TWI712451B - Tip adaptor, biological detection device and biological detection method - Google Patents

Abstract

A tip adaptor, a biological detection device and a biological detection method are provided. The tip adaptor includes a tube-body, a filter plate and fibers, in which the filter plate and the fibers are disposed in the tube-body. The biological detection device includes a pipette, a tip, the tip adaptor, an exciting light source and an image capturing device. In the biological detection method, at first, the pipette is provided. Thereafter, the tip is disposed on the pipette, and the tip adaptor is disposed on the tip. Thereafter, the exciting light source and the image capturing device are used to determine if a portion of a liquid sample in a sample container includes a target. When the portion of the liquid sample includes the target, the pipette is used to drain the portion of the liquid sample in the sample container, thereby storing the target in the tip adaptor. Then, the pipette is controlled to release the target to a target container.

Description

Straw tip auxiliary device, biological detection device and biological detection method

The invention relates to a straw-tip auxiliary device, a biological detection device and a biological detection method.

With the development of biotechnology, biodetection technology is widely used in food, medical, public health and other related industries. In general biological detection technology, it is necessary to use a mixture of reagents and specimens to perform experiments or inspections, and most of the sampling or movement of liquid samples is performed by pipettes.

A pipette (or called a micropipette) can be used to draw and dispense a small amount of liquid samples to provide samples for various experiments. For example, a cell needle is combined to move the sample liquid to provide a cell sample required for biological testing. However, when a cell needle is used to draw a sample of the liquid, it is often necessary to draw again because the cells or cell clumps (microemboli) in the sample liquid are not drawn.

In order to solve the above-mentioned problems, one aspect of the present invention provides a straw tip assistive device, a biological detection device and a biological detection method.

According to some embodiments of the present invention, the aforementioned pipette tip accessory includes a tube body, a filter, a first optical fiber, and a second optical fiber. The pipe body includes an upper part and a lower part, and has a first through hole and a second through hole penetrating the upper part and the lower part. The upper part has a clamping part, the lower part has a first opening, and the clamping part has a second opening, wherein the second opening is designed to accommodate and clamp the straw. The filter is arranged at the lower part of the tube body to define a storage space to contain the sample liquid. The first optical fiber is arranged in the first through hole to receive the excitation light and transmit the excitation light to the target container. The second optical fiber is arranged in the second through hole for the image capturing device to capture the image of the target container.

In some embodiments, the inner diameter of the aforementioned first opening is between 15 microns and 50 microns.

In some embodiments, the outer diameter of the aforementioned first opening is substantially 200 microns.

In some embodiments, the aforementioned filter has filter holes, and the diameter of the filter holes is greater than 0 micrometers and less than or equal to 10 micrometers.

According to some embodiments of the present invention, the aforementioned biological detection device includes a pipette, a pipette tip, a pipette tip assistant, an excitation light source, and an image capture device. The pipette is used to sample the liquid. The pipette tip is set on the pipette. The straw tip auxiliary device is arranged on the straw tip, wherein the straw tip auxiliary device includes a tube body, a filter, a first optical fiber and a second optical fiber. The pipe body includes an upper part and a lower part, and has a first through hole and a second through hole penetrating the upper part and the lower part. The upper part has a clamping part, the lower part has a first opening, and the clamping part has a second opening, wherein the second opening is designed to accommodate and clamp the straw. The filter is arranged at the lower part of the tube body to define a storage space to contain a part of the sample liquid. The first optical fiber is disposed in the first through hole. The second optical fiber is disposed in the second through hole. The excitation light source is used for emitting excitation light to the first optical fiber so as to transmit the excitation light to the target container through the first optical fiber. The image capturing device is used to capture the image of the target container through the second optical fiber.

In some embodiments, the inner diameter of the aforementioned first opening is between 15 microns and 50 microns.

In some embodiments, the outer diameter of the aforementioned first opening is substantially 200 microns.

In some embodiments, the aforementioned filter has filter holes, and the diameter of the filter holes is greater than 0 micrometers and less than or equal to 10 micrometers.

According to some embodiments of the present invention, the aforementioned biological detection method includes: providing a pipette; arranging the pipette tip on the pipette; arranging the pipette tip auxiliary tool on the pipette tip; emitting excitation light to the first optical fiber to The excitation light is transmitted to a part of the sample liquid in the sample container through the first optical fiber; the image capturing device is used to capture the image of that part of the sample liquid in the sample container through the second optical fiber to determine whether the part of the sample liquid is Contains the target; when the part of the sample liquid contains the target, a pipette is used to suck the part of the sample liquid in the container, and a sub-part of the part of the sample liquid is contained in the storage space, wherein This sub-part contains the target; the pipette is moved to the target container to control the pipette to discharge only the sub-part of the sample liquid in the storage space to the target container. The pipette tip auxiliary device includes a tube body, a filter, a first optical fiber and a second optical fiber. The pipe body includes an upper part and a lower part, and has a first through hole and a second through hole penetrating the upper part and the lower part. The upper part has a clamping part, the lower part has a first opening, and the clamping part has a second opening, wherein the second opening is designed to accommodate and clamp the straw. The filter is arranged at the lower part of the tube body to define a storage space. The first optical fiber is disposed in the first through hole. The second optical fiber is disposed in the second through hole.

In some embodiments, the target is a cell or cell clump.

In order to make the above-mentioned features and advantages of the present invention more comprehensible, the following specific embodiments are described in detail in conjunction with the accompanying drawings.

Regarding the "first", "second", etc. used in this text, it does not specifically refer to the order or sequence, but only to distinguish elements or operations described in the same technical terms.

Please refer to FIG. 1A, which is a schematic structural diagram of a biological detection device 100 according to an embodiment of the present invention. The biological detection device 100 includes a pipette 110, a pipette tip 120, a pipette tip auxiliary device 130, an excitation light source 140, and an image capturing device 150. In FIG. 1A, the straw-tip assisting device 130 is shown in a cross-sectional structure and has a relatively large scale to facilitate the description of the function of the straw-tip assisting device 130. In fact, the size of the straw tip assist 130 is smaller than the size of the straw tip 120.

In this embodiment, the biological detection device 100 is installed in a large-scale biological detection equipment, such as a microscope equipment, to provide the movement and detection functions of the sample liquid. As shown in FIG. 1A, the pipette tip 120 is sleeved on the pipette 110, and the pipette tip assistant 130 is sleeved on the pipette tip 120. The pipette 110 has a shaft 112 and a control rod 114. The control rod 114 is used for the detection device to control the piston structure (not shown) of the pipette 110 to suck the sample liquid to the pipette tip auxiliary device 130 through the shaft 114 And the pipette tip 120, the sample liquid is contained in the pipette tip auxiliary device 130 and the pipette tip 120.

The pipette tip assistant 130 includes a tube body 132, a filter 134, and a first optical fiber FB1 and a second FB2. The pipe body 132 includes an upper portion 132a and a lower portion 132b. The upper part 132a has a clamping part 136 for receiving and clamping the straw tip 120. Specifically, the clamping portion 136 has an upper opening OP1, and the size of the upper opening OP1 is designed to match the straw tip 120, so that the clamping portion 136 can clamp the straw tip 120, and the straw tip assistant 130 is fixed to the straw tip 120 on. The lower part 132b has a lower opening OP2, and the lower opening OP2 has an inner diameter D1 and an outer diameter D2. In this embodiment, the outer diameter D2 is much larger than the inner diameter D1, so as to prevent the straw tip assist 130 from being damaged during use. For example, the inner diameter D1 can be between 15 microns and 50 microns, and the outer diameter D2 is substantially 200 microns. However, the embodiments of the present invention are not limited thereto. In other embodiments of the present invention, the user can determine the size of the lower opening OP2 according to the size of the object to be sucked.

The filter 134 is arranged at the lower part 132 of the tube body to define a storage space SP to contain a part of the sample liquid. In this embodiment, the filter 134 is designed with pores smaller than the cell size, so when the sample liquid is sucked into the pipette tip assisting device 130 through the lower opening OP2, the cells in the sample liquid will be blocked by the filter 134 and stay Storage space SP. In one embodiment, the diameter of the filter holes of the filter 134 is designed to be greater than 0 micrometers and less than or equal to 10 micrometers.

Please refer to FIG. 1A and FIG. 1B at the same time. FIG. 1B is a schematic top view of the straw-tip assisting device 130 according to an embodiment of the present invention. The tube body 132 has a first through hole TH1 and a second through hole TH2, and the first optical fiber FB1 and the second optical fiber FB2 are respectively disposed in the first through hole TH1 and the second through hole TH2.

In this embodiment, the tube body 132 is made of glass. For example, a piece of tapered glass material is provided first, and then the tapered material is processed by laser processing technology to obtain the tube body 132 as shown in FIG. 1A. Then, insert the filter 134 to the lower part 132b of the tube body 132, and insert the first optical fiber FB1 and the second optical fiber FB2 in the first through hole TH1 and the second through hole TH2, so that the pipette tip assistive device can be obtained 130. Among them, the filter 134 can be formed by laser processing technology, such as femtosecond laser processing technology.

The excitation light source 140 and the image capturing device 150 are used to detect the sample liquid. In one embodiment, when the user wants to move cells or cell masses (microemboli) from a sample container to a target container, the excitation light source 140 and the image capturing device 150 can be used to perform the Sample liquid for testing. For example, the excitation light source 140 emits excitation light LA to the first optical fiber FB1. After the first optical fiber FB1 receives the excitation light LA, it can transmit the excitation light LA to the sample container under the pipette tip assist 130 to excite the sample liquid in the sample container. Then, the image capturing device 150 is used to receive the light LC of the target container from the second optical fiber FB2 to capture the image of the sample liquid in the sample container. In this way, the part of the sample liquid to be sucked is detected first, and it can be judged whether the part of the sample liquid has cells or cell clumps.

Please refer to FIG. 2, which is a schematic flowchart of a biological detection method 200 using the biological detection device 100 according to an embodiment of the present invention. In the biological detection method 200, step 210 is first performed to provide a pipette 110. Then, step 220 is performed to set the pipette tip assistant 130 on the pipette 110. Then, step 230 is performed to put the straw tip auxiliary device 130 on the straw tip 120. In this embodiment, a plurality of straw tip assistive devices 130 are arranged on the assistive device frame AF in a matrix, as shown in FIG. 3. The detection equipment can move the pipette 110 to the auxiliary tool holder AF, and then move the pipette 110 down, so that the pipette tip 120 of the pipette 110 is inserted into the upper opening OP1 of the selected pipette tip auxiliary tool 130, The straw tip assisting device 130 thus selected can be fixed on the straw tip 120.

Next, proceed to step 240 to control the excitation light source 140 to emit the excitation light LA to the first optical fiber FB1 of the pipette tip accessory 130 to transmit the excitation light LA to the sample liquid 420 in the sample container 410 through the first optical fiber FB1 to excite The sample liquid 420 in the sample container 410 is as shown in FIG. 4A. Then, step 250 is performed to use the image capturing device 150 to capture an image of a part of the sample liquid 420 in the container 410 through the second optical fiber FB2 of the pipette tip assistant 130, as shown in FIG. 4B. Then, step 260 is performed to determine whether the part of the sample liquid 420 contains the target TA (for example, cells or cell clumps) based on the image obtained in step 250. If the part of the sample liquid 420 contains the target TA, step 270 is performed.

In step 270, the pipette 110 is used to suck up the aforementioned part of the sample liquid 420 to suck up the target TA, as shown in FIG. 4C.

In addition, depending on the amount of the part of the sample liquid 420 that is sucked, the part of the sample liquid 420 that is sucked may still be stored in the upper portion 132 a/the pipette tip 120 of the pipette tip assisting device 130. Since the filter 134 is designed with pores smaller than the cell size, the target TA will be stored in the sample liquid 420 (hereinafter referred to as the sub-part sample liquid) in the storage space SP, and other parts (for example, the upper part 132a of the pipette tip auxiliary device) The sample liquid 420 will have no cells.

Then, proceed to step 280 to move the pipette 110 to the target container 430 and control the pipette 110 to discharge only a sub-part of the sample liquid 420 (that is, the sample liquid 420 originally located in the storage space SP) into the target container 430 , As shown in Figure 4D. In this way, the function of moving the target TA from the sample container 410 to the target container 430 is realized. In some embodiments, in addition to the sample liquid 420 in the storage space SP, a part of the sample liquid 420 originally stored in the upper portion 132a of the pipette tip auxiliary device 130/the pipette tip 120 will also be discharged with the target TA.

In other embodiments of the present invention, when it is determined in step 250 that the detected part of the sample liquid 420 does not contain the target, the biological detection device 100 will be moved slightly, and then step 240 will be returned to compare other parts of the sample container 410 The sample liquid 420 is tested to find the target.

It can be seen from the above description that the pipette tip assist 130 of the embodiment of the present invention facilitates the biological detection method 200 to use the excitation light source 140 and the image capturing device 150 to detect the sample liquid 420. In the biological detection method 200 of the embodiment of the present invention, before the sample liquid is moved from the sample container to the target container, the part of the sample liquid to be sucked is first detected to determine whether the part of the sample liquid has a target, so as to ensure that all The part of the sample liquid that is sucked contains the target, so that the pipetting operation of the biological detection method 200 is performed more efficiently.

Although the present invention has been disclosed in the above embodiments, it is not intended to limit the present invention. Anyone with ordinary knowledge in the technical field can make some changes and modifications without departing from the spirit and scope of the present invention. The scope of protection of the present invention shall be determined by the scope of the attached patent application.

100: Biological detection device 110: Pipette 112: Axle 114: control lever 120: straw tip 130: Straw tip aids 132: Tube 132a: upper part 132b: lower part 134: filter 136: Clamping part 140: Excitation light source 150: Image capture device 200: Biological testing methods 210-280: steps 310: sample container 320: sample liquid 330: target container AF: Assistive tool rack D1: Inside diameter D2: Outside diameter FB1: First fiber FB2: second fiber LA: excitation light LC: Light of the target container OP1, OP2: opening SP: storage space TA: target TH1: First through hole TH2: Second through hole

Figure 1A shows a biological detection device according to an embodiment of the present invention. Fig. 1B is a schematic top view of the straw-tip assistive device according to an embodiment of the present invention. FIG. 2 is a schematic flowchart of a biological detection method using a biological detection device according to an embodiment of the present invention. FIG. 3 is a schematic top view of the structure of the auxiliary tool rack according to the embodiment of the present invention. 4A-4B are schematic diagrams of detecting sample liquid in a sample container according to an embodiment of the present invention. FIG. 4C is a schematic diagram of aspirating sample liquid in the container according to an embodiment of the present invention. FIG. 4D is a schematic diagram of discharging the sample liquid in the storage space according to an embodiment of the present invention.

no

100: Biological detection device

110: Pipette

112: Axle

114: control lever

120: straw tip

130: Straw tip aids

132: Tube

132a: upper part

132b: lower part

134: filter

136: Clamping part

140: Excitation light source

150: Image capture device

D1: Inside diameter

D2: Outside diameter

FB1: First fiber

FB2: second fiber

LA: excitation light

LC: Light of the target container

OP1, OP2: opening

SP: storage space

Claims (10)

A straw-tip assistive device suitable for a straw-tip, wherein the straw-tip assistive device includes: A pipe body includes an upper part and a lower part, and has a first through hole and a second through hole penetrating the upper part and the lower part, wherein the upper part has a clamping part, the lower part has a first opening, the The clamping portion has a second opening, and the second opening is designed to receive and clamp the straw tip; A filter is arranged at the lower part of the tube body to define a storage space to contain the sample liquid; A first optical fiber disposed in the first through hole to receive an excitation light and conduct the excitation light to a target container; and A second optical fiber is arranged in the second through hole for an image capturing device to capture the image of the target container. The pipette tip aid according to claim 1, wherein the inner diameter of the first opening is between 15 μm and 50 μm. The pipette tip assistive device according to claim 1, wherein the outer diameter of the first opening is substantially 200 microns. The pipette tip aid according to claim 1, wherein the filter has a filter hole, and the diameter of the filter hole is greater than 0 micrometers and less than or equal to 10 micrometers. A biological detection device, including: A pipette to suck a sample of liquid; A pipette tip is set on the pipette; A straw-tip auxiliary device is arranged on the straw-tip, wherein the straw-tip auxiliary device includes: A pipe body includes an upper part and a lower part, and has a first through hole and a second through hole penetrating the upper part and the lower part, wherein the upper part has a clamping part, the lower part has a first opening, the The clamping portion has a second opening, and the second opening is designed to receive and clamp the straw tip; A filter is arranged at the lower part of the tube body to define a storage space to contain the sample liquid; A first optical fiber disposed in the first through hole; and A second optical fiber arranged in the second through hole; An excitation light source for emitting an excitation light to the first optical fiber to transmit the excitation light to a target container through the first optical fiber; and An image capturing device is used to capture an image of the target container through the second optical fiber. The biological detection device according to claim 5, wherein the inner diameter of the first opening is between 15 μm and 50 μm. The biological detection device according to claim 5, wherein the outer diameter of the first opening is substantially 200 micrometers. The biological detection device according to claim 5, wherein the filter has a filter hole, and the diameter of the filter hole is greater than 0 micrometers and less than or equal to 10 micrometers. A biological detection method including: Provide a pipette; Set a pipette tip on the pipette; A straw tip assistive device is arranged on the straw tip, wherein the straw tip assistive device includes: A pipe body includes an upper part and a lower part, and has a first through hole and a second through hole penetrating the upper part and the lower part, wherein the upper part has a clamping part, the lower part has a first opening, the The clamping portion has a second opening, and the second opening is designed to receive and clamp the straw tip; A filter set on the lower part of the tube body to define a storage space; A first optical fiber disposed in the first through hole; and A second optical fiber arranged in the second through hole; Emitting an excitation light to the first optical fiber to transmit the excitation light to a part of a sample liquid in the sample container through the first optical fiber; Using an image capturing device to capture an image of the portion of the sample liquid in the sample container through the second optical fiber to determine whether the portion of the sample liquid contains a target; When the part of the sample liquid contains the target, the pipette is used to suck the part of the sample liquid in the sample container, and a sub-part of the part of the sample liquid is accommodated in the storage space , Wherein the sub-part contains the target; and The pipette is moved to a target container to control the pipette to discharge only the sub-portion of the sample liquid located in the storage space to the target container. The biological detection method according to claim 9, wherein the target object is a cell or a cell mass.

Images