TWI682718B - Suspended anti-mosquito bait and preparation method thereof - Google Patents

Abstract

The invention relates to a suspension type anti-mosquito bait and its preparation method. , Anhydrous glycerin and secondary water to prepare a mixed solution of embedded shell material. Adding the powder of sulibacterium to the mixed solution of embedding shell material to prepare the mixed solution of sulibacterium. The mixed solution of thuringiensis was instilled into an aqueous solution of calcium chloride, and sodium alginate and calcium chloride were contacted and cross-linked to form calcium alginate and coated with thuringiensis to make thuluibacterium microsphere capsules. Suspended bacteria microsphere capsules are sprayed and combined on the beeswax layer, dried and solidified to prepare a suspended artificial bait. The suspended artificial bait includes a beeswax layer and a Sulibacterium microsphere capsule combined with the beeswax layer. The Suli bacteria microsphere capsule includes Suli bacteria particles and an embedded shell material coated on the surface of the Suli bacteria particles. The embedding shell material contains sodium alginate, konjac powder, alkali powder and anhydrous glycerin.

Description

Suspended anti-mosquito bait and preparation method thereof

The invention relates to a suspension type mosquito killing bait and a preparation method thereof, in particular to a technique for preparing suspension artificial bait by microencapsulation of thuringiensis.

There are many infectious diseases using mosquitoes as vectors, such as dengue fever, hemorrhage of dengue fever, and Zika virus. These infectious diseases have caused many deaths. Most of the infectious diseases are only sprayed with insecticides as the treatment method, but vector mosquitoes have not. It is effectively cured. There are many ways to kill mosquitoes. Most people will use physical methods such as mosquito coils and mosquito trap lights, or chemical methods such as anti-mosquito liquids and spraying of chemicals. Although these methods can repel and kill mosquitoes, they hide another harmful factor. For example, mosquito coils, the smoke released from the burning of mosquito coils contains possible carcinogens (for example: cyclic aromatic hydrocarbons (PAHs)). Although the anti-mosquito liquid is convenient to carry, the ingredients contain DEET (a toxic substance that contacts the mucosal tissues and wounds, which may cause neurotoxicity).

It is commonly used in science to kill mosquitoes, Bacillus thuringiensis (scientific name: Bacillus thuringiensis), referred to as Suly bacteria. The protein crystals of toxins with insecticidal activity that can be secreted by S. utilis are used to kill Lepidoptera (eg butterfly), Diptera (eg mosquito), Coleoptera insects (eg beetle).

According to the investigation, there is a patent as shown in the new type announcement No. M387485, which The purpose of the invention of the patent is that the water trough is used for female mosquitoes to lay eggs; the long tube vertically protrudes from the bottom of the water trough to extend the opening of the water trough and is used for injecting a certain amount of water, the top of the long tube has a pulley and the opposite side wall is provided with a long trough; It is set on the long tube; the buoy is used to float on the water surface of the long tube; the traction line is set on the pulley and one end is connected to the buoy; the movable clamping column is lighter than the buoy and is located above the buoy and connected to the other end of the traction line, and has two elastic parts Pass through the long slot to resist the lowermost net cover respectively, the pontoon pulls the movable card column upwards as the water surface evaporates and moves downwards, after a period of time, the elastic member can be detached from the lowermost net cover, the net cover falls down and presses in The water causes it to die of hypoxia, and thus cannot reproduce. The present invention uses biological pesticides to sprinkle in water to cause poisoning and death if swallowed. Therefore, the technical means and effects achieved in this case are different from the patent.

There is also a new type of patent No. M551623, which is a structure that combines the function of salinomycin biopesticide and plant growth-promoting bio-fertilizer, which includes a core part; a sandwich part, which covers the The outer side of the core part and a shielding layer part cover the outer side of the interlayer part. Through the above structure, the initial biological pesticide used as an insecticide will naturally fall into the soil after a period of use, and further be used as a biological fertilizer to achieve the purpose of combining biological pesticides and biological fertilizers. The technology of the present invention adopts the sodium alginate mixed solution as the shell material, and covers the threshing bacteria in a single layer. Therefore, the technical means and effects achieved in this case are different from the patent.

The first object of the present invention is to provide a suspension type mosquito killing bait and its preparation method, which mainly adopts the preparation method of microcapsulation of thuringiensis, so it has no pollution to the environment, no harm to the human body and environmental ecology and only to specific insects ( Lepidoptera, Diptera, Coleoptera Etc.) effective. The technical means to achieve the cost goal is to prepare powdered spirulina, sodium alginate, konjac powder, alkali powder, anhydrous glycerin and secondary water; prepare sodium alginate, konjac powder, alkali powder, anhydrous glycerin and secondary water Into a mixed solution of embedded shell material; add the powder of sulibacterium to the mixed solution of encapsulated shell material to prepare a mixed solution of spirulina; drop the mixed solution of sulibacterium into an aqueous solution of calcium chloride, sodium alginate and chlorination Calcium contacts and cross-links to form calcium alginate and coats the spore bacteria to make suli bacteria microsphere capsules; the suli bacteria microsphere capsules are sprayed and combined on the beeswax layer and dried and solidified to prepare suspended artificial bait.

The second object of the present invention is to provide a better method for preparing suspension type bait for killing mosquitoes. The technical means to achieve the cost goal is to prepare powdered spirulina, sodium alginate, konjac powder, alkali powder, anhydrous glycerin and secondary water; prepare sodium alginate, konjac powder, alkali powder, anhydrous glycerin and secondary water Into a mixed solution of embedded shell material; add the powder of sulibacterium to the mixed solution of embedded shell material to prepare a mixed solution of sulibacterium; drop the mixed solution of sulibacterium into an aqueous solution of calcium chloride, sodium alginate and chlorination Calcium contacts the cross-chain to form calcium alginate and coats the spore bacteria to make sulibacterium microsphere capsules; the sulibacterium microsphere capsules are sprayed and combined on the beeswax layer, and after drying and solidification, a suspended artificial bait is prepared. Among them, the preparation of sulibacterium microsphere capsules is to fill the mixed solution of sulibacterium in a syringe, the syringe is erected on an electrostatic pumping device, and a beaker containing the calcium chloride aqueous solution is placed under one needle of the syringe , A metal plate is placed under the beaker as an electrode, two metal clips respectively connected to a positive pole and a negative pole of a power supply are respectively clamped to the needle and the metal plate, the power supply is turned on, and a creeping help is used Pu actuates the syringe, so that the mixed solution of thuringiensis filled in the syringe is discharged from a needle tip of the needle and dripped into the calcium chloride aqueous solution.

The third object of the present invention is to provide a suspension type bait for killing mosquitoes. The technical means to achieve the cost goal is to prepare powdered spirulina, sodium alginate, konjac powder, alkali powder, anhydrous glycerin and secondary water; prepare sodium alginate, konjac powder, alkali powder, anhydrous glycerin and secondary water Into a mixed solution of embedded shell material; add the powder of sulibacterium to the mixed solution of encapsulated shell material to prepare a mixed solution of spirulina; drop the mixed solution of sulibacterium into an aqueous solution of calcium chloride, sodium alginate and chlorination Calcium contacts and cross-links to form calcium alginate and coats the spore bacteria to make suli bacteria microsphere capsules; the suli bacteria microsphere capsules are sprayed and combined on the beeswax layer and dried and solidified to prepare suspended artificial bait. Wherein, the suspended artificial bait includes a solidified beeswax layer and a plurality of Sulibacterium microsphere capsules combined on the beeswax layer; each Sulibacterium microsphere capsule includes a Sulibacterium microsphere and coated on the Sue An embedded shell material on the surface of the viable bacteria particles. The embedded shell material contains the sodium alginate, the Konjac powder, the alkali powder and the anhydrous glycerin.

1‧‧‧glass plate

1a‧‧‧beeswax layer

1b‧‧‧Sully bacteria microsphere capsule

10‧‧‧syringe

11‧‧‧ Needle

12‧‧‧ syringe

20‧‧‧Electrostatic pumping device

21‧‧‧ Peristaltic Pump

30‧‧‧Calcium chloride aqueous solution

31‧‧‧Solid bacteria mixed solution

40‧‧‧Beaker

50‧‧‧Metal plate

60‧‧‧Power supply

d1‧‧‧Distance

FIG. 1 is a schematic flow chart of the manufacturing method of the present invention.

FIG. 2 is a schematic diagram of the present invention using an electrostatic pumping device as an electric field to drip a mixed solution of thuringiensis.

Fig. 3 is a schematic view of spraying and combining the Sulibacterium microsphere capsules on the beeswax layer of the present invention.

4 is a schematic cross-sectional view of the structure of the Sulibacterium microsphere capsule manufactured by the present invention.

FIG. 5 is a schematic cross-sectional view of forming a Sulibacterium microsphere capsule and a beeswax layer on a glass sheet of the present invention.

6 is a schematic view of the suspended artificial bait produced by the present invention after cutting is completed.

FIG. 7 is a schematic diagram of the entity picture of the Sulibacterium microsphere capsule manufactured by the present invention.

In order to enable your reviewing committee to further understand the overall technical characteristics of the present invention and the technical means to achieve the purpose of the invention, the specific embodiments and drawings are used to explain in detail as follows: The present invention uses embedding technology to microencapsulate Sulibacter , And the sodium alginate (sodium alginate) aqueous solution and calcium chloride (calcium chloride) aqueous solution using gel principle to react, when the sodium alginate solution encounters the calcium chloride solution for cross-linking reaction to form microcapsules, reuse The carrier with low adsorption force and low specific gravity adsorbs the microcapsules, so that the microcapsules can be suspended in water. The specific implementation of the present invention also uses S. utilis for research. The natural bactericide used in the present invention is sulibacterium, which is miniaturized by grinding. Then, food-grade materials are used to produce materials that can only decompose in the lye environment secreted by the intestinal tract. The shell materials are coated with sulibacteria, and the sulibacteria are coated into microcapsules. Then, using the cement with a specific gravity less than water, the microcapsules of sulibacterium are glued together, so that the prepared anti-mosquito bait can be suspended in the water. In the future, after the suspension-type anti-mosquito bait of the present invention is delivered, the shell material will be decomposed only under the lye condition. In case of entering the human body through the food chain, the human body secretes gastric acid and does not break down the shell material of the microcapsules, thus reducing the harm to people and the ecological environment.

Please refer to FIGS. 1-7 for achieving the first object of the present invention. A specific embodiment of the present invention of an environmentally friendly suspension-type artificial bait that can be biodegraded includes the following steps:

Step (a): preparing materials, namely preparing a specific amount of secondary water, a specific amount of edible sodium alginate, a specific amount of edible konjac powder, a specific amount of edible caustic soda powder, a specific amount of Edible glycerin and a specific amount of sulibacterium, a specific amount of edible beeswax.

Step (b): prepare a mixed solution of the embedded shell material, that is, eat a specific amount of Sodium alginate is added with a certain amount of secondary water, after stirring for a certain time, adding a certain amount of edible Konjac powder, after stirring for a certain time, adding a certain amount of edible alkali powder, after stirring for a certain time, adding A certain amount of edible glycerin is stirred for a certain period of time, and then prepared as an embedded shell material mixed solution.

Step (c): prepare the mixed solution of thuringiensis 31, that is, add a specific amount of thuluibacterium to the mixed solution of the embedding shell material, and after stirring for a specific time, prepare the mixed solution 31 of thuringiensis.

Step (d): Instilling cross-chains to prepare S. fulicile microspheres. After filling the S. bacterium mixed solution 31 into the syringe, use the syringe to inject the S. bacterium mixed solution 31 into 20~45% calcium chloride In the aqueous solution 30, the sodium alginate in the mixed solution 31 of thuringiensis contacts calcium chloride to produce a cross-linking, so that the calcium alginate coats the thuluibacterium, and after drying, the thalassium microspheres are made; and steps (e) Make a suspended artificial bait, that is, spray the Sulibacter microspheres on a glass plate 1 with a layer of liquid edible beeswax layer 1a with a thickness of about 0.5~2mm. After the beeswax solidifies, the Sulibacter microspheres combine On the beeswax layer 1a, as shown in FIG. 5, the beeswax layer 1a combined with the S. bacterium microspheres is taken off the glass plate 1, and cut into a desired size by planting to make artificial bait, as shown in FIG. 6. .

The fineness of the sulibacterium used in the present invention is about 2 μm because of the oesophagus, so the sulibacterium is ground to a particle size of less than 2 μm by using an airflow pulverizer and a planetary ball mill.

The specific manufacturing process of the Sulibacterium microcapsule of the present invention includes the following steps:

Step (a): Preparation of materials, that is, preparation of powdered spirulina, sodium alginate, konjac powder, alkali powder and secondary water, in which spirulina powder accounts for 10 to 100 parts by weight and the average particle size is less than 2 μm , Sodium alginate accounts for 0.5~1 parts by weight, Konjac powder accounts for 0.1~0.5 parts by weight, and alkali powder accounts for 0.1~0.5 parts by weight, anhydrous glycerin accounts for 1~5 parts by weight, 50~300 parts by weight (about 50~300ml) of secondary water.

Step (b): prepare a mixed solution of the embedded shell material, that is, 0.5 to 1 part by weight of sodium alginate, 0.1 to 0.5 part by weight of Konjac powder, 0.1 to 0.5 part by weight of alkali powder and 1 to 5 part by weight of anhydrous Glycerin is first added to 50~300 parts by weight of secondary water, mixed in a beaker 40, and stirred to 0.5~1 parts by weight of sodium alginate, 0.1~0.5 parts by weight of Konjac powder, 0.1~0.5 parts by weight of The alkali powder and 1 to 5 parts by weight of anhydrous glycerin are completely dissolved in 50 to 300 parts by weight of secondary water, and then prepared as an embedded shell material mixed solution.

Step (c): Prepare the mixed solution of S. spp. 31, then add 10 to 100 parts by weight of S. spp. powder to the 51.7 to 307 parts by weight of the embedded shell material mixed solution, and continue to stir for a few minutes After that, a mixed solution 31 of thuringiensis was further prepared.

Step (d): Injecting the cross-chain to prepare the Sulibacterium microsphere capsule 1b. Next, after the Sulibacterium mixed solution 31 is loaded into the syringe 12, the Sulibacterium mixed solution 31 is injected into the syringe 36 with the syringe 12 In the 100% calcium chloride aqueous solution 30, the sodium alginate in the mixed solution 31 of Sulfur bacteria contacts the calcium chloride to produce a cross-linking, so that the calcium alginate coats the Sully bacteria, and the dried bacteria are made into Sully bacteria The microsphere capsule 1b is shown in FIG.

Step (e): Make a suspended artificial bait, that is, dissolve beeswax (density about 0.958-0.970g/cm ³ ) on a clean glass surface, and then spray the embedded Sulibacter microsphere capsule 1b Lay a glass plate 1 on the melted beeswax layer 1a with a thickness of about 0.5~2mm, and then lay tape 1c on the edge of the beeswax layer 1a to determine the bait range and thickness, as shown in Figure 3, to be combined The beeswax layer 1a with the Sulibacterium microsphere capsule 1b is dried and solidified as shown in FIG. 5, and then the whole piece of the beeswax layer 1a containing the Sulibacterium microsphere capsule 1b is peeled off from the glass plate 1, and then cut into The required size, then complete the production of suspended artificial bait, as shown in Figure 6.

Among them, in the preferred embodiment, step (d), the syringe 10 can be erected on the electrostatic pumping device 20 (as shown in FIG. 1 ), and a beaker 40 containing an aqueous solution of calcium chloride 30 is placed under the needle 11. Next, place a metal plate 50 as an electrode, and then clamp the metal clip connected to the positive electrode to the needle 11, and the metal clip connected to the negative electrode to the electrode of the metal plate 50 below the beaker 40, turn on the power supply 60, use The peristaltic pump 21 advances the syringe 10 to discharge the mixed solution 31 of thuringiensis from the tip of the needle, and the peristaltic pump 21 with a voltage of 15KV controls the flow rate of the syringe 10 to advance to 50ml/min, and the end of the needle 11 and the beaker 40 The height distance d1 of the liquid surface is 40cm. Using the principle of positive and negative electrical attraction and the action of the electric field formed between them, when the droplet is released from the tip of the needle 11, because the end of the needle 11 is positively charged, it will form at high voltage Taylor cone droplets with positive charge. Due to the electric field, the droplets released from the tip of the needle 11 will rush into the beaker 40 containing 30100ml of 36% calcium chloride aqueous solution at high speed. When the sodium alginate in the mixed solution 31 of bacterium is in contact with calcium chloride, it will immediately cross-link to form calcium alginate to coat the bacterium and form the microsphere capsule 1b (particle size less than 2μm, such as (Figure 2). The sifted microsphere capsule 1b after sieving is placed in an oven at a temperature of about 40 to 70 degrees Celsius and dried into powder for standby, and the drying time is 8 to 12 hours.

In order to test the disintegration characteristics of Sulibacterium microspheres capsule 1b, the present invention carried out a disintegration test of Sulibacterium microspheres capsule 1b, first poured the Sulibacterium mixed solution into a petri dish (made into a film) , Dried in an oven at 40°C, then tear off the dried film, cut it into small pieces, and put them into solutions with pH=2.3, 6.8 and 9.5 respectively, and then observe the disintegration status. After a period of time, it was found that at pH=2.3 In the solution, Sulibacterium microsphere capsule 1b will not disintegrate. In a solution with a pH of 6.8, Sulibacterium microsphere capsule 1b will not disintegrate. Finally, in a solution with pH=9.5, Sulibacterium microsphere capsule 1b disintegrates, it can be seen that as long as the strong bacteria microsphere capsule 1b is not poured into a strong alkali solution, it will not disintegrate.

After the above specific description, the present invention does have the following characteristics:

1. The present invention uses a food-grade material to make a material that will decompose only in the lye environment secreted by the intestinal tract as a shell material coated with spores.

2. It can be embedded into microcapsules.

3. Reuse the cement with a specific gravity less than that of water to adhere the microcapsules of sulibacterium so that the produced carrier can be suspended in water.

4. After the suspension type anti-mosquito bait is delivered in the future, the shell material will only decompose under the condition of lye. In case the human body enters the human body through the food chain, the body will secrete stomach acid and the shell material of the microcapsule will not be broken down. Therefore, the harm to people and the ecological environment is reduced.

The above is only a feasible embodiment of the present invention and is not intended to limit the patent scope of the present invention. Any equivalent implementation of other changes based on the content, features and spirit described in the following claims should be Included in the patent scope of the present invention. The structural features of the invention specifically defined in the claim are not found in similar items, and are practical and progressive. They have met the requirements of the invention patent. You have filed an application in accordance with the law, and I would like to ask the Jun Bureau to approve the patent in accordance with the law to maintain this. The applicant's legal rights and interests.

1‧‧‧glass plate

1a‧‧‧beeswax layer

1b‧‧‧Sully bacteria microsphere capsule

Claims (9)

A method for making suspended artificial bait, which includes: step (a) preparing materials, that is, preparing 10-100 parts by weight of powdered S. sphaeroides with an average particle diameter of less than 2 μm, 0.5-1 parts by weight of sodium alginate, 0.1~0.5 parts by weight of Konjac powder, 0.1~0.5 parts by weight of alkali powder, 1~5 parts by weight of anhydrous glycerin and 50~300 parts by weight of secondary water; Step (b) prepare a mixed solution of embedded shell material, which is about to 0.5~1 parts by weight of sodium alginate, the 0.1~0.5 parts by weight of Konjac powder, the 0.1~0.5 parts by weight of alkali powder and 1~5 parts by weight of anhydrous glycerin are first added to the 50~300 parts by weight of secondary Water, stir and mix in a beaker to prepare a mixed solution of embedded shell material; Step (c) prepare a mixed solution of thuringiensis, that is, add the 10 to the mixed solution of embedded shell material of 51.7~307 parts by weight ~100 parts by weight of S. pulveris powder, after continuous stirring for a few minutes, and then prepare a S. pallidum mixed solution; Step (d) instill the cross-chain to prepare S. pallidum microsphere capsules, the S. pallidum mixed solution After loading into a syringe, the mixed solution of thuringiensis was dropped into 20 to 45% calcium chloride aqueous solution with the syringe, so that the sodium alginate in the thuluibacterial mixed solution was in contact with the calcium chloride Cross-linking occurs to make the calcium alginate coat the sulibacterium, and after drying, a plurality of sulibacterium microsphere capsules are made; and step (e) is made into a suspended artificial bait, that is, before the surface of a glass plate Lay a layer of molten liquid beeswax with a thickness of 0.5~2mm, and then spray the plurality of Sulibacter microsphere capsules on the beeswax layer, after the beeswax layer combined with the Sulibacter microsphere capsules is dried and solidified, After peeling off the whole piece of the beeswax layer containing the thuringiensis microsphere capsules from the glass plate, the preparation of a suspended artificial bait is completed. The method for making suspended artificial bait according to claim 1, wherein in step (a), the sodium alginate is edible sodium alginate, the konjac powder is edible konjac powder, and the alkali powder is edible Base powder, the anhydrous glycerin is edible anhydrous glycerin. The method for making suspended artificial bait according to claim 1, wherein in step (a), the secondary water is 200 parts by weight, the sodium alginate is 0.8 parts by weight, and the Konjac powder is 0.3 parts by weight, the The alkali powder is 0.3 parts by weight, the anhydrous glycerin is 3 parts by weight, and the powdered S. utilis is 50 parts by weight; the calcium chloride aqueous solution concentration is 36%; in step (e), the density of the beeswax layer is about 0.958 -0.970g/cm ³ . The method for making suspended artificial bait according to claim 1, wherein in step (b), the sodium alginate is added to the secondary water first, and then the Konjac powder is added after stirring, and the alkali powder is added after stirring, After stirring, the anhydrous glycerin is added, and after stirring, it is the mixed solution of the embedded shell material. The method for making a suspended artificial bait according to claim 1, wherein in step (d), the syringe is set up on an electrostatic pumping device, and a solution containing the calcium chloride aqueous solution is placed under one needle of the syringe A beaker, under which a metal plate is placed as an electrode, two metal clips respectively connected to a positive pole and a negative pole of a power supply are respectively clamped to the needle and the metal plate, and the power supply is turned on, using a The peristaltic pump actuates the syringe, so that the mixed solution of thuringiensis filled in the syringe is discharged from a needle tip of the needle and dripped into the calcium chloride aqueous solution. The method of making a suspended artificial bait as described in claim 5, wherein in step (d), the peristaltic pump controls the syringe to advance so that the flow rate of the mixed solution of thuringiensis is 50 ml/min, and the end of the needle is The height distance of the liquid surface of the mixed solution of thuringiensis in the beaker is 40 cm. The method for making suspended artificial bait as described in claim 1, wherein in step (d), the drying temperature of the dried microbe capsules is about 40-70 degrees Celsius, and the drying time is 8 ~12 hours. A suspended artificial bait prepared by the method according to claim 1, which comprises a solidified beeswax layer and a plurality of Sulibacter microsphere capsules bonded to the beeswax layer; each Sulibacter microsphere gel The capsule includes a spore bacteria particle and an embedding shell material coated on the surface of the spore bacteria particle. The embedding shell material includes the sodium alginate, the Konjac powder, the alkali powder and the anhydrous glycerin. The suspended artificial bait according to claim 8, wherein the average particle size of the plurality of S. bacterium microsphere capsules is less than 2 μm.

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