardson,Susan D.,et al.回顧近30年的研究，探討85種DBP的發生、遺傳毒性與致癌性，結果發現：與傳統氯化(氯、二氧化氯)相比，替代性消毒劑(臭氧、氯胺)會增加許多新興的DBP含量，且這些新興DBP的遺傳毒性，要比某些受管制的DBP更高；通常溴化DBP的毒性和致癌性，皆比氯化的化合物為高，而碘化DBP的毒性最大。 In addition to this, when disinfectants such as chlorine, chlorine dioxide, ozone, or chloramines are used, they react with naturally occurring organics, man-made pollutants, bromides and iodides. Disinfection by-products (DBPs) will be formed. Richardson, Susan D., et al. reviewed nearly 30 years of research on the occurrence, genotoxicity, and carcinogenicity of 85 DBPs and found that, compared with traditional chlorination (chlorine, chlorine dioxide), alternative disinfectants (Ozone, chloramines) will increase the content of many emerging DBPs, and these emerging DBPs are more genotoxic than some regulated DBPs; brominated DBPs are generally more toxic and carcinogenic than chlorinated compounds. high, while iodinated DBP is the most toxic.
然而，醫院常用的消毒劑還是如Alcohol,Chlorine,improved hydrogen peroxide(hydrogen peroxide and silver compound),Iodophors,phenolics,chlorine-containing compound(ClO2),quaternary ammonium compounds(QAC),glutaraldehyde,hypochlorite(sodium hypochlorite),......等消毒劑，這些基本上皆屬化學製劑，對人體或環境或多或少都可能存在不同程度的影響或傷害，且在醫院內眾人群聚時，這些消毒劑多數不適合做大面積噴灑。此外，過去發生在醫院中因群聚造成空氣品質「細菌濃度超過管制標準」的事件時有所聞，但非常遺憾的是，研究「醫院室內環境大面積消毒」的論述甚少，若能有一種天然、環保並對人體無害且能大面積噴灑的消毒劑，將可能對醫療院所場域之消毒帶來革命性的影響。 However, the commonly used disinfectants in hospitals are still such as Alcohol, Chlorine, improved hydrogen peroxide (hydrogen peroxide and silver compound), Iodophors, phenolics, chlorine-containing compound (ClO2), quaternary ammonium compounds (QAC), glutaraldehyde, hypochlorite (sodium hypochlorite) ,... and other disinfectants, these are basically chemical preparations, which may have different degrees of impact or harm on the human body or the environment, and when crowds gather in the hospital, most of these disinfectants Not suitable for large area spraying. In addition, incidents of "bacteria concentration exceeding the control standard" in the air quality caused by clustering in hospitals have been heard in the past, but unfortunately, there are very few discussions on "large-scale disinfection of the indoor environment of hospitals". A natural, environmentally friendly and harmless disinfectant that can be sprayed on a large area may have a revolutionary impact on the disinfection of medical institutions.
有鑑於此，本發明人感其未臻完善而竭其心智苦心研究，並憑其從事該項產業多年之累積經驗，進而提供一種利用可食用之菌類和植物萃取液所調配而成之天然消毒劑，以期可以改善上述習知技術之缺失。 In view of this, the inventor of the present invention feels that it is not perfect and exhausts his mental and painstaking research, and relying on his accumulated experience in the industry for many years, and then provide a natural disinfection using edible fungi and plant extracts. In order to improve the above-mentioned deficiencies of the prior art.
本發明之一目的，旨在提供一種利用可食用之菌類和植物萃取液所調配而成之天然消毒劑，以利用多種可食用菌類、蔬果植物的萃取液調合而成，讓製成之消毒劑具有環保、對人體無害且又能大面積噴灑之效果。 One of the objectives of the present invention is to provide a natural disinfectant prepared by using edible fungi and plant extracts. It is environmentally friendly, harmless to the human body and can be sprayed on a large area.
為達上述目的，本發明之一種利用可食用之菌類和植物萃取液所調配而成之天然消毒劑，其特徵在於：該消毒劑係具有複數菌類萃取液、複數菜葉類萃取液、複數果樹類萃取液和複數棕櫚類萃取液，且混合比例係為該等菌類萃取液1%~3%，該等菜葉類萃取液2%~4%，該等果樹類萃取液5%~8%，該等棕櫚類萃取液8%~12%。其餘則為溶劑。 In order to achieve the above-mentioned purpose, a natural disinfectant prepared by utilizing edible fungi and plant extracts of the present invention is characterized in that: the disinfectant has a plurality of fungi extracts, a plurality of vegetable leaf extracts, and a plurality of fruit trees. 1% to 3% of these fungi extracts, 2% to 4% of these vegetable leaf extracts, and 5% to 8% of these fruit tree extracts , 8% to 12% of these palm extracts. The rest are solvents.
較佳者，該等菌類萃取液係由雙孢蘑菇(Agaricus bisporus)、四孢蘑菇(Agaricus campestris)、兄弟牛肝菌(Boletus fraternus)、褐絨柄牛肝菌(Boletus subvelutipes)、鉛色圓孢牛肝菌(Gyrodon lividus)、褐疣柄牛肝菌(Leccinum scabrum)、厚環乳牛肝菌(Suillus grevillei)、美味羊肚菌(Morchella esculenta)、黑紅菇(Russula nigricans)、簇生黃韌傘(Hypholoma sublateritium)和西口孢離褶傘(Lyophyllum sykosporum)所萃取而得。 Preferably, the fungi extracts are made of Agaricus bisporus, Agaricus campestris, Boletus fraternus, Boletus subvelutipes, Plumbago Gyrodon lividus, Leccinum scabrum, Suillus grevillei, Morchella esculenta, Russula nigricans, Flavum fascicosa It is extracted from Hypholoma sublateritium and Lyophyllum sykosporum.
較佳者，該等菜葉類萃取液係由菠菜(Spinacia oleracea)、萵苣(Lactuca sativa)、荷蘭芹(Petroselinum crispum)、羅勒(Ocimum basilicum)、牛蒡(Arctium lappa)、薄荷(Mentha spicata)、紫蘇(Perilla frutescens var.crispa)和大葉冬青(Ilex latifolia)所萃取而得。 Preferably, the vegetable leaf extracts are made of spinach (Spinacia oleracea), lettuce (Lactuca sativa), parsley (Petroselinum crispum), basil (Ocimum basilicum), burdock (Arctium lappa), mint (Mentha spicata), Extracted from Perilla frutescens var.crispa and Ilex latifolia.
較佳者，該等果樹類萃取液係由蘋果(Malus pumila)、西洋梨(Pyrus communis)、檸檬(Citrus limon)、柚(Citrus maxima)、橘(Citrus reticulata)、橙(Citrus sinensis)、金橘(Citrus japonica)、荔枝(Litchi chinensis)、龍眼(Dimocarpus longan)和無患子(Sapindus saponaria)所萃取而得。 Preferably, the fruit tree extracts are made of apple (Malus pumila), pear (Pyrus communis), lemon (Citrus limon), pomelo (Citrus maxima), orange (Citrus reticulata), orange (Citrus sinensis), golden Extracted from Citrus japonica, Litchi chinensis, Dimocarpus longan and Sapindus saponaria.
較佳者，該等棕櫚類萃取液係由椰子(Cocos nucifera)和棕櫚(Trachycarpus fortunei)所萃取而得。 Preferably, the palm extracts are extracted from coconut (Cocos nucifera) and palm (Trachycarpus fortunei).
較佳者，溶劑係為純水或超臨界CO2。 Preferably, the solvent is pure water or supercritical CO 2 .
較佳者，菌類係取全株以萃取形成該等菌類萃取液，菜葉類植物係取葉子以萃取形成該等菜葉類萃取液，果樹類植物係取樹枝以萃取形成該等果樹類萃取液，而棕櫚類植物係取果實以萃取形成該等棕櫚類萃取液。 Preferably, the whole plant of fungi is extracted to form the fungi extract, the leaves of vegetable plants are extracted to form the vegetable extract, and the branches of fruit trees are extracted to form the fruit tree extract. liquid, and palms are extracted from the fruit to form the palm extracts.
較佳者，菌類、菜葉類植物、棕櫚類植物係在萃取溫度為35℃~50℃)、萃取壓力為25-30MPa的條件下萃取2~4小時，以形成該等菌類萃取液、該等菜葉類萃取液和該等棕櫚類萃取液。 Preferably, fungi, leafy plants and palm plants are extracted for 2 to 4 hours under the conditions that the extraction temperature is 35° C. to 50° C.) and the extraction pressure is 25-30 MPa, so as to form the fungi extracts and the extracts. such as vegetable leaf extracts and such palm extracts.
較佳者，果樹類植物係在萃取溫度為50℃~65℃、萃取壓力為30-35MPa的條件下萃取4~6小時，以形成該等果樹類萃取液。 Preferably, the fruit trees are extracted under the conditions of an extraction temperature of 50° C. to 65° C. and an extraction pressure of 30 to 35 MPa for 4 to 6 hours to form the fruit tree extracts.
如此一來，透過上述之萃取液所調合而成的消毒劑，由於萃取液皆為可食用之菌類及植物製成且製備過程中並無摻入化學藥品，因此所製成之消毒劑就具有天然無毒環保且對人類及環境無害之功效，亦因為這樣，本創作之消毒劑亦可以大面積地噴灑而不需要擔心會產生任何後遺症。 In this way, the disinfectant prepared through the above-mentioned extracts, since the extracts are all made of edible fungi and plants, and no chemicals are mixed in the preparation process, the disinfectant thus produced has It is natural, non-toxic, environmentally friendly and harmless to humans and the environment. Because of this, the disinfectant of this creation can also be sprayed on a large area without worrying about any sequelae.
第1圖，為本發明於滅菌實驗分析之長條圖。 Figure 1 is a bar graph of the present invention in the sterilization experiment analysis.
為使本領域具有通常知識者能清楚了解本發明之內容，謹以下列說明搭配圖式，敬請參閱。 In order to enable those skilled in the art to clearly understand the content of the present invention, please refer to the following descriptions and drawings.
本發明所提供之一種利用可食用之菌類和植物萃取液所調配而成之天然消毒劑，其係藉由複數菌類萃取液、複數菜葉類萃取液、複數果樹類萃取液和複數棕櫚類萃取液所調合製備而成，且混合比例係為該等菌類萃取液1%~3%，該等菜葉類萃取液2%~4%，該等果樹類萃取液5%~8%，該等棕櫚類萃取液8%~12%。其餘則為溶劑。 A natural disinfectant prepared by using edible fungi and plant extracts provided by the present invention is prepared by using a plurality of fungi extracts, a plurality of vegetable leaf extracts, a plurality of fruit tree extracts and a plurality of palm extracts. It is prepared by mixing and preparing the liquid, and the mixing ratio is 1%~3% of these fungi extracts, 2%~4% of these vegetable leaf extracts, 5%~8% of these fruit tree extracts, and the like. Palm extract 8%~12%. The rest are solvents.
其中，該等菌類萃取液係由雙孢蘑菇(Agaricus bisporus)、四孢蘑菇(Agaricus campestris)、兄弟牛肝菌(Boletus fraternus)、褐絨柄牛肝菌(Boletus subvelutipes)、鉛色圓孢牛肝菌(Gyrodon lividus)、褐疣柄牛肝菌(Leccinum scabrum)、厚環乳牛肝菌(Suillus grevillei)、美味羊肚菌(Morchella esculenta)、黑紅菇(Russula nigricans)、簇生黃韌傘(Hypholoma sublateritium)和西口孢離褶傘(Lyophyllum sykosporum)所萃取而得。 Among them, these fungi extracts are made of Agaricus bisporus, Agaricus campestris, Boletus fraternus, Boletus subvelutipes, Plumbago Gyrodon lividus, Leccinum scabrum, Suillus grevillei, Morchella esculenta, Russula nigricans, Umbelliferae Hypholoma sublateritium) and Lyophyllum sykosporum (Lyophyllum sykosporum).
該等菜葉類萃取液係由菠菜(Spinacia oleracea)、萵苣(Lactuca sativa)、荷蘭芹(Petroselinum crispum)、羅勒(Ocimum basilicum)、牛蒡(Arctium lappa)、薄荷(Mentha spicata)、紫蘇(Perilla frutescens var.crispa)和大葉冬青(Ilex latifolia)所萃取而得。 These vegetable leaf extracts are composed of Spinach (Spinacia oleracea), Lettuce (Lactuca sativa), Parsley (Petroselinum crispum), Basil (Ocimum basilicum), Burdock (Arctium lappa), Mint (Mentha spicata), Perilla frutescens var.crispa) and Ilex latifolia.
該等果樹類萃取液係由蘋果(Malus pumila)、西洋梨(Pyrus communis)、檸檬(Citrus limon)、柚(Citrus maxima)、橘(Citrus reticulata)、橙(Citrus sinensis)、金橘(Citrus japonica)、荔枝(Litchi chinensis)、龍眼(Dimocarpus longan)和無患子(Sapindus saponaria)所萃取而得。 These fruit tree extracts are made of apple (Malus pumila), pear (Pyrus communis), lemon (Citrus limon), pomelo (Citrus maxima), orange (Citrus reticulata), orange (Citrus sinensis), kumquat (Citrus japonica) ), Litchi (Litchi chinensis), Longan (Dimocarpus longan) and Sapindus saponaria (Sapindus saponaria).
該等棕櫚類萃取液係由椰子(Cocos nucifera)和棕櫚(Trachycarpus fortunei)所萃取而得。 These palm extracts are derived from coconut (Cocos nucifera) and palm (Trachycarpus fortunei).
上述四類植物萃取液，所取材的部分各不相同，菌類是取全株，菜葉類取葉子，果樹類取樹枝，棕櫚類取果實。並且，上述四類萃取液在製作前，都必須先經篩選(去除霉爛或蟲蛀的原料)，然後洗淨、乾燥。乾燥的方法基本有二： The above-mentioned four types of plant extracts have different parts, the whole plant is taken for fungi, the leaves are taken for vegetable leaves, the branches are taken from fruit trees, and the fruits are taken from palms. In addition, the above four types of extracts must be screened (removal of moldy or moth-eaten raw materials) before being produced, and then washed and dried. There are basically two drying methods:
1.冷凍乾燥：絕對壓力在20~100Pa(真空度)，溫度為零下30℃~零下40℃，乾燥時間為8~12小時；菌類、菜葉類、棕櫚類原料多用此法。 1. Freeze drying: the absolute pressure is 20~100Pa (vacuum degree), the temperature is minus 30℃~minus 40℃, and the drying time is 8~12 hours; this method is mostly used for fungi, vegetable leaves and palm materials.
2.烘箱乾燥：溫度為50℃~60℃，乾燥時間為8~12小時；果樹類原料多用此法。 2. Oven drying: the temperature is 50℃~60℃, and the drying time is 8~12 hours; this method is mostly used for fruit tree raw materials.
在本發明中，係使用純水或超臨界CO2為溶劑，並以低溫(35℃~65℃)高壓(25-35MPa)方式萃取，溫度不盡相同，菌類、菜葉類、棕櫚類原料的萃取溫度(35℃~50℃)、萃取壓力(25-30MPa)較低，萃取時間2~4小時；果樹類原料的萃取溫度(50℃~65℃)、萃取壓力(30-35MPa)較高，萃取時間4~6小時。 In the present invention, pure water or supercritical CO 2 is used as a solvent, and the extraction is performed at a low temperature (35 ° C ~ 65 ° C) and a high pressure (25-35 MPa), and the temperature is different. The extraction temperature (35°C~50°C) and extraction pressure (25-30MPa) are lower, and the extraction time is 2~4 hours; the extraction temperature (50°C~65°C) and extraction pressure (30-35MPa) of fruit tree raw materials are relatively High, the extraction time is 4 to 6 hours.
透過上述的製備前的準備工序、後續的萃取工序以及最後依照限定的調合比例進行混合後就能夠得到本發明所提供之天然消毒劑。以下係藉由各家不同的檢驗場所所出具之檢測結果，以進一步展示本發明所提供之天然消毒劑確實具有良好之消毒效果。 The natural disinfectant provided by the present invention can be obtained through the above-mentioned preparation process before the preparation, the subsequent extraction process and finally mixing according to the limited blending ratio. The following are the test results issued by different inspection sites to further demonstrate that the natural disinfectant provided by the present invention indeed has a good disinfection effect.
請參閱下報告1A-1E，其係為本發明於農藥定量分析檢測之結果報告示意圖。透過此SGS報告就可以清楚知悉本發明在依據衛生福利部於103.07.03公告之食品中殘留農藥檢驗方法-多重殘留分析方法(五)(部授食字第1031900615號公告)，以液相層析串聯質譜儀(LC/MS/MS)及以氣相層析串聯式質譜儀(GC/MS/MS)檢測後，常見的農藥如亞滅培、丁基滅必蝨、免敵克等310種 農藥均未檢出，因此可以確定本發明之天然消毒劑並不是依靠添加農藥之類的化學藥品來達到消毒的效果。 Please refer to the following reports 1A-1E, which are schematic diagrams of the results report of the present invention in the quantitative analysis and detection of pesticides. Through this SGS report, we can clearly know that the present invention is based on the inspection method of pesticide residues in food announced by the Ministry of Health and Welfare on 103.07.03-Multiple Residue Analysis Method (V) (Bu Fu Shi Zi No. 1031900615 Announcement). After detection by gas chromatography tandem mass spectrometer (LC/MS/MS) and gas chromatography tandem mass spectrometer (GC/MS/MS), common pesticides such as imiperipa, butylate, and 310 kind No pesticides were detected, so it can be confirmed that the natural disinfectant of the present invention does not rely on the addition of chemicals such as pesticides to achieve the effect of disinfection.
請參閱下報告2，其係為本發明於金黃色葡萄球菌之抗菌測試結果報告圖。透過此SGS報告就可以清楚知悉在加入本發明所提供之天然消毒劑後，原本有3.95×105CFU/mL接菌量的金黃色葡萄球菌於24小時後，就變為僅只有1.30×101CFU/mL，亦即抑菌率係大於99.9%，進而確認本發明確實有良好之消毒效果。
Please refer to the following
請參閱下報告3，其係為本發明於大腸桿菌之抗菌測試結果報告圖。透過此SGS報告就可以清楚知悉在加入本發明所提供之天然消毒劑後，原本有3.00×105CFU/mL接菌量的大腸桿菌於24小時後，就變為小於1CFU/mL，亦即抑菌率係大於99.9%，進而確認本發明確實有良好之消毒效果。
Please refer to the
請參閱下報告4，其係為本發明於白色念珠菌之抗菌測試結果報告圖。透過此SGS報告就可以清楚知悉在加入本發明所提供之天然消毒劑後，原本有2.90×105CFU/mL接菌量的白色念珠菌於24小時後，就變為小於1CFU/mL，亦即抑菌率係大於99.9%，進而確認本發明確實有良好之消毒效果。 Please refer to the following report 4, which is a graph of the antibacterial test result report on Candida albicans of the present invention. Through this SGS report, it can be clearly known that after adding the natural disinfectant provided by the present invention, the Candida albicans with an inoculation amount of 2.90×10 5 CFU/mL became less than 1 CFU/mL after 24 hours, and also That is, the bacteriostatic rate is greater than 99.9%, and it is further confirmed that the present invention has a good disinfection effect.
此外，本發明所提供之天然消毒劑係有在室內進行噴灑實驗測試其在室內的消毒效能，其實驗係搭配能產生次微米級氣霧的EP828大型移動式超音波震盪氣霧機，將天然消毒劑以分子方式快速地釋放於專案會議場的各區會議室與辦公室空間之中，每立方公尺釋放1毫升(整體空間體積約45700立方公尺)，噴霧30分鐘，再以德國默克(Merck)的MAS-100空氣菌落收集機(採樣流量為100L/min)進行空氣菌落採樣分析，結果顯示噴霧後空氣中懸浮菌落均明顯降低，一開始於空間中採集菌落數(CFU/m3)係為480，在超音波氣霧使用產生10分鐘變為130(72.9%)，產生30分鐘後則變為20(95.8%)，而各區室內平均滅菌率為92.5%，空氣菌落減少效能亦為良好將1：500稀釋後的空氣清淨劑以超音波震盪方式，上述數據證實釋放1.0mL/m3的氣霧在各類室內空間30分鐘後，均能有效消除空氣懸浮菌落，平均滅菌率為92.5%；唯室內空間的密閉性、菌落數、人員數、停留時間與空調系統均能影響菌落降低效能，本研究經由實場操作，獲得30分鐘後能達九成降低率，依然可見本發明在室內消毒上的卓越成效。 In addition, the natural disinfectant provided by the present invention is sprayed indoors to test its disinfection efficiency indoors. The experiment is matched with EP828 large-scale mobile ultrasonic vibration aerosol machine that can generate sub-micron aerosols. The disinfectant is rapidly released in the conference room and office space of each area of the project conference venue in a molecular manner, releasing 1 ml per cubic meter (the overall space volume is about 45,700 cubic meters), sprayed for 30 minutes, and then sprayed with German Merck. (Merck) MAS-100 air colony collector (sampling flow rate of 100L/min) was used for air colony sampling analysis. The results showed that the suspended colonies in the air were significantly reduced after spraying, and the number of colonies (CFU/m3) was collected in the space at the beginning. The system is 480, and it becomes 130 (72.9%) after 10 minutes of ultrasonic aerosol use, and 20 (95.8%) after 30 minutes of generation. The average sterilization rate in each zone is 92.5%, and the air colony reduction effect is also In order to make the air purifier diluted 1:500 by ultrasonic vibration, the above data confirms that the aerosol of 1.0mL/m3 can effectively eliminate air suspension colonies after 30 minutes in various indoor spaces, and the average sterilization rate is 92.5%; only the airtightness of the indoor space, the number of colonies, the number of personnel, the residence time and the air-conditioning system can affect the colony reduction efficiency. In this study, through the field operation, the reduction rate can reach 90% after 30 minutes, and the present invention can still be seen. Excellent results in indoor disinfection.
請參閱第1圖，其係為本發明滅菌實驗分析之長條圖。此長條圖係藉由下方之表1內的數據而製作而成。 Please refer to Figure 1, which is a bar graph of the sterilization experiment analysis of the present invention. This bar graph was created from the data in Table 1 below.
此表格係為本發明所提供之天然消毒劑在稀釋200倍後，進行參照環檢所-環境衛生用消毒劑藥效試驗測定法所得出之數據。藉由此數據以及圖表就可以清楚知悉本發明在稀釋200倍後依然具有良好的消毒效果。 This table is the data obtained by referring to the Environmental Inspection Institute-Environmental Sanitation Disinfectant Efficacy Test Method after the natural disinfectant provided by the present invention is diluted 200 times. From the data and the chart, it can be clearly known that the present invention still has a good disinfection effect after being diluted 200 times.
請參閱下報告5，其係為本發明於多種致病菌之抗菌測試結果報告圖。透過此台美出示之檢驗報告就可以清楚知悉在加入本發明所提供之天然消毒劑並檢驗後，沙門氏菌、金黃色葡萄球菌、大腸桿菌和產芽孢梭菌皆為陰性反應，進而確認本發明確實有良好之消毒效果。 Please refer to the following report 5, which is a graph of the report of the antibacterial test results of the present invention against various pathogenic bacteria. Through the inspection report presented by Taimei, we can clearly know that after adding the natural disinfectant provided by the present invention and testing, Salmonella, Staphylococcus aureus, Escherichia coli and Clostridium sporogenes are all negative reactions, thereby confirming that the present invention is true Has a good disinfection effect.
請參閱下報告6，其係為本發明於本發明於流感病毒H1N1之抗菌測試結果報告圖。透過此亞威生物科技出示之檢驗報告就可以清楚知悉在加入本發明所提供之天然消毒劑後，3個甲型流感病毒H1N1試驗組的病毒數值(IgTCID50/mL)於作用10分鐘後係皆小於0.5，亦即抑菌率係大於99.99%，進而確認本發明確實有良好之消毒效果。 Please refer to the following report 6, which is a graph of the present invention's antibacterial test result report on influenza virus H1N1. Through the inspection report provided by Yawei Biotechnology, we can clearly know that after adding the natural disinfectant provided by the present invention, the virus value (IgTCID 50 /mL) of the three influenza A virus H1N1 test groups is the same after 10 minutes of action. All are less than 0.5, that is, the bacteriostatic rate is greater than 99.99%, thus confirming that the present invention has a good disinfection effect.
.參閱下報告7，其係為本發明於沙雷氏菌之抗菌測試結果報告圖。透過此亞威生物科技出示之檢驗報告就可以清楚知悉在加入本發明所提供之天然消毒劑後，在加入前有2.7×106CFU/mL菌種濃度的沙雷氏菌於24小時後進行檢測，發現消毒率係大於99.76%，進而確認本發明確實有良好之消毒效果。 . Refer to the following report 7, which is a graph of the report of the antibacterial test results of the present invention against Serratia. Through the inspection report produced by Yawei Biotechnology, it can be clearly known that after adding the natural disinfectant provided by the present invention, the Serratia bacteria with a concentration of 2.7×10 6 CFU/mL strains were added 24 hours later. Detection, found that the disinfection rate is greater than 99.76%, and then confirm that the present invention does have a good disinfection effect.
請參閱下報告8，其係為本發明於副溶血性弧菌之抗菌測試結果報告圖。透過此亞威生物科技出示之檢驗報告就可以清楚知悉在加入本發明所提供之天然消毒劑後，在加入前有3.8×106CFU/mL菌種濃度的副溶血性弧菌於24小時後進行檢測，發現消毒率係大於99.99%，進而確認本發明確實有良好之消毒效果。 Please refer to the following report 8, which is a graph of the antibacterial test result report on Vibrio parahaemolyticus of the present invention. Through the inspection report produced by Yawei Biotechnology, we can clearly know that after adding the natural disinfectant provided by the present invention, there is Vibrio parahaemolyticus with a strain concentration of 3.8×10 6 CFU/mL before adding 24 hours later. Detected and found that the disinfection rate was greater than 99.99%, thus confirming that the present invention indeed has a good disinfection effect.
請參閱下報告9，其係為本發明於單核李斯特菌之抗菌測試結果報告圖。透過此亞威生物科技出示之檢驗報告就可以清楚知悉在加入本發明所提供之天然消毒劑後，在加入前有6.2×105CFU/mL菌種濃度的單核李斯特菌於24小時後進行檢測，發現消毒率係大於99.92%，進而確認本發明確實有良好之消毒效果。 Please refer to the following report 9, which is a graph of the antibacterial test result report on Listeria monocytogenes of the present invention. Through the inspection report produced by Yawei Biotechnology, it can be clearly known that after adding the natural disinfectant provided by the present invention, there is a concentration of 6.2×10 5 CFU/mL of Listeria monocytogenes before adding 24 hours later. Detected and found that the disinfection rate was greater than 99.92%, thus confirming that the present invention indeed has a good disinfection effect.
請參閱下報告10，其係為本發明於銅綠假單胞菌之抗菌測試結果報告圖。透過此亞威生物科技出示之檢驗報告就可以清楚知悉在加入本發明所提供之天然消毒劑後，在加入前有2.4×106CFU/mL菌種濃度的銅綠假單胞菌於24小時後進行檢測，發現消毒率係大於99.98%，進而確認本發明確實有良好之消毒效果。 Please refer to the following report 10, which is a report diagram of the antibacterial test results of the present invention against Pseudomonas aeruginosa. Through the inspection report produced by Yawei Biotechnology, we can clearly know that after adding the natural disinfectant provided by the present invention, there is Pseudomonas aeruginosa with a strain concentration of 2.4×10 6 CFU/mL before adding 24 hours later. Detected and found that the disinfection rate was greater than 99.98%, thus confirming that the present invention indeed has a good disinfection effect.
請參閱下報告11，其係為本發明於大腸桿菌之抗菌測試結果報告圖。透過此亞威生物科技出示之檢驗報告就可以清楚知悉在加入本發明所提供之天然消毒劑後，在加入前有2.3×106CFU/mL菌種濃度的大腸桿菌於24小時後進行檢測，發現消毒率係大於99.98%，進而確認本發明確實有良好之消毒效果。 Please refer to the following report 11, which is a report chart of the antibacterial test results of the present invention in Escherichia coli. Through the inspection report presented by Yawei Biotechnology, we can clearly know that after adding the natural disinfectant provided by the present invention, Escherichia coli with a concentration of 2.3×10 6 CFU/mL strains was detected 24 hours later. It is found that the disinfection rate is greater than 99.98%, and it is confirmed that the present invention has a good disinfection effect.
請參閱下報告12，其係為本發明於白色念珠菌之抗菌測試結果報告圖。透過此亞威生物科技出示之檢驗報告就可以清楚知悉在加入本發明所提供之天然消毒劑後，在加入前有2.4×106CFU/mL菌種濃度的白色念珠菌於24小時後進行檢測，發現消毒率係大於99.98%，進而確認本發明確實有良好之消毒效果。 Please refer to the following report 12, which is a graph of the antibacterial test result report on Candida albicans of the present invention. Through the inspection report presented by Yawei Biotechnology, it can be clearly known that after adding the natural disinfectant provided by the present invention, the Candida albicans with a concentration of 2.4×10 6 CFU/mL strains was detected 24 hours later. , found that the disinfection rate was greater than 99.98%, and then confirmed that the present invention indeed has a good disinfection effect.
請參閱下報告13，其係為本發明於沙門氏菌之抗菌測試結果報告圖。透過此亞威生物科技出示之檢驗報告就可以清楚知悉在加入本發明所提供之天然消毒劑後，在加入前有7.8×105CFU/mL菌種濃度的沙門氏菌於24小時後進行檢測，發現消毒率係大於99.93%，進而確認本發明確實有良好之消毒效果。 Please refer to the following report 13, which is the report chart of the antibacterial test results of the present invention against Salmonella. Through the inspection report presented by Yawei Biotechnology, we can clearly know that after adding the natural disinfectant provided by the present invention, there is a concentration of 7.8×10 5 CFU/mL of Salmonella 24 hours later. The disinfection rate is greater than 99.93%, thus confirming that the present invention has a good disinfection effect.
綜上所述，本創作所提供之天然消毒劑目前確實已通過SGS「不含310項農藥檢測」。此外還通過SGS、台美檢驗科技、亞威生物科技「H1N1病毒滅活率>99.9%」且「金黃色葡萄球菌、大腸桿菌、白色念珠菌......等9種之抑菌/消毒率檢測」(皆在99.76%~99.99%)。因為以證實確實有效且又是天然且對人體無害，該天然消毒劑就可擴大運用在醫療院所場域之消毒，預計可達成90%以上的有效滅菌率，並符合環保署《室內空氣品質標準》。此種創新的綠色產品與工法，預期對醫療院所室內消毒將產生革命性的影響。 To sum up, the natural disinfectant provided by this creation has indeed passed the SGS "310 pesticide-free test". In addition, it has passed the antibacterial / Disinfection rate detection" (all in the range of 99.76%~99.99%). Because it is proved to be effective, natural and harmless to the human body, the natural disinfectant can be widely used in the disinfection of medical institutions. standard". Such innovative green products and construction methods are expected to have a revolutionary impact on indoor disinfection of medical institutions.
惟，以上所述者，僅為本發明之較佳實施例而已，並非用以限定本發明實施之範圍；故在不脫離本發明之範圍下所作之均等變化與修飾，皆應涵蓋於本發明之專利範圍內。 However, the above descriptions are only preferred embodiments of the present invention and are not intended to limit the scope of implementation of the present invention; therefore, equivalent changes and modifications made without departing from the scope of the present invention should be included in the present invention. within the scope of the patent.
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| CN108142451A (en) * | 2017-12-13 | 2018-06-12 | 中央民族大学 | A kind of extractive of perilla and its application as Genes For Plant Tolerance fungus |
| CN108653342A (en) * | 2018-07-09 | 2018-10-16 | 海南医学院 | A kind of microbial antibacterial extract and its preparation method and application |
| CN111066828A (en) * | 2019-11-21 | 2020-04-28 | 吴杨 | Plant extraction sterilization and disinfection composition special for farm and preparation method thereof |
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| CN103843838A (en) * | 2012-12-05 | 2014-06-11 | 于海军 | Plant source bactericidal synergist, extraction process and application thereof to agricultural bactericide |
| CN108142451A (en) * | 2017-12-13 | 2018-06-12 | 中央民族大学 | A kind of extractive of perilla and its application as Genes For Plant Tolerance fungus |
| CN108653342A (en) * | 2018-07-09 | 2018-10-16 | 海南医学院 | A kind of microbial antibacterial extract and its preparation method and application |
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