TWI719303B - Use of rosa roxburghii fruit extracts for manufacture of composition for cell rejuvenation - Google Patents

Abstract

The present invention provides use of a Rosa roxburghii fruit extract for manufacture of composition for cell rejuvenation. The Rosa roxburghii fruit extract is prepared by extraction of Rosa roxburghii fruits using water, alcohols, or mixtures of water and alcohols as solvents. This extract can promote DNA repair, reduce cell damage caused by oxidative stress or inflammation, maintain normal functions of genes, proteins, and mitochondria, stimulate the telomerase reaction, and reduce telomere damage due to chemicals and UV exposure, thus facilitating cell rejuvenation.

Description

Use of Prickly Pear extract for preparing cell rejuvenation composition

The present invention relates to the health-care use of a prickly pear extract, in particular to the use of a prickly pear extract to prepare a cell rejuvenation composition.

With the increase of the average age of the population, having a healthy and high-quality elderly life has become one of the focuses of public attention, and scientific research on aging has also become more and more important. There are many reasons for individual aging. At the molecular and cellular levels, possible internal mechanisms include shortening of telomeres at the end of chromosomes, gene mutations, poor protein synthesis efficiency, decreased mitochondrial function, and cell morphological changes. In addition, free radicals (such as reactive oxygen species) generated by physical or chemical factors in the surrounding environment of cells can damage the chromosomal deoxyribonucleic acid (DNA), proteins, and lipid biofilms of cells, and individuals respond to such cell damage or Once the inflammatory response initiated by microbial invasion develops into chronic inflammation, it will aggravate cell damage, thus accelerating the aging of cells and individuals.

Anti-aging methods include obtaining natural antioxidants through diet to enhance the individual's ability to scavenge free radicals, and anti-inflammatory drugs to inhibit chronic inflammation in the body. In view of the above-mentioned complex aging causes, the anti-aging method designed for a single target is obviously not effective. Although the emerging stem cell therapy has great anti-aging potential, it is still in the development stage and the treatment cost is high. Therefore, it is necessary to develop a simple and novel composition to maintain an individual or cell in a young state in a complex manner.

For this reason, one objective of the present invention is to provide a use of Rosa roxburghii fruit extract for preparing a cell rejuvenation composition, wherein the roxburghii fruit extract is obtained by extracting a roxburghii fruit with a solvent.

In an embodiment of the present invention, the solvent is water, alcohol, or a mixture of alcohol and water, the liquid-to-solid ratio of the solvent to the prickly pear is 5-20:1-5, and the extraction is performed at 50°C-100°C get on.

In an embodiment of the present invention, the prickly pear extract is a water extract of prickly pear, with a concentration of at least 0.5 mg/mL.

In an embodiment of the present invention, the prickly pear extract promotes the synthesis of telomerase, and enhances telomerase reverse transcriptase (TERT) and telomerase RNA component (TERC). ), or a combination of gene expression.

In one embodiment of the present invention, the prickly pear extract reduces the length of chromosomal telomeres that are shortened due to chemical drugs or ultraviolet radiation.

In an embodiment of the present invention, the prickly pear extract promotes gene repair and enhances the gene expression of a DNA repair protein selected from the group consisting of MPG (N-methylpurine DNA glycosylase, N-methylpurine DNA glycosylase), ERCC6 (excision repair cross complementing 6, nucleotide shear repair cross complementing protein 6), XRCC5 (X-ray repair cross complementing 5, X-ray repair cross complementing protein 5), and any combination thereof The group formed.

In an embodiment of the present invention, the prickly pear extract promotes the synthesis of a chaperonin containing TCP1 complex (CCT) and enhances the gene expression of a protein subunit of the chaperonin T complex. The protein subunit is selected from the group consisting of CCT2, CCT5, CCT6A, CCT7, CCT8, and any combination thereof.

In one embodiment of the present invention, the prickly pear extract enhances mitochondrial activity and inhibits adenosine monophosphate ribose polymerase (also known as poly(ADP-ribose) polymerase, PARP) The gene expression of the PARP line is selected from the group consisting of PARP1, PARP3, PARP4, PARP8, PARP11, and any combination thereof.

The present invention, based on the results of gene expression analysis, reveals that Prickly Pear extract can simultaneously reduce cell damage caused by oxidative stress or inflammation and maintain the normal functioning of genes, proteins, and mitochondria, and can stimulate the telomerase reaction and Reduce the damage of telomeres due to chemical drugs or ultraviolet radiation, thus promoting cell rejuvenation. Therefore, the present invention provides thorn pear extract for preparing cell rejuvenation combination The composition can be in the form of powder, granular, liquid, gel or paste, and can be made into food, drink, medicine, reagent or nutritional supplement, and it can be administered by oral administration, skin application, etc. individual.

The following will further illustrate the embodiments of the present invention in conjunction with the drawings. The following examples are used to illustrate the inventive features and applications of the present invention, rather than to limit the scope of the present invention. Anyone familiar with the art will not depart from Within the spirit and scope of the present invention, some changes and modifications can be made. Therefore, the scope of protection of the present invention shall be subject to those defined by the attached patent scope.

Figure 1 shows the comparison of superoxide dismutase 2 (SOD2) genes in THP-1 cells stimulated by lipopolysaccharide (LPS) after 24 hours of treatment with or without prickly pear extract compared to the superoxide dismutase 2 (SOD2) gene of control group cells Relative performance.

Figure 2 shows the CC motif chemokine ligand 3 (CCL3) gene of THP-1 cells stimulated by lipopolysaccharide (LPS) after 6 hours of treatment with or without prickly pear extract compared to the control group cells The relative performance.

Figure 3 shows that THP-1 cells stimulated by lipopolysaccharide (LPS) were treated with or without prickly pear extract for 6 hours, compared with control group cells for interleukin-1 receptor antagonist (IL). -1RA) and the relative expression levels of interleukin-10 (IL-10) genes.

Figure 4 shows the relative expression levels of MPG, ERCC6, and XRCC5 genes in THP-1 cells stimulated by lipopolysaccharide (LPS) after 6 hours of treatment with or without prickly pear extract relative to cells in the control group.

Figure 5 shows the relative expression levels of CCT2, CCT5, CCT6A, CCT7, and CCT8 genes in THP-1 cells treated with Cili extract for 6 hours or 24 hours relative to control group cells.

Figure 6 shows the relative expression levels of PARP1, PARP3, PARP4, PARP8, and PARP11 genes in THP-1 cells treated with Cili extract for 6 hours or 24 hours relative to control group cells.

Figure 7 shows the relative expression levels of TERT and TERC genes in THP-1 cells and control cells treated with Cili extract for 6 hours.

Fig. 8 shows the inhibitory effect of the water extract of Prickly Pear on the telomere shortening of skin cells caused by chemical drugs.

Fig. 9 shows the inhibitory effect of the water extract of Prickly Pear on the telomere shortening of skin cells caused by ultraviolet radiation.

The present invention provides a use of extracts of prickly pear for preparing a cell rejuvenation composition. The prickly pear extract of the present invention is obtained by extracting a prickly pear with a solvent, wherein the solvent is water, alcohol, or a mixture of alcohol and water, and the liquid-solid ratio of the solvent to the prickly pear is 5-20:1~ 5. And the extraction is carried out at 50℃~100℃. Based on gene expression analysis technology, the prickly pear extract has been proven to promote antioxidant-related genes, anti-inflammatory cytokine genes, DNA repair-related protein genes, protein subunit genes of the companion protein T complex (CCT), and telomeres The expression of genes related to enzyme reaction, and inhibit the expression of genes related to inflammation reaction and genes related to mitochondrial dysfunction, and reduce the length of chromosome telomeres due to chemical drugs or ultraviolet radiation.

definition

The numerical values used herein are approximate values, and all experimental data are expressed in the range of 20%, preferably in the range of 10%, and most preferably in the range of 5%.

The so-called "cell rejuvenation" in this article refers to the process of reversing the occurrence of cell aging. It can be judged by a variety of biological indicators, such as promoting the expression of genes involved in cell DNA repair, telomerase reaction, and the normal function of proteins, and promoting interaction with mitochondria. Gene expression of protein or RNA related to activity, and promotes chromosome telomere extension.

Materials and Methods Cell culture

The following examples use the human monocytic cell line THP-1 (ATCC TIB202) purchased from the American Type Culture Collection (ATCC) and the biological resource preservation purchased from the Food Industry Development Institute And the research center (Bioresource Collection and Research Center, BCRC) human skin fibroblast (human skin fibroblast) CCD-966SK (BCRC 60153). THP-1 cells were cultured in RPMI medium (RPMI medium 1640; Gibco) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (RPMI medium 1640; Gibco) under the condition of 37°C and 5% carbon dioxide. RPMI cell culture medium. CCD-966SK cells were cultured in a minimum basal medium (Minimum Essential Medium (MEM) (MEM); Gibco) supplemented with 10% FBS and 1% penicillin-streptomycin at 37°C and 5% carbon dioxide, hereinafter referred to as MEM cell culture medium.

Genome chip analysis

The human genome chip (manufactured by Hualian Biotechnology Co., Ltd.) is used to determine the changes in cell genome performance. The steps are briefly described as follows. According to the manufacturer’s instructions, RNA extraction kit (RNA Extraction Kit; Geneaid) was used to isolate RNA from THP-1 cells, and use it as a template to deoxygenate thymine with T7 promoter sequence at 37°C. The oligo dT primer is used for reverse transcription to synthesize the first complementary deoxyribonucleic acid (cDNA), and then the double-stranded cDNA is synthesized by DNA polymerase and RNase H (RNase H). After purification, the double-stranded cDNA was used as a template, and an RNA amplification kit (Amino Allyl MessageAmp ^TM aRNA Amplification kit; Invitrogen) was used for test tubes in an environment where amino allyl uridine triphosphate (amino allyl UTP, aaUTP) was added. In vitro transcription is used to synthesize aminoallyl-modified RNA (abbreviated as aRNA). The purified aRNA can be combined with an amino-reactive fluorescent dye such as Cy5 (Cy5 NHS ester; AAT Bioquest) to generate dye-labeled RNA. After removing the unbound dye and purifying the dye-labeled RNA by dialysis, it is added to the human genome chip loaded with a plurality of specific gene probes, and the hybridization kit (Phalanx) is used for processing. Hybrid reaction. The proteins encoded by the plural specific genes include antioxidant-related SOD2, inflammation-related CCL3, anti-inflammatory cytokines IL-1RA and IL-10, DNA repair-related proteins such as MPG, ERCC6, XRCC5, and associated protein T complex ( The various protein subunits of CCT) such as CCT2, CCT5, CCT6A, CCT7, and CCT8, and a variety of poly ADP ribose polymerases (PARPs) related to mitochondrial dysfunction, including PARP1, PARP3, PARP4, PARP8, and PARP11. The genome chip uses an Agilent microarray scanner to detect the heterozygous fluorescent signal after the hybrid reaction. The initial data were normalized to express the relative expression level of genes with log2 logarithmic value. The statistical analysis department uses the STDEV function in Excel software to calculate the standard deviation of the relative expression of each gene, and uses the one-tailed Student’s T test (TTEST) to calculate the statistical difference.

Gene expression analysis

Based on the quantitative polymerase chain reaction (qPCR), the gene expression level of telomerase reverse transcriptase (TERT) and telomerase RNA component (TERC) involved in the telomerase reaction in the cell is measured. The steps are briefly described below. According to the manufacturer's instructions, RNA was isolated from the cells using RNA Extraction Kit (Geneaid), and 2000ng of RNA was reverse-transcribed into cDNA with SuperScript® III Reverse Transcriptase (Invitrogen) at 37°C. Secondly, use the qPCR kit (KAPA CYBR FAST qPCR Kit (2X); KAPA Biosystems) and target genes such as TERT and TERC and glyceraldehyde 3-phosphate dehydrogenase as an internal control The primer pair of (Glyceraldehyde 3-phosphate dehydrogenase, GAPDH) gene (Table 1) was subjected to qPCR on the aforementioned cDNA in a PCR reaction machine (Step One Plus Real-Time PCR system; Applied Biosystems) to obtain a melting curve.

Finally, the 2- ^△△CT method was used to determine the relative expression level of the target gene. The method is the threshold cycle (C _T) GAPDH gene as an internal control of the reference cycle threshold gene, calculated by the following formula relative fold change: C gene of △ C _T = the experimental group or the control group _T - internal control C _T

△△ C _T = test group △ C _T - △ C _T of the control group

Multiple change=2 ^{-△△Ct average value}

The statistical analysis department uses the STDEV function in Excel software to calculate the standard deviation of the relative expression of each gene, and uses the one-tailed Student’s T test (TTEST) to calculate the statistical difference.

Telomere length determination

Telomere length is determined based on qPCR technology. First, a genomic DNA sample is extracted from the cells. Secondly, using the qPCR kit (KAPA CYBR FAST qPCR Kit (2X); KAPA Biosystems) and the primer pair of telomere and 36B4 gene (single copy number gene) (Table 2), the genomic DNA sample (20ng ) Perform qPCR to obtain melting curve. Meanwhile, telomere prepared (diluted 10 to ^106-fold from 60pg) and 36B4 gene (from 200pg diluted 10 to ^106-fold) of serially diluted standards, the following reaction conditions to obtain a standard curve of the qPCR both (Ct /kb): 10-minute denaturation stage (95°C), followed by 40 cycles of 15-second denaturation stage (95°C) and 1-minute bonding stage. The telomere as the standard product is an oligonucleotide with 14 repeats of TTAGGG (SEQ ID NO: 7); the 36B4 gene as the standard product has the nucleotide sequence of SEQ ID NO: 8. Finally, the average length of telomeres in the genomic DNA sample is calculated from the aforementioned standard curve, the telomere of the genomic DNA sample and the cycle threshold of the 36B4 gene.

Example 1 Preparation of Prickly Pear Extract

First, wash and dry the whole prickly pear fruit including the peel and thorns, and use a homogenizer to coarsely crush it. Secondly, the homogenate of Cili fruit is extracted with water, alcohol, or alcohol-water mixture as the solvent. The liquid-solid ratio of the mixture of the solvent and the homogenate of the prickly pear fruit is 5-20:1-5. The extraction temperature is between 50°C and 100°C, preferably between 70°C and 95°C. The extraction time in this embodiment is 0.5 to 3 hours.

After cooling the prickly pear extract obtained through the above extraction step to room temperature, it can be filtered with a 400 mesh filter to remove residual solids. The filtered prickly pear extract can be further concentrated under reduced pressure at 45°C to 70°C to obtain a concentrated product. In order to obtain a solid prickly pear extract, the foregoing prickly pear extract concentrated under reduced pressure can be spray-dried to remove the solvent, thereby obtaining the prickly pear extract powder. Before the spray-drying step, the extract of Prickly Pear can be selectively mixed with maltodextrin in a weight ratio (w/w) of 5-20:1-5.

Example 2 Prickly Pear extract reduces cell damage and promotes DNA repair and cell rejuvenation

In this example, the human genome chip was used to analyze the changes in the gene expression of the human monocyte cell line THP-1 after being treated with the water extract of the prickly pear. The same analysis was performed on the alcohol extract or alcohol water extract of Prickly Pear (data not shown). First, THP-1 cells ^{were seeded at 1.5×10 5} cells/well in each well of a 6-well plate containing 2 mL of RPMI cell culture medium. After culturing at 37°C for 24 hours, the cell culture medium was removed and washed with PBS solution cell. Afterwards, add 500 μL of 1 mg/mL prickly pear water extract and 500 μL of FBS-free RPMI cell culture medium to the cells in each well (experimental group), or only treat the cells with 500 μL of FBS-free RPMI cell culture medium as a control group . Cells in the experimental group were cultured at 37°C for 6 or 24 hours with or without stimulation of 10μg/mL lipopolysaccharide, and then used for gene expression analysis. The relative expression of the designated gene was compared with the same gene in the control group. The multiple of the expression after time is taken as the log2 value.

Figure 1 shows the relative expression level of SOD2 gene in THP-1 cells stimulated by lipopolysaccharide after 24 hours of treatment with or without the water extract of prickly pear, relative to the SOD2 gene of the control group. According to Figure 1, compared with THP-1 cells that were stimulated by lipopolysaccharide, the administration of Prickly Pear extract to the cells at the same time significantly improved the gene expression of SOD2. This result indicates that Prickly Pear extract can improve the ability of cells to remove reactive oxygen species. Therefore, it protects cells from oxidative stress, such as oxidative damage caused by free radicals from the cell itself and the environment.

Figure 2 shows the relative expression level of the CCL3 gene of THP-1 cells stimulated by lipopolysaccharide after 6 hours of treatment with or without the water extract of Prickly Pear, relative to the cells of the control group. Figure 3 shows the relative expression levels of IL-1RA and IL-10 genes in THP-1 cells stimulated by lipopolysaccharide after 6 hours of treatment with or without the water extract of Prickly Pear, relative to cells in the control group. According to Figures 2 to 3, compared with THP-1 cells stimulated by lipopolysaccharide alone, the administration of Prickly Pear extract to the cells at the same time significantly inhibits the expression of the CCL3 gene related to inflammation, and enhances the anti-inflammatory cytokine IL-1RA With the gene expression of IL-10, this result shows that Prickly Pear extract can inhibit immune cells to induce inflammation, thereby reducing individual cell damage due to excessive inflammation.

Figure 4 shows the relative expression levels of MPG, ERCC6, and XRCC5 genes in THP-1 cells stimulated by lipopolysaccharide after 6 hours of treatment with or without the water extract of Prickly Pear, relative to cells in the control group. According to Figure 4, compared with THP-1 cells that were stimulated by lipopolysaccharide alone, the administration of Prickly Pear extract to the cells at the same time significantly enhanced the gene expression of the aforementioned three DNA repair proteins. This result indicates that Prickly Pear extract can enhance the damage of cells. The repair ability of DNA or genes can maintain the normal operation of genes.

Figure 5 shows the relative expression levels of CCT2, CCT5, CCT6A, CCT7, and CCT8 genes in THP-1 cells treated with Cili water extract for 6 hours or 24 hours relative to control group cells. According to Fig. 5, the treatment of Prickly Pear extract enhances the gene expression of the concomitant protein T complex subunit of THP-1 cells, including CCT2, CCT5, CCT6A, CCT7, and CCT8 genes. This result indicates that Prickly Pear extract can increase the synthesis of the accompanying protein T complex that assists in protein folding, and is therefore beneficial to the normal structure and function of intracellular proteins.

Figure 6 shows the relative expression levels of PARP1, PARP3, PARP4, PARP8, and PARP11 genes in THP-1 cells treated with Cili water extract for 6 hours or 24 hours relative to control group cells. According to Figure 6, the treatment of Prickly Pear extract inhibits the gene expression of multiple poly ADP ribose polymerase (PARP) in THP-1 cells. In view of previous studies that the inhibition of this enzyme activity can improve mitochondrial activity, this result shows that Cili extract can increase mitochondrial activity, thereby improving cell energy production. Based on the experimental results shown in Figures 1-6, Cili extract has the effect of rejuvenating cells.

Example 3 Prickly pear extract enhances the expression of telomerase-related genes

Previous studies have revealed that increasing the telomerase activity in cells can promote cell growth. In order to explore the effect of Cili pear extract on the expression of genes related to telomerase reaction, qPCR was used to determine the telomerase reverse transcriptase (TERT) and terminal enzymes of human monocytic cell line THP-1 after treatment with Cili water extract. Changes in gene expression of granzyme RNA component (TERC). First, THP-1 cells ^{were seeded at 1.5×10 5} cells/well in each well of a 6-well plate containing 2 mL of RPMI cell culture medium. After culturing at 37°C for 24 hours, the cell culture medium was removed and washed with PBS solution cell. Afterwards, 500μL of 2mg/mL prickly pear water extract and 500μL of FBS-free RPMI cell culture medium were added to the cells in each well (experimental group), or only 500μL of FBS-free RPMI cell culture medium was used to treat the cells as a control group . The aforementioned two groups of cells were cultured at 37°C for 6 hours and then used for qPCR analysis.

Figure 7 shows the relative expression levels of TERT and TERC genes in THP-1 cells and control group cells treated with water extract of Cili pear for 6 hours. The relative expression level of the ordinate in the figure represents the RNA relative to the same gene in the control group cells. The multiple of the performance. According to Figure 7, the administration of Prickly Pear extract to THP-1 cells increased the expression levels of TERT and TERC genes by 2 times and 1.5 times, respectively. This result shows that Prickly Pear extract can increase the telomerase reaction in cells and lengthen telomeres, so it has the effect of prolonging cell life or delaying aging.

Example 4 Prickly pear extract inhibits the shortening of chromosome telomere length 4.1 Anti-chemical drug-induced telomere shortening

This example explores the protective effect of the water extract of Prickly Pear on the chromosome telomeres of human skin fibroblasts CCD-966SK. First, CCD-966SK cells ^{were seeded at 1.5×10 5} cells/plate on three 35mm culture plates each containing 2 mL of MEM cell culture medium. After culturing overnight at 37°C, half of the cells were taken from each plate (day 0). Installed in RNA stabilization solution (RNAlater solution; Ambion) and stored at -20°C for subsequent use. The remaining cells were respectively inoculated into three other 35mm culture plates and incubated at 37°C overnight. Afterwards, one plate of cells in the three plates was treated with 30μM chemotherapeutic drug etoposide (etoposide), and the other plate was treated with 30μM etoposide and 0.5mg/mL prickly pear water extract, and the remaining plate was not treated with etoposide. Treated with medicine and prickly pear extract as the control group. After 5 days of culture, the three groups of cells were collected (day 5). Genes were obtained from the cells collected on day 0 and day 5 by using the taco ^TM automatic nucleic acid extraction system (GeneReach Biotechnology) and the accompanying DNA extraction kit (taco ^{TM DNA/RNA extraction kit)} Body DNA, used for qPCR and telomere length determination. As shown in Figure 8, compared with the control group of cells, the treatment of Etopoxil significantly shortened the relative length of telomeres, but the treatment of the water extract of Prickly Pear can alleviate the shortening of telomeres, indicating that the extract of Prickly Pear can maintain The length of the chemically damaged telomere.

4.2 Resistance to telomere shortening caused by ultraviolet (UV) radiation

CCD-966SK cells ^{were seeded at 1.5×10 5} cells/plate on three 35mm culture plates each containing 2mL MEM cell culture medium. After culturing overnight at 37°C, the two plates of cells were given 1J/cm ² UVA (wavelength 320- 400nm) irradiation, another plate of cells was not irradiated with UVA and set as the control group. Take half of the cells from each culture plate (day 0) and aliquot them into RNA stabilization solution (RNAlater solution; Ambion) and store at -20°C for subsequent use. The remaining cells are respectively inoculated into three other 35mm culture plates at 37°C. Cultivate overnight. Afterwards, the cells in one of the two plates of UVA-irradiated cells were treated with 0.5 mg/mL prickly pear water extract, and the cells in the other plate were not treated. After two subcultures and repeat the aforementioned ultraviolet irradiation steps each time, the three groups of cells were collected (day 7). ^{GeneReach Biotechnology (Taco TM} automatic nucleic acid extraction system; GeneReach Biotechnology) and accompanying DNA extraction kit (taco ^TM DNA/RNA extraction kit) were used to obtain genes from the cells collected on day 0 and day 7. Body DNA, used for qPCR and telomere length determination. Figure 9 shows the inhibitory effect of water extract of Cili pear on the shortening of skin cell telomeres caused by ultraviolet radiation. As shown in Figure 9, compared with the control group cells, UVA irradiation significantly shortened the relative length of telomeres, but the treatment of the water extract of Cili pear can greatly reduce this phenomenon of telomere shortening, indicating that the extract of Cili pear can maintain the effect of ultraviolet rays. The length of damaged telomeres, that is, prickly pear extract has anti-photoaging effect.

In summary, the above experiments show that Cili extract can simultaneously reduce cell damage caused by oxidative stress or inflammation and maintain the normal functioning of genes, proteins, and mitochondria, and can increase telomerase reaction and reduce telomere due to chemistry. Damage caused by drugs or ultraviolet radiation will eventually lead to the rejuvenation of cells. Therefore, the present invention provides the use of Prickly Pear extract for preparing cell rejuvenation composition, which can be powder, granular, liquid, gel or paste, and can be made into food, drink, medicine, reagent Or nutritional supplements, given to a body by oral administration, skin application, etc.

<110> Dajiang Biomedical Co., Ltd.

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Claims (8)

A use of Prickly Pear extract is to prepare a pharmaceutical composition that promotes damaged gene repair in cells and normal protein folding, wherein the Prickly Pear extract is obtained by extracting a Prickly Pear with a solvent. The use described in item 1 of the scope of the patent application, wherein the solvent is water, alcohol, or a mixture of alcohol and water. The use described in item 1 of the scope of patent application, wherein the prickly pear extract enhances the gene expression of a DNA repair protein selected from the group consisting of MPG, ERCC6, XRCC5, and any combination thereof group. The use as described in item 1 of the scope of patent application, wherein the prickly pear extract promotes the synthesis of a concomitant protein T complex. The use as described in item 4 of the scope of patent application, wherein the prickly pear extract enhances the gene expression of a protein subunit of the companion protein T complex (CCT), and the protein subunit is selected from CCT2, CCT5, A group consisting of CCT6A, CCT7, CCT8, and any combination thereof. The use as described in item 1 of the scope of patent application, wherein the liquid-solid ratio of the solvent to the prickly pear is 5-20:1-5. For the purposes described in item 1 of the scope of patent application, the extraction is carried out at 50°C to 100°C. The use described in item 1 of the scope of the patent application, wherein the prickly pear extract is a water extract of prickly pear, the concentration of which is at least 0.5 mg/mL.

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