TWI741843B - The protein measurement apparatus and operation method thereof - Google Patents

Abstract

The invention is to provide a protein measurement apparatus and operation method thereof, including power supply, electromagnetic-wave generator, electromagnetic-wave detection circuit, micro-controller and display, and method thereof.

Description

Body fluid protein concentration detection device and operation method thereof

The present invention provides a body fluid protein concentration detection device and its operating method, in particular to a micro-electromagnetic wave generator for body fluid protein concentration detection and its operating method.

Protein is the main component of human cell tissue, and when the protein concentration is abnormal, it may cause human diseases. For example, diabetes in the human body is a chronic metabolic disorder of the human body, and there are two main causes of diabetes. On the one hand, the pancreas cannot produce enough insulin protein hormone, and on the other hand, it is also the cells' ability to produce insulin protein hormone. The role of resistance produces resistance.

The medical community believes that due to the interaction of genetic, environmental, chemical toxins, and microorganisms, the pancreas in the body produces insufficient insulin secretion or resistance to the action of insulin in the body, which reduces the ability to utilize sugars or even completely fails. Utilization can cause high blood sugar, sugar in urine, and incorrect metabolism of protein and fat in the patient’s body. often.

Diabetes is a chronic disease that cannot be cured. Once suffering from diabetes, patients must actively pay attention to blood sugar control throughout their lives, avoid diabetes threatening their lives, and avoid serious complications caused by diabetes, such as cardiovascular disease and retinopathy. , Neuropathy, etc. affect the quality of life of patients.

The number of people suffering from diabetes in the world was 124 million in 1997, and in 2014, it is estimated that 422 million adults worldwide have diabetes, and due to the rapid increase in the number of people with diabetes Its complications have even caused a financial burden to the society, and the quality of life of patients has dropped rapidly.

With the aging of the population, changes in lifestyles and dietary patterns, diabetes has become one of the top four causes of death among Chinese in recent years. It is estimated that the population of diabetes patients in Taiwan has reached one million, and more than 90% of them are non-insulin-dependent, also known as type 2 diabetes, and because most of the patients are over 40 years old, and most often at 55 Diabetes develops after age, so it is also called adult-type diabetes.

It is estimated that nearly half of people with diabetes do not realize that they are sick. The reason is that the symptoms of diabetes often occur slowly and are often difficult to detect at the beginning. Diabetes is the killer of the systemic vascular system. The chronic comorbidities it brings include small vessel disease, such as retinopathy, kidney disease, neuropathy; and macrovascular disease, also known as arteriosclerosis; because once the disease is not well controlled, it is easy to cause Heart disease and stroke can also cause complications such as eyes, kidneys, blood vessels in the lower limbs, and even blindness, kidney dialysis and amputation.

Therefore, from the perspective of preventive medicine, if there is a simple detection method to diagnose insulin resistance and early diabetes early, it will be able to avoid type 2 diabetes, which can reduce national medical expenditures and benefit the general public. Due to insulin resistance and early diabetes patients, the blood or saliva insulin concentration will increase. If the changes in insulin concentration in body fluids can be actively monitored early, the early diagnosis of hyperinsulinemia (ie insulin resistance or pre-diabetes) can be made. Pre-diabetes can be improved through dietary adjustments and exercise to prevent them from developing diabetes. Once diabetes develops, the death of pancreatic β-cells cannot cure the disease, and patients must take medication for life to monitor blood sugar. Since insulin is a protein hormone secreted by pancreatic β cells into the blood, it has the function of lowering blood sugar. Therefore, taking the detection of insulin concentration in body fluids as an example, a novel protein detection device was invented. In addition to detecting changes in insulin concentration in body fluids, it can also be extended to detect changes in the concentration of other disease-related proteins in body fluids.

A body fluid protein concentration detection device of the present invention includes a host device, a display, and a test strip device, wherein the host device is connected to the display, and the host device is connected to the test strip device.

A body fluid protein concentration detection device of the present invention, wherein the host device includes a power supply, a miniature electromagnetic wave generator, an electromagnetic wave reflection signal detector, a microcontroller, a display, and the power supply is connected Connected to the miniature electromagnetic wave generator, the miniature electromagnetic wave generator is then connected to the electromagnetic wave reflection signal detector, and the electromagnetic wave reflection signal detector is connected to the microcontroller.

The operating method of a body fluid protein concentration detection device of the present invention first provides a saliva sample in a test strip device, activates a micro electromagnetic wave generator, and uses the micro electromagnetic wave generator to emit electromagnetic waves to the tester's saliva sample in the test strip device Scan signal. Then, collect the electromagnetic reflection signal after the electromagnetic wave scanning signal emitted by the micro electromagnetic wave generator to the tester's saliva sample. Continuing, the electromagnetic wave reflection signal detector is used to detect the electromagnetic wave reflection signal of the tester's saliva sample, so as to detect the electromagnetic wave reflection signal.

Then, according to the electromagnetic reflection signal of the electromagnetic reflection signal of the saliva sample of the tester, and the characteristic curve is compared and calculated, the concentration of insulin protein in the tester's saliva is determined.

Finally, the monitor displays the tester's insulin protein test result, which can display the concentration of insulin protein contained in the tester's saliva sample, so as to assist in determining whether he has hyperinsulinemia (ie insulin resistance or pre-diabetes).

Due to its small size, the present invention can be installed in the field of 3C household appliances or communication equipment, such as smart phones.

The invention can detect insulin protein at any time from the perspective of preventive medicine, that is, it can monitor whether hyperinsulinemia (that is, insulin resistance or pre-diabetes) occurs at any time.

In addition to assisting normal people to avoid diabetes, the present invention can also allow normal people to actively pay attention to the control of their own insulin concentration, and warn them to maintain and ensure good normal living habits.

The present invention monitors the concentration of insulin protein in body fluids in a non-invasive manner. When the insulin protein shows an increasing trend and shows that it may be a sign of diabetic patients, the patient can remind and take necessary actions to prevent diabetes.

The invention can also assist normal people to check their daily habits to ensure a good life style and avoid becoming diabetic patients.

For diabetic patients, the present invention can assist in understanding the concentration of insulin in the blood, thereby reminding medical staff to put in appropriate insulin to control the blood glucose concentration of diabetic patients.

Compared with the traditional enzyme-linked immunosorbent assay (ELISA) detection method, the present invention has higher sensitivity for detecting insulin, and the present invention has high reproducibility for multi-person detection, as well as single-person detection. The high reproducibility.

More particularly, the device of the present invention is simple in structure, has a faster detection speed, and has the advantages of accurate detection results.

100: host device

101: power supply

102: Miniature electromagnetic wave generator

103: Electromagnetic wave reflection signal detector

104: Microcontroller

105: display

106: Test piece device

For the aforementioned and many other advantages of the present invention, please refer to the following drawings A detailed description of a preferred embodiment will be shown more clearly, which is as follows:

Figure 1 shows a body fluid protein concentration detection device of the present invention.

Figure 2 shows the method of operation of a body fluid protein concentration detection device of the present invention.

Figure 3 shows the characteristic curve of the insulin protein concentration after the detection and operation of the body fluid protein concentration detection device of the present invention.

Figure 4 shows the precise detection value of the insulin protein concentration after the detection and operation of the body fluid protein concentration detection device of the present invention.

Hereinafter, please refer to the description and description of the accompanying drawings to describe the embodiments of the present invention. In the drawings, the same component symbols represent the same components, and for clarity, the size or thickness of the components may be exaggerated.

As shown in Figure 1, a body fluid protein concentration detection device of the present invention includes a host device 100, a display 105, and a test strip device 106, wherein the host device 100 is connected to the display 105, and the host device 100 is connected to the test strip device 106.

As shown in Figure 1, a body fluid protein concentration detection device of the present invention, wherein the host device 100 includes a power supply (Power supply) 101, a miniature electromagnetic-wave generator (Electromagnetic-wave generator) 102, an electromagnetic wave reflection signal detector ( Electromagnetic-wave detection circuit) 103, and a microcontroller (Microcontroller) 104. The power supply 101 is connected to the micro electromagnetic wave generator 102, the micro electromagnetic wave generator 102 is then connected to the electromagnetic wave reflection signal detector 103, and the electromagnetic wave reflection signal detector 103 is connected to the microcontroller 104.

It is particularly reminded here that the present invention is a non-invasive body fluid protein concentration detection device that uses insulin as a means to detect protein concentration. Therefore, in this description, it is obvious that the detection of insulin is used as a test example. As an explanation of the implementation steps. The above-mentioned selection design is mainly because insulin is also a protein hormone, which is secreted by pancreatic β cells in the pancreas. Insulin can participate in the regulation of carbohydrate and fat metabolism, control blood sugar balance, and promote the liver and skeletal muscle cells to convert blood glucose into sugar for use, that is, blood sugar into glycogen and intracellular glucose. Therefore, when the human body lacks effective insulin, it will lead to high blood sugar and even diabetes.

In addition, because saliva is a complex mixture that not only contains various proteins, but also DNA, RNA, fatty acids, and various microorganisms, and because various protein components in blood are also present in saliva, saliva can reflect various proteins in blood. At present, it is known that there are about 1166 kinds of proteins in human saliva, and most of the proteins can be found in plasma and tear fluid at the same time.

As shown in Fig. 1 again, in a body fluid protein concentration detection device of the present invention, the power supply 101 has the function of providing power.

As shown in Figure 1, a body fluid protein concentration detection device of the present invention, in which the micro electromagnetic wave generator 102 has the function of providing scanning reception and sensing, that is, when the micro electromagnetic wave generator 102 has an electromagnetic scanning signal that emits electromagnetic waves , And the function of receiving electromagnetic reflection signals of electromagnetic waves, it can achieve the functions of scanning receiving and sensing. Since the miniature electromagnetic wave generator 102 is so small that it can even be mounted on an integrated circuit chip, it can also be adapted to be used in the field of 3C home appliances and communications, such as smart phones. In particular, the miniature electromagnetic wave generator 102 can emit ^{a waveband of about 60×10 9} Hz to detect the reflected wave of insulin protein.

As shown in Figure 1, the body fluid protein concentration detection device of the present invention, in which the reflected signal is an electromagnetic wave, and its intensity and phase will be determined according to the dielectric constant of the material. Because the concentration of different insulin proteins in the tester's saliva carried by the test strip device 106 can change the dielectric constant of the saliva, the concentration of the tester's saliva can be determined by the characteristic curve. The characteristic curve is a sample of the standard concentration of insulin to obtain the characteristic curve.

As shown in FIG. 1, in a body fluid protein concentration detection device of the present invention, the electromagnetic wave reflection signal detector 103 has the function of detecting the electromagnetic wave reflection signal generated by the micro electromagnetic wave generator 102.

As shown in Figure 1, a body fluid protein concentration test of the present invention The measurement device, in which the microcontroller 104 has the function of calculating and analyzing the reflected wave electromagnetic wave reflected signal of the electromagnetic wave, so as to achieve the function of comparing and analyzing the detection result.

As shown in Fig. 1, in a body fluid protein concentration detection device of the present invention, the display 105 has the function of displaying the analysis and detection results.

As shown in Figure 1, a body fluid protein concentration detection device of the present invention, wherein the test strip device 106 can provide a load to handle the saliva of the tester, as a preparation for the test strip, including adding a specific protein detection reagent to the test strip device 106 , Wherein the specific protein detection reagent includes an insulin protein detection reagent.

As shown in Figure 2, the operating method of a body fluid protein concentration detection device of the present invention. As shown in step 201 in Figure 2, the micro electromagnetic wave generator 102 is first activated, and the micro electromagnetic wave generator 102 is used to process the test strip device 106 Of test subjects’ saliva emits scanning signals of electromagnetic waves.

As shown in FIG. 2, the operating method of the body fluid protein concentration detection device of the present invention is shown in step 202 in FIG.

The operating method of the body fluid protein concentration detection device of the present invention is still shown in Figure 2. As shown in step 203 in Figure 2, continue, the electromagnetic wave reflection signal detector 103 is used to detect the electromagnetic wave reflection signal of the tester’s saliva. In order to detect the electromagnetic wave reflection signal.

As shown in Figure 2, a body fluid protein concentration of the present invention The operation method of the degree detection device is as shown in step 204 in Fig. 2. Then, the micro-controller 104 confirms that the electromagnetic wave reflection signal is the insulin protein concentration of the tester’s saliva from the tester’s test data accessed previously. concentration).

Next, as shown in step 205 in Figure 2, the method of operation of a body fluid protein concentration detection device is based on the aforementioned electromagnetic wave reflection signal of the tester’s saliva and compared with the characteristic curve to determine the insulin protein contained in the tester’s saliva. concentration.

Finally, the operation method of a body fluid protein concentration detection device shown in step 206 in Figure 2 uses the display 105 to display the test results of insulin protein in the tester’s saliva, that is, to display the concentration of insulin protein in the tester’s saliva, thereby Assist in judging whether you have diabetes.

Figure 3 shows a characteristic curve of the insulin protein concentration of a body fluid protein concentration detection device of the present invention. The horizontal axis is the insulin protein concentration, and the vertical axis is the electromagnetic wave reflection signal.

Figure 4 shows that the present invention is an accurate detection value of the insulin protein concentration after the operation of the body fluid protein concentration detection device. From left to right, the "test piece number" and the actual "insulin protein concentration" are displayed. The detected "insulin protein concentration" and the "error rate" on the rightmost side show that the "error rate" is extremely low, not exceeding the error rate of 1.9%. By comparison, it can be seen that the present invention is useful for detecting insulin protein concentration. Has a very high precision detection value.

In summary, due to the small size of the present invention, it can be installed in the field of 3C home appliances or communication equipment, such as smart phones, so the present invention can carry out diabetes detection at any time, that is, it can monitor whether diabetes occurs at any time. .

The present invention monitors the body's protein in a non-invasive manner. When the insulin protein in the saliva of a normal person shows an increasing trend, which is a sign of hyperinsulinemia (i.e. insulin resistance or pre-diabetes) patients, it can alert and respond by itself. Take necessary actions to prevent diabetes. The present invention can also assist normal people to check their daily habits to ensure their normal living habits without becoming a diabetic patient.

Compared with diabetic patients, the present invention can also assist in understanding the insulin protein content in their saliva, thereby assisting medical staff to provide appropriate insulin. Moreover, the present invention can alert the diabetic patients' personal life habits, ensure their normal life habits, and avoid exacerbating their diabetes.

Therefore, in addition to preventing normal people from suffering from diabetes, the present invention can also remind normal people to actively pay attention to blood sugar control and maintain good living habits. In other words, the present invention can help remind normal people to avoid the occurrence of diabetes, and can prevent diabetes from threatening their lives, so as to avoid affecting the quality of life of normal people.

The above are only the preferred embodiments of the present invention, and are not intended to limit the scope of patent application of the present invention; all other equivalent changes or modifications made without departing from the spirit of the present invention should be included in the following Shen Please be within the scope of the patent.

100: host device

101: power supply

102: Miniature electromagnetic wave generator

103: Electromagnetic wave reflection signal detector

104: Microcontroller

105: display

106: Test piece device

Claims (4)

A device for detecting body fluid protein concentration by using tester's saliva at least includes: a host device, at least including: a power supply, the power supply having the function of providing a power; a miniature electromagnetic wave generator, the miniature electromagnetic wave generator It has the functions of providing a scanning receiving and sensing, wherein the miniature electromagnetic wave generator includes an integrated circuit chip which can be installed; an electromagnetic wave reflection signal detector, the electromagnetic wave reflection signal detector has the function of providing detection of the miniature electromagnetic wave generator An electromagnetic wave reflection signal function; and a microcontroller, the microcontroller having a function of providing a calculation and analysis of a reflected wave electromagnetic wave reflection signal of an electromagnetic wave; wherein the power supply is connected to the miniature electromagnetic wave generator, and the miniature electromagnetic wave is generated The device is connected to the electromagnetic wave reflection signal detector, and the electromagnetic wave reflection signal detector is connected to the microcontroller; a display, wherein the display has a function of displaying analysis and detection results; and a test strip device, which can provide Carry a tester’s saliva as a preparation for a test piece, The host device is connected to the display, and the host device is connected to the test strip device. As for the detection device described in item 1 of the scope of patent application, the miniature electromagnetic wave generator is suitable for a communication field. An operating method of a body fluid protein concentration detection device using the saliva of a tester as in the scope of the patent application, at least comprising: activating a miniature electromagnetic wave generator, and using the miniature electromagnetic wave generator to send a scan on a tester's saliva sample Signal, wherein the micro electromagnetic wave generator includes a reflection signal that can be installed on an integrated circuit chip; collects a reflection signal emitted by the micro electromagnetic wave generator from the scanning signal to the tester’s saliva; detects the signal with an electromagnetic wave reflection signal detector An electromagnetic wave reflection signal of the reflection signal of the tester’s saliva sample; from a detection data of the tester’s saliva sample accessed by a microcontroller, it is confirmed that the detected electromagnetic wave reflection signal is that of the tester’s saliva sample An insulin protein concentration; calculating according to an electromagnetic wave reflection signal of the tester’s saliva and comparing it with a characteristic curve to determine the insulin protein concentration contained in the tester’s saliva sample; and displaying the tester on a display A detection result of the saliva sample, wherein the display has a function of displaying the analysis detection result. The operation method described in item 3 of the scope of patent application, wherein the miniature electromagnetic wave generator is suitable for a communication field.

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